Strain
Bat_KY417143
(Region: China; Strain: Bat SARS-like coronavirus isolate Rs4081, complete genome.; Date: 18-Sep-12)
Gene
ORF3a
Description
Annotated in NCBI,
ORF3a
Location
GenBank Accession
Full name
Protein 3
Alternative Name
Accessory protein 3
Sequence
CDS
ATGGATTTGTTTATGAGTATTTTCACGCTTGGATCAATCACACGTCAACCGAGTAAGATTGAAAATGCTTCTCCTGCAAGTACTGTTCATGCTACTGCAACGATACCGCTACAAGCCTCACTCCCTTTTGGATGGCTTGTTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAGTGATTGCGCTTCATAAGAGATGGCAGCTCGCTTTGTATAAAGGCATGCAGCTTGTTTGCAATCTGCTGCTACTCTTTGTGACAATTTATTCACACCTCTTACTTTTAGCTGCTGGCATGGAGGCACAATTTTTGTACATCTATGCTCTGATTTATATTCTGCAAGTTGTGTGCTTCTGCAGATTTATTATGAGATGCTGGCTTTGCTGGAAGTGCAAATCCAAAAACCCATTACTCTATGATGTCAACTACTTTGTTTGCTGGCATACACATAACTATGACTACTGTATACCATACAACAGTGTCACAGATACAATTGTCGTTACTGCAGGTGACGGCATTTCAACACCAAAACTCAAAGAAGACTACCAAATTGGTGGTTATTCTGAGGATTGGCACTCAGGTGTTAAAGACTATGTCGTTGTACATGGCTATTTCACCGAAGTTTACTACCAGCTTGAGTCTACACAAATTACTACAGACACTGGTATTGAAAATGCTACATTCTTCATCTTTAACAAGCTTGTTAAAGATCCACCGAATGTGCAAATACACACAATCGACGGCTCTTCAGGAGTTGTAAATCCAGCAATGGATCCAATTTATGATGAGCCGACGACGACTACTAGCGTGCCTTTGTAA
Protein
MDLFMSIFTLGSITRQPSKIENASPASTVHATATIPLQASLPFGWLVVGVALLAVFQSASKVIALHKRWQLALYKGMQLVCNLLLLFVTIYSHLLLLAAGMEAQFLYIYALIYILQVVCFCRFIMRCWLCWKCKSKNPLLYDVNYFVCWHTHNYDYCIPYNSVTDTIVVTAGDGISTPKLKEDYQIGGYSEDWHSGVKDYVVVHGYFTEVYYQLESTQITTDTGIENATFFIFNKLVKDPPNVQIHTIDGSSGVVNPAMDPIYDEPTTTTSVPL
Summary
Function
Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (By similarity).
Subunit
Homotetramer composed of two homodimers linked non covalently. Interacts with M, S and E proteins. Also interacts with the accessory protein 7a (By similarity).
Miscellaneous
Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).
Keywords
Apoptosis
Glycoprotein
Host cell membrane
Host cytoplasm
Host Golgi apparatus
Host membrane
Ion channel
Ion transport
Membrane
Secreted
Transmembrane
Transmembrane helix
Transport
Viral ion channel
Virion
Feature
chain Protein 3
Uniprot
Pubmed
EMBL
Proteomes
Pfam
PF11289
APA3_viroporin
Interpro
IPR024407
SARS_coronavirus_Orf3/3a
ProteinModelPortal
Ontologies
GO
GO:0044385 C:integral to membrane of host cell
GO:0005216 F:ion channel activity
GO:0020002 C:host cell plasma membrane
GO:0051259 P:protein complex oligomerization
GO:0005576 C:extracellular region
GO:0044178 C:host cell Golgi membrane
GO:0019012 C:virion
GO:0039707 P:pore formation by virus in membrane of host cell
GO:0016021 C:integral component of membrane
GO:0005216 F:ion channel activity
GO:0020002 C:host cell plasma membrane
GO:0051259 P:protein complex oligomerization
GO:0005576 C:extracellular region
GO:0044178 C:host cell Golgi membrane
GO:0019012 C:virion
GO:0039707 P:pore formation by virus in membrane of host cell
GO:0016021 C:integral component of membrane
Subcellular Location
From MSLVP
Host nucleus > host nucleolus. Host cytoplasm. Secreted.
From Uniprot
Virion
The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host Golgi apparatus membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cell membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Secreted The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cytoplasm The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host Golgi apparatus membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cell membrane The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Secreted The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Host cytoplasm The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures (By similarity). With evidence from 1 publications.
Topology
Length:
274
Number of predicted TMHs:
3
Exp number of AAs in TMHs:
64.9297700000001
Exp number, first 60 AAs:
19.7549
Total prob of N-in:
0.00226
POSSIBLE N-term signal
sequence
outside
1 - 43
TMhelix
44 - 66
inside
67 - 78
TMhelix
79 - 101
outside
102 - 104
TMhelix
105 - 124
inside
125 - 274
