Strain
Bat_KJ473811
(Region: China; Strain: Severe acute respiratory syndrome-related coronavirus; Date: 2012)
Gene
hypothetical protein ORF3a
Description
Annotated in NCBI,
hypothetical protein ORF3a
Location
GenBank Accession
Full name
Protein 3
Alternative Name
Accessory protein 3
Sequence
CDS
ATGGATTTGTTTATGAGTATTTTCACACTTGGATCAATCACACGTCAACCAAGTAAGATTGAAAATGCTTTTCTTGCAAGTACTGTTCATGCTACTGCAACGATACCGCTACAAGCCTCATTCTCTTTCCGATGGCTTGTTATTGGCGTTGCACTTCTTGCTGTTTTTCAAAGCGCTTCCAAAGTAATTGCGCTTCATAAGAAGTGGCAGCTTGCCTTATACAAAGGCTTTCAATTAGTTTGTAACTTGCTGCTACTCTTTGTGACAATTTATTCACATTTTCTACTTTTAGCTGCTGGCATTGAGGTGCAATTCTTGTACATCTATGCTTTGATTTATATTCTGCAAATTTTAAGCTTTTGCAGATTTATCATGAGATGCTGGCTCTGTTGGAAGTGCAAATCCAAGAATCCATTATTATATGATGCCAACTACTTTGTTTGCTGGCATACATATAATTATGACTACTGTATACCATACAACAGTGTCACAGATACAATCGTCGTTACTACAGGTGACGGCATTTCAACACCAGAACTCAAAGAATACTACCAAATTGGTGGTTATTCTGAGGATTGGCATTCAGGTGTTAAAGACTATGTCGTTGTACATGGCTATTTCGCCGAAGTTCACTACCAGCTTGAGTCTACACAAATTACTACAGACACTGGTATTCAAAACGCTACATTCTTCATCTTTAACAAGCTTGTTAAAGATCCGCCGAATGTGCAAATACACACAATCGACGGCTCTTCAGGAGTTGTAAATCCAGCAATGGACCCAATTTATGATGAGCCGACGACGACTACTAGCGTGCCTTTGTAA
Protein
MDLFMSIFTLGSITRQPSKIENAFLASTVHATATIPLQASFSFRWLVIGVALLAVFQSASKVIALHKKWQLALYKGFQLVCNLLLLFVTIYSHFLLLAAGIEVQFLYIYALIYILQILSFCRFIMRCWLCWKCKSKNPLLYDANYFVCWHTYNYDYCIPYNSVTDTIVVTTGDGISTPELKEYYQIGGYSEDWHSGVKDYVVVHGYFAEVHYQLESTQITTDTGIQNATFFIFNKLVKDPPNVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL
Summary
Function
Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (By similarity).
Subunit
Homotetramer composed of two homodimers linked non covalently. Interacts with M, S and E proteins. Also interacts with the accessory protein 7a (By similarity).
Miscellaneous
Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).
Keywords
Apoptosis
Glycoprotein
Host cell membrane
Host cytoplasm
Host Golgi apparatus
Host membrane
Ion channel
Ion transport
Membrane
Secreted
Transmembrane
Transmembrane helix
Transport
Viral ion channel
Virion
Feature
chain Protein 3
Uniprot
Pubmed
EMBL
Proteomes
Pfam
PF11289
APA3_viroporin
Interpro
IPR024407
SARS_coronavirus_Orf3/3a
ProteinModelPortal
Ontologies
GO
GO:0044385 C:integral to membrane of host cell
GO:0005216 F:ion channel activity
GO:0020002 C:host cell plasma membrane
GO:0051259 P:protein complex oligomerization
GO:0005576 C:extracellular region
GO:0044178 C:host cell Golgi membrane
GO:0019012 C:virion
GO:0039707 P:pore formation by virus in membrane of host cell
GO:0016021 C:integral component of membrane
GO:0005216 F:ion channel activity
GO:0020002 C:host cell plasma membrane
GO:0051259 P:protein complex oligomerization
GO:0005576 C:extracellular region
GO:0044178 C:host cell Golgi membrane
GO:0019012 C:virion
GO:0039707 P:pore formation by virus in membrane of host cell
GO:0016021 C:integral component of membrane
Subcellular Location
From MSLVP
From Uniprot
Virion
The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host Golgi apparatus membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cell membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Secreted The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cytoplasm The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host Golgi apparatus membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cell membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Secreted The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cytoplasm The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Topology
Length:
274
Number of predicted TMHs:
3
Exp number of AAs in TMHs:
65.2254500000003
Exp number, first 60 AAs:
18.84472
Total prob of N-in:
0.03376
POSSIBLE N-term signal
sequence
outside
1 - 43
TMhelix
44 - 66
inside
67 - 78
TMhelix
79 - 101
outside
102 - 104
TMhelix
105 - 124
inside
125 - 274
