Strain
Avian_KM454473
(Region: China; Strain: Duck coronavirus isolate DK/GD/27/2014, complete genome.; Date: 2014)
Gene
ORF1a
Description
Annotated in NCBI,
ORF1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCCCCAGGCCTTAGCAAGGGAGTATCTCCCAATAAAAAGAATGTCATTTTTGTAGTGAAAGACATTCAGCCAGCATTGTGTGATGCTTTGTTTTTCTATACATCACACGTTCCACGCGATTTCGCTGATGCGCATACCGTTCGTGAACGGTTTGATCGCAATTTGCAAAGCGGTCGCGTTTTTAAGTTTGAGACTGTGTGTGGCCCTGTGTATTTGCAGGGTGTACCCTCAGTCCCACCAGGTGTCAAGGCTTTGGATGCTGAATCTAAGCTTGTTGATTTGGAAAATATTTTTGGTGTTTCACCTCTGTCTCGGCGTTATAGAGACCTTTTGAAGACGGCACCACAGTGGTCGTTGAATGTTGAATCTCTTGATGCCCAGGCGGAAAAATTGGACACTCTTTTTAGTTCTTCAGAGATTTTGTGGCTTCAGGTTGCCGCTAAGGTCCAAGTGTCCGCTATGGCACTGCGCAGACTTGTTGGTGAGGTTACTGCAAAAGTCATGGATTCACTTGGCTCAAATTTGAGCGCTCTGTTCCAAGTTGTTAAGGAAACAATGGCCAGAATCTTTCAAAAGGCTTTGGCCATTTTTGAGAGTGTGAGTGAATTGCCACAACGTATTGCTGCACTTAAGATGGCTTTTGCCAAGTGTGCAAGGTCAATTACTGTTGTGGTTGTTGGACATGCCCTCACTATTAGAGAGTTTGCTGGAACTTGTCTTGCAAGCATTAATGGTGCAGTTGCAAAATTCTTTGAAGAACTTCCAACTGGCTTTATGGGCGCTAAGGTCTTTTCAACCCTTGCCTTCTTTAAAGAAGCAGCAGTAAAGATTGTAGAAAATATACCAAATGCGCCAAGAGGTACTAAAGGTTTTGAAGTCGTTGGCAATGCCAAAGGTACACAAGTTGTTGTCCGTGGCATGCGAAACGACTTAACTCTGCTTGACCAAAAAGCTGAAGTTCCTGTGGAACCAGAAAGTTGGTCTGCAATTTTGGAGGGACATCTTTGCTATGTCTTTAAGAGTGGTGATCGCTTTTATGCGGCACCTCTTTCAGGAAATTTTGCATTGCATGATGTGCATTGTTGTGAGCGTGTGGTCCTCCTTAGTGATGGCTACACTCCTGAAATTAATGATGGTTTGCTTTTGGCAGCGCTTTATACGTCATCTACTGTGGCTGATGTGTTGGCTGCGCTGAAGAAGGGTGAGCCATTTAAGTTTTTGGGACACTCGTTTGTCTATGTCAAGGACGCAACTGTGTCTTTCACTTTGGCAAAGGGTGCATCTATAGCAGATGTTCTTAAACTTTTTGTTAAAGAAGCTGCCGATGTTTGGTCTGTTTTTAACGAAAAGTCTCATGAATTTTGGACTAGAGCTCACAATAAATGTCGTAACCTTAAAGACCTTGTGGCGACACACTGTTGTAAGGCACAGGTGACTTTTGTTGTGCTTGCTATAGCTCTTGGTGTGACAGTTTGGAAACTCGTCAAACAGGTTTTGTTTAAAGTGGCTGGTCTTTTTACAGCCGTTGTGGATTTTTGTAGTAAGGCTTGGAATGGGTTTTGTGACCAACTAATCCATGCCAAACTCTTTGTGAATGAATTGGGCTGTGTTTTGGTTGGTGTTAAAAACCACTGTTTTCAACTCTTGCTTGTAGCAGTCCATGCTTTTTATAAAACCTTGGAAAAGTGTGCCATAGGTAGAATTTGGGCCGGTGATCTACTTTTTTGGGCTGGTGGAGCTCACAAAGTGGAGGAAGACAATGGTGAAGTTTGGTTTGACGCTGTATACACTGTACAAGTTGACGAACAAGGCACTGTCATTGAAGACAACGTGCCTGACTTTGTTGTTTGTCATGATGTGTCTATACCTGAGCGTCAAAAGGGATATATAGTGCAGGTTTCTGCTGATGGTAAAAATTATATGTTTTTTAGGTACCAGCAGGGTGTTAACACCTATTATGCCCCAATGTCTCAGGTTGGTGCAGTTAATGTTGTTTGTAAGGCAGGTGGTAAAACTGTGACTTTTGGTGAAGACCAGGTCAAAGAGCTACCAACTCCAGATGTTAAGTGTATTTATCTTGATATTGAGTGTTGTGGTGAACCTTGGACTAGTGTCTTTAAGCGTGTTTATAAAGACCCAGTTGAAGTTGAGACATCTTTGACTGTAGAGGAGTTGCGAGCAGTTGTCTATGAGATGATGTGTGACAGTTTAAAACTATTTCCAGATGCTCCAAAACCTCCGCATTATGATAATGTTGCCTTGGTTGATAACCAGGGTCGTGATTTGCAAACCATTGATTCTTGTCATTTGGTTTATGTGGATTATGACAGTGATGGTGCAGTTTCTGAAGAAGAAGAAGAAGATGTGTCAGACACTGAAGATGTCAATGAGGAAGACGAACGATTGGCTGGTTTGCTTAAAAACCCTGCCAACTTTAAATATCCTCTTCCTTATGATGATGAATATAGTGTTTTTTGTGGGCGTCTCGTTCACAAATGTGCTATAGACATTTATCATTATCCATCTGGTGATGATATGTATGTCATTAATGATGCTTTTGATGGTGCCGTTAAGGCAATTCCTCAGAGTGTTGTTGATGTTTTAGGAGATTGGGCTGCTGCTGTTGACAGTCAAGAGCGCGCTCTACTCGTAGAAACTCCTGTTGAGACTCCTAAGGAAGAAACTCAGAAGCCTCAAAAGGTGGAAGAGCAGAAACCTAAGGAAACTCCTGTTGAGACTCCTAAGGAAGAAACTCAGAAGCCTCAAAAGGTGGAAGAGCAGAAACCTAAGGAAACTCCTGTTGAGACTCCTAAGGAAGAAATTCAGAAGCCTCAAAAGGTGGAAGAGCAGAAACCTAAGGAAACTCCTGTTGAGACTCCTAAGGAAGAAACTCAGAAGCCTCAAAAGGTGGAAGAGCAGAAACCTAAGGAAACTCCTGTTGAGACTCCTAAGGAAGAAACTCAGAAGCCTCAAAAGGTGGAAGAGCGAAAACCCAAGGAGACTCTTTTAAATAAACAATTTCTCCCTGATAGTTCGTCTGATGATGAACCAAGGAAGAAGTCTTTTAGATTTAAATTGAAACCTACAAAATGTAAAGTGCCCAATAATGTTGAGTATTCTACATGTGTGGGTGATTTGTCTGTAGTCGTTGCTAAGGCAATGGACTCCTATGAGGATTTTGTTTTGGTTAATGCTGCTAATGAGCACATGCGTCATGCCGGTGGTGTGGCACAGGCCATAGCAGACTTTTGTGGTAATAAGTTTGTAGATTATTGTGACACTTTTATTAGTAAGAATGGGCCCCAACAGCAATTGTTAGCCCCTTCTGGTGTTGCGAAGATACAAGCAGTTAATAATGTTGTTGGGCCTAGGCATGGGCAGCATGACTTGTTTGATAAGCTTGTTGCTGCATACAAGAAGGTTGTTGTTCCTGGTGCCATTAACTATGTGGTGCCGGTCTTGTCGGCAGGTATTTTTGGTGTGGATTACAAAATGTCTATTGATGCAATGCGCAAGGCATTTTGTGACCTTAAAATTCGTGTGCTCTTGTTTTCTCTTAATCAAGAACACATTGAATATTTTAATGCCACTTGTAAACAGAAGGGTGTTTACCTGACGGAAGATGGCACGTCTTTTAAAACTCTTGTTTTGAAACCAGGTGACACATTGGGTCACCTCGGAGGTGTTTTTGCAAAGAACAAGACAGTTTTTACAGCAGATGATGTTGAGGACGAAGAAGTTCTCTTCACTCCAACAACGGACAAAGCTGTTCTTGAATACTATGGATTGGATGCGCAAAAGTATGTAATATATTTGCAAACACTTTCGCAGAAATGGGAAGTCCAATATAGGGACAATTTTATCTTACTTAAATGGCGTGATGGAAACTGCTGGATTAGTTCAGCTATGGTGCTCCTTCAGGCCGCTAAGATTAGGTTTAGAGGTTTTCTTGCAGAGGCATGGGCTAAATTCTTGGGTGGAGACCCTACGGATTTTGTTGCTTGGTGCTATGCAAGTTGCAATGCTAAAGTTGGTGATTTTTCAGACTGCAATTGGCTTTTGGCTAACTTGGCAGAACACTTTGATGCAGATTACACAAATGCACTGCTTAAGAAGCGTGTGTCGTGTAACTGTGGTGTTAAGAGTTATGAACTTAGAGGCCTTGAAGCTTGTATTCAACCAGTGAGGGCTCCTAACCTTCTACATTTTAAGACGCAATACTCAAATTGTCCAACTTGTGGTGCAAATAGTATGGATGAAGTTGTTGAAGCATCACTCCCGTACTTGTTGCTTGTTGCTACTGATGGTCCTGCTGCAGTTGATTGTGATGAAAATGCTGCAGGAAATGTTGTTTTTATTGGGTCCACCAACAGTGGACACTGTTATACACAAGCTGTTGGAAAAGCTTTTGATAATTTGGCTCAAGATAGGAAATTTGGAAAGAGATCGCCTTACATTACTGCAATGTATATGCGCTTCTCTCTTAAGAGTAAAAATTCTCTGTCTGTTGCTAAAAAGAGTAAGAGCAAGAGTGATGTAGTAAAAGAAGATGTTTCTAATCTCGCTACAAGTTCAAAATTCAGTTTTGATGATCTTACTGATTTTGAACAGTGGTATGATAGTAACATCTATGAAAGTCTTAAAGTGCAGGAAACACCTGAGAATATGGATGAGTATGTGTCATTCACAACAAAGGAAGATTCCAAGTTGCCACTGACACTTAAAGTTAGAGGTATCAAATCAGTTGTCGACTTTAAGTCAAAGGATGGCTTTACTTATAAGTTAATACCTGACACTGATGAAAATTCAAAAGCACCAGTTTACTATCCTACCTTGGACGCTATAAGTCTTAAGGCAATATGGGTGGAAGGTAGTGCTAACTTTATCGTTGGACATCCCAACTATAATAGTAAAGTTCTGCGTGTTCCTACATTCTGGGAGTGTGCAGAGAGCTTTGTTAAAATAGGCGAAAAAGTTGATGGTGTAACTATGGGCCTCTGGCGTGCAGAACACCTTAATAGACCTAATTTGGAAAGAATTTTCAACGTCGTTAAGAAGACGATGGTTGGAACTAGTGTTGTTACTACACAATGTGGTAAATTAATTAGTAAAGCAGCTACATTCGTTGCCGATAAAGTAGGAGATGGTGTAGTTCGCAATGTTTCTGATAGAATTAAAGGGTGTTTTGGGTCTACTAGGGAGCATTTTGAAAGAAGAGTGTCTCCGCAGTTCTTAAAAACCCTCTTTTTCTTCGTCTTTTGTTTTTTGAAGGCTAGTGTTAAGGGTTTAATGGCTAGCTACAAGAGTGTGTTATGTAAAGTGTTATTTACTGCTTTACTTATATTGTGGATTGTCTACACTAGCGACCCAGTTATATCTACTGGAATACGTGTGTTAGATTTCCTATTTGAAGGTTCATTTTGTAGTCCTTATGCGGATTATGGTAAAGAGTCTTTTGATGTGTTACGCTATTGTGGAAGTGACTTTACTCGTCGTGTTCGTTTACATGGTAAGGATTCTCCTCATTTGTATAAACATGCCTATAGCGTAGCACAGTTTTATAAAGATGCAGTTAATGGTATCAGTTTTACATGGAATTGGCTTTATATGCTGTTTCTACTATTGTTTGTTAAGCCAGTAGCAGGTTTTGTTATTATTTGCTACTGTATAAGGTACTTGGTTTTGAGCACAACTGTTTTACAAACTGGTGTAGGATTCTTAGACTGGTTTATCCAAACAGTGTTTGCTAACTTTAATTTTATGGGTGCAGGATTTTATTTCTGGCTTTTTTATAAGGTATATATACAGGTGCATCATATTATGTACTGTAAGGATATAACATGTGAAGTGTGTAAGCGAGTTGCACGTAGTAATAGACATGAGGTTAGTGTTGTTGTTAACGGGCGTAAACAGCTTGTTCACGTCTACACTAACTCAGGCTATAGTTTCTGTAAGAGACATAATTGGTATTGTAGAAATTGTGATAAGTATGGCCACCAAAATACATTTATGTCTCCTGAAGTTGCTGGCGAGCTTTCTGAAAAGCTTAAGCGCCATGTTAAACCTACAGCATTCGCATACCACGTAGTGGATGATGCATGCTTAGTTGATGACTTTGTTAATTTGAAGTATAATGCTGCCACTCCAGGTAAAGAAGGTGTGCATTCTGCTGTTAAATGTTTTAGTGTATCTGATTTCTTAAAGAAAGCTGTTTTTCTTAAGGATGCACAAAAATGTGAGCAAATATCTAATGATAGTTTTATAGTTTGTAATACACACAGTGCGCATGCATTAGAGGAAGCAAAAAATGCTGCCATCTATTATGCGCAATGTCTCTGTAAACCAATTCTTATACTTGACCAAGTGCTTTATGACACTTTGGCTTGTGAACCTGTGTCTAAGAGTCTTGTTGACAAAGTGTGTGGTGTTTTGGCCGGCATCCTTGATGTTGATAATTACTGTTTTTCTGGTAAACTCAATTATAAAGCCGGTTCTCTTCGTGATGCCCTCTTGTCTGTTACGGCAGGTGAAGAGGCTGTTGATATGGCTATGTTTTGTCATAATTATGACCTTAGTTACACTAGTGATGGTTTTACTAATGTTGTTCCTTCTTATGGGTTTGACCTTGGTAAACTGACACCACGTGATAAAGGTTTTCTTATAAATGCTGATGCGTGCATAGCTAATCTTAAGGTTAGAAATGCACCACCTGTGGTGTGGAATTATCATGACCTTATAAAGTTGTCTGATAATTGTCTTAAGTTTTTAGTGTCTGGTGTTATAAAATCAGGAGCTAAATTTTATGTAACACGTTCTGATGTTAAACAAGTCATTACTTGTCTAACACAGAAGTTGTTGTTAGATAAGAAGGCCGGTGGTGTTCTTAAGGATACTATGTCTTGGTTTTGGTTTATAGTTAAATTTGTTGTTGTGTGTTATCTTATTTTTACAGCAGGTTGTTTGTATGTTTATCATACAGATTATTATGGTGTTAAGATGCACCCGATGTATGATGTGAATTCAACCTTTCCTGTTGAGAATTTTAAGGTTATAGAAAATGGTGTCCTTAGAGACCTGGTTTCAGAGGACAATTGTTTTTCAAATAAGTTTTTGGGTTTTGATGACTTTTGGGGCAAACCTTATACTAATAGTAGGGATTGTCCAATAGTTACTGCTCTTATAGATGGTACCGGTTTTGTTGCGGCAGGTGTTCCTGGTTATGTTCATTGGGTTATGGACAATACCATGTTTATACACACTGCACAAGCAGAACAAAAACCTTGGTATGCACCTAGTTGGTTCCCCCAGGAGGATGTAGTTGGGTATACTATTGACTCTGTTATTACACAGGGTGAATTTTATAGCTCAATTGCGACGTTTCCTGCACGTTGTATGTATTTTGCAACAAGTGCGGCGCCTCAATTGTATTGTTATGGTGGTGAAAATGATGCTCCAGGTGCTATGCCATATGAGAGTGTTCAACCTCATCGTGATATTTTCCAACCAAATAATGTTAGGTTTTCTATTCCTCAACAAATTATGTACACACCCTATATAGTTAAGTTGGCTTCTGACAACTATTGTAGAGGTAGTGTTTGTGAAAAGACAAAGCTTGGTTATTGTTATTCATGGAACCCTCGTTGGGTTCTTTATAACGATGACTATGTTAAGTTGCCTGGTGTTTATTGTGGGGCCACTGTTAGAGAACTTGTGTTTTCTATGTTGGGCTCATTTTTTACAGGCGTTAGCCCTAATTTGTATTTGCACCTAACAACTATGTTTTTGGTATTGTTGTGTGTTATGGTTGTGTTTGCACTGGTTATTAGGTTTCAAGGTGTTTTTAAAGCCTATACACCAGTCGTTTTTGCAGTCATTATGGTTTGGGGCCTGAATGTCTTTATGTTGTGTGTCTATAGCTATAACCCTTTTGTAGCGGTTATGTTGTTGGCACTTTATTGTTATATGTCACTCATGGTTGGACGTACCGTTTCTACGGTTATGCACCTGTGGGTTGTATTTACTTTTATAATGGTTGTACCTTGGTGGTTGGCCTGTGTTTATATAGCTTTTATTGCTTATATGTACACTCCATTTGCCATGTGGTGTTATGGCACTGCCAAACGAACTCGTAAGCTTTACGAAGGTAACGAGTTTGTAGGCAGTTATGATTTGGCAGCCCAGAGTACGTTTGTTATACGTAATGTTGAGTTTGTCAAACTTTCTAATGAAGTCGGTGAAAAGCTTGACGTTTATTTGTCAGCCTATGCACGACTAAAGTATTATTCAGGCACTGGCGGTGAGCAAGACTATTTGCATGCTTGTCGTGCCTGGTTGGCTTATGCTTTGGATCAATACCGTGCTAATGGTGTTGAAGTGTTGTATACACCACCTAAGTTTTCAATGGGTGTTAGTAGGTTGCAAGCAGGCTTTAAGAAGTTTGTTTCTCCTAGTAGTGCCGTTGAGAAGTGCATTGTTAGTGTATCCTATAGGGGTAATAATCTTAATGGATTGTGGTTGGGTGACTCCATCTACTGTCCACGTCATGTGTTAGGTAAGTTTAGCGGTGACCAATGGGGTGATGTACTTAATCTTGCTAATAATCATGAGTTTGAAGTAGTAACTGGAAATGGTGTTACTTTGAGTGTTGTCAGTAGGCGTTTGAAAGGTGCAGTTTTAATTTTACAAACTGCAATTGTCAATGCTGATACACCGAAGTATAAATTTTTGAAAGCAAATTGTGGTGATAGTTTCACAATAGCTTGCTCTTATGGTGGTATAGTTATAGGACTCTACCCTGTTACTATGCGTTCTAATGGAACTATTAGAGCATCTTTTCTTGCTGGAGCATGTGGTTCAGTAGGTTTTAATATAGAAAAGGGTGTAGTAAATTTTTACTACATGCACCATCTAGAGTTACCTAATGCATTACACACAGGAACTGACCTAATGGGTGAGTTTTATGGTGGTTATATAGATGAAGAGGTTGCTCAGAAAGTTCAACCCGATAAATTAGTTACTAATAATATTTTGGCATGGCTTTATGCAGCAATTATTAGTGTTAGAGAGAGTAGTTTTTCAACACCAAAATGGCTTGAAAGTACTACTGTCAGTATTGAAGATTATAATAAGTGGGCAGTGGATAATGGTTTTACATCATTTGTAAGCTGCACTGCTATTACTAAATTAAGTGCTATAACAGGAGTTGATGTTTGTAAACTCCTTCGTACTATTATGGTAAAAAGTGCACAATGGGGTAGTGAACCTATTTTGGGACAATATAATTTTGAGGATGAAATGACACCGGAATCTGTTTTTAATCATGTGGGTGGTGTTAGGTTACAATCTTCTGTTGTAAAGAGAGCCGCATCTTGGTTTTGGAGTACATGTGTGCTATTTTGTTGCTTGTTTGTCTTGTGTTCTATAGTTCTGTTTACAGCTATACCATATAGATATTATTTACATGGTGCTGTTGTTTTATTTGCAACTGTGCTCTTTGTGTCGTTTACTGTGAAACATGTTATGGCATATATGGATACCTTCCTTTTGCCGACATTGATTACGGTTATTATAGGGGTTTGTGCTGAAGTGCCTTTCATCTATAATACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTAGTCTTTGACACTGTAGTACCGTGGATGTTTCTACCATTAGTCTTGTACACAGCTTTTAAATGTATACAAGGTTGTTATAGTGTAAATTCCTTTAATACTTCTCTGTTAGTGTTGTATCAGTTTATGAAGTTGGGTTTTGTTATATATACCTCTTCTAATACTCTTACAGCATATTCAGAAGGTAACTGGGAACTATTCTTTGAGTTAGTGCACACAACTGTGTTGGCTAATGTTAGTAGTAATTCTTTAATTGGCTTAATTGTGTTTAAATTTGCTAAGTGGATGCTGTATTATTGTAATGCTTCATACCTTAATAATTATGTTCTAATGGCTGTCATTATTAATGGCATAGGTTGGATGTTTACTTGTTACTTTGGATTCTATTGGTGGATTAATAAGGTTTTTGGTTTAACCTTAGGTAAATATAGTTTCAAAGTTTCAGTAGATCAATATAGGTATATGTGTCTTCATAAGATTAATCCACCTAAAACTGTGTGGGAAGTCTTTTCTACAAATATACTTATACAAGGTATAGGTGGTGACCGTGTGTTGCCTATAGCTACAGTGCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTCTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTAGGGTTACACAATAAAATTCTTGCCTCTGATGATGTTAATGAGTGCATGGACAATTTGTTGGGTATGCTTGTAACACTATTCTGTGTAGATAGTACTATCGATTTAAGTGAGTATTGTGATGATATACTTAAGAGGTCAACTGTTTTACAATCAGTTACTCAGGAGTTTTCACACATTCCCTCCTATGCGGAGTACGAAAGAGCTAAAGATCTTTATGAAAAGGTTTTAGCTGATTCTAAAAATGGTAGTGTTACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCGGTTTTTGATAGAGATCTGGCTGTTCAGAAAAAGTTAGATAGCATGGCCGAACGTGCTATGACAACAATGTATAAAGAAGCGCGTGTTACTGATAGAAGGGCGAAACTTGTTTCATCATTACATGCGCTATTATTTTCAATGCTTAAGAAGATAGATTCTGAAAAGCTCAATGTACTATTTGATCAAGCAAGCAGTGGTGTTGTACCGCTGGCTACTGTTCCAATAGTTTGTAGTAATAAGCTTACTCTTGTAGTGCCAGACCCAGAAACATGGGTCAAGTGCGTAGAGGGCATGCTTGTTACTTATTCCACAGTTGTTTGGAATATTGATACTGTTTTTGATGCTGATGGTGTTGAAATTCAACCTAGTTCTAACGGGCAAAGTCTTGTCTACCACATAGGTGGTGACAGAATTGCTTGGCCACTTAAAGTTAATCTTACGCGTAATGTTCATAATAAAGTGGAGGCTTCTCTGCAGAATAATGAACTTATGCCCCAAGGGATAAAGACTAAGGCTTGTATTGCAGGGACTGATCAAGCTCATTGTAATGTAGAGTCTAAGTGTTATTATACTAATATTAATGGTAGTTCTGTTGTAGCTGCCATTACATCTGATAATCCAAATTTGAAAGTTGCATCATTCTTGAGTGATGCTGGCAATCAAATTTTTGTTGATCTTGACCCGCCATGTAAATTTGGTATGAAAGTTGGTGGTAAGGTTGAGGTTGTTTATCTTTATTTTCTTAAAAATACAAAGTCTATTATTAGAGGTATGGTTCTTGGAGCCATTTCCAATGTTGTTGTATTGCAATCTAAAGGACATGAAACTGAAGAGCTTGAGTCTGTTGGTATCTTGTCACTTTGTGCTTTTGCTGTGAATCCTGAAGAGACGTATTTGAAATATGTGTCTGCAGGAAATCAACCACTTGGAAACTGTGTTAAGATGTTGACTGTGCATAATGGTAATGGTTTTGCCATAACTACTAAACCCAGTCCGACACCTGACCAAGATTCTTATGGTGGTGCTTCTGTGTGTTTGTATTGTAGAGCACATATAGCACACCCAGGAGCTTCTGGTACTTTGGATGGTCGTTGTCCTTTTAAAGGTTCTTTTGTTCAAATACCTACTTCTGAAAAAGACCCTGTTGGTTTTTGTCTACGAAATAAAGTTTGTAAAGTCTGTCAGTGTTGGGTAGGTTTTGGCTGTCAGTGTGATTCACTTAGACAACCAAAACCCTCAGTTCAATCAGATGCTGGTGCACCTGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MAPGLSKGVSPNKKNVIFVVKDIQPALCDALFFYTSHVPRDFADAHTVRERFDRNLQSGRVFKFETVCGPVYLQGVPSVPPGVKALDAESKLVDLENIFGVSPLSRRYRDLLKTAPQWSLNVESLDAQAEKLDTLFSSSEILWLQVAAKVQVSAMALRRLVGEVTAKVMDSLGSNLSALFQVVKETMARIFQKALAIFESVSELPQRIAALKMAFAKCARSITVVVVGHALTIREFAGTCLASINGAVAKFFEELPTGFMGAKVFSTLAFFKEAAVKIVENIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEVPVEPESWSAILEGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVLLSDGYTPEINDGLLLAALYTSSTVADVLAALKKGEPFKFLGHSFVYVKDATVSFTLAKGASIADVLKLFVKEAADVWSVFNEKSHEFWTRAHNKCRNLKDLVATHCCKAQVTFVVLAIALGVTVWKLVKQVLFKVAGLFTAVVDFCSKAWNGFCDQLIHAKLFVNELGCVLVGVKNHCFQLLLVAVHAFYKTLEKCAIGRIWAGDLLFWAGGAHKVEEDNGEVWFDAVYTVQVDEQGTVIEDNVPDFVVCHDVSIPERQKGYIVQVSADGKNYMFFRYQQGVNTYYAPMSQVGAVNVVCKAGGKTVTFGEDQVKELPTPDVKCIYLDIECCGEPWTSVFKRVYKDPVEVETSLTVEELRAVVYEMMCDSLKLFPDAPKPPHYDNVALVDNQGRDLQTIDSCHLVYVDYDSDGAVSEEEEEDVSDTEDVNEEDERLAGLLKNPANFKYPLPYDDEYSVFCGRLVHKCAIDIYHYPSGDDMYVINDAFDGAVKAIPQSVVDVLGDWAAAVDSQERALLVETPVETPKEETQKPQKVEEQKPKETPVETPKEETQKPQKVEEQKPKETPVETPKEEIQKPQKVEEQKPKETPVETPKEETQKPQKVEEQKPKETPVETPKEETQKPQKVEERKPKETLLNKQFLPDSSSDDEPRKKSFRFKLKPTKCKVPNNVEYSTCVGDLSVVVAKAMDSYEDFVLVNAANEHMRHAGGVAQAIADFCGNKFVDYCDTFISKNGPQQQLLAPSGVAKIQAVNNVVGPRHGQHDLFDKLVAAYKKVVVPGAINYVVPVLSAGIFGVDYKMSIDAMRKAFCDLKIRVLLFSLNQEHIEYFNATCKQKGVYLTEDGTSFKTLVLKPGDTLGHLGGVFAKNKTVFTADDVEDEEVLFTPTTDKAVLEYYGLDAQKYVIYLQTLSQKWEVQYRDNFILLKWRDGNCWISSAMVLLQAAKIRFRGFLAEAWAKFLGGDPTDFVAWCYASCNAKVGDFSDCNWLLANLAEHFDADYTNALLKKRVSCNCGVKSYELRGLEACIQPVRAPNLLHFKTQYSNCPTCGANSMDEVVEASLPYLLLVATDGPAAVDCDENAAGNVVFIGSTNSGHCYTQAVGKAFDNLAQDRKFGKRSPYITAMYMRFSLKSKNSLSVAKKSKSKSDVVKEDVSNLATSSKFSFDDLTDFEQWYDSNIYESLKVQETPENMDEYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLIPDTDENSKAPVYYPTLDAISLKAIWVEGSANFIVGHPNYNSKVLRVPTFWECAESFVKIGEKVDGVTMGLWRAEHLNRPNLERIFNVVKKTMVGTSVVTTQCGKLISKAATFVADKVGDGVVRNVSDRIKGCFGSTREHFERRVSPQFLKTLFFFVFCFLKASVKGLMASYKSVLCKVLFTALLILWIVYTSDPVISTGIRVLDFLFEGSFCSPYADYGKESFDVLRYCGSDFTRRVRLHGKDSPHLYKHAYSVAQFYKDAVNGISFTWNWLYMLFLLLFVKPVAGFVIICYCIRYLVLSTTVLQTGVGFLDWFIQTVFANFNFMGAGFYFWLFYKVYIQVHHIMYCKDITCEVCKRVARSNRHEVSVVVNGRKQLVHVYTNSGYSFCKRHNWYCRNCDKYGHQNTFMSPEVAGELSEKLKRHVKPTAFAYHVVDDACLVDDFVNLKYNAATPGKEGVHSAVKCFSVSDFLKKAVFLKDAQKCEQISNDSFIVCNTHSAHALEEAKNAAIYYAQCLCKPILILDQVLYDTLACEPVSKSLVDKVCGVLAGILDVDNYCFSGKLNYKAGSLRDALLSVTAGEEAVDMAMFCHNYDLSYTSDGFTNVVPSYGFDLGKLTPRDKGFLINADACIANLKVRNAPPVVWNYHDLIKLSDNCLKFLVSGVIKSGAKFYVTRSDVKQVITCLTQKLLLDKKAGGVLKDTMSWFWFIVKFVVVCYLIFTAGCLYVYHTDYYGVKMHPMYDVNSTFPVENFKVIENGVLRDLVSEDNCFSNKFLGFDDFWGKPYTNSRDCPIVTALIDGTGFVAAGVPGYVHWVMDNTMFIHTAQAEQKPWYAPSWFPQEDVVGYTIDSVITQGEFYSSIATFPARCMYFATSAAPQLYCYGGENDAPGAMPYESVQPHRDIFQPNNVRFSIPQQIMYTPYIVKLASDNYCRGSVCEKTKLGYCYSWNPRWVLYNDDYVKLPGVYCGATVRELVFSMLGSFFTGVSPNLYLHLTTMFLVLLCVMVVFALVIRFQGVFKAYTPVVFAVIMVWGLNVFMLCVYSYNPFVAVMLLALYCYMSLMVGRTVSTVMHLWVVFTFIMVVPWWLACVYIAFIAYMYTPFAMWCYGTAKRTRKLYEGNEFVGSYDLAAQSTFVIRNVEFVKLSNEVGEKLDVYLSAYARLKYYSGTGGEQDYLHACRAWLAYALDQYRANGVEVLYTPPKFSMGVSRLQAGFKKFVSPSSAVEKCIVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWGDVLNLANNHEFEVVTGNGVTLSVVSRRLKGAVLILQTAIVNADTPKYKFLKANCGDSFTIACSYGGIVIGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLMGEFYGGYIDEEVAQKVQPDKLVTNNILAWLYAAIISVRESSFSTPKWLESTTVSIEDYNKWAVDNGFTSFVSCTAITKLSAITGVDVCKLLRTIMVKSAQWGSEPILGQYNFEDEMTPESVFNHVGGVRLQSSVVKRAASWFWSTCVLFCCLFVLCSIVLFTAIPYRYYLHGAVVLFATVLFVSFTVKHVMAYMDTFLLPTLITVIIGVCAEVPFIYNTLISQIVIFFSQWYDPVVFDTVVPWMFLPLVLYTAFKCIQGCYSVNSFNTSLLVLYQFMKLGFVIYTSSNTLTAYSEGNWELFFELVHTTVLANVSSNSLIGLIVFKFAKWMLYYCNASYLNNYVLMAVIINGIGWMFTCYFGFYWWINKVFGLTLGKYSFKVSVDQYRYMCLHKINPPKTVWEVFSTNILIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVGLHNKILASDDVNECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKDLYEKVLADSKNGSVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWVKCVEGMLVTYSTVVWNIDTVFDADGVEIQPSSNGQSLVYHIGGDRIAWPLKVNLTRNVHNKVEASLQNNELMPQGIKTKACIAGTDQAHCNVESKCYYTNINGSSVVAAITSDNPNLKVASFLSDAGNQIFVDLDPPCKFGMKVGGKVEVVYLYFLKNTKSIIRGMVLGAISNVVVLQSKGHETEELESVGILSLCAFAVNPEETYLKYVSAGNQPLGNCVKMLTVHNGNGFAITTKPSPTPDQDSYGGASVCLYCRAHIAHPGASGTLDGRCPFKGSFVQIPTSEKDPVGFCLRNKVCKVCQCWVGFGCQCDSLRQPKPSVQSDAGAPGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
2Q6F
E-value=0.0 Score= 592 Identity=91.23%
Cov(Q)=7.74% Cov(P)=99.68%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3981
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
412.813670000001
Exp number, first 60 AAs:
0.00429
Total prob of N-in:
0.00245
outside
1 - 1763
TMhelix
1764 - 1786
inside
1787 - 1872
TMhelix
1873 - 1895
outside
1896 - 1909
TMhelix
1910 - 1932
inside
1933 - 2304
TMhelix
2305 - 2327
outside
2328 - 2389
TMhelix
2390 - 2412
inside
2413 - 2586
TMhelix
2587 - 2609
outside
2610 - 2613
TMhelix
2614 - 2636
inside
2637 - 2637
TMhelix
2638 - 2660
outside
2661 - 2669
TMhelix
2670 - 2692
inside
2693 - 3124
TMhelix
3125 - 3147
outside
3148 - 3151
TMhelix
3152 - 3169
inside
3170 - 3181
TMhelix
3182 - 3204
outside
3205 - 3218
TMhelix
3219 - 3241
inside
3242 - 3253
TMhelix
3254 - 3276
outside
3277 - 3285
TMhelix
3286 - 3308
inside
3309 - 3327
TMhelix
3328 - 3350
outside
3351 - 3981
