Strain

CHINA_BAT_2005_NC_009657 (Region: China;  Strain: Scotophilus bat coronavirus 512, complete genome.;  Date: 2005)

Gene

spike protein

Description

Annotated in NCBI,  spike protein

Location

NC_009657(+):20671-24786

GenBank Accession

YP_001351684.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGAAATATACACTTTTATTTTGTGTAGTTTTTGCTACGGTGTCTTTTGGCTTTGCCGATAATGAGCGCTGTAACAAAACTGTCAACCTTACTAGGTTATTTTCTAAGTTTGATATACAACCACCATCTCAAGTTGTTTTAGCTGGTCTTTTACCAAATCAGACAGCACAATGGAAGTGCACCACAGAAACTAACAAAAGAGATGAGGGTGTTGGTGTTAAGGGTGTCTTTCTTAGCTATGTGAGTTCTGGGCGTGGGTTTACGATAGGGGTTTCTCAATATAACTTTGACCCTAGTACTTACCAGCTTTACTTGCACAGAGATACAAATGGCAACTCTAATGCTTTTGCGTATTTGCGCATATGCAAGTGGCCCAGCAAGAAATGGTTACAATCTACATCCAACATGGATACCAGTGGTAGGTTTTGTTTGGTCAACAAGAAAATACCAGCTGCGTTTACTGATCATGCCAATATGGTTGTCGGTATAACATGGGACCAAGATCGGGTCACTTTTTACACTGACAAAGTCTACCATTTTTATGTCCCAAACAACCGCTGGTCACGTGTGGTGTCGTGGTGTAGTGCTGCGGATAGTTGTGCTATGCAATATATTAACAGTACTATCTATTACAATCTTAATGTAACAACACCAGGGCCTGGTGGTATCACTTATTCTGTTTGTACAAAACATTGTACTGGTTTGGCAGACAATGTTTTTTCCACAGATCAGGGTGGCCACATACCACCTATTTTTCCGTACAACAATTGGTTCTTGTTGACTAACACATCCACGTTGGTACAGGGTGTTACTCGTGTGTTTCAACCATTTCTTGTCAATTGCTTGGTTGCATTGCCTAAGTTGCAAGGTCTTACTACCACTCTGTCTTTTGACTCACCACTTAATGTGCCTGGGTTTTCCTGTAACGGCGCCAATGGTTCTAGCTCAGCGGAAGCCTTTCGTTTTAACGTCAATGATACTAAGTTGTTTGTTGGTGCTGGCGCTGTTACATTGAACACCGTCGATGGTGTTAATGTTTCTATTGTGTGCTCCAATAATGCAACACAGCCCACTAGGTCAAACAACTTGCAGGAAGACCTGCCTTACTATTGCTTCACTAACACTAGTAGCGGCACTAATCACACTGTTAAGTTTCTTTCAGTTTTCCCGCCAATCATTCGTGAGTTTGTGATCACCAAATATGGCAATGTCTATGTTAATGGCTATATCTATTTGAGAACTAGACCATTGACAGCCGTGCACTTGAACGCATCCTCTCATTCGCAGGACGTAGCAGGGTTTTGGACTATTGCCGCCACAAACTTCACGGATGTGCTTGTTGAGGTGAACAACACAGGCATTCAGAGGTTGTTGTATTGTGACACGCCTGAAAACAGTGTCAAATGTTCACAACTCTCTTTTGAACTGGAGGACGGGTTTTATTCCATGACTGCAGATAATGTTTATGCAGTAACTAAGCCCCACACGTTTGTGACTTTGCCCACGTTTAATGACCATGGGTTCGTTAATGTTACTGTGGGTGGTAACTTTGACAGTTCATACCCACCAAAGTTCACTGCTAATGGCACCTTAGTTAATAACGGCACTGTGGTGTGTGTCACTTCTAATCAGTTCACCCTTAGACACGACTTTATGGTAGGTTATTCTGCTGATATGCGTAAGGGTATATTTGAGTACTCTAGTACATGCCCTTTCAATAGAGAAACTATCAATAACTACCTTACGTTTGGTCGTATTTGTTTCTCTACTTCACCGGCGGACGGTGCTTGCGAATTGAAGTACTATGTTTGGAACACCATTGGAGCCGTTTCACACCTGGCTGGCACCTTGTATGTTCAACATACAAAGGGTGACATAATAACTGGTACACCCAAACCATTGCAGGGTTTGAATGACATTTCTGAATTGCACCTAGACACGTGCACCACTTACACCATTTATGGTTTTAGGGGTGACGGTGTTATTAGGTTGACCAATCAAACTTTCTTGTCAGGTGTCTACTACACTTCAGAGAGTGGTCAGTTATTAGCTTTTAAGAATGTCACTACAGGGCAGATTTATTCTGTTACACCCTGCCAACTGGTTCAGCAGGTTGCTTTTGTTGAGGATAGGATTGTTGGCGTCATTAGTAGTGCTAATAATACTGGGTTCTTTAATTCCACAAGAACATTTCCAGGCTTCTATTATCACTCTAATGACACCACCAATTGCACCTCACCAAGACTTGTTTACTCTAATATAGGTGTTTGTACTAGTGGTGCCATAGGTTTGCTGTCTCCTAAAGCTGCACAACCTCAGGTTCAACCCATGTTCCAGGGTAATATTAGTATCCCTACTAATTTTACTATGAGTGTGCGCACTGAGTATATACAGTTGTTTAACAAACCCGTTTCTGTAGACTGCGCAATGTATGTCTGCAATGGTAATGACCGTTGTAAGCAATTGTTGTCTCAGTACACTTCAGCATGCAAGAACATAGAATCTGCGCTGCAGCTCAGCGCAAGGTTGGAATCAATGGAGGTTAACTCTATGTTGACAGTTTCAGATGAGGCACTTAAGCTTGCCACTATAAGCCAATTTCCTGGTGGTGGTTATAATTTTACCAATATTCTTCCAGCAAATCCTGGTGCTAGGTCAGTTATTGAAGACATTTTGTTCGATAAAGTTGTCACTAGTGGTTTGGGCACAGTTGATGAAGATTATAAACGCTGCAGTAATGGACTGTCTATTGCAGATTTAGCTTGTGCGCAGCACTATAACGGCATTATGGTGTTGCCGGGTGTTGCGGACTGGGAAAAGGTCCATATGTACTCGGCTTCACTTGTCGGTGGTATGACCTTAGGTGGTATCACTTCTGCTGCGGCTTTGCCTTTCTCATATGCAGTGCAGGCAAGACTTAATTATGTTGCACTACAGACCGACGTGCTGCAACGTAATCAACAAATGCTAGCCAATTCCTTTAATAGTGCTATTAGTAACATCACATTAGCTTTTGAGAGTGTCAATAACGCTATCTATCAAACTTCTGCTGGTTTGAATACGGTAGCAGAGGCACTTTCAAAAGTACAGGATGTTGTGAATGGTCAAGGAAATGCACTCAGTCAACTAACAGTCCAATTGCAGAATAATTTTCAAGCTATTTCCAATTCTATTGGTGACATTTATAGTAGGTTAGATCAGATAACTGCTGATGCGCAAGTTGACAGACTTATCACAGGTCGGCTTGCAGCTCTTAATGCCTTTGTTGCACAGTCACTTACCAAGTATGCAGAAGTGCAAGCTAGTAGGACATTGGCCAAGCAAAAGGTTAACGAGTGTGTTAAGTCACAGTCCCCCAGATACGGTTTCTGTGGTGATGAAGGGGAACATATTTTCTCACTCACCCAAGCTGCTCCACAGGGTCTGATGTTCCTACACACCGTTTTAGTACCTAATGGTTTTATTAACGTTACAGCAGTTACAGGTTTATGTGTTGATGAGACCATAGCTATGACATTACGTCAGAGTGGATTTGTCTTGTTTGTGCAAAATGGTAATTATCTCGTGTCACCGAGGAAAATGTTTGAACCTCGGAGACCTGAAGTTGCTGATTTTGTGCAAGTAAAAACATGCACGATTAGTTATGTTAACATCACCAATAACCAGTTGCCTGACATTATTCCAGATTATGTAGACGTTAATAAGACTATAGATGAGATTTTGGCCAACCTACCTAATAATACTGTGCCTGATTTGCCACTTGATGTCTTTAATCAAACATTTCTTAATCTCACTGGTGAGATTGCAGACCTTGAAGCGCGATCTGAATCCCTTAAAAACACATCAGAAGAACTTAGACAGTTGATCCAAAATATTAACAACACACTTGTAGACCTTCAGTGGCTTAATAGGGTTGAGACCTTTATTAAGTGGCCGTGGTACGTGTGGTTGGCTATTGTTATAGCTCTTATTTTGGTTGTTTCACTGCTTGTGTTCTGCTGTATATCTACAGGTTGTTGCGGTTGTTGCGGTTGTTGTGGTTCTTGTTTCTCAGGTTGTTGTCGTGGAACTAAACTTCAACATTACGAACCAATAGAAAAGGTTCATGTGCAATAA

