Strain
Avian_MK644086
(Region: China; Strain: Infectious bronchitis virus strain E160_YN, complete genome.; Date: 2018)
Gene
replicase 1a
Description
Annotated in NCBI,
replicase 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTTCAAGCCTAAAACAGGGAGTATCTTCCCAAAACCAAAGGAGTGTCGTTCTTGTTGCCAAAGACATTCCTGACCAACTTCGTGACGCTTTGTTTTTCTATACGTCACATGACCCTAAGGACTACGCTGATGCMTTCTCTTTTAGGCAGAAGTTTGATCGTAGTCTTGGTGCTGGGAAGCAGTTTAAATTTGAAACTGTGTGTGGTTCATTCTGCTTGAAAGGAGTGGACAAAATAACACCAGGTGTCCCAGCCAAAGTTTTAAAAGCCACGTCTACGCTGGCTGATTTAGAAGATATCTTTGGTGTCTCTCCGCTTGCGCGGAAATACCGTGAATTATTGAAAACAGCATGCCAGTGGTCTATGACTGTAGAAGCACTGGATGCTCGTGCACAAACTCTTGATGAAATCTTTGATCCATCTGAGATACTATGGCTCCAAGTGGCTGCAAAGATTCAGGTTTCAGCCATGGCAATGCGCAGACTAGTTGGTGAGACTACTGCAAAAGTCATGGATTCACTTGGCTCAAATTTGAGCGTTCTATTTCAAGTTGTTAAGGAAACAATGGCCAAAATCTTTCAAAAGGCTCTGGCCATTTTTGAGAATGTTAGTGAATTACCACAGCGTATTGCTGCACTTAAAATGGCTTTTGCCAAGTGTGCAAGATCAATTACAGTTGTGGTTGTTGGACATGCTCTCACTATCAGAGAGTTTGCTGGAACTTGTCTTGCAAGCATTAATGGTGCAGTTGCAAAATTCTTTGAAGAACTTCCAACTGGTTTTATGGGTGCTAAGGTCTTTTCAACTCTTGCCTTCTTTAAAGAAGCAGCAGCAAAGATTGTAGAAAATATACCAAATGCGCCAAGAGGTACTAAAGGTTTTGAAGTCGTTGGCAATGCCAAAGGTACACAAGTTGTTGTCCGTGGCATGCGAAACGACTTAACTTTGCTTGACCAAAAAGCTGAAATTCCTGTGGAACCAGAAGGTTGGTCTGCAATTCTGGATGGACATCTTTGCTATGTCTTTAAGAGTGGTGATCGCTTTTATGCGGCACCTCTTTCAGGAAATTTTGCATTGCATGATGTGCATTGCTGCGAGCGTGTAGTCTGTCTGTCTGATGGTGTAACACCGGAGATAAATGACGGGCTTGTTCTTGCTGCAATTTACTCCTCTTTTAATGTTTTGGAACTTGTGGCAGCACTTAAAAAAGGTGAACCATTCAAGTTCTTGGGGCACAAATTTGTTTATGCAAAGGAAGCCGCAGTTTCTTTTACTTTGGCAAAAGCAGCAACCATTGGAGATGTCCTGAAGCTGTTTCAATCAGCAAGAGTAAAGACGGAAGATGTGTGGTCTGCACTTACTGAAAAGTCTTTTGAATTCTGGAAACTTGCGTACAGAAGAGTGCGCAATCTTGAAGAATTTCTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTGTGATTTTAGCAGCAGTGCTTGGTGAGGGCATTTGGCATCTTTGTTCACAAGTCATCTATAAATTGAGTGGCTTGTTTTCCAAAGTCGTTGACTTTTGTGAAAAACATTGGAAAGGTTTCTGCACACAGTTGCGAAAGGTTAGGCTTGTTGTTACAGAAAACCTTTGTGTGTTGAAAGGAATTGCTCAGCATTGTTTTCAACTTTTGCTGGAAGCAATACATTCTATGTACAAGAGTTTTAAGAAATGTGCACTTGGTAGAGTTTCTGGGGATTTATTTTTCTGGAAAGGCGGTGTGCATAAAATTGTGCAGGATGGCGATGAAATTTGGTTTGACGCCATTGATACTATTGAAGTTGAAGATCTGGGTGTTGTCCAAGAAAAACCTATAGATTTTGAGGTTTGCGAGGAAGTGACACTTCCAGAAAATCAACCTGGCCACATGGTTCAAATCCAAGATGATGGAAAGAACTACATGTTCTTCCGCTTTAAAAAGGATGAGAACATTTATTATACACCAATGTCACAACTTGGTGCTATTAATGTTATTTGCAAAGCGGGTGGCAAGACTGTCACTTTTGGTGAAACTACTGTAAAGGAAATACCACCACCTGATGTTGTGCCTATTAAGGTAAGCATTGAGTGTTGTGGTGAACCATGGAATACCATCTTCAAGAAGGCTTATAAAGAGCCCATTGAAGTTGAGACAGACCTCACAGTAGAACAATTGCTTACTGTGATTTATGATAAAATGTGTGAAGATCTCAAACTGTTTCTAGAGGCACCTGAACCTCCACCATTTGAGAATGTTGCACTTGTTGATAAAAACGGTAAGGATTTGGATTGCATAAAATCATGCCATCTCATCTACCGTGATTATGAGAGCGATGATGACATCGAGGAAAAAGATGCTGAGGAGTGTGACACAGAGTCAGCTGATGCTGAGGAATGTGACACTGCTTCAGAGTGTGAAGAAGAAGATGAGGATACAAAGGTGCTGTCTCTTTTGCAAGACCCAGCCAGTAACAAGTACCCTCTACCCCTCGATGAAGATTATAGCGTCTACAATGGATGTATTGTTCATAAGGATGCTCTTGATGTTGTTAACTTACCATCTGGTGAAGAAACTTTTGTTGTCAATAACTGTTTTGACGGAGCTGTGAAACCGCTTCCGCAGAAGGTTATTGACGTTCTTGGAGATTGGGGAGAGGCCGTTGATGCACAAGAACAATTGTGTGAACAAGAGCCTGAACCTGTTAAAGAATTAGTTGAGAAACCTATTGGTGTTGGTGGATCCGCAATCGTTGTAGAGCAGGAAGTAATTCCTATTGTTGAAGAGTCTCAAGAAGTAGTTGTTTTTACACCTGCTGATTTAGAAGTTGTTAAAGAAACATCAGATGATGTCGATGAGTTTTTTCTTGTTGCTGATGTTCCTACAAAAGAAGTTGTGCCTCAAGAAAAAGAGGAGCCACAAGTAGAGCAGGAGCCTATTCAAGTTGTTAAACCACAACGTGAGAAAAAGGCTAAAAAGTTCAGAGTCAGGCAAACCACATGTGAAAAACCCAAATTCTTGGAGTACACAACATGTGTAGGTGATTTGACYGTAGTAATTGCCAAAGCATTGGAYGAATTTAAAGAGTTCTGCGTTGTAAACGCCGCAAATGAGCATATGTCTCATGGTGGTGGCGTTGCAAAGGCAATTGCAGACTTTTGTGGACCTGATTTTGTGGAATATTGTGAGGACTATGTTAAGAAACATGGGCCACAACAAAAACTTGTCACACCTTCGTATGTTAAAGGCATTCAATGTGTGAATAATGTTGTAGGACCCCGCCATGGTGACAAAGATTTGGAGGAAAAACTTGTCGCTGCCTACAAGAATGTTCTTGTAGATGGTGTGGTCAATTATGTTGTGCCAGTTCTCTCATCGGGAATCTTTGGTGTAGATTTTAAAATCTCAATAGACGCGATGCGTAAGGCCTTTGAAGGGCTTAACATACGCGTGCTCTTCTTTTCTTTGAGTCAAGAACACATCGATTATTTCGATGCAACTTGCAAGCAGAAGAAAATTTATCTTACAGAAGATGGTGTTAAGTACCGCTCTGTTGTAGTGAAACCTGGCGACTCTTTGGGTCAATTTGGACAGGTTTTTGCAAAGAACAAGACAGTTTTTACAGCAGATGATGTTGAGGACGAAGAAGTTCTCTTCATTCCAACAACTGACAAAGCTGTTCTTGAATACTATGGATTGGATGCGCAAAAGTATGTAATATATTTGCAAACACTTGCGCAGAAATGGGAAGTCCAATATAGGGACGATTTTATTGTACTTAAGTGGCGTGATGGAAATTGCTGGATTAGTTCAGCAATTGTACTCCTTCAGGCTGCTAAGATTAGGTTTAAGGGTTTTCTTGCAGAAACATGGGCTAAACTGTTGGGTGGAGACCCTACTGAGTTTGTAGCTTGGTGTTATGCAAGTTGCAATGCTAAAGTTGGTGATTTTTCAGACTGCAATTGGCTGTTGGCTAATTTAGCAGAATACTTTGATGCCGACTACACGAATGCGCTTCTTAAGAAGCGTGTGTCGTGTAACTGCGGTGTTAAGAGCTATGAATTTAGGGGCCTTGAAGCTTGTATTCAGCCGGTAAGAGCTCCTAATCTTCTACATTTTAAGACGCAATATTCAAATTGCCCAACATGTGGTGCAGATAGCACGGATGAAGTTGTTGAAGCTTCACTCCCGTACCTATTGCTCGTCGCTACTGATGGTCCTGCTGCAGTTGATTGTGATGAAAATGCTGTAGGAAATGTTGTTTTCATTGGGTCCACCAATAGTGGACACTGTTATACACAAGTTGTTGGTAAAGCTTTTGATAATTTGGCTCATGATAGAAAATTTGGAAAGAGGTCGCCGTACATTACTGCAATGTATACGCGTTTCTCTCTTAAGAGTGAAAATTCTCAGCTTATTGCTAAAAAGAGCAAGAGCAAGAGCAAAGTAGTAAAAGAAGATGTTTCTAACCTTGCCACTAGTTCTAARGTCAGTTTTGATGATCTTACTGACTTTGAGCAGTGGTATGATAGCAACATTTATGAATGTCTTAGAGTTCAGGAAACACCTGAGAATTTGGATGGGTATGTGTCATTTACAACAAAGGAAGATTCCAAGTTGCCACTGACRCTAAAAGTTAGAGGCATCAAATCRGTTGTTGACTTTAAGTCAAAAGATGGTTTTACTTATAAGTTAACACCTGACACTGATGAAAATTCAAAAGCACCAGTCTACTACCCTGTTTTGGATTCTGTTAGTCTTAAGGCAATATGGGTTGAAGGCAATGCTAATTTTGTTGTTGGGCATCCCAACTATAATAGTAGAGCTCTACGCGTTCCTACTTTTTGGGAGTGCGCTGAGACTTTTGTTAAAATGGGCGAAAAAGTTGATGGTGTAACTATGGGCCTATGGTGTGTAGAACATCTTAATAAACCTAATTTAGAGAAGATTTTCAACATCGTTAAGAAAACGATGGTTGGAGTAAGTGTTGTTACCACTGAATGTGGTAAATTAATTAGTAAGGCAGCTGCGTTCATCGCTGACAAAGTAGGCGATGGAGTAGTCCGCAATGTTACTGATAGAATTAAGGGTGTTTTTGGACTTACACGTGGGCATTTTGAAAGAAAAATGTCACCACAATTCCTAAAAACACTTATGTTCTTTTTCTTCTATTTAATTAAGGCTAGCATTAAAAGTGTGGTCGCTAGCTATAAGAGTGTGCTATGTAAGGTGGTGTTTACTGCTTTACTTATATTATGGTTTGTTTACACAAGTAGTCCTGTAATGTTTACTGGAATACGTGTGGCGGACTTCTTATTTGAAGGTTCTTTATGCGGTCCTTATAATGACTATGGTAAAGATTCTTTTGATGTACTACGCTATTGTGGAGATGATTTTACGTGTCGTGTTTGTTTATATGACAAGGATTCGCTCCACTTGTACAAACATGCTTATAGTGTAGAACAGTTTTATAAAGATGCAGTTTCTGGCATTAGCTTTAATTGGAATTGGCTCTATTTAGTCTTTCTAATATTATTTGTTAAGCCAGTAGCAGGTTTTGTTATTATTTGTTATTGCGTTAAGTATTTAGTATTGAATTCAACTGTTCTGCAAACTGGTGTAGGATTCCTAGATTGGTTTATTCAAACAGTTTTTACTCACTTTAATTTTATGGGAGCAGGGTTTTATTTCTGGCTCTTTTACAAAATATACGTACAGGTGCATCATATACTGTACTGTAAGGATGTAACATGTGAAGTGTGCAAGAGAGTTGCACGCAGCAATAGGTATGAAGTTAGCGTAGTTGTTGGTGGACGCAAGCAAATTGTGCATGTGTATACTAACTCTGGCTATAGATTTTGTAAGAGTCATAATTGGTATTGTAGGAATTGTGACGAGTATGGTCACCAAAATACATTTATGTCCCTAGAAGTTGCTGGCGAGCTTTCTGAAAAGCTTAAACGCCATGTTAAGCCAACAGCACATGCTTATTATGTTGTGGATGAGGCCTGTTTAGTTGATGATTTTATTAATTTAAAATACAAAGCTGCAACGCCTGGTAAAGATAGTGCACCTCCTGCAGTTAAGTGTTTCAGTGTTACAGATTTCTTGAAGAAAGCTGTTTTTCTTAAGGACGCACTGAAATGTGAACAAATATCTAACGATGGTTTTATAGTGTGTAACACACAGAGTGCGCATGCACTAGAGGAAGCGAAGAATGCTGCCATTTATTATGCGCAATATTTGTGTAAACCAATACTTATACTTGATCAGGCACTATATGAGCAATTAGTGGTGGAACCTGTGTCAAAGAGTGTTGTAGATAAAGTGTGTGACATTTTGTCTAAAATAATTTCTGTTGATACTGCAGCTGTAAATTACAAAGCAGGCACACTTCGTGATGCTCTGCTTTCAGTTACTAAAGACGAAGAAGCTGTTGATATGGCTATCTTTTGTCATAATAATGAAGTTGAATATACAGGTGATGGTTTTACTAATGTAATACCGTCATATGGTATAGACACTGATAAATTAACACCTCGTGATAGGGGGTTTTTAATAAATGCGGATGCTTCTGTTGCTAACCTACGAGTTAAAAATGCTCCGCCGGTAGTATGGAAGTTCTCTGATCTTATTAAGTTGTCAGACAGTTGTCTTAAATATCTAATTTCAGCGACTGTCAAGTCAGGTTGTCGTTTCTTTATAACAAGGTCTGGTGCTAAGCAAGTTATTTCTTGTCATACTCAGAAATTGTTGGTAGAGAAAAAGGCTGGTGGTATCGTTAGTGGTACTTTTAATTGTTTTAAGAGCTGTTGTAAACGGCTCTTGATATTTTATGTATGCTTTACATTGTGTTGTTTGGGCTACTATCATATGGAGATGAATAAAAATTTTGTTCACCCAATGTATGATGTTAATTCTACAATGCATGTTGAAGGTTTTAAGGTTATAGATAAGGGTGTTATCAGAGAAATAGTATCAGAAGACACCTGTTTCTCTAATAAGTATGCTAACTTTGATGCCTTTTGGGGCAGACCATATGAAAATAGTAGAGACTGTCCAATAGTTACAGCCATTATAGATGGTGCTGGAACAGTAGCTGCTGGTGTACCTGGGTTTGTTCAATGGGTCATGGATGGAGTAATGTTCATTCATATGACGCAGACTGAACGAAAACCTTGGTATGTCCCTACTTGGTTTAATAGGGAAATTGTCGGTTACACTCAGGATTCAATTATTACTGAGGGTAGTTTCTATACATCTATTGCTTTGTTTTCAGCTAGATGTTTGTATTTAACTGCTAGCAATACACCTCAATTGTATTGTTTTAACGGTGATAATGATGCTCCTGGAGCCTTACCATTTAGTAGTATAACTCCTCATAGGGTATACTTTCAACCTAATGGTGTTAGGCTTATAATACCTCAACAGATAATGCACACACCCTACATAGTAAAGTTTTTATCTGACAGCTATTGTAGGGGTAGTATATGTGAGTATACTAAACCAGGTTATTGTGTAGCACTAAACTCCCAATGGGTGTTGTTTAATGATGAGTACACAAGTCAGCCAGGTATTTTCTGTGGTTCCACTGTTAGAGAACTTATGTTTAATATGATTAGTACATTTTTTACTGGTGTTAATCCTAATATCTATATGCAATTAGCAACTATGTTTTTAATACTAGTTGTTGTTGTTTTGATTTTTGCAATGGTCATAAAGTTTCAAGGTGTGTTTAAAGCTTATGCAACCACTGTTTTTACAATAATGTTAGTTTGGGTTATTAATGCATTTGTGCTATGCGTACATAGTTACAACAGCGTTTTAGCTGTCATATTACTTGTACTCTATTGCTATGCATCATTGGTAACAAACCGCAATACGTCTATAATAATGCATTGTTGGCTTGTTTTTACTTTTGGTTTAATAGTGCCTACTTGGGTAGCTTGTTGCTACCTAGCGTTTGTGCTCTATATTTACACACCATTGTTTTTCTGGTGTTATGGTACTACTAAAAATTCTCGTAAGCTTTATGATGGCAACGAGTTCGTTGGTAATTATGACCTGGCTGCGAAGAGCACTTTTGTGATTCGTGGTCCTGAATTTGTTAAGCTTACGAATGAGATAGGTGATAAATTTGACGCCTACCTTTCAGCGTATGCTAGACTTAAATATTATTCAGGCACAGGCAGTGAACAGGATTATCTGCAAGCTTGTCGTGCATGGTTAGCTTATGCACTTGACCAATATAGAAATAGTGGTGTAGAATTTGTTTACACACCACCACGTTACTCTATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAGCTAGTTTCTCCTAGTAGTGCTGTTGAAAGGTGCATTGTTAGTGTGTCTTATAGAGGCAATAACCTTAATGGACTGTGGCTTGGTGACACTATTTATTGTCCTCGTCATGTGTTAGGTAAGTACTCAGGTGATCAGTGGGATGATGTACTTAATCTTGCTAATAATCATGAGTTTGAAGTTATAACTCAAAATGGTGTTACTTTGAATGTTGTCAGTAGACGGATAAAAGGTGCAGTTTTGATTTTACAAACTGCCGTCGCTAATGCTGAAACTCCAAAGTATAAGTTTGTGAAGGCCAATTGTGGTGATAGTTTCACCATAGCTTGCTCCTATGGTGGTACAGTTGTTGGACTATACCCTGTTACTATGCGTTCTAATGGCACTATTAGAGCATCTTTTCTTGCAGGAGCTTGTGGTTCTGTAGGTTTCAATATAGAAAAGGGTGTAGTTAACTTTTACTACATGCATCATCTTGAATTGCCTAATGCATTACACACTGGAACTGATCTAATGGGTGTGTTCTATGGTGGTTATGTTGATGAGGAGGTTGCACAGAGAGTGCCACCAGATAATCTTGTTACTAATAACATTGTTGCATGGCTTTATGCTGCAATTATTAGTGTTAAAGAGAGTAGTTTTTCAATGCCTAAATGGCTTGAAAGCACTACTGTCAGTGTTGATGGTTATAATAAGTGGGCTGATGATAATGGTTTTACACCGTTTTCAATTAACACTGCTATTACTAGATTGAGTGCTATAACGGGAGTTGATGTTTGCAAACTCCTACGTACTATTATGGTTAAGTCTAGTCAATGGGGTAGCGACCCAATACTAGGCCAATATAATTTTGAGGATGAACTTACACCAGAAGCTGTTTTTAATCAGGTTGGTGGTGTCAGACTACAGTCTTCTTTTGTGAAAAAAGCTACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGTTTCTTATTTGTGTTGTGTGCTATTGTGTTGTTTACGGCAGTGCCACTTAAATATTATGTGCATGCAGCTGTTGTTTTGTTAGCGGCTGTTACATTTATTTCTTTTACTGTTAAACATGTTATGGCTTATATGGATACTTTCCTATTGCCAACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTACCCTTCATTTACAACACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTAGTGTTTGATACTATGGTACCATGGATGTTCTTACCATTAGTGCTGTACACAGCTTTTAAGTGTGTACAAGGTTGCTATAGTGTAAATTCTTTCAATACTTCTTTGTTAGTTTTGTACCAGTTTATGAAGTTGGGGTTTGTTATTTATACCTCTTCTAATACTGTTACTGCATACTCTGAAGGTAATTGGGAGCTATTTTTTGAATTAGTACACACTACTGTGTTGGCTAATATTAGTAGCAATTCTTTAATTGGTTTATTTGTATTTAAGTTTGCTAAATGGATGCTGTATTATTGTAATGCAACATACTTTAATAATTATGTGCTAATGGCAGTCATGGTTAATGGCTTAGGTTGGCTTTTTACTTGTTACTTTGGGTTCTATTGGTGGATTAATAAGGTTTTTGGTTTAACCTTAGGCAAATATAATTTCAAAGTTTCGGTTGACCAATATAGGTATATGTGTCTTCATAAGATAACACCACCTAAGACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGAATTGGTGGCGATCGTGTGTTGCCTATTTCTACAGTTCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTAGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATTCTTGCCTCTGATGATGTTGGGGAGTGCATGGACAATTTATTGGGTATGCTTGTTACACTATTTTGTGTTGATAGTACTATTGATTTGAGTGAGTATTGTGATGATATACTTAAGAGGTCAACTGTTTTACAGTCAGTTACTCAAGAGTTCTCACATATACCATCTTATGCTGAATATGAAAGAGCTAAACAACTTTATGAGAAGGTGTTAGCTGATTCTAAAAATGGTGGTGTAACACAGCAAGAGCTTGCTGCGTATCGTAAAGCTGCCAATATTGCAAAGTCAGTCTTTGATAGAGACCTGGCCGTTCAGAAGAAGTTAGATAGTATGGCAGAGCGTGCTATGACAACTATGTATAAAGAGGCGCGTGTTACTGATAGAAGAGCAAAATTGGTCTCATCGCTACACGCATTACTGTTTTCAATGCTTAAGAAGATTGATTCTGAAAAGCTTAATGTATTATTTGACCAGGCGAATAGTGGTGTTGTACCACTTGCGACTGTTCCAATAGTTTGTAGTAATAAGCTTACACTTGTAGTACCAGACCCTCAAACGTGGGTCAAGTGCGTAGAAGGTATGCATGTTACATATTCAACAGTAGTCTGGAATATAGACAATGTTATAGATGCTGATGGCACTGAATTACATCCTACTTCTACAGGTAGTGGTTTAACTTACTGTATAAATGGAGATAATATAGCATGGCCTCTTAAGGTAAACTTGACTAGGAATGGGCATAATAAAATTGATGCTGCATTGCAGAACAACGAGCTTATGCCTCATGGTGTAAAAACAAAGGCTTGCGTTGCAGGTGTTGATCAAGCACACTGTAGCGTTGAGTCTAAATGTTATTATACAAATATTAGTGGTAATTCAGTTGTAGCTGCTATTACCTCTTCTAATCCAAATTTGAAAGTTGCTTCATTCCTGAATGAGGCAGGCAATCAAATTTATGTAGACTTAGACCCACCATGTAAATTTGGTATGAAGGTGGGTGACAAGGTTGAAGTTGTTTATTTGTATTTTATAAAGAATACAAGGTCAATTGTTAGAGGTATGGTACTTGGTGCTATATCGAATGTTGTTGTTTTACAATCTAAAGGTCATGAGACAGAGGAAGTAGATGCTGTAGGCATCCTATCTCTTTGTTCATTTGCAGTAGACCCAGCAGACACGTATTGTAAATATGTAGCTGCAGGTAATCAGCCTTTAGGTAACTGTGTTAAAATGTTGACAGTTCATAATGGTAGTGGTTTTGCTATAACATCAAAACCAAGTCCAACTCCTGATCAGGATTCCTATGGAGGAGCTTCTGTGTGCCTATATTGCAGAGCACATATAGCTCATCCAGGTGGTGCAGGAAATTTAGATGGACGTTGTCAGTTTAAAGGTTCTTTTGTACAAATACCTACTACGGAGAGAGATCCTGTAGGATTCTGTCTACGCAATAAGGTTTGTACTGTTTGTCAGTGTTGGATAGGTCATGGATGTCAATGTGATTCACTTAGACAACCAAAACCTTCTGTTCAATCAGATGCTGGTGCACCTGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSSQNQRSVVLVAKDIPDQLRDALFFYTSHDPKDYADAFSFRQKFDRSLGAGKQFKFETVCGSFCLKGVDKITPGVPAKVLKATSTLADLEDIFGVSPLARKYRELLKTACQWSMTVEALDARAQTLDEIFDPSEILWLQVAAKIQVSAMAMRRLVGETTAKVMDSLGSNLSVLFQVVKETMAKIFQKALAIFENVSELPQRIAALKMAFAKCARSITVVVVGHALTIREFAGTCLASINGAVAKFFEELPTGFMGAKVFSTLAFFKEAAAKIVENIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEIPVEPEGWSAILDGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLSDGVTPEINDGLVLAAIYSSFNVLELVAALKKGEPFKFLGHKFVYAKEAAVSFTLAKAATIGDVLKLFQSARVKTEDVWSALTEKSFEFWKLAYRRVRNLEEFLKTHFCKAQMSIVILAAVLGEGIWHLCSQVIYKLSGLFSKVVDFCEKHWKGFCTQLRKVRLVVTENLCVLKGIAQHCFQLLLEAIHSMYKSFKKCALGRVSGDLFFWKGGVHKIVQDGDEIWFDAIDTIEVEDLGVVQEKPIDFEVCEEVTLPENQPGHMVQIQDDGKNYMFFRFKKDENIYYTPMSQLGAINVICKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLTVIYDKMCEDLKLFLEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYESDDDIEEKDAEECDTESADAEECDTASECEEEDEDTKVLSLLQDPASNKYPLPLDEDYSVYNGCIVHKDALDVVNLPSGEETFVVNNCFDGAVKPLPQKVIDVLGDWGEAVDAQEQLCEQEPEPVKELVEKPIGVGGSAIVVEQEVIPIVEESQEVVVFTPADLEVVKETSDDVDEFFLVADVPTKEVVPQEKEEPQVEQEPIQVVKPQREKKAKKFRVRQTTCEKPKFLEYTTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQKLVTPSYVKGIQCVNNVVGPRHGDKDLEEKLVAAYKNVLVDGVVNYVVPVLSSGIFGVDFKISIDAMRKAFEGLNIRVLFFSLSQEHIDYFDATCKQKKIYLTEDGVKYRSVVVKPGDSLGQFGQVFAKNKTVFTADDVEDEEVLFIPTTDKAVLEYYGLDAQKYVIYLQTLAQKWEVQYRDDFIVLKWRDGNCWISSAIVLLQAAKIRFKGFLAETWAKLLGGDPTEFVAWCYASCNAKVGDFSDCNWLLANLAEYFDADYTNALLKKRVSCNCGVKSYEFRGLEACIQPVRAPNLLHFKTQYSNCPTCGADSTDEVVEASLPYLLLVATDGPAAVDCDENAVGNVVFIGSTNSGHCYTQVVGKAFDNLAHDRKFGKRSPYITAMYTRFSLKSENSQLIAKKSKSKSKVVKEDVSNLATSSKVSFDDLTDFEQWYDSNIYECLRVQETPENLDGYVSFTTKEDSKLPLTLKVRGIKSVVDFKSKDGFTYKLTPDTDENSKAPVYYPVLDSVSLKAIWVEGNANFVVGHPNYNSRALRVPTFWECAETFVKMGEKVDGVTMGLWCVEHLNKPNLEKIFNIVKKTMVGVSVVTTECGKLISKAAAFIADKVGDGVVRNVTDRIKGVFGLTRGHFERKMSPQFLKTLMFFFFYLIKASIKSVVASYKSVLCKVVFTALLILWFVYTSSPVMFTGIRVADFLFEGSLCGPYNDYGKDSFDVLRYCGDDFTCRVCLYDKDSLHLYKHAYSVEQFYKDAVSGISFNWNWLYLVFLILFVKPVAGFVIICYCVKYLVLNSTVLQTGVGFLDWFIQTVFTHFNFMGAGFYFWLFYKIYVQVHHILYCKDVTCEVCKRVARSNRYEVSVVVGGRKQIVHVYTNSGYRFCKSHNWYCRNCDEYGHQNTFMSLEVAGELSEKLKRHVKPTAHAYYVVDEACLVDDFINLKYKAATPGKDSAPPAVKCFSVTDFLKKAVFLKDALKCEQISNDGFIVCNTQSAHALEEAKNAAIYYAQYLCKPILILDQALYEQLVVEPVSKSVVDKVCDILSKIISVDTAAVNYKAGTLRDALLSVTKDEEAVDMAIFCHNNEVEYTGDGFTNVIPSYGIDTDKLTPRDRGFLINADASVANLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGCRFFITRSGAKQVISCHTQKLLVEKKAGGIVSGTFNCFKSCCKRLLIFYVCFTLCCLGYYHMEMNKNFVHPMYDVNSTMHVEGFKVIDKGVIREIVSEDTCFSNKYANFDAFWGRPYENSRDCPIVTAIIDGAGTVAAGVPGFVQWVMDGVMFIHMTQTERKPWYVPTWFNREIVGYTQDSIITEGSFYTSIALFSARCLYLTASNTPQLYCFNGDNDAPGALPFSSITPHRVYFQPNGVRLIIPQQIMHTPYIVKFLSDSYCRGSICEYTKPGYCVALNSQWVLFNDEYTSQPGIFCGSTVRELMFNMISTFFTGVNPNIYMQLATMFLILVVVVLIFAMVIKFQGVFKAYATTVFTIMLVWVINAFVLCVHSYNSVLAVILLVLYCYASLVTNRNTSIIMHCWLVFTFGLIVPTWVACCYLAFVLYIYTPLFFWCYGTTKNSRKLYDGNEFVGNYDLAAKSTFVIRGPEFVKLTNEIGDKFDAYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEFVYTPPRYSIGVSRLQAGFKKLVSPSSAVERCIVSVSYRGNNLNGLWLGDTIYCPRHVLGKYSGDQWDDVLNLANNHEFEVITQNGVTLNVVSRRIKGAVLILQTAVANAETPKYKFVKANCGDSFTIACSYGGTVVGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFYYMHHLELPNALHTGTDLMGVFYGGYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSMPKWLESTTVSVDGYNKWADDNGFTPFSINTAITRLSAITGVDVCKLLRTIMVKSSQWGSDPILGQYNFEDELTPEAVFNQVGGVRLQSSFVKKATSWFWSRCVLACFLFVLCAIVLFTAVPLKYYVHAAVVLLAAVTFISFTVKHVMAYMDTFLLPTLITVIIGVCAEVPFIYNTLISQIVIFFSQWYDPVVFDTMVPWMFLPLVLYTAFKCVQGCYSVNSFNTSLLVLYQFMKLGFVIYTSSNTVTAYSEGNWELFFELVHTTVLANISSNSLIGLFVFKFAKWMLYYCNATYFNNYVLMAVMVNGLGWLFTCYFGFYWWINKVFGLTLGKYNFKVSVDQYRYMCLHKITPPKTVWEVFSTNILIQGIGGDRVLPISTVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKQLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQANSGVVPLATVPIVCSNKLTLVVPDPQTWVKCVEGMHVTYSTVVWNIDNVIDADGTELHPTSTGSGLTYCINGDNIAWPLKVNLTRNGHNKIDAALQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCQFKGSFVQIPTTERDPVGFCLRNKVCTVCQCWIGHGCQCDSLRQPKPSVQSDAGAPGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 649 Identity=86.87%
Cov(Q)=9.07% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3949
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
406.50126
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00273
outside
1 - 1721
TMhelix
1722 - 1741
inside
1742 - 1747
TMhelix
1748 - 1770
outside
1771 - 1838
TMhelix
1839 - 1861
inside
1862 - 1865
TMhelix
1866 - 1885
outside
1886 - 1894
TMhelix
1895 - 1917
inside
1918 - 2278
TMhelix
2279 - 2296
outside
2297 - 2358
TMhelix
2359 - 2381
inside
2382 - 2554
TMhelix
2555 - 2577
outside
2578 - 2581
TMhelix
2582 - 2604
inside
2605 - 2610
TMhelix
2611 - 2628
outside
2629 - 2647
TMhelix
2648 - 2670
inside
2671 - 3092
TMhelix
3093 - 3115
outside
3116 - 3119
TMhelix
3120 - 3139
inside
3140 - 3150
TMhelix
3151 - 3173
outside
3174 - 3192
TMhelix
3193 - 3215
inside
3216 - 3221
TMhelix
3222 - 3244
outside
3245 - 3253
TMhelix
3254 - 3276
inside
3277 - 3295
TMhelix
3296 - 3318
outside
3319 - 3949
