Strain

EUROPE_MURINE_2004_AY700211 (Region: Europe;  Strain: Murine hepatitis virus strain A59, complete genome.;  Date: 2004)

Gene

spike glycoprotein

Description

Annotated in NCBI,  spike glycoprotein

Location

AY700211(+):23930-27904

GenBank Accession

AAU06356.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGCTGTTCGTGTTTATTCTATTTTTGCCCTCTTGTTTAGGGTATATTGGTGATTTTAGATGTATCCAGCTTGTGAATTCAAACGGTGCTAATGTTAGTGCTCCAAGCATTAGCACTGAGACCGTTGAAGTTTCACAAGGCCTGGGGACATATTATGTGTTAGATCGAGTTTATTTAAATGCCACATTATTGCTTACTGGTTACTACCCGGTCGATGGTTCTAAGTTTAGAAACCTCGCTCTTACGGGAACTAACTCAGTTAGCTTGTCGTGGTTTCAACCACCCTATTTAAGTCAGTTTAATGATGGCATATTTGCGAAGGTGCAGAACCTTAAGACAAGTACGCCATCAGGTGCAACTGCATATTTTCCTACTATAGTTATAGGTAGTTTGTTTGGCTATACTTCCTATACCGTTGTAATAGAGCCATATAATGGTGTTATAATGGCCTCAGTGTGCCAGTATACCATTTGTCAGTTACCTTACACTGATTGTAAGCCTAACACTAATGGTAATAAATTAATAGGGTTTTGGCACACGGATGTAAAACCCCCAATTTGTGTGTTAAAGCGAAATTTCACGCTTAATGTTAATGCTGATGCATTTTATTTTCATTTTTACCAACATGGTGGTACTTTTTATGCGTACTATGCGGATAAACCCTCCGCTACTACGTTTTTGTTTAGTGTATATATTGGCGATATTTTAACACAGTATTATGTGTTACCTTTCATCTGCAACCCAACAGCTGGTAGCACTTTTGCTCCGCGCTATTGGGTTACACCTTTGGTTAAGCGCCAATATTTGTTTAATTTCAACCAGAAGGGTGTCATTACTAGTGCTGTTGATTGTGCTAGTAGTTATACCAGTGAAATAAAATGTAAGACCCAGAGCATGTTACCTAGCACTGGTGTCTATGAGTTATCCGGTTATACGGTCCAACCAGTTGGAGTTGTATACCGGCGTGTTGCTAACCTCCCAGCTTGTAATATAGAGGAGTGGCTTACTGCTAGGTCAGTCCCCTCCCCTCTCAACTGGGAGCGTAAGACTTTTCAGAATTGTAATTTTAATTTAAGCAGCCTGTTACGTTATGTTCAGGCTGAGAGTTTGTTTTGTAATAATATCGATGCTTCCAAAGTGTATGGCAGGTGCTTTGGTAGTATTTCAGTTGATAAGTTTGCTGTACCCCGAAGTAGGCAAGTTGATTTACAGCTTGGTAACTCTGGATTTCTGCAGACTGCTAATTATAAGATTGATACAGCTGCCACTTCGTGTCAGCTGCATTACACCTTGCCTAAGAATAATGTCACCATAAACAACCATAACCCCTCGTCTTGGAATAGGAGGTATGGCTTTAATGATGCTGGCGTCTTTGGCAAAAACCAACATGACGTTGTTTACGCTCAGCAATGTTTTACTGTAAGATCTAGTTATTGCCCGTGTGCTCAACCGGACATAGTTAGCCCTTGCACTACTCAGACTAAGCCTAAGTCTGCTTTTGTTAATGTGGGTGACCATTGTGAAGGCTTAGGTGTTTTAGAAGATAATTGTGGCAATGCTGATCCACATAAGGGTTGTATCTGTGCCAACAATTCATTTATTGGATGGTCACATGATACCTGCCTTGTTAATGATCGCTGCCAAATTTTTGCTAATATATTGTTAAATGGCATTAATAGTGGTACCACATGTTCCACAGATTTGCAGTTGCCTAATACTGAAGTGGTTACTGGCATTTGTGTCAAATATGACCTCTACGGTATTACTGGACAAGGTGTTTTTAAAGAGGTTAAGGCTGACTATTATAATAGCTGGCAAACCCTTCTGTATGATGTTAATGGTAATTTGAATGGTTTTCGTGATCTTACCACTAACAAGACTTATACGATAAGGAGCTGTTATAGTGGCCGTGTTTCTGCTGCATTTCATAAAGATGCACCCGAACCGGCTCTGCTCTATCGTAATATAAATTGTAGCTATGTTTTTAGCAATAATATTTCCCGTGAGGAGAACCCACTTAATTACTTTGATAGTTATTTGGGTTGTGTTGTTAATGCTGATAACCGCACGGATGAGGCGCTTCCTAATTGTGATCTCCGTATGGGTGCTGGCTTATGCGTTGATTATTCAAAATCACGCAGGGCTCACCGATCAGTTTCTACTGGCTATCGGTTAACTACATTTGAGCCATACACTCCGATGTTAGTTAATGATAGTGTCCAATCCGTTGATGGATTATATGAGATGCAAATACCAACCAATTTTACTATTGGGCACCATGAGGAGTTCATTCAAACTAGATCTCCAAAGGTGACTATAGATTGTGCTGCATTTGTCTGTGGTGATAACACTGCATGCAGGCAGCAGTTGGTTGAGTATGGCTCTTTCTGTGTTAATGTTAATGCCATTCTTAATGAGGTTAATAACCTCTTGGATAATATGCAACTACAAGTTGCTAGTGCATTAATGCAGGGTGTTACTATAAGCTCGAGACTGCCAGACGGCATCTCAGGCCCTATAGATGACATTAATTTTAGTCCTCTACTTGGATGCATAGGTTCAACATGTGCTGAAGACGGCAATGGACCTAGTGCAATCCGAGGGCGTTCTGCTATAGAGGATTTGTTATTTGACAAGGTCAAATTATCTGATGTTGGCTTTGTCGAGGCTTATAATAATTGCACCGGTGGTCAAGAAGTTCGTGACCTCCTTTGTGTACAATCTTTTAATGGCATCAAAGTATTACCTCCTGTGTTGTCAGAGAGTCAGATCTCTGGCTACACAACCGGTGCTACTGCGGCAGCTATGTTCCCACCGTGGTCAGCAGCTGCCGGTGTGCCATTTAGTTTAAGTGTTCAATATAGAATTAATGGTTTAGGTGTCACTATGAATGTGCTTAGTGAGAACCAAAAGATGATTGCTAGTGCTTTTAACAATGCGCTGGGTGCTATCCAGGATGGGTTTGATGCAACCAATTCTGCTTTAGGTAAGATCCAGTCCGTTGTTAATGCAAATGCTGAAGCACTCAATAACTTACTAAATCAACTTTCTAACAGGTTTGGTGCTATTAGTGCTTCTTTACAAGAAATTCTAACTCGGCTTGAGGCTGTAGAAGCAAAAGCCCAGATAGATCGTCTTATTAATGGCAGGTTAACTGCACTTAATGCGTATATATCCAAGCAACTTAGTGATAGTACGCTTATTAAAGTTAGTGCTGCTCAGGCCATAGAAAAGGTCAATGAGTGCGTTAAGAGCCAAACCACGCGTATTAATTTCTGTGGCAATGGTAATCATATATTATCTCTTGTCCAGAATGCGCCTTATGGCTTATATTTTATACACTTCAGCTATGTGCCAATATCCTTTACAACCGCAAATGTGAGTCCTGGACTTTGCATTTCTGGTGATAGAGGATTAGCACCTAAAGCTGGATATTTTGTTCAAGATGATGGAGAATGGAAGTTCACAGGCAGTTCATATTACTACCCTGAACCCATTACAGATAAAAACAGTGTCATTATGAGTAGTTGCGCAGTAAACTACACAAAGGCACCTGAAGTTTTCTTGAACACTTCAATACCTAATCCACCCGACTTTAAGGAGGAGTTAGATAAATGGTTTAAGAATCAGACGTCTATTGCGCCTGATTTATCTCTCGATTTCGAGAAGTTAAATGTTACTTTGCTGGACCTGACGTATGAGATGAACAGGATTCAGGATGCAATTAAGAAGTTAAATGAGAGCTACATCAACCTCAAGGAAGTTGGCACATATGAAATGTATGTGAAATGGCCTTGGTATGTTTGGTTGCTAATTGGATTAGCTGGTGTAGCTGTTTGTGTGTTGTTATTCTTTATATGTTGCTGCACAGGTTGTGGCTCATGTTGTTTTAAGAAGTGTGGAAATTGTTGTGATGAGTATGGAGGACACCAGGACAGTATTGTGATACATAATATTTCCTCTCATGAGGATTGA

