CoVDB Coronavirus Database Go to the lastest version >>
Strain
EUROPE_MURINE_2004_AY700211 (Region: Europe;  Strain: Murine hepatitis virus strain A59, complete genome.;  Date: 2004)
Gene
hemagglutinin-esterase
Description
Annotated in NCBI,  hemagglutinin-esterase
GenBank Accession
Full name
Hemagglutinin-esterase      
Alternative Name
E3 glycoprotein
 

Sequence

CDS
ATGGGCAATAAAGCTCGATTTTATGCCCGAGTGTATGAGAAGATGGCCCAATATAGGAGCCTATCGTTTGTTAATGTGTCTTATGCCTATGGAGGTAATGCAAAGCCCGCCTCCATTTGCAAAGACAATACTTTAACACTCAATAACCCCACCTTCATATCGAAGGAGTCTAATTATGTTGATTATTACTATGAGAGTGAGGCTAATTTCACACTAGAAGGTTGTGATGAATTTATAGTACCGCTCTGTGGTTTTAATGGCCATTCCAAGGGCAGCTCTTCGGATGCTGCCAATAAATATTATACTGACTCTCAGAGTTACTATAATATGGATATTGGTGTCTTATATGGGTTCAATTCGACCTTGGATGTTGGCAACACTGCTAAGGATCCGGGTCTTGATCTCACTTGTAGGTATCTTGCATTGACTCCTGGTAATTATAAGGCTGTGTCCTTAGAATATTTGTTAAGCTTACCCTCAAAGGCTATTTGCCTCCATAAGACAAAGCGCTTTATGCCTGTGCAGGTAGTTGACTCAAGGTGGAGTAGCATCCGCCAGTCAGACAATATGACCGCTGCAGCCTGTCAGCTGCCATATTGTTTCTTTCGCAACACATCTGCGAATTATAGTGGTGGCACACATGATGCGCACCATGGTGATTTTCATTTCAGGCAGTTATTGTCTGGTTTGTTATATAATGTTTCCTGTATTGCCCAGCAGGGTGCATTTCTTTATAATAATGTTAGTTCCTCTTGGCCAGCCTATGGGTACGGTCATTGTCCAACGGCAGCTAACATTGGTTATATGGCACCTGTTTGTATCTATGACCCTCTCCCGGTCATACTGCTAGGTGTGTTATTGGGTATAGCTGTGTTGACTATTGTGTTTTTGATGTTTTATTTTATGACGGATAGCGGTGTTAGATTGCATGAGGCATAA
Protein
MGNKARFYARVYEKMAQYRSLSFVNVSYAYGGNAKPASICKDNTLTLNNPTFISKESNYVDYYYESEANFTLEGCDEFIVPLCGFNGHSKGSSSDAANKYYTDSQSYYNMDIGVLYGFNSTLDVGNTAKDPGLDLTCRYLALTPGNYKAVSLEYLLSLPSKAICLHKTKRFMPVQVVDSRWSSIRQSDNMTAAACQLPYCFFRNTSANYSGGTHDAHHGDFHFRQLLSGLLYNVSCIAQQGAFLYNNVSSSWPAYGYGHCPTAANIGYMAPVCIYDPLPVILLGVLLGIAVLTIVFLMFYFMTDSGVRLHEA

