Strain

KENYA_BAT_2010_KY073745 (Region: Kenya;  Strain: NL63-related bat coronavirus strain BtKYNL63-9b, complete genome.;  Date: 2010)

Gene

S protein

Description

Annotated in NCBI,  S protein

Location

KY073745(+):20590-24711

GenBank Accession

APD51483.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGAAATTTCTGTTATTGTTATCCCTATTTTTCCCTTTGTCTTGTGCTCAGGATTTTTCATGTAATGGGCACCAAACAGACACTATGGCATTGCTACGGCTTAACTTACCACCTAACAGCACTAGTATTGTTAGTGGTTTCTTACCACTTCCTGGTGAGTCCAATTGGGCCTGTATAGCGAGTATGGACAATGAAGCCAGGTTGTTCCACAATGATTCACATGGGTTCTTTATACGTTATGTTACTTTTAACGGTCGTGTGGAACTTGGTGTGACTTCTACTCCCTATAATAGTCACACGTGGTCTATGTATTACACACGTTGGAGTAATCTTGCTTCCCTGCGTGTTTGTAGGTGGAACTCTGTCTTTAATTTAACTAATAGTGCCTCACTTGTTCTGTCTGACGATTGTTTGTTTAAACATGATTTTCATGCACCTATGCGCGAAAATTCGATTGTTGGTTTTAGTTGGTCAGGGTCACGTGTACGTTACTATGGTGAAGATGGTACTTACACATTTTATGTTCCCAATTCTTGGGACACAACTTATGTACGTTGTGATTTTCGTAAAAGTTGTGGGTTACAAGCCGTCAACTCAACCAACACATTTATTGTCACCACTGGTTCTGATGGTACTATCACTAACTATACAGTTTGTCATAATTGTTCTGGCTATTTAGGAACTATTTTTGCAACGCAACAGGGTGGCTACATACCCTCTGACTTTGACTACAACAATTGGTTTCTCTTAACCAATAGTTCATCTGTTGTAGATGGTAGGGTCAGGTCTATGCAGCCACTTCGCGTTCTTTGTCTTTGGCCCGTGCCCTCATTGTTGTCCACTTATAAGGACCTTTACTTTAATACATCTCTTAATGATGTGGAATGTAACGGCTATTCACAACATGGGCTTGCCGATGCTATACGTTTTAGTCTTAACTTCACTGAAGACCCAGCTGCTGCTTTAGCTACGGGTTCTATCATTGTGTATACTGTTGCCGGCAATACAACAATATATTGCAGCAATAGTTCTGACTCTTCTACGTATGGCATTCCCTTTGGCGCCACAGCTTTTGCCATGTATTGCTTCGCCAACACTTCTACGATTAATGCCACTAATTCCTATTTTCTTGGTGTGTTGCCCCCAAATTTGCGTGAAATTGTTTTCGCCGCCTCAGGGCAAGTTTACATCAATGGGTTTAATTACTTTACTTTGCCCCAAATTGTTGGTGTGGCATTTAACCTCACGTCTGACACTCCTGTTGATTTTTGGACTGTTGCTTTTACTACGTTCACCGATGTGTTGGTTAGTGTAAATCTCACCCAAATTCAACAAGTTGTGTACTGTGATGGTGTTGTTAACAGGCTTAAATGTTCCCAAATGGTTTTTGAATTGCCTGACGGTTTTTATTCAGCAGCTGATGTTTCTGACACTGTTTTACCTCGTTCTGTTGTAACACTCCCAACTCATTTTGACCACTCTAACGTCACTATTGATGTCACGTTTCGGTCTGGCCAACAGTGTGCTACAACTTCCCAACCACACATTTCCATCACAGCCCCACCGTGCAGTGTCAACATTACCATAGTTGACGCTGTGGATGGCGCATTTTGTGTCTCAACCAAACAATTCACCCCTCTGTTGAATGCGCATGATTTGCCAACATCTAATGGTCATACCTTCAAAGTTTATTTGGAGTCTGGTGACTGTCCTTTTTCCTTTCTTAAGTTGGCTAATTATGTTTCATTTGGTACGTTTTGTTTGGCTGATAAGCAACTTCCGGGTGGTTGTGCTATCTCTGTTGTGTCTGAGTTGCTTGGTTATAAACGTGTWATAGGTGCTGTTTATGTTTCCTATACCCATGGTGATGCTATAATCGGAGTTCCTGAAGGTGTTGTTGGTGTGCGTGAATTGTCGTCTGTTGTCTTTGACGTGTGCACATCTTACAACATCTTTGGGCGTGTGGGTACTGGTATCATTAGACTTTCTAATGACTCTTTTATAACTGGTATTTTGTATACTTCCGATTCTGGTCAATTGCTTGGTTATAAGAATGTCACTACTTCTGAGGTTTACTCAGTTACTCCTTGTCACACACCAGCACAGCTTGCTGTTTACCGTCAACGGGTTGTTGCAGCTATTTCTTCTGACAATCGAACTTATGGTTTTAATAACACCATAGCAACACCTATGTTTTACTACCACTCCGATGGTGTTAACAATTGCACGGAACCCGTGTTAGTTTACTCTAGCATTGGTGTCTGTGCTGATGGTGCCATGACTGTTGTTAAGAAGGTTGATGTCTCACCTCAAGTTGTTGCACCTATTGCCACTGCAAACATTTCTATACCAACCAACTTTACCACCTCAGTCCAGGTTGAGTATTTGCAAATAAGTCTCAAACCGGTGGTTGTTGACTGTAACACTTATGTTTGCAACGGTAATCCTAAGTGCCTTAAGCTTTTGTCTGAGTACGCTACTGCATGTAAGACTGCGGAAGAGGCCTTGCAGCTTAGTGCTAGGATGGAGGCCTCTGAAGTTAATTCAGCTATCACTTATTCTGAAGAGTCGTATCGTTTGGCTGAGAATATAACCAATGATGTTTTTAACATGTCTGCGTTGATCCCATCCGTTGTGGTTGGGGCAGGCCAAGGTTACTCAGCCAAGCGTAGTTTCGTTGAGGACCTACTCTTCGATAAGGTTGTAACATCTGGTCTTGGGACTGTTGATAAAGATTATAAAGACTGTAGTAAGGGTCTTTCTATTGCAGACCTGGCTTGTGCGCAGTATTATAATGGTATCATGGTTTTACCGGGTGTTGTCGATGCCGAACGCATGGCTATGTACACCGGTTCTTTAACCGGTGCTATGGTCATGGGCGGTGTTACAGCTGCTGCCTCCATTCCCTTTTCACTTGCTGTGCAGTCTCGCTTGAATTACGTTGCATTGCAAACTGATGTTTTGCAACGTAATCAGCAAATTCTTGCTGATTCTTTCAACAAGGCTATTGGCGGTATCACTGATGCTTTGACCAATGTTAACGATGCTTTGCTGCAGACTTCTCAGGCAATAGGTACTATTGCTGGTGCACTTAATAAAATTGAGAGTGTTGTCAATGAGCAAGGCCTTGCACTTTCTCATTTGACCAAACAACTTAAAAACAATTTTCAGGCCATCTCCTCGTCCATAGACGACATTTACAACCGCTTGGATGAGTTAGCTGCTAATGCCCAGGTTGATAGACTTATCACTGGCAGGTTGTCTGCACTTAACTCCTTTGTAGCCCAAACTCTCACTAGGTACACACAGGTACGCGCCAGTCGAGAGTTGGCGTTGCAGAAAGTTAATGAGTGTGTTAAGTCACAAACTAGCAGGTATGGCTTTTGTGGTAATGGCACCCACCTGTTTTCGATTGTCAATGCTGCGCCTGATGGCTTAATTTTCTTTCATACAGTTTTGTTGCCCACGGCTTATGTTGATGTTCAAGCTTGGTCAGGTGTCTGCATCGACGCCATGGGTTTCGTTGTCAGAGACTTTTCTCTAACTTTGGTACGCCATGACGACAAGTACTTGTTGACCCCTCGTAACATGTTTGAACCCCGCACTCCACGTGTTGAAGACTTTGTGCGAATAAATGACTGTAATGTTACGTTCCTTAACATAACAGCTGATACTTTGCCCGAAGTCTTCCCCGGCTACGTTGACGTCAATAAGACATTAGATGACTTTTTGTCATCTCTCCCCAATCGGACTCAGCCAGACTTGAGTCTTGACGTTTTTAACCAGACTTTTCTTAATTTGTCTGGCGAAATAGACAATTTAGAACTCAAGTCTCAGTCCATTCTTAACACTACCGTCAAGCTCCAGCAGCTTATTGACGAGATTAACTCAACTTTGGTAGACCTAGAGTGGCTTAACAGAGTTGAGAATTATATCAAGTGGCCGTGGTGGGTGTGGCTCATTATAGTCGTGGTTTTAATTTTTGTTGTTAGTCTGTTGGTCTTCTGTTGTATTGGTACAGGTTGTTGTGGTTGCTGCAGTTGTCTTGCTGGTTCTCTTGGCAGTTGTTGCGGTTCAACTAAACTTAAACCTTACGAGTTTGAAAAGGTTCACATTCAATAA

