Strain
Canine_JN856008
(Region: USA: Ithaca, NY; Strain: Canine coronavirus strain A76, complete genome.; Date: 1976)
Gene
nucleocapsid protein
Description
Annotated in NCBI,
nucleocapsid protein
Location
GenBank Accession
Full name
Nucleoprotein
Alternative Name
Nucleocapsid protein
Sequence
CDS
ATGGCCAACCAGGGACAACGCGTTAGTTGGGGAGATGAATCTACCAAAAAACGTGGCCGTTCCAATTCTCGTGGTCGGAAGAATAATAATATACCTCTTTCATTCTTCAATCCCATTACCCTCCAACAAGGTGCAAAATTTTGGAATTTATGTCCGAAAGACTTTGTACCCAAAGGAATAGGTAACAGGGATCAACAAATTGGTTATTGGAATAGACAAACTCGCTATCGCATGGTGAAGGGCCAACGTAAGGAGCTTCCTGAAAGATGGTTCTTCTACTATTTGGGCACTGGACCTCATGCAGATGCCAAATTTAAAGATAGAATAGATGGAGTTGTCTGGGTTGCCAAGGATGGTGCCATGAATAAACCAACTACACTTGGTAATCGTGGTGCTAATAATGAATCCAAAGCTTTGAAATTCGATGGTAAAGTGCCAGGCGAATTCCAACTTGAAGTGAACCAATCAAGAGACAATTCAAGGTCACGCTCTCAATCTAGATCTCAGTCTAGAAATAGATCTCAATCTAGAGGAAGGCAACAATCCAATAACAAGAAGGATGACAGTGTAGAACAAGCTGTTCTTGCTGCACTTAAAAAGTTAGGTGTTGACACAGAAAAACAACAACAACGCTCTCGTTCTAAATCTAAAGAACGTAGCAACTCTAAGACAAGAGACACTACACCTAAGAATGATAACAAACACACCTGGAAGAGAACTGCAGGTAAAGGTGATGTGACAAAATTTTATGGAGCTAGAAGTAGTTCAGCCAATTTTGGTGACAGCGATCTCGTTGCCAATGGGAGCGGTGCCAAGCATTACCCACAACTGGCTGAATGTGTTCCATCTGTATCTAGCATTCTGTTTGGAAGCTATTGGACTGCAAAGGAAGATGGCGACCAGATAGAAGTCACATTCACACACAAATACCACTTGCCAAAGGATGATCCTAAGACTGGACAATTCCTTCAGCAGATTAATGCTTATGCTCGTCCATCAGAAGTGGCAAAAGAACAGAGACAAAGAAAGGCTCGTTCTAAATCTGCAGAAAGGTCAGAGCAAGAGGTTGTACCTGATGCATTAACAGAAAATTATACAGATGTGTTTGATGACACACAGGTTGAGATTATTGATGAGGTAACGAACTAA
Protein
MANQGQRVSWGDESTKKRGRSNSRGRKNNNIPLSFFNPITLQQGAKFWNLCPKDFVPKGIGNRDQQIGYWNRQTRYRMVKGQRKELPERWFFYYLGTGPHADAKFKDRIDGVVWVAKDGAMNKPTTLGNRGANNESKALKFDGKVPGEFQLEVNQSRDNSRSRSQSRSQSRNRSQSRGRQQSNNKKDDSVEQAVLAALKKLGVDTEKQQQRSRSKSKERSNSKTRDTTPKNDNKHTWKRTAGKGDVTKFYGARSSSANFGDSDLVANGSGAKHYPQLAECVPSVSSILFGSYWTAKEDGDQIEVTFTHKYHLPKDDPKTGQFLQQINAYARPSEVAKEQRQRKARSKSAERSEQEVVPDALTENYTDVFDDTQVEIIDEVTN
Summary
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Subunit
Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
Similarity
Belongs to the alphacoronavirus nucleocapsid protein family.
Keywords
ADP-ribosylation
Host Golgi apparatus
Phosphoprotein
Ribonucleoprotein
RNA-binding
Transcription
Transcription regulation
Viral nucleoprotein
Virion
Feature
chain Nucleoprotein
Uniprot
Pubmed
EMBL
PRIDE
Pfam
PF00937
Corona_nucleoca
Interpro
ProteinModelPortal
PDB
5N4K
E-value=9e-35 Score= 125 Identity=49.17%
Cov(Q)=31.41% Cov(P)=80.00%
3D Structure
Ontologies
GO
Subcellular Location
From MSLVP
Host nucleus > host nucleolus. Host cytoplasm. Secreted.
From Uniprot
Virion
Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host Golgi apparatus Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host Golgi apparatus Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Topology
Length:
382
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.0133
Exp number, first 60 AAs:
0.01115
Total prob of N-in:
0.02789
outside
1 - 382
