Strain
Murine_MH687974
(Region: Viet Nam; Strain: Betacoronavirus sp. strain VZ_BetaCoV_20724_43, complete genome.; Date: 12-Nov-14)
Gene
nucleocapsid protein
Description
Annotated in NCBI,
nucleocapsid protein
Location
GenBank Accession
Full name
Nucleoprotein
Alternative Name
Nucleocapsid protein
Sequence
CDS
ATGTCTCATACTCCAGGTAAAGCTGGTAGCAGATCATCCTCTGGGAACCGTAATGGCGGCATTCTTAAGTGGGCCGACCAGACGGAACCTAATAATGTTAATAGCAGAGGTAGAAGAGCCCAACCTAAGCAAACAGCTAACACCCAGGCTTCAGGAGGAAATGTTGTACCATTTTACTCCTGGTTCTCTGGAATTACCCAGTTTCAAAAAGGGCAAGAATTTGGATTTGAACAAGGACAAGGTGTGCCCCTTACCAATGGTGTCCCTTCCTCAGAAGAAAAGGGATACTGGTACAGGCACAACAGAAAGTCCTATAGAACAGCTGATGGACAGCAACGGCAGCTGCTACCCAGATGGTATTTTTACTATCTCGGAACAGGGCCGTATGCGAACAAACAATACGGGTCGGATATCGACGGTGTTGTTTGGGTTGCTAGTCCAAATGTTGATGTACGTTCCCCTGCGGATGTTCCAGATAGGGATCCTACTAGCCACTCAGCTGAGCCGACTAGGTTTGCGCCTGGCACGGTTCTGCCTAAAGGCTATTATGTTGAAGGCTCAGGAAGGTCTGCTCCTAGCTCGCGATCTAGCTCTCGTGCGTCCAGCAGAGCGTCTAGTGTCGGTGGCAGCAGAAGCAGAAACAACTCAGCTTCTAGAAATAATACCTCTGAAGTGACACCTGATATGGCTGATCAGATTGCTTCCCTTGTGCTTGCTAAGCTGAACAAAGAGTCCGGCAAACCAGTGCAAGTCACAAAGCAGACAGCCAAGGAAATAAAACAGAAAATTCTCAACAAACCCCGCCAAAAGCGGACGCCCAATAAGCAGTGCACTGTTCAACAGTGTTTCGGCAAAAGGGGCCCCAGCCAGAATTTTGGTGGTGGTAGCATGCTTAAATTGGGAACTAGTGATCCACAGTTCCCCATCCTTGCAGAATTGGCCCCCACAGCGGGAGCCTTTTTCTTTGGGTCCAGGTTGGAATTGGCCAAAGTTAATAATATGTCTGGTGCTGCTGATGAGCCTCAGAAGGATGTTTATGAGCTGCGCTATTCTGGTGCAATAAGATTTGATAGCACCCTACCTGGGTTTGAGACTATCATGAAAGTTATGAATGAAAATTTGGATGCGTACCAACACACAGAAGAGGGTGCGCAATTATTGAGCCCAAAATTTCAGCGCAAGCAGAAGAAAGGGCAAGTTGAAAAGGATAATGTGAGTGCTGCAACGCCCAAGAGTCGTGTGCAGCAAAATGTAAGAAGAGAGCTAACTCCCCAGGACCTCAGCATTCTCGCGCAGATTGATGATGATACCCAGGGAAGCTCAGAAATATAA
Protein
MSHTPGKAGSRSSSGNRNGGILKWADQTEPNNVNSRGRRAQPKQTANTQASGGNVVPFYSWFSGITQFQKGQEFGFEQGQGVPLTNGVPSSEEKGYWYRHNRKSYRTADGQQRQLLPRWYFYYLGTGPYANKQYGSDIDGVVWVASPNVDVRSPADVPDRDPTSHSAEPTRFAPGTVLPKGYYVEGSGRSAPSSRSSSRASSRASSVGGSRSRNNSASRNNTSEVTPDMADQIASLVLAKLNKESGKPVQVTKQTAKEIKQKILNKPRQKRTPNKQCTVQQCFGKRGPSQNFGGGSMLKLGTSDPQFPILAELAPTAGAFFFGSRLELAKVNNMSGAADEPQKDVYELRYSGAIRFDSTLPGFETIMKVMNENLDAYQHTEEGAQLLSPKFQRKQKKGQVEKDNVSAATPKSRVQQNVRRELTPQDLSILAQIDDDTQGSSEI
Summary
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Subunit
Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
Similarity
Belongs to the betacoronavirus nucleocapsid protein family.
Uniprot
Pubmed
EMBL
Pfam
PF00937
Corona_nucleoca
ProteinModelPortal
PDB
4LMC
E-value=3e-59 Score= 190 Identity=74.24%
Cov(Q)=29.80% Cov(P)=97.78%
3D Structure
Ontologies
GO
Subcellular Location
From MSLVP
Host nucleus > host nucleolus. Host cytoplasm. Secreted.
From Uniprot
Host Golgi apparatus
Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Virion Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Virion Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. With evidence from 1 publications.
Topology
Length:
443
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.11685
Exp number, first 60 AAs:
0.01859
Total prob of N-in:
0.00457
outside
1 - 443
