Strain

BULGARIA_BAT_2008_NC_014470 (Region: Bulgaria;  Strain: Bat coronavirus BM48-31/BGR/2008, complete genome.;  Date: 2008)

Gene

ORF3 protein

Description

Annotated in NCBI,  ORF3 protein

Location

NC_014470(+):25179-25994

GenBank Accession

YP_003858585.1

Full name

Protein 3a       + More
Protein 3      

Alternative Name

Accessory protein 3a
Protein U274
Protein X1
Accessory protein 3

CDS

ATGGATTTGTTTTTGAACATCTTCACTTTAGGATCTATTACTAGACAACCTGGTAAAGTTGAAAATGTTTCTCCTGCAAGTTCTTTTCATTCTACAGCGTCCATCCCTTTACAGGCCACTCTACCTTTCGGATGGCTTGTTGTTGGCGTTGCATTTCTTGCTGTTTTTCAAAGCGCTGCGAAATTAATACCTTTTAACAGTCTTTGGCAGCGTTGCTTATACCAGAGCTTTCAATTGCTTTGCAATGTGCTTCTTATTGCTTTGACAGTTTACTCGCACTTACTGCTTGTTGCTGCAGGGCTTGAAGCACCTTTCCTTTATCTACTTGCTTTGATTTACTTCTTACAGTGCGTTGTATTTGGCAGGCTTCTTGTCAGATGCTGGCTGTGCTGGAAATGCAAATCAAAGAATCCATTAATTTATGACTCAAACTATTTTGTTTGCTGGCATACTCACACTCATGACTATTGTATTCCTTACAATAGCATTACAAACACTATCGTCCTCACTGCAGGTGATGGTGTCACTATTCCCATTCGGACACAAGACTACCAAATTGGTGGTTACTTCGAAAAATGGGAATCTGGTGTTAAGGACTATCTTACACTTATTGGTCCTTTCACTGAAGTTTATTACCAGCTTGAATCTACCCAGATTTCCACAGACACTGGTATTAATAATGCGACATTCTTCCTCTTCTCAAAGAATGATGAAAGAGAACAGGAAAGTGTCCAAGTTCACACAATCGACGGCTCATCAGGAGTTGTAAACCCAATTTACGATGAGCCGACGCCGACTACTAGCGTGCCTCTTTAA

Protein

MDLFLNIFTLGSITRQPGKVENVSPASSFHSTASIPLQATLPFGWLVVGVAFLAVFQSAAKLIPFNSLWQRCLYQSFQLLCNVLLIALTVYSHLLLVAAGLEAPFLYLLALIYFLQCVVFGRLLVRCWLCWKCKSKNPLIYDSNYFVCWHTHTHDYCIPYNSITNTIVLTAGDGVTIPIRTQDYQIGGYFEKWESGVKDYLTLIGPFTEVYYQLESTQISTDTGINNATFFLFSKNDEREQESVQVHTIDGSSGVVNPIYDEPTPTTSVPL

Function

Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination.
Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (By similarity).

Subunit

Homotetramer composed of two homodimers linked non covalently. Interacts with M, S and E proteins. Also interacts with the accessory protein 7a.
Homotetramer composed of two homodimers linked non covalently. Interacts with M, S and E proteins. Also interacts with the accessory protein 7a (By similarity).

Miscellaneous

Bat coronavirus 279/2005 is highly similar to SARS-CoV (SARS-like).
Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).
Bat coronavirus HKU3 is highly similar to SARS-CoV (SARS-like).

Keywords

Apoptosis   Glycoprotein   Host cell membrane   Host cytoplasm   Host Golgi apparatus   Host membrane   Host-virus interaction   Inhibition of host innate immune response by virus   Inhibition of host interferon receptors by virus   Inhibition of host interferon signaling pathway by virus   Ion channel   Ion transport   Membrane   Reference proteome   Secreted   Transmembrane   Transmembrane helix   Transport   Viral immunoevasion   Viral ion channel   Virion  

