Strain
Human_229E_KY674919
(Region: USA; Strain: Human coronavirus 229E strain N08-434B, complete genome.; Date: 2016)
Gene
replicase polyprotein 1a
Description
Annotated in NCBI,
replicase polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCCTGCAACCGTGTGACACTTGCCGTAGCAAGTGATTCTGAAATTTCTGCATATGGCTGTTCTACTATTGCGCAAGCCGTCCGCCGTTATAGCGAGGCCGCTAGCAATGGTTTTAGGGCATGCCGATTTGTTTCATTAGATTTGCAGGATTGCATCGTTGGCATTGCAGATGATACATATGTTATGGGTCTGCATGGCAATCAGACGTTGTTTTGCAACATAATGAAATTTTCTGACCGTCCTTTTATGCTTCATGGGTGGTTGGTTTTTTCCAATTCAAATTACCTTTTGGAGGAATTTGATGTTGTCTTCGGTAAGAGAGGTGGTGGTAATGTGACATACACTGACCAGTATCTCTGTGGCGCCGATGGCAAACCTGTTATGAGTGAAGATTTATGGCAGTTTGTTGACCATTTCGGTGAGAACGAAGAAATTGTCATCAATGGTCGTACTTACGTTTGTGCTTGGCTTACTAAGCGTAAGCCCTTAGATTACAAACGTCAGAACAACCTCGCCATTGAAGAGATTGAATACGTGCATGGTGATGCTTTGCACACACTACGCAATGGTTCTGTTCTTGAAATGGCTAAGGAAGTGAAGACATCTAGTAAAGTTGTGTTAAGCGATGCTCTTGACAAACTTTACAAAGTCTTTGGTTCTCCTGTTATGACAAATGGTTCCAACATCCTAGAGGCCTTTACTAAACCTGTGTTTATTAGTGCATTAGTTCAATGTACTTGTGGTACCAAGTCTTGGTCTGTGGGTGATTGGACCGGTTTTAAATCCTCTTGTTGCAACTTGATCAGTAATAAACTGTGTGTTGTGCCCGGTAATGTTAAACCTGGTGATGCTGTGATTACTACTCAGCAAGCTGGTGCTGGTGTTAAGTATTTTTGTGGCATGACTCTTAAGTTTGTTGCAAATATTGAAGGTGTCTCTGTTTGGAGAGTGATTGCTCTTCAGAGTGTTGATTGCTTTGTTGCTTCTTCCACTTTTGTAGAAGAGGAACATGTTAATAGAATGGACACATTCTGTTTCAATGTACGCAATAGTGTTACTGATGAGTGTCGTCTGGCCATGTTGGGTGCTGAAATGACTAGTAATGTCAGAAGACAAGTTGCTTCAGGTGTCATAGACATTAGTACCGGCTGGTTTGATGTTTATGATGACATCTTTGCTGAAAGCAAACCATGGTTTGTTCGCAAGGCTGAAGACATTTTTGGCCCTTGTTGGTCCGCTCTTGTTTCTGCACTTAAACAACTTAAAGTCACTACAGGTGAACTTGTGAGATTTGTTAAGTCTATTTGCAATTCAGCTGTTGCTGTTGTGGGTGGTACTATACAAATTTTCGCTAGTGTGCCTGAGAAGTTTTTGAATGCGTTTGACGTGTTTGTCACAACTATTCAAACTGTCTTTGACTGTGCTGTTGAAACTTGTACTATTGCCGGTAAAGCATTTGACAAGGTTTTTGACTATGTCTTGCTTGATAATGCGCTTGTAAAACTTGTCACCACAAAGCTTAAGGGTGTTCGTGAAGGTGGCCTTAATAAAGTTAAGTATGCAACAGTTGTTGTTGGTTCCACTGAAGAAGTTAAATCTTCACGTGTTGAACGTAGCAATGCTGTACTTACAATCGCCAACAATTATTCCAAACTTTCTGATGAAGGGTATACTGTTGTAATTGGCGATGTGGCGTACTTTGCTAGTGACGGCTACTTCCGTCTTATGGCCAGTCCAAATAGTGTGTTGACTACTGCAGTCTATAAACCATTGGTTGATTTTAATGTGAATGTTATTGGTACTAGACCTGAAAAATTTCCAACCACTGTGACTTGTGAAAATTTAGAGTCTGCTGTTTTGTTTGTTAATGACAAAATCACTGAATTCCAATTGGATTACAGTATTGATGTCATTGATAATGAAATAATTGTCAAACCTAATATCAGCTTATGTGTTCCACTTTATGTGAGAGACTATGTTGACAAATGGGATGATTTTTGCAGACAATATAGTAACGAGTCTTGGTTTGAGGATGATTACAAGGCTTTTATCAGTGTTTTGGATATCACTGATGCTGCTGTGAAAGCTGCAGAGTCTAAAGCCTTCGTTGATACTATTGTTCCACCTTGCCCATCTATTTTGAAAGTTATAGATGGAGGCAAAATATGGAATGGTGTTATTAAAAATGTTAACTATGTTAGAGACTGGCTTAAGTCTTTGAAGTTAAATCTCACACAACAGGGTTTGCTTGGAACATGTGCAAAGCGTTTTAAACGTTGGCTTGGCATTTTGCTAGAGGCCTATAATGCGTTTTTAAACACTGTTGTTTCTACTGTTAAAATTGGTGGTTTGACCTTTAAAACATATGCTTTTGATAAACCTTACATTGTGATACGTGATATCGTGTGTAAGGTTGAAAATAAAACAGAAGCAGAATGGATTGAACTTTTTCCACATAATGACAGGATTAAGTCCTTTAGTACTTTTGAGAGTGCTTACATGCCAATTGCAGACCCTACACATTTTGACATTGAAGAAGTTGAACTTTTAGATGCAGAGTTTGTAGAACCAGGCTGTGGTGGTATTTTGTCAGTAATAGATGAGCACGTCTTTTATAAGAAGGATGGTGTTTATTATCCATCAAATGGTACTAACATTCTACCTGTTGCATTTACAAAAGCCGCTGGTGGTAAAGTTTCATTTTCTGATGACGTTGAAGTAAAAGACATTGAACCTGTTTACAGAGTCAAGCTTTGCTTTGAGTTTGAAGATGAAAAACTTGTAGATGTTTGTGAAAAGGCAATTGGCAAGAAAATTAAACATGAAGGTGACTGGGATAGCTTTTGTAAGACTATTCAATCAGCACTTTCTGTTGTTTCATGCTATGTAAATCTACCTACTTATTACATTTATGATGAAGAAGGCGGTAATGACTTGAGTTTGCCCGTTATGATTTCTGAATGGCCTCTTTCTGTTCAACAAGCTCAACAAGAAGCTACTCATATTGCTGAGGATGTTGTTGACCAAGTTGAAGAAGTCAATAGCATTTTTGACATTGAGACAGTGGATGTTAAGCATGAGATGAGTCCTTTTGAAATGCCATTTGAAGAGTTAAATGGTTTAAAGATACTTAAACAATTGGATAACAACTGCTGGGTTAACTCAGTTATGTTACAAATACAATTAACTGGTATACTCGATGGTGATTATGCTATGCAGTTTTTTAAAATGGGCCGAGTTGCCAAGATGATTGAACGCTGCTACACTGCTGAGCAATGTATACGTGGTGCTATAGGTGATGTTGGTTTGTGTATGTATAGACTTCTTAAAGACTTACACACTGGTTTTATGGTTATGGATTATAAATGTAGTTGTACCAGTGGTAGGCTTGAAGAATCGGGAGCTGTTTTGTTTTGTACGCCCACTAAGAAGGCGTTTCCTTATGGTACTTGTCTAAATTGTAACGCACCTCGCATGTGTACAATTAGGCAGTTACAAGGTACCATAATATTTGTGCAACAAAAACCAGAACCTGTTAACCCTGTTTCTTTTGTTGTTAAACCAGTCTGCTCATCAATTTTTCGTGGTGCTGTGTCTTGTGGTCATTACCAGACTAACATCTATTCACAAAATTTGTGTGTGGATGGTTTTGGTGTTAACAAGATTCAGCCCTGGACAAATGATGCACTTAATACTATTTGTATTAAGGATGCAGATTATAATGCAAAAGATGAAATATCTGTTACACCAATTAAAAACACAGTTGATACAATACCTAAGGAAGAATTTGTTGTTAAAGAGAAGTTGAACGCCTTTCTCGTTCATGGCAATGTAGCTTTCTACCAAGGTGATGTTGATACTGTTGTTAATGGTGTTGACTTTGACTTTATTGTAAATGCTGCTAATGAGAACCTTGCTCATGGTGGAGGACTTGCCAAAGCTTTAGATGTGTACACTAAAGGTAAACTTCAACGTTTATCCAAAGAACACATTGGATTAGCGGGTAAAGTAAGAGTTGGTACAGGAGTTATGGTTGAGTGTGATAGCCTTAGAATTTTTAATGTTGTTGGTCCACGCAAGGGTAAACATGAACGTGATTTACTCGTAAAAGCTTACAACACTATTAATAATGAACAAGGCACACCTTTAACACCAATTTTGAGTTGTGGTATTTTTGGTGTCAAACTCGAAACTTCATTGGAAGTTTTGCTTGATGTTTGTAATACAAAAGAATTTAAAGTTTTTGTTTATACAGACACAGAGGTTTGTAAGGTTAAGGATTTTGTGTCTGGTTTAGTGAATGTTCAAAAAGTTGCACAACCTAAAATAGAACAAAAACCAGTGTCCTTAATTAAAGTTGCACCCAAGCCTTATAGGGTAGATGGTAAATTTAGTTACTTTACAGAAGACTTGTTGTGTGTCGCTGATGACAAACCCATTGTTTTGTTTACTGACTCTATGCTTACTTTGGATGACCGTGGTTTGGCTCTAGACAATGCACTTAGTGGTGTGCTTAGTGCTGCTATTAAGGATTGTGTTGACATAAATAAAGCTATACCTTCTGGTAATCTTATTAAGTTTGATATAGATTCTGTTGTTGTTTACATGTGTGTTGTGCCATCCGAAAAGGACAAACATTTAGATAATAATGTTCAACGATGCACACGTAAGTTGAATAGACTTATGTGTGATATAGTTTGTAATATACCAGCTGACTACATCTTGCCATTGGTGTTGTCTAGTTTGACTTGTAATGTTTCTTTTGTAGGTGAACTTAAAGCTGCTGAAGCTAAAGTTATAACTATAAAGGTGACAGAGGATGGTGTTAATGTTCATGATGTGACCGTGACAACAGATAAGTCATTTGAACAACAAGTTGGTGTTATTGCTGACAAGGACAAAGATCTTTCTGGTGCAGTACCCAGTGATCTTAACACATCTGAATTGCTTACTAAAGCAATAGATGTTGATTGGGTCGAATTTTATGGCTTTAAAGATGCTGTTACTTTTGCAACAGTTGATCATAGTGCTTTTGCCTATGAAAGTGCTGTTGTTAATGGTATTAGAGTGTTAAAAACCAGTGATAATAATTGTTGGGTGAATGCTGTTTGTATTGCACTACAGTATTCGAAACCTCATTTTATTTCACAAGGTCTTGATGCTGCGTGGAATAAATTTGTTTTAGGCGATGTTGAAATTTTTGTTGCATTTGTTTACTATGTTGCAAGACTAGTGAAAGGTGACAAGGGTGATGCTGAAGACACTTTGAATAAGTTGTCTAAGTATCTTGCTAATGAAGCTCAAGTTCAATTAGAACATTATAGTTCTTGTGTTGAATGTGAAGCTAAATTTAAAAACTCTGTTACATCTATCAATTCTGCTATAGTTTGTGCTAGTGTCAAACGTGATGGTGTGCAAGTTGGTTATTGTGTCCATGGTATTAAGTACTATTCACGTGTTAAAAGTGTTAGAGGTAGAGCTATTATAGTCAGTGTCGAACAGCTTGAACCGTGTGTTCAGTCTAGACTTTTGAGTGGTGTTGCTTATACTGCTTTTTCTGGACCTGTTGATAAAGGTCATTATACTGTTTATGACACTGCAAAGAAATCAATGTATGATGGTGATCGTTTTGTTAAACATGATCTTTCTCTGCTGTCTGTCACATCAGTTGTTATGGTTGGTGATTATGTTGCACCTGTTAGTACAGTGAAACCTAAACCAGTCATTTCTCAACTTGATGAAAATGCACAGAAGTTCTTTGATTTTGGTGATTTTTTGGTTCATAATTTTGTTATTTTTTTCACATGGTTATTGAGTATGTTTACTTTGTGTAAAACTGCAGTAACTACATGTGATGTTAAAATAATGGCCAAAGCACCACAAAGGACGGGTGTTGTTTTAAAACGTAGTCTTAAATATAACTTAAAAGCGTCAGCAGCTGTTCTTAAATCTAAGTGGTGGCTGCTTGCTAAGTTTACGAAACTACTGTTACTTATATATACATTGTATTCAGTAGTTTTGTTTTGTGTACGTTTTGGACCGTTTAAGTTTTGTAGTGAGACTGTTAATGGTTATGCTAAGTCAAACTTTGTCAAGGATGATTACTGTGATGGTTCATTGGGCTGCAAGATGTGTCTTTTTGGTTATCAAGAGTTAAGTCAATTTAGCCATTTGGATGTTGTGTGGAAGCATATAACAGACCCTTTGTTTAGTAATATACAACCTTTCATTGTCATGGTTTTGCTGCTTATATTTGGTGACAATTATTTGAGATGCTTCTTGCTGTATTTTGTTGCTCAGATGATAAGCACAGTTGGTGTTTTTCTAGGTTATAAGGAAACAAATTGGTTCTTGCACTTTATTCCATTTGATGTTATTTGTGATGAACTGTTTGTCACTGTTATTGTTATTAAGGTTATTTCTTTTGTCAGACATGTGCTTTTTGGTTGTGAAAACCCAGATTGCATTGCGTGTTCTAAGAGTGCTAGACTTAAGAGATTCCCTGTTAACACAATTGTTAATGGTGTGCAACGTTCATTTTATGTTAATGCAAATGGTGGTAGTAAGTTTTGTAAGAAACATAGATTTTTCTGTGTTGATTGTGACTCTTATGGTTATGGCAGCACGTTTATAACACCCGAAGTTTCTAGAGAACTTGGTAACATTACTAAAACAAATGTGCAACCAACAGGTCCAGCGTATGTCATGATTGACAAAGTGGAGTTTGAAAATGGTTTTTACAGGTTGTATTCCGGTGAAACATTTTGGCGTTACAACTTTGATATAACTGAAAGCAAGTATTCTTGCAAAGAGGTTTTTAAAAATTGTAATGTTTTGGATGATTTCATCGTGTTTAACAATAATGGGACCAATGTAACGCAGGTTAAAAATGCTAGTGTTTACTTTTCACAGTTGTTGTGTAGGCCCATTAAATTAGTTGACAGTGAACTTTTGTCCACTTTGTCAGTTGATTTTAATGGTGTCTTACACAAGGCATACATCGATGTACTACGTAATAGCTTTGGTAAAGATCTTAATGCTAATATGTCTTTAGCCGATTGCAAGAGTGCTTTAGGCCTGTCTATTAGTGATCATGAATTTACTAGTGCTATTTCTAATGCACATCGTTGTGACGTGTTGTTATCTGATTTGTCATTTAACAACTTTGTCAGTTCGTATGCTAAACCTGAGGAAAAATTATCAGCTTATGACTTGGCGTGTTGTATGCGTGCAGGTGCTAAGGTTGTTAATGCCAATGTTCTGACAAAGGACCAAACTCCTATTGTTTGGCATGCAAAGGATTTTAACAGTCTTTCTGCTGAAGGTCGTAAGTATATTGTAAAAACTAGCAAAGCTAAGGGTTTGACTTTCTTGTTGACAATTAATGAAAACCAAGCTGTCACGCAAATACCTGCAACTAGCATTGTTGCTAAGCAAGGTGCTGGTGATGCTGGTCATTCATTAACATGGCTGTGGCTACTGTGTGGTCTTGTGTGTTTGATTCAATTCTATTTGTGCTTTTTCATGCCCTATTTTATGCACGATGTCGTGAGTAGTTTTGAGGGTTATGATTTTAAGTATATAGAAAATGGTCAGTTGAAGAATTTTGAAGCGCCACTTAAATGTGTCAGAAACGTTTTTGAAAACTTTGAGGACTGGCATTATGCTAAGTTTGGCTTCACACCTTTAAATAAGCAAAGCTGTCCTATTGTAGTTGGAGTTTCTGAAATTGTTAATACTGTCGCTGGCATTCCATCTAATGTGTATCTTGTTGGTAAAACTTTAGTTTTTACACTACAAGCTGCTTTTGGTAATGCTGGTGTTTGTTATGACATTTTTGGAGTCACAACACCTGAAAAGTGCATTTTTACTTCTGCTTGTACTAGATTAGAAGGTTTGGGTGGTAACAATGTTTATTGTTATAACACAGAGCTTATGGAAGGTTCTCTGCCGTACAGTTCAATACAAGCTAATGCATATTATAAATATGATAATGGCAATTTTATTAAGTTGCCAGAAGTTATTGCACAAGGCTTTGGCTTTAGAACAGTACGTACTATTGCTACCAAATACTGCCGCGTAGGTGAATGTGTTGACTCCAATGCAGGTGTGTGTTTTGGCTTTGACAAGTGGTTTGTTAATGATGGACGTGTTGCCAATGGTTATGTTTGTGGTACTGGTTTGTGGAACCTTGTATTTAACATACTTTCCATGTTCTCATCTTCATTCTCTGTTGCTGCAATGTCAGGTCAAATTTTACTTAATTGTGCATTAGGTGCTTTTGCTATCTTTTGTTGTTTTCTTGTGACAAAGTTTAGACGCATGTTTGGTGACCTTTCTGTAGGTGTTTGTACTGTTGTTGTGGCTGTTTTGCTTAACAATGTCTCTTACATTGTAACTCAGAATCTAGTAACAATGATTGCTTATGCCATATTGTATTTCTTTGCTACTAGAAGCTTACGCTATGCATGGATTTGGTGTGCTGCATATTTAATTGCGTATATTTCTTTTGCTCCATGGTGGTTGTGTGCTTGGTACTTTCTTGCTATGTTGACAGGTTTGTTACCTAGTTTGCTGAAGCTTAAAGTTTCGACAAATCTTTTCGAAGGTGACAAATTTGTAGGTACATTTGAAAGTGCTGCTGCAGGAACATTTGTCATTGACATTCGTTCTTATGAGAAACTTGCTAATAGCATCTCTCCAGAAAAGTTGAAAAGTTATGCTGCTAGTTATAATAGATACAAGTACTATAGTGGTAATGCAAATGAAGCTGATTACCGTTGTGCTTGTTATGCCTATTTAGCAAAAGCAATGTTGGACTTTTCGCGTGATCATAATGACATCTTGTACACACCTCCGACTGTCAGTTATGGTTCTACATTACAGGCTGGTTTGCGCAAAATGGCACAACCATCTGGCTTTGTGGAGAAATGTGTTGTCCGTGTCTGCTATGGAAACACTGTGTTGAATGGGTTGTGGCTTGGTGATATTGTTTATTGCCCACGTCATGTTATCGCATCTAACACAACTTCTGCTATAGATTATGATCACGAATATAGTATTATGCGGTTGCATAATTTCTCTATAATATCTGGTACAGCATTTCTTGGTGTTGTAGGTGCTACTATGCATGGAGTAACTCTTAAAATTAAGGTTTCACAGACTAACATGCACACACCTAGACACTCTTTTAGAACACTAAAATCTGGTGAAGGTTTTAACATCTTAGCATGCTATGATGGTTGTGCTCAGGGTGTTTTTGGTGTGAACATGAGAACTAATTGGACTATCCGTGGTTCATTTATTAATGGTGCGTGTGGTTCTCCTGGCTACAATCTTAAAAATGGCGAGGTGGAATTTGTTTATATGCATCAAATTGAACTCGGAAGTGGTAGTCATGTAGGTTCTAGCTTTGATGGTGTTATGTATGGTGGTTTTGAAGACCAACCTAATCTTCAAGTTGAATCTGCAAACCAGATGTTAACAGTTAATGTGGTTGCATTTCTTTATGCTGCTATATTGAATGGTTGCACATGGTGGCTTAAAGGTGATAAATTGTTTGTGGAGCATTATAATGAGTGGGCACAGGCTAATGGTTTCACAGCTATGAATGGTGAAGATGCTTTTTCCATTCTTGCTGCTAAAACTGGTGTCTGTGTGGAAAGGTTACTTCATGCTATTCAAGTTTTGAATAATGGATTTGGTGGTAAACAAATTTTGGGTTATTCTAGTCTCAATGATGAGTTCAGTATTAATGAAGTTGTCAAACAAATGTTTGGTGTTAACCTGCAAAGTGGTAAAACCACTAGTATGTTTAAATCCATAAGCTTGTTTGCTGGTTTCTTTGTCATGTTCTGGGCTGAATTATTTGTTTATACCACCACTATTTGGGTTAACCCTGGTTTTCTTACTCCTTTTATGATTTTGCTTGTTGCTTTGTCACTCTGTCTTACATTTGTTGTTAAACATAAGGTTTTGTTTTTGCAAGTGTTTTTGTTGCCTTCAATTATTGTGGCTGCTATTCAAAACTGTGCTTGGGACTACCATATTACAAAGGTGTTGGCAGAGAAGTTTGATTATAATGTTTCTGTTATGCAAATGGACATCCAGGGTTTTGTTAACATTTTTATTTGTCTTTTTGTTGCACTGTTGCATACTTGGCGCTTTGCTAAAGAGCGTTGTACACATTGGTGCACTTATTTGTTCTCACTCATTGCTGTTTTATACACTGCATTGTATAGTTATGACTACGTTAGTTTGCTGGTTATGCTACTTTGTGCAATTTCTAATGAATGGTATATTGGTGCTATCATTTTTAGAATTTGTCGTTTTGGTGTTGCATTTTTACCAGTGGAATACGTGTCTTACTTTGATGGTGTTAAAACTGTGCTGTTGTTTTACATGTTGTTAGGCTTTGTTAGCTGTATGTACTATGGTTTGTTGTACTGGATTAACAGGTTCTGTAAGTGCACATTAGGTGTTTATGATTTCTGTGTTAGTCCAGCCGAATTTAAGTATATGGTTGCTAATGGTTTGAATGCACCAAATGGCCCTTTTGATGCACTCTTTCTGTCTTTTAAACTAATGGGTATTGGTGGTCCTAGAACCATTAAAGTCTCTACTGTACAGTCTAAATTGACTGATCTTAAGTGCACAAACGTCGTTCTAATGGGCATTTTGTCTAACATGAACATAGCTTCTAATTCAAAGGAGTGGGCATATTGTGTTGAAATGCACAATAAAATAAATTTGTGTGACGACCCTGAAACTGCTCAAGAGTTATTGCTGGCGTTGTTGGCCTTTTTCTTGTCTAAGCATAGTGATTTTGGTCTTGGTGATCTTGTCGATTCTTATTTTGAGAACGACTCCATTTTGCAAAGTGTTGCATCTTCTTTTGTTGGTATGCCATCTTTTGTTGCATATGAAACAGCAAGACAAGAGTATGAAAATGCTGTTGCAAATGGTTCCTCACCACAAATAATCAAACAATTGAAGAAGGCTATGAATGTTGCAAAAGCTGAGTTTGACAGGGAGTCATCTGTTCAAAAGAAAATTAACAGAATGGCTGAACAAGCTGCTGCAGCTATGTACAAAGAAGCACGTGCTGTTAATAGAAAATCAAAAGTTGTTAGTGCAATGCATAGTTTACTCTTTGGCATGCTCCGACGTTTGGACATGTCTAGTGTTGACACTATCCTTAATATGGCACGTAATGGTATTGTCCCTCTTTCCGTTATCCCTGCTACTTCTGCAGCCAGGCTCGTCGTCGTAGTGCCAGATCATGATTCATTTGTGAAAATGATGGTAGATGGTTTTGTGCACTACGCTGGTGTTGTTTGGACATTACAGGAAGTTAAGGATAATGACGGTAAGAATGTGCATCTTAAAGATGTTACAAAGGAAAACCAGGAAATACTTGTTTGGCCTCTGATTTTGACTTGTGAACGTGTCGTTAAATTGCAGAACAATGAAATAATGCCAGGCAAGATGAAGGTCAAGGCCACCAAAGGTGAAGGTGATGGAGGCATTATTAGTGAAGGTAATGCTCTATACAACAATGAAGGTGGACGTGCATTTATGTATGCATATGTGACTACGAAGCCTGGCATGAAGTATGTTAAATGGGAACATGACTCTGGTGTGGTTACAGTTGAATTGGAACCACCTTGCAGATTTGTTATAGACACACCTACTGGACCCCAAATTAAGTATCTTTATTTTGTTAAGAATCTTAACAATTTAAGGAGAGGTGCTGTTTTGGGTTACATTGGTGCCACTGTGAGATTGCAAGCTGGCAAACAGACTGAGTTTGTTTCAAACTCCCATTTATTAACACATTGTGCTTTTGCTGTTGACCCAGCTGCAGCCTACCTTGATGCTGTTAAACAAGGCGCAAAACCTGTTGGCAATTGTGTAAAGATGTTGACTAATGGTTCTGGTAGTGGTCAGGCTATTACTAGTACCATTGATTCTAACACTACGCAGGACACATATGGTGGCGCGTCTGTTTGTATTTATTGCAGAGCACATGTTGCACATCCAACCATGGATGGTTTTTGTCAGTACAAAGGTAAGTGGGTACAAGTGCCTATAGGTACAAATGACCCTATAAGATTTTGTCTTGAAAATACTGTTTGTAAAGTTTGTGGTTGTTGGCTTAATCATGGCTGTACATGTGACCGGACTGCTATCCAAAGTTTTGATAGCAGTTATTTAAACGAGTCAGGGGCTCTAGTGCCGCTCGACTAG
Protein
MACNRVTLAVASDSEISAYGCSTIAQAVRRYSEAASNGFRACRFVSLDLQDCIVGIADDTYVMGLHGNQTLFCNIMKFSDRPFMLHGWLVFSNSNYLLEEFDVVFGKRGGGNVTYTDQYLCGADGKPVMSEDLWQFVDHFGENEEIVINGRTYVCAWLTKRKPLDYKRQNNLAIEEIEYVHGDALHTLRNGSVLEMAKEVKTSSKVVLSDALDKLYKVFGSPVMTNGSNILEAFTKPVFISALVQCTCGTKSWSVGDWTGFKSSCCNLISNKLCVVPGNVKPGDAVITTQQAGAGVKYFCGMTLKFVANIEGVSVWRVIALQSVDCFVASSTFVEEEHVNRMDTFCFNVRNSVTDECRLAMLGAEMTSNVRRQVASGVIDISTGWFDVYDDIFAESKPWFVRKAEDIFGPCWSALVSALKQLKVTTGELVRFVKSICNSAVAVVGGTIQIFASVPEKFLNAFDVFVTTIQTVFDCAVETCTIAGKAFDKVFDYVLLDNALVKLVTTKLKGVREGGLNKVKYATVVVGSTEEVKSSRVERSNAVLTIANNYSKLSDEGYTVVIGDVAYFASDGYFRLMASPNSVLTTAVYKPLVDFNVNVIGTRPEKFPTTVTCENLESAVLFVNDKITEFQLDYSIDVIDNEIIVKPNISLCVPLYVRDYVDKWDDFCRQYSNESWFEDDYKAFISVLDITDAAVKAAESKAFVDTIVPPCPSILKVIDGGKIWNGVIKNVNYVRDWLKSLKLNLTQQGLLGTCAKRFKRWLGILLEAYNAFLNTVVSTVKIGGLTFKTYAFDKPYIVIRDIVCKVENKTEAEWIELFPHNDRIKSFSTFESAYMPIADPTHFDIEEVELLDAEFVEPGCGGILSVIDEHVFYKKDGVYYPSNGTNILPVAFTKAAGGKVSFSDDVEVKDIEPVYRVKLCFEFEDEKLVDVCEKAIGKKIKHEGDWDSFCKTIQSALSVVSCYVNLPTYYIYDEEGGNDLSLPVMISEWPLSVQQAQQEATHIAEDVVDQVEEVNSIFDIETVDVKHEMSPFEMPFEELNGLKILKQLDNNCWVNSVMLQIQLTGILDGDYAMQFFKMGRVAKMIERCYTAEQCIRGAIGDVGLCMYRLLKDLHTGFMVMDYKCSCTSGRLEESGAVLFCTPTKKAFPYGTCLNCNAPRMCTIRQLQGTIIFVQQKPEPVNPVSFVVKPVCSSIFRGAVSCGHYQTNIYSQNLCVDGFGVNKIQPWTNDALNTICIKDADYNAKDEISVTPIKNTVDTIPKEEFVVKEKLNAFLVHGNVAFYQGDVDTVVNGVDFDFIVNAANENLAHGGGLAKALDVYTKGKLQRLSKEHIGLAGKVRVGTGVMVECDSLRIFNVVGPRKGKHERDLLVKAYNTINNEQGTPLTPILSCGIFGVKLETSLEVLLDVCNTKEFKVFVYTDTEVCKVKDFVSGLVNVQKVAQPKIEQKPVSLIKVAPKPYRVDGKFSYFTEDLLCVADDKPIVLFTDSMLTLDDRGLALDNALSGVLSAAIKDCVDINKAIPSGNLIKFDIDSVVVYMCVVPSEKDKHLDNNVQRCTRKLNRLMCDIVCNIPADYILPLVLSSLTCNVSFVGELKAAEAKVITIKVTEDGVNVHDVTVTTDKSFEQQVGVIADKDKDLSGAVPSDLNTSELLTKAIDVDWVEFYGFKDAVTFATVDHSAFAYESAVVNGIRVLKTSDNNCWVNAVCIALQYSKPHFISQGLDAAWNKFVLGDVEIFVAFVYYVARLVKGDKGDAEDTLNKLSKYLANEAQVQLEHYSSCVECEAKFKNSVTSINSAIVCASVKRDGVQVGYCVHGIKYYSRVKSVRGRAIIVSVEQLEPCVQSRLLSGVAYTAFSGPVDKGHYTVYDTAKKSMYDGDRFVKHDLSLLSVTSVVMVGDYVAPVSTVKPKPVISQLDENAQKFFDFGDFLVHNFVIFFTWLLSMFTLCKTAVTTCDVKIMAKAPQRTGVVLKRSLKYNLKASAAVLKSKWWLLAKFTKLLLLIYTLYSVVLFCVRFGPFKFCSETVNGYAKSNFVKDDYCDGSLGCKMCLFGYQELSQFSHLDVVWKHITDPLFSNIQPFIVMVLLLIFGDNYLRCFLLYFVAQMISTVGVFLGYKETNWFLHFIPFDVICDELFVTVIVIKVISFVRHVLFGCENPDCIACSKSARLKRFPVNTIVNGVQRSFYVNANGGSKFCKKHRFFCVDCDSYGYGSTFITPEVSRELGNITKTNVQPTGPAYVMIDKVEFENGFYRLYSGETFWRYNFDITESKYSCKEVFKNCNVLDDFIVFNNNGTNVTQVKNASVYFSQLLCRPIKLVDSELLSTLSVDFNGVLHKAYIDVLRNSFGKDLNANMSLADCKSALGLSISDHEFTSAISNAHRCDVLLSDLSFNNFVSSYAKPEEKLSAYDLACCMRAGAKVVNANVLTKDQTPIVWHAKDFNSLSAEGRKYIVKTSKAKGLTFLLTINENQAVTQIPATSIVAKQGAGDAGHSLTWLWLLCGLVCLIQFYLCFFMPYFMHDVVSSFEGYDFKYIENGQLKNFEAPLKCVRNVFENFEDWHYAKFGFTPLNKQSCPIVVGVSEIVNTVAGIPSNVYLVGKTLVFTLQAAFGNAGVCYDIFGVTTPEKCIFTSACTRLEGLGGNNVYCYNTELMEGSLPYSSIQANAYYKYDNGNFIKLPEVIAQGFGFRTVRTIATKYCRVGECVDSNAGVCFGFDKWFVNDGRVANGYVCGTGLWNLVFNILSMFSSSFSVAAMSGQILLNCALGAFAIFCCFLVTKFRRMFGDLSVGVCTVVVAVLLNNVSYIVTQNLVTMIAYAILYFFATRSLRYAWIWCAAYLIAYISFAPWWLCAWYFLAMLTGLLPSLLKLKVSTNLFEGDKFVGTFESAAAGTFVIDIRSYEKLANSISPEKLKSYAASYNRYKYYSGNANEADYRCACYAYLAKAMLDFSRDHNDILYTPPTVSYGSTLQAGLRKMAQPSGFVEKCVVRVCYGNTVLNGLWLGDIVYCPRHVIASNTTSAIDYDHEYSIMRLHNFSIISGTAFLGVVGATMHGVTLKIKVSQTNMHTPRHSFRTLKSGEGFNILACYDGCAQGVFGVNMRTNWTIRGSFINGACGSPGYNLKNGEVEFVYMHQIELGSGSHVGSSFDGVMYGGFEDQPNLQVESANQMLTVNVVAFLYAAILNGCTWWLKGDKLFVEHYNEWAQANGFTAMNGEDAFSILAAKTGVCVERLLHAIQVLNNGFGGKQILGYSSLNDEFSINEVVKQMFGVNLQSGKTTSMFKSISLFAGFFVMFWAELFVYTTTIWVNPGFLTPFMILLVALSLCLTFVVKHKVLFLQVFLLPSIIVAAIQNCAWDYHITKVLAEKFDYNVSVMQMDIQGFVNIFICLFVALLHTWRFAKERCTHWCTYLFSLIAVLYTALYSYDYVSLLVMLLCAISNEWYIGAIIFRICRFGVAFLPVEYVSYFDGVKTVLLFYMLLGFVSCMYYGLLYWINRFCKCTLGVYDFCVSPAEFKYMVANGLNAPNGPFDALFLSFKLMGIGGPRTIKVSTVQSKLTDLKCTNVVLMGILSNMNIASNSKEWAYCVEMHNKINLCDDPETAQELLLALLAFFLSKHSDFGLGDLVDSYFENDSILQSVASSFVGMPSFVAYETARQEYENAVANGSSPQIIKQLKKAMNVAKAEFDRESSVQKKINRMAEQAAAAMYKEARAVNRKSKVVSAMHSLLFGMLRRLDMSSVDTILNMARNGIVPLSVIPATSAARLVVVVPDHDSFVKMMVDGFVHYAGVVWTLQEVKDNDGKNVHLKDVTKENQEILVWPLILTCERVVKLQNNEIMPGKMKVKATKGEGDGGIISEGNALYNNEGGRAFMYAYVTTKPGMKYVKWEHDSGVVTVELEPPCRFVIDTPTGPQIKYLYFVKNLNNLRRGAVLGYIGATVRLQAGKQTEFVSNSHLLTHCAFAVDPAAAYLDAVKQGAKPVGNCVKMLTNGSGSGQAITSTIDSNTTQDTYGGASVCIYCRAHVAHPTMDGFCQYKGKWVQVPIGTNDPIRFCLENTVCKVCGCWLNHGCTCDRTAIQSFDSSYLNESGALVPLD
Summary
Function
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Catalytic Activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
Proteomes
PRIDE
Pfam
Interpro
IPR037230
NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR014829 NSP8
IPR011050 Pectin_lyase_fold/virulence