Protein

MKFLLLLSLFFPLSCAQDFSCNGHQTDTMALLRLNLPPNSTSIVSGFLPLPGESNWACIASMDNEARLFHNDSHGFFIRYVTFNGRVELGVTSTPYNSHTWSMYYTRWSNLASLRVCRWNSVFNLTNSASLVLSDDCLFKHDFHAPMRENSIVGFSWSGSRVRYYGEDGTYTFYVPNSWDTTYVRCDFRKSCGLQAVNSTNTFIVTTGSDGTITNYTVCHNCSGYLGTIFATQQGGYIPSDFDYNNWFLLTNSSSVVDGRVRSMQPLRVLCLWPVPSLLSTYKDLYFNTSLNDVECNGYSQHGLADAIRFSLNFTEDPAAALATGSIIVYTVAGNTTIYCSNSSDSSTYGIPFGATAFAMYCFANTSTINATNSYFLGVLPPNLREIVFAASGQVYINGFNYFTLPQIVGVAFNLTSDTPVDFWTVAFTTFTDVLVSVNLTQIQQVVYCDGVVNRLKCSQMVFELPDGFYSAADVSDTVLPRSVVTLPTHFDHSNVTIDVTFRSGQQCATTSQPHISITAPPCSVNITIVDAVDGAFCVSTKQFTPLLNAHDLPTSNGHTFKVYLESGDCPFSFLKLANYVSFGTFCLADKQLPGGCAISVVSELLGYKRVIGAVYVSYTHGDAIIGVPEGVVGVRELSSVVFDVCTSYNIFGRVGTGIIRLSNDSFITGILYTSDSGQLLGYKNVTTSEVYSVTPCHTPAQLAVYRQRVVAAISSDNRTYGFNNTIATPMFYYHSDGVNNCTEPVLVYSSIGVCADGAMTVVKKVDVSPQVVAPIATANISIPTNFTTSVQVEYLQISLKPVVVDCNTYVCNGNPKCLKLLSEYATACKTAEEALQLSARMEASEVNSAITYSEESYRLAENITNDVFNMSALIPSVVVGAGQGYSAKRSFVEDLLFDKVVTSGLGTVDKDYKDCSKGLSIADLACAQYYNGIMVLPGVVDAERMAMYTGSLTGAMVMGGVTAAASIPFSLAVQSRLNYVALQTDVLQRNQQILADSFNKAIGGITDALTNVNDALLQTSQAIGTIAGALNKIESVVNEQGLALSHLTKQLKNNFQAISSSIDDIYNRLDELAANAQVDRLITGRLSALNSFVAQTLTRYTQVRASRELALQKVNECVKSQTSRYGFCGNGTHLFSIVNAAPDGLIFFHTVLLPTAYVDVQAWSGVCIDAMGFVVRDFSLTLVRHDDKYLLTPRNMFEPRTPRVEDFVRINDCNVTFLNITADTLPEVFPGYVDVNKTLDDFLSSLPNRTQPDLSLDVFNQTFLNLSGEIDNLELKSQSILNTTVKLQQLIDEINSTLVDLEWLNRVENYIKWPWWVWLIIVVVLIFVVSLLVFCCIGTGCCGCCSCLAGSLGSCCGSTKLKPYEFEKVHIQ

