Strain
Avian_KF931628
(Region: Australia; Strain: Infectious bronchitis virus isolate VicS-del, complete genome.; Date: 6-Aug-12)
Gene
ORF1a polyprotein
Description
Annotated in NCBI,
ORF1a polyprotein
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTACAAGCCTAAAACAGGGAGTATCTTCCCAAAACCAGAGGAGTGTCATTCTTGTTGCCAAAGACATTCCCGACCAACTTTGTGACGCCTTGTTCTTCTATACGTCACACAAACCTAAGGATTACGCTGATGCTCTTGCCCTTAGGCAGAAGTTTGACCGTAATCTTCAGACTGGGAAACAGTTTAAATTTGAAACTGTGTGTGGTCCCTTCCTCTTGAAGGGAGTCGAAAAAATAACACCTGGTGTCCCAGCAAAAGTTTTGAAAGCCACTTCTAAGTTGGCTGATTTGGAAGACATCTTTGGTGTCTCACCTCTAGCGCGGAAGTACCGTGAATTGTTGAAAACAGCATGTCAGTGGTCTCTCACTGTAGAAACACTGGATACTCGTGCCCAAACTCTTGAAGAGATCTTTGACGCAACTGAAATACTTTGGCTTCAGGTGGCAGCAAAGCTACAGGTATCAGCCATGGCAATGCGCAGACTTGTTGGAGAAGTAACTGTAAAAGTCATGGAGGCACTTGGCTCGAATTTGAGCGTTCTTTTTCAAATTGTTAAAGAACAAATAGCCAGAATCTTTCAAAAGGCGCTGGCTATTTTTGAGGATGTTAGTGAGTTGCCACAGCGTATTTTTGCACTTAAAATGGCTTTTGCCAAGTGTGCAAAATCAATTACAGTTGTGGTTATTGGACATGCTCTTATGATTAAGGAGTTTGCTGGAACTTGCCTTGCAAGCATTAATGGTGCTGTTTCAAAATTCTTTGAAGAACTTCCAAATGGATTCATGGGAGCCAAAATCTTTTCAACGCTTGCTTTCTTTAAGGAGGCTGCGGCAAAGATTGTGGACAATATACCAAACGCACCAAGAGGGACTAAAGGTTTTGAAGTTGTTGGCAATGCCAAGGGTACACAGGTTGTTGTGCGTGGCATGCGGAACGACTTAACACTGCTAGACCAAAAAGCTGATATTCCCGTAGAACCAGAAGGCTGGTCGGCAATTTTGGATGGACATCTTTGTTATGTCTTTAGGAGTGGCGATAGGTTTTATGCAGCGCCCTTGTCAGGAAATTTTGCACTTCATGATGTGTATTGCTGTGAGCGTGTTGTCTGTCTAGTTGATGGTGTAACACCAGAGATAAATGATGGACTCATTCTTGCTGCAATCTATTCATCTTTTAGTGTAACTGAACTTTTGGCTGCTCTTAAAAAGGGTGAACCATTTAAGTTCCTTGGACACAAATTTGTGTATGTGAAAGAAGCAGCGGTTTCTTTTACTTTGGCAAAGGCAGCCACTATAACTGATGTCTTGAAGCTGTTTCAATCAGCGAGAGTAAAGACAGAAGATGTGTGTTCTGCATTTACTGAGAGGTCTTTCGAATTCTGGAAACTCGCATATGGAAAAGTGAGGAACCTTGAAGAATTTGTGAAAACTCATTTCTGTAAAGCTCAAATGTCAATTGTAATTCTAGCAGCAGTGCTTGGTGAAGGCATTTGGCAGATTGTTTCACAAATCATTTATAAATTGGGCGGTTTGTTTTCTAAAGTTGTTGACTTCTGTGAAAAACATTGGAAAGGTTTCTGTGGACAATTGAAAAGGGCTAAGCTCCTTGTTACAGAACAGCTTTGTGTCTTAAAGGGTGTTGCACAGCATTGTTTTCAACTTTTGCTTGACGCAATACATTCTTGTTATAAGAGTTTTAAGAAGTGTGCACTTGGTAGAATCCATGGAGATTTACTCTTCTGGAGAGGTGTACACAAAATTGTCCAGGATGGTGATGAAATCTGGTTTGACGCCATTGATAGTATTGATGTTGAAGATCTGGGTGTTGTACAAGACAAGCCTGTGGATTTTGAGGTTTGCGATGATGTAACACTGCCAGAAAATCAACCTGGTCACATGGTTCAGTTTCAACATGACGGAAATAACTATATGTTTTTTCGTTTCAAGAAGGATGAGAACATCTATTATACACCAATGTCACAACTTGGTGCAATTAATGTAGTTTGCAAAGCAGGCGGCAAGACTGTCACTTTCGGTGAAACTACTGTGAAAGAAATACCACCGCCTGATGTTGTGCCTATTAAGGTTAGCATCGAGTGTTGTGGTGAACCATGGAATACTATCTTCAAAAAGGCTTATAAGGAGCCCATTGAAGTAGAGATAGATCTCACAGTAGAACAATTGCTCTCTGTGATCTATCAGAAAATGTGTGATGATCTTAAACTGTTTCCAGAGGCTCCAGAGCCTCCACCTTTTGAGAATGTCGCACTTGTTGATAAGAACGGTAAGGATTTGGATTGTATAAAGTCATGCCATCTGATCTACCGTGATTGTGAGAGCGATGATGACATCGAAGAAGAAGATGCTGAGGAGTGTGACACAGGCTCAGATGATGCTGAGGAGTGTGATACTGCTTCCGAGTGTGAAGAAGAGGATGAGGATACTAAAGTGTTGGCGCTTATACAGGACCCAGCAAGTACTAAATACCCTCTTCCTCTGGATGAAGATTATAGCGTTTATAATGGATGTATTGTTCATAGAGACGCTCTTGATGTTGTGAATTTACCATCTGGTGAAGAAACCTTCGTTGTCAATAACTGTTTTGATGGCGCTGTTAAACCTCTGCCCCAGAAAGTTATTGATGTTCTTGGAGATTGGGGAGAGGCTGTTGACGCGCAAGAAAAATTGTGTGAACAAGAGCCTGAACCTTTTAAAGAACCAGTTGAACAATCTGCTGATGTTGATGGTGGAGCGACTAAACAAGTTGTTGCTGTAGAGCAGGAAGTTATTCCTGTTGTTGACGAGTCTCAAGAGGTAGTTGTTTATACATCTGCTGATTTAGAGGTTGTTAAAGAAACATCCGAAGATGTTGATGAGTTTATTCTTGTTTCTGATGTTTCTACAGAAGAAATTGTGTCTCAAGAAGGAAAAGAGCCACAAGTGGAACTAGAGCCCATTCAAGTTGTCAAACCACAACGTGAGAAGAAGGCTAAGAAGTTTAAAGTCAGACAAACCACATGTAAAGAGCCAAAATTTTTGGAGTATAAAACATGTGTAGGTGATTTGACTGTGGTAATTGCCAAAGCATTGGATGAATTTAAAGAGTTCTGCGTTGTAAACGCCGCAAATGAGCATATGTCTCATGGTGGTGGTGTTGCAAAGGCAATTGCAGACTTTTGTGGACCAGATTTTGTGGAGTATTGTGAGGATTATGTAAAGAAACATGGCCCTCAACAACGACTTGTCACGCCTTCGTATGTTAAAGGCATTCAATGTGTGAATAATGTAGTAGGACCGCGCCACGGAGATAAAGACTTGGAAGAAAAGCTTGTCTTTGCATACAAGAATGTACTTGTAGATGGTGTGGTCAATTATGTGGTGCCAGTCCTCTCGTCAGGAATTTTTGGTGTTGATTTTAAATTATCTATAGACGCGATGCGTAAAGCCTTTAAAGGGCTTAACATACGCGTTCTTCTTTTTTCTCTGAGTCAAGAACACATCGATTATTTCGATGCAACTTGTAAACAGAAGAAAATTTATCTTACAGAGGATGGTGTAAAATACCGCTCTGTTATTGTTAAACCTGGAGACTCTCTGAGTCAGTTTGGACAGGTTTTTGCAAAGAATAAGACAGTTTTTACTGCAGATGATGTTGAGGACGAAGAAGTTCTTTTCATCCCAACAACTGATAAAGCTGTTCTTGAATATTATGGTTTGGATGCGAAAAAGTATGTGATATATTTACAAACACTTTCGCAGAGATGGAAAATCCTGCATAAGGACAATTTTATCCAACTTGAGTGGCGTGATGGAAATTGCTGGATTAATGCTGCAATAGTATTACTTCAAGCTGCCAATATTAGGTTTAGAGGTTTTCTTGAAGAAGCATGGGCTAAGCTTGTGGGTGGAGATCCCACAGATTTTGTGGCCTGGTGCTATGCAAGTTGCAATGCTAATGTTGGTGAGTTTTCAGATGCTAATTGGCTTATAGCTAATTTGGCAGAATATTTTGAAGTGGATTACACGAATGCACTTCTTAAGAAGCGTGTGTCCTGTAATTGCGGTGTTAAGAACTATGAACTTAGGGGCATTGAAGCGTGTATTCAACCAGTCAGGGCTCCTAACCTCTTACATTTTAAGACGCAATATTCAAATTGCCCTACTTGTGGTTCAAATAGTGTAGACGAGGTTGTTGAAGCTTCGCTGCCTTATTTGTTATTACTTGCTACTGATGGCCCAGTCTCAGTTGATTGTGATGAGAATGTTGCCGGTAATGTAGTTTTTATTGGGTCCACTAATAGTGGCCATTGCTATACACAAGCTGTTGGAAAAGCTTTTGATAATTTGGCTCAAGATAGGAAATTCGGAAAGAGGTCGCCCTACATTACAGCAATGTATACACGATTCTCTCTTAAGAGTGAAAACTCTTCATCTGTTGCTAAAAAGGGTGAAGGTGAGTTGGTAAAAGTAGATGTTTCTAGCTTAGCTATTAGTTCCAAAGTTAGTTTTGATAATCTTACTGACTTTGAACAGTGGTATGATAGTAACATTTATGAAAGTCTTAAAGTGCAGGAAACACCTGACAACATGGACGATTATGTGTCATTTACAACAAAGGAAGATTCTAAGTTGCCACTGACACTTAAAGTTAGAGGTATTAAATCAGTTGTTGACTTTAAGTCAAAAGATGGTTTTACTTACAAGTTAATACCTGATAATGATGAGAATTCAAAAGCACCAGTCTACTACCCCGTCTTGGACTCTATTAGTCTTAAGGCAATATGGATAGAAGGCAGTGCTAATTTTGTTGTTGGACATCCAAATTATAATAGTAGAGCTCTGCGAATTCCTACTTTTTGGGAGTTTGCAGAGAATTTTGTAAAGATAGGTGAAAAAGTTGATGGTGTAACTATGGGCCTATGGCGTGCAGAAAACCTTAATAAACCTAATTTGGAGAGAATTTTCAACATCATTAAGAAAATGATTGTTGGATCTAGTGTCGTTACTACTCAATGTGGTAAGTTAATTAGTAAAGCGACTACATTCATTGCTGATAAAGTAGGTGATGGCGTGGTCCGCAATATCACAGATAGAATTAAGGGTTGTTTTGGAATGACACGTGGTCATTTTGAAAGAAAAATTTCACCACAATTCCTAAAAACACTTATGTTCTTCTTCTTTTATTTAGTTAAGACTAGCGCTAAGAGTTTAGTTGCTAGCTATAAGATTATTTTATGTAAGGTAGTTTTTACTGCTTTACTTATATTATGGTTTGTTTACACAAGTGATCCAGTAATGTTTACAGGAATACGTGTGTTAGACTTCCTATTTGAAGGCTCTTTATGTGGTCCTTATAGTGGCTATGGTAAGGAGTCTTTTGATGTGCTACGCTATTGTGCAGATGATTATACTTGTCGTGTTTGTTTACATGGCAGAGATTCACTTCATTTGTATAAACATGCATATAGCGTAGAACAGTTCTACAAAGCTGCAGTTAATGGCATTAGTTTTAATTGGAATTGGCTTTATATGCTCTTTCTAATATTGTTTGTTAAGCCAGTAGCAGGTTTTGTTATTATTTGCTATTGTGTTAAGTATTTGGTATTGAATTCAACTGTGCTGCAAACTGGTGTAAGTTTCCTAGACTGGTTTGTCCAAACAGTGTTTGCAAATTTTAATTTTATGGGCGCAGGATTTTATTTCTGGCTATTTTACAAAATATATATACAGGTGCATCATATACTGTACTGTAAGGATATAACATGTGATGTGTGTAAGAGGGTTGCACGCAGTAATAGGTATGAGGTTAGTGTTGTTGTTAGTGGGCGTAAACAGCTTGTTCACGTATATACTAACTCTGGTTACAATTTTTGTAAGAGACATAACTGGTACTGTAAGGATTGTGATAAGTATGGTCATCAAAACACTTTTATGTCTCCCGAAGTTGCAGGCGAGCTTTCTGAAAAGCTTAAGCGTCATGTTAGACCTACAGCATTTGCATACCACGTAGTGGATGATGCATGCGTAGTTGATGGCTTTGTTAATATGAAGTATAATGCTGCTACTCCAGGTAAAGATGGTTTGCATGCTGCTGTTAAGTGTTTTAGTGTATCAGATTTTTTAAAGAAATCTGTCTTTCTTAAGGATGCACAAAAATGTGATCAAATATCCAATGATAGTTTTATAGTTTGTAATACACAGAGTGCACATGCATTAGAGGAAGCGAAAAATGCAGCCATTTATTATGCGCAATGCCTTTGTAAACCAATCCTTATACTTGACCAGGCTCTTTATGAACGATTGATTGTAGAACCTGTGTCTAAGAGTGTTGTAGACAAAGTTTGTAGCATTTTGTCTAGTATTGTATCTGTAGATACTGCAGCTCTGAATTATAAGGCAGGCACACTTCGTGATGCTCTCCTTTCTGTTACTGAAGATGAAGAAGCTGTAGATATGGCTATCTTCTGTCACAATAATGATGTAGAGTGTACTAGTGATGGATTTACTAATGTGGTTCCATCATATGGTATAGATACTAATAAATTAACACCTCGTGATAGAGGCTTTTTAATAAATGCAGATGCTTCCATTGCTAACTTGAAAGTGAAAAATGCACCGCCAGTAGTGTGGAAGTTTTCTGACCTTGTTAAATTGTCTGACAGTTGTCTAAAGTATTTAATATCAGCAACTGTTAAGTCAGGAGGTAGGTTTCTCATAACAAGGTCTGGTGCTAAACAGGTTATTTCTTGTTATACACAGAAATTGTTAGTTGAGAAAAAGGCTGGTGGTGTTGTTAGCAGCACTGTTGGTGCTTTCAAGAGTTGTTGTAAATGGCTCTTGGTTTCTTATTTCCTTCTTACATTGTGTTGTTTGGGTTATTATCAGTGGGAAATGAGTAGAGATTTTGCTCAACCCATGTATGATTTTAATACTACAATGCATGTTGAAGGTTTTAAGGTTATAGATAAAGGTGTTGTCAGGGACATTGTACCAGAAGACACCTGTTTCTCTAATAAATTTGTTAATTTTGATGCCTTTTGGGGTAAACCATATGACAATAGCAGAGATTGTCCAATAGTAACTGCCATTATAGATGGTGTAGGAACAATAGCTGCTGGTGTTCCTGGTTTCGTAGGTTGGGTCATGGATGGTGTAATGTTTATACACATGACACAAACTGAGCGAAAACCTTGGTATGTCCCTACGTGGTTTAATAGGGAAATTGTTGGCTATACTCAGGATTCAATTATTACTGAGGGTAGTTTTTACACATCCATAGCACTTTTTTCAGCTAGATGTTTATATTTAACTGCCAGCAATACACCTCAATTGTATTGTTTTAATGGTGTTAATGATGCTCCTGGAGCCTTACCATTTAGTAGTATAACTCCCCATAGGGTTTATTTTCAACCAAATGGTGTTAGGCTTATAGTTCCACAGCAAATAATGCACACACCCTATATAGTAAAGTTAGTGTCAGACAGCTATTGTAGAGGTAGTGTTTGTGAGTATACTAAACCGGGATACTGTGTGTCAATGAATTCCCAATGGGTTTTGTTCAATGGCGACTACACAAGTAAACCAGGTGTGTTTTGTGGCTCTACTGTTAGGGAACTGTTGTTTAATATGGTTAGCACATTTTTTACTGGCGTTAACCCCAATATTTATATGCAGCTGGCGACTATGTTTTTAATACTAGTTGTTGTTGTTTTAATTTTTGCAATGGTCATAAAGTTTCAAGGTGTTTTTAAAGCTTATGCAACCATTGTTTTTACAATTATGTTAGTTTGGGTTATTAATGCATTTGTATTATGTGTACATAGTTATAACAGTGTTTTAGCTGTTATACTACTTGTACTCTATTGCTATGCATCATTGGTTACAAGTCGTAACACGTCTATAATAATGCATTGTTGGCTTATTTTTACTTTTGGTTTGATAGTACCTACATGGTTGGCCTGTTGCTACCTAGGATTCGTGCTTTATGTTTATACACCATTGTTTTTCTGGTGTTATGGTACCACAAAAAACTCTCGTAAGCTTTATGAGGGTAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGTACTTTTGTAATTCGTGGCTCTGAATTTGTAAAGCTTACGAATGAGCTAGGTGATAAATTTGAAACCTACCTTTCGGCTTATGCTAGACTTAAGTACTATTCAGGCACAGGCAGTGAACAAGATTATTTACAAGCTTGTCGTGCATGGTTGGCTTATGCGCTAGACCAATATAGAAATAGTGGTGTTGAGGTTGTCTATACCCCGCCACGCTATTCTATTGGTGTTAGTAGATTGCAAGCTGGTTTTAAGAAACTGGTTGCGCCTAGTAGTGCTGTAGAAAAGTGCATTGTTAGTGTTTCTTATAGAGGCAATAATCTTAATGGACTGTGGTTGGGTGACTCTATCTACTGTCCACGACATGTCTTAGGTAAGTTTAGTGGTGACCAATGGGATGATGTACTTAACCTTGCTAATAATCATGAGTTTGAAGTTACAGCTCAGAATGGTGGTACTTTGAATGTTGTCAGCAGGAGGCTAAAAGGAGCAGTTTTGATTTTACAGACTGCTGTTGCCAATGCTGGAACTCCGAAGTATAAGTTTGTAAAGGCTAATTGTGGTGATAGTTTCACTATAGCTTGCTCTTATGGTGGTATAGTTGTTGGTCTATACCCTGTTACTATGCGCTCTAATGGTACTATTAGGGCTTCTTTTCTTGCTGGAGCTTGTGGTTCAGTAGGTTTTAATATAGAAAAGGGTGTAGTTAATTTTTACTATATGCATCATCTCGAGTTGCCTAATGCCCTACACACTGGAACAGACCTGTGTGGTGAGTTCTATGGTGGTTATGTAGATGAAGAGGTTGCACAAAAAGTGCCACCAGATAACCTAGTTACTAATAATGTTGTTGCATGGTTATATGCTGCAATCATTAGTGTTAAAGAGAGTAGTTTTTCACTGCCAAAATGGCTGGATAGTACTACTGTTAGTGTTGATGATTATAATAAGTGGGCTGGAGACAATGGTTTTACACCGTTTTCTACTAGCACTGCTATTACTAAATTGAGTGCTATAACGGGAGTCGATGTTTGTAAACTCCTACGCACTATTATGGTTAAGGCTAGTCAATGGGGCTGTGATCCCATTTTAGGCCAATATAATTTTGAGGATGAGTTAACACCTGAATCTGTGTTTAACCAGGTTGGTGGTGTTAGATTACAATCTTCTTTTATAAGGAAAGCCACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGCTTCTTGTTTGTATTGTGTGCTATTGTTCTATTTACGGCAGTGCCACTTAAATATTATGTGCACGCTGCTGCTATTTTAATGGTAGCCGTGCTTTTTATTTCTTTTACTGTAAAACATGTTATGGCTTATATGGATACTTTCCTATTGCCGACATTAGTTACAGTTATTATTGGAGTTTGTGCTGAAGTACCTTTCATTTACAATACTCTAATTAGTCAAGTTGTTATTTTCTTAAGTCAATGGTATGATCCAGTGGTCTTTGATACTATGGTGCCATGGATGTTTTTGCCATTAGTGTTGTATACAGCTTTTAAGTGTATACAGGGTTGCTATAGTGTTAATTCTCTCAACACTTCGCTGTTAGTGCTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTACACCTCTTCTAATACGCTTACTGCATATACAGAAGGTAATTGGGAGTTATTCTTTGAGTTAGTACACACTACTGTGTTGGCTAATGTTAGTAGTAATTCTTTAATTGGTTTACTTGCGTTTAAGTGTGTTAAGTGGATGTTGTATTATTGTAATGCAACATACGTAAACAACTATGTTTTAATGGCAGTCATGGTTAATGGCATGGGCTGGCTTTGTACTTGTTATTTTGGACTTTATTGGTGGGTTAATAAGGTTTTTGGTTTAACCTTAGGTAAATACAATTTTAAAGTCTCAGTAGATCAATATAGGTATATGTGTTTGCATAAGATAGATTCACCTAAAACTGTGTGGGAATCTTTGCGACAAATATACTTATACAAGGAATTGGGTGATCGTGTATTGCCTATTTCTACAGTTCAGTCTAAATTGAGTGATGTAAAGTGCTCAACTGTTGTCTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAACTCAAAAATGCATGCTTATCTTGTTGATTTACACAATAAAATTCTTGCTTCTGATGATGTTGGAGAATGCATGGATAATTTATTGGGTATGCTTGTTACACTATTTTGTGTTGATAGTACTATTGATTTAAGTGAGTATTGTGATGACATACTTAAGAGATCAACTGTTTTACAATCAGTTACTCAAGAGTTTTCACACATACCCTCTTATGCTGAATATGAAAGAGCTAAAAACCTTTATGAAAAGGTGTTAGCTGATTCTAAAAATGGTGGTGTAACACAGCAAGAACTTGCTGCGTATCGTAAAGCTGCCAATATTGCAAAATCAATTTTTGATAGAGATTTGGCCGTTCAGAAGAAGTTAGATAGCATGGCAGAGCGTGCTATGACAACAATGTACAAAGAGGCGCGTGTAACTGATAGAAGAGCAAAATTAGTTTCGTCACTACACGCTTTACTTTTTTCAATGCTTAAGAAAATAGATTCTGAAAAGCTTAATGCCTTATTTGATCAGGCGAGCAGTGGTGTAGTACCGCTAGCGACTGTTCCAATTGTTTGTAGTAATAAGCTTACACTTGTTATACCAGACCTAGAAACGTGGGTTAAATGCGTAGAAGGTATGCATGTTACATATTCAACAGTTGTCTGGAACATAGATACTGTTATAGATGCCGATGGCATAGAATTGCACCCAACTTCTACAGGTAGTGGATTGACTTACTGTGTAAGTGGTGATAATATATCATGGCCTTTGAAGGTCAACTTGACTAGAAATGTGCATAACAGGGTTGAAGCAGCATTGCAGAACAATGAGCTTATGCCGCATGGTGTTAAAACAAAAGCTTGCGTTGCTGGTGTAGATCAAGCACACTGTAGCGTTGAGTCTAAATGCTATTATACAAATATTAGTGGTAGTTCAGTTGTAGCTGCTATTACCTCTTCCAATCCAAATTTGAAGATCGCGTCATTCTTGAATGAGGCGGGCAATCAGATTTATGTAGACTTAGACCCACCATGTAAATTTGGTATGAAGGTTGGTGATAAAGTTGAGGTTGTTTACCTTTATTTTATTAAGAATACAAGGTCAATTGTTAGAGGTATGGTACTTGGTGCTATATCGAATGTTGTTGTATTACAATCTAAAGGGCATGAAACGGAGGAAGTAGATGCTGTTGGCATTCTTTCTCTTTGTTCTTTTGCAGTAGACCCTGCGGATACGTATTGTAAATATGTGGCAGCAGGTAATCAGCCTTTAGGTAACTGTGTTAAAATGTTGACAGTTCATAATGGTAGTGGTTTTGCTATAACATCAAAGCCAAGTCCAACCCCTGATCAAGATTCTTATGGAGGAGCGTCTGTGTGTCTTTATTGTAGAGCACACATAGCACATCCTGGTGGTGCAGGAAACCTAGACGGACGTTGTCAATTTAAGGGTTCTTTTGTGCAAATACCTACTACGGAGAAAGATCCTGTTGGTTTCTGTCTACGTAATAAAGTTTGTACTGTTTGTCAGTGTTGGGTTGGTTATGGGTGTCAGTGTGATTCACTTAGACAAACACAACCTTCAGTTCAGTCAGCTGTTAGTGCAGCTGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MATSLKQGVSSQNQRSVILVAKDIPDQLCDALFFYTSHKPKDYADALALRQKFDRNLQTGKQFKFETVCGPFLLKGVEKITPGVPAKVLKATSKLADLEDIFGVSPLARKYRELLKTACQWSLTVETLDTRAQTLEEIFDATEILWLQVAAKLQVSAMAMRRLVGEVTVKVMEALGSNLSVLFQIVKEQIARIFQKALAIFEDVSELPQRIFALKMAFAKCAKSITVVVIGHALMIKEFAGTCLASINGAVSKFFEELPNGFMGAKIFSTLAFFKEAAAKIVDNIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKADIPVEPEGWSAILDGHLCYVFRSGDRFYAAPLSGNFALHDVYCCERVVCLVDGVTPEINDGLILAAIYSSFSVTELLAALKKGEPFKFLGHKFVYVKEAAVSFTLAKAATITDVLKLFQSARVKTEDVCSAFTERSFEFWKLAYGKVRNLEEFVKTHFCKAQMSIVILAAVLGEGIWQIVSQIIYKLGGLFSKVVDFCEKHWKGFCGQLKRAKLLVTEQLCVLKGVAQHCFQLLLDAIHSCYKSFKKCALGRIHGDLLFWRGVHKIVQDGDEIWFDAIDSIDVEDLGVVQDKPVDFEVCDDVTLPENQPGHMVQFQHDGNNYMFFRFKKDENIYYTPMSQLGAINVVCKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVEIDLTVEQLLSVIYQKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDCESDDDIEEEDAEECDTGSDDAEECDTASECEEEDEDTKVLALIQDPASTKYPLPLDEDYSVYNGCIVHRDALDVVNLPSGEETFVVNNCFDGAVKPLPQKVIDVLGDWGEAVDAQEKLCEQEPEPFKEPVEQSADVDGGATKQVVAVEQEVIPVVDESQEVVVYTSADLEVVKETSEDVDEFILVSDVSTEEIVSQEGKEPQVELEPIQVVKPQREKKAKKFKVRQTTCKEPKFLEYKTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSYVKGIQCVNNVVGPRHGDKDLEEKLVFAYKNVLVDGVVNYVVPVLSSGIFGVDFKLSIDAMRKAFKGLNIRVLLFSLSQEHIDYFDATCKQKKIYLTEDGVKYRSVIVKPGDSLSQFGQVFAKNKTVFTADDVEDEEVLFIPTTDKAVLEYYGLDAKKYVIYLQTLSQRWKILHKDNFIQLEWRDGNCWINAAIVLLQAANIRFRGFLEEAWAKLVGGDPTDFVAWCYASCNANVGEFSDANWLIANLAEYFEVDYTNALLKKRVSCNCGVKNYELRGIEACIQPVRAPNLLHFKTQYSNCPTCGSNSVDEVVEASLPYLLLLATDGPVSVDCDENVAGNVVFIGSTNSGHCYTQAVGKAFDNLAQDRKFGKRSPYITAMYTRFSLKSENSSSVAKKGEGELVKVDVSSLAISSKVSFDNLTDFEQWYDSNIYESLKVQETPDNMDDYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLIPDNDENSKAPVYYPVLDSISLKAIWIEGSANFVVGHPNYNSRALRIPTFWEFAENFVKIGEKVDGVTMGLWRAENLNKPNLERIFNIIKKMIVGSSVVTTQCGKLISKATTFIADKVGDGVVRNITDRIKGCFGMTRGHFERKISPQFLKTLMFFFFYLVKTSAKSLVASYKIILCKVVFTALLILWFVYTSDPVMFTGIRVLDFLFEGSLCGPYSGYGKESFDVLRYCADDYTCRVCLHGRDSLHLYKHAYSVEQFYKAAVNGISFNWNWLYMLFLILFVKPVAGFVIICYCVKYLVLNSTVLQTGVSFLDWFVQTVFANFNFMGAGFYFWLFYKIYIQVHHILYCKDITCDVCKRVARSNRYEVSVVVSGRKQLVHVYTNSGYNFCKRHNWYCKDCDKYGHQNTFMSPEVAGELSEKLKRHVRPTAFAYHVVDDACVVDGFVNMKYNAATPGKDGLHAAVKCFSVSDFLKKSVFLKDAQKCDQISNDSFIVCNTQSAHALEEAKNAAIYYAQCLCKPILILDQALYERLIVEPVSKSVVDKVCSILSSIVSVDTAALNYKAGTLRDALLSVTEDEEAVDMAIFCHNNDVECTSDGFTNVVPSYGIDTNKLTPRDRGFLINADASIANLKVKNAPPVVWKFSDLVKLSDSCLKYLISATVKSGGRFLITRSGAKQVISCYTQKLLVEKKAGGVVSSTVGAFKSCCKWLLVSYFLLTLCCLGYYQWEMSRDFAQPMYDFNTTMHVEGFKVIDKGVVRDIVPEDTCFSNKFVNFDAFWGKPYDNSRDCPIVTAIIDGVGTIAAGVPGFVGWVMDGVMFIHMTQTERKPWYVPTWFNREIVGYTQDSIITEGSFYTSIALFSARCLYLTASNTPQLYCFNGVNDAPGALPFSSITPHRVYFQPNGVRLIVPQQIMHTPYIVKLVSDSYCRGSVCEYTKPGYCVSMNSQWVLFNGDYTSKPGVFCGSTVRELLFNMVSTFFTGVNPNIYMQLATMFLILVVVVLIFAMVIKFQGVFKAYATIVFTIMLVWVINAFVLCVHSYNSVLAVILLVLYCYASLVTSRNTSIIMHCWLIFTFGLIVPTWLACCYLGFVLYVYTPLFFWCYGTTKNSRKLYEGNEFVGNYDLAAKSTFVIRGSEFVKLTNELGDKFETYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEVVYTPPRYSIGVSRLQAGFKKLVAPSSAVEKCIVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWDDVLNLANNHEFEVTAQNGGTLNVVSRRLKGAVLILQTAVANAGTPKYKFVKANCGDSFTIACSYGGIVVGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLCGEFYGGYVDEEVAQKVPPDNLVTNNVVAWLYAAIISVKESSFSLPKWLDSTTVSVDDYNKWAGDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMVKASQWGCDPILGQYNFEDELTPESVFNQVGGVRLQSSFIRKATSWFWSRCVLACFLFVLCAIVLFTAVPLKYYVHAAAILMVAVLFISFTVKHVMAYMDTFLLPTLVTVIIGVCAEVPFIYNTLISQVVIFLSQWYDPVVFDTMVPWMFLPLVLYTAFKCIQGCYSVNSLNTSLLVLYQFMKLGFVIYTSSNTLTAYTEGNWELFFELVHTTVLANVSSNSLIGLLAFKCVKWMLYYCNATYVNNYVLMAVMVNGMGWLCTCYFGLYWWVNKVFGLTLGKYNFKVSVDQYRYMCLHKIDSPKTVWESLRQIYLYKELGDRVLPISTVQSKLSDVKCSTVVLMQLLTKLNVEANSKMHAYLVDLHNKILASDDVGECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKNLYEKVLADSKNGGVTQQELAAYRKAANIAKSIFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNALFDQASSGVVPLATVPIVCSNKLTLVIPDLETWVKCVEGMHVTYSTVVWNIDTVIDADGIELHPTSTGSGLTYCVSGDNISWPLKVNLTRNVHNRVEAALQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGSSVVAAITSSNPNLKIASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWVGYGCQCDSLRQTQPSVQSAVSAAGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 658 Identity=88.55%
Cov(Q)=9.07% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3949
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
406.355699999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.01712
outside
1 - 1737
TMhelix
1738 - 1760
inside
1761 - 1846
TMhelix
1847 - 1869
outside
1870 - 1883
TMhelix
1884 - 1906
inside
1907 - 2278
TMhelix
2279 - 2297
outside
2298 - 2359
TMhelix
2360 - 2382
inside
2383 - 2555
TMhelix
2556 - 2578
outside
2579 - 2582
TMhelix
2583 - 2605
inside
2606 - 2611
TMhelix
2612 - 2629
outside
2630 - 2648
TMhelix
2649 - 2671
inside
2672 - 3093
TMhelix
3094 - 3116
outside
3117 - 3120
TMhelix
3121 - 3140
inside
3141 - 3151
TMhelix
3152 - 3174
outside
3175 - 3193
TMhelix
3194 - 3216
inside
3217 - 3222
TMhelix
3223 - 3245
outside
3246 - 3254
TMhelix
3255 - 3277
inside
3278 - 3296
TMhelix
3297 - 3319
outside
3320 - 3949