IPR036499 NSP9_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR023298 ATPase_P-typ_TM_dom_sf
IPR032505 Corona_NSP4_C
IPR036333 NSP10_sf
IPR014828 NSP7
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR014829 NSP8
IPR011050 Pectin_lyase_fold/virulence
IPR036499 NSP9_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR023298 ATPase_P-typ_TM_dom_sf
IPR032505 Corona_NSP4_C
IPR036333 NSP10_sf
IPR014828 NSP7
SUPFAM
ProteinModelPortal
PDB
2ZU2
E-value=0.0 Score= 632 Identity=99.67%
Cov(Q)=7.40% Cov(P)=100.00%
3D Structure
Ontologies
GO
GO:0039520 P:induction by virus of host autophagy
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0004197 F:cysteine-type endopeptidase activity
GO:0019079 P:viral genome replication
GO:0033644 C:host cell membrane
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0016021 C:integral component of membrane
GO:0008242 F:omega peptidase activity
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0004197 F:cysteine-type endopeptidase activity
GO:0019079 P:viral genome replication
GO:0033644 C:host cell membrane
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0016021 C:integral component of membrane
GO:0008242 F:omega peptidase activity
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0039548 P:suppression by virus of host IRF3 activity
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 3 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 3 publications.
Topology
Length:
4083
Number of predicted TMHs:
20
Exp number of AAs in TMHs:
428.043869999999
Exp number, first 60 AAs:
0.0061
Total prob of N-in:
0.00431
outside
1 - 1926
TMhelix
1927 - 1949
inside
1950 - 1986
TMhelix
1987 - 2009
outside
2010 - 2064
TMhelix
2065 - 2087
inside
2088 - 2093
TMhelix
2094 - 2111
outside
2112 - 2120
TMhelix
2121 - 2143
inside
2144 - 2490
TMhelix
2491 - 2513
outside
2514 - 2569
TMhelix
2570 - 2592
inside
2593 - 2718
TMhelix
2719 - 2738
outside
2739 - 2747
TMhelix
2748 - 2770
inside
2771 - 2776
TMhelix
2777 - 2794
outside
2795 - 2797
TMhelix
2798 - 2817
inside
2818 - 2823
TMhelix
2824 - 2846
outside
2847 - 3029
TMhelix
3030 - 3049
inside
3050 - 3275
TMhelix
3276 - 3298
outside
3299 - 3302
TMhelix
3303 - 3322
inside
3323 - 3328
TMhelix
3329 - 3351
outside
3352 - 3365
TMhelix
3366 - 3388
inside
3389 - 3400
TMhelix
3401 - 3423
outside
3424 - 3432
TMhelix
3433 - 3452
inside
3453 - 3463
TMhelix
3464 - 3486
outside
3487 - 4083