Summary

Function
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
Catalytic Activity
H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
Subunit
Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.
Miscellaneous
Readthrough of the terminator UGA may occur between the codons for Ile-14 and Cys-16.
The sequence shown is that of Quebec reference strain.
Similarity
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.
Keywords
Disulfide bond   Glycoprotein   Hemagglutinin   Host cell membrane   Host membrane   Hydrolase   Membrane   Reference proteome   Signal   Transmembrane   Transmembrane helix   Viral envelope protein   Virion   3D-structure   Direct protein sequencing  
Feature
chain  Hemagglutinin-esterase
EC Number
3.1.1.53
EMBL
AY700211    AAU06355.1    KF268336    AGT17715.1    KF268337    KF268338    + More
KF268339    AGT17726.1    AGT17737.1    AGT17748.1    M23256    AF029248    JQ173883    AFD97612.1    GU593319    ADI59789.1    AY771997    AB008939    M64316    MF416379    AWB14619.1    FJ647227    ACN89775.1    FJ647219    ACN89693.1    FJ647226    ACN89762.1    FJ647222    ACN89727.1    JX169866    JX169867    AFO11506.1    AFO11516.1    D00764    M28348    DQ198384    ABA55820.1    AF207551    FJ938068    ACT11041.1    DQ198387    JF792617    ABA55823.1    AFG25766.1    DQ198383    ABA55819.1    DQ198385    ABA55821.1    JF792616    AFG25756.1    DQ198386    ABA55822.1    AJ005960    KF294372    AID16660.1    AY771998    AAW47239.1    AF091734    AB551247    BAJ04695.1    KY370051    ATP66772.1    KF294370    AID16648.1    KY370043    ATP66726.1    KY370049    ATP66761.1    MH687970    AYR18615.1    MH687968    AYR18598.1    KY370048    ATP66755.1    KF294371    AID16654.1    DQ682406    ABG78747.1    MK045998    MK045999    QBG67067.1    QBG67068.1    EU019216    ABV74053.1    EF445634    ABP73655.1    KX432213    AQT26497.1    KX982264    ARD05998.1    EU999954    ACL12995.1    MH249786    QAY30019.1    GQ918141    ACX46839.1    MG757138    AVI14999.1    GQ918143    ACX46859.1    FJ938067    ACT11029.1    FJ415324    ACJ35485.1    M80842    U00735    S50936    FJ938064    ACT10994.1    FJ938065    ACT11006.1    EF424620    ABP38286.1    MN514963    QEY10632.1    L38962    L38963    U06093    AF239306    AF239307    AF230523    AF230524    AF230525    AF230526    AF230527    AF230528    AF339836    AF220295    S69264    M76372    DQ389646    ABD18922.1    MK046001    QBG67070.1    MK045994    QBG67063.1    GQ918142    JX860640    ACX46849.1    AFW97359.1    AH014866    AH014870    AAY33928.1    AAY33940.1    AH014871    AAY33943.1    MH203059    AXP11691.1    DQ994168    ABI98029.1    EU401979    ACB30199.1    DQ389643    DQ389644    DQ389645    ABD18919.1    ABD18920.1    ABD18921.1    MK095141    MK095143    MK095144    AZS64188.1    AZS64190.1    AZS64191.1    FJ425189    ACJ66999.1    EF424624    ABP38338.1    FJ425186    ACJ66968.1    EF424623    ABP38323.1    FJ425185    ACJ66956.1    FJ425187    ACJ66975.1    EF424621    ABP38304.1    FJ425184    ACJ66944.1    EF424622    ABP38312.1    FJ425190    ACJ67011.1    DQ389651    EU401975    EU401976    HM573321    HM573322    HM573323    HM573324    HM573325    MH043953    MH043954    MK045992    MK045993    MK046000    MK046002    ABD18927.1    ACB30195.1    ACB30196.1    ADP21330.1    ADP21331.1    ADP21332.1    ADP21333.1    ADP21334.1    AVZ61108.1    AVZ61118.1    QBG67061.1    QBG67062.1    QBG67069.1    QBG67071.1    AF391541    DQ389647    ABD18923.1    AF058944    DQ389648    ABD18924.1    EF424618    ABP38271.1    EF424617    ABP38257.1    EU401977    EU401978    AB354579    ACB30197.1    ACB30198.1    BAF75630.1    AF239308    AF239309    MK095147    AZS64194.1    EF424619    ABP38274.1    AF391542    FJ938063    ACT10982.1    EF424615    ABP38240.1    DQ811784    ABG89283.1    MH475914    QBH22432.1    MH043955    AVZ61128.1    MG757140    MG757142    AVI15021.1    AVI15043.1    MG757139    MG757141    AVI15010.1    AVI15032.1    M84486    AF058942    KU558922    ANJ04716.1    MF593476    ATI09448.1   
Pfam
PF03996   Hema_esterase
PF02710   Hema_HEFG
Interpro
IPR008980   Capsid_hemagglutn
IPR007142   Hemagglutn-estrase_core
IPR003860   Hemagglutn-estrase_hemagglutn
IPR042545   HEMA
SUPFAM
SSF49818   SSF49818
PDB
4C7W     E-value=0,     Score=1777

Ontologies

KEGG

Subcellular Location

From MSLVP
Single-Pass Membrane
From Uniprot
Virion membrane   In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.   With evidence from 1 publications.
Host cell membrane   In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.   With evidence from 1 publications.

Topology

Length:
312
Number of predicted TMHs:
1
Exp number of AAs in TMHs:
27.83494
Exp number, first 60 AAs:
0.11792
Total prob of N-in:
0.66479
inside
1  -  277
TMhelix
278  -  300
outside
301  -  312
 
 

Population Genetic Test Statistics

Genomic alignment in the CDS region

 
 

Multiple alignment of Orthologues

 
 

Gene Tree

 
 