Protein

MKYTLLFCVVFATVSFGFADNERCNKTVNLTRLFSKFDIQPPSQVVLAGLLPNQTAQWKCTTETNKRDEGVGVKGVFLSYVSSGRGFTIGVSQYNFDPSTYQLYLHRDTNGNSNAFAYLRICKWPSKKWLQSTSNMDTSGRFCLVNKKIPAAFTDHANMVVGITWDQDRVTFYTDKVYHFYVPNNRWSRVVSWCSAADSCAMQYINSTIYYNLNVTTPGPGGITYSVCTKHCTGLADNVFSTDQGGHIPPIFPYNNWFLLTNTSTLVQGVTRVFQPFLVNCLVALPKLQGLTTTLSFDSPLNVPGFSCNGANGSSSAEAFRFNVNDTKLFVGAGAVTLNTVDGVNVSIVCSNNATQPTRSNNLQEDLPYYCFTNTSSGTNHTVKFLSVFPPIIREFVITKYGNVYVNGYIYLRTRPLTAVHLNASSHSQDVAGFWTIAATNFTDVLVEVNNTGIQRLLYCDTPENSVKCSQLSFELEDGFYSMTADNVYAVTKPHTFVTLPTFNDHGFVNVTVGGNFDSSYPPKFTANGTLVNNGTVVCVTSNQFTLRHDFMVGYSADMRKGIFEYSSTCPFNRETINNYLTFGRICFSTSPADGACELKYYVWNTIGAVSHLAGTLYVQHTKGDIITGTPKPLQGLNDISELHLDTCTTYTIYGFRGDGVIRLTNQTFLSGVYYTSESGQLLAFKNVTTGQIYSVTPCQLVQQVAFVEDRIVGVISSANNTGFFNSTRTFPGFYYHSNDTTNCTSPRLVYSNIGVCTSGAIGLLSPKAAQPQVQPMFQGNISIPTNFTMSVRTEYIQLFNKPVSVDCAMYVCNGNDRCKQLLSQYTSACKNIESALQLSARLESMEVNSMLTVSDEALKLATISQFPGGGYNFTNILPANPGARSVIEDILFDKVVTSGLGTVDEDYKRCSNGLSIADLACAQHYNGIMVLPGVADWEKVHMYSASLVGGMTLGGITSAAALPFSYAVQARLNYVALQTDVLQRNQQMLANSFNSAISNITLAFESVNNAIYQTSAGLNTVAEALSKVQDVVNGQGNALSQLTVQLQNNFQAISNSIGDIYSRLDQITADAQVDRLITGRLAALNAFVAQSLTKYAEVQASRTLAKQKVNECVKSQSPRYGFCGDEGEHIFSLTQAAPQGLMFLHTVLVPNGFINVTAVTGLCVDETIAMTLRQSGFVLFVQNGNYLVSPRKMFEPRRPEVADFVQVKTCTISYVNITNNQLPDIIPDYVDVNKTIDEILANLPNNTVPDLPLDVFNQTFLNLTGEIADLEARSESLKNTSEELRQLIQNINNTLVDLQWLNRVETFIKWPWYVWLAIVIALILVVSLLVFCCISTGCCGCCGCCGSCFSGCCRGTKLQHYEPIEKVHVQ

