Strain
Human_IBV_MH779860
(Region: USA; Strain: Infectious bronchitis virus isolate Ark99_pathogenic_field_virus, complete genome.; Date: 2014)
Gene
1a polyprotein
Description
Annotated in NCBI,
1a polyprotein
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCCTCAAGCCTAAAACAGGGAGTATCTCCCAAACCAAGGGATGTCATTCTTGTTTCCAAAGACATTCCCGAACAACTCTGTGACGCTTTATTCTTTTATACGTCACACAACCCTAAGGATTACGCTGATGCTTTTGCCGTTAGGCAGAAGTTTGACCGTAGTCTGCAGACTGGGAAGCAGTTCAAATTTGAAACTGTGTGTGGTCTCTTCCTCTTGAAGGGAGTTGACAAAATAACGCCTGGCGTCCCAGCAAAAGTTTTAAAAGCCACTTCTAAGTTGGCAGATTTAGAAGACATTTTTGGTGTCTCTCCTTTTGCACGGAAGTACCGTGAGTTGTTGAAAACAGCATGCCAGTGGTCTCTTACTGTAGAAACACTGGATGTTCGTGCACAAACGCTTGATGAAATTTTTGACCCCACTGAAATACTTTGGCTTCAAGTGGCTGCAAAAATTCAAGTTTCAGCTATGGCAATGCGCAGGCTTGTTGGAGAAGTAACTGCAAAAGTCATGGAAGCTCTTGGCTCAAATTTGAGTGCTCTTTTTCAAATTGTTAAACAACAAATAGCCAGAATCTTCCAAAAGGCACTGGCTATTTTTGAAAATGTGAGTGAATTACCACAGCGTATTGCAGCACTTAAGATGGCCTTTGCCAAGTGTGCCAAGTCAATTACTGTTGTGGTTGTGGAAAGAACTCTAGTTGTTAGAGAGTTCGCAGGAACTTGTCTTGCAAGCATCAATGGTGCTGTTGCAAAATATTTTGAAGAACTTCCAAATGGCTTTATGGGTTCTAAAATCTTCACAACATTGGCCTTCTTTAAAGATGCAGCTGTGAAAATTGTGGACAATATACCAAATGCACCAAGAGGCACTAAAGGGTTTGAAGTCATTGGCAATGCTAAGGGTACACAAGTTGTTGTGCGTGGCATGCGAAATGACCTAACACTGCTTAATCAAAAAGCTGACATCCCTGTGGAGCCAGAAGGCTGGTCTACAATCTTGGAGGGACATCTTTGTTATGTCTTTAAGAGTGGGGACCGTTTTTATGCGGCACCTCTTTCTGGAAACTTTGCATTGCATGATGTACATTGCTGTGAGCGTGTTGTCTGTCTTTCTGATGGTGTAACACCGGAGATAAATGATGGACTCGTTCTTGCCGCAATTTACTCATCCTTCAGTGTGTCAGAACTCGTGGCGGCCCTTAAAAAAGGCGAACCATTTAAGTTCTTAGGCCACAAATTTGTGTATGCAAAGGATGAAGCAGTGTCGTTTACTTTAGCGAAGGCTGCCACTATTGCAGATGTTTTGAAACTGTTTCAATCAGCTCGTGTGCAAGCAGAAGATATTTGGTCTGCATTTACTGAAAAGTCTTTCAATTTTTGGAAGCTCGCATATGGAAAAGTGCGTAATCTTGAAGAATTTGTTAAGACTCATTATTGTAAGGCTCAAATGTCGATTGTGGTTCTAGCAGCAGTGCTTGGAGAAGGCATTTGGCATCTTGTATCGCAGGTCATCTATAAACTAGGTGGTCTTTTCACTAAAATTGTTGATTTCTGTGAGAAATACTGGAAAGGGTTTTGTGCACAGTTGAAAAAGGCTAAGCTTATTGTGACTGAAACTCTTTGTGTTCTAAAAGGAGTTGCACAGCACTGTTTCCAACTATTGCTGGATGCAATACATTCTCTGTATAAGAGTTTTAAGAAGTGTGCACTTGGTAGAATTTATGGAGATTTGCTCTTCTGGAAAGGGGGTGTACACAAAATTGTTCAAGATGGCGATGAAGTTTGGTTTGACGCCATTGATAGTATTGATGTTGAAGATTTGGGTGTTGTTAAAGAAAAACCCATAGATTTTGAGGTTTGTGAAGACGTAACACTTCCAGAAAAGCAACCTGGTCATATGGTTCAAATCGAAGAAGACGGAAAGAACTATATGTTCTTCCGCTTCAAAAAGGATGAGAACATCTATTATACACCAATGTCTCAACTTGGTGCAATTAATGTAGTTTGCAAAGCAGGCGGTAAAACCGTCACCTTTGGAGACACTACTGTGAAAGAAATACCGCCACCTGATGTTGTTCCTATTAAGGTTAGCATCGAGTGCTGTGGTGAACCATGGAATACAATCTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAGACAGACCTTACAGTTGAACAATTGCTTTCTGTGATCTATGAGAAAATGTGTGATGACCTCAATTTGTTTCCAGAGGCACCTGAGCCTCCACCATTTGAGAACGTTGCGCTTGTTGATAAGCACGGTAAAGACTTGGATTGCATAAAATCATGCCATCTTATCTACCGTGACTATGAGAGTGATGATGACATCGAGGAGGACGATGCTGAGGAGTGTGATACTGCCTCAGCTGATGTTGAAGAATGTGACACAACCTCAGAATGTGAAGAAGAGGACGAGGATACTAAAGTGTTGGCTCTTATACAAGACCCGGCAAGTAACAAGTATCCTTTACCGCTTGATGATGATTATAGCGTCTACAATGGTGCAATTGTACATAAGGACGCTCTTGATGTTGTGAATCTACCATCTGGTGAAGAAGCCTTTGTCATTAATAATTGTTTTGAAGGAGCTGTTAAACCACTCCCACAGAAAGTTGTTGATGTTTTAGGTGACTGGGGTGCGGCTGTTGATGCACAAGAACAATTGTGTCAACAGGAACCTACTCAAGTCATACTTGAAAAATCAGTTGAGTATTTTACTGGTGGTTGTGAGGCAATGGCTGAACGAGCTGTCTTTGAAGAACAGGAGGTAGTTTCTGTATTTGAAGAAAAGTCTGAGGTGATTTTTTGCACACCTTCAGACCTAGAAGTTGCTAAAGAAACAGCAGAAGAATTTGATGAGTTCATTCTCATTTCTGATGTTCCTAAAGAAGAAGTTGTGTCTCAAGAGAAAGTGGAGCCACAAGTTGAGCAAGAGCCTATTCAAGTTGTTAAATCACAACGTGAGAAGAAGGCTAAAAAATTCAGAGTTAAACCAGCCACATGCGAAAAACCCAAGTTTTTGGAGTATAAAACCTGTGTGGGTGATTTAACTGTTGTAATTGCCAAAGCACTGGACGAGTTTAAAGAGTTCTGTGTTGTAAACGCCGCAAATGAGCATATGTCTCATGGTGGAGGCGTTGCGAAGGCAATTGCAGACTTTTGTGGACCTGATTTTGTGGAATATTGCGAGGACTATGTTAAGAAACATGGGCCACAGCAGAGACTTGTTACACCCTCGTTTGTTAAAGGTATTCAATGTGTGAATAATGTTGTGGGGCCACGCCATGGAGACATTAACTTGCATGATAAGCTTGTTGCTGCTTACAAGAATGTTCTTGTAGATGGCGTCTTCAATTATGTTGTGCCAGTTCTCTCATCAGGGATCTTTGGTGTAGATTTTAGAATGTCAATAGACGCAATGCGTGAAGCCTTTAATGGTTGCGACATACGCGTCCTCCTGTTTTCTCTGAGTCAAGAACACATTGAATATTTCGATGCAACTTGCAAGCAGAAGACACTTTATCTTACGGAGGATGGTGTTAAATACCGCTCCGTTGTTGTAAAACCTGGCGACTCTTTGAGTCAATTTGGACAGGTTTTTGCTAAGAATAAGGCAGTCTTTACAGCTGATGATGTTGAGGATAAAGAAATCCTCTTCGTTCCTACTACTGACAAGACTGTTCTTGATTATTATCATTTAGATGCGCAAAAGTATGTGATATATTTACAAACTCTTGCGCAGAAATGGGATGTCCAATATAGAGACAATTTTGTTATATTAGAGTGGCGTGATGGAAACTGCTGGATTAGTTCAGCAACAGTGCTCCTCCAAGCTGCTAAGGTAAAGTTTAGGGGTTTTCTTGCAGAAGCATGGGCTAAGTTCTTGGGTGGAGATCCCACGGATTTTGTGGCCTGGTGTTATGCGAGTTGCAATGCTAAAGTTGGTGAGTTTTCAGATGCCAATTGGCTTTTGGCAAATTTGGCAGAATATTTTGATGCAGACTATACGAACGCACTTCTTAAGAAGCGTGTGTCTTGTAACTGCGGTGTAAAGAGTTATGAACTTAGAGGCCTTGAAGCTTGTATACAACCAGTAAGGGCACCTAACCTTCTACATTTTAAGACGCAATACTCAAATTGCCCAACCTGTGGTGCAAACAGTACGGATGAAGTAATAGAAGCTTCCTTACCATACTTATTGCTCTTTGCTACTGATGGTCCTGCTACAGTAGATTGTGATGAAAATGCTGTAGGGACTGTTGTATTCATAGGCTCTACTAATGGTGGCCATTGTTATACACAAGCTGTTGGAAAAGCTTTTGATAATTTGGCTAAGGATAGAAAATTTGGAAAGAAGTCGCCTTACATTACAGCAATGTATATGGGCTTTTCTCTTAAGAGTGAAAATCCTCTACCTGTTGTTAAACAGACTAAGGGTAAAACTAAGGTAGTAAAAGAAGATGTTTCTAACCTCGCTACCAGTTCCAAAGTCAGTTTTGATAATCTTACTAGCTTTGAACAGTGGTATGATAGTAACATCTATGAAAGTCTTAAAGTGCAGGAAACACCTGACAATTTTGATGGGTATGTGTCATTCACAACAAAGGAAGATTCTAAGTTGACACTGACACTTAAAGTTAGAGGTATTAAATCAGTTGTTGACTTTAAGTCGAAGGATGGTTTTACTTACACGTTAACACCTGACACCGATGAAAATTCAAAAGCACCAGTCTACTACCCAATCTTAGACTCTATTAGTCTCAAGGCAATTTGGGTGGAAGGTAGTGCTAATTTTGTTGTTGGTCATCCAAATTATTATAGTAAGTCTCTGCGCATTCCTACTTTTTGGGAAAATGCTGAGAACTTTGTTAAAATAGGTGAAAAAAATGATGGTGTAACTATGGGCCTTTGGCGTGTAGAACATCTTAATAAACCTAATTTGGAGAGAATTTTCAACATAGCTAGGAAAGCTATTGTTGGATCTAGTGTTGTTACTACACATTGTGGTAAATTAGTCGGTAAAGCGGCTACATTCATTGCTGATAAAGTAGGTGGTGGTGTAGTTCGCAACATTACAGATAGAATTAAGGGTCTTTGTGGAATTACACGTGGGCATTTCGAAAGAAAGATGTCCCCACAATTCATAAAAACACTTATATTCTTTTTCTTTTACTTTGTAAAGGCTAGCGCTAAGAGTGTGGTTGCTAGCTACAAGAGTGTGTTATCTAAGGTGGTGTTTACCACGTTATTTATATTATGGTTTATCTACACAAGTAATCCAGTAATGTTTACTGGAATACGTGTGTTAGACTTCTTATTTGAGGGTTCTTTATGTGGTCCTTATAATGACTATGGTAAAGATTCTTTTGACGTGTTACGCTATTGTGCAGATGATTTTGTTTGTCGTGTTTGTTTACATGGCAGAGATTCTCTTCATTTGTATAAAAATGCTTATAGCGTAGAACAGGTTTATAAAGCTGCAAGTTCTGGCATTAGTTTTAATTGGAATTGGCTTTATTTGGTCTTTCTAATACTGTTTGTAAAGCCAGTGGCAGGTTTTGTTATTATTTGCTATTGTGTTAAGTATTTAGTGTTGAGCTCAACTGTTCTGCAAACTGGTGTAGGCTTTTTGGACTGGTTTGTTCAGACAGTTTTTACTCACTTTAACTTTATGGGTGCAGGGTTTTATTTCTGGCTCTTTTATAAGATATATATACAGGTGCAGCATATACTGTATTGTAAGGATATAACATGTGAAGTGTGCAAGAGAGTTGCACGCAGCAACAGGCAGGAGGTTAGCGTGGTTGTTGGTGGACGCAAACAAATAGTGCATGTCTACACCAACTCTGGCTATAACTTTTGTAAGAGACATAATTGGTATTGCAGAAACTGTGATGACTATGGTCACCAAAACACATTTATGTCTCCAGAAGTTGCTGGCGAACTTTCGGAAAAGCTCAAGCGCCATGTTAAACCTACAGCATATGCATATCACGTTGTGGATGAGGCATGCTTAGTTGATGATTTTGTTAATTTAAAATACAAAGTTGCAGTTCCCGGTAAGGATAGTGCATCTCCTGCTGTTAAGTGTTTCAGTGTTACAGATTTCTTAAAGAAAGCTGTTTTTCTTAAGGAAGCATTGAAATGTGAACAAATATCTAATGATGGTTTTATAGTTTGTAATACACAGAGTGCGCATGCATTAGAGGAAGCAAAGAATGCAGCCATCTATTATGCGCAATATTTGTGTAAGCCAATACTTATACTTGACCAGGCACTTTATGAGCAATTAATAGTAGAGCCTGTGTCTAAGAGTGTTATAGATAAAGTGTGTAGCATTTTGTCTAACATAATATCTGTAGATAATGCAGCTTTAAATTACAAGGCAGGCACACTTCGTGATGCTCTGCTTTCTGTTACTAAGGATGAAGAAGCCGTAGATATGGCTATCTTCTGTCATAATCATGATGTGGAATATACTGGTGATGGTTTTACTAATGTGATACCGTCATATGGTATAGACACTGATAAATTAACACCTCGTGATAGAGGTTTCTTGATAAATGCAGATGCTTCTATTGCTAACTTAAGAGTTAAAAATGCTCCACCGGTAGTATGGAAGTTCTCTGATCTTACTAAGTTGTCTGACGGTTGTCTTAAATATTTAATTTCAGCTACTGTCAAGTCAGGATGTCGTTTCTTTATAACAAAGTCTGGTGCTAAACAAGTTATTTCTTGTCATACCCAGAAGTTGCTGGTAGAGAAAAAGGCAGGTGGTGTTGTTAACAACACTTTTAAATGGTTTAAGAGTTGTTTTAAATGGCTCTTTGTCTTTTATATACTTTTTACAGCATGTTGTTTGGGTTACTATTATATGGAGATGAATAGAAGTTTTGCTCATCCCATGTATGATGTTAACTCTACATTGCATGTTGAGGGTTTTAAAGTTATAGATAAAGGTGTTCTCAGGGAAATTATGCCAGAAGACACATGTTTCTCTAATAAGTTTGTTAATTTTGATAGTTTCTGGGGTAAACCATATGATAATAGTAGAAACTGTCCAATTGTTACAGCTGTTATAGATGGCGATGGGACAGTAGCAGCTGGTGTTCCTGGTTTTGTATCATGGGTTATGGATGGCGCTATGTTTGTACATATGACACAGACTGAGCGAAAACCTTGGTACATTCCTACTTGGTTTAATAGAGAAATTGTAGGTTACACTCAGGATTCAATTATTACTGAGGGTAGTTTTTATACATCTATAGCGTTATTTTCCGCTAGGTGTTTATATTTAACAGCCAGCAATACACCTCAATTGTATTGTTTTAATGGTGATAATGATGCACCTGGAGCCTTACCATTTGGTAGTATTATGCCTCATAGGGTATATTTTCAACCTAATGGTGTTAGGCTTATAGTCCCACAACAAGTACTGCACACACCCTATGTAGTGAAGTTTGTTTCAGACAGCTATTGTAGAGGTAGTGTGTGTGAGTATACTAAACCAGGTTACTGTGTGTCATTGAACTCTCAATGGGTTCTGTTTAATGATGAATACACAAGTAAACCTGGCGTTTTTTGTGGTTCTACTGTTAGAGAACTTATGTTTAATATGGTTAGCACATTTTTTACTGGTGTCAACCCTAATATTTATATGCAGCTAGCAACTATGTTCTTAATATTAGTTGTTGTTGTGTTGATCTTTGCAATGGTTATAAAGTTTCAAGGTGTCTTTAAAGCTTATGCGACCATTGTTTTTACAATAATGTTAGTTTGGGTTATTAATGCATTTGTTTTGTGTGTACATAGTTATAACAGTGTTTTAGCTGTTATATTACTAGTACTCTATTGTTATGCATCACTGGTTACAAGTCGTAATACTGCTATAATAATGCATTGTTGGCTTGTTTTCACCTTTGGTTTAATAGTACCCATGTGGTTAGCTTGTTGCTACCTAGTATACTTTATTTATATGTACACACCGTTGGTTGTATGGTGTTATGGTACTACTAAAAATACTCGTAAACTGTATGATGGCAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGCACTTTCGTTATTCGCGGTTCTGAGTTTGTTAAGCTTACGAATGAGATAGGTGATAAATTTGACGCCTATCTCTCAGCGTATGCTAGACTTAAATATTATTCAGGTACTGGCAGTGAGCAGGATTACTTGCAAGCCTGTCGTGCATGGTTAGCTTATGCTTTGGACCAATATAGAAACAGTGGTGTTGAAATTGTGTATACTCCACCACGTTACTCTATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAACTAGTTTTTCCTAGTAGTGCTGTTGAAAAGTGCATTGTTAGTGTCTCTTATAGAGGTAATAATCTTAATGGACTGTGGCTAGGTGATACTATCTACTGTCCGCGACATGTTCTAGGCAAGTTTTCAGGTGATCAATGGAGTGATGTACTTAATCTTGCTAATAATCATGAGTTTGAGGTTGCAACTCAAAATGGTGTTATTTTGAATGTTGTTAGTAGGCGGTTGAGAGGGGCAGTTTTAATTTTACAAACTGCTGTCGCCAACGCTGACACTCCTAAGTATAAGTTTGTTAAAGCTAATTGTGGTGATAGTTTCACTATAGCTTGTTCTTATGGTGGTACAGTTGTGGGACTCTACCCTGTTACTATGCGTTCTAATGGTACTATTAGAGCTTCTTTCCTTGCAGGAGCTTGTGGCTCAGTTGGTTTTAATATAGAAAAGGGTGTAGTTAATTTCTTTTATATGCACCATCTTGAGTTACCTAATGCATTACACACTGGAACTGACCTATTGGGTGCTTTCTATGGTGGTTATGTGGACGAAGAGGTTGCACAAAGGGTGCCACCAGATAATTTAGTTACTAACAATATTGTAGCATGGCTTTATGCCGCAATTATTAGTGTTAAGGAGAGTAGTTTCTCACTGCCTAAATGGTTGGAGAGTACTACTGTCAGTGTTGAAGATTACAATAAGTGGGCTGGTGATAATGGTTTTACACCATTTTCTACTAGTACTGCTATTACTAAATTAAGTGCTATAACGGGAGTAGATGTTTGTAAACTCCTTCGCACTATTATGGTAAAAAGTAGTCAATGGGGTAGTGATCCCATTTTAGGACAATATAATTTTGAAGATGAATTGACACCAGAGTCTGTTTTTAACCAGATAGGTGGTGTTAGGTTACAGTCATCTATTGTAAGAAGAGCCACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGCTTCTTATTTGTGTTGTGTGCTATTGTCTTGTTTACGGCAGTACCACTTAAATACTATGTACATGCAGCTGTTATTTTGTTAACAGCTGTACTTTTTATTTCTTTTACTGTTAAACATGTTATGGCATATATGGATACTTTTCTGTTGCCTACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTCCCTTTCATATACAATACTCTAATTAGTCAAGTTGTTATTTTCTTAAGCCAATGGTATGATCCTGTAGTCTTTGATACTATGGTACCATGGATGTTATTGCCATTAGTGTTGTACACTGCTTTTAAGTGTGTACAAGGTTGCTATATGAATTCTTTCAATACTTCTTTGTTAATGCTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTACACCTCTTCAAACACTCTTACTGCATATACAGAATGTAATTGGGAGTTGTTCTTTGAGTTGGTTCACACTAATGTGTTGGCTAATGTTAGTAGTAATTCCTTAATTGGTTTAATTGTTTTTAAGTGTGCCAAGTGGATGTTGTATTATTGCAATGCAACATACTTTAACAATTATGTGTTAATGGCAGTCATGGTCAATGGCATAGGCTGGCTTTGCACCTGTTACTTTGGATTGTATTGGTGGGTCAATAAGGTTCTTGGTTTAACCTTAGGTAAATACAATTTTAAAGTCTCAGTAGATCAATATAGGTATATGTGTTTGCATAAGATAAACCCACCTAAAAATGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGAATTGGTGGTGATCGTGTGTTGCCTATTGCTACAGTTCAATCTAAACTGAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATCCTTGCATCTGATGATGTTGGAGAGTGCATGGATAATTTGTTGGGTATGCTTATTACACTATTTTGTATAGATTCTACTATTGATTTGAGTGATTATTGTGATGATATACTTAAGAGGTCAACTGTCTTACAGTCAGTTACTCAAGAGTTTTCACACATACCCTCTTATGCTGAATATGAAAGAGCTAAGAATCTTTATGAAAAGGTTTTAGCTGATTTTAAAAATGGTGGTGTAACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCAGTTTTTGATAGAGACTTGGCTGTTCAAAAGAAGTTAGACAGCATGGCAGAACGTGCTATGACAACAATGTATAAAGAGGCGCGTGTGACTGATAGACGAGCAAAATTAGTTTCATCACTACATGCGTTACTTTTTTCAATGCTTAAGAAAATAGATTCTGAAAAGCTTAATGTTTTATTTGATCAGGCTAGTAGTGGTGTTGTACCTCTAGCTACTGTTCCAATTGTTTGTAGTAATAAGCTTACCCTTGTAATACCAGATCCAGAAACTTGGGTCAAGTGTGTAGAGGGTATGCATGTTACATATTCAACAGTTGTTTGGAATATAGACACTGTTATTGATGCCGATGGTACAGAGTTACATCCAACTTCTATAGGTAGTGGATTGACATACTGTATAAGTGGTGACAATATAGCATGGCCTTTAAAGGTCAACTTGACTAGGAATGGGCATAATAAGGTTGATGCTGTTTTGCAGAATAATGAGCTTATGCCTCATGGTGTAAAAACAAAGGCTTGCGTAGCAGGTGTAGATCAAGCACATTGTAGCGTAGAGTCTAAATGTTATTATACAAATATTAGTGGCAATTCAGTTGTAGCTGCTATTACTTCTTCAAATCCAAATCTGAAAGTAGCTTCGTTTTTGAACGAGGCAGGCAATCAGATTTATGTAGACTTAGACCCACCATGTAAATTTGGCATGAAGGTGGGTGACAAGGTTGAGGTTGTTTACTTGTATTTTATAAAGAATACAAGGTCAATTGTTAGGGGTATGGTACTTGGTGCTATATCTAATGTTGTTGTCTTACAGTCTAAAGGGCATGAAACAGAGGAAGTGGATGCTGTTGGCATTCTTTCACTTTGCTCATTCGCAGTAGATCCCGCTGATACATATTGTAAATATGTGGCAGCAGGTAATCAACCTTTAGGTAACTGTGTTAAAATGTTGACAGTACATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGTCCCACTCCTGATCAGGATTCTTATGGAGGAGCCTCTGTGTGTCTCTATTGTAGAGCACACATAGCGCATCCAGGTGGAGTAGGAAATTTAGATGGACGTTGTCAATTTAAAGGTTCTTTTGTGCAAATACCTACTACGGAGAAAGATCCTGTTGGGTTCTGTCTACGTAATAAGGTCTGCACTGTCTGTCAGTGTTGGATTGGTTATGGGTGCCAGTGTGATCTACTCAGACAACCAAAATCTTCTGTTCAATCAGCTGCTGGTGCATCTGATTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSPKPRDVILVSKDIPEQLCDALFFYTSHNPKDYADAFAVRQKFDRSLQTGKQFKFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVETLDVRAQTLDEIFDPTEILWLQVAAKIQVSAMAMRRLVGEVTAKVMEALGSNLSALFQIVKQQIARIFQKALAIFENVSELPQRIAALKMAFAKCAKSITVVVVERTLVVREFAGTCLASINGAVAKYFEELPNGFMGSKIFTTLAFFKDAAVKIVDNIPNAPRGTKGFEVIGNAKGTQVVVRGMRNDLTLLNQKADIPVEPEGWSTILEGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLSDGVTPEINDGLVLAAIYSSFSVSELVAALKKGEPFKFLGHKFVYAKDEAVSFTLAKAATIADVLKLFQSARVQAEDIWSAFTEKSFNFWKLAYGKVRNLEEFVKTHYCKAQMSIVVLAAVLGEGIWHLVSQVIYKLGGLFTKIVDFCEKYWKGFCAQLKKAKLIVTETLCVLKGVAQHCFQLLLDAIHSLYKSFKKCALGRIYGDLLFWKGGVHKIVQDGDEVWFDAIDSIDVEDLGVVKEKPIDFEVCEDVTLPEKQPGHMVQIEEDGKNYMFFRFKKDENIYYTPMSQLGAINVVCKAGGKTVTFGDTTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYEKMCDDLNLFPEAPEPPPFENVALVDKHGKDLDCIKSCHLIYRDYESDDDIEEDDAEECDTASADVEECDTTSECEEEDEDTKVLALIQDPASNKYPLPLDDDYSVYNGAIVHKDALDVVNLPSGEEAFVINNCFEGAVKPLPQKVVDVLGDWGAAVDAQEQLCQQEPTQVILEKSVEYFTGGCEAMAERAVFEEQEVVSVFEEKSEVIFCTPSDLEVAKETAEEFDEFILISDVPKEEVVSQEKVEPQVEQEPIQVVKSQREKKAKKFRVKPATCEKPKFLEYKTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSFVKGIQCVNNVVGPRHGDINLHDKLVAAYKNVLVDGVFNYVVPVLSSGIFGVDFRMSIDAMREAFNGCDIRVLLFSLSQEHIEYFDATCKQKTLYLTEDGVKYRSVVVKPGDSLSQFGQVFAKNKAVFTADDVEDKEILFVPTTDKTVLDYYHLDAQKYVIYLQTLAQKWDVQYRDNFVILEWRDGNCWISSATVLLQAAKVKFRGFLAEAWAKFLGGDPTDFVAWCYASCNAKVGEFSDANWLLANLAEYFDADYTNALLKKRVSCNCGVKSYELRGLEACIQPVRAPNLLHFKTQYSNCPTCGANSTDEVIEASLPYLLLFATDGPATVDCDENAVGTVVFIGSTNGGHCYTQAVGKAFDNLAKDRKFGKKSPYITAMYMGFSLKSENPLPVVKQTKGKTKVVKEDVSNLATSSKVSFDNLTSFEQWYDSNIYESLKVQETPDNFDGYVSFTTKEDSKLTLTLKVRGIKSVVDFKSKDGFTYTLTPDTDENSKAPVYYPILDSISLKAIWVEGSANFVVGHPNYYSKSLRIPTFWENAENFVKIGEKNDGVTMGLWRVEHLNKPNLERIFNIARKAIVGSSVVTTHCGKLVGKAATFIADKVGGGVVRNITDRIKGLCGITRGHFERKMSPQFIKTLIFFFFYFVKASAKSVVASYKSVLSKVVFTTLFILWFIYTSNPVMFTGIRVLDFLFEGSLCGPYNDYGKDSFDVLRYCADDFVCRVCLHGRDSLHLYKNAYSVEQVYKAASSGISFNWNWLYLVFLILFVKPVAGFVIICYCVKYLVLSSTVLQTGVGFLDWFVQTVFTHFNFMGAGFYFWLFYKIYIQVQHILYCKDITCEVCKRVARSNRQEVSVVVGGRKQIVHVYTNSGYNFCKRHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHVKPTAYAYHVVDEACLVDDFVNLKYKVAVPGKDSASPAVKCFSVTDFLKKAVFLKEALKCEQISNDGFIVCNTQSAHALEEAKNAAIYYAQYLCKPILILDQALYEQLIVEPVSKSVIDKVCSILSNIISVDNAALNYKAGTLRDALLSVTKDEEAVDMAIFCHNHDVEYTGDGFTNVIPSYGIDTDKLTPRDRGFLINADASIANLRVKNAPPVVWKFSDLTKLSDGCLKYLISATVKSGCRFFITKSGAKQVISCHTQKLLVEKKAGGVVNNTFKWFKSCFKWLFVFYILFTACCLGYYYMEMNRSFAHPMYDVNSTLHVEGFKVIDKGVLREIMPEDTCFSNKFVNFDSFWGKPYDNSRNCPIVTAVIDGDGTVAAGVPGFVSWVMDGAMFVHMTQTERKPWYIPTWFNREIVGYTQDSIITEGSFYTSIALFSARCLYLTASNTPQLYCFNGDNDAPGALPFGSIMPHRVYFQPNGVRLIVPQQVLHTPYVVKFVSDSYCRGSVCEYTKPGYCVSLNSQWVLFNDEYTSKPGVFCGSTVRELMFNMVSTFFTGVNPNIYMQLATMFLILVVVVLIFAMVIKFQGVFKAYATIVFTIMLVWVINAFVLCVHSYNSVLAVILLVLYCYASLVTSRNTAIIMHCWLVFTFGLIVPMWLACCYLVYFIYMYTPLVVWCYGTTKNTRKLYDGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFDAYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEIVYTPPRYSIGVSRLQAGFKKLVFPSSAVEKCIVSVSYRGNNLNGLWLGDTIYCPRHVLGKFSGDQWSDVLNLANNHEFEVATQNGVILNVVSRRLRGAVLILQTAVANADTPKYKFVKANCGDSFTIACSYGGTVVGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFFYMHHLELPNALHTGTDLLGAFYGGYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSLPKWLESTTVSVEDYNKWAGDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMVKSSQWGSDPILGQYNFEDELTPESVFNQIGGVRLQSSIVRRATSWFWSRCVLACFLFVLCAIVLFTAVPLKYYVHAAVILLTAVLFISFTVKHVMAYMDTFLLPTLITVIIGVCAEVPFIYNTLISQVVIFLSQWYDPVVFDTMVPWMLLPLVLYTAFKCVQGCYMNSFNTSLLMLYQFMKLGFVIYTSSNTLTAYTECNWELFFELVHTNVLANVSSNSLIGLIVFKCAKWMLYYCNATYFNNYVLMAVMVNGIGWLCTCYFGLYWWVNKVLGLTLGKYNFKVSVDQYRYMCLHKINPPKNVWEVFSTNILIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLITLFCIDSTIDLSDYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKNLYEKVLADFKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVIPDPETWVKCVEGMHVTYSTVVWNIDTVIDADGTELHPTSIGSGLTYCISGDNIAWPLKVNLTRNGHNKVDAVLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGVGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDLLRQPKSSVQSAAGASDFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 673 Identity=94.41%
Cov(Q)=9.06% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3953
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
394.67682
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00708
outside
1 - 1726
TMhelix
1727 - 1746
inside
1747 - 1752
TMhelix
1753 - 1775
outside
1776 - 1845
TMhelix
1846 - 1868
inside
1869 - 1887
TMhelix
1888 - 1910
outside
1911 - 2282
TMhelix
2283 - 2300
inside
2301 - 2559
TMhelix
2560 - 2582
outside
2583 - 2586
TMhelix
2587 - 2609
inside
2610 - 2615
TMhelix
2616 - 2638
outside
2639 - 2652
TMhelix
2653 - 2675
inside
2676 - 3097
TMhelix
3098 - 3120
outside
3121 - 3124
TMhelix
3125 - 3142
inside
3143 - 3154
TMhelix
3155 - 3177
outside
3178 - 3191
TMhelix
3192 - 3214
inside
3215 - 3225
TMhelix
3226 - 3248
outside
3249 - 3257
TMhelix
3258 - 3280
inside
3281 - 3299
TMhelix
3300 - 3322
outside
3323 - 3953