Orthologous in Strains

Strain Availability Status Gene
CHINA_HS_2019_MN908947    
CHINA_AVIAN_2008_NC_016995    
CHINA_AVIAN_2007_NC_016991    
CHINA_MURINE_2015_NC_035191    
CANADA_AVIAN_2007_NC_010800    
USA_PIG_2000_NC_038861    
CHINA_AVIAN_2007_NC_011549    
ITALY_PIG_2009_NC_028806    
GERMANY_PIG_2012_LT545990    
CHINA_AVIAN_2007_NC_016992    
CHINA_BAT_2005_NC_009657    
CANADA_HS_2003_NC_004718    
CHINA_BAT_2014_NC_030886    
CHINA_BAT_2005_NC_018871    
USA_MURINE_2009_NC_012936 Protein
YP_003029847.1
CHINA_RABBIT_2006_NC_017083    
UK_PIG_2000_NC_003436    
ROMANIA_PIG_2015_LT898435    
ROMANIA_PIG_2015_LT898436    
GERMANY_PIG_2015_LT898444    
GERMANY_PIG_2015_LT898414    
GERMANY_PIG_2015_LT898439    
GERMANY_PIG_2015_LT898413    
GERMANY_PIG_2015_LT898412    
GERMANY_PIG_2015_LT898411    
GERMANY_PIG_2015_LT898416    
GERMANY_PIG_2015_LT898443    
GERMANY_PIG_2015_LT898420    
GERMANY_PIG_2015_LT898408    
GERMANY_PIG_2015_LT898423    
GERMANY_PIG_2015_LT898432    
GERMANY_PIG_2015_LT898409    
GERMANY_PIG_2015_LT898425    
GERMANY_PIG_2015_LT898446    
GERMANY_PIG_2014_LT898438    
GERMANY_PIG_2014_LT898440    
GERMANY_PIG_2014_LT900501    
GERMANY_PIG_2014_LT898427    
GERMANY_PIG_2014_LT898415    
GERMANY_PIG_2014_LT898421    
GERMANY_PIG_2014_LT898431    
GERMANY_PIG_2014_LT898410    
GERMANY_PIG_2014_LT900498    
GERMANY_PIG_2014_LT898430    
GERMANY_PIG_1978_LT897799    
GERMANY_PIG_2014_LT898447    
GERMANY_PIG_2014_LT898426    
GERMANY_PIG_2014_LT898417    
GERMANY_PIG_2014_LT900500    
GERMANY_PIG_2014_LT898445    
AUSTRIA_PIG_2015_LT898418    
AUSTRIA_PIG_2015_LT898441    
AUSTRIA_PIG_2015_LT898433    
AUSTRIA_PIG_2015_LT900502    
GERMANY_PIG_2015_LT900499    
BELGIUM_PIG_1980_LT906620    
BELGIUM_PIG_1977_LT905450    
SWITZERLAND_PIG_2003_LT905451    
BELGIUM_PIG_1978_LT906581    
UK_PIG_1987_LT906582    
CHINA_PIG_2009_NC_016990    
CHINA_PIG_2010_NC_039208    
KENYA_BAT_2010_KY073745    
KENYA_BAT_2010_NC_032107    
CHINA_AVIAN_2007_NC_016994    
EUROPE_MURINE_2004_AY700211 Protein
AAU06355.1
CHINA_AVIAN_2007_NC_011550    
USA_MURINE_1997_NC_001846    
USA_MURINE_1998_NC_023760    
MID_EAST_HS_2012_NC_019843    
CHINA_AVIAN_2007_NC_016993    
CHINA_MURINE_2013_NC_032730    
USA_HS_2004_AY585228    
NETHERLAND_HS_2004_NC_005831    
CHINA_HS_2004_NC_006577    
EUROPE_HS_2000_NC_002645    
NETHERLAND_FERRET_2010_NC_030292    
JAPAN_FERRET_2013_LC119077    
USA_FELINE_2005_NC_002306    
CHINA_MURINE_2011_NC_034972    
CHINA_AVIAN_2007_NC_016996    
ARABIA_CAMEL_2015_NC_028752    
CHINA_AVIAN_2007_NC_011547    
CHINA_BAT_2012_NC_028824    
CHINA_BAT_2013_NC_028814    
CHINA_BAT_2013_NC_028833    
CHINA_BAT_2011_NC_028811    
USA_BOVIN_2001_NC_003045    
CHINA_MURINE_2012_NC_026011    
GERMANY_ERINACEINAE_2012_NC_022643    
GERMANY_ERINACEINAE_2012_NC_039207    
UK_HS_2012_NC_038294    
AMERICA_WHALE_2007_NC_010646    
CHINA_BAT_2013_NC_025217    
UGANDA_BAT_2013_NC_034440    
CHINA_BAT_2006_NC_009021    
CHINA_BAT_2008_NC_010438    
CHINA_BAT_2006_NC_009020    
CHINA_BAT_2006_NC_009019    
CHINA_BAT_2006_NC_009988    
USA_BAT_2006_NC_022103    
BULGARIA_BAT_2008_NC_014470    
CHINA_BAT_2008_NC_010437    
USA_AVIAN_2004_NC_001451    
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