Protein

MLFVFILFLPSCLGYIGDFRCIQLVNSNGANVSAPSISTETVEVSQGLGTYYVLDRVYLNATLLLTGYYPVDGSKFRNLALTGTNSVSLSWFQPPYLSQFNDGIFAKVQNLKTSTPSGATAYFPTIVIGSLFGYTSYTVVIEPYNGVIMASVCQYTICQLPYTDCKPNTNGNKLIGFWHTDVKPPICVLKRNFTLNVNADAFYFHFYQHGGTFYAYYADKPSATTFLFSVYIGDILTQYYVLPFICNPTAGSTFAPRYWVTPLVKRQYLFNFNQKGVITSAVDCASSYTSEIKCKTQSMLPSTGVYELSGYTVQPVGVVYRRVANLPACNIEEWLTARSVPSPLNWERKTFQNCNFNLSSLLRYVQAESLFCNNIDASKVYGRCFGSISVDKFAVPRSRQVDLQLGNSGFLQTANYKIDTAATSCQLHYTLPKNNVTINNHNPSSWNRRYGFNDAGVFGKNQHDVVYAQQCFTVRSSYCPCAQPDIVSPCTTQTKPKSAFVNVGDHCEGLGVLEDNCGNADPHKGCICANNSFIGWSHDTCLVNDRCQIFANILLNGINSGTTCSTDLQLPNTEVVTGICVKYDLYGITGQGVFKEVKADYYNSWQTLLYDVNGNLNGFRDLTTNKTYTIRSCYSGRVSAAFHKDAPEPALLYRNINCSYVFSNNISREENPLNYFDSYLGCVVNADNRTDEALPNCDLRMGAGLCVDYSKSRRAHRSVSTGYRLTTFEPYTPMLVNDSVQSVDGLYEMQIPTNFTIGHHEEFIQTRSPKVTIDCAAFVCGDNTACRQQLVEYGSFCVNVNAILNEVNNLLDNMQLQVASALMQGVTISSRLPDGISGPIDDINFSPLLGCIGSTCAEDGNGPSAIRGRSAIEDLLFDKVKLSDVGFVEAYNNCTGGQEVRDLLCVQSFNGIKVLPPVLSESQISGYTTGATAAAMFPPWSAAAGVPFSLSVQYRINGLGVTMNVLSENQKMIASAFNNALGAIQDGFDATNSALGKIQSVVNANAEALNNLLNQLSNRFGAISASLQEILTRLEAVEAKAQIDRLINGRLTALNAYISKQLSDSTLIKVSAAQAIEKVNECVKSQTTRINFCGNGNHILSLVQNAPYGLYFIHFSYVPISFTTANVSPGLCISGDRGLAPKAGYFVQDDGEWKFTGSSYYYPEPITDKNSVIMSSCAVNYTKAPEVFLNTSIPNPPDFKEELDKWFKNQTSIAPDLSLDFEKLNVTLLDLTYEMNRIQDAIKKLNESYINLKEVGTYEMYVKWPWYVWLLIGLAGVAVCVLLFFICCCTGCGSCCFKKCGNCCDEYGGHQDSIVIHNISSHED

Function

Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Interacts with murine CEACAM1 to mediate viral entry.
mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes.
Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity). Cytoplasmic tail interacts with M protein. S1 interacts with murine CEACAM1, and weakly with murine CEACAM2 in tissue culture.

Miscellaneous

The viral fusion process requires lipid rafts of the host plasma membrane (By similarity). This strain is apparently capable of entering cells either through direct fusion at the cell surface or through endocytosis.

Similarity

Belongs to the betacoronaviruses spike protein family.

Keywords

3D-structure   Coiled coil   Disulfide bond   Fusion of virus membrane with host endosomal membrane   Fusion of virus membrane with host membrane   Glycoprotein   Host cell membrane   Host membrane   Host-virus interaction   Lipoprotein   Membrane   Palmitate   Reference proteome   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Viral penetration into host cytoplasm   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike protein S2