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Miscellaneous

Bat coronavirus 512/2005 is highly similar to porcine epidemic diarrhea virus (PEDV).

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Reference proteome   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell   3D-structure  

Feature

chain  Spike glycoprotein

Uniprot

Q0Q466   Q0Q4F6   A0A3G3NGJ7   A0A4Y6GL57   A0A4Y6GL18   A0A3G3NGM2   + More
A0A3G3NHI3   Q0Q4F5   A0A3G3NGK2   A0A219TUS3   A0A3G3NHJ0   A0A3G3NGV3   A0A3G3NGN7   A0A3G3NH05   A0A4Y6GL52   A0A3G3NGW9   A0A3G3NHL7   A0A3G3NHH0   A0A3G3NHH9   A0A3G3NGX8   A0A3G3NGM9   A0A3G3NGQ4   A0A3G3NGG7   A0A3G3NGQ0   A0A3G3NGS7   A0A3G3NGT9   I1TLC3   A0A0U3HSZ7   Q0GJJ0   A0A0M4I404   G5D078   A0A2N8Z1W1   A0A0K0WTL0   Q91AV1   A0A239QPZ3   A0A238TMM2   A0A0U1VEL1   A0A1B3Q5X6   A0A1P8A7E4   A0A238TN08   A0A0H4AC20   A0A3G2C516   A0A0M5JV19   J7LKT6   A0A5A4M1V6   A0A075ECF0   A0A109QKG8   A0A239QNQ6   A0A0F6TKU6   A0A238TQ27   A0A5A4M032   A0A0H4A7A7   A0A2N8Z212   K4G1P9   A0A238TMM6   A0A2N8Z1U7   I2EBM8   A0A5A4LZX3   A0A0M4HL41   A0A075EC92   A0A0K1LUK6   A0A2R4H2S5   A0A5A4M1T6   A0A0K1LUM5   A0A076MKN4   A0A221HZH0   S4VA59   A0A089WZ38   A0A238TM74   A0A2U8QMX8   A0A219PSP4   A0A075EAW2   A0A5A4LZS9   A0A239QPT4   A0A238TMH6   A0A2U8QMX5   A0A0M4HL64   A0A125R587   A0A0H4AE20   I3WD89   A0A5A4LZS2   A0A0K2D763   W8Q548   A0A2N8Z1T8   A0A0K2D774   A0A2R4FC33   A0A0S3MR49   A0A2H5AC28   A0A238TPF5   A0A0A8LEG6   A0A1L3KJ30   A0A1L3GVA2   A0A0A8LF79   A0A5A4M655   A0A1C7A0X8   A0A5J6KLN8   A0A1L3GVC2   A0A5A4M3B5   A0A238TMZ1  

Pubmed

16840328    22532530    27056240    17072758    26714453    21980030    + More
8291230    9782358    11724265    25279722    26044419    26477934    29981699    25217400    29540176    29770590    26841768    22840964    24750580    29243063    27880757   

EMBL

DQ648858    DQ648791    ABG11963.1    MH687941    AYR18442.1    MK211371    + More
QDF43800.1    MK211370    QDF43795.1    MH687934    AYR18400.1    MH687938    AYR18424.1    DQ648792    ABG11964.1    MH687948    AYR18481.1    KT346372    ANV78630.1    MH687954    AYR18513.1    MH687940    AYR18436.1    MH687951    AYR18499.1    MH687952    AYR18505.1    MK211369    QDF43790.1    MH687967    AYR18591.1    MH687964    AYR18573.1    MH687946    AYR18473.1    MH687950    AYR18493.1    MH687963    AYR18567.1    MH687945    AYR18467.1    MH687939    AYR18430.1    MH687936    MH687959    AYR18412.1    AYR18543.1    MH687949    AYR18487.1    MH687935    AYR18406.1    MH687962    AYR18561.1    JQ023161    AFE85962.1    KU297956    ALU34112.1    DQ862099    ABI30278.1    KT206205    ALD19741.1    JN547228    AEQ55004.1    LT906620    SNX30664.1    KP768390    AKS26489.1    AF353511    LT898439    LT900499    SNQ28038.1    SNT95659.1    LT898436    LT898435    SNQ28016.1    SNQ28045.1    KJ857477    AJD09600.1    KU985229    AOG30831.1    KU975401    AMR60838.1    LT898431    SNQ27978.1    KR296666    AKN45968.1    MG706122    AYM47397.1    KT206204    LT898418    LT898433    LT898441    ALD19740.1    SNQ27931.1    SNQ27997.1    SNQ28051.1    JX242462    AFR11480.1    MG198645    AXS76469.1    KJ645655    AID56787.1    KT428878    AMB20700.1    LT898413    LT900502    SNQ27899.1    SNT95671.1    KR011756    LT898416    LT898409    LT898410    LT898430    LT898446    LT900498    AKE47378.1    SNQ27923.1    SNQ27941.1    SNQ27961.1    SNQ27991.1    SNQ28064.1    SNT95653.1    LT898432    LT898443    SNQ27967.1    SNQ28057.1    MG198634    AXS76458.1    KR296682    AKN45984.1    LT906581    SNW64190.1    JQ979287    AFM55051.1    LT898423    SNQ27930.1    LT906582    SNW64184.1    JQ638923    AFJ97038.1    MG198640    AXS76464.1    KP399614    ALD19703.1    KJ645635    KJ645702    KJ645704    LC063846    AID56667.1    AID57069.1    AID57081.1    BAT33329.1    KR011121    AKU46226.1    MH006958    AVU05417.1    MG198635    AXS76459.1    KR011122    LT898415    LT898414    LT898411    LT898412    LT898425    LT898427    LT898426    LT898438    LT898444    LT898417    AKU46227.1    SNQ27893.1    SNQ27911.1    SNQ27917.1    SNQ27955.1    SNQ27973.1    SNQ27985.1    SNQ28010.1    SNQ28022.1    SNQ28076.1    SNQ28082.1    KJ645695    KJ645696    KM196109    KM392232    KR265759    KR265760    KM975738    KM975739    KM975740    KY619745    AID57027.1    AID57033.1    AIJ19447.1    AIN52136.1    AKJ21701.1    AKJ21707.1    AKP80610.1    AKP80617.1    AKP80624.1    ATJ03600.1    KY381581    LT898447    ASM46788.1    SNQ28088.1    KC787539    KP870120    AGO59789.1    ALA15713.1    KM403155    KM403156    KM403157    AIR95864.1    AIR95870.1    AIR95871.1    LT898421    SNQ27905.1    MH052686    AWM11504.1    KT591944    AMD11118.1    KJ645649    AID56751.1    MG198651    MG198664    AXS76475.1    AXS76488.1    LT900500    SNT95665.1    LT898440    SNQ28032.1    MH052680    AWM11503.1    KT206206    ALD19742.1    KT860508    AMD11124.1    KR296678    AKN45980.1    JQ239434    AFL02629.1    MG198641    AXS76465.1    KP870118    ALA15711.1    KJ399978    AHL38184.1    LT905450    SNU46336.1    KP870128    ALA15721.1    MF038017    AVT28076.1    LC063847    BAT33335.1    KY828991    AUG68851.1    LT898408    SNQ27887.1    LM645057    CDW77205.1    KX883635    APG77336.1    KY019624    APG31005.1    LM645058    CDW77213.1    MG198633    AXS76457.1    KM975741    AKP80631.1    MK648472    QEV88420.1    KY019623    APG30998.1    MG198659    AXS76483.1    LT898420    SNQ27949.1   

