Strain
Porcine_NC_003436
(Region: Belgium; Strain: Porcine epidemic diarrhea virus, complete genome.; Date: 1977)
Gene
Pol1
Description
Annotated in NCBI,
Pol1
Location
GenBank Accession
Full name
Replicase polyprotein 1ab
Alternative Name
ORF1ab polyprotein
Sequence
CDS
ATGGCTAGCAACCATGTCACATTGGCGTTTGCCAATGATGCAGAAATTTCGGCTTTTGGCTTTTGCACTGCTAGTGAAGCCGTCTCATACTATTCTGAGGCCGCCGCTAGTGGATTTATGCAATGCCGTTTTGTGTCCCTCGATCTCGCTGACACTGTTGAGGGATTGCTTCCTGAAGACTATGTCATGGTGGTGATCGGCACTACCAAGCTTAGTGCGTATGTGGACACTTTTGGTAGCCGCCCCAGAAACATTTGTGGTTGGCTGTTATTTTCTAACTGTAATTACTTCCTCGAAGAGTTAGAGCTCACTTTTGGTCGTCGTGGTGGTAACATCGTGCCAGTTGACCAATACATGTGTGGCGCTGACGGGAAACCTGTTCTTCAGGAATCCGAGTGGGAGTATACAGATTTCTTCGCTGACTCCGAAGACGGTCAACTCAACATTGCTGGGATCACTTATGTGAAGGCCTGGATTGTAGAGCGATCGGACGTCTCTTATGCGAGTCAGAATTTAACATCTATTAAGTCTATTACTTACTGTTCAACCTATGAGCATACTTTTCTTGATGGTACTGCCATGAAGGTTGCACGTACTCCAAAGATCAAGAAGAATGTTGTTTTGTCTGAGCCACTTGCTACTATCTACAGGGAAATTGGTTCCCCTTTTGTGGATAATGGGAGCGATGCTCGTTCTATCATTAGGAGACCAGTGTTCCTCCACGCTTTTGTTAAGTGTAAGTGTGGTAGTTATCATTGGACTGTTGGTGATTGGACTTCCTATGTCTCCACTTGCTGTGGCTTTAAGTGCAAGCCAGTCCTTGTGGCCTCATGCTCTGCTATGCCTGGTTCTGTTGTGGTCACACGCGCTGGTGCTGGCACTGGTGTTAAGTACTACAACAACATGTTCCTGCGCCATGTGGCTGACATTGATGGATTGGCATTCTGGCGAATTCTTAAGGTGCAGTCCAAAGACGACCTCGCTTGCTCTGGTAAGTTCCTTGAACACCATGAGGAAGGTTTCACAGACCCTTGCTACTTTTTGAATGATTCGAGCCTTGCTACTAAGCTTAAGTTCGACATCCTTAGTGGCAAGTTTTCTGATGAAGTCAAACAAGCTATCATTGCTGGTCATGTTGTTGTTGGCAGTGCACTCGTTGACATTGTTGACGATGCACTGGGACAACCTTGGTTCATACGTAAGCTTGGTGACCTTGCAAGTGCACCTTGGGAGCAGCTTAAGGCTGTCGTTAGAGGCCTTGGCCTCTTGTCTGATGAGGTTGTGCTCTTTGGCAAAAGACTTAGCTGTGCCACTCTTAGTATCGTTAACGGCGTTTTTGAGTTTCTCGCCGATGTGCCAGAGAAGTTGGCTGCGGCTGTTACAGTTTTTGTCAACTTCTTGAATGAGTTCTTTGAGTCTGCCTGTGACTGCTTAAAGGTCGGAGGTAAGACATTCAACAAGGTTGGTTCCTATGTTCTTTTTGACAATGCATTGGTTAAGCTTGTCAAGGCAAAAGCTCGCGGCCCACGACAGGCAGGTATTTGTGAAGTTCGCTACACAAGCCTGGTTGTTGGGAGTACTACCAAGGTGGTTTCCAAGCGCGTTGAAAATGCCAATGTGAATCTCGTCGTCGTTGACGAGGATGTAACCCTCAACACCACTGGTCGTACAGTTGTCGTTGATGGACTTGCATTCTTCGAGAGTGACGGGTTTTACAGACATCTTGCTGATGCTGACGTTGTCATCGAACATCCTGTTTATAAGTCTGCTTGTGAGCTCAAGCCAGTTTTTGAGTGTGACCCAATACCTGATTTTCCTTTGCCTGTGGCCGCTAGTGTTGCAGAGCTCTGTGTGCAAACCGATCTGTTGCTTAAAAATTACAACACTCCTTATAAAACTTACAGCTGCGTAGTGAGAGGTGATAAGTGTTGCATCACTTGCACCTTACAGTTCAAAGCACCAAGTTATGTGGAGGATGCTGTTAATTTTGTAGACCTATGTACCAAGAACATTGGTACTGCTGGTTTTCATGAGTTTTACATAACGGCCCATGAACAACAGGATCTGCAAGGGTTTTTAACCACTTGTTGCACGATGTCAGGTTTTGAGTGTTTTATGCCTACAATCCCACAGTGTCCAGCAGTGCTTGAAGAGATTGATGGTGGTAGCATATGGCGGTCTTTTATCACTGGTCTTAATACAATGTGGGATTTTTGCAAGCGTCTTAAAGTCAGCTTTGGACTGGATGGCATTGTTGTCACTGTAGCACGCAAATTTAAACGACTTGGTGCTCTTTTGGCAGAAATGTATAACACTTATCTTTCAACTGTGGTGGAAAACTTGGTACTGGCCGGTGTTAGCTTCAAGTATTATGCCACCAGTGTCCCAAAAATTGTTTTGGGCGGTTGTTTTCACAGTGTTAAAAGTGTTTTTGCAAGTGTCTTCCAGATTCCTGTCCAGGCAGGCATTGAGAAGTTTAAAGTTTTCCTTAACTGTGTTCACCCTGTTGTACCACGTGTCATTGAAACTTCTTTTGTGGAATTAGAAGAGACGACATTTAAACCACCAGCACTCAATGGTGGTATTGCTATTGTTGATGGCTTCGCTTTCTATTATGATGGAACACTATACTATCCCACCGATGGTAATAGTGTTGTGCCTATCTGTTTTAAGAAGAAAGGTGGTGGTGATGTTAAATTCTCTGATGAAGTCTCTGTTAAAACCATTGATCCAGTTTATAAGGTCTCCCTTGAATTTGAGTTCGAGTCTGAGACTATTATGGCTGTGCTTAATAAGGCTGTTGGTAATCGTATCAAGGTTACAGGTGGTTGGGATGATGTCGTTGAGTATATTAACGTTGCCATTGAGGTTCTTAAAGATCACGTTGAAGTGCCTAAGTACTACATCTATGATGAGGAAGGTGGCACTGATCCTAATCTGCCCGTAATGGTTTCTCAGTGGCCGTTGAATGATGACACGATCTCACAGGACCTGCTTGATGTGGAAGTTGTTACGGATGCACCAATTGATTCCGAGGGTGATGAAGTAGACTCCTCTGCACCTGAGAAGGTGGCAGATGTGGCTAACTCTGAACCTGGGGATGATGGTCTTCCTGTGGCACCTGAAACAAATGTAGAGTCTGAAGTTGAGGAAGTTGCCGCAACCTTGTCCTTTATTAAAGATACACCTTCCACAGTTACTAAGGACCCTTTTGCTTTTGACTTTGTAAGCTACGGAGGACTTAAAGTTTTAAGACAATCTCATAACAATTGTTGGGTTACTTCTACCTTGGTGCAGCTACAATTGCTTGGCATCGTTGATGACCCTGCAATGGAACTTTTTAGTGCTGGTAGAGTTGGTCCAATGGTTCGCAAATGTTATGAGTCACAAAAGGCTATTTTGGGATCTTTGGGTGATGTGTCGGCTTGCCTAGAGTCTCTGACTAAGGACCTACACACACTTAAGATTACCTGTTCTGTAGTCTGCGGTTGTGGTACTGGTGAACGTATCTATGAGGGTTGTGCTTTTCGTATGACGCCAACTTTGGAACCGTTTCCATATGGTGCTTGTGCTCAGTGTGCTCAAGTTTTGATGCACACTTTTAAAAGTATTGTTGGCACCGGCATCTTTTGCCGAGATACTACTGCTCTCTCCTTGGATTCTTTGGTTGTAAAACCTCTTTGTGCGGCTGCTTTTATAGGCAAGGATAGTGGTCATTATGTCACCAATTTTTATGATGCTGCTATGGCTATTGATGGTTATGGTCGTCATCAGATAAAGTATGACACACTGAACACCATTTGTGTTAAAGACGTGAATTGGACAGCACCTCTTGTCCCTGCCGTTGATTCTGTAGTGGAGCCTGTTGTCAAACCATTCTATTCTTATAAGAATGTTGATTTTTACCAAGGAGATTTTAGTGACCTTGTTAAACTTCCATGTGACTTTGTTGTTAATGCTGCAAATGAGAAGTTGTCTCATGGTGGCGGCATAGCAAAGGCCATTGATGTTTATACCAAGGGCATGTTGCAGAAGTGTTCAAATGATTACATTAAAGCACACGGTCCCATTAAAGTTGGACGTGGTGTCATGTTGGAGGCATTAGGTCTTAAGGTCTTTAATGTTGTTGGTCCACGTAAGGGTAAGCATGCACCTGAGCTTCTTGTTAAGGCTTATAAGTCCGTTTTTGCTAACTCAGGTGTTGCTCTTACACCTTTGATTAGTGTTGGAATTTTTAGTGTTCCTTTGGAAGAATCTTTATCTGCTTTTCTTGCATGTGTTGGTGATCGCCACTGTAAGTGCTTTTGTTATGGTGACAAAGAGCGCGAGGCGATCATTAAGTACATGGATGGCTTGGTAGATGCTATTTTCAAAGAGGCGCTTGTTGACACTACTCCTGTCCAGGAAGATGTTCAACAAGTTTCACAAAAACCAGTTTTGCCTAATTTTGAACCTTTCAGGATTGAAGGTGCTCATGCTTTCTATGAGTGCAACCCTGAAGGTTTGATGTCCTTAGGTGCTGACAAGCTGGTGTTGTTTACAAATTCCAATTTGGATTTTTGTAGTGTTGGTAAGTGTCTTAACGATGTGACCAGCGGTGCATTGCTTGAAGCCATAAATGTATTTAAAAAGAGTAACAAAACAGTGCCTGCTGGCAACTGTGTTACTTTAGATTGTGCAAATATGATTTCTATTACTATGGTAGTATTGCCATTTGATGGTGATGCTAATTATGACAAAAATTATGCACGCGCCGTTGTCAAGGTATCTAAGCTTAAAGGCAAGTTAGTGCTTGCTGTTGATGATGCCACGTTGTATTCCAAGTTGTCCCACCTCAGCGTGTTAGGTTTCGTGTCCACACCTGACGATGTGGAGCGTTTCTACGCAAATAAGAGTGTGGTTATTAAAGTCACTGAGGATACACGTAGTGTCAAGGCTGTTAAAGTAGAATCCACTGCTACTTATGGACAACAAATTGGACCTTGTCTTGTTAATGACACCGTTGTCACAGACAACAAACCTGTTGTCGCTGACGTTGTAGCTAAGGTTGTACCAAATGCTAATTGGGATTCACATTATGGTTTTGATAAGGCTGGTGAGTTCCACATGCTAGACCATACTGGGTTTACCTTTCCTAGCGAAGTTGTTAACGGTAGGCGTGTGATTAAAACCACAGATAATAATTGTTGGGTTAATGTCACATGTTTACAATTACAGTTTGCTAGATTTAGGTTCAAGTCAGCAGGTCTACAGGCTATGTGGGAGTCCTATTGTACTGGTGATGTTGCTATGTTTGTGCATTGGTTGTACTGGCTTACTGGTGTTGACAAAGGTCAGCCTAGTGATTCAGAAAATGCACTTAACATGTTGTCCAAGTACATTGTTCCTGCTGGTTCTGTCACTATTGAACGTGTCACGCATGACGGCTGTTGTTGTAGTAAGCGTGTTGTTACTGCACCAGTTGTGAATGCTAGTGTGTTGAAGCTTGGCGTCGAGGATGGCCTTTGTCCACATGGTCTTAACTACATTGGCAAAGTTGTTGTAGTTAAAGGTACTACAATTGTTGTCAATGTAGGAAAACCTGTAGTGGCACCATCACACCTCTTTCTTAAGGGTGTATCCTACACAACATTCCTAGATAATGGTAACGGTGTTGTCGGCCATTATACTGTTTTTGATCATGGCACTGGTATGGTGCATGATGGCGATGCTTTTGTACCGGGTGATCTTAATGTATCTCCTGTTACAAATGTTGTTGTCTCAGAGCAGACGGCTGTTGTGATTAAAGACCCTGTGAAGAAAGCAGAGTTAGACGCTACTAAGCTGCTCGACACTATGAATTATGCATCGGAAAGATTTTTTTCCTTTGGTGATTTCATGTCACGTAATTTAATTACAGTGTTTTTGTACATCCTTAGCATTTTGGGTCTCTGTTTTAGAGCCTTTCGTAAGAGAGATGTTAAAGTTCTAGCTGGTGTACCCCAACGTACTGGTATTATATTGCGTAAAAGTATGCGCTATAATGCAAAGGCGTTGGGTGTCTTTTTCAAGCTAAAGCTTTATTGGTTCAAAGTTCTTGGTAAGTTTAGTCTGGGTATTTATGCATTGTATGCATTACTATTCATGACAATACGCTTTACACCTATAGGTAGCCCCGTCTGTGATGATGTTGTTGCTGGTTATGCTAATTCTAGTTTTGACAAGAATGAGTACTGCAACAGTGTTATTTGTAAGGTCTGTCTCTATGGGTACCAGGAACTCTCGGACTTCTCCCACACACAGGTTGTATGGCAACACCTTAGAGACCCATTAATTGGTAATGTGATGCCTTTCTTTTATTTGGCATTTTTGGCAATTTTTGGGGGTGTCTATGTAAAGGCTATTACTCTCTATTTTATTTTCCAGTACCTTAATAGTCTTGGTGTGTTTTTGGGCCTACAGCAGTCCATTTGGTTTTTGCAGCTCGTGCCTTTTGATGTCTTTGGTGATGAGATCGTCGTCTTTTTCATCGTTACACGCGTATTGATGTTCATTAAGCATGTTTGCCTTGGCTGCGATAAGGCATCTTGTGTGGCTTGCTCTAAGAGTGCTCGCCTTAAGCGCGTTCCTGTCCAGACTATCTTTCAGGGTACTAGTAAATCCTTCTACGTACATGCCAATGGTGGTTCTAAGTTCTGTAAGAAGCACAATTTCTTTTGTTTAAATTGTGATTCTTATGGTCCAGGCTGCACTTTTATTAACGACGTCATTGCAACTGAAGTTGGTAATGTTGTCAAACTTAATGTGCAACCGACAGGTCCTGCCACTATTCTTATTGACAAGGTTGAATTCAGTAATGGTTTCTACTATCTTTATAGTGGTGACACATTTTGGAAGTACAACTTTGACATAACAGATAGCAAATACACTTGCAAAGAGGCACTTAAAAATTGTAGCATAATCACAGACTTTATTGTTTTTAACAATAATGGTTCCAATGTAAATCAGGTTAAGAATGCATGTGTGTATTTTTCACAGATGCTTTGTAAACCTGTTAAGTTAGTGGACTCAGCGTTGTTGGCCAGTTTGTCTGTTGATTTTGGTGCAAGCTTACATAGTGCTTTTGTTAGTGTGTTGTCGAATAGTTTCGGCAAAGACCTGTCAAGTTGTAATGACATGCAGGATTGCAAGAGCACATTGGGTTTTGATGATGTACCATTGGATACCTTTAATGCTGCTGTTGCTGAGGCTCATCGCTATGATGTCCTCTTGACTGACATGTCATTCAACAATTTTACCACCAGTTATGCAAAACCAGAGGAAAAATTTCCCGTCCATGACATTGCCACGTGTATGCGTGTAGGTGCCAAGATTGTTAATCATAACGTTCTTGTCAAGGATAGTATACCTGTGGTGTGGCTTGTACGTGATTTCATTGCCCTTTCGGAAGAAACTAGGAAGTACATTATTCGTACGACTAAAGTTAAGGGTATAACATTTATGTTGACCTTTAATGATTGTCGTATGCATACTACCATACCTACTGTTTGCATTGCAAATAAGAAGGGTGCAGGTCTTCCTAGTTTTTCAAAGGTTAAGAAATTCTTTTGGTTTTTGTGTCTGTTCATAGTTGCTGCTTTCTTTGCACTAAGCTTTCTTGATTTTAGTACTCAGGTTAGCAGTGATAGCGATTATGATTTCAAGTATATTGAGAGTGGCCAGTTGAAGACTTTTGACAATCCACTTAGTTGTGTGCATAATGTCTTTATTAACTTCGACCAGTGGCATGATGCCAAGTTTGGTTTCACCCCCGTCAACAATCCTAGTTGTCCTATAGTTGTTGGTGTATCAGACGAAGCTCGCACTGTTCCAGGTATCCCAGCAGGTGTTTATTTAGCTGGTAAAACACTTGTGTTTGCTATTAACACCATTTTTGGTACATCTGGTTTGTGCTTCGATGCTAGTGGCGTTGCTGATAAGGGCGCTTGCATTTTTAATTCAGCTTGCACCACATTATCTGGTTTGGGTGGAACTGCTGTCTACTGTTACAAGAATGGGCTAGTTGAAGGTGCCAAACTTTATAGTGAGTTGGCACCTCATAGCTACTATAAAATGGTAGATGGTAATGCTGTGTCTTTACCTGAAATTATTTCACGCGGCTTTGGCATCCGTACTATCCGTACAAAGGCTATGACTTATTGCCGTGTTGGCCAGTGTGTGCAGTCTGCAGAAGGCGTTTGTTTTGGCGCCGATAGATTCTTTGTCTATAATGCAGAATCTGGTTCTGACTTTGTTTGTGGCACAGGGCTCTTCACATTGTTGATGAACGTTATTAGTGTTTTTTCCAAGACAGTACCAGTAACTGTGTTGTCTGGTCAAATACTTTTTAATTGCATTATTGCTTTTGTTGCTGTTGCGGTGTGTTTCTTATTTACAAAGTTTAAGCGCATGTTCGGTGATATGTCTGTTGGCGTTTTCACTGTCGGTGCTTGTACTTTGTTGAACAATGTTTCTTACATTGTAACACAGAACACACTTGGCATGTTGGGCTATGCAACTTTGTATTTCTTGTGCACTAAGGGTGTTAGATATATGTGGATTTGGCACTTGGGATTTTTGATCTCATATATTCTTATTGCACCATGGTGGGTTTTGATGGTTTATGCCTTTTCAGCCATTTTTGAGTTTATGCCTAACCTTTTTAAGCTTAAGGTTTCAACACAACTTTTTGAGGGCGACAAGTTCGTAGGCTCTTTTGAAAATGCTGCAGCAGGTACATTTGTGCTTGATATGCATGCCTATGAGAGACTTGCCAACTCTATCTCAACTGAAAAACTGCGTCAGTATGCTAGTACTTACAATAAGTACAAGTATTATTCAGGCAGTGCTTCAGAGGCTGATTACAGGCTTGCTTGTTTTGCCCATTTGGCCAAGGCTATGATGGATTATGCTTCTAATCATAACGATACGTTATACACACCACCCACTGTGAGTTACAATTCAACTCTACAGGCTGGCTTGCGTAAGATGGCACAACCTTCTGGTGTTGTTGAGAAGTGCATAGTCCGTGTTTGCTATGGTAATATGGCTCTTAATGGCCTATGGCTTGGCGATATTGTTATGTGCCCACGCCATGTTATAGCGTCTAGTACTACTAGCACTATAGATTATGATTATGCCCTTTCTGTTTTACGCCTCCACAACTTCTCCATTTCATCTGGGAATGTTTTCCTAGGTGTTGTGAGTGCAACTATGCGAGGTGCTTTGTTGCAGATAAAGGTTAATCAAAACAATGTCCACACGCCTAAGTACACCTATCGCACAGTTAGACCGGGTGAATCTTTTAATATTTTGGCGTGCTATGATGGTGCTGCAGCTGGTGTTTACGGCGTTAACATGCGCTCTAATTACACTATTAGAGGCTCGTTCATTAATGGCGCTTGTGGTTCACCTGGTTATAATATTAACAATGGTACCGTTGAGTTTTGCTATTTACACCAGCTTGAACTTGGTTCAGGCTGTCATGTTGGTAGCGACTTAGATGGTGTTATGTATGGTGGTTATGAGGACCAACCTACTTTGCAAGTTGAAGGCGCTAGTAGTCTGTTTACAGAGAATGTGTTGGCATTTCTTTATGCAGCACTCATTAATGGTTCTACCTGGTGGCTTAGTTCTTCTAGGATCGCTGTAGACAGGTTTAATGAGTGGGCTGTTCATAATGGTATGACAACAGTGGGCAATACTGATTGCTTTTCTATTCTTGCTGCTAAGACTGGAGTTGATGTACAACGTTTGTTGGCCTCAATCCAGTCTCTGCATAAGAATTTTGGTGGAAAGCAAATTCTTGGCCATACCTCGTTGACAGATGAGTTTACTACAGGTGAAGTTGTACGTCAAATGTATGGCGTTAATCTTCAGGGTGGCTATGTGTCACGCGCCTGCAGAAATGTCTTGCTGGTTGGTTCTTTTCTGACTTTCTTTTGGTCAGAATTAGTTTCCTACACTAAGTTCTTCTGGGTAAATCCTGGTTATGTTACACCTATGTTTGCGTGCTTGTCATTGCTATCCTCACTTTTGATGTTCACACTCAAGCATAAGACATTGTTTTTCCAGGTCTTCTTAATACCTGCTCTGATTGTTACATCTTGCATTAATTTGGCATTTGATGTTGAAGTCTACAACTATTTGGCAGAGCATTTTGATTACCATGTTTCTCTTATGGGTTTTAATGCACAAGGTCTTGTTAACATCTTTGTCTGCTTTGTTGTTACCATTTTACACGGCACATACACATGGCGCTTCTTTAATACACCTGCGAGTTCTGTCACTTATGTGGTAGCTTTGCTGACTGCGGCCTATAACTATTTTTACGCTAGTGACATTCTTAGTTGTGCTATGACACTATTTGCTAGTGTGACTGGCAACTGGTTCGTTGGTGCTGTTTGTTATAAAGTTGCTGTGTATATGGCCTTGAGATTTCCTACGTTCGTGGCTATTTTTGGTGATATTAAGAGTGTTATGTTCTGTTACCTTGTGTTGGGTTATTTTACCTGTTGCTTCTACGGTATTCTCTACTGGTTCAACAGGTTCTTTAAGGTTAGTGTAGGTGTCTATGACTATACTGTTAGTGCTGCTGAGTTTAAGTATATGGTTGCTAATGGCCTACGTGCACCAACTGGAACACTTGATTCACTACTGTTGTCCGCCAAATTGATTGGTATTGGTGGTGAGCGGAATATTAAGATTTCTTCCGTTCAGTCTAAACTGACTGATATTAAGTGTAGTAACGTTGTGCTTTTAGGCTGTCTTTCTAGCATGAATGTTTCAGCAAATTCAACAGAATGGGCTTATTGTGTTGACTTGCATAACAAGATCAACTTGTGTAATGACCCAGAAAAAGCGCAGGAAATGCTACTTGCTTTGTTGGCATTTTTCCTTAGTAAGAATAGTGCCTTTGGTTTAGACGACTTATTGGAATCTTATTTTAATGACAATAGTATGTTGCAGAGTGTTGCATCTACTTATGTAGGTTTGCCTTCCTATGTCATTTATGAAAATGCACGCCAACAGTATGAAGATGCTGTTAATAATGGTTCTCCACCTCAGTTGGTTAAGCAATTGCGCCATGCTATGAATGTAGCAAAGAGCGAATTTGACCGTGAGGCTTCTACTCAGCGTAAGCTTGATAGAATGGCGGAACAGGCTGCAGCACAGATGTATAAAGAGGCAAGGGCAGTTAATAGGAAGTCCAAAGTTGTAAGTGCTATGCATTCACTGCTTTTTGGTATGTTGAGACGTTTGGATATGTCTTCTGTAGACACCATTCTCAACTTGGCAAAGGATGGGGTGGTACCTCTGTCTGTCATACCGGCAGTCAGTGCTACTAAGCTTAACATTGTTACCTCTGATATCGATTCTTATAATCGTATCCAGCGTGAGGGATGTGTCCACTACGCTGGTACCATTTGGAATATAATTGATATCAAGGACAATGATGGCAAGGTGGTACACGTTAAGGAGGTAACCGCACAGAATGCTGAGTCCCTGTCATGGCCCCTGGTCCTTGGGTGTGAGCGTATTGTTAAGCTCCAGAATAATGAGATTATTCCTGGTAAGCTGAAGCAGCGCTCCATTAAGGCAGAAGGAGATGGCATAGTTGGAGAAGGTAAGGCACTTTACAATAATGAGGGTGGACGTACTTTTATGTATGCTTTCATTTCAGATAAACCGGACCTGCGTGTAGTTAAGTGGGAGTTCGATGGTGGTTGTAACACTATTGAGCTAGAACCACCACGTAAGTTCTTGGTGGATTCTCCTAATGGTGCACAGATCAAGTATCTCTACTTTGTTCGTAACCTTAACACGTTGCGTAGGGGTGCTGTTCTTGGCTACATAGGTGCCACTGTACGCTTGCAGGCTGGTAAACAAACAGAACAGGCTATTAACTCTTCATTGTTGACACTTTGCGCTTTCGCTGTGGATCCTGCTAAGACCTACATCGATGCTGTTAAAAGTGGTCACAAACCAGTAGGTAACTGTGTTAAGATGTTGGCCAATGGTTCTGGTAATGGACAAGCTGTTACTAATGGTGTGGAGGCTAGTACTAACCAGGATTCATATGGTGGTGCTTCCGTGTGTCTATATTGTAGAGCACATGTTGAGCATCCATCTATGGATGGTTTTTGCAGACTGAAAGGCAAGTATGTACAGGTGCCACTAGGTACAGTGGATCCTATACGTTTTGTACTTGAGAATGACGTTTGCAAGGTTTGTGGTTGTTGGCTGTCTAATGGCTGCACTTGTGACAGATCCATTATGCAAAGCACTGATATGGCTTATTTAAACGAGTACGGGGCTCTAGTGCAGCTCGACTAGAGCCCTGTAATGGTACTGATACACAACATGTGTATCGTGCTTTTGACATCTACAACAAGGATGTTGCTTGTCTAGGTAAATTCCTCAAGGTGAACTGTGTTCGCCTGAAGAATTTGGATAAGCATGATGCATTCTATGTTGTCAAAAGATGTACCAAGTCTGCGATGGAACACGAGCAATCCATCTATAGCAGACTTGAAAAGTGTGGGGCCATAGCCGAACACGATTTCTTCACTTGGAAGGATGGTCGTGCAATCTATGGTAACGTTTGTAGAAAGGATCTTACCGAGTATACTATGATGGATCTGTGTTACGCTTTACGTAACTTTGATGAAAACAATTGCGATGTTCTTAAGAGCATTTTGATTAAGGTAGGTGCTTGTGAGGAGTCCTACTTTAATAATAAAGTCTGGTTTGACCCTGTTGAAAATGAAGACATTCATCGTGTCTATGCATTGTTAGGTACCATTGTTGCACGTGCTATGCTTAAATGCGTTAAGTTCTGTGATGCAATGGTTGAACAAGGTATAGTTGGTGTTGTCACATTAGATAATCAGGATCTTAATGGTGATTTTTATGATTTTGGTGATTTTACTTGTAGTATCAAGGGAATGGGTGTACCCATTTGCACATCATATTACTCTTATATGATGCCTGTTATGGGTATGACTAATTGCCTTGCTAGTGAGTGTTTTGTTAAGAGTGATATATTTGGTGAGGATTTTAAGTCATATGACCTGCTGGAATATGATTTCACGGAGCATAAGACAGCACTCTTCAACAAGTATTTCAAGTATTGGGGACTGCAATACCACCCTAACTGTGTGGACTGCAGTGATGAGCAGTGCATAGTTCACTGTGCCAACTTCAATACGTTGTTTTCCACTACCATACCTATTACGGCATTTGGACCTTTGTGTCGCAAGTGCTGGATTGATGGTGTTCCACTGGTAACTACAGCTGGTTATCATTTTAAACAGTTAGGTATAGTTTGGAATAATGACCTCAACTTACACTCTAGCAGGCTCTCTATTAATGAATTACTCCAGTTTTGTAGTGATCCTGCACTGCTTATAGCATCATCACCAGCCCTTGTTGACCAGCGTACTGTTTGCTTTTCAGTTGCAGCGCTAGGTACAGGTATGACTAACCAGACTGTGAAACCTGGCCATTTCAATAAGGAGTTTTATGACTTCTTACTTGAGCAAGGTTTCTTCTCTGAGGGCTCTGAGCTTACTTTAAAGCACTTCTTCTTTGCACAGAAGGTTGATGCAGCTGTTAAGGATTTTGACTACTATAGGTATAATAGACCCACTGTTCTGGACATCTGCCAAGCTCGCGTCGTGTATCAAATAGTGCAACGCTATTTTGATATTTACGAGGGTGGTTGTATCACTGCTAAAGAAGTGGTTGTTACAAACCTTAACAAGAGCGCAGGCTATCCTTTGAACAAGTTTGGTAAAGCTGGTCTTTACTATGAGTCTTTATCCTATGAGGAACAGGATGAACTTTATGCTTATACTAAGCGTAACATCCTGCCCACTATGACACAGCTCAACCTTAAATACGCTATTAGTGGCAAAGAACGTGCACGCACAGTGGGTGGTGTCTCGCTTTTGTCAACCATGACCACTCGGCAGTATCATCAGAAACACCTTAAATCCATAGTTAATACTAGGGGCGCTTCGGTTGTTATTGGTACTACTAAGTTTTATGGTGGTTGGGACAATATGCTTAAGAACCTTATTGATGGTGTTGAAAATCCGTGTCTTATGGGTTGGGATTACCCAAAGTGCGATAGAGCACTGCCCAATATGATACGCATGATTTCAGCCATGATCTTAGGCTCTAAGCACACCACATGCTGCAGTTCTACTGACCGCTTTTTCAGGTTGTGCAATGAATTGGCTCAAGTCCTTACTGAGGTTGTTTATTCTAATGGAGGGTTTTATTTGAAGCCAGGTGGTACTACCTCTGGTGATGCAACCACCGCATATGCAAACTCAGTTTTCAATATCTTCCAAGCAGTAAGTGCCAATGTTAACAAACTTCTTAGTGTTGACAGCAATGTCTGTCATAACTTAGAAGTTAAGCAATTACAACGTAAGCTCTATGAGTGCTGTTATAGATCGACTATCGTCGATGACCAGTTCGTCGTTGAGTATTATGGTTACTTGCGTAAACATTTTTCAATGATGATTCTTTCTGATGATGGCGTTGTTTGTTATAACAATGACTATGCATCACTTGGTTATGTCGCGGATCTTAACGCATTCAAGGCTGTTTTGTATTACCAGAACAACGTCTTCATGAGCGCCTCTAAATGTTGGATCGAGCCTGACATTAATAAAGGTCCTCATGAATTTTGTTCGCAGCATACTATGCAGATTGTCGATAAGGAGGGTACTTATTACCTTCCTTACCCTGATCCTTCAAGAATCCTCTCTGCAGGTGTGTTTGTTGATGACGTTGTTAAAACTGATGCAGTTGTATTGCTTGAACGTTATGTGTCATTGGCTATAGATGCCTACCCGTTATCTAAGCATGAAAACCCTGAATATAAGAAGGTGTTTTATGTGCTTTTGGATTGGGTTAAGCATCTGTATAAAACTTTGAATGCTGGTGTGTTAGAGTCTTTTTCTGTCACACTTTTGGAAGATTCTACTGCTAAATTCTGGGATGAGAGCTTTTATGCCAACATGTATGAGAAATCTGCAGTTTTACAATCTGCAGGGCTTTGTGTTGTTTGTGGCTCTCAAACTGTTTTACGTTGTGGTGATTGTCTACGGCGTCCTATGCTTTGTACTAAGTGTGCTTATGATCATGTCATTGGAACAACTCACAAGTTCATTTTGGCCATCACTCCATATGTGTGTTGTGCTTCAGATTGTGGTGTCAATGATGTAACCAAGCTCTACTTAGGTGGTCTTAGTTACTGGTGTCATGAACACAAGCCACGTCTTGCATTCCCGTTGTGCTCTGCTGGTAATGTTTTTGGCTTATACAAAAATTCTGCTACCGGTTCACCCGATGTTGAAGACTTTAATCGCATTGCTACATCCGATTGGACTGATGTTTCTGACTACAGGTTGGCAAATGATGTCAAGGACTCATTGCGTCTATTTGCAGCGGAAACTATCAAGGCCAAGGAGGAGAGCGTTAAGTCATCCTATGCTTGTGCAACACTACATGAGGTTGTAGGACCTAAAGAGTTGTTGCTCAAATGGGAAGTCGGCAGACCCAAACCACCTCTTAATAGAAATTCGGTTTTCACTTGTTATCATATAACGAAGAACACCAAATTTCAAATCGGTGAGTTTGTGTTTGAGAAGGCAGAATATGATAATGACGCTGTAACATATAAAACTACCGCCACAACAAAACTTGTTCCTGGCATGGTTTTTGTGCTTACCTCACATAATGTTCAGCCATTGCGTGCACCAACCATTGCTAATCAAGAACGTTATTCCACTATACATAAGTTGCATCCTGCTTTTAACATACCTGAAGCTTATTCTAGCTTAGTGCCCTATTACCAATTGATTGGTAAGCAGAAGATTACAACTATCCAGGGACCCCCCGGTAGTGGTAAATCTCACTGTGTTATAGGGCTAGGTTTGTACTATCCAGGTGCACGTATTGTGTTTACAGCTTGTTCTCATGCAGCGGTCGATTCACTCTGTGTGAAGGCCTCCACTGCTTATAGCAATGACAAATGTTCACGCATCATACCACAGCGTGCTCGTGTTGAGTGTTATGATGGTTTCAAGTCTAATAATACTAGTGCTCAGTACCTTTTCTCCACTGTCAATGCTTTGCCAGAGTGTAATGCGGACATTGTTGTGGTGGATGAAGTTTCTATGTGCACTAATTATGACTTGTCTGTCATAAATCAGCGCATCAGCTATAGGCATGTAGTCTATGTTGGTGACCCTCAACAGCTGCCTGCACCACGTGTTATGATTTCACGTGGTACTTTGGAACCAAAGGACTACAATGTTGTCACTCAACGTATGTGTGCCCTTAAGCCTGATGTCTTCTTGCACAAGTGTTATCGCTGTCCTGCAGAGATAGTGCGCACTGTGTCTGAGATGGTCTATGAAAACCAATTCATTCCTGTGCACCCTGACAGCAAGCAGTGTTTTAAGATCTTTTGCAAGGGTAATGTGCAGGTTGATAACGGTTCAAGTATTAACCGCAGGCAATTGGATGTTGTGCGTATGTTTTTGGCTAAAAATCCTAGGTGGTCAAAGGCTGTTTTCATTTCCCCTTATAACAGCCAGAATTATGTTGCCAGCCGTTTGCTAGGTTTACAAATTCAGACAGTTGATTCATCCCAGGGTAGTGAGTATGACTATGTCATTTACGCACAAACCTCAGATACTGCCCATGCCAGTAATGTTAACAGGTTTAATGTTGCCATCACAAGGGCTAAGAAAGGCATATTATGTATAATGTGCGATAGGTCCCTTTTTGATCTGCTTAAATTTTTTGAGCTTAAATTGTCTGATTTGCAGGCTAATGAGGGTTGTGGTCTTTTTAAAGACTGTAGCAGAGGTGATGATTTGTTGCCACCGTCTCATGCTAATACCTTCATGTCTTTAGCGGACAATTTTAAGACTGATCAATATCTTGCTGTTCAAATAGGTGTTAATGGACCCATTAAATATGAGCATGTTATCTCGTTTATGGGTTTCCGTTTTGATATTAACATACCCAACCACCACACTCTCTTTTGCACACGCGACTTTGCCATGCGCAATGTTAGAGGTTGGTTGGGTTTTGACGTTGAAGGAGCACATGTTGTTGGCTCTAACGTCGGTACAAATGTCCCATTGCAATTAGGGTTTTCTAACGGTGTTGATTTTGTTGTCAGACCTGAAGGTTGCGTTGTAACTGAGTCTGGTGACTACATTAAACCCGTCAGAGCTCGTGCTCCACCAGGGGAACAATTCGCACACCTTTTGCCTTTACTTAAACGCGGCCAACCATGGGATGTGGTTCGCAAGCGTATAGTTCAAATGTGTAGTGACTACCTGGCTAACCTATCAGACATACTAATTTTTGTGTTGTGGGCTGGTGGTTTGGAGTTGACAACTATGCGTTACTTTGTCAAGATTGGACCAAGCAAGAGTTGTGATTGTGGTAAGGTTGCTACTTGTTACAATAGTGCGCTGCATACGTACTGTTGTTTCAAACATGCCCTTGGTTGTGATTATCTGTATAACCCATACTGTATTGATATACAGCAGTGGGGATACAAGGGATCACTTAGCCTTAACCACCATGAGCATTGTAATGTACATAGAAACGAGCATGTGGCTTCTGGTGATGCCATAATGACTCGCTGTCTGGCCATACATGATTGCTTTGTCAAGAACGTTGACTGGTCCATCACATACCCATTTATTGGTAATGAGGCTGTTATTAATAAGAGCGGCCGCATTGTGCAATCACACACTATGCGTTCAGTTCTTAAGTTATACAATCCAAAAGCCATATATGATATTGGCAATCCTAAGGGCATTAGATGCGCCGTAACGGATGCTAAGTGGTTCTGCTTTGACAAGAATCCTACTAATTCTAATGTCAAGACATTGGAGTATGACTATATAACACACGGCCAATTTGATGGGTTGTGCTTGTTTTGGAATTGCAATGTGGACATGTATCCAGAATTTTCTGTGGTCTGTCGTTTTGATACTCGCTGTAGGTCACCACTCAACTTGGAGGGTTGTAATGGTGGTTCACTGTATGTTAACAATCATGCATTCCACACACCGGCTTTTGACAAGCGTGCTTTTGCTAAGTTGAAGCCAATGCCATTTTTCTTCTATGATGATACTGAGTGTGACAAGTTACAGGACTCCATAAACTATGTTCCTCTTAGGGCTAGTAACTGCATTACTAAATGTAATGTTGGTGGAGCTGTTTGTAGTAAGCATTGTGCTATGTATCATAGCTATGTCAATGCTTACAACACCTTTACGTCAGCGGGCTTTACGATTTGGGTGCCCACTTCGTTTGACACCTATAATCTGTGGCAGACATTTAGTAACAATTTGCAAGGTCTTGAGAACATTGCTTTCAATGTTTTAAAGAAAGGATCTTTTGTTGGTGATGAAGGTGAGCTTCCTGTAGCTGTGGTCAATGACAAAGTGCTCGTTAGAGATGGTACTGTTGATACTCTTGTTTTTACAAACAAGACATCACTACCCACTAACGTAGCTTTTGAGTTGTATGCCAAGCGTAAGGTAGGACTCACCCCTCCCATTACGATCCTACGTAACTTGGGTGTTGTTTGTACATCTAAGTGTGTCATTTGGGACTATGAAGCCGAACGTCCACTTACTACTTTTACAAAGGATGTCTGTAAATATACCGACTTTGAGGGTGACGTTTGTACACTCTTTGATAACAGCATTGTTGGTTCATTAGAGCGATTCTCTATGACCCAGAATGCTGTGCTTATGTCACTTACAGCTGTTAAAAAGCTTACTGGCATAAAGTTAACTTATGGCTATCTTAATGGTGTCCCAGTTAACACACATGAAGATAAACCTTTTACTTGGTATATTTACACTAGGAAGAACGGCAAGTTCGAGGACTATCCTGATGGCTATTTTACCCAAGGTAGAACAACCGCTGATTTTAGCCCTCGTAGCGACATGGAAAAGGACTTCCTAAGTATGGATATGGGTTTGTTTATTAACAAGTACGGACTTGAAGATTACGGCTTTGAGCACGTTGTGTATGGTGATGTTTCAAAAACCACCCTTGGTGGTTTACATCTACTAATTTCGCAGGTGCGTCTGGCCTGTATGGGTGTGCTTAAAATAGACGAGTTTGTGTCTAGTAATGATAGCACGTTAAAGTCATGTACTGTTACATATGCTGACAACCCTAGTAGTAAGATGGTTTGCACGTATATGGATCTCCTGCTTGACGATTTTGTTAGCATTCTTAAATCTTTGGATTTGAGCGTTGTATCTAAGGTTCATGAAGTTATGGTTGATTGTAAAATGTGGAGGTGGATGTTGTGGTGTAAGGATCATAAACTCCAGACATTTTATCCACAACTTCAAGCCAGTGAATGGAAGTGTGGTTATTCCATGCCTTCTATTTACAAGATACAACGTATGTGCTTAGAACCTTGCAATCTCTATAACTATGGTGCTGGTGTTAAGTTACCTGATGGCATTATGTTTAACGTAGTTAAATACACACAGCTTTGTCAATATCTCAATAGCACCACAATGTGTGTACCCCATCACATGCGTGTGCTACATCTTGGTGCTGGCTCCGATAAGGGTGTTGCACCTGGCACGGCTGTCTTACGACGTTGGTTGCCACTGGATGCCATTATAGTTGACAATGATAGTGTGGATTACGTTAGCGATGCTGATTATAGTGTTACAGGAGATTGCTCTACCTTATACCTGTCAGATAAGTTTGACTTAGTTATATCTGATATGTATGATGGTAAGATTAAAAGTTGTGATGGGGAGAACGTGTCTAAAGAAGGCTTCTTTCCCTATATTAATGGTGTTATCACTGAAAAGTTGGCACTTGGTGGTACTGTAGCTATTAAGGTGACGGAGTTTAGTTGGAATAAGAAGTTGTATGAACTCATTCAGAAGTTTGAGTATTGGACAATGTTCTGTACCAGTGTTAACACGTCATCGTCAGAGGCATTTTTAATTGGTGTTCACTATTTAGGTGATTTTGCAAGTGGCGCTGTGATTGACGGCAACACCATGCATGCCAATTATATCTTCTGGCGTAATTCCACAATTATGACTATGTCTTACAACAGTGTGCTTGATTTAAGCAAGTTCAATTGTAAGCATAAGGCTACAGTTGTTGTTAATTTAAAAGACTCATCCATTAGTGATGTTGTGTTAGGCTTGTTGAAGAATGGTAAGTTGCTAGTGCGTAATAATGACGCCATTTGTGGCTTTTCTAATCATTTGGTCAACGTAAACAAATGA