Uniprot

C0KYR1   C0KYX9   C0KZ00   Q6RI13   S5ZBM1   C0KYV1   + More
S5YA08   P11224   Q9J3E7   C3VPE9   A0A2D1CIC0   Q6RI12   A0A2D1CLX0   Q6RI11   Q6RI10   Q9J3F1   Q83330   C0KYT1   C0KYS1   C0KYU0   P22432   C0KYY9   Q02385   C0KYX0   D4QFK1   Q83331   A7U544   C0KYW1   A0A2S0SZ25   D7PJY2   H9BZX9   F1T0N1   Q5ICX2   Q77NN1   P90210   Q9QD01   Q77NQ7   O55253   Q9IKD1   A0A096XNH1   C6GHS2   I1TMH2   I1TMG2   A0A2H4MWY0   A0A2H4MZ72   A0A096XNG8   A0A3G3NGZ3   A0A096XNI4   A0A2H4MZ10   A0A2H4MX03   A0A3G3NHC8   A0A2H4MXV6   H9AA44   A0A2H4MZ26   A4ZU74   A4ZU63   A4ZU52   I2FKH1   A0A3Q9JZN4   A0A2R4STS2   S4WLK1   A4ZU41   A0A2R4STR1   B7U2R5   B7U2N3   B7U2M2   B7U2S7   A4ZTX5   S4WIX3   E3UTE9   B7U2Q4   S4WML5   A0A3S9Y2D8   A0A451EU06   Q2EF08   E3UTE8   B9TXU9   Q2EEY7   A0A2R4STT0   A8HB16   A6YD40   Q2EF04   B7U2L1   A0A481S1B8   A0A346I7H8   A8R4D0   A0A191URZ6   A0A142D826   A0A481S1B7   A0A5J6NRR8   S4WG91   A0A5J6NUL2   C6GHL3   R4WAW7   A0A493R5L8   A0A346I7G9   Q2EEY9   A0A481S1P4   A0A346I7H0   Q2EEY6  

Pubmed

10438865    15709029    17079303    15308703    16341254    12663803    + More
2825419    9426441    10627571    1719235    8380065    8207827    15141003    15749126    16014947    15123674    11582515    21670291    8392595    7785316    2556846    1966429    9201212    12970410    1316938    20631137    17041219    20519394    21430123    15507445    9007076    11502093    10882653    25463600    30285857    22398294    17344285    23804565    18842722    17434558    20880639    17803510    16954245    17916374    17669602    30771080    17706262    26969241    31534035    23648375   

EMBL

FJ647218    ACN89689.1    FJ647225    ACN89760.1    FJ647227    ACN89777.1    + More
AY497328    AY700211    AAR92025.1    AAU06356.1    KF268336    KF268337    KF268338    AGT17716.1    AGT17727.1    AGT17738.1    FJ647222    ACN89723.1    KF268339    AGT17749.1    M18379    AF029248    AF208067    AAF69344.1    FJ884686    FJ884687    ACO72884.1    MF618252    ATN37888.1    AY497329    AAR92026.1    MF618253    ATN37896.1    AY497330    AAR92027.1    AY497331    AAR92028.1    AF208066    AAF69334.1    U14645    AAA87062.1    FJ647220    ACN89705.1    FJ647219    ACN89696.1    FJ647221    ACN89722.1    M32789    S51114    FJ647226    ACN89763.1    D10235    FJ647224    ACN89743.1    AB551247    BAJ04696.1    U14646    AAA87063.1    EF682498    ABS87264.1    FJ647223    ACN89742.1    MF416379    AWB14621.1    GU593319    ADI59790.1    JQ173883    AFD97607.1    AB593383    BAK18575.1    AY771998    AAW47240.1    AF207902    AAF68923.1    U72635    AAC56567.1    AF190406    AAF05704.1    AF201929    AAF19386.1    AB008940    BAA23719.1    AF207551    KF294372    AID16661.1    FJ938068    ACT11042.1    JF792617    AFG25760.1    JF792616    AFG25750.1    KY370043    ATP66727.1    KY370051    ATP66773.1    KF294370    AID16649.1    MH687968    AYR18599.1    KF294371    AID16655.1    KY370049    ATP66762.1    KY370048    ATP66756.1    MH687970    AYR18616.1    KY370046    ATP66744.1    JN874560    AFE48805.1    KY370044    ATP66733.1    EF424624    ABP38334.1    EF424623    ABP38328.1    EF424622    ABP38313.1    AB671299    BAM15656.1    MK095174    AZS64221.1    MH043953    AVZ61109.1    KF169923    AGO98874.1    EF424621    ABP38306.1    MH043952    AVZ61099.1    FJ425189    ACJ67000.1    FJ425186    ACJ66971.1    FJ425185    ACJ66961.1    FJ425190    ACJ67012.1    EF424615    ABP38236.1    KF169932    AGO98883.1    HM573327    HM573328    HM573329    HM573330    ADP21336.1    ADP21337.1    ADP21338.1    ADP21339.1    FJ425188    ACJ66990.1    KF169931    AGO98882.1    MK095175    AZS64222.1    MK095186    AZS64233.1    DQ389633    ABD18909.1    HM573326    ADP21335.1    EU401986    ACB30200.1    DQ389654    ABD18930.1    MH043954    MK095180    AVZ61119.1    AZS64227.1    EU019216    ABV74054.1    EF445634    ABP73656.1    DQ389637    ABD18913.1    FJ425184    ACJ66946.1    MK046009    MK046011    QBG67078.1    QBG67080.1    MH203066    MH203067    AXP11698.1    AXP11699.1    EF446615    ABP87990.1    KU886219    ANJ04974.1    KT318119    KT318120    AMQ23495.1    AMQ23496.1    MK046010    QBG67079.1    MN514971    QEY10673.1    KF169930    AGO98881.1    MN514970    QEY10672.1    FJ938063    ACT10983.1    AB775894    BAN18703.1    MK046005    MK046006    QBG67074.1    QBG67075.1    MH197037    MH197038    MH203065    AXP11688.1    AXP11689.1    AXP11697.1    DQ389652    ABD18928.1    MK046003    QBG67072.1    MH197039    AXP11690.1    DQ389655    ABD18931.1   