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

3D-structure   Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

A0A1L2KGD3   A0A1L2KGB2   A0A1L2KGF1   A0A0P0KR98   A0A0P0KBC5   A0A0N7J128   + More
A0A0P0K0X5   A0A0P0KXK6   A0A1L2KGE4   A0A0N7J129   A0A0P0KI80   A0A0P0KMM1   A0A0P0K6L9   A0A0P0KR90   A0A1L2KGD0   A0A0P0KH07   A0A0P0KRB3   A0A0P0KWD4   A0A0U1WHD0   B1PHK2   K4JZP8   F1DAX6   K4K2R0   K4JZ69   A0A482K0W7   A0A384RK30   A0A4Y5QL66   A0A384R2Q9   H9EJ37   A0A384RBV6   A0A482JX42   A0A384RHS8   I7CMD1   H9EJW4   A0A384RHT2   A0A384QXZ7   A0A2L0WQE1   A0A513ZT30   A0A2H4ZBM8   H9EJ44   Q06Y16   K4JZD2   A0A384QZE9   A0A1L2YVI8   H9EJ51   A0A0K1D6F6   H9EJV2   H9EJ86   K4K211   A0A1V0E0W0   A0A0P0G321   H9EJA0   H9EJ72   H9EJ93   U3M6S0   A0A1V0E0U5   A0A1V0PKX1   A0A1V0PKX8   A0A5B9MUE4   U3M7H2   A0A1V0PKW6   U3M6Q4   Q6Q1S2   K4P2Y7   A0A2H4ZBN0   A0A5B9BH95   A0A1Y0EV12   A0A5B9BIJ4   A4ULL1   U3M6U5   U3M7G5   H9EJ16   Q06Y10   A0A5B9BHU7   A0A0U1WJW2   A0A482JZV2   A0A5B9BH24   A0A5B9BGI8   A0A1W5YKS6   A0A2U8JDM5   F1DB14   A0A1W5YKT3   A0A0U1UYV9   A0A482JZK4   A0A0U1WHD7   A0A0U1WHD9   A0A286R3Q7   A0A4Y6GL65   K4JZU5  

Pubmed

26378164    27125516    18420807    22933277    19239771    22561208    + More
31067830    22837419    22879617    29741740    29166910    17054987    27799635    15034574    15073334    15897467    17267506    28384506   

EMBL

KY073745    APD51483.1    KY073744    APD51475.1    KY073748    APD51507.1    + More
KT253270    ALK28781.1    KT253267    ALK28773.1    KT253261    KT253263    ALK28767.1    ALK28769.1    KT253265    ALK28771.1    KT253262    ALK28768.1    KY073747    APD51499.1    KT253269    ALK28775.1    KT253266    ALK28772.1    KT253268    ALK28774.1    KT253271    ALK28787.1    KT253264    ALK28770.1    KY073746    APD51491.1    KT253260    ALK28766.1    KT253272    ALK28793.1    KT253259    ALK28765.1    KJ473798    AIA62220.1    EU420139    ACA52171.1    JQ989266    JQ989267    AFU92070.1    AFU92078.1    HQ728480    ADX59451.1    JQ989269    AFU92095.1    JQ989268    AFU92086.1    MG916904    QBP43290.1    KM055643    AIW52840.1    MK720945    MK720946    QCX35167.1    QCX35178.1    KM055646    AIW52843.1    JQ765566    AFD98771.1    KM055638    AIW52835.1    MG916902    QBP43268.1    KM055634    AIW52831.1    JX104161    JX524171    KM055632    KM055633    KM055635    KM055636    KM055637    KM055648    KM055649    KM055650    MG428699    MG428700    MG428701    MG428702    MG428703    MG428704    MG428705    MG428706    AFO70497.1    AFQ55884.1    AIW52829.1    AIW52830.1    AIW52832.1    AIW52833.1    AIW52834.1    AIW52845.1    AIW52846.1    AIW52847.1    AWK59931.1    AWK59937.1    AWK59943.1    AWK59949.1    AWK59955.1    AWK59961.1    AWK59967.1    AWK59973.1    JQ771059    AFD64760.1    KM055644    AIW52841.1    KM055639    KM055640    KM055641    KM055645    KM055647    AIW52836.1    AIW52837.1    AIW52838.1    AIW52842.1    AIW52844.1    MG772808    AVA26873.1    MN026166    QDH43736.1    MF996621    MF996622    AUF73167.1    JQ765567    AFD98778.1    DQ445911    ABE97137.1    JQ989273    AFU92131.1    KM055642    AIW52839.1    KX179500    APF29071.1    JQ765568    AFD98785.1    KT266906    KX179494    KX179495    KX179496    AKT07952.1    APF29061.1    APF29062.1    APF29063.1    JQ771055    JQ771057    JQ771058    AFD64748.1    AFD64754.1    AFD64757.1    JQ765573    AFD98820.1    JQ989272    AFU92122.1    KY674915    KY554967    ARB07399.1    ARE29961.1    KT381875    KU521535    ALJ53431.1    ANU06084.1    JQ765575    AFD98834.1    JQ765571    JQ765572    AFD98806.1    AFD98813.1    JQ765574    AFD98827.1    KF530110    AGT51366.1    KY674916    ARB07406.1    KY554969    KY554971    KY829118    ARE29973.1    ARE29985.1    ARK08627.1    KY554970    ARE29979.1    MN306040    QEG03748.1    KF530112    AGT51380.1    KY554968    ARE29967.1    KF530105    AGT51331.1    AY567487    AY518894    AY758297    AY758298    AY758300    AY758301    JX504050    AFV53148.1    MF996623    AUF73168.1    MK334044    QED88019.1    KY983586    ARU07594.1    MK334043    QED88012.1    EF434381    ABO88151.1    KF530104    KF530106    KF530108    KF530109    KF530111    KF530113    KF530114    AGT51324.1    AGT51338.1    AGT51352.1    AGT51359.1    AGT51373.1    AGT51387.1    AGT51394.1    KF530107    AGT51345.1    JQ765563    AFD98750.1    DQ445912    JQ771056    JQ771060    JQ765564    JQ765565    JQ765569    JQ765570    ABE97130.1    AFD64751.1    AFD64763.1    AFD98757.1    AFD98764.1    AFD98792.1    AFD98799.1    MK334046    QED88033.1    KJ473807    AIA62252.1    MG916903    QBP43279.1    MK334045    QED88026.1    MK334047    QED88040.1    KY770850    ARI44789.1    MG428707    AWK59979.1    HQ728485    ADX59488.1    KY770851    ARI44794.1    KJ473799    AIA62227.1    MG916901    QBP43256.1    KJ473806    AIA62246.1    KJ473800    AIA62234.1    MF577027    ASV51733.1    MK211373    QDF43810.1    JQ989271    AFU92113.1   

