Strain
Avian_MN128086
(Region: Taiwan; Strain: Infectious bronchitis virus strain TW2296/95vac; Date: 1995)
Gene
replicase polyprotein 1a
Description
Annotated in NCBI,
replicase polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1ab
Alternative Name
ORF1ab polyprotein
Sequence
CDS
ATGGCTTCAAGTCTAAAACAGGGAGTATCTTCCCAAAACCAGAGGAGTGTCATTCTTGTTGCCAAAGACATTCCCGACCAACTCCGTGACGCTTTGTTTTTCTATACGTCACACAATCCAAAAGATTACGCTGATGCTTTTGCTGTTAGACAGAAGTTCGACCGTAATCTGGGAGCTGGGAAACAGTTTAAATTTGAAACTGTGTGTGGTCCATTCTTTTTGAAAGGGGTGGACAAAATAACACCAGGTGTCCCAGCCAAAAGTTTGAAGGCCACTGCAAAGTTGGCTGATATAGAGGGCATCTTTGGTGTCTCTCCTCTTGCGCGGAAATACCGTGAATTGTTGAAAACTGCATCACAGTGGTCGTTTAATGTTGAAACCCTGGATGCATGTGCACAAACTCTTGATGAAATTTTTGAACCCACTGAAATACTTTGGCTTCAAGTGGCTTCAAAGATCCAGGTCGCAGCTATGGCAATGCGCAGGCTTGTTGGTGAAGTAACTGCAAAAGTCATGGAGGCTCTCGGCTCAAGTTTGGGTTCTCTTTTTCAAATTGTAAAAGGGACAATGTCCAGGATCTTTCAAAAAGCACTGGCCATTTTCGACAATGTGAGTGAATTACCACAGCGTATCTTAGCACTAAAAATGGCTTTTGCCAAGTGTGCTAAATCAATTACTGTTGTGGTTGTTGAAAGAGCTCTTGTTATTAGAGAGTTTGCGGGAACTTGTCTTGCAAGTATTAATGGTGCCGTTGCTAAATTCTTTGAAGAACTTCCAAACGGCTTTATGGGAGCAAAGGTGTTTTCAACTCTAGCCTTCTTTAAGGAAGCGGCTGTGAAGATTGTTGAGAACATACCTAATGCACCAAGAGGTACTAAGGGTTTTGAAGTTGTTGGCAATGCGAAAGGCACTCAAGTTGTTGTGCGTGGCATGCGAAATGACTTAACTCTGCTTGACCAAAAAGCTGAGATTCCAGTTGAACCAGAAGGTTGGTCTGCAATTTTGGAGGGACATCTTTGCTATGTCTTTAAGAGCGGTGATCGATTCTATGCGACGCCTCTTTCAGGAAATTTTGCATTGCATGATGTTTATTGTTGCGAGCGTGTTGTCTGTCTGACTGATGGTGTTACACCAGAGATAAATGATGGACTCATTCTTGCTGCAATTTATTCATCCTTCAGTATCTCTGAACTTGTGGCTGCACTTAAAAAAGGTGAACCATTCAAGTTCTTGGGACATAAATTTGTGTATGTGAAGGAAGCAGCGGTTTCTTTCACTTTGGGAAAAGCAGCAACTATTGCTGATGTCTTGAAGCTGTTTCAATCAGCTAGAGTAAAGACAGAAGATTGTTGGTCTGCACTCACTGAAAAGTCTTTTGATTTCTGGAGATTGGCATATGGAAAAGTGCGTAATCTGGAAGAACTTTTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTGTGATCTTGGCAGCAGTGCTAGGTGAAGGCATTTGGCAACTTTGTTCTCAAGTCATCTATAAATTGGGTGGTTTGTTTACTAAAGTTGTTGATTTTTGTGAAAAACACTGGAAGGGTTTCTGTGCACAACTTAAAAGAGCAAAGCTCATTGTGACTGAAAAACTTTGTGTTTTGAAAGGAATTGCACAGCATTGTTTTCAACTACTGCTGGATGCAATACACTCCATGTATTTGAGTTTTAAAAAATGTGCACTCGGTAGAATCCATGGAGATTTGCTCTTCTGGAGAGGGGGTGTGCACAAGATTGTTCAAGATGGCGATGAAATTTGGTTTGACGCCATTGACAGTGTAGATGTTGATGATCTGGGTGTAGTCCAAGAAAAACCTATAGATTTTGAGGTTTGTGATGATGTGACACTTTCAGAAAACCAACCAGGACACATGGTTCAAATCGCTGATGAAGGAAAGAACTATATGTTCTTTCGCTTCAAGAAAGATGATAACATTTATTATACACCAATGTCACAACTTGGTGCTATTAATGTTGTTTGCAAAGCAGGTGGCAAGACTGTCACTTTTGGTGAAATAACTGTGAAGGAGATACCACCACCTGAGGTTGTACCTATTAAGGTCAGCATAGAGTGTTGTGGTGAACCATGGAATACAATCTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAGACAGATCTCACAGTAGAACAACTGCTCTCTGTGATCTATGATAAAATGTGTGAAGATCTCAAGTTGTTCCCAGAGGCTCCAGAACCTCCACCATTTGAGAATGTTGCACTTGTGGATAAAACCGGTAAAGACTTGGATTGCATTAAATCATGCCACCTTGTCTACCGCGATTATGAGAGCGATGAGGACATCGAGGAAGAAGATGCTGAGGAGTGTAATACTGCTTCAGATGATGCTGAGGAGTGTGACACTGCATTAGAGTGTGGAGAAGAGGATGAGGACACAAAAGTGTTGGCTCTTATACAAGACCCAGCAAGTAACAAGTACCCTCTTCCCCTTGATGATGATTATAGTGTCTATAATGGTTGTATTGTCCATAAAGACGCTCTGGATGTTGTAAACCTCCCATCAGGTGAAGAGGCTTATGTTATCAACAATTGCTTTGACGGAGCTTTAAAGCCACTCCCAAAAGCTGTTGTTGATGTTCTTGGAGACTGGGGTGCAGCTGTCGATGCTCAGGAGAAATCTCTGAATCAAGAGGCTGCACATGATAATGTTGTTTCATGTGAAGAGACATTAATTGTTGAGGATGTTGTTGTAAGAACAGCCACTCCTGTTGTAGAACGAGAGCAAGAAGTTGTTGTCTACACGCCTGCTTCTGTTGATACTATTAAAGAAACGTCTGAGGAAGTAGATGAGTTTATCTTAATCCCTGATGTTCCTACTGATGAAGAAGTATCTTCAGCACAAGAAGGTTCTAGTGTTGTTGCAGTTGAAGTTTCGACTGGCGCTAGTCAAGAAGCTTCTCCTATTGTAGATGAAGAAGAAAAATTTGAAGTGCCTACTCAAGTTGCTAAACCACAACGTGAGAAGAAGGCAAAGAAGTTTAAAGTCAAACCAACTACGCACAAGCCTCCTAAGGATTTGGAGTATAAAACATGTGTAGGCGACTTGACTGTTGTAATTGCCAAAGCTTTGGATGAATTTAAAGAGTTCTGCGTTGTAAACGCTGCAAATGAGCATATGTCTCATGGGGGCGGCGTTGCTAAAGCAATTGCAGATTTTTGTGGTCCTGATTTTGTGGAATATTGTGAAGACTATGTTAAGAAACATGGTCCACAGCAAAGACTTGTTACACCTTCTTATGTCAAAGGCATTCAATGTGTTAACAATGTTGTTGGACCACGCCATGGAGATTGCGACTTGTTTAATAAACTTGTCGCTGCCTACAAGAATGTTCTTGTAGATGGTGTGGTTAATTATGTTGTTCCAGTGCTTTCTTCAGGCATTTTTGGAGTTGATTTCAAATTGTCAATAGACGCTATGCGTGAAGCTTTTAATGGCTGCAACATACGTGTCCTGCTCTTTTCTCTGTCGCAAGAACACATCAATTATTTTGATGCAACTTGCAGACAGAAGACAATTTATATTACGGAGGACGGTGTTAAATTCCGTTCTGTTATTGTTAAACCTGGTGATTCTTTGGGTCAATTTGGACAGGTTTTTACGAGACACAAGACAGTCTTTACTGCTGATGATGTAGAGGATAATGAAATTCTCTTCGTTCCAACGACAGATAAGGCTGTGCTTGATTATTATGGTTTAGATGCGCAAAAGTATGTGAAATTTTTACATACACTTGCGCAGAAGTGGAATGTTCTTTATAAGGACAATTTTGTTATACTTGAGTGGCGTGATGGAAATTGCTGGATAAGTGCAGCAATTGTTATCCTTCAAGCCGCTAAGATTAGGTTTAGAGGTTTTCTTGCAGAAGCATGGGCTAAGCTGTTGGGTGGAGACCCCACTGACTTTGTAGCTTGGTGCTATGCAAGTTGCAATGCTAATGTTGGTGAGTTTTCAGATGCTAATTGGCTTTTGGCCAATTTAGCTGAATACTTTGATGCAGATTACACTAATGCACTTCTTAAGAGGCGTGTTATGTGCAACTGTGGTGTTAAAGTTCAGGAGTTTAGGGGCCTTGAAGCTTGTATTCAACCAGTAAAGGCACCTAACCTTCTACATTTTAAGACGCAATATTCAAATTGCCCTGTTTGTGGTTCAAATAGTACAGATGAAGTAGTTGATGCTACACTTCCTTATCTGCTACTAGTAGCTACAGATGGACCTGCAACAGTCGATTGTGATGAAAATGCTGTAGGGAACATTGTATTTATAGGTTCCACCAATAGTGGTCATTGTTATACTCAAGCCGTAGGTAAGGCTTTTGACAATCTTGCTAAGGATAGAAAATATGGAAGGAGGTCGCCTTACATTACAGCAATGTACACGCGTTTCTCCCTTAAGAGTGAAAGTTCTTTACCTGTTGCTACTAAAAAGAGCAAGGGTAAAGGTAAAGTAGTAAAAGAAGATGTTTCTGACCTTGCCACTAGTTCAAAGGCCAGTTTTGATGATCTTACTGACTTTGAAAGGTGGTATGATAGTAACATCTACGAGAGTCTTAAAGTGCAGGATGTACCTGAGAATATTGATGAATATGTGTCATTCACAACAAAGGAAGATTCTAAGTTGCCACTGACACTTAAAGTTAGAGGTATTAAATCAGTTGTCAACTTCAAGTCAAAAGATGGTTTTACGTACAACTTGACACCTGATAATGACGAAAATTCAAAAGCACCAATCTACTACCCAGTCTTAGACTCTGTTAGTCTTAAGGCTATATGGGTAGAAGGCAGTGCTAACTTTGTTGTTGGACATCCAAATTATAATAGTAGAGCGCTGCGTATTCCTACATTTTGGGAATGTGCAGAGAGCTTTGTTAAAATAGGTGAAAAGATTGATGGTGTAACTATGGGCCTATGGCGTGCAGAACACCTTAATAAACCTAATCTAGAAAAGATGTTCAGCGTCATTAAGAAAATGATTGCTGGATCGAGTTTTGTCACCACACAGTGTGGCAAAATGATTGGTAATGCAGCTACATTCGCTGCCGATAAAGTTGGCGGTGGTGTAGTGCGTAATATTACAGATAGAATTAAGGGTGTTTATGGAAGTACACGTGGACATTTTGAAAGAAAAATGTCACTACAATTTCTAAAAACACTCTTTTTCTTTTTCTTTTATCTCTTAAAAGCAGGCGCTAAGAGTTTGGTTGCTAGCTATAAGAGTGTGTTATGTAAAGTAGTCTTTACTACTTTATTTATATTATGGTTCTTATACACAAGTAATCCAGTAATGTTTACTGGTGTACGAGTGTTAGACTTCTTATTTGAAGGCTCATTCTGTAGTCCTTATCAAGATTATGGGAGGGATTCGTTTGATGTTTTACGCTATTGTGCAGAAGATTTTACTTGTCGTGTGTGTTTACATGACAGGGATTCACTTCACTTGTATAAACATGCTTATAGCGTAGAACAGGTTTATAAAGCTGCAGTTTCTGGCATTAGTTTTAATTGGAATTGGCTTTATTTGGTCTTCCTAATATTGTTTGTTAAGCCAGTAGCAGGTTTTGTTATTATTTGCTATTGTGTTAAGTATTTGGTATTGAGTTCAACTGTCCTCCAAACCGGTGTGAGTTTTCTAGATTGGTTTATTCAAACAGTCTTTACTCACTTTAATTTCATGGGTGCAGGATTTTACTTCTGGCTCTTTTATAAGATATACATACAGGTGCATCATATACTGTATTGTAAGGATATAACATGTGAAGTGTGCAAGAGAGTTGCGCGCAGCAACAGACATGAGGTTAGCGTTGTTGTAAGTGGACGCAAACAAATTGTGCATGTGTACACTAATTCTGGCTACAATTTTTGTAAGAGACATAACTGGTATTGTAAGAATTGTGATGAGTATGGTCATCAAAATACATTTATGTCTCCTGAAGTTGCTGGTGAGCTTTCTGAAAAGCTTAAACGCCATGTTAAACCTACAGCTCATGCTTACCACATTGTGGATGAGGCGTGTTTAGTTGAGGATTTTGTTAATCTAAAGTACAAAGCTGCAGTTCCTGGTAAGGATAGTGCAGCTCCTGCAGTTAAATGTTTCAGTGTTACAGACTTTTTGAAAAAAGCTGTATTCCTTAAGGATGCACTGAAATGTGAACAAATCTCTAATGATGGTTTTATAGTGTGTAATACACAGAGTGCGCATGCATTGGAAGAAGCAAAGAATGCTGCTATCTACTATGCGCAATATCTGTGTAAACCAATACTTATACTTGATCAGGCACTTTACGAGCAATTAGTAGTGGAACCTGTGTCTAAGAGTGTTGTAGATAAGGTGTGTAGCATCTTGTCTAATATAATTTCTGTAGATACTGCTGCTTTAAATTATAAGGCGGGTACACTACGTGATGCTCTCCTTTCTATTACTAAAGATGAAGAAGCTGTTGATATGGCTATCTTCTGTCATAATCATGAAGTGGAATACACTGGTGACGGTTTTACTAATATAATACCGTCATATGGTATAGACACTGATAAATTAACACCTCGTGATAGAGGGTTTTTAATAAACGCAGATGCTTCCGTATCTAACCTAAGAGTTAAAAATGCTCCACCGGTGGTATGGAAGTTTTCTGATCTTATTAAGTTGTCTGACAGTTGCCTTAAATATTTAATTTCAGCAACTGTCAAGTCAGGAGGTCGGTTCTTTATTACAAGGTCTGGTGCTAAACAAATTATTTCGTGTCACACACAGAAATTGTTGGTAGAGAAAAAGGCTGGTGGTGTTGTTAGCAGCACTTGTAAAAAGGTTAAGAGTTGTTGTAAATGGCTTTTGATCTTTTATATATTCTTTATAGCATGTTGTTTGGGTTACTATCACATGGAGGGTAGAAGTTTTGATCATCCTATGTATGATGTTAACTCTACACTGCATGTTGAAGGTTTTAAGGTTATAGATAAAGGTGTTATTAGGGACATTGTGCCAGAAGACACCTGCTTTTCTAATAAGTTTGTTAATTTTGATGCCTTTTGGGGTAAACCATATGAAAATAGTAGAGACTGTCCAATTGTAACAGCCATTATAGATGGTGCTGGAACAGTAGCTGCTGGAGTTCCTGGTTTCGTACAGTGGGTAATGGATGGAGTTATGTTTGTACACATGACACAAACTGAGCGAAAACCTTGGTATATTCCTACTTGGTTTAATAGAGAAATTGTTGGTTACACTCAGGACTCAATTATTACTGAGGGTAGTTTTTATACATCTATAGCGTTGTTTTCAGCTAGGTGCTTATATTTAACAGCCAGCAATACACCACAGTTGTATTGTTTTAACGGCGACAATGATGCTCCTGGTGCTTTACCATTTGGAAGTATAGTCCCACATAGGGTCTACTTTCAACCAAATGGGGTTAGACTTATAGTTCCTCAGCAAATACTGCACACACCCTACATAGTAAAGTTTGTATCAGACAGCTATTGTAGAGGTAGTGTATGTGAGTACACTAGGCCAGGTTACTGTGTGTCATTGGACTCTAAATGGGTCTTGTTTAATGATGAGTACACGGTTAAACCAGGTGTGTTTTGTGGTTCTACTGTTAGAGAACTTATGTTTAATATGGTTAATACATTTTTTACTGGTGTTAACCCTAATATTTATATGCAACTGGCAACTATGTTTTTAATATTAGTTGTTGTTGTATTAATTTTTGCAATGGTTATAAAGTTTCAAGGTGTATTTAAAGCATATGCCACCATTGTGTTTATAATAATGTTAGTTTGGGTTATTAATGCATTTATTCTTTGTGTACATAGTTATAATAGTGTTTTAGCTGTTATATTACTTGTACTCTATTGCTATGCATCATTGGTTACAAGCCGCAATACTTCTATAATAATGCATTGCTGGCTTGTGTTTACTTTTGGTTTAATAGTGCCTATGTGGATAGCCTGTTGCTATTTGGCATTTGTACTTTATGTCTATACACCATTTTTCTTTTGGTGTTATGGTACTACTAAAAATATTCGTAAGCTTTATGATGGCAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGCACTTTTGTGATTCGTGGTTCTGAATTTGTTAAGCTTACGAATGAGATAGGTGATAAATTTGATGCCTATCTTTCTGCGTACGCTAGACTTAAATATTACTCAGGCACTGGAAGTGAACAGGACTACATTCAAGCCTGTCGTGCTTGGTTGGCTTATGCACTTGACCAATATAGAAATAGTGGTGTAGAAATGGTTTATACTCCACCTCGTTACTCTATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAGCTAGTTTCTCCTAGTAGTGCTGTTGAAAGGTGCATTGTTAGTGTGTCTTATAGGGGCAACAACCTTAATGGGCTTTGGTTAGGTGATACTATCTATTGCCCACGGCATGTGTTAGGTAAGTATTCAGGTGACCAATGGACTGATGTTCTTAACCTTGCTAATAACTATGAGTTTGAAGTTACGACTCAAAATGGAAGCACTTTGAATGTTGTCAGTAGGCGTTTGAAAGGTGCAGTTTTAATATTGCAAACTGCTGTTGCTAATGCTGAAACTCCAAAGTATAAATTTTTGAAGGCAAATTGTGGTGATAGTTTCACTATAGCTTGTTCTTATGGTGGTACAGTTATTGGACTTTACCCTGTTACTATGCGTTCTAATGGTACTATTAGAGCATCTTTCCTTGCTGGAGCCTGTGGTTCAGTAGGTTTTACTATAGAAAAGGGTGTAGTTAATTTCTTTTATATGCACCATCTTGAGCTACCTAATGCATTACACACTGGAACTGACCTAATGGGTGAATTCTATGGTGGTTATGTGGATGAAGAGGTTGCACAAAGAGTGCCACCAGATAACTTAGTTACTAACAATATTGTTGCATGGCTTTATGCTGCAATTATTAGTGTTAAAGAGAGTAGTTTTTCAAACCCTAAATGGATTGAGAGTACTACTGTTAGTGTTGATGATTATAATAGGTGGGCTGGTGATAATGGTTTTACACCATTTTCTACTAGTACTGCTATTACTAAATTAAGTGCTATTACGGGAGTTGATGTTTGTAAGCTCCTTCGCACTATTATGGTGAAAAACGGTCAGTGGGGTGATGAACCCATTTTAGGCCAATACAATTTTGAAGATGAGCTAACACCAGAATCTGTTTTTAATCAGGTTGGTGGTGTAAGGTTACAATCTTCTTTTGTGAAAAAAGCTACATCTTGGTTTTGGAGTAGATGTGTTTTAGCTTGTTTCTTGTTTGTGTTGTGTGCTATTGTCTTATTTACGGCAGTGCCACTTAGGTTTTATGTACATGCGGCTGTTATTTTGTTATCAGCTGTTCTGTTTATTTCTTTTACTGTGAAACATGTTATGGCATATATGGATACTTTCTTATTGCCGACATTGCTTACAGTTATTATTGGAGTTTGTGCTGAAGTACCTTTCATCTACAATACTCTAATTAGTCAAGTTGTTATATTCTTTAGTCAATGGTATGACCCAGTAATGTTTGATACTATGGTACCATGGATGTTCTTACCATTAGTCTTGTATACTGCTTTTAAGTGTGTACAGGGCTGTTATAGTGTGAATTCCTTTAGTACTTCTCTGTTAGTGTTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTATGCCTCTTCTAATACGCTGGCAGCATACTCAGAGGGTAATTGGGAATTATTCTTTGAATTAGTTCATACTACTGTTTTGGCTAATATTAGTAGCAACTCTTTAGTTGGTTTAATTGTGTTTAAGTTTGCTAAATGGATGCTGTGTTATTGTAATGCAACATACTTTAATAATTATGTGCTAATGGCGGTCATTGTTAATGTCCTAGGTTGGCTTTTTACTTGTTACTTTGGAATATATTGGTGGGTTAATAAGGTTTTTGGTTTAACTTTAGGCAAATATAACTTCAAAGTTTCTGTGGACCAATATAGGTATATGTGTCTTCATAAGATAGCACCACCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGAATAGGTGGTGATCGTGTGTTACCTATTGCTACAGTTCAATCTAAGTTGAGTGATGTAAAATGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCACGCTTATCTTGTTGATTTACACAATAAAATTCTTGCCTCTGACGATGTCGGAGAGTGTATGGATAATTTATTGGGTATGCTTATTACGCTATTTTGTATTGATAGTACTATTGATTTAAGTGAGTATTGTGATGACATACTTAAGAGGTCAACTGTTTTACAATCAGTTACTCAAGAGTTTTCACATATACCTTCTTACGCAGAGTATGAAAGAGCTAAACAACTTTACGAGAAAGTGTTAGCTGATTCTAAAAATGGTGGTGTGACACAGCAAGAGCTTGCTGCATATAGAAAAGCTGCCAATATTGCAAAGTCTGTCTTTGATAGAGACTTGGCTGTTCAGAAAAAGTTAGATAGCATGGCAGAACGTGCTATGACAACAATGTATAAAGAAGCGCGTGTTACTGATAGGAGGGCAAAGTTGGTCTCATCTCTACATGCGCTACTCTTTTCAATGCTTAAGAGGCTTGATTCTGAAAAGCTTAATGTATTATTTGATCAGGCGAGTAGTGGTATAGTACCACTTGCGACTGTACCAATAGTTTGTAGTAATAAGCTTACACTTGTGATACCAGACCCAGATACGTGGGTTAAATGTGTTGAAGGTATGCATGTTACTTATTCAACAGTAGTCTGGAATATAGACAATGTTATTGATGCTGATGGCACAGAATTACATCCATCTTCTACAGGTAATGGTTTGTCCTACTGTATAAGTGGTAATAATATAGCATGGCCTCTAAAGGTCAACTTGACTAGGAATGGGCATAATAAAGTTGATGCTGCATTGCAGAATAACGAGCTTATGCCCCATGGTGTAAAAACAAAGGCTTGCGTTGCAGGTGTTGATCAAGCACACTGTAGCGTAGAGTCTAAATGTTATTATACTAATATTAGTGGTAATTCAGTTGTAGCTGCAATTACTTCTTCAAACCCAAATTTGAAAGTTGCTTCATTTTTAAATGAGGCAGGTAATCAAATATATGTAGACTTAGACCCACCATGTAAATTTGGTATGAAGGTTGGTGATAAGGTAGAAGTTGTTTACCTGTATTTTATTAAAAATACAAGGTCCATTATTAGAGGTATGGTACTTGGGGCTATATCTAATGTTGTTGTCTTACAGTCTAAAGGACATGAAACAGAGGAAGTATATGCCGTTGGCATTCTTTCTCTATGTTCTTTTGCAGTAGACCCAGCTGATACATATTGTAAATATGTAGCTGCTGGTAATCAACCTTTAGGCAACTGTGTTAAAATGTTGACAGTGCATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGTCCAACTCCTGATCAGGATTCTTATGGAGGAGCGTCTGTGTGTCTCTATTGTAGGGCACACATAGCACATCCAGGAGGAGCAGGAAACTTGGATGGACGTTGTCTGTTCAAAGGTTCTTTTGTGCAAATACCCACTACGGAGAAAGATCCAGTAGGGTTCTGTCTGCGTAATAAGGTGTGCACTGTCTGTCAATGTTGGGTTGGCTATGGGTGTCAATGCGACTCACTTAGACAACCTAAATCTAATGTGCAATCGGACGCTGGTGCGCCTGGTTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGA
Protein
MASSLKQGVSSQNQRSVILVAKDIPDQLRDALFFYTSHNPKDYADAFAVRQKFDRNLGAGKQFKFETVCGPFFLKGVDKITPGVPAKSLKATAKLADIEGIFGVSPLARKYRELLKTASQWSFNVETLDACAQTLDEIFEPTEILWLQVASKIQVAAMAMRRLVGEVTAKVMEALGSSLGSLFQIVKGTMSRIFQKALAIFDNVSELPQRILALKMAFAKCAKSITVVVVERALVIREFAGTCLASINGAVAKFFEELPNGFMGAKVFSTLAFFKEAAVKIVENIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEIPVEPEGWSAILEGHLCYVFKSGDRFYATPLSGNFALHDVYCCERVVCLTDGVTPEINDGLILAAIYSSFSISELVAALKKGEPFKFLGHKFVYVKEAAVSFTLGKAATIADVLKLFQSARVKTEDCWSALTEKSFDFWRLAYGKVRNLEELLKTHFCKAQMSIVILAAVLGEGIWQLCSQVIYKLGGLFTKVVDFCEKHWKGFCAQLKRAKLIVTEKLCVLKGIAQHCFQLLLDAIHSMYLSFKKCALGRIHGDLLFWRGGVHKIVQDGDEIWFDAIDSVDVDDLGVVQEKPIDFEVCDDVTLSENQPGHMVQIADEGKNYMFFRFKKDDNIYYTPMSQLGAINVVCKAGGKTVTFGEITVKEIPPPEVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYDKMCEDLKLFPEAPEPPPFENVALVDKTGKDLDCIKSCHLVYRDYESDEDIEEEDAEECNTASDDAEECDTALECGEEDEDTKVLALIQDPASNKYPLPLDDDYSVYNGCIVHKDALDVVNLPSGEEAYVINNCFDGALKPLPKAVVDVLGDWGAAVDAQEKSLNQEAAHDNVVSCEETLIVEDVVVRTATPVVEREQEVVVYTPASVDTIKETSEEVDEFILIPDVPTDEEVSSAQEGSSVVAVEVSTGASQEASPIVDEEEKFEVPTQVAKPQREKKAKKFKVKPTTHKPPKDLEYKTCVGDLTVVIAKALDEFKEFCVVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSYVKGIQCVNNVVGPRHGDCDLFNKLVAAYKNVLVDGVVNYVVPVLSSGIFGVDFKLSIDAMREAFNGCNIRVLLFSLSQEHINYFDATCRQKTIYITEDGVKFRSVIVKPGDSLGQFGQVFTRHKTVFTADDVEDNEILFVPTTDKAVLDYYGLDAQKYVKFLHTLAQKWNVLYKDNFVILEWRDGNCWISAAIVILQAAKIRFRGFLAEAWAKLLGGDPTDFVAWCYASCNANVGEFSDANWLLANLAEYFDADYTNALLKRRVMCNCGVKVQEFRGLEACIQPVKAPNLLHFKTQYSNCPVCGSNSTDEVVDATLPYLLLVATDGPATVDCDENAVGNIVFIGSTNSGHCYTQAVGKAFDNLAKDRKYGRRSPYITAMYTRFSLKSESSLPVATKKSKGKGKVVKEDVSDLATSSKASFDDLTDFERWYDSNIYESLKVQDVPENIDEYVSFTTKEDSKLPLTLKVRGIKSVVNFKSKDGFTYNLTPDNDENSKAPIYYPVLDSVSLKAIWVEGSANFVVGHPNYNSRALRIPTFWECAESFVKIGEKIDGVTMGLWRAEHLNKPNLEKMFSVIKKMIAGSSFVTTQCGKMIGNAATFAADKVGGGVVRNITDRIKGVYGSTRGHFERKMSLQFLKTLFFFFFYLLKAGAKSLVASYKSVLCKVVFTTLFILWFLYTSNPVMFTGVRVLDFLFEGSFCSPYQDYGRDSFDVLRYCAEDFTCRVCLHDRDSLHLYKHAYSVEQVYKAAVSGISFNWNWLYLVFLILFVKPVAGFVIICYCVKYLVLSSTVLQTGVSFLDWFIQTVFTHFNFMGAGFYFWLFYKIYIQVHHILYCKDITCEVCKRVARSNRHEVSVVVSGRKQIVHVYTNSGYNFCKRHNWYCKNCDEYGHQNTFMSPEVAGELSEKLKRHVKPTAHAYHIVDEACLVEDFVNLKYKAAVPGKDSAAPAVKCFSVTDFLKKAVFLKDALKCEQISNDGFIVCNTQSAHALEEAKNAAIYYAQYLCKPILILDQALYEQLVVEPVSKSVVDKVCSILSNIISVDTAALNYKAGTLRDALLSITKDEEAVDMAIFCHNHEVEYTGDGFTNIIPSYGIDTDKLTPRDRGFLINADASVSNLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGGRFFITRSGAKQIISCHTQKLLVEKKAGGVVSSTCKKVKSCCKWLLIFYIFFIACCLGYYHMEGRSFDHPMYDVNSTLHVEGFKVIDKGVIRDIVPEDTCFSNKFVNFDAFWGKPYENSRDCPIVTAIIDGAGTVAAGVPGFVQWVMDGVMFVHMTQTERKPWYIPTWFNREIVGYTQDSIITEGSFYTSIALFSARCLYLTASNTPQLYCFNGDNDAPGALPFGSIVPHRVYFQPNGVRLIVPQQILHTPYIVKFVSDSYCRGSVCEYTRPGYCVSLDSKWVLFNDEYTVKPGVFCGSTVRELMFNMVNTFFTGVNPNIYMQLATMFLILVVVVLIFAMVIKFQGVFKAYATIVFIIMLVWVINAFILCVHSYNSVLAVILLVLYCYASLVTSRNTSIIMHCWLVFTFGLIVPMWIACCYLAFVLYVYTPFFFWCYGTTKNIRKLYDGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFDAYLSAYARLKYYSGTGSEQDYIQACRAWLAYALDQYRNSGVEMVYTPPRYSIGVSRLQAGFKKLVSPSSAVERCIVSVSYRGNNLNGLWLGDTIYCPRHVLGKYSGDQWTDVLNLANNYEFEVTTQNGSTLNVVSRRLKGAVLILQTAVANAETPKYKFLKANCGDSFTIACSYGGTVIGLYPVTMRSNGTIRASFLAGACGSVGFTIEKGVVNFFYMHHLELPNALHTGTDLMGEFYGGYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSNPKWIESTTVSVDDYNRWAGDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMVKNGQWGDEPILGQYNFEDELTPESVFNQVGGVRLQSSFVKKATSWFWSRCVLACFLFVLCAIVLFTAVPLRFYVHAAVILLSAVLFISFTVKHVMAYMDTFLLPTLLTVIIGVCAEVPFIYNTLISQVVIFFSQWYDPVMFDTMVPWMFLPLVLYTAFKCVQGCYSVNSFSTSLLVLYQFMKLGFVIYASSNTLAAYSEGNWELFFELVHTTVLANISSNSLVGLIVFKFAKWMLCYCNATYFNNYVLMAVIVNVLGWLFTCYFGIYWWVNKVFGLTLGKYNFKVSVDQYRYMCLHKIAPPKTVWEVFSTNILIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVDLHNKILASDDVGECMDNLLGMLITLFCIDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKQLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKRLDSEKLNVLFDQASSGIVPLATVPIVCSNKLTLVIPDPDTWVKCVEGMHVTYSTVVWNIDNVIDADGTELHPSSTGNGLSYCISGNNIAWPLKVNLTRNGHNKVDAALQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIIRGMVLGAISNVVVLQSKGHETEEVYAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCLFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWVGYGCQCDSLRQPKSNVQSDAGAPGFDKNYLNGYGVAVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Catalytic Activity
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H2O = ADP + H(+) + phosphate
ATP + H2O = ADP + H(+) + phosphate
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity). Interacts with DDX1 (via C-terminus).
