Strain
Avian_MK581202
(Region: Poland; Strain: Infectious bronchitis virus strain gammaCoV/Ck/Poland/80/1989, complete genome.; Date: 8-Jun-89)
Gene
polyprotein 1a
Description
Annotated in NCBI,
polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTTCAAGCCTAAAACAGGGAGTATCTCCCAAATCAAGGGATGTCGTTCTTGTTGCTAAAGACATTCCCGAACAACTCTGTGACGCTTTGTTTTTCTATACGTCACACAACCCTAAGGATTACGCTGATGCTTTTGCCTGCAGGCAGAAGTTTGATCGTAACCTTCAGACTGGGAAGCAGTTCAGATTTGAAACTGTGTGTGGTCTCTTCCTCTTGAAGGGAGTTGATAAAATAACACCTGGTGTCCCAGCGAAAGTTTTGAAAGCCACTTCTAAGTTGGCTGATTTAGAAGACATTTTTGGTGTATCTCCGCTTGCACGGAAATACCGTGAATTGTTAAAAACAGCATCTCAGTGGTCGCTTACTGTTGCAGCACTGGATGCTCGTGCACAAACTCTTGATGAAATTTTTGACTCCACTGAAATCCTTTGGCTTCAGGTGGCAGCAAAAATTCAAGTTTCAGCTATGGCAATGCGACGGCTCGTTGGAGAAGTAACTGCAAAAGTCATGGATGCTTTGGGCTCAAATTTGAGTGCTCTTTTCCAAATTGTCAAAGAACAAATAGCCAGAATCTTTCAAAAGGCACTGGCTATTTTTGATAATGTCAGTGAATTACCACAGCGTATTTCAGCACTAAAGATGGCTTTTGCTAGGTGTGCAAAATCATTTACAGTTGTGGTCTTTGAGAGAGCTCTCGTTATTAGGGAGTTTGCTGGAACTTGCCTTGCAAGTATTAATGGTGCCATTGCAAAATTCTTTGAAGAACTTCCAACTGGATTTATGGGTGCAAAAATTTTCACAACACTGGCATTCTTTAAAGAGGCTGCTGTGAAAATTGTTGAAAACATACCAAATGCCCCAAGAGGTACCAAAGGTTTTGAAGTTGTTGGCAATGCTAAGGGAACACAAGTTGTTGTGCGTGGCATGCGAAATGACATAACTTTGCTCGACCAAAGAGCTGACATTCCTGTTGAGAAAGAGGGTTGGTCTGCAATTTTGGAAGGACATCTTTGCTATGTCTTTAGAAGTGGTGATCGGTTTTATGCTGCACCTCTTTCAGGAAATTTTGCATTGCATGATGTACATTGCTGCGAGCGTGTAGTTTGTCTGTCAGATGGTGTAACACCGGAGATAAATGATGGGCTCATTCTCGCTGCAATATACTCATCTTTCAGTGTCACAGAGCTTGTGGCTGCACTTAAAAAGGGTGAGCCATTCAAATTTTTGGGACACAAGTTTGTTTATGCAAAGGATGCAGCAGTCTCGTTTACACTAGCGAAGGCTGCAACTATAGCAGATGTTTTGAAGCTGTTTCAATCAGCTCTTGTAAAAGTAGAAGACATTTGGTCTTCGCTTACTGAAAAGTCTTTTGAATTCTGGAAGCTTGCGTATGGAAAAGTGCGCAACCTCGAAGACTTTTTGAAGACTCATGTTTGTAAGGCCCAAATGTCAATTGTCATCCTAGCCGCAGTGCTTGGTGAAGGCATTTGGCATCTTGTCTCTCAAGTCATCTATAAATTGGGTGGTCTTTTTACCAAGGTTGTTGATTTTTGTGAAAAACAGTGGAAAGGTTTCTGTGCACAGTTGAAAAGAGCTAAGCTCATTGTGACTGAAACACTTTGTGTTCTGAAAGGAGTTGCAGAGCATTGTTTTCAACTACTGCTGGATGCAATACATTCTATGTACATGAGTTTTAAGAAGTGTGCACTTGGTCGAATCCATGGAGACTTGTTCTTCTGGAAAGGAGGTGTGCATAAAATTGTTCAAGATGGTGATGAAATATGGTTTGACGCCATCGATAGCATTGATGTTGAAGATCTGGGTGTTGTTCAAGAAAAACCCATAGATTTTGAGGTTTGCGAAGACGTGACACTTGCAGAAAATCAACCTGGTCACATGGTTCAAATTCATGATGACGGACAGAACTATATGTTCTTCCGCTTCAAGAAGGATGAGAACATTTACTACACACCAATGTCTCAACTTGGTGCTATTAATGTTGTTTGCAAAGCGGGCGGAAAAACCGTCACCTTTGGAGAAACTACTGTTCAAGAAATCCCACCACCTGATGTCGTGCCTATCAAGGTCAGCATTGAGTGTTGTGGTGAGCCATGGAACACTATCTTCAAGAAGGCTTACAAAGAGCCCATTGAAGTTGAAACTGGCCTCACAGTAGAACAATTGCTCTCTGTGATCTATGAGAAAATGTGTGACGATCTCAAGTTGTTTCCTGAGGCCCCAGAACCTCCACCTTTTGAGAATGTCGCACTTGTAGATAAGAATGGTAAAGATTTGGATTGTATAAAATCCTGTCATCTTATTTACCGTGATTATGAGAGCGATGATGACATCGAGGAAGAAGATGCTGAGGAGTGTGACACAGACTCAGGTGAGGCTGAAGAGTGTGACACTAGTTCAGAATGTGAACAGGAGGACGAGGCTACTAAGGTGTTGTCTCTTATACAAGACCCAGCAAGTAATAAATACCCCCTTCCTCTTGATGACGACTATAGCGTTTACAATGGATGCATTGTACATAAAGACGCTCTTGATGTTGTGAATCTACCATCTGGTGAAGAAACTTTTGTTGTCAATAACTGTTTTGATGGAGCAGTTAAACCGCTGCCACAAAAAGTTATTGACGTTCTCGGTGATTGGAGTGAGGCTGTTGATGCACAAGAACAACTGTGTCAACAGGAACACACTCAAGACAATTCTGAAGGACCAGTGGAAAATTCTACTGGTGATTCTAGTGTGTCGACTGAACAAGTTGTTGTTGAAGAGCAGGAATTAATTCCCGTTGTTGAAGAAGAACAAGCGGTAGTTGTCTATACCCCTGCTGAGCTACAAGTTGTTGATGAAACACCAGATGAGTTTATTCTCACTGCTGATGTTCCTACAGAAGAATTTGTGTCTCAAGAAGAAAAGGAGCCACAGGTCGAACAAGAGCCTGTTCAGGTTGCTAAATCGCAACGTGAAAAGAAGGCTAAAAAGTTCAAGGTTAAACTAACTACATGTGAAAAACCCAAATATTTGGAGTATAAAACATGTGTAGGTGATTTGACTGTAGTAGTTGCCAAAGCATTGGATGAGTTTAAAGAATTCTGCCTTGTAAACGCTGCAAATGAGCATATGTCTCATGGGGGCGGTGTTGCAAAGGCAATTGCAGACTTTTGTGGACCAGATTTTGTAGAATACTGTGAGGACTATGTTAAGAAACATGGGCCACAGCAGCGACTTGTCACACCTTCATATGTTAGAGGCATTCAATGTGTGAACAATGTTGTTGGACCACGCCATGGAGACAGTAACTTGGAGGATAAACTCGTTGCTGCATACAAGAATGTGCTTGTAGATGGCGTTGTAAATTATGTTGTTCCTGTTCTCTCATCAGGAATCTTTGGTGTAGACTTTAAATTGTCAATAGACGCAATGCGCAAAGCCTTTGAGGGTTGTGACATACGCGTTTTGCTTTTCTCTCTGAGTCAAGAACACATCAATTATTTCGATGTAACTTGTAAACAGAAGACAATTTATCTTACAGAGGATGGTGTGAAATATCGCTCTGTTGTTTTAAAACCTGGAGACTCTTTGGGTCAATTTGGACAGGTTTTTGCAAAGAATAAGACAGTTTTTACAGCGGATGATGTAGATGACAAAGAAATCCTCTTCATTCCTACGACTGATAAGGCTGTTCTTGAATATTATGGTTTAGATGCGCAAAAGTATGTGATATATTTGCAAACGCTTGCGCAGAAATGGAATGTCCAATATAGAGACAATTTTGTTATACTAGAGTGGCGTGATGGAAACTGCTGGGTTAGTGCAGCAATAGTGATTCTTCAAGCTGCTAAAATTAGGTTTAAAGGTTTTCTTGCAGAAGCATGGGCTAGACTGTTGGGTGGAGACCCTACAGATTTTGTAGCATGGTGCTATGCAAGTTGCAATGCTAAAGTTGGTGATTTTTCAGATGCTAATTGGCTCTTGGCCAACTTGGCAGAATATTTTGATGCAGATTATACGAACGCACTTCTTAAGAAGCGCGTGTCGTGTAACTGTGGTATTAAGAATTATGAACTTAGAGGCCTTGAAGCTTGTATTCAACCGGTAAGGGCACCTAACCTTCTACATTTTAAGACGCAATATTCAAATTGCCCAACCTGTGGTGCAAATAGTATGGATGAAGTTGTTGAAGCTTCACTACCATACTTATTGCTCCTTGCTACTGATGGCCCTGCTACAGTTGACTGTGATGAGAGTGCTGTAGGGACTGTTGTTTTCATTGGCTCTACTAATAGTGGCCATTGCTACACACAAGCCATTGGTAAGGCTTTTGATAATCTTGCTAAGGATAGAAAATTTGGAAAGAAGTCGCCTTACATTACAGCAATGTATACGCGATTCTCTCTCAAGAGTGAAAATCCTCTGCCTGTTATTAAACGGAGTAAGGATAAAACTAAAGTAGTAAAAGAAGATGTTTCTAACCTTGCCACTAGTTCTAAGGTCAGTTTTGATGATCTTGCTGAATTTGAACGTTGGTATGATAGTAACATCTACGAAAGTCTTAAAGTGCAGGAAACACCTGAGGATTTGGATGATTATGTGTCATTTACAACGAAGGAAGATTCCAAGTTGCCACTGACACTTAAAGTTAGGGGTATCAAATCAGTTGTAGACTTTAAGTCAAAGGATGGCTTTACCTATAAGTTAATACCTGATACTGAAGAAAATTCAAAAGTACCAATCTACTATCCTGTCTTGGATTCTATTAGTCTTAAGGCTATATGGGTAGAAGGTACTGCTAATTTTGTAGTTGGACATCCTAACTATTATAGTAGGTCCCTTCGCATTCCTACTTTCTGGGAGTGTGCTGAGAGCTTTGTTAAAATAGGGGAAAAAGTTGATGGTGTAACTACAGGCTTATGGCGTGCAGAACACCTTAATAAACCTAATTTGGAGAGAATTTTCAACATTGCTAAGAAAGCAATTGTTGGAACTAGTGTTGTTACTACAGAATGTGGTAAATTAATAACTAAAGCAGCTACATTTATTGCCGATAAAGTAGGCGGTGGTGTGGTTGGTAATATTACAGATAGAATTAAGGGTATTTGTGGATATACACGTGGGCATTTTGAGAATAAGATGTCCCCACAATTCATAAAAACACTTATGTTCTTCTTCTTTTATCTTATAAAGGCTAGTGCTAGGAATTTAATCGCTAGCTACAAGACTGTATTACGTAAGGTGGCATTTGCCTCTTTACTTATGTTTTGGTTTGTATACACAAGTAAACCAGTAATGTTTACTGGAATACGTATATTAGACTTTCTATTTGAAGGTTCTTTCTGTGGTCCTTATAATGACTATGGTAAAGATTCTTTTGATGTGTTACGCTATTGTGAAGATGATTTTATTTGTCGTGTGTGTTTATCTGATAGAGATTCACTCCATTTGTATAAACATGCCTATAGCGTAGAACAGTTATATAAAGATGCATCTTCTGGCATAGGTTTTAATTGGAATTGGCTTTATTTAGTCTTTCTAATATTGTTTGTTAAACCAGTAGCAGGTTTTGTTATTATTTGCTATTGTGTTAAGTATTTGGTATTGAATTCAACTGTGTTGAACACCGGTGTAGGTTTTCTAGACTGGTTTGTTCAGACAGTCTTTACTCACTTTAATTTTATGGGCGCAGGGTTCTATTTCTGGCTCTTTTATAAGATTTACATACAGGTGCATCACATACTTTATTGTAAGGATGTAACATGTGAAGTTTGTAAGCGTGTTGCGCGTAGCAATAGATATGAGGTTAGTGTAGTCGTTGGCGGGCGTAAACAGCTTGTGCATGTCTACACTAACTCTGGCTATAACTTTTGTAAGAGACATAATTGGTATTGTAAGAATTGTGATATTTATGGTCATCAAAATACATTTATGTCCCCAGAAGTTGCTGGTGAGCTTTCTGATAAGCTTAAACGTCATGTTAAACCTACAGCATATGCTTACCACGTTGTGGATGAGGCATGCGTAGTGGATGATTTTGTTAATTTAAAATACAAAGCTGCAGTTCCTGGTAAGGATAATGCATCTCCTGCTGTTAAGTGCTTCAGTGTTACAGACTTCTTGAAGAAGGCTGTGTTCCTTAGGGACGCACTGAAATGTGAACAGATATCTAATGATGGTTTTATAGTGTGTAATACACAAAGTGCACATGCATTAGAGGAAGCTAAGAATGCTGCCATCTATTATGCGCAATATTTGTGTAAACCAATACTTATACTTGACCATGTACTCTATGAGCAATTAGTTGTAGAGCCTGTATCAAAGAGTGTTGTAGATAAGGTGTGTGATATCTTGTCTAATATAATTTCTGTAGATACTGCATCTTTAAATTATAAGGCAGGCACACTACGTGATTGTCTTCTTACTGTTACTAAAGATGAAGAGGCTGTAGATATGGCTATTTTCTGTCACAATCATGAAGTGGAATACACTGGTGACGGTTTTACTAATGTAATACCATCATATGGTGTAGACACTGATAAATTGACCCCCCGAGATAGAGGGTTTTTGATAAATTCAGATGCTTCCGTCGCCAACTTAAGAGTTAAAAATGCTCCACCGGTAGTATGGAAGTTCTCTGATATTACTAAATTGTCTGACAGTTGCCTTAAATATTTAATTCCAGCTACTGTCAAATCAGGATGTCGTTTCTTTATAACGAGGTCTGGTGGTAAACAAGTATTCTCTTGTGCTACTCAGAAATTGTTAGTAGAGAAAAAAGCCGGTGGCATTGTTAGTGACACCATTAGTAAGATTAAGAGTTGTTGTAAATGGCTCTTGGTCTTTTATGTTCTTTTTACAGTGTGTTGTTTGGGTTTTTACCACATGGAAATGAATAAAAGTTTTGTTCACCCCATGTATGATGTAAACTCTACAATGCATGTTGAAGGTTTTAAGGTTATAGATAAAGGTGTTATTAGAGAAATTGTGTCAGAAGATACATGTTTCTCTAATAAGTTTGCTAATTTTGATGCCTTTTGGGGTAAACCATATGAAAATAGTAGAAACTGTCCAATTGTAACAGCCGTCATTGATGGTGTTGGGACAGTAGCTGCTGGTGTTCCTGGTTATGTAGACTGGGTTATGGATGGTGTTATGTTTATACACATGACACAGACAGAGCGAAAACCCTGGTACATTCCTACTTGGTTTAATAGAGAAATTGTTGGTTACACTCAGGATTCAATTGTTACTGAGGGTAGTTTCTATACATCTGTAGCATTATTTGCAGCTAGGTGTTTATATTTAACAGCCAGCAATACACCTCAATTGTATTGTTTTAATGGTGACAATGATGCACCTGGAGCCTTACCATTTAGTGATATACTCCCTCATAGGGTGTATTTCCAACCTAATGGTGTAAGACTTATAGTCCCTCAACAAATAATGCACACACCATACATAGTAAAGTTTATATCAGACAGCTATTGTAGAGGTAGTGTATGTGAGTATACTAAACCAGGTTACTGTGTGTCATTAAACTCTCAATGGGTTTTGTTTAATGATGAATACACAGGTAAACCAGGCATCTTTTGTGGTTCTACTGTTAGAGAACTTATTTTTAATATGGTTAGCACATTCTTTACTGGTGTCAACCCTAATATATATATGCAACTAGCAACTATGTTCTTAATACTAGTTGTTGTTGTTTTAATCTTTGCAATGGTTATAAAGTTCCAAGGTGTTTTTAAAGCTTATGCGACCATTGTGTTTACAATAGTGCTAGTTTGGTTTATTAACGCATTTATTTTGTGTGTGCATAGTTATAATAGTGTTGTAGCTATTATATTACTAGTACTTTATTGTTATTTATCATTGGTAACAAGTCGCAATACTTCTATAATAATGCATTGCTGGCTTGTTTTCACTTTTGGTTTAATAATACCTACATGGTTAGCCTGTTGCTACCTAGGGTTTATTTTCTATATGTACACGCCATTGTTTTTCTGGTGTTATGGTACTACTAAAAATACTCGTAAGCTGTATGAAGGCAATGAGTTTGTTGGTAATTATGACCTTGCTGCGAAGAGCACTTTTGTTATTCGTGGTTCGGAATTTGTTAAGCTTACGAACGAGATAGGTGATAAGTTTGAACACTATCTCTCAGCGTATGCTAGACTTAAATACTACTCAGGTACAGGCAGTGAGCAAGATTATTTGCAAGCTTGTCGTGCTTGGCTAGCTTATGCTTTAGACCAATTTAGAAACAGTGGTGTGGAAATTGTTTATACACCACCACGTTACTCTATTGGTGTTAGCAGGTTACAGGCTGGTTTTAAGAAACTGGTTTCTCCTAGTAGTGCTGTTGAAAGGTGCATTGTTAGTGTGTCATATAGAGGCAATAATCTTAATGGACTGTGGCTAGGCGACACTATCTACTGTCCTCGTCATGTATTAGGTAAGTTTTCAGGTGACCAGTGGAATGATGTACTTAACCTTGCTAATAATTATGAGTTTGAAATTACAACTCAAAATGGTGTTACTTTGAATGTTGTCAGCAGGCGCTTAAGAGGTGCAGTTTTGATATTACAAACTGCAGTAGCCAATGCTGAAACACCTAAGTATAAGTTTTTGAAGGCTAATTGTGGTGATAGTTTCACTATAGCTTGTTCTTATGGTGGTGCAGTTGTGGGACTATACCCTGTTACTATGCGTTCTAATGGTACCATTAGGGCTTCGTTCCTTGCAGGAGCTTGTGGCTCAGTTGGTTTTAATATAGAAAAGGGTGTAGTTAATTTCTTTTATATGCACCATCTTGAGTTACCTAATGCATTACACACCGGAACTGACCTAATGGGAGAGTTCTATGGTGGTTATGTTGATGAAGAGGTTGCACAAAGGGTGCCACCAGATAACTTAGTTACTAACAATATTGTAGCATGGCTTTATGCTGCAATTATTAGTGTTAAGGAGAGTAGTTTCTCACTGCCTAAATGGTTGGAGAGTACTACTGTCAGTGTTGAAGATTATAATAAGTGGGCTAGCGATAATGGTTTTACACCGTTTTCCCCTAGCGCTGCTATTACTAAATTAAGTGCTATAACGGGAGTAGATGTTTGTAAACTCCTTCGCACTATTATGGTAAAAAGTAGTCAATGGGGTAGTGACCCCATTTTAGGACAATATAATTTTGAGGATGAATTGACACCAGAGTCTGTTTTTAACCAGATAGGTGGTGTTAGGTTACAGTCTTCTATTGTAAGAAGAGCTACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGCTTCTTATTTGTGTTGTGTGCTATGGTCTTGTTCACGGCTGTGCCACTTAAGTTTTATGTACATGCAGCTGTTATTTTGTTAACAGCTGTGCTCTGTGTTGCTTTTACTGTTAAACATGTTATGGCGTTTATGGATACGTTTCTATTGCCTACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTTCCCTTCATATACAATACTCTAATTAGTCAAGTTGTTATTTATGTTAGCCAATGGTATGATCCTGTAGTGTTTGATACTATGGTACCATGGATGTTACTACCATTAGTGTTGTATACTGCTTTTAAGTGTGTACAAGGTTGTTATATGAATTCTTTCAATACTTCTTTGTTAATGCTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTATACCTCTTCAAACACTCTTACTGCATATACAGAAGGTAATTGGGAGTTGTTTTTTGAATTGGTTCATACTACGGTGTTGGCTAATGTTAGTAGTAATTCTTTAATAGGTTTATTTGTATTTAAGTTAGCTAAGTGGATGTTGTATTATTGTAATGCTACATACTTTAATAATTATGTGCTAATGGCAGTCATGATTAATTGCATTGGCTGGCTTTGTACTTGCTATTTTGGACTTTATTGGTGGATTAATAGGGTTTTTGGTTTAACCCTAGGTAAGTACAGTTTTAAAGTTTCAGTAGAACAATATAGGTACATGTGTCTTCATAAGATACCACCACCTAAGAGTGTTGTGGATATTTTGTCGACAAATATGCTTATACAAGGAATAGGTGGTGATCGTGTGTTACCTATTGCCACGGTTCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTAGAAGCAAATTCAAAGATGCATGCTTATCTTGTTGAGTTACACAATAAAATCCTAGCTTCTGACGATGTTGGAGAGTGCATGGATAATTTGTTGGGTATGCTTATTACATTATTCTGTATAGATTCTACTATTGATTTGAGTGTGTATTGTGATGATATACTTAAGAGGTCAACTGTTTTACAGTCAGTTACTCAAGAGTTCTCACATATACCCTCTTATGCTGAATATGAAAGAGCTAAGAATCTTTATGAAAAGGTTTTAGCTGATTCTAAAAATGGTGGTGTAACACAGCAAGAACTTGCTGCTTATCGTAAAGCTGCCAATATTGCAAAGTCAGTCTTTGATAGAGATTTGGCTGTACAGAAGAAATTAGATAGCATGGCAGAGCGTGCTATGACAACAATGTATAAAGAAGCTCGTGTAACTGACAGAAGAGCAAGATTGGTCTCATCGTTACATGCGCTACTCTTTTCAATGCTTAAGAAAATAGATTCTGAAAAGCTTAATGTACTATTTGACCAGGCGAGTAGTGGTGTTGTACCCTTAGCCACTGTCCCAATTGTGTGTAGTAACAAGCTTACACTTGTAGTACCAGACCCAGAGACGTGGGTTAAGTGCGTAGAAGGTATGCATGTTACTTATTCAACAGTTGTTTGGAATATAGACACTGTTATTGATGCTGATGGCACAGAATTACAACCAACTTCTACAGGTAGTGGTTTGACATACTGTATAAGTGGTGATAATATAGCGTGGCCCTTAAAGGTCAACTTGACTAGGAATGTGCATAATAAGGTCGATGTTTATTTGCAAAATAACGAGCTTATGCCTCATGGTGTAAAAACAAAGGCTTGCGTAGCGGGTGTAGATCAAGCACATTGTAGCGTAGAGTCTAAATGTTATTATACAAATATTAGTGGTAATTCAGTTGTGGCTGCGATTACCTCTTCAAACCCAAATTTGAAAGTTGCATCATTTTTGAATGAGGCAGGCAATCAAATTTATGTAGACTTAGACCCACCATGCAAGTTTGGTATGAAAGTGGGTGATAAGGTTGAAGTTGTTTACCTGTATTTTATAAAGAATACAAGGTCGATTGTTAGAGGTATGGTACTTGGTGCCATATCTAATGTAGTTGTCTTACAATCTAAAGGATATGAAACAGAGGAAGTAGATGCTGTAGGCATTCTTTCCCTTTGTTCTTTTGCAGTAGATCCCGCGGAAACATACTGTAAGTATGTGGCTGCTGGTAATCAACCTTTAGGTAACTGTGTTAAAATGTTGACAGTTCATAATGGTAGTGGTTTTGCTATAACATCAAAGCCAAGTCCAACTCCTGATCAGGATTCTTATGGTGGAGCTTCTGTGTGCCTCTATTGTAGAGCACATATAGCACATCCTGGTAGTGCAGGAAACCTAGATGGGCGTTGCCAACTTAAGGGTTCATTTGTACAAATACCTACTACGGAGAAAGACCCTGTTGGTTTCTGTCTACGTAACAAGGTTTGCACTGTTTGTCAGTGTTGGATAGGTTATGGATGTCAGTGTGACGCACTTAGACAACCCAAACCTTCTGTTCAGTCAGCTGCTGGTGCACCTGATTTTGACAAGAATTATTTAAACGGGTTCGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSPKSRDVVLVAKDIPEQLCDALFFYTSHNPKDYADAFACRQKFDRNLQTGKQFRFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPLARKYRELLKTASQWSLTVAALDARAQTLDEIFDSTEILWLQVAAKIQVSAMAMRRLVGEVTAKVMDALGSNLSALFQIVKEQIARIFQKALAIFDNVSELPQRISALKMAFARCAKSFTVVVFERALVIREFAGTCLASINGAIAKFFEELPTGFMGAKIFTTLAFFKEAAVKIVENIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDITLLDQRADIPVEKEGWSAILEGHLCYVFRSGDRFYAAPLSGNFALHDVHCCERVVCLSDGVTPEINDGLILAAIYSSFSVTELVAALKKGEPFKFLGHKFVYAKDAAVSFTLAKAATIADVLKLFQSALVKVEDIWSSLTEKSFEFWKLAYGKVRNLEDFLKTHVCKAQMSIVILAAVLGEGIWHLVSQVIYKLGGLFTKVVDFCEKQWKGFCAQLKRAKLIVTETLCVLKGVAEHCFQLLLDAIHSMYMSFKKCALGRIHGDLFFWKGGVHKIVQDGDEIWFDAIDSIDVEDLGVVQEKPIDFEVCEDVTLAENQPGHMVQIHDDGQNYMFFRFKKDENIYYTPMSQLGAINVVCKAGGKTVTFGETTVQEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETGLTVEQLLSVIYEKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYESDDDIEEEDAEECDTDSGEAEECDTSSECEQEDEATKVLSLIQDPASNKYPLPLDDDYSVYNGCIVHKDALDVVNLPSGEETFVVNNCFDGAVKPLPQKVIDVLGDWSEAVDAQEQLCQQEHTQDNSEGPVENSTGDSSVSTEQVVVEEQELIPVVEEEQAVVVYTPAELQVVDETPDEFILTADVPTEEFVSQEEKEPQVEQEPVQVAKSQREKKAKKFKVKLTTCEKPKYLEYKTCVGDLTVVVAKALDEFKEFCLVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSYVRGIQCVNNVVGPRHGDSNLEDKLVAAYKNVLVDGVVNYVVPVLSSGIFGVDFKLSIDAMRKAFEGCDIRVLLFSLSQEHINYFDVTCKQKTIYLTEDGVKYRSVVLKPGDSLGQFGQVFAKNKTVFTADDVDDKEILFIPTTDKAVLEYYGLDAQKYVIYLQTLAQKWNVQYRDNFVILEWRDGNCWVSAAIVILQAAKIRFKGFLAEAWARLLGGDPTDFVAWCYASCNAKVGDFSDANWLLANLAEYFDADYTNALLKKRVSCNCGIKNYELRGLEACIQPVRAPNLLHFKTQYSNCPTCGANSMDEVVEASLPYLLLLATDGPATVDCDESAVGTVVFIGSTNSGHCYTQAIGKAFDNLAKDRKFGKKSPYITAMYTRFSLKSENPLPVIKRSKDKTKVVKEDVSNLATSSKVSFDDLAEFERWYDSNIYESLKVQETPEDLDDYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLIPDTEENSKVPIYYPVLDSISLKAIWVEGTANFVVGHPNYYSRSLRIPTFWECAESFVKIGEKVDGVTTGLWRAEHLNKPNLERIFNIAKKAIVGTSVVTTECGKLITKAATFIADKVGGGVVGNITDRIKGICGYTRGHFENKMSPQFIKTLMFFFFYLIKASARNLIASYKTVLRKVAFASLLMFWFVYTSKPVMFTGIRILDFLFEGSFCGPYNDYGKDSFDVLRYCEDDFICRVCLSDRDSLHLYKHAYSVEQLYKDASSGIGFNWNWLYLVFLILFVKPVAGFVIICYCVKYLVLNSTVLNTGVGFLDWFVQTVFTHFNFMGAGFYFWLFYKIYIQVHHILYCKDVTCEVCKRVARSNRYEVSVVVGGRKQLVHVYTNSGYNFCKRHNWYCKNCDIYGHQNTFMSPEVAGELSDKLKRHVKPTAYAYHVVDEACVVDDFVNLKYKAAVPGKDNASPAVKCFSVTDFLKKAVFLRDALKCEQISNDGFIVCNTQSAHALEEAKNAAIYYAQYLCKPILILDHVLYEQLVVEPVSKSVVDKVCDILSNIISVDTASLNYKAGTLRDCLLTVTKDEEAVDMAIFCHNHEVEYTGDGFTNVIPSYGVDTDKLTPRDRGFLINSDASVANLRVKNAPPVVWKFSDITKLSDSCLKYLIPATVKSGCRFFITRSGGKQVFSCATQKLLVEKKAGGIVSDTISKIKSCCKWLLVFYVLFTVCCLGFYHMEMNKSFVHPMYDVNSTMHVEGFKVIDKGVIREIVSEDTCFSNKFANFDAFWGKPYENSRNCPIVTAVIDGVGTVAAGVPGYVDWVMDGVMFIHMTQTERKPWYIPTWFNREIVGYTQDSIVTEGSFYTSVALFAARCLYLTASNTPQLYCFNGDNDAPGALPFSDILPHRVYFQPNGVRLIVPQQIMHTPYIVKFISDSYCRGSVCEYTKPGYCVSLNSQWVLFNDEYTGKPGIFCGSTVRELIFNMVSTFFTGVNPNIYMQLATMFLILVVVVLIFAMVIKFQGVFKAYATIVFTIVLVWFINAFILCVHSYNSVVAIILLVLYCYLSLVTSRNTSIIMHCWLVFTFGLIIPTWLACCYLGFIFYMYTPLFFWCYGTTKNTRKLYEGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFEHYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQFRNSGVEIVYTPPRYSIGVSRLQAGFKKLVSPSSAVERCIVSVSYRGNNLNGLWLGDTIYCPRHVLGKFSGDQWNDVLNLANNYEFEITTQNGVTLNVVSRRLRGAVLILQTAVANAETPKYKFLKANCGDSFTIACSYGGAVVGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFFYMHHLELPNALHTGTDLMGEFYGGYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSLPKWLESTTVSVEDYNKWASDNGFTPFSPSAAITKLSAITGVDVCKLLRTIMVKSSQWGSDPILGQYNFEDELTPESVFNQIGGVRLQSSIVRRATSWFWSRCVLACFLFVLCAMVLFTAVPLKFYVHAAVILLTAVLCVAFTVKHVMAFMDTFLLPTLITVIIGVCAEVPFIYNTLISQVVIYVSQWYDPVVFDTMVPWMLLPLVLYTAFKCVQGCYMNSFNTSLLMLYQFMKLGFVIYTSSNTLTAYTEGNWELFFELVHTTVLANVSSNSLIGLFVFKLAKWMLYYCNATYFNNYVLMAVMINCIGWLCTCYFGLYWWINRVFGLTLGKYSFKVSVEQYRYMCLHKIPPPKSVVDILSTNMLIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLITLFCIDSTIDLSVYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKNLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRARLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWVKCVEGMHVTYSTVVWNIDTVIDADGTELQPTSTGSGLTYCISGDNIAWPLKVNLTRNVHNKVDVYLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGYETEEVDAVGILSLCSFAVDPAETYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSAGNLDGRCQLKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDALRQPKPSVQSAAGAPDFDKNYLNGFGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 676 Identity=90.78%
Cov(Q)=9.06% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3950
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
399.962719999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00515
outside
1 - 1718
TMhelix
1719 - 1741
inside
1742 - 1749
TMhelix
1750 - 1772
outside
1773 - 1840
TMhelix
1841 - 1863
inside
1864 - 1867
TMhelix
1868 - 1887
outside
1888 - 1896
TMhelix
1897 - 1919
inside
1920 - 2279
TMhelix
2280 - 2298
outside
2299 - 2556
TMhelix
2557 - 2579
inside
2580 - 2583
TMhelix
2584 - 2606
outside
2607 - 2609
TMhelix
2610 - 2629
inside
2630 - 2641
TMhelix
2642 - 2664
outside
2665 - 2886
TMhelix
2887 - 2904
inside
2905 - 3094
TMhelix
3095 - 3117
outside
3118 - 3121
TMhelix
3122 - 3139
inside
3140 - 3151
TMhelix
3152 - 3174
outside
3175 - 3188
TMhelix
3189 - 3211
inside
3212 - 3222
TMhelix
3223 - 3245
outside
3246 - 3254
TMhelix
3255 - 3277
inside
3278 - 3296
TMhelix
3297 - 3319
outside
3320 - 3950