Proteomes

UP000277178    UP000204102    UP000122373    UP000118770    UP000096398    UP000130985    + More
UP000110898    UP000143524    UP000126615    UP000132096    UP000119818    UP000123997    UP000108941    UP000098690    UP000158277    UP000243277    UP000125140    UP000115484    UP000126962    UP000168590    UP000141634    UP000100468    UP000112496    UP000145724    UP000110792    UP000134798    UP000141321    UP000129674    UP000143818    UP000136929    UP000171901    UP000008573    UP000161757    UP000103541    UP000106901    UP000140530    UP000144638    UP000146557    UP000153644    UP000162862    UP000167796    UP000174240    UP000173136    UP000105969    UP000130385    UP000138462    UP000151180    UP000151840    UP000106394    UP000135768    UP000144778    UP000126502    UP000162430   

Pfam

PF01601   Corona_S2
PF01600   Corona_S1

Interpro

IPR002551   Corona_S1
IPR002552   Corona_S2
IPR042552   ALPHA_CORONA_SPIKE

ProteinModelPortal

A0A1L2KGD3   A0A1L2KGB2   A0A1L2KGF1   A0A0P0KR98   A0A0P0KBC5   A0A0N7J128   + More
A0A0P0K0X5   A0A0P0KXK6   A0A1L2KGE4   A0A0N7J129   A0A0P0KI80   A0A0P0KMM1   A0A0P0K6L9   A0A0P0KR90   A0A1L2KGD0   A0A0P0KH07   A0A0P0KRB3   A0A0P0KWD4   A0A0U1WHD0   B1PHK2   K4JZP8   F1DAX6   K4K2R0   K4JZ69   A0A482K0W7   A0A384RK30   A0A4Y5QL66   A0A384R2Q9   H9EJ37   A0A384RBV6   A0A482JX42   A0A384RHS8   I7CMD1   H9EJW4   A0A384RHT2   A0A384QXZ7   A0A2L0WQE1   A0A513ZT30   A0A2H4ZBM8   H9EJ44   Q06Y16   K4JZD2   A0A384QZE9   A0A1L2YVI8   H9EJ51   A0A0K1D6F6   H9EJV2   H9EJ86   K4K211   A0A1V0E0W0   A0A0P0G321   H9EJA0   H9EJ72   H9EJ93   U3M6S0   A0A1V0E0U5   A0A1V0PKX1   A0A1V0PKX8   A0A5B9MUE4   U3M7H2   A0A1V0PKW6   U3M6Q4   Q6Q1S2   K4P2Y7   A0A2H4ZBN0   A0A5B9BH95   A0A1Y0EV12   A0A5B9BIJ4   A4ULL1   U3M6U5   U3M7G5   H9EJ16   Q06Y10   A0A5B9BHU7   A0A0U1WJW2   A0A482JZV2   A0A5B9BH24   A0A5B9BGI8   A0A1W5YKS6   A0A2U8JDM5   F1DB14   A0A1W5YKT3   A0A0U1UYV9   A0A482JZK4   A0A0U1WHD7   A0A0U1WHD9   A0A286R3Q7   A0A4Y6GL65   K4JZU5  

PDB

5SZS     E-value=0,     Score=2954