Miscellaneous
Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
ATP-binding
Endonuclease
Exonuclease
Helicase
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Methyltransferase
Nuclease
Nucleotide-binding
Nucleotidyltransferase
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
RNA-directed RNA polymerase
Thiol protease
Transferase
Transmembrane
Transmembrane helix
Viral RNA replication
Zinc
Zinc-finger
Feature
chain Non-structural protein 2
Uniprot
Pubmed
Proteomes
PRIDE
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR029063 SAM-dependent_MTases
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR037227 EndoU-like
IPR027417 P-loop_NTPase
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR013016 Peptidase_C30/C16
IPR003593 AAA+_ATPase
IPR040795 Nsp1a_N
IPR001205 RNA-dir_pol_C
IPR027352 CV_ZBD
IPR014828 NSP7
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR009469 RNA_pol_N_coronovir
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR007094 RNA-dir_pol_PSvirus
IPR027351 (+)RNA_virus_helicase_core_dom
IPR036499 NSP9_sf
IPR014829 NSP8
IPR042515 Nsp15_N
IPR009461 Coronavirus_NSP16
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR009466 NSP11
IPR029063 SAM-dependent_MTases
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR037227 EndoU-like
IPR027417 P-loop_NTPase
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR013016 Peptidase_C30/C16
IPR003593 AAA+_ATPase
IPR040795 Nsp1a_N
IPR001205 RNA-dir_pol_C
IPR027352 CV_ZBD
IPR014828 NSP7
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR009469 RNA_pol_N_coronovir
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR007094 RNA-dir_pol_PSvirus
IPR027351 (+)RNA_virus_helicase_core_dom
IPR036499 NSP9_sf
IPR014829 NSP8
IPR042515 Nsp15_N
IPR009461 Coronavirus_NSP16
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR009466 NSP11
SUPFAM
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 642 Identity=87.15%
Cov(Q)=9.02% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0008168 F:methyltransferase activity
GO:0004519 F:endonuclease activity
GO:0039520 P:induction by virus of host autophagy
GO:0003724 F:RNA helicase activity
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0006351 P:transcription, DNA-templated
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0033644 C:host cell membrane
GO:0039694 P:viral RNA genome replication
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0005524 F:ATP binding
GO:0016021 C:integral component of membrane
GO:0016896 F:exoribonuclease activity, producing 5'-phosphomonoesters
GO:0004519 F:endonuclease activity
GO:0039520 P:induction by virus of host autophagy
GO:0003724 F:RNA helicase activity
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0006351 P:transcription, DNA-templated
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0033644 C:host cell membrane
GO:0039694 P:viral RNA genome replication
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0005524 F:ATP binding
GO:0016021 C:integral component of membrane
GO:0016896 F:exoribonuclease activity, producing 5'-phosphomonoesters
Subcellular Location
From MSLVP
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 5 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 5 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 5 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 5 publications.
Topology
Length:
3969
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
419.285779999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00278
outside
1 - 1742
TMhelix
1743 - 1762
inside
1763 - 1768
TMhelix
1769 - 1791
outside
1792 - 1847
TMhelix
1848 - 1870
inside
1871 - 1876
TMhelix
1877 - 1899
outside
1900 - 1903
TMhelix
1904 - 1926
inside
1927 - 2298
TMhelix
2299 - 2317
outside
2318 - 2378
TMhelix
2379 - 2401
inside
2402 - 2574
TMhelix
2575 - 2597
outside
2598 - 2601
TMhelix
2602 - 2624
inside
2625 - 2630
TMhelix
2631 - 2648
outside
2649 - 2667
TMhelix
2668 - 2690
inside
2691 - 3112
TMhelix
3113 - 3135
outside
3136 - 3139
TMhelix
3140 - 3159
inside
3160 - 3170
TMhelix
3171 - 3193
outside
3194 - 3212
TMhelix
3213 - 3235
inside
3236 - 3241
TMhelix
3242 - 3264
outside
3265 - 3273
TMhelix
3274 - 3296
inside
3297 - 3315
TMhelix
3316 - 3338
outside
3339 - 3969
