Strain
Avian_KM586818
(Region: China; Strain: Infectious bronchitis virus isolate P100, complete genome.; Date: 8-Aug)
Gene
1a
Description
Annotated in NCBI,
1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTTCAAGCCTAAAACAGGGAGCATCTTCCCAAAACCAGAGGAGTGTCATTCTTGTTGCCAAAGACATTCCCGACCAACTTTGTGACGCTTTGTTTTTCTATACGTCACACAACCCAAAAGACTACGCCGATGCTTTCGCCTTTAGGCAGAAGTTCGATTGTAGTCTCGGTGCTGGGAAACAGTTTAAATTTGAAACTGTGTGTGGTCCATTCTTTTTGAAGGGAGTGGATAAAATAACACCAGGAGTCCCAGCCAAAGTTATAAAAGCCACTTCAAAGTTGGCTGACTTAGAAGACATCTTTGGTGTCTCACCTCTTGCACGGAAATACCGTGAACTCTTGAAAACAGCATGCCAGTGGTCTCTCACTGTAGAAGCGCTGAATGCTCGTGCACAAACTCTTGCAGAAATCTTTGAACCAACTGAAATATTTTGGCTTCAAGTGGCTGCAAATATTCAGGTTTCAGCCATGGCAATGCGCAGACTTGTTGGTGAGGTCACTGCAAAAGTCATGGATTCACTTGGCTCAAGTTTGAGCGCTCTATTCCACGTTGTTAAGGAAACAATGACCAGAATCTTTCAAAAGGCTTTGGCCATTTTTGAGAGTGTGAGTGAATTGCCACAGCGTATTGCTGCACTTAAAATGGCTTTTGCCAAGTGTGCAAGATCAATTACAGTTGTGGTTGTTGGACATGCTCTCACTATTAGAGAGTTTGCTGGAACCTGTCTTGCAAGCATTAATGGTGCAGTTGCAAAATTCTTTGAAGAACTTCCAACTGGCTTTATGGGCGCTAAGGTCTTTTCAACTCTTGCCTTCTTTAAAGAAGCAGCAGTAAAGATCGTAGAAAATATACCAAACGCGCCAAGAGGTACTAAAGGTTTTGAAATCGTTGGCAATGCCAAAGGTACACAAGTTGTTGTCCGTGGCATGCGAAATGACTTAACTCTGCTTGACCAAAAAGCTGAAGTTTCTGTGGAACCAGAAGGTTGGTCTGCAATTTTGGAGGGACATCTTTGCTATGTCTTTAAGAGTGGTGATCGCTTTTATGCGGCACCTCTTTCAGGAAATTTTGCATTGCATGATGTGCATTGCTGCGAGCGTGTAGTCTGTCTGTCTGATGGTGTAACACCGGAGATAAATGATGGGCTTGTTCTTGCTGCAATTTACTCATCTTTTAGTGTTTCGGAACTTGTGGCAGCACTTAAAAAAGGTGAACCATTCAAGTTCTTGGGGCACAAATTTGTTTATGCGAAGGAAGCGGCAGTTTCTTTCACTTTGGCAAAAGCAGCAACTATTGGAGATGTCCTGAAGCTGTTTCAATCAGCAAGAGTAAAGACGAAAGATGTGTGGTCTGCACTTACTGAACAGTCTTTTGAATTCTGGAAACTTGCGTACAATAGAGTGCGCAATCTTGAAGAAGTTCTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTGTTGTCCTAGCAGTAGTGCTTGGTGAGGGCATTTGGCATTTCTGTTCACAAGTCATTTATAAATTGAGTGGCTTGTTTTCCAAAGTTGTTGACTTTTGTGAAAAACACTGGAAAGGTTTCTGCACACAGTTGCGAAAAGCTAGGCTTGTTGTTACAGAAAACCTCTGTGTGTTGAAAGGAATTGCTCAGCATTGTTTTCAACTTTTGCTGGAAGCAATACATTCTATGTACAAGAGTTTTAAGAAATGTGCACTTGGTAGGATTTCCGGTGACTTGTTCTTCTGGAAAGGAGGTGTGCATAAAATAATTCACGATGGCGATGAAATTTGGTTTGACGCCGTCGACACCATTGAAGTTGAAGATTTGGGTACCACCCAAGAAAAACTTATAGATTTTGAGGTTTGCGAGGATGTGATACTTCCAGAAAATCAACCTGGCCACATGGTTCAAATCCAAGATGATGGAAAGAACTATATGTTCTTCCGTTTTAAGAAGGATGAGAACATTTATTATACACCAATGTCACAACTTGGTGCTATTAATGTTATTTGCAAAGCGGGTGGCAAGACTGTCACGTTTGGTGAAACAACTGTAAAGGAAATACCACCACCTGATGTTGTGCCTATTAAGGTAAGCATTGAGTGTTGTGGTGAACCATGGAATACAATCTTCAAGAAGGCTTATAAAGAGCCCATTGAAGTTGAGACAGATCTCACAGTAGAACAATTGCTCACTGTGATTTATGATAAAATGTGTGAAGATCTCAAACTGTTTTCAGAGGCACCAGAACCTCCACCATTTGAGAATGTCGCACTTGTTGATAAAAATGGTAAAGATCTGGATTGCATAAAATCATGCCATCTCATCTACCGTGATTATGAGAGCGATGATGACATCGAGGAAGAAGATGCTGAGGAGTGTGATACAGATCCAGCTGATGCTGAGGAATGTGATACTGCTTCAGAGTGTGAAGAAGAAGATGAGGATACAAAGGTGCTGTCTCTTTTGCAAGACCCAGCCAGTAACAAGTATCCTCTACCTCTTGATGATGATTATAGTGTCTATAATGGATGTATTGTTCATAAGGACGCTCTAGATGTTGTTAACTTACCATCTGGTGAAGAAACTTTTGTTGTCAATAACTGTTTTGAAGGGGCTGTGAAACCGCTTCCGCGGAAGGTTGTTGATGTCCTTGGAGATTGGGGAGAGGCCGTTGATGCACAAGAACAATTGTGTGAACAAGAGTCTGAATCTGTTAGTGGTTCAGCAATTGAAGACGCTGTTGTTGTAGAGCAGGAAGTAGTTCCTGTTGTTGAAGAGTCTCAAGAAATAGTTGTTTTTACACCTGCTGATTTAGAAGTTGCTAAAGAAACATCAGAGGATGTTGACGAGTTTGTTCTTGTTGCTGATGTTCCTACAGAAGAAGTTGTGCCTCTAGAAAAAGAGGAGTCACAAGTAGAGCAGGGGCCTATTCAAGTTGTCAAATCACAACGTGAGAAAAAGGCTAAAAAGTTCAAAGTCAGGCAAACTACATGTGAAAAACCCAAATTTTTGGAGTATACAACATGTGTAGGTGATTTGACTGTTGTAATTGCCAAAGCATTGGATGAATTTAAAGAGTTCTGCGTTGTAAACGCCGCAAATGAGCATATGTCTCATGGTGGTGGCGTTGCAAAGGCAATTGCAGACTTTTGTGGACCTGATTTTGTGGAATATTGTGAGGATTATGTTAAGAAACATGGGCCACAACAAAAACTTGTCACACCTTCGTATGTTAAAGGCATTCAATGTGTGAATAATGTTGTAGGACCCCGCCATGGTGACAAAAATTTGGAGGAAAAACTTGTTGCTGCCTACAAGAGTGTTCTTGTAGATGGTGTGGTCAATTATGTTGTGCCAGTTTTATCATCGGGAATTTTTGGTGTAGATTTTAAAACTTCAATAGACGCGATGCGTAAGGCCTTTGAAGGGCTTAACATACGCGTGCTTCTATTTTCTCTGAGTCAAGAACACATCGATTATTTCGATATAACTTGCAAGCAGAAGAAAATTTATCTTACAGAAGATGGTGTTAAATACCGCTCTGTTGTAGTGAAACCTGGCGACTCTTTGGGTCAATTTGGACAGGTTTTTGTAAAGAACAAGACAGTTTTTACAGCAGACGATGTTGAGGACGAAGAAGTCCTCTTCATTCCAACAACTGACAAAGCTGTTCTTGAATATTATGGACTAGATGCGCAAAAGTATGTAATATACTTACAAACACTTGCGCAGAAATGGGAAGTCCAATATAGAGACAATTTTATTTTACTTAAGTGGCGTGATGGAAATTGCTGGATTAGTTCATCAATTGTACTCCTTCAAGCTGCTAAGATTAGGTTTAAGGGTTTTCATGCAGAAGCATGGGCTAAATTGCTGGGTGGAGACCCCACCGAGTTTGTAGCTTGGTGTTATGCAAGTTGCAACGCTAAAGTTGGTGATTTTTCAGACTGCAATTGGCTGTTGGCTAATTTAGCAGAATACTTTGATGCAGACTACACGAATGCACTTCTTAAGAAGCGTGTTTCGTGTAACTGCGGTGTTAAGAGTTATGAATTTAGAGGCCTTGAAGCTTGTATTCAGCCGGTAAGAGCTCCTAATCTTCTACATTTTAAGACGCAATATTCAAATTGTCCAACATGTGGTGCAAATAGCACGGATGAAGTTGTTGAAGCTTCACTCCCGTACCTATTGCTCGTAGCTACTGATGGTCCTGCTGCAGTTGATTGTGATGAAAATGCTGCAGGAAGTGTTGTTTTCATTGGATCTACTAATAGTGGCCATTGTTATACTCAAGCTGCTGGAAAAGCCTTTGATAATTTGGCTCAAGATAGAAAATTTGGGAAGAGGTCGCCTTACATTACAGCAATGTACACGCGTTTCTCGCTTAAGAATGAAAATTCTCTGTCTATTGCTAAAATGAGCAAGAGTAAGAGCAAGGTAGTAAAAGAAGATGTTTCTAATCTCGCTACAAGTTCAAAATTCAGTTTTGATGATCTTACTGACTTTGAACAGTGGTATGATAGCAACATCTATGAAAGTCTTAAAGTGCAGGAAACACCTGAGAATATGGATGAGTATGTGTCATTCACAACAAAGGAAGATTCCAAGTTGCCACTGACACTTAAAGTTAGAGGTATCAAATCAGTTGTCGACTTTAAGTCAAAGGATGGCTTTACTTATAAGTTAATACCTGACACTGATGAAAATTCAAAAGCACCAGTTTACTATCCTACTTTGGACTCTATAAGTCTTAAGGCAATATGGGTGGAAGGTAGTGCTAACTTTATCGTTGGACATCCCAACTATAATAGTAAAGTTCTGCGTGTTCCTACATTCTGGGAGTGTGCAGAGAGCTTTGTTAAAATAGGCGAAAAAGTTGATGGTGTAACTATGGGCCTCTGGCGTGCAGAACACCTTAATAGACCTAATTTGGAAAGAATTTTCAACGTCGTTAAGAAGACGATAGTTGGAACTAGTGTTGTTACTACACAATGTGGTAAATTAATTAGTAAAGCAGCTACATTTGTTGCCGATAAATTAGGAGATGGTGTAGTTCGTAATGTTACTGATAGAATTAAAGGGTGTTTTGGGTTTACTAGGGATCACTTTGAAAGAAGAGTGTCTACACAGTTCTTTAAAACCCTCTTTTTCTTTCTTTTCTGTCTTGTGAAGGCTAGTGTTAAGGGTTTAATGGCTAGCTACAAGAGTGTGTTATGTAAAGTGTTATTTACTGCTTTACTTATAATATGGTTTGTTTACACTAGTAATCCAGTTATATTTACTGGAATACATGTGTTAGATTTCTTATTTGAAGGCTCATTCTGTAGTCCTTATGCGGATTACGGTAAAGAGTCTTTTGATATTTTACGCTATTGTGGAAGTGATTTTACTTGTCGTGTCTGTTTACATGGTAAGGATTCACTCCATTTGTATAAACATGCCTATAGCGTAGAACAGTTTTATAAAGATGCAGTTAATGGCATCAGTTTTACATGGAATTGGCTTTATATGCTGTTCTTAATATTGTTTGTTAAGCCAGTGGCAGGTTTTGTTATTATTTGCTACTGTATAAGATATTTGGTTTTAAGCACAACTGTATTACAAACTGGTGTAGGATTTTTAGACTGGTTTATTCAAACAGTGTTTGCTAACTTTAATTTTATGGGTGCGGGATTTTATTTCTGGCTCTTTTATAAGATATATATACAGGTGCATCATATAATGTACTGTAAGGATATAACATGTGAAGTGTGTAAGAGAGTTGCACTCAGTAATAGACGTGAAGTTAGTGTTGTTGTTAATGGGCGTAAACAGCTTGTTCACGTGTACACTAATTCTGGTTACACTTTCTGTAAGAAACATAATTGGTATTGTAAGAATTGTGACAAGTATGGTCACCAGAATACATTTATGTCTCCTGAAGTTGCTGGTGAGCTTTCAGAAAAGCTTAAACGCCATGTTAAGCCTACAGCACATGCATACCACGTAGTGGATGATGCATGTTTAGTTGATGACTTTGTTAATTTGAAGTACAATGCTGCCATCCCAGGTAAAGAAGGTGTGCATTCGGCTGTTAAATGTTTTAGTGTGTCTGATTTCTTAAAGAAAGCAGTCTTTCTTAAGGAAGCACAAAAATGTGAACAAATATCTAATGATAGTTTTATAGTTTGTAACACACACAGTGCGCACTCATTAGAGGAAGCAAAAAATGCAGCCATCTATTATGCGCAATGTCTTTGTAAACCAATTCTTATACTTGACCAGGCTCTTTATGAACAATTGACTGTAGAACCTGTGTCTAAGAGTGTTGTAGACAAAGTGTGTAGTATTTTGTCTAATATAATATCTGTAGACTCTGCAGCTTTGAATTATAAGGCCGGCACAATTCGTGATATCCTTCTCTCTGTTACTAAGGATGAAGAGGCTGTTGATATGGCTATCTTTTGTCATAATAATGATGTTGAATATACTAATGATGGTTTTACTAATATTGTGCCATCATATGGTATAGACACTGATAAGTTAACACCTAGAGATAGAGGCTTTTTGATAAGTGCAGATGCTGCCATTGCTAACTTGAAAGTGAAAAATTCACCGCCAGTAGTGTGGAAGTATTCTGATCTTGTTAAACTGTCTGACAGTTGTCTTAAGTATTTAATATCAGCAACTGTCAAATCAGGGGGTAGGTTTTTCATAACAAGGTCTGGTGCTAAACAAGTTATTGCTTGTTATACTCAGAAGTTGTTAGTGGAAAAGAAGGCCGGTGGTGTTGTTAGTAGCACTATTAGTTGGTTTAAGAGTTGTTTTAAATGGCTCTTAGTGTTTTATTTACTTTTTACAGCGTGTTGTTTGGGTTATTATCAATGGGAAATGAGTAGAGAATTTGCTCACCCCATGTATGATGTTAATTCCACAATGCATGTAGAAGGGTTTAAGGTTATAGATAAGGGTGTACTTCGGGATATTGTGCCAGAAGACACGTGCTTTTCTAATAAGTATGTTAATTTTGATTCGTTTTGGGGTAAACCTTATGTAAATAGTAGAGACTGTCCTATTGTAACCGCTATTATAGACGGTTCCGGTACAGTGGCTGCTGGTGTTCCTGGTTTTTTACAGTGGGTCATGGATGGTGCAATGTTTATCCATATGACACAGACTGAGCGAAGACCGTGGTACATTCCTACTTGGTTTAACAGAGAAATAGTTGGTTATACTCATGATTCTATTGTTACAGAAGGTGAGTTTTATACTTCCATAGCATTGTTTGCTGCCAGATGTTTGTATTTAACATCTAGTAACACACCACAGCTTTATTGCTTTAATGGTGATAGCGATGCTCCTGGTGCATTACCGTTTGCAAATATACTCCCTCATAGAGTATATTTTCAACCTAATGGAGTTAGGCTTATTGTTCCTCAGCAAATTATGCATACACCGTACATAGTTAGACTTGTTTCAGACAGCTATTGTAGAGGTAGTGTTTGTGAGTATACTAAACCAGGTTATTGTGTATCACTGAATTCCCAATGGGTTTTGTTTAATGACGAATACACAAGTAAACCAGGTGTGTTTTGTGGCTCTACTGTTAGAGAGCTTTTGTTTAGTATGGTTAGTACATTCTTTACGGGTGTTAATCCAAATATTTATATGCAATTGGCGACCATGTTCTTAATACTTGTTGCTGTTGTGCTAGTTTTTGCAATGGTTATAAAGTTTCAAGGTGTTTTCAAAGCTTATGCAACCACTGTATTTACTATAATGCTTGTTTGGCTTATTAATGCATTTGTATTATGTGTACATAGTTATAATAGTGTTTTAGCTATTATACTACTTGTGATTTATTGTTATGTATCACTTGTGACGAGTCGTAATACTGCTATAGTAATGCACTGTTGGCTTGTATTCACATTTGGTTTAATAGTACCTAATTGGATAGTTTGTGTCTATTTAGGTTTTGTGCTATATATGTATACACCGCTCTTTTTGTGGTTCTATGGTACAACTAAAAATTGTCGAAAGCTTTATGAAGGCAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGCACTTTTGTAATTCGCGGTTCAGAATTTGTTAAACTTACTAATGAGATAGGTGATAGGTTTGAAGCTTATCTCTCAGCGTATGCTAGGCTTAAATACTACTCAGGTACTGGCAGTGAGCAAGATTACTTGCAAGCCTGTCGTGCATGGTTGGCCTATGCACTAGACCAATTTAGAAGCAGTGGTGTAGAAGTAGTTTATACTCCACCACGATATTCAATTGGTGTTAGTAGATTACAAGCTGGTTTTAAGAAACTAGTATCTCCTAGTAGTGCTGTTGAGAAGTGCATTGTTAGTGTATCCTATAGGGGTAATAATCTTAATGGATTGTGGTTGGGTGACTCCATTTACTGTCCACGGCATGTGTTAGGTAAGTTTAGTGGTGACCAATGGGGTGATGTACTTAATCTTGCTAATAATTATGAGTTTGAAGTTGTAACTGGAAATGGTGTTACTTTGAGTGTTGTTAGTAGGCGTTTGAAAGGTGCAGTTTTAATTTTACAAACTGCAATTGTCAATGCTGAAACTCCAAAGTACAAATTTATGAAAGCAAATTGTGGTGATAGTTTCACAATAGCTTGCTCTTATGGAGGTACAGTTATAGGACTTTACCCCGTTACTATGCGTTCTAATGGAACTATTAGAGCATCTTTTCTTGCTGGAGCATGCGGTTCAGTAGGTTTTAATATAGAAAAGGGTGTAGTAAATTTTTACTACATGCACCATCTAGAGTTACCTAATGCATTACATACAGGGACTGACCTAATGGGTGAGTTCTATGGTGGCTATATAGATGAAGAAGTTGCTCAGAAAGTTCAACCCGATAAATTAGTTACTAATAATATTTTAGCATGGCTTTATGCAGCAATTATTAGTGTTAAAGAGAGTAGTTTTTCAACACCTAAGTGGCTTGAAAGTACTACTGTCAGTATTGAAGATTATAATAAGTGGGCAGTGGATAATGGTTTTACATCATTTGTAAGCTGCACTGCTATTACTAAATTAAGTGCTATAACAGGAGTTGATGTTTGTAAACTTCTTCGCACTATTATGGTAAAAAGTACACAATGGGGTAGTGACCCTATTTTGGGACAATATAATTTTGAAGATGAAATGACACCAGAATCTGTTTTTAATCAGGTGGGTGGTGTTAGGTTACAGTCTTCTGTTGTAAAGAGAGCTGTGTCTTGGTTTTGGAGTAGATGTGTGCTAGCTTGCTTTTTATTTGTCTTGTGTTCTATTGTGCTGTTTACAGCCTTACCATATAGATATTATTTATATGGCGCTGCTGTTTTACTTGCAGCTGTGCTCTTTATTTCATTTACTGTGAAACATGTTATGGCATTTATGGATACTTTCCTCTTGCCAACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTACCTTTCATCTACAATACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTAGTCTTTGACACTGTAGTACCGTGGATGTTTCTACCATTAGTCTTGTACACAGCTTTTAAATGTATACAAGGTTGTTATAGTGTAAATTCCTTTAATACTTCTCTGTTAGTGTTGTATCAGTTTATGAAGTTGGGTTTTGTTATATATACCTCTTCTAATACCCTTACAGCCTACTCAGAAGGTAATTGGGAGTTATTCTTTGAGTTAGTGCACACAACTGTGTTGGCTAATGTTAGTAGTAATTCTTTAATTGGTTTAATTGTGTTTAAATTTGCTAAGTGGATGTTGTATTATTGTAATGCCTCATACCTTAATAATTATGTCCTAATGGCTGTTATTGTTAATGGCATAGGTTGGATGTTTACTTGTTACTTTGGATTTTATTGGTGGATTAATAAGGTTTTTGGTTTAACCTTAGGTAAATATAGTTTCAAAGTTTCAGTAGATCAATATAGGTATATGTGTCTTCATAAGATTAACCCACCTAAAACTGTGTGGGAAGTCTTTTCTACAAATATACTTATACAAGGTATAGGTGGTGACCGTGTGTTGCCTATAGCTACAGTGCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTCTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTAGAGTTACACAATAAAATTCTTGCCTCAGATGATGTTAATGAGTGCATGGATAATTTACTGGGTATGCTTGTAACACTATTTTGTGTAGATAGTACTATTGATTTAAGTGAGTACTGTGATGATATACTTAAGAGGTCTACTGTTTTACAGTCAGTCACTCAAGAGTTTTCACACATACCTTCTTATGCAGAGTATGAAAGAGCTAAAGACCTCTATGAAAAGGTTTTAGCTGAGTCTAAAAATGGTAGTGTTGCACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCAATCTTTGATAGAGACTTGGCTGTTCAGAAGAAGTTGGATAGCATGGCTGAACGTGCTATGACAACTATGTATAAGGAAGCGCGTGTCACTGATAGAAGAGCTAAACTTGTTTCGTCATTACATGCGCTACTATTTTCTATGCTTAAGAAGATAGATTCTGAAAAGCTTAATGTACTATTTGACCAGGCAAGTAGTGGTGTCGTACCGCTAGCTACTGTTCCAATAGTTTGTAGTAATAAGCTTACTCTTGTAGTGCCAGATCCAGAGACGTGGATCAAGTGCGTAGAGGGCATGCATGTTACATATTCAACAGTTGTTTGGAATATTGATAATGTTATTGATGCAGATGGCACTGAATTGCAACCAATTTCTACAGGTAATGGTTTAATTTACTGTATAAGTGGTGATAATATAGCATGGCCTCTTAAGGTCAATTTGACTAGGAATGTGCATAATAAAGTTGATGTAGTATTGCAGAACAACGAGCTTATGCCTCATGGTGTTAAAACAAAGGCATGCGTGGCAGGTGTAGATCAAGCACACTGCAGCGTAGAGTCTAAATGTTATTATACTAATATTAGTGGTAATTCAGTAGTGGCTGCTATTACCTCTTCAAATCCTAATTTGAAAGTTGCCTCATTTTTAAATGAAGCAGGCAATCAAATTTATGTTGACTTAGACCCACCATGTAAATTTGGCATGAAGGTGGGTGATAAAGTAGAAGTTGTTTACTTGTATTTTATAAAGAATACAAGGTCAATTGTCAGAGGTATGGTACTTGGTGCTATATCTAATGTTGTGGTTTTACAGTCTAAAGGATATGAGACAGAGGAAGTTGATGCTGTTGGCATACTATCACTTTGTTCTTTTGCAGTAGATCCGGCTGACACGTATATTAAATATGTGGCTGCAGGTAACCAACCTTTAGGTAACTGTGTAAAAATGTTGACAGTTCATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGCCCAACTCCAGACCAGGATTCTTACGGAGGGGCTTCTGTGTGTCTCTATTGCAGGGCACATATAGCTCACCCAGGAAGTGCTGGAAATTTAGATGGACGTTGTCAATTTAAAGGTTCTTTTGTACAAATACCTACTACGGAGAAAGACCCTGTAGGATTCTGTCTACGTAATAAGGTTTGCACTGTTTGTCAGTGTTGGATAGGTCATGGATGTCAATGTGATTCACTTAGACAACCAAAACCTTCTGTTCAATCAGATGCTGGTGCACCAGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGASSQNQRSVILVAKDIPDQLCDALFFYTSHNPKDYADAFAFRQKFDCSLGAGKQFKFETVCGPFFLKGVDKITPGVPAKVIKATSKLADLEDIFGVSPLARKYRELLKTACQWSLTVEALNARAQTLAEIFEPTEIFWLQVAANIQVSAMAMRRLVGEVTAKVMDSLGSSLSALFHVVKETMTRIFQKALAIFESVSELPQRIAALKMAFAKCARSITVVVVGHALTIREFAGTCLASINGAVAKFFEELPTGFMGAKVFSTLAFFKEAAVKIVENIPNAPRGTKGFEIVGNAKGTQVVVRGMRNDLTLLDQKAEVSVEPEGWSAILEGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLSDGVTPEINDGLVLAAIYSSFSVSELVAALKKGEPFKFLGHKFVYAKEAAVSFTLAKAATIGDVLKLFQSARVKTKDVWSALTEQSFEFWKLAYNRVRNLEEVLKTHFCKAQMSIVVLAVVLGEGIWHFCSQVIYKLSGLFSKVVDFCEKHWKGFCTQLRKARLVVTENLCVLKGIAQHCFQLLLEAIHSMYKSFKKCALGRISGDLFFWKGGVHKIIHDGDEIWFDAVDTIEVEDLGTTQEKLIDFEVCEDVILPENQPGHMVQIQDDGKNYMFFRFKKDENIYYTPMSQLGAINVICKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLTVIYDKMCEDLKLFSEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYESDDDIEEEDAEECDTDPADAEECDTASECEEEDEDTKVLSLLQDPASNKYPLPLDDDYSVYNGCIVHKDALDVVNLPSGEETFVVNNCFEGAVKPLPRKVVDVLGDWGEAVDAQEQLCEQESESVSGSAIEDAVVVEQEVVPVVEESQEIVVFTPADLEVAKETSEDVDEFVLVADVPTEEVVPLEKEESQVEQGPIQVVKSQREKKAKKFKVRQTTCEKPKFLEYTTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQKLVTPSYVKGIQCVNNVVGPRHGDKNLEEKLVAAYKSVLVDGVVNYVVPVLSSGIFGVDFKTSIDAMRKAFEGLNIRVLLFSLSQEHIDYFDITCKQKKIYLTEDGVKYRSVVVKPGDSLGQFGQVFVKNKTVFTADDVEDEEVLFIPTTDKAVLEYYGLDAQKYVIYLQTLAQKWEVQYRDNFILLKWRDGNCWISSSIVLLQAAKIRFKGFHAEAWAKLLGGDPTEFVAWCYASCNAKVGDFSDCNWLLANLAEYFDADYTNALLKKRVSCNCGVKSYEFRGLEACIQPVRAPNLLHFKTQYSNCPTCGANSTDEVVEASLPYLLLVATDGPAAVDCDENAAGSVVFIGSTNSGHCYTQAAGKAFDNLAQDRKFGKRSPYITAMYTRFSLKNENSLSIAKMSKSKSKVVKEDVSNLATSSKFSFDDLTDFEQWYDSNIYESLKVQETPENMDEYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLIPDTDENSKAPVYYPTLDSISLKAIWVEGSANFIVGHPNYNSKVLRVPTFWECAESFVKIGEKVDGVTMGLWRAEHLNRPNLERIFNVVKKTIVGTSVVTTQCGKLISKAATFVADKLGDGVVRNVTDRIKGCFGFTRDHFERRVSTQFFKTLFFFLFCLVKASVKGLMASYKSVLCKVLFTALLIIWFVYTSNPVIFTGIHVLDFLFEGSFCSPYADYGKESFDILRYCGSDFTCRVCLHGKDSLHLYKHAYSVEQFYKDAVNGISFTWNWLYMLFLILFVKPVAGFVIICYCIRYLVLSTTVLQTGVGFLDWFIQTVFANFNFMGAGFYFWLFYKIYIQVHHIMYCKDITCEVCKRVALSNRREVSVVVNGRKQLVHVYTNSGYTFCKKHNWYCKNCDKYGHQNTFMSPEVAGELSEKLKRHVKPTAHAYHVVDDACLVDDFVNLKYNAAIPGKEGVHSAVKCFSVSDFLKKAVFLKEAQKCEQISNDSFIVCNTHSAHSLEEAKNAAIYYAQCLCKPILILDQALYEQLTVEPVSKSVVDKVCSILSNIISVDSAALNYKAGTIRDILLSVTKDEEAVDMAIFCHNNDVEYTNDGFTNIVPSYGIDTDKLTPRDRGFLISADAAIANLKVKNSPPVVWKYSDLVKLSDSCLKYLISATVKSGGRFFITRSGAKQVIACYTQKLLVEKKAGGVVSSTISWFKSCFKWLLVFYLLFTACCLGYYQWEMSREFAHPMYDVNSTMHVEGFKVIDKGVLRDIVPEDTCFSNKYVNFDSFWGKPYVNSRDCPIVTAIIDGSGTVAAGVPGFLQWVMDGAMFIHMTQTERRPWYIPTWFNREIVGYTHDSIVTEGEFYTSIALFAARCLYLTSSNTPQLYCFNGDSDAPGALPFANILPHRVYFQPNGVRLIVPQQIMHTPYIVRLVSDSYCRGSVCEYTKPGYCVSLNSQWVLFNDEYTSKPGVFCGSTVRELLFSMVSTFFTGVNPNIYMQLATMFLILVAVVLVFAMVIKFQGVFKAYATTVFTIMLVWLINAFVLCVHSYNSVLAIILLVIYCYVSLVTSRNTAIVMHCWLVFTFGLIVPNWIVCVYLGFVLYMYTPLFLWFYGTTKNCRKLYEGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDRFEAYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQFRSSGVEVVYTPPRYSIGVSRLQAGFKKLVSPSSAVEKCIVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWGDVLNLANNYEFEVVTGNGVTLSVVSRRLKGAVLILQTAIVNAETPKYKFMKANCGDSFTIACSYGGTVIGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLMGEFYGGYIDEEVAQKVQPDKLVTNNILAWLYAAIISVKESSFSTPKWLESTTVSIEDYNKWAVDNGFTSFVSCTAITKLSAITGVDVCKLLRTIMVKSTQWGSDPILGQYNFEDEMTPESVFNQVGGVRLQSSVVKRAVSWFWSRCVLACFLFVLCSIVLFTALPYRYYLYGAAVLLAAVLFISFTVKHVMAFMDTFLLPTLITVIIGVCAEVPFIYNTLISQIVIFFSQWYDPVVFDTVVPWMFLPLVLYTAFKCIQGCYSVNSFNTSLLVLYQFMKLGFVIYTSSNTLTAYSEGNWELFFELVHTTVLANVSSNSLIGLIVFKFAKWMLYYCNASYLNNYVLMAVIVNGIGWMFTCYFGFYWWINKVFGLTLGKYSFKVSVDQYRYMCLHKINPPKTVWEVFSTNILIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVNECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKDLYEKVLAESKNGSVAQQELAAYRKAANIAKSIFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWIKCVEGMHVTYSTVVWNIDNVIDADGTELQPISTGNGLIYCISGDNIAWPLKVNLTRNVHNKVDVVLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGYETEEVDAVGILSLCSFAVDPADTYIKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGHGCQCDSLRQPKPSVQSDAGAPGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 643 Identity=86.31%
Cov(Q)=9.08% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3943
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
414.631259999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00427
outside
1 - 1741
TMhelix
1742 - 1764
inside
1765 - 1839
TMhelix
1840 - 1862
outside
1863 - 1876
TMhelix
1877 - 1899
inside
1900 - 2271
TMhelix
2272 - 2290
outside
2291 - 2548
TMhelix
2549 - 2571
inside
2572 - 2575
TMhelix
2576 - 2598
outside
2599 - 2601
TMhelix
2602 - 2621
inside
2622 - 2633
TMhelix
2634 - 2656
outside
2657 - 2878
TMhelix
2879 - 2896
inside
2897 - 3086
TMhelix
3087 - 3109
outside
3110 - 3113
TMhelix
3114 - 3131
inside
3132 - 3143
TMhelix
3144 - 3166
outside
3167 - 3180
TMhelix
3181 - 3203
inside
3204 - 3215
TMhelix
3216 - 3238
outside
3239 - 3247
TMhelix
3248 - 3270
inside
3271 - 3289
TMhelix
3290 - 3312
outside
3313 - 3943
