Strain
Avian_AY646283
(Region: ; Strain: Avian infectious bronchitis virus partridge/GD/S14/2003, complete genome.; Date: )
Gene
replicase 1a
Description
Annotated in NCBI,
replicase 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTTCAAGCCTAAAACAGGGAGTATCTTCCCAAAACCAGAGGAGTGTCGTTCTTGTTGCCAAAGACATTCCCGATCAACTCCGTGACGCTTTGTTTTTCTATACGTCACATAACCCAAAAGATTACGCTGATGCTTTCGCTTTTAAGCGGAAGTTTGATCGTAATCTGGGCGCTGGGAAGCAGTTTAAATTTGAAACTGTGTGTGGTCCATTCTTTCTTAAAGGAGTGGATAAAGTAACACCAGGGGTCCCAGCTAAAGTTTTGAAAGCCACTTCAAAATTGGCTGATTTAGAAGACATCTTTGGTGTCTCACCTTTTGCACGGAAATACCGTGAACTTCTTAAAACAGCATGCCAGTGGTCTCTTACTGTAGATGCGCTGAATGCACGCGCCCAAACTCTTGAAGAAATTTTTGGACCAACTGAAGTACTTTGGCTCCAGGTGGCTTCAAAACTTCAGGTTTCAGCTATGGCAATGCGCAGACTTGTTGGTGAAGTAACTGCAAAAGTCATGGATGCTTTGGGCTCAAAGTTGAGTTCTCTTTTTCAAATTGTGAAACAGCAAATAGCCAGAATTTTTCAGAAAGCTCTGGCTATTTTTGAAGATGTAAAAGACTTACCACAACGCATAGCTGCACTGCAGATGGCTTTTGCAAAGTGCGCTAAAACAATTACTGTTGTTGTTGTGGAAAAAGCTATTGTTGTTCGCGAATTTTCAGGAACTTGTCTTGCAAGTATTAATGGTGCCATTGCTAAGTTCTTTGCGGAACTACCCAATGGTTTTATGGGATCTAAAGTTTTTAGCACGCTGGCTTTCTTTAAAGAGGCAGCTGTTAAAATTGTAGAGAATGTCCCAGGAGCACCGCAAGGTACTAAAGGTTTTGAAGTTGTTGGCAATGCCAAAGGCACACAAGTTGTAATTCGTGGCATGCGAAATGACTTAACACTGCTTGACCAAAAAGCTGAGATTCCTGTTGAACCAGAGGGCTGGTCTGCAATTATGGATGGACATCTTTGTTATGTCTTTAAGAGTGGTGACAGATTTTATGCTGCGCCTTTGTCAGGAAACTTTGCACTTCATGATGTGCATTGCTGTGAACGTGTTGTCTGTCTTGTTGATGGTGTTACACCGGAGATTAATGACGGACTCATTCTTGCAGCAATCTACTCGTCATTTAGTGTGTCGGAACTTGTGGCTGCACTTAAGAAAGGTGAACCATTCAAGTTTCTAGGCCATAAGTTTGTGTATGCAAAGGAAGCTGCTGTCTCCTTTACATTGGCAAAAGCAGCTACTATTTCTGATGTCTTGAAGCTGTTTCAATCTGCTAGAGTAGAGACAGAGGATGTGTGGTCTGCACTTACTGAAAAGTCTTTTGAATTCTGGAAACTCGCATATGGAAAAGTGCGAAATTTAGAGGAATTTGTGAAGACTCATTTCTGTAAAGCCCAAATGTCAATTGTTGTTCTAGCAGTAGTGCTTGGTGAAGGCATTTGGCATCTTGTTTCTCAAGTCATCTATAGGTTGGGTTGTTCGTTTTCTAAAGTTATTGATTTTTGTGCAAAGTACTGGAAAGGTTTCTGCGGACAATTGAAGAAGGCTAAGCTTCTTGTCACAGAACACCTATGTGTCCTGAAAGGTGTTGCACAGCATTGTTTTCAACTTTTGCTGGATGCAATACATTCTATGTACAAGAGCTTTCAAAAATGTGCACTTGGTAGAATCCATGGAGATTTACTCTTCTGGAAAGGAGGTGTACACAAAGTAGTTCAGGAAGGCGACGAAATTTGGTTCGACGCCATTGACAGTATTGATGTTGAAGAGCTGGGTGTCATCCAAGAAAAACCTGTAGATTTTGAGGTTTGTGATGGTATAATACTCCCAGAAAATCAACCTGGTCATATGGTCCAGTTTGAAGATGATGGAAAGAACTACATGTTCTTCCGCTTCAAACGTGAAGACAATGTTTATTATACACCAATGTCACAACTTGGTGCAATTAATGTAGTTTGCAAAGCAGGTGGCAAGACTGTCACGTTTGGTGAAACTACTGTGAAAGAAATACCACCACCTGATGTGGTGCCTATTAAGGTTAGCATCGAGTGTTGTGGTGAACCATGGAATACAATTTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAAACTGACCTCACAGTAGAACAATTGCTCTCTGTGATCTATGAGAAAATGTGTGACGACCTTAAATTGTTTCCTGAGGCTCCAGAACCTCCACCATTTGAGAACGTTGCACTTGTAGATAAGAACGGTAAAGATTTGGATTGCATAAAATCATGCCACCTTGTCTACCGTGATTATGAGAGTGATGATGACATCGAAGAAGAAGATGCTGAGGAATGTGACACTGCCTCAGATGACGCTGAGGAGTGTGACACTGGTTCGGAGTGTGAAGAAGAGGATGAAGGTACTAAGGTGTTGGCTCTTATACAAGACCCAGCAAGTCAAAAATACCCATTGCCTCTAGATGATGATTACAGTGTCTATAATGGGTGTATAGTCCATAAGGATGCTCTTGATGTTGTGAATCTACCATCCGGTGAAGAAACTTTTGTTATCAACAACTGTTTTGAGGGAGCTGTAAAACAACTTCCAAAGTCGGTTGTAGATGTACTTGGTGACTGGGGTGAAGCTGTTGATGCACAGGAGAAGTCCTTGAATGACGTAGCTGATTCCAGTAGTGTTTCGTTGCCGATTGAAGAGCCGGTAATAATTCATGAACAGGAATTTGTTCCTGTTGTAGAAGAAGAGCTAGATGTTATAGTTTGCACTCCAGCTGAATTAAGTGTTGTAAAGGAAGCTCCAGGAGAGGTTGACGAGTTCATCCTTATTCCAAGTGTTTCTAATGGTGAAGAAGTAGTTTCTACTGTGAGTGCGCCACAGCAGGAAGAATCTAGAGAAGAAGTTGTTCCTGATATTTTGCAACAGGAAGAGCCTAAAATTGAAGAGCCTCCTCAAGTTGTAAAAATACAACGTGAGAGGAAAGCTAAGAAGTTCAAAGTTAAGCCAACCACACAAAAAGCTCCTAATTGTTTGGAGTATAAAACTTGTGTGGGCGATTTGACTGTTGTGATAGCCAAAGCATTGGATGAGTTTAAAGAGTTCTGCCTTGTAAACGCCGCAAATGATTATATGTTTCATGGAGGTGGCGTTGCAAAAGCAATTGCAGACTTTTGTGGTCCAGACTTTGTTGAGTATTGTGAAGACTATGTTAAGAAACATGGTCCCCAGAAAAGACTTGTCACACCTTCTTATGTTAAAGGCATTCAATGTGTGAACAATGTTGTAGGCCCACGCCATGCAGACATTGATGTGTTTGGAAAGCTACTTATTGCGTACAAGAATGTCATTGTTGATGGCGTGGTCAATTACGTTGTACCAGTTCTTTCATCTGGAATTTTTGGAGTTGACTTTAAGTTGTCAATAGATGCTATGCGTGCAGCCTTTAAGGATTGCAACATACGTGTTCTTCTATTCTCTCTGAATCAAGATCATATCGATTATTTCGATGCAACTTGTAAACAGAAGACAATTTATCTTACAGAGGATGGTGTTAAATACCGCTCTGGTGTTAAATACCGCTCTGTGATTGTTAAACCTGGCGACTCTTTGAGTCAATTTGGACAGGTTTTTACACGACATAAGACAGTCTTTACTGCAGATGATGTTGAGGATAAAGAAATTCTCTTCATTCCAACTACGGACAAGGCTGTTTTAGATTATTATGGTTTGGATGCGAAAAAGTATGTGATATATTTACATACGCTTTCACAAAAATGGAAAATCCAATGTAAGGACAATTTTATTCAGCTTGAGTGGCGTGATGGAAATTGTTGGATTAATTCAGCAATAGTAATCCTTCAGGCTGCTAAGATTAAGTTTAAAGGTTTTCTTGCAGAAGCATGGGCTAAGCTATTGGGTGGAGATCCTACAGATTTCGTAGCATGGTGCTATGCGAGTTGCAATGCTAATGTTGGTGATTTTTCAGACACCAACTGGCTTTCGGCTAATTTGGCTGAGTATTTTGAAGTGGATTACACGAATGCACTTCTTAAGAAGCGTGTTTCTTGTAATTGTGGTGTTAAGAATTATGAGTTTAGGGGCCTTGAAGCTTGTATTCAACCAGTAAAGGCACCTAACCTGTTACATTTTAAGACACAGTATTCAAACTGTCTCACTTGTGGTTCTAATAGTGTAGATGAAGTGATAGAAGCCTCGCTGCCTTATTTGCTGCTTCTTGCTACAGATGGTCCTGCAGCAGTTGATTGTGATGAAAATGCTGTAGGAAATGTTGTTTTCATTGGGTCTACTAATAGTGGTCATTGTTATACACAAGCTGCTGGAAAAGCTTTTGATAATTTGGCTCAAGATAGGAAATTTGGAAAGAAATCGCCTTACATTACTGCGATGTATACGCGCTTCTCTCTTAAAAGTAAAAACCTTATGCCTATTGCTAAGAAGAGCAAAGGTAAAAATCAAGTAGTAAAAGAAGATGTTTCTAATCTTGCTATTAGTTCAAAAGCCAGTTTTGATAATCTTACTGACTTTGAGCAGTGGTATGATAGTAACATCTATGAGAGTCTTAAAGTGCAGGAAACACCTGACAATCTCTATGAATATGTGTCATTTACAACAAAGGAGGATTCCAAGTTGCCATTGACACTTAAAGTTCGTGGTATTAAATCAGTTGTTGATTTTAAGTCGAAAGATGGCTTTACTTATAAGTTAACACCTGATACTGATGAAAATTCAAAGGCACCCGTTTATTACCCTGTCTTAGACTCTGTTAGTCTTAAGGCAATTTGGGTTGACGGTAGTGCTAACTTTGTTGTTGGACATCCAAATTATAATAGTAGAGCTTTGCGAATTCCCACTTTTTGGGAGTATGCGGAGAGTTTTGTTAAAATGGGTGTAAAAGTTAATGGTGTTACTATGGGCTTATGGCGCGCAGAACACCTTAATAAACCTAATTTGGAAAATCTTTTCAACATCGTTAAGAAAATGATTGTTGGAACTAATATTATTACTACTCAGTGTGGTAGACTAATTGGTAAGACTGCATCATTTATAGCTGATAAAGTAGGAGATGGTGTAGTTCTTAATGTTACTGATAGAATTAAAGGGTGTTTTGGGTTTACTAGGGAGCACTTTGAAAGAAGAGTGTCTCTACAGTTCTTTAAAACCCTCTTTTTCTTCTTTTTCTGTCTTTTGAAGGCTAGTGTTAAGAGTTTACTGTCTAGCTACAAGAGTGTGCTACGTAAAGTGGTATTTACTGCTTTACTTATACTATGGTTTGTCTACACAAGCAATCCAGTTATATTTACTGGAATACGTGTGTTAGATTTCCTACTTGAAGGTTCATTCTGTAGTCCTTATGCAGATTACGGTAAAGAGTCTTTTGATATTTTACGCTATTGTGGAAGTGACTTCACCTGTCGTGTTTGTTTACATGGTAAGGATTCGCTTCATTTGTATAAACATGCCTATAGCGTAAAACAGTTTTACAAAGATGCAGTTAATGGCATCAGTTTTACATGGAATTGGCTTTATATGCTGTTCTTAATATTGTTTGTTAAGCCAGTGGCAGGTTTTGTTATTATTTGCTACTGTATAAGATATTTGGTTTTAAGCACAACTGTATTGCAAACTGGTGTAGGATTTTTAGACTGGTTTGTTCAAACAGTGTTTGCTAACTTCAATTTTATGGGTGCAGGATTTTACTTCTGGCTCTTTTATAAGATATATATACAGGTTCATCATATAATGTACTGTAAGGATATAACATGTGAAGTGTGTAAGAAAGTTGCACGCAGTAATAGACATGAAGTTAGTGTTGTTGTTAATGGGCGTAAACAGCTTGTTCACGTATACACTAATTCTGGTTACACTTTCTGTAAGAAACATAATTGGTATTGTAAGAATTGTGACAAGTATGGTCACCAGAATACATTTATGTCTCCTGAAGTTGCTGGTGAGCTTTCAGAAAAGCTTAAACGCCATGTTAAGCCTACAGCACATGCGTACCACGTAGTGGATGATGCATGCTTAGTTGATGACTTTGTTAATCTGAAGTACAATGCTGCCACCCCAGGTAAAGAAGGTGTGCATTCTGCTGTTAAATGTTTTAGTGTATCTGATTTCTTAAAGAAAGCAGTCTTTCTTAAGGATGCACAAAAATGTGAACAAATAGCTAATGATAGTTTTATAGTTTGTAATACACACAGTGCGCATGCTTTAGAGGAAGCTAAAAATGCTGCCATCTATTATGCGCAATGTCTTTGTAAACCAATTCTTATACTTGACCAGGCTCTTTATGATCAATTGATTGTAGAACCTGTGTCTAAGAGTGTTGTAGACAAAGTGTGTAATATTTTGTCTAATATAATATCTGTAGATTCTGCAGCTTTGAATTATAAGGCGGGCACACTCCGTGATGCCCTCCTTTCTGTCACTAAGGATGAAGAAGCTGTTGATATGGCTATTTTCTGCCATAATAATGACGTTGAATATACTACTGATGGTTTTACCAATGTGGTGCCATCATATGGTATAGACACTGACAAGTTAACACCTCGAGATAGAGGCTTTTTGATAAATGCAGATGCTGCCATTGCTAACTTGAAAGTGAAAAATTCACCGCCAGTAGTGTGGAAGTATTCTGATCTTGTTAAACTGTCTGACAGTTGTCTTAAGTATTTAATATCAGCAACTGTCAAATCAGGAGGTAGATTTTTCATAACTAGGTCTGGTGCTAAACAAGTTATTGCTTGTTATACTCAGAAATTGTTAGTGGAAAAGAAGGCTGGTGGTGTTATTAGTAGCACTGTTAATAGGTTTAAGAGTTGTTGTAAATGGCTCTTAGTGTTTTATTTACTTTTTACAGCGTGTTGTTTGTGTTATTATCAATGGGAAATGAGTAAAGAATTTGCTCACCCCATGTATGATGTTAACTCTACAATGCATGTAGAAGGGTTTAAGGTTATTGACAAGGGTGTACTCAGGGATATTATGTTAGAAGACACTTGTTTCTCTAATAAGTATGTAAATTTTGACTCATTTTGGGGTAAACCTTATGTAAATAGTAGAGACTGTCCTATTGTAATCGCTATTATAGATGGCGCTGGAACAGTAGCTGCTGGTGTTCCTGGTTTTGTACAGTGGGTCATGGATGGTGTAATGTTTACCCATATGGCACAGACTGAGCGAAGACCGTGGTACATTCCTACTTGGTTTAATAGAGAAATTGTTGGTTATACTCATGATTCTATTGTTACAGAAGGTGAGTTTTATACTTCCATAGCATTGTTTGCTTCTAGATGTTTGTATTTAACATCTAGTAACACACCACAGCTTTACTGCTTTAATGGTGATAGCGATGCTCCTGGTGCATTACCGTTTGCAAGTATACTCCCTCATAGAGTATATTTTCAACCTAATGGAGTTAGGCTTATTGTGCCTCAGCAAATTATGCATACACCATACATAGTTAGACTTGTTTCAGATAGCTATTGTAGAGGTAGTGTTTGTGAGTATACTAAACCAGGTTATTGTGTATCACTGAATTCTCAATGGGTTTTGTTTAATGACGAATACACAAGTAAACCAGGTGTGTTTTGTGGTTCTACTGTTAGAGAACTTTTGTTTAATATGGTTAGTACATTCTTTACGGGTGTTAATCCTAATATCTATATGCAATTGGCGACTATGTTCTTAATACTTGTTGCTGTTGTGTTAGTTTTTGCAATGGTTATAAAGTTTCAGGGTGTTTTCAAAGCTTATGCAACCACTGTATTTGTTATAATGCTTGTTTGGCTTATTAACGCATTTGTATTATGTGTACATAGTTATAATAGTGTCTTAGCTATTATACTACTTGTGCTCTATTGTTATGTATCACTTGCGACGAGTCGTAATACTGCCATAGTAATGCACTGTTGGCTTGTTTTCACATTTGGTTTAATAGTACCTAATTGGATAGTTTGCGTCTATTTAGGTTTTATTCTATATATGTACACACCGCTGTTTTTGTGGTGTTATGGTACAACTAAAAATAGTCGCAAGCTTTATGAAGGCAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGTACTTTTGTAGTTCGTGGGCCTGAATTTGTTAAACTTACGAATGAGCTAGGCGATAAATTTGAATCCTATCTCTCAGCGTATGCTAGGCTTAAATACTACTCAGGCACTGGCAGTGAACAAGATTACTTGCAAGCATGCCGTGCATGGTTGGCTTATGCACTAGATCAATTTAGAAGCAGTGGTGTAGAAGTAGTATATACTCCGCCACGGTATTCAATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAACTAGCATCTCCTAGTAGTGCTGTTGAGAAGTGCATTGTTAGTGTGTCCTATAGGGGTAATAATCTTAATGGATTGTGGTTGGGTGATTCTATCTACTGTCCACGTCATGTGTTAGGTAAGTTTAGTGGTGATCAATGGGATGATGTACTTAACTTTGCTAATAATCATGAGTTTGAAGTTATAACTGGAAGGGGTGTTGCTTTGAATGTTGTCAGTAGGCGCTTGAGAGGTGCAGTTTTAATTTTACAAACTGCAATTGCCAATGCTGAAACTCCAAAGTACAAGTTTATGAAAGCAAATTGTGGTGATAGTTTTACCATTGCTTGCTCTTATGGTGGTACAGTTATAGGACTTTACCCTGTCACTATGCGTTCTAATGGAACTATTAGAGCATCTTTTCTTGCTGGAGCATGTGGTTCAGTAGGTTTTAATATAGAAAAGGGTGTAGTAAATTTTTACTACATGCACCATCTAGAGTTACCTAATGCATTACACACTGGAACTGACCTAACAGGTGAGTTTTATGGTGGTTACATAGATGAAGAGGTTGCTCAGAAAGTTCAACCCGATAAATTGGTTACTAATAATATTTTAGCATGGCTTTATGCAGCAATTATTAGTGTTAAAGAGAGTAGTTTTTCAATGCCAAAGTGGCTTGAAAGTACCACCATCAGTATTGATGATTATAATAAGTGGGCAGCGGATAATGGTTTCACATCATTTGTGAGCTGCACTGCTATTACTAAATTAAGTGCTATAACGGGAGTTGATGTTTGTAAACTCCTCCGCACTATTATGGCGAAAAGTGCACAATGGGGTAGTGACCCTATTTTGGGGCAGTATAATTTCGAGGATGAACTGACACCAGAATCTGTTTTTAACCAGGTGGGTGGTGTTAGACTACAATTTTCTGTTGTAAAGAAAGCTGCATCTTGGTTTTGGAGTAGATGTGTGCTAGCTTGCTTCCTCTTTGTCTTGTGTTCTATTGTGCTGTTTACAGCCATACCATATAGATATTATTTACATGGTGCTGTTGTTTTATTCGCAACTGTGCTTTTTATTTCGTTTACTGTGAAACATGTTATGGCATATATGGATACTTTCCTTTTGCCGACATTGATTACAGTTATTATAGGAGTTTGTGCTGAAGTACCTTTCATCTACAATACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTGGTCTTTGACACTGTAGTACCGTGGATGTTCTTACCTTTAGTCTTATATACATCTTTTAAATGTATACAAGGTTGTTATAGTGTAAATTCCTTTAATACTTCTCTGTTATTGTTATATCAGTTTATGAAGTTGGGTTTTGTTATAGTCACTTCTTCTAATACTCTTGCAGCATATTCAGAGGGTAATTGGGAGTTGTTCTTTGAGTTAGTTCATACTACTGTGCTGGCTAATGTTAGTAGCAATTCTTTAATTGGCTTGTTTGTTTTTAAATTGGCTAAGTGGATGTTGTATTATTGTAATGCATCATACCTTAATAATTATGTTCTAATGGCTGTCATTGTTAATGGTATAGGTTGGATGTTTACCTGTTACTTTGGATTTTATTGGTGGATTAATAAGGTTTTTGGTTTAACCTTAGGCAAATATAATTTCAAAGTTTCAGTAGATCAATATAGGTATATGTGTCTCCATAAGATTGACCCGCCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGGATAGGTGGTGAGCGTGTGCTTCCTATAGCCACTGTTCAATCTAAATTAAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATTCTTGCCTCAGACGATGTTAATGAGTGCATGGATAACTTGTTGGGTATGCTTGTAACACTCTTTTGTGTAGATAGTACTATTGATTTAAGTGAATATTGTGATGATATACTTAAGAGATCAACTGTTTTACAGTCAGTTACTAAGGAGTTTTCACATATTCCTTCTTATGCAGAGTATGAAAGAGCTAAAGATCTCTATGAAAAGGTTTTAGCTGAGTCTAAAAACGGTGGTGTTACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAACATTGCAAAGTCAGTTTTTGATAGAGATCTGGCTGTTCAGAAGAAGTTGGATAGCATGGCTGAACGTGCTATGACAACAATGTATAAGGAAGCGCGTGTTACTGATAGAAGGGCGAAACTTGTTTCATCATTACATGCGCTATTATTTTCTATGCTTAAGAAGATAGATTCTGAAAAGCTTAATGTACTATTTGACCAAGCAAGCAGTGGTGTTGTGCCACTAGCTACTGTTCCAATAGTTTGTAGTAATAAGCTTACTCTTGTAGTGCCAGACCCAGAAACATGGGTCAAGTGCGTAGAGGGCATGCATGTTACATATTCAACAGTTGTTTGGAATATTGATAATGTCACTGATGCAGATGGCACTGAATTGCAACCAATTTCTACAGGTAATGGTTTAACCTACTGTATAAGTGGTGATACTATAGCATGGCCCCTTAAGGTCACTTTGACTAGGAATGCGCATAATAAAGTTGATGCAGTATTGCAAAACAACGAGCTTATGCCGCACGGTGTTAAAACAAAAGCTTGCGTTGCAGGTGTGGATCAAGCACATTGCAGCGTAGAGTCTAAGTGTTATTACACTAATATTAGTGGTAATTCAGTTGTTGCTGCTATTACTTCTTCTAATCCTAATTTGAAAGTTGCCTCATTCTTGAATGAAGCAGGCAATCAAATTTATGTTGACTTAGACCCACCATGTAAATTTTGTATGAAGGTGGGTGATAAAGTAGAAGTTGTTTACTTGTATTTCATAAAGAATACAAGGTCAATTGTTAGGGGTATGGTGCTTGGTGCTATATCTAATGTTGTAGTGTTACAGTCTAAAGGATATGAGACAGAGGAAGTAGATTCCGTTGGCATACTATCGCTTTGTTCATTTGCAGTAGACCCAGCTGACACGTATTGTAAGTATGTAGCAGCAGGTAATCAACCTTTAGGTAATTGTGTAAAAATGCTGACAGTTCATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGTCCAACTCCTGATCAGGATTCTTATGGAGGAGCGTCTGTGTGTCTTTATTGTAGAGCACACATAGCACACCCAGGAAGTGCAGGAAATTTAGATGGACGTTGTCAATTTAAAGGTTCTTTTGTGCAAATACCTACTACGGAGAAAGACCCTGTTGGTTTCTGTCTGCGTAATAAGGTTTGCACTGTGTGTCAGTGTTGGATAGGTCATGGTTGTCAGTGTGATGCACTTAGACAACCTAAACCTGCTATTCAAGCAGATGCTGGTGCACCTGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSSQNQRSVVLVAKDIPDQLRDALFFYTSHNPKDYADAFAFKRKFDRNLGAGKQFKFETVCGPFFLKGVDKVTPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVDALNARAQTLEEIFGPTEVLWLQVASKLQVSAMAMRRLVGEVTAKVMDALGSKLSSLFQIVKQQIARIFQKALAIFEDVKDLPQRIAALQMAFAKCAKTITVVVVEKAIVVREFSGTCLASINGAIAKFFAELPNGFMGSKVFSTLAFFKEAAVKIVENVPGAPQGTKGFEVVGNAKGTQVVIRGMRNDLTLLDQKAEIPVEPEGWSAIMDGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLVDGVTPEINDGLILAAIYSSFSVSELVAALKKGEPFKFLGHKFVYAKEAAVSFTLAKAATISDVLKLFQSARVETEDVWSALTEKSFEFWKLAYGKVRNLEEFVKTHFCKAQMSIVVLAVVLGEGIWHLVSQVIYRLGCSFSKVIDFCAKYWKGFCGQLKKAKLLVTEHLCVLKGVAQHCFQLLLDAIHSMYKSFQKCALGRIHGDLLFWKGGVHKVVQEGDEIWFDAIDSIDVEELGVIQEKPVDFEVCDGIILPENQPGHMVQFEDDGKNYMFFRFKREDNVYYTPMSQLGAINVVCKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYEKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLVYRDYESDDDIEEEDAEECDTASDDAEECDTGSECEEEDEGTKVLALIQDPASQKYPLPLDDDYSVYNGCIVHKDALDVVNLPSGEETFVINNCFEGAVKQLPKSVVDVLGDWGEAVDAQEKSLNDVADSSSVSLPIEEPVIIHEQEFVPVVEEELDVIVCTPAELSVVKEAPGEVDEFILIPSVSNGEEVVSTVSAPQQEESREEVVPDILQQEEPKIEEPPQVVKIQRERKAKKFKVKPTTQKAPNCLEYKTCVGDLTVVIAKALDEFKEFCLVNAANDYMFHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQKRLVTPSYVKGIQCVNNVVGPRHADIDVFGKLLIAYKNVIVDGVVNYVVPVLSSGIFGVDFKLSIDAMRAAFKDCNIRVLLFSLNQDHIDYFDATCKQKTIYLTEDGVKYRSGVKYRSVIVKPGDSLSQFGQVFTRHKTVFTADDVEDKEILFIPTTDKAVLDYYGLDAKKYVIYLHTLSQKWKIQCKDNFIQLEWRDGNCWINSAIVILQAAKIKFKGFLAEAWAKLLGGDPTDFVAWCYASCNANVGDFSDTNWLSANLAEYFEVDYTNALLKKRVSCNCGVKNYEFRGLEACIQPVKAPNLLHFKTQYSNCLTCGSNSVDEVIEASLPYLLLLATDGPAAVDCDENAVGNVVFIGSTNSGHCYTQAAGKAFDNLAQDRKFGKKSPYITAMYTRFSLKSKNLMPIAKKSKGKNQVVKEDVSNLAISSKASFDNLTDFEQWYDSNIYESLKVQETPDNLYEYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLTPDTDENSKAPVYYPVLDSVSLKAIWVDGSANFVVGHPNYNSRALRIPTFWEYAESFVKMGVKVNGVTMGLWRAEHLNKPNLENLFNIVKKMIVGTNIITTQCGRLIGKTASFIADKVGDGVVLNVTDRIKGCFGFTREHFERRVSLQFFKTLFFFFFCLLKASVKSLLSSYKSVLRKVVFTALLILWFVYTSNPVIFTGIRVLDFLLEGSFCSPYADYGKESFDILRYCGSDFTCRVCLHGKDSLHLYKHAYSVKQFYKDAVNGISFTWNWLYMLFLILFVKPVAGFVIICYCIRYLVLSTTVLQTGVGFLDWFVQTVFANFNFMGAGFYFWLFYKIYIQVHHIMYCKDITCEVCKKVARSNRHEVSVVVNGRKQLVHVYTNSGYTFCKKHNWYCKNCDKYGHQNTFMSPEVAGELSEKLKRHVKPTAHAYHVVDDACLVDDFVNLKYNAATPGKEGVHSAVKCFSVSDFLKKAVFLKDAQKCEQIANDSFIVCNTHSAHALEEAKNAAIYYAQCLCKPILILDQALYDQLIVEPVSKSVVDKVCNILSNIISVDSAALNYKAGTLRDALLSVTKDEEAVDMAIFCHNNDVEYTTDGFTNVVPSYGIDTDKLTPRDRGFLINADAAIANLKVKNSPPVVWKYSDLVKLSDSCLKYLISATVKSGGRFFITRSGAKQVIACYTQKLLVEKKAGGVISSTVNRFKSCCKWLLVFYLLFTACCLCYYQWEMSKEFAHPMYDVNSTMHVEGFKVIDKGVLRDIMLEDTCFSNKYVNFDSFWGKPYVNSRDCPIVIAIIDGAGTVAAGVPGFVQWVMDGVMFTHMAQTERRPWYIPTWFNREIVGYTHDSIVTEGEFYTSIALFASRCLYLTSSNTPQLYCFNGDSDAPGALPFASILPHRVYFQPNGVRLIVPQQIMHTPYIVRLVSDSYCRGSVCEYTKPGYCVSLNSQWVLFNDEYTSKPGVFCGSTVRELLFNMVSTFFTGVNPNIYMQLATMFLILVAVVLVFAMVIKFQGVFKAYATTVFVIMLVWLINAFVLCVHSYNSVLAIILLVLYCYVSLATSRNTAIVMHCWLVFTFGLIVPNWIVCVYLGFILYMYTPLFLWCYGTTKNSRKLYEGNEFVGNYDLAAKSTFVVRGPEFVKLTNELGDKFESYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQFRSSGVEVVYTPPRYSIGVSRLQAGFKKLASPSSAVEKCIVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWDDVLNFANNHEFEVITGRGVALNVVSRRLRGAVLILQTAIANAETPKYKFMKANCGDSFTIACSYGGTVIGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLTGEFYGGYIDEEVAQKVQPDKLVTNNILAWLYAAIISVKESSFSMPKWLESTTISIDDYNKWAADNGFTSFVSCTAITKLSAITGVDVCKLLRTIMAKSAQWGSDPILGQYNFEDELTPESVFNQVGGVRLQFSVVKKAASWFWSRCVLACFLFVLCSIVLFTAIPYRYYLHGAVVLFATVLFISFTVKHVMAYMDTFLLPTLITVIIGVCAEVPFIYNTLISQIVIFFSQWYDPVVFDTVVPWMFLPLVLYTSFKCIQGCYSVNSFNTSLLLLYQFMKLGFVIVTSSNTLAAYSEGNWELFFELVHTTVLANVSSNSLIGLFVFKLAKWMLYYCNASYLNNYVLMAVIVNGIGWMFTCYFGFYWWINKVFGLTLGKYNFKVSVDQYRYMCLHKIDPPKTVWEVFSTNILIQGIGGERVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVNECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTKEFSHIPSYAEYERAKDLYEKVLAESKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWVKCVEGMHVTYSTVVWNIDNVTDADGTELQPISTGNGLTYCISGDTIAWPLKVTLTRNAHNKVDAVLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFCMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGYETEEVDSVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGHGCQCDALRQPKPAIQADAGAPGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 616 Identity=85.20%
Cov(Q)=9.02% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3969
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
423.207259999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00572
outside
1 - 1741
TMhelix
1742 - 1761
inside
1762 - 1767
TMhelix
1768 - 1790
outside
1791 - 1850
TMhelix
1851 - 1870
inside
1871 - 1876
TMhelix
1877 - 1899
outside
1900 - 1902
TMhelix
1903 - 1925
inside
1926 - 2297
TMhelix
2298 - 2317
outside
2318 - 2378
TMhelix
2379 - 2401
inside
2402 - 2574
TMhelix
2575 - 2597
outside
2598 - 2601
TMhelix
2602 - 2624
inside
2625 - 2630
TMhelix
2631 - 2653
outside
2654 - 2667
TMhelix
2668 - 2690
inside
2691 - 3112
TMhelix
3113 - 3135
outside
3136 - 3139
TMhelix
3140 - 3157
inside
3158 - 3169
TMhelix
3170 - 3192
outside
3193 - 3206
TMhelix
3207 - 3229
inside
3230 - 3241
TMhelix
3242 - 3264
outside
3265 - 3273
TMhelix
3274 - 3296
inside
3297 - 3315
TMhelix
3316 - 3338
outside
3339 - 3969