Feature

chain  Protein 3a

Uniprot

E0XIZ4   A0A3Q8ARD5   U5WI39   U5WIM2   A0A0U2PGC2   A0A2D1PX33   + More
A0A2D1PXC3   A0A2D1PXD6   U5WIP3   A0A2D1PX58   A0A023PTR5   A0A2D1PX93   Q6TPE7   A0A2D1PX49   A0A2D1PX24   A0A0U1UZ59   A0A2D1PX91   A0A2D1PX11   A0A2D1PX81   A0A1W6S798   R9QTJ3   D2DJW5   D2E1H8   D2E2D9   Q6RD19   Q1T6X5   Q6RD63   Q6VA99   Q6VA77   Q6JH45   Q6RCY6   B8Q8Q4   Q1EFP4   Q6RD08   Q5DIC4   Q1EFN1   Q6JH37   Q6RCX5   Q3S2C4   Q6RD41   Q6R7Y5   Q6VA88   Q5Y186   Q6RCW4   Q6RCZ7   Q6RD52   Q6WGN2   J9TC74   A0A3G5BIY0   D2E236   D2E1E8   D2E266   D2E1D3   D3KDM6   P59632   D2E222   F2YKG3   Q0QDX8   Q0Q474   Q3I5J4   D2E1G3   Q6S8D6   Q692E3   Q6GYR0   Q6RD30   J9SEF0   D2E2F3   D2E2J7   C4P6Q8   B8Q8R5   B8Q8S7   A0A0U1UZ51   B8Q8W0   B8Q8U9   B8Q8T8   D5HJZ2   Q3ZTC4   Q4JDQ3   Q3ZTD9   Q4JDR6   Q3ZTF2   J9TEM7   A0A0U1UZ55   R9QTH1   D5HJS4   D5HJQ2   D5HJY1   Q3LZT6   Q3LZV2   Q3LZX0   D2DJX9   D5HK03   D5HJV9   D5HJX0   D5HJR3   D5HJT5   A0A0K1Z045   A0A0K1Z039   Q0QDY9   Q0Q483  

Pubmed

20686038    24172901    26719272    29190287    24719429    23739658    + More
20016037    14569023    20463816    14983045    16269264    14654320    16269206    18987164    16625834    14527350    14733771    16112670    17222058    19370068    30393776    22412934    12730500    12730501    12853594    12876307    15135062    15194747    15763150    15781262    15709039    16014971    15958670    16474139    16352545    16894145    20020050    16195424    16840328    15757562    20071579    16485471    16140765    16169905   