Protein
MASNHVTLAFANDAEISAFGFCTASEAVSYYSEAAASGFMQCRFVSLDLADTVEGLLPEDYVMVVIGTTKLSAYVDTFGSRPRNICGWLLFSNCNYFLEELELTFGRRGGNIVPVDQYMCGADGKPVLQESEWEYTDFFADSEDGQLNIAGITYVKAWIVERSDVSYASQNLTSIKSITYCSTYEHTFLDGTAMKVARTPKIKKNVVLSEPLATIYREIGSPFVDNGSDARSIIRRPVFLHAFVKCKCGSYHWTVGDWTSYVSTCCGFKCKPVLVASCSAMPGSVVVTRAGAGTGVKYYNNMFLRHVADIDGLAFWRILKVQSKDDLACSGKFLEHHEEGFTDPCYFLNDSSLATKLKFDILSGKFSDEVKQAIIAGHVVVGSALVDIVDDALGQPWFIRKLGDLASAPWEQLKAVVRGLGLLSDEVVLFGKRLSCATLSIVNGVFEFLADVPEKLAAAVTVFVNFLNEFFESACDCLKVGGKTFNKVGSYVLFDNALVKLVKAKARGPRQAGICEVRYTSLVVGSTTKVVSKRVENANVNLVVVDEDVTLNTTGRTVVVDGLAFFESDGFYRHLADADVVIEHPVYKSACELKPVFECDPIPDFPLPVAASVAELCVQTDLLLKNYNTPYKTYSCVVRGDKCCITCTLQFKAPSYVEDAVNFVDLCTKNIGTAGFHEFYITAHEQQDLQGFLTTCCTMSGFECFMPTIPQCPAVLEEIDGGSIWRSFITGLNTMWDFCKRLKVSFGLDGIVVTVARKFKRLGALLAEMYNTYLSTVVENLVLAGVSFKYYATSVPKIVLGGCFHSVKSVFASVFQIPVQAGIEKFKVFLNCVHPVVPRVIETSFVELEETTFKPPALNGGIAIVDGFAFYYDGTLYYPTDGNSVVPICFKKKGGGDVKFSDEVSVKTIDPVYKVSLEFEFESETIMAVLNKAVGNRIKVTGGWDDVVEYINVAIEVLKDHVEVPKYYIYDEEGGTDPNLPVMVSQWPLNDDTISQDLLDVEVVTDAPIDSEGDEVDSSAPEKVADVANSEPGDDGLPVAPETNVESEVEEVAATLSFIKDTPSTVTKDPFAFDFVSYGGLKVLRQSHNNCWVTSTLVQLQLLGIVDDPAMELFSAGRVGPMVRKCYESQKAILGSLGDVSACLESLTKDLHTLKITCSVVCGCGTGERIYEGCAFRMTPTLEPFPYGACAQCAQVLMHTFKSIVGTGIFCRDTTALSLDSLVVKPLCAAAFIGKDSGHYVTNFYDAAMAIDGYGRHQIKYDTLNTICVKDVNWTAPLVPAVDSVVEPVVKPFYSYKNVDFYQGDFSDLVKLPCDFVVNAANEKLSHGGGIAKAIDVYTKGMLQKCSNDYIKAHGPIKVGRGVMLEALGLKVFNVVGPRKGKHAPELLVKAYKSVFANSGVALTPLISVGIFSVPLEESLSAFLACVGDRHCKCFCYGDKEREAIIKYMDGLVDAIFKEALVDTTPVQEDVQQVSQKPVLPNFEPFRIEGAHAFYECNPEGLMSLGADKLVLFTNSNLDFCSVGKCLNDVTSGALLEAINVFKKSNKTVPAGNCVTLDCANMISITMVVLPFDGDANYDKNYARAVVKVSKLKGKLVLAVDDATLYSKLSHLSVLGFVSTPDDVERFYANKSVVIKVTEDTRSVKAVKVESTATYGQQIGPCLVNDTVVTDNKPVVADVVAKVVPNANWDSHYGFDKAGEFHMLDHTGFTFPSEVVNGRRVIKTTDNNCWVNVTCLQLQFARFRFKSAGLQAMWESYCTGDVAMFVHWLYWLTGVDKGQPSDSENALNMLSKYIVPAGSVTIERVTHDGCCCSKRVVTAPVVNASVLKLGVEDGLCPHGLNYIGKVVVVKGTTIVVNVGKPVVAPSHLFLKGVSYTTFLDNGNGVVGHYTVFDHGTGMVHDGDAFVPGDLNVSPVTNVVVSEQTAVVIKDPVKKAELDATKLLDTMNYASERFFSFGDFMSRNLITVFLYILSILGLCFRAFRKRDVKVLAGVPQRTGIILRKSMRYNAKALGVFFKLKLYWFKVLGKFSLGIYALYALLFMTIRFTPIGSPVCDDVVAGYANSSFDKNEYCNSVICKVCLYGYQELSDFSHTQVVWQHLRDPLIGNVMPFFYLAFLAIFGGVYVKAITLYFIFQYLNSLGVFLGLQQSIWFLQLVPFDVFGDEIVVFFIVTRVLMFIKHVCLGCDKASCVACSKSARLKRVPVQTIFQGTSKSFYVHANGGSKFCKKHNFFCLNCDSYGPGCTFINDVIATEVGNVVKLNVQPTGPATILIDKVEFSNGFYYLYSGDTFWKYNFDITDSKYTCKEALKNCSIITDFIVFNNNGSNVNQVKNACVYFSQMLCKPVKLVDSALLASLSVDFGASLHSAFVSVLSNSFGKDLSSCNDMQDCKSTLGFDDVPLDTFNAAVAEAHRYDVLLTDMSFNNFTTSYAKPEEKFPVHDIATCMRVGAKIVNHNVLVKDSIPVVWLVRDFIALSEETRKYIIRTTKVKGITFMLTFNDCRMHTTIPTVCIANKKGAGLPSFSKVKKFFWFLCLFIVAAFFALSFLDFSTQVSSDSDYDFKYIESGQLKTFDNPLSCVHNVFINFDQWHDAKFGFTPVNNPSCPIVVGVSDEARTVPGIPAGVYLAGKTLVFAINTIFGTSGLCFDASGVADKGACIFNSACTTLSGLGGTAVYCYKNGLVEGAKLYSELAPHSYYKMVDGNAVSLPEIISRGFGIRTIRTKAMTYCRVGQCVQSAEGVCFGADRFFVYNAESGSDFVCGTGLFTLLMNVISVFSKTVPVTVLSGQILFNCIIAFVAVAVCFLFTKFKRMFGDMSVGVFTVGACTLLNNVSYIVTQNTLGMLGYATLYFLCTKGVRYMWIWHLGFLISYILIAPWWVLMVYAFSAIFEFMPNLFKLKVSTQLFEGDKFVGSFENAAAGTFVLDMHAYERLANSISTEKLRQYASTYNKYKYYSGSASEADYRLACFAHLAKAMMDYASNHNDTLYTPPTVSYNSTLQAGLRKMAQPSGVVEKCIVRVCYGNMALNGLWLGDIVMCPRHVIASSTTSTIDYDYALSVLRLHNFSISSGNVFLGVVSATMRGALLQIKVNQNNVHTPKYTYRTVRPGESFNILACYDGAAAGVYGVNMRSNYTIRGSFINGACGSPGYNINNGTVEFCYLHQLELGSGCHVGSDLDGVMYGGYEDQPTLQVEGASSLFTENVLAFLYAALINGSTWWLSSSRIAVDRFNEWAVHNGMTTVGNTDCFSILAAKTGVDVQRLLASIQSLHKNFGGKQILGHTSLTDEFTTGEVVRQMYGVNLQGGYVSRACRNVLLVGSFLTFFWSELVSYTKFFWVNPGYVTPMFACLSLLSSLLMFTLKHKTLFFQVFLIPALIVTSCINLAFDVEVYNYLAEHFDYHVSLMGFNAQGLVNIFVCFVVTILHGTYTWRFFNTPASSVTYVVALLTAAYNYFYASDILSCAMTLFASVTGNWFVGAVCYKVAVYMALRFPTFVAIFGDIKSVMFCYLVLGYFTCCFYGILYWFNRFFKVSVGVYDYTVSAAEFKYMVANGLRAPTGTLDSLLLSAKLIGIGGERNIKISSVQSKLTDIKCSNVVLLGCLSSMNVSANSTEWAYCVDLHNKINLCNDPEKAQEMLLALLAFFLSKNSAFGLDDLLESYFNDNSMLQSVASTYVGLPSYVIYENARQQYEDAVNNGSPPQLVKQLRHAMNVAKSEFDREASTQRKLDRMAEQAAAQMYKEARAVNRKSKVVSAMHSLLFGMLRRLDMSSVDTILNLAKDGVVPLSVIPAVSATKLNIVTSDIDSYNRIQREGCVHYAGTIWNIIDIKDNDGKVVHVKEVTAQNAESLSWPLVLGCERIVKLQNNEIIPGKLKQRSIKAEGDGIVGEGKALYNNEGGRTFMYAFISDKPDLRVVKWEFDGGCNTIELEPPRKFLVDSPNGAQIKYLYFVRNLNTLRRGAVLGYIGATVRLQAGKQTEQAINSSLLTLCAFAVDPAKTYIDAVKSGHKPVGNCVKMLANGSGNGQAVTNGVEASTNQDSYGGASVCLYCRAHVEHPSMDGFCRLKGKYVQVPLGTVDPIRFVLENDVCKVCGCWLSNGCTCDRSIMQSTDMAYLNRVRGSSAARLEPCNGTDTQHVYRAFDIYNKDVACLGKFLKVNCVRLKNLDKHDAFYVVKRCTKSAMEHEQSIYSRLEKCGAIAEHDFFTWKDGRAIYGNVCRKDLTEYTMMDLCYALRNFDENNCDVLKSILIKVGACEESYFNNKVWFDPVENEDIHRVYALLGTIVARAMLKCVKFCDAMVEQGIVGVVTLDNQDLNGDFYDFGDFTCSIKGMGVPICTSYYSYMMPVMGMTNCLASECFVKSDIFGEDFKSYDLLEYDFTEHKTALFNKYFKYWGLQYHPNCVDCSDEQCIVHCANFNTLFSTTIPITAFGPLCRKCWIDGVPLVTTAGYHFKQLGIVWNNDLNLHSSRLSINELLQFCSDPALLIASSPALVDQRTVCFSVAALGTGMTNQTVKPGHFNKEFYDFLLEQGFFSEGSELTLKHFFFAQKVDAAVKDFDYYRYNRPTVLDICQARVVYQIVQRYFDIYEGGCITAKEVVVTNLNKSAGYPLNKFGKAGLYYESLSYEEQDELYAYTKRNILPTMTQLNLKYAISGKERARTVGGVSLLSTMTTRQYHQKHLKSIVNTRGASVVIGTTKFYGGWDNMLKNLIDGVENPCLMGWDYPKCDRALPNMIRMISAMILGSKHTTCCSSTDRFFRLCNELAQVLTEVVYSNGGFYLKPGGTTSGDATTAYANSVFNIFQAVSANVNKLLSVDSNVCHNLEVKQLQRKLYECCYRSTIVDDQFVVEYYGYLRKHFSMMILSDDGVVCYNNDYASLGYVADLNAFKAVLYYQNNVFMSASKCWIEPDINKGPHEFCSQHTMQIVDKEGTYYLPYPDPSRILSAGVFVDDVVKTDAVVLLERYVSLAIDAYPLSKHENPEYKKVFYVLLDWVKHLYKTLNAGVLESFSVTLLEDSTAKFWDESFYANMYEKSAVLQSAGLCVVCGSQTVLRCGDCLRRPMLCTKCAYDHVIGTTHKFILAITPYVCCASDCGVNDVTKLYLGGLSYWCHEHKPRLAFPLCSAGNVFGLYKNSATGSPDVEDFNRIATSDWTDVSDYRLANDVKDSLRLFAAETIKAKEESVKSSYACATLHEVVGPKELLLKWEVGRPKPPLNRNSVFTCYHITKNTKFQIGEFVFEKAEYDNDAVTYKTTATTKLVPGMVFVLTSHNVQPLRAPTIANQERYSTIHKLHPAFNIPEAYSSLVPYYQLIGKQKITTIQGPPGSGKSHCVIGLGLYYPGARIVFTACSHAAVDSLCVKASTAYSNDKCSRIIPQRARVECYDGFKSNNTSAQYLFSTVNALPECNADIVVVDEVSMCTNYDLSVINQRISYRHVVYVGDPQQLPAPRVMISRGTLEPKDYNVVTQRMCALKPDVFLHKCYRCPAEIVRTVSEMVYENQFIPVHPDSKQCFKIFCKGNVQVDNGSSINRRQLDVVRMFLAKNPRWSKAVFISPYNSQNYVASRLLGLQIQTVDSSQGSEYDYVIYAQTSDTAHASNVNRFNVAITRAKKGILCIMCDRSLFDLLKFFELKLSDLQANEGCGLFKDCSRGDDLLPPSHANTFMSLADNFKTDQYLAVQIGVNGPIKYEHVISFMGFRFDINIPNHHTLFCTRDFAMRNVRGWLGFDVEGAHVVGSNVGTNVPLQLGFSNGVDFVVRPEGCVVTESGDYIKPVRARAPPGEQFAHLLPLLKRGQPWDVVRKRIVQMCSDYLANLSDILIFVLWAGGLELTTMRYFVKIGPSKSCDCGKVATCYNSALHTYCCFKHALGCDYLYNPYCIDIQQWGYKGSLSLNHHEHCNVHRNEHVASGDAIMTRCLAIHDCFVKNVDWSITYPFIGNEAVINKSGRIVQSHTMRSVLKLYNPKAIYDIGNPKGIRCAVTDAKWFCFDKNPTNSNVKTLEYDYITHGQFDGLCLFWNCNVDMYPEFSVVCRFDTRCRSPLNLEGCNGGSLYVNNHAFHTPAFDKRAFAKLKPMPFFFYDDTECDKLQDSINYVPLRASNCITKCNVGGAVCSKHCAMYHSYVNAYNTFTSAGFTIWVPTSFDTYNLWQTFSNNLQGLENIAFNVLKKGSFVGDEGELPVAVVNDKVLVRDGTVDTLVFTNKTSLPTNVAFELYAKRKVGLTPPITILRNLGVVCTSKCVIWDYEAERPLTTFTKDVCKYTDFEGDVCTLFDNSIVGSLERFSMTQNAVLMSLTAVKKLTGIKLTYGYLNGVPVNTHEDKPFTWYIYTRKNGKFEDYPDGYFTQGRTTADFSPRSDMEKDFLSMDMGLFINKYGLEDYGFEHVVYGDVSKTTLGGLHLLISQVRLACMGVLKIDEFVSSNDSTLKSCTVTYADNPSSKMVCTYMDLLLDDFVSILKSLDLSVVSKVHEVMVDCKMWRWMLWCKDHKLQTFYPQLQASEWKCGYSMPSIYKIQRMCLEPCNLYNYGAGVKLPDGIMFNVVKYTQLCQYLNSTTMCVPHHMRVLHLGAGSDKGVAPGTAVLRRWLPLDAIIVDNDSVDYVSDADYSVTGDCSTLYLSDKFDLVISDMYDGKIKSCDGENVSKEGFFPYINGVITEKLALGGTVAIKVTEFSWNKKLYELIQKFEYWTMFCTSVNTSSSEAFLIGVHYLGDFASGAVIDGNTMHANYIFWRNSTIMTMSYNSVLDLSKFNCKHKATVVVNLKDSSISDVVLGLLKNGKLLVRNNDAICGFSNHLVNVNK
Summary
Function
The non-structural protein 1 (nsp1) protein plays a role in the inhibition of host interferon and pro-inflammatory cytokines production. Suppresses host RELA/p65 activation by blocking NFKBIA phosphorylation (PubMed:28715653). Targets also the RLR pathway downstream of the IRF3 activation by targeting host CREBBP to proteasomal degradation (PubMed:26773386).
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Catalytic Activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H2O = ADP + H(+) + phosphate
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H2O = ADP + H(+) + phosphate
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
ATP-binding
Endonuclease
Exonuclease
Helicase
Host cytoplasm
Host membrane
Host nucleus
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Methyltransferase
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Nuclease
Nucleotide-binding
Nucleotidyltransferase
Protease
Repeat
Ribosomal frameshifting
RNA-binding
RNA-directed RNA polymerase
Thiol protease
Transferase
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
Zinc
Zinc-finger
Feature
chain Non-structural protein 1
Uniprot
EMBL