Proteomes

UP000151686    UP000119015    UP000138757    UP000113498    UP000112338    UP000164259    + More
UP000171340    UP000142874    UP000171975    UP000007192    UP000155907    UP000180349    UP000180350    UP000143567    UP000098534    UP000158592    UP000133456    UP000148943    UP000167944    UP000154289    UP000159025    UP000117254    UP000118088    UP000163097    UP000139707    UP000173347    UP000161770    UP000175084    UP000124585    UP000147178    UP000163913    UP000139235    UP000136116    UP000133443    UP000140344    UP000104294    UP000102696    UP000121953    UP000148210    UP000174569    UP000103372    UP000172225   

PRIDE

P11224    P22432    Q02385   

Pfam

PF09408   Spike_rec_bind
PF01601   Corona_S2
PF16451   Spike_NTD

Interpro

IPR043002   Spike_N_sf
IPR027400   S_HR2
IPR018548   Spike_rcpt-bd
IPR032500   Spike_N
IPR002552   Corona_S2
IPR042578   BETA_CORONA_SPIKE
IPR036326   Spike_rcpt-bd_sf

SUPFAM

SSF143587   SSF143587

Gene 3D

1.20.5.790     2.60.120.960    

ProteinModelPortal

C0KYR1   C0KYX9   C0KZ00   Q6RI13   S5ZBM1   C0KYV1   + More
S5YA08   P11224   Q9J3E7   C3VPE9   A0A2D1CIC0   Q6RI12   A0A2D1CLX0   Q6RI11   Q6RI10   Q9J3F1   Q83330   C0KYT1   C0KYS1   C0KYU0   P22432   C0KYY9   Q02385   C0KYX0   D4QFK1   Q83331   A7U544   C0KYW1   A0A2S0SZ25   D7PJY2   H9BZX9   F1T0N1   Q5ICX2   Q77NN1   P90210   Q9QD01   Q77NQ7   O55253   Q9IKD1   A0A096XNH1   C6GHS2   I1TMH2   I1TMG2   A0A2H4MWY0   A0A2H4MZ72   A0A096XNG8   A0A3G3NGZ3   A0A096XNI4   A0A2H4MZ10   A0A2H4MX03   A0A3G3NHC8   A0A2H4MXV6   H9AA44   A0A2H4MZ26   A4ZU74   A4ZU63   A4ZU52   I2FKH1   A0A3Q9JZN4   A0A2R4STS2   S4WLK1   A4ZU41   A0A2R4STR1   B7U2R5   B7U2N3   B7U2M2   B7U2S7   A4ZTX5   S4WIX3   E3UTE9   B7U2Q4   S4WML5   A0A3S9Y2D8   A0A451EU06   Q2EF08   E3UTE8   B9TXU9   Q2EEY7   A0A2R4STT0   A8HB16   A6YD40   Q2EF04   B7U2L1   A0A481S1B8   A0A346I7H8   A8R4D0   A0A191URZ6   A0A142D826   A0A481S1B7   A0A5J6NRR8   S4WG91   A0A5J6NUL2   C6GHL3   R4WAW7   A0A493R5L8   A0A346I7G9   Q2EEY9   A0A481S1P4   A0A346I7H0   Q2EEY6  

PDB

3JCL     E-value=0,     Score=6303