Proteomes

UP000113079    UP000122353    UP000132054    UP000095955    UP000276154    UP000008159    + More
UP000269355    UP000274124    UP000278990    UP000280596    UP000267609    UP000271078    UP000274992    UP000278554    UP000151262    UP000270497    UP000274353    UP000100204    UP000267271    UP000276298    UP000277020    UP000279022    UP000280517    UP000281047    UP000272790    UP000276964    UP000269222    UP000277354    UP000274194    UP000122852    UP000136506    UP000147669    UP000153418    UP000270191    UP000270545    UP000273819    UP000274834    UP000275549    UP000275656    UP000275831    UP000276052    UP000278039    UP000278495    UP000096695    UP000120524    UP000123161    UP000154382    UP000154704    UP000271135    UP000109281    UP000268721    UP000161506    UP000271448    UP000267306    UP000173775    UP000107011    UP000278337    UP000107825    UP000277927    UP000138211    UP000155002    UP000269867   

PRIDE

Q0Q466    Q91AV1   

Pfam

PF01600   Corona_S1
PF01601   Corona_S2

Interpro

IPR002552   Corona_S2
IPR042552   ALPHA_CORONA_SPIKE
IPR002551   Corona_S1

ProteinModelPortal

Q0Q466   Q0Q4F6   A0A3G3NGJ7   A0A4Y6GL57   A0A4Y6GL18   A0A3G3NGM2   + More
A0A3G3NHI3   Q0Q4F5   A0A3G3NGK2   A0A219TUS3   A0A3G3NHJ0   A0A3G3NGV3   A0A3G3NGN7   A0A3G3NH05   A0A4Y6GL52   A0A3G3NGW9   A0A3G3NHL7   A0A3G3NHH0   A0A3G3NHH9   A0A3G3NGX8   A0A3G3NGM9   A0A3G3NGQ4   A0A3G3NGG7   A0A3G3NGQ0   A0A3G3NGS7   A0A3G3NGT9   I1TLC3   A0A0U3HSZ7   Q0GJJ0   A0A0M4I404   G5D078   A0A2N8Z1W1   A0A0K0WTL0   Q91AV1   A0A239QPZ3   A0A238TMM2   A0A0U1VEL1   A0A1B3Q5X6   A0A1P8A7E4   A0A238TN08   A0A0H4AC20   A0A3G2C516   A0A0M5JV19   J7LKT6   A0A5A4M1V6   A0A075ECF0   A0A109QKG8   A0A239QNQ6   A0A0F6TKU6   A0A238TQ27   A0A5A4M032   A0A0H4A7A7   A0A2N8Z212   K4G1P9   A0A238TMM6   A0A2N8Z1U7   I2EBM8   A0A5A4LZX3   A0A0M4HL41   A0A075EC92   A0A0K1LUK6   A0A2R4H2S5   A0A5A4M1T6   A0A0K1LUM5   A0A076MKN4   A0A221HZH0   S4VA59   A0A089WZ38   A0A238TM74   A0A2U8QMX8   A0A219PSP4   A0A075EAW2   A0A5A4LZS9   A0A239QPT4   A0A238TMH6   A0A2U8QMX5   A0A0M4HL64   A0A125R587   A0A0H4AE20   I3WD89   A0A5A4LZS2   A0A0K2D763   W8Q548   A0A2N8Z1T8   A0A0K2D774   A0A2R4FC33   A0A0S3MR49   A0A2H5AC28   A0A238TPF5   A0A0A8LEG6   A0A1L3KJ30   A0A1L3GVA2   A0A0A8LF79   A0A5A4M655   A0A1C7A0X8   A0A5J6KLN8   A0A1L3GVC2   A0A5A4M3B5   A0A238TMZ1  

PDB

6U7K     E-value=0,     Score=4042