Proteomes
PRIDE
Pfam
Interpro
IPR014828
NSP7
IPR027352 CV_ZBD
IPR036333 NSP10_sf
IPR009469 RNA_pol_N_coronovir
IPR032505 Corona_NSP4_C
IPR009466 NSP11
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR029063 SAM-dependent_MTases
IPR027351 (+)RNA_virus_helicase_core_dom
IPR018995 RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR041679 DNA2/NAM7-like_AAA
IPR011050 Pectin_lyase_fold/virulence
IPR037227 EndoU-like
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR013016 Peptidase_C30/C16
IPR014827 Viral_protease
IPR009461 Coronavirus_NSP16
IPR002589 Macro_dom
IPR027417 P-loop_NTPase
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR042515 Nsp15_N
IPR027352 CV_ZBD
IPR036333 NSP10_sf
IPR009469 RNA_pol_N_coronovir
IPR032505 Corona_NSP4_C
IPR009466 NSP11
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR029063 SAM-dependent_MTases
IPR027351 (+)RNA_virus_helicase_core_dom
IPR018995 RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR041679 DNA2/NAM7-like_AAA
IPR011050 Pectin_lyase_fold/virulence
IPR037227 EndoU-like
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR013016 Peptidase_C30/C16
IPR014827 Viral_protease
IPR009461 Coronavirus_NSP16
IPR002589 Macro_dom
IPR027417 P-loop_NTPase
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR042515 Nsp15_N
SUPFAM
ProteinModelPortal
PDB
6NUS
E-value=0.0 Score=1165 Identity=59.31%
Cov(Q)=13.77% Cov(P)=97.80%
3D Structure
Ontologies
KEGG
GO
GO:0033644 C:host cell membrane
GO:0004519 F:endonuclease activity
GO:0019079 P:viral genome replication
GO:0008168 F:methyltransferase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0003724 F:RNA helicase activity
GO:0039520 P:induction by virus of host autophagy
GO:0030430 C:host cell cytoplasm
GO:0039644 P:suppression by virus of host NF-kappaB transcription factor activity
GO:0005524 F:ATP binding
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0006351 P:transcription, DNA-templated
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0003723 F:RNA binding
GO:0016896 F:exoribonuclease activity, producing 5'-phosphomonoesters
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0042025 C:host cell nucleus
GO:0004519 F:endonuclease activity
GO:0019079 P:viral genome replication
GO:0008168 F:methyltransferase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0003724 F:RNA helicase activity
GO:0039520 P:induction by virus of host autophagy
GO:0030430 C:host cell cytoplasm
GO:0039644 P:suppression by virus of host NF-kappaB transcription factor activity
GO:0005524 F:ATP binding
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0006351 P:transcription, DNA-templated
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0003723 F:RNA binding
GO:0016896 F:exoribonuclease activity, producing 5'-phosphomonoesters
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0042025 C:host cell nucleus
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host cytoplasm
Host nucleus
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 2 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 2 publications.
Host nucleus
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 2 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 2 publications.
Topology
Length:
6781
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
413.998979999994
Exp number, first 60 AAs:
0.01219
Total prob of N-in:
0.00104
outside
1 - 1962
TMhelix
1963 - 1982
inside
1983 - 2019
TMhelix
2020 - 2042
outside
2043 - 2102
TMhelix
2103 - 2125
inside
2126 - 2131
TMhelix
2132 - 2151
outside
2152 - 2154
TMhelix
2155 - 2177
inside
2178 - 2527
TMhelix
2528 - 2550
outside
2551 - 2622
TMhelix
2623 - 2645
inside
2646 - 2656
TMhelix
2657 - 2676
outside
2677 - 2752
TMhelix
2753 - 2775
inside
2776 - 2781
TMhelix
2782 - 2804
outside
2805 - 2829
TMhelix
2830 - 2852
inside
2853 - 2864
TMhelix
2865 - 2887
outside
2888 - 3331
TMhelix
3332 - 3354
inside
3355 - 3360
TMhelix
3361 - 3383
outside
3384 - 3397
TMhelix
3398 - 3420
inside
3421 - 3426
TMhelix
3427 - 3449
outside
3450 - 3458
TMhelix
3459 - 3481
inside
3482 - 3500
TMhelix
3501 - 3520
outside
3521 - 6781
