Strain
Avian_KM213963
(Region: China; Strain: Infectious bronchitis virus isolate CK/CH/XDC- 2/2013, complete genome.; Date: 2013)
Gene
polyprotein 1a
Description
Annotated in NCBI,
polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTTCAAGCCTAAAACAGGGAGTATCTTCCCAAAACCAGAGGAGTGTCGTTCTTGTTGCCAAAGACATTCCCGATCAACTCCGTGACGCTTTGTTTTTCTATACGTCACACAACCCAAAAGATTACGCTGATGCTTTCGCTTTTAAGCGGAAGTTTGATCGTAATCTGGGCGCTGGGAAGCAGTTTAAATTTGAAACTGTGTGTGGTCCATTCTTTCTTAAAGGAGTGGATAAAGTAACACCAGGGGTCCCAGCTAAAGTTTTGAAAGCCACTTCAAAATTGGCTGATTTAGAAGACATCTTTGGTGTCTCACCTCTTGCACGGAAATACCGTGAACTTCTTAAAACAGCATGCCAGTGGTCTCTTACTGTAGAAGCGCTGGATGCTCGTGCCCAAACTCTTGAAGAAATTTTTGAACCAACTGAAGTACTTTGGCTCCAGGTGGCTTCAAAACTTCAGGTTTCAGCTATGGCAATGCGCAGACTTGTTGGTGAAGTAACTGCAAAAGTCATGGATGCTTTGGGCTCAAAATTGAGTTCTCTTTTTCAAATTGTGAAACAGCAAATAGCCAGAATTTTTCAGAAAGCTCTGGCTATTTTTGAAGATGTAAAAGACTTACCACAACGCATAGCTGCACTGCAGATGGCTTTTGCAAAGTGCGCTAAAACAATTACTGTTGTTGTTGTGGAAAAAGCTATTGTTGTTCGCGAATTTTCTGGAACTTGTCTTGCAAGTATTAATGGTGCCATTGCTAAGTTCTTTGCGGAACTACCCAATGGTTTTATGGGAGCTAAAGTTTTTAGCAAGCTGGCTTTCTTTAAAGAGGCAGCTGCTAAAATTGTAGAGAATGTTCCAGGAGCACCGCAAGGTACTAAAGGTTTTGAAATTGTTGGCAATGCTAAAGGCACACAGGTTGTAGTTCGTGGCATGCGCAATGACTTAACACTGCTTGACCAAAAAGCTGAGATTCCTGTTGAACCAGAGGGCTGGTCCGCAATCTTGAATGGACATCTTTGCTATGTCTTTAAGAGTGGTGACAGATTTTATGCTGCGCCTTTGTCAGGAAATTTTGCACTTCATGATGTTCATTGCTGTGAACGTGTTGTATGTCTAGTTGATGGTGTTACACCAGAGATTAATGACGGCCTTATTCTCGCAGCAATCTACTCATCGTTTAGTGTGGTTGAACTTGTGGCAGCACTTAAGAAAGGTGAACCATTTAAGTTTTTAGGCCATAAATTTGTGTATGTAAAGGATGCGGCGGTTACCTTCACACTGGCAAAAGCAGCAACTATTTCCGATGTCTTGAAGCTGTTTCAATCAGCAAGAGTCAAGACTGAGGATGTGTGGTCTGCACTTACTGAAAAGTCTTTTGAATTCTGGAAATTCGCATATGGAAAAGTGCGAAATCTTGAGGAATTTGTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTGTGGTTTTAGCAGTTGTGCTTGGTGAAGGCATTTGGTATCTTGTTTCGCAAGTCATCTATAAGTTGGGTGGTTTGTTTTCTAAAGTTGTTGATTTTTGTGCAAAGTACTGGAAAGGTTTCTGTGAACAATTGAAGAAGGCTAAGCTTCTTGTTACAGAACACCTATGTGTCTTGAAAGGTGTTGCACAGCATTGTTTCCAACTTCTGCTGGATGCAATACACTCTATGTACAAGAGTTTTCAAAAGTGCGCACTTGGTAGAATCCATGGGGATTTACTCTTCTGGAAAGGAGGCGTACATAAAATAGTTCAAGAAGGCGATGAAGTTTGGTTCGACGCTATTGATAGTATTGATGTTGAAGACCTGGGTGTTACCCGAGAAAAACCTGTAGACTTTGAGGTTTGTGATGGTGTAACACTTCCAGAAAATCAACCCGGTCATATGGTTCAGTTTGAAGATGATGGAAAGAACTATATGTTCTTCCGCTTCAAAAAGAATGACAATGTTTACTATACACCAATGTCACAACTTGGTGCAATTAATGTAGTTTGCAAAGCAGGTGGCAAGACTGTCACGTTTGGTGAAACTACTGTGAAAGAAATACCACCACCCGATGTAGTGCCTATTAAGGTTAGCATCGAGTGTTGTGGCGAACCATGGAATACAATCTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAAACAGACCTCACAGTAGAACAATTGCTCTCTGTGATCTACGAGAAAATGTGTGACGACCTCAAACTGTTTTCTGAGGCCCCAGAACCTCCACCATTTGAGAACGCTGTGATCTACGAGAAAATGTGTGACGGTAAAGATTTGGATTGCATAAAATCATGCTACCTTATTTACCGTGATTATGAGAGTGATGGTGAAATTGAAGAGGAAGATGCTGAGGAATGTGACACAGCTTCAGATAATGCTGAGGAATGTGACACAGCTTCGGAGTGTGATGAAGAGGATGAAGGTACCAAGGTGTTGGCTCTTATACAAGACCCAGCAAGTCAAAAATACCCTCTGCCTCTTGATGATGATTACAGCGTTTATAATGGATGTATAGTCCACAGAGATGCGCTTGATGTTGTGAATCTACCATCTGGTGAAGAAACTTTTGTTATTAACAATTGTTTTGAGGGAGCTGTAAAAGAACTCCCAAAGTCAGTTGTAGATGTGCTTGGTGACTGGGGTGAAGCCGTTGATGCACAGGAGAAATCCTTGAATGACGTAGCTGATTCCAGTAATGTGTTATTGCCGGGTGAAAATCCGGTAATTGACATATGTGGTGAACAGGAAGTTATTCCTGTTGTTGCAGATGAGCTGGACGTTATAGTTTTCACGCCAGCGGAATTAGATGTTGTAAAGGAAGCTCCTGGAGAGGTTGACGAGTTCGTCCTCATCCCAAGTGTTTCTAGTGGTGAAGAAGTGGTTTCTGTTGTGAGTGAAAATGTCACGCCACAGCAGGAAGAACCTAGAGAAGAAGTTGTTCCTGACATTGTTTTGCAGCAGGAAGAGCTTAAAATTGAAGAGCCTCCTCAAGTTGAAAAATCACAACGTGAGAAGAAAGCTAAGAAGTTTAAAGTCAAAACAACTACACAACAAGCTCCTAAGTGTTTGGAGTATAAAACTTGTGTGGGTGACTTGACTGTTGTGATTGCCAAAGCATTGGACGAGTTTAAAGAGTTCTGCGTTGTAAACGCTGCAAATGAGCATATGTCTCATGGCGGTGGCGTTGCAAAAGCAATTGCAGACTTTTGTGGTCCAGATTTTGTGGATTATTGTGAAGACTATGTTAAGAAACATGGTCCTCAGAAGAGACTTATCACACCTTCTTATGTTAAAGGCATTCAATGTGTGAACAATATTGTAGGCCCCCGCCATGGAGACAGTGATTTGTTTCAAAAGCTTGTCACTGCGTACAAGAGTGTTATTGTAAATGGCGTGGTCAATTACGTTGTGCCAGTTCTTTCATCTGGAATTTTTGGAGTTGATTTTAAGTTGTCAATAGATGCTATGCGTGCAGCCTTTGAGGATTGCAACATACGCGTTCTTTTGTTTTCTTTGAATCAAGAGCACATCGATTATTTCGATGCAACTTGCAAACAGAAGACAATTTATCTTACAGAAGATGGTGTTAAATACCGTTCTGTAATTGTTAAACCTGGAGATTCGTTGAGTCAATTTGGACAGGTTTTTACGCGAAACAAAACAGTCTTTACTGCGGATGATGTTGAGGATAAAGAAATTTTATTCATTCCAACTACAGACAAGGCTGTTTTAGATTACTATGGTTTGGATGCGAAAAAGTATGTGATATATTTACATACGCTTTCACAAAAATGGAAAATCCTAGTTAAGGACAACTTTATCCAACTTGAGTGGCGTGATGGAAATTGTTGGATTAATTCAGCAATAGTACTCCTTCAGGCTGCTAAGATTAAATTTAAAGGTTTTCTTGCAGAAGCATGGGCTAAACTGTTGGGTGGAGATCCTACAGATTTTGTAGCTTGGTGCTATGCAAGTTGCAATGCTAATGTTGGTGATTTTTCAGACGTCAATTGGCTTTTGGCTAATTTGGCAGAGTATTTTGAAGTAGATTACACGAATGCACTTCTTAAGAAGCGTGTTTCTTGTAATTGTGGCATTAAGAGTTATGAGTTTAGAGGCCTTGAAGCTTGTATTCAGCCAGTAAAGGCACCTAATCTCTTACATTTTAAGACGCAGTATTCAAACTGTCCTACTTGTGATTCAAATAGTGTAGATGAAGTGATAGAAGCCTCACTGCCTTATTTGCTATTACTTGCAACAGATGGTCCTGCAACGGTTGATTGTGATGAAAGTGTTGTAGGAAATGTAGTCTTCATTGGATCCACTAATAGTGGTCATTGTTATACTCAAACTGCTGGTAAAGCTTTTGACAATCTTGCTAATGATAGGAAATTTTCAAAGAGGTCGCCTTACATTACAGCAATGTATACGCGCTTCTCTTTTAAAAATGAAAATTCTGTGCCTGTTGCTAAGAAGAGCAAAGGTAAAAGTCAAGTAGTAAAAGAAGATGTTTCTAGCCTTGCTATTAGTTCAAAAACCAGTTTTGATGATCTCACTGACTTTGAGCAATGGTATGATAGTAACATCTACGATAGTCTTAAAGTTCAGGAAACACCTGACAATTTTGATGAATATGTGTCATTTACAACAAAGGAGGATTCCAAGTTGCCATTGACACTTAAAGTCCGTGGTATTAAGTCAGTTGTGGATTTTAGGTCAAAGGATGGTTTTATCTATAAATTAACACCTGATACTGATGAAAATTCAAAGGCACCCGTTTACTACCCCGTCTTAGACTCTATTAGTCTTAAGGCAATTTGGGTAGAAGGCAGTGCTAACTTTATTGTTGGACATCCAAATTATAATAGTAGAGCTCTGCGAATTCCCACCTTCTGGGAGTGTGCAGAGAGTTTTGTTAAAATTGGTGAAAAAGTTGATGGTGTTACTATGGGCTTATGGCGCGTAGAATACCTTAACAAACCCAATTTGGAGAAGCTTTTCAACATTGTTAAGAAAACAATTGTTGGAACTAATATTGTTACTACTCAGTGTGGTAAATTAATTGCTAAGGCTACATCATTTATAGCTGATAAAGTAGGAGATGGTGTAGTTCGCAATGTTACTGATAGAATTAAAGGGTGTTTTGGGTTTACTAGGGAGCATTTTGAAAGAAGAATGCCTCCACAGTTCTTAAAAACTCTCTTCTTCTTTTTCTTTTGTCTCTTGAAGGCTAGTGCTAAGAGTTTATTGGCTAGCTACATGATTGTGTTATGTAAAGTGTTGTTTACTGCTTTACTTATATTATGGTTTGTCTACACAAGTAATCCAGTTATGTTCACCGGAATACGTGTGTTAGATTTCCTATTTGAAGGTTCATTTTGTAGTCCTTATGTAGATTACGGTAAAGAATCTTTTGATATTTTACGCTATTGTGGAAGTGACTTCACTTGTCGTGTTTGTTTACATGGTAGGGATTCGCTTCATCTGTATAAACATGCCTATAGCGTAGAACAGTTTTACAAAGATGCAGTTAATGGTATCAGTTTTACTTGGAATTGGCTTTATATGCTGTTCCTAATACTGTTTGTTAAGCCAGTGGCAGGTTTTGTTATTATTTGCTACTGTATAAGATATTTAGTTTTAAGCACGACTGTATTGCAAACTGGTGTAGGATTTTTAGACTGGTTTATTCAAACAGTGTTTGCTAACTTTAATTTTATGGGTGCAGGATTTTATTTCTGGCTCTTTTACAAGATATATATACAGGTTCATCATATAATGTACTGTAAGGATATAACATGTGAAGTGTGTAAGAGAGTTGCACGCAGTAATAGACATGAGGTTAGTGTAGTTGTTAATGGTCGTAAACAGCTCGTTCACGTCTACACTAATTCAGGCTATAATTTCTGTAAGAAACATAATTGGTATTGTAGGAATTGTGACAAGTATGGTCACCAAAATACATTTATGTCTCCTGAAGTTGCTGGCGAGCTTTCTGAAAAGCTTAAGCGTCATGTTAAACCTACAGCATCCGCATATCATGTAGTGGATGATGCGTGCGTAGTTGATGAATTTATTAATCTGAAGTACAATGCAGCCACCCCAGGTAAAGAAGGTGTGCATTCTGCTGTTAAATGTTTTAGTGTATCTGATTTCTTAAAGAAAGCAGTCTTTCTTAAGGATGCACAAAAATGTGAACAAATAGCTAATGATAGTTTTATAGTTTGTAATACACACAGTGCGCATGCTTTAGAGGAAGCTAAAAATGCTGCCATCTATTATGCGCAATGTCTTTGTAAACCAATTCTTATACTTGATCAGGCTCTTTATGAACAATTGGTTGTAGAACCTGTGTCCAAGAGTGTTGTAGACAAAGTGTGTAATATTTTGTCTAACATAATATCTGTAGATTCTGCAGCTTTGAATTATAAGGCAGGCACACTTCGTGATGCCCTCCTTTCTACTACTAAGGATGAAGAAGCTGTTGATATGGCTATCTTCTGCCATAATAATGATGTTGAATATACTAATGATGGTTTTACTAATGTTGTGCCATCATATGGTATAGACACTGATAAGTTAACATCTCGAGATAGAGGCTTTTTGATAAATGCAGATGCTGCTATTGCTAATTTGAAAGTGAAAAATTCACCGCCAGTAGTGTGGAAGTATTCTGATCTTGTCAAACTGTCTGACAGTTGTCTTAAGTATTTAATATCAGCAACTGTCAAAGCAGGAGGTAGGTTTTTCATAACGAGGTCTGGTGCTAAACAAGTTATTGCTTGTTACACGCAGAAGTTGTTAGTGGAAAAGAAGGCTGGTGGTGTCGTTAGTGGCACTGTTAGTTTGTTTAAGAGTTGTTTTAAGTGGCTCTTAGTATTTTATTTACTTTTTACAGCGTGTTGTTTGGGCTATTATCAATGGGAAATGAGTAGAGAATTTGCTCACCCCATGTATGATGTTAACTCCACAATGCATGTAGAAGGATTTAAGGTTATTGACAAGGGTGTACTCAGGGATATTATGCCAGAAGACACTTGCTTCTCTAATAAGTATGTAAATTTTGACCCATTTTGGGGAAAACCTTATGTAAATAATAGAGACTGTCCTATTGTCACCGCTATTATAGATGGTGCTGGAACAGTGGCTGCTGGTGTTCCTGGTTTTGTACAGTGGGTCATGGATGGTGTAATGTTTATCCATATGACACAAACTGAGCGAAAACCTTGGTACATTCCTACATGGTTTAATAGAGAAATTGTTGGTTATACTCATGATTCTATTATTACAGAAGGTGAGTTTTATACTTCCTTAGCATTGTTTGCTTCTAGGTGTTTGTATTTAACATCTAGTAACACACCACAGCTTTATTGCTTTAATGGTGATAGCGATGCTCCCGGTGCATTGTCGTTCGCAAATATACTCCCTCATAGGGTATATTTTCAACCTAATGGAGTTAGGCTTATTGTGCCTCAGCAAATTATGCATACACCATACATAGTTAGACTTGTTTCAGACAGCTATTGTAGAGGTAGTGTTTGTGAGTATACTAGACCAGGTTATTGTGTATCACTGAATTCCCAATGGGTTTTGTTTAATGACGAATACATAAGTAAACCAGGTGTGTTTTGTGGCTCTACTGTTAGAGAGCTTTTGTTTAGTATGGTTAGTACATTCTTCACGGGTGTTAATCCAAATATCTATATGCAACTGGCAACTATGTTTTTAATACTTGTTGCTGTTGTGTTAATATTTGCAATGGTTATAAAGTTTCAAGGTGTTTTCAAAACTTATGCAACTACTGTATTTACTATAATGCTTGTCTGGGTTATTAATGCATTTGTTTTATGTGTGCATAGTTATAATAGTGTTTTAGCTATTATACTACTTGTGCTTTATTGTTATGCATCACTTGTGACGAGCCGTAATACTGCTATAATAATGCACTGTTGGCTTGTTTTCACATTTGGTTTAATAGTACCTAGTTGGGTAGCTTGTGTCTATTTAGGTTTTGTGCTATATATGTATACACCGCTGGTTTTGTGGTGTTATGGTACAACTAAAAATAGTCGCAAGCTTTATGAAGGCAATGAGTTTGTTGGCACTTATGATCTTGCTGCGAAGAGTACTTTTGTAATTCGTGGGCCTGAATTTGTTAAACTTACGAATGAGCTAGGCGACAAATTTGAAACTTATCTCTCAGCGTATGCTAGACTTAAATACTACTCAGGCACTGGCAGTAAGCAGAATTACTTGCAAGCATGTCGTGCATGCTTAGCTTATGCACTAGATCAATTTAGAAAAGGCGGTGTAGAAACTGGTTATACTCCACCACGGTATTCAATTGGTGTTAGTAGATTACAGGCTGGTTTCAAGAAACTAGTTGCTCCTAGTAGTGCTGTTGAGAAGTGCATTGTTAGTGTATCCTATAGGGGTAATAATCTTAATGGGTTGTGGTTGGGTGATTCTATTTACTGTCCACGTCATGTGTTAGGTAAGTTTAGTGGTGATCAATGGGTTGATATACTAAACCTTGCTAATAATCATGAGTTTGAAGTTGTAACTGGAAAAATGGGTGTTACTTTGAATGTCATCAGCAGGCGTTTGAGAGGTGCGGTTTTAATTTTACAAACTGCAATTGCCAATGCTGAAACTCCAAAGTATAAATTTTTGAAAGCAAATTGTGGTGATAGTTTTTCATTGGCTTGCTCTTATGGTGGTACAGTTATAGGACTTTACCCCGTCACTATGCGTTCTAATGGAACTATTAGAGCATCTTTTCTTGCTGGAGCATGTGGTTCAGTAGGTTTTAATATAGAAAAGGGTGTAGTAAATTTTTACTATATGCACCATCTAGAGTTACCTAATGCATTACACACTGGAACTGACTTAACGGGTGAGTTCTATGGTGGCTATATAGATGAGGAGGTTGCTCAGAAAGTTCAACCTGACAAATTAGTTACTAATAATATTTTGGCATGGCTTTATGCAGCAATTATTAGTGTTAAGGAGAGTAGTTTTTCAACACCTAAATGGCTTGAAAGTACTACTGTCAGTATTGATGATTATAATAAGTGGGCAGCGGATAATGGTTTCACATCATTTGTGAGCTGCACTGCTATTACTAAATTAAGTGCTATAACAGGAGTTGATGTCTGTAAGCTCCTTCGCACTATTATGGTGAAAAGTACACAATGGGGTAGTGACCCCATTTTGGGACAGTATAATTTTGAGGATGAACTGACACCAGAATCTGTTTTTAATCAGGTGGGTGGTGTTAGACTACAATCATCTGTTGTAAAGAAAGCTGCGTCTTGGTTTTGGAGTAGATGTGTTCTAGCTTGCTTCCTCTTTGTCTTGTGTTCTATTGTGCTGTTTACAGCCATACCATATAGATATTATTTACATGGCGCTGTTGTTTTACTTGCAACTGTGCTTTTTATTTCTTTTACTGTGAAACATGTTATGGCATATATGGATACTTTCCTTTTGCCGACATTGATTACAGTTATTATAGGAGTTTGTGCTGAAGTACCTTTCATCTACAATACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTGGTCTTTGACACTGTAGTACCGTGGATGTTCTTGCCTTTAATCTTATATACATCTTTTAAATGTATACAAGGTTGTTATAGTGTAAATTCCTTTAATACTTCTCTGTTATTGTTGTATCAGTTTATGAAGTTGGGTTTTGTTATATCCACTTCTTCTAATACTCTTGCAGCATATTCAGAGGGTAATTGGGAGTTGTTCTTTGAGTTAGTTCATACTACTGTGTTGGTTAATGTTAGTAGCAATTCTTTAATTGGCTTGTTTGTTCTTAAATTGGCTAAGTGGATGCTGTATTATTGTAATGCATCATACCTTAATAATTATGTTCTAATGGCTGTCATTGTTAATGGCATAGGTTGGATGTTTACCTGTTACTTTGGGTTCTATTGGTGGATTAATAAGGTTTTTGGTTTAACCTTAGGCAAATATAATTTTAAAGTTTCAGTAGACCAATATAGGTATATGTGTCTTCATAAGATTGACCCACCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGGATAGGCGGTGAGCGCGTGCTTCCTATAGCCACGGTTCAATCTAAATTAAGTGATGTAAAATGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTAGAGTTACACAATAAAATTCTTACATCTGATGATGTTAATGAGTGCATGGATAATTTATTGGGTATGCTTGTAACATTATTTTGTGTAGATAGTACTATAGATTTAAGTGAGTATTGTGATGATATACTTAAGAGATCAACTGTTTTACAATCAGTTACTCAGGAATTTTCACATATTCCTTCTTATGCAGAGTATGAAAGAGCTAAAGATCTTTATGAAAAGGTCTTAGCTGATTCTAAAAATGGTGGTGTTACGCAGCAAGAACTTGCTGCATATCGTAAAGCTGCCAATATTGCTAAGTCGATTTTTGATAGAGATTTGGCTGTTCAGAAGAAGTTGGATAGCATGGCTGAACGTGCTATGACAACTATGTATAAGGAAGCGCGTGTTACTGATAGGAGGGCGAAACTTGTTTCGTCATTACATGCGCTATTATTTTCTATGCTTAAGAAGATAGATTCTGAAAAGCTTAATGTACTATTTGACCAAGCAAGCAGTGGTGTTGTGCCACTGGCTACTGTTCCAATAGTTTGTAGTAATAAGCTTACTCTTGTAGTGCCAGACCCAGAAACATGGGTCAAGTGCGTAGAGGGCATGCATGTTACATATTCAACAGTTGTTTGGAATATTGATAATGTCGTTGATGCGGATGGCACTGAATTGCAACCAACTTCTACAGGTAATGGTTTAGCCTACTGTATAAGTGGTGATAATATGGCATGGCCCCTTAAGGTCAATTTGACTAGGAATGCGCATAATAGAGTTGATGCAGTATTGCAGAACAACGAGCTTATGCCTCATGGTGTTAAAACAAAAGCTTGCGTGGCAGGTGTGGACCAAGCACACTGCAGCGTAGAGTCTAAATGTTACTATACTAATATTAGTGGTAATTCAGTTGTTGCTGCTATTACTTCTTCTAATCCAAATTTGAAAGTTGCCTCATTCTTGAATGAAGCAGGCAATCAAATCTATGTTGACTTAGACCCACCATGTAAATTTGGCATGAAGGTGGGTGATAAAGTAGAAGTTGTTTACTTGTATTTTATAAAGAATACAAGGTCTATTATTAGAGGTATGGTACTTGGTGCTATATCTAATGTTGTGGTGTTACAGTCTAAAGGACATGAGACAGAGGAAGTAGATGCTGTTGGCATACTATCACTTTGTTCTTTTTCAGTAGATCCAGTTGACACGTATTGTAAGTATGTAGCAGCAGGTAATCAACCTTTAGGCAACTGTGTTAAGATGTTGACAGTTCATAATGGTAGTGGCTTTGCTATAACATCAAAACCAAGTCCAACTCCTGATCAGGATTCTTATGGAGGAGCTTCTGTGTGTCTCTATTGTAGAGCACATATAGCTCACCCCGGAAGTGCAGGAAATTTAGATGGACGTTGTCAATTTAAAGGTTCTTTTGTTCAAATACCTACTACGGAGAAAGACCCTGTTGGGTTTTGTTTACGTAATAAGGTTTGCACTGTTTGTCAGTGTTGGATTGGTTATGGCTGTCAATGTGATGCACTTAGACAACCCAAACCTTCTGTTCAGTCAGCTGATGGGGCAGCAGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSSQNQRSVVLVAKDIPDQLRDALFFYTSHNPKDYADAFAFKRKFDRNLGAGKQFKFETVCGPFFLKGVDKVTPGVPAKVLKATSKLADLEDIFGVSPLARKYRELLKTACQWSLTVEALDARAQTLEEIFEPTEVLWLQVASKLQVSAMAMRRLVGEVTAKVMDALGSKLSSLFQIVKQQIARIFQKALAIFEDVKDLPQRIAALQMAFAKCAKTITVVVVEKAIVVREFSGTCLASINGAIAKFFAELPNGFMGAKVFSKLAFFKEAAAKIVENVPGAPQGTKGFEIVGNAKGTQVVVRGMRNDLTLLDQKAEIPVEPEGWSAILNGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLVDGVTPEINDGLILAAIYSSFSVVELVAALKKGEPFKFLGHKFVYVKDAAVTFTLAKAATISDVLKLFQSARVKTEDVWSALTEKSFEFWKFAYGKVRNLEEFVKTHFCKAQMSIVVLAVVLGEGIWYLVSQVIYKLGGLFSKVVDFCAKYWKGFCEQLKKAKLLVTEHLCVLKGVAQHCFQLLLDAIHSMYKSFQKCALGRIHGDLLFWKGGVHKIVQEGDEVWFDAIDSIDVEDLGVTREKPVDFEVCDGVTLPENQPGHMVQFEDDGKNYMFFRFKKNDNVYYTPMSQLGAINVVCKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYEKMCDDLKLFSEAPEPPPFENAVIYEKMCDGKDLDCIKSCYLIYRDYESDGEIEEEDAEECDTASDNAEECDTASECDEEDEGTKVLALIQDPASQKYPLPLDDDYSVYNGCIVHRDALDVVNLPSGEETFVINNCFEGAVKELPKSVVDVLGDWGEAVDAQEKSLNDVADSSNVLLPGENPVIDICGEQEVIPVVADELDVIVFTPAELDVVKEAPGEVDEFVLIPSVSSGEEVVSVVSENVTPQQEEPREEVVPDIVLQQEELKIEEPPQVEKSQREKKAKKFKVKTTTQQAPKCLEYKTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVDYCEDYVKKHGPQKRLITPSYVKGIQCVNNIVGPRHGDSDLFQKLVTAYKSVIVNGVVNYVVPVLSSGIFGVDFKLSIDAMRAAFEDCNIRVLLFSLNQEHIDYFDATCKQKTIYLTEDGVKYRSVIVKPGDSLSQFGQVFTRNKTVFTADDVEDKEILFIPTTDKAVLDYYGLDAKKYVIYLHTLSQKWKILVKDNFIQLEWRDGNCWINSAIVLLQAAKIKFKGFLAEAWAKLLGGDPTDFVAWCYASCNANVGDFSDVNWLLANLAEYFEVDYTNALLKKRVSCNCGIKSYEFRGLEACIQPVKAPNLLHFKTQYSNCPTCDSNSVDEVIEASLPYLLLLATDGPATVDCDESVVGNVVFIGSTNSGHCYTQTAGKAFDNLANDRKFSKRSPYITAMYTRFSFKNENSVPVAKKSKGKSQVVKEDVSSLAISSKTSFDDLTDFEQWYDSNIYDSLKVQETPDNFDEYVSFTTKEDSKLPLTLKVRGIKSVVDFRSKDGFIYKLTPDTDENSKAPVYYPVLDSISLKAIWVEGSANFIVGHPNYNSRALRIPTFWECAESFVKIGEKVDGVTMGLWRVEYLNKPNLEKLFNIVKKTIVGTNIVTTQCGKLIAKATSFIADKVGDGVVRNVTDRIKGCFGFTREHFERRMPPQFLKTLFFFFFCLLKASAKSLLASYMIVLCKVLFTALLILWFVYTSNPVMFTGIRVLDFLFEGSFCSPYVDYGKESFDILRYCGSDFTCRVCLHGRDSLHLYKHAYSVEQFYKDAVNGISFTWNWLYMLFLILFVKPVAGFVIICYCIRYLVLSTTVLQTGVGFLDWFIQTVFANFNFMGAGFYFWLFYKIYIQVHHIMYCKDITCEVCKRVARSNRHEVSVVVNGRKQLVHVYTNSGYNFCKKHNWYCRNCDKYGHQNTFMSPEVAGELSEKLKRHVKPTASAYHVVDDACVVDEFINLKYNAATPGKEGVHSAVKCFSVSDFLKKAVFLKDAQKCEQIANDSFIVCNTHSAHALEEAKNAAIYYAQCLCKPILILDQALYEQLVVEPVSKSVVDKVCNILSNIISVDSAALNYKAGTLRDALLSTTKDEEAVDMAIFCHNNDVEYTNDGFTNVVPSYGIDTDKLTSRDRGFLINADAAIANLKVKNSPPVVWKYSDLVKLSDSCLKYLISATVKAGGRFFITRSGAKQVIACYTQKLLVEKKAGGVVSGTVSLFKSCFKWLLVFYLLFTACCLGYYQWEMSREFAHPMYDVNSTMHVEGFKVIDKGVLRDIMPEDTCFSNKYVNFDPFWGKPYVNNRDCPIVTAIIDGAGTVAAGVPGFVQWVMDGVMFIHMTQTERKPWYIPTWFNREIVGYTHDSIITEGEFYTSLALFASRCLYLTSSNTPQLYCFNGDSDAPGALSFANILPHRVYFQPNGVRLIVPQQIMHTPYIVRLVSDSYCRGSVCEYTRPGYCVSLNSQWVLFNDEYISKPGVFCGSTVRELLFSMVSTFFTGVNPNIYMQLATMFLILVAVVLIFAMVIKFQGVFKTYATTVFTIMLVWVINAFVLCVHSYNSVLAIILLVLYCYASLVTSRNTAIIMHCWLVFTFGLIVPSWVACVYLGFVLYMYTPLVLWCYGTTKNSRKLYEGNEFVGTYDLAAKSTFVIRGPEFVKLTNELGDKFETYLSAYARLKYYSGTGSKQNYLQACRACLAYALDQFRKGGVETGYTPPRYSIGVSRLQAGFKKLVAPSSAVEKCIVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWVDILNLANNHEFEVVTGKMGVTLNVISRRLRGAVLILQTAIANAETPKYKFLKANCGDSFSLACSYGGTVIGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLTGEFYGGYIDEEVAQKVQPDKLVTNNILAWLYAAIISVKESSFSTPKWLESTTVSIDDYNKWAADNGFTSFVSCTAITKLSAITGVDVCKLLRTIMVKSTQWGSDPILGQYNFEDELTPESVFNQVGGVRLQSSVVKKAASWFWSRCVLACFLFVLCSIVLFTAIPYRYYLHGAVVLLATVLFISFTVKHVMAYMDTFLLPTLITVIIGVCAEVPFIYNTLISQIVIFFSQWYDPVVFDTVVPWMFLPLILYTSFKCIQGCYSVNSFNTSLLLLYQFMKLGFVISTSSNTLAAYSEGNWELFFELVHTTVLVNVSSNSLIGLFVLKLAKWMLYYCNASYLNNYVLMAVIVNGIGWMFTCYFGFYWWINKVFGLTLGKYNFKVSVDQYRYMCLHKIDPPKTVWEVFSTNILIQGIGGERVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILTSDDVNECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKDLYEKVLADSKNGGVTQQELAAYRKAANIAKSIFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWVKCVEGMHVTYSTVVWNIDNVVDADGTELQPTSTGNGLAYCISGDNMAWPLKVNLTRNAHNRVDAVLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIIRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFSVDPVDTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDALRQPKPSVQSADGAAGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 617 Identity=85.47%
Cov(Q)=9.01% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3972
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
387.76613
Exp number, first 60 AAs:
0
Total prob of N-in:
0.04454
outside
1 - 1759
TMhelix
1760 - 1782
inside
1783 - 1867
TMhelix
1868 - 1890
outside
1891 - 1904
TMhelix
1905 - 1927
inside
1928 - 2299
TMhelix
2300 - 2318
outside
2319 - 2380
TMhelix
2381 - 2403
inside
2404 - 2576
TMhelix
2577 - 2599
outside
2600 - 2608
TMhelix
2609 - 2628
inside
2629 - 2634
TMhelix
2635 - 2657
outside
2658 - 2671
TMhelix
2672 - 2694
inside
2695 - 3115
TMhelix
3116 - 3138
outside
3139 - 3142
TMhelix
3143 - 3160
inside
3161 - 3172
TMhelix
3173 - 3195
outside
3196 - 3209
TMhelix
3210 - 3232
inside
3233 - 3244
TMhelix
3245 - 3267
outside
3268 - 3276
TMhelix
3277 - 3299
inside
3300 - 3318
TMhelix
3319 - 3341
outside
3342 - 3972