EMBL

GU190215    ADK66842.1    KY352407    APO40580.1    KC881006    AGZ48819.1    + More
KC881005    AGZ48807.1    KT444582    ALK02458.1    KY417144    KY417150    ATO98133.1    ATO98206.1    KY417151    ATO98219.1    KY417152    ATO98232.1    KF367457    AGZ48832.1    KY417146    ATO98158.1    KF569996    AHX37559.1    KY417147    ATO98170.1    AY390556    AAS00004.1    KY417145    ATO98146.1    KY417143    ATO98121.1    KJ473816    AIA62331.1    KY417149    ATO98194.1    KY417142    ATO98109.1    KY417148    ATO98182.1    KU973692    ARO76383.1    JX993988    AGC74172.1    FJ588686    ACU31034.1    FJ882929    ACZ71798.1    FJ882950    ACZ72109.1    AY502927    AAR87546.1    AB257344    BAE93402.1    AY502923    AAR87502.1    AY345986    AAP94738.1    AY345988    AAP94760.1    AY485277    AAR23245.1    AY502930    AAR87579.1    EU371559    ACB69850.1    AY864805    AAY60781.1    AY502928    AAR87557.1    AY772062    AAX16194.1    AY864806    AAY60794.1    AY485278    AAR23253.1    AY502931    AAR87590.1    DQ182595    ABA02268.1    AY502925    AAR87524.1    AY508724    AAR91587.1    AY345987    AAP94749.1    AY714217    AAU81612.1    AY502932    AAR87601.1    AY502929    AAR87568.1    AY502924    AAR87513.1    AY286320    AAR37425.1    DQ497008    DQ898174    JX163923    JX163924    JX163926    ABF65837.1    ABI96959.1    AFR58673.1    AFR58687.1    MK062179    MK062180    MK062181    MK062182    MK062183    MK062184    AYV99748.1    FJ882943    FJ882953    FJ882959    FJ882962    HQ890526    HQ890527    HQ890528    HQ890529    HQ890530    HQ890531    HQ890532    HQ890533    HQ890534    HQ890535    HQ890536    HQ890537    HQ890538    HQ890539    HQ890540    JF292902    JF292903    JF292904    JF292905    JF292906    ACZ72006.1    AEA10399.1    FJ882927    FJ882932    FJ882933    FJ882934    FJ882935    FJ882936    FJ882937    FJ882938    FJ882939    FJ882946    FJ882947    FJ882949    JF292921    KF514388    KF514392    KF514394    KF514396    KF514397    KF514398    KF514399    KF514400    KF514404    KF514408    KF514409    KF514413    KF514415    KF514418    KF514419    KF514421    KF514422    KF514423    ACZ71768.1    ACZ71842.1    AEA10999.1    AGT20794.1    AGT20854.1    AGT20884.1    AGT20914.1    AGT20929.1    AGT20944.1    AGT20959.1    AGT20974.1    AGT21034.1    AGT21094.1    AGT21109.1    AGT21169.1    AGT21199.1    AGT21244.1    AGT21259.1    AGT21289.1    AGT21304.1    AGT21319.1    FJ882945    FJ882948    FJ882952    FJ882957    FJ882958    FJ882961    HQ890541    HQ890542    HQ890543    HQ890544    HQ890545    HQ890546    JF292907    JF292908    JF292909    JF292910    JF292911    JF292912    JF292913    JF292914    JF292915    JF292916    JF292917    JF292918    JF292919    JF292920    ACZ72036.1    AEA10624.1    FJ882926    FJ882930    FJ882931    FJ882940    FJ882941    FJ882944    FJ882955    FJ882956    FJ882960    KF514389    KF514390    KF514391    KF514393    KF514395    KF514401    KF514402    KF514403    KF514405    KF514406    KF514410    KF514411    KF514412    KF514414    KF514416    KF514417    KF514420    ACZ71753.1    ACZ71827.1    ACZ71962.1    AGT20809.1    AGT20824.1    AGT20839.1    AGT20869.1    AGT20899.1    AGT20989.1    AGT21004.1    AGT21019.1    AGT21049.1    AGT21064.1    AGT21124.1    AGT21139.1    AGT21154.1    AGT21184.1    AGT21214.1    AGT21229.1    AGT21274.1    GU553363    GU553364    GU553365    JQ316196    JN854286    ADC35484.1    AFD56974.1    AFK10255.1    AY278741    AY274119    AY282752    AY278554    AY278491    AY278487    AY278488    AY278489    AY278490    AY279354    AY291451    AY310120    AY291315    AY338174    AY338175    AY348314    AP006557    AP006558    AP006559    AP006560    AP006561    AY323977    AY427439    AY463059    AY322207    FJ882942    ACZ71992.1    JF292922    JX162087    KF514407    AEA11014.1    AFM43868.1    AGT21079.1    DQ412043    ABD75326.1    DQ648857    DQ071615    FJ882928    ACZ71783.1    AY463060    AAR86776.1    AY654624    AAT76149.1    AY595412    AAT52339.1    AY502926    AAR87535.1    JX163925    JX163928    AFR58743.1    FJ882951    ACZ72123.1    FJ882954    ACZ72167.1    FJ882963    ACQ82726.1    EU371560    ACB69861.1    EU371561    ACB69873.1    KJ473814    AIA62311.1    EU371564    ACB69906.1    EU371563    ACB69895.1    EU371562    ACB69884.1    GQ153547    ADE34813.1    AY572038    AAU04665.1    AY686864    AAV49731.1    AY572035    AAU04650.1    AY686863    AAV91632.1    AY572034    AAU04635.1    JX163927    AFR58729.1    KJ473815    AIA62321.1    JX993987    AGC74166.1    GQ153541    ADE34745.1    GQ153539    ADE34723.1    GQ153546    ADE34802.1    DQ084200    AAZ41341.1    DQ084199    AAZ41330.1    DQ022305    FJ588690    ACU31049.1    GQ153548    ADE34824.1    GQ153544    ADE34780.1    GQ153545    ADE34791.1    GQ153540    ADE34734.1    GQ153542    ADE34756.1    KP886808    AKZ19077.1    KP886809    AKZ19088.1    DQ412042    ABD75316.1    DQ648856    ABG47061.1   

Proteomes

UP000149701    UP000157984    UP000138593    UP000100289    UP000110240    UP000150494    + More
UP000166164    UP000128606    UP000127630    UP000122532    UP000180365    UP000180371    UP000174857    UP000113286    UP000148533    UP000133546    UP000115935    UP000123303    UP000125082    UP000099142    UP000133349    UP000124386    UP000156013    UP000149347    UP000168969    UP000132037    UP000163176    UP000139901    UP000117876    UP000129608    UP000171372    UP000110133    UP000099158    UP000166558    UP000105036    UP000116351    UP000135071    UP000166757    UP000180362    UP000120111    UP000180410    UP000180412    UP000180413    UP000180414    UP000180415    UP000180416    UP000180417    UP000180418    UP000180419    UP000180420    UP000180421    UP000180422    UP000180423    UP000180424    UP000180425    UP000180426    UP000180427    UP000180428    UP000180429    UP000180430    UP000180431    UP000180432    UP000180433    UP000097931    UP000121467    UP000125925    UP000128288    UP000150617    UP000151673    UP000151756    UP000153830    UP000155701    UP000157930    UP000160899    UP000161442    UP000161729    UP000162782    UP000164349    UP000169779    UP000173963    UP000174280    UP000180434    UP000180435    UP000180436    UP000180437    UP000180438    UP000180439    UP000180440    UP000180441    UP000180442    UP000180443    UP000180444    UP000180445    UP000180446    UP000145008    UP000180383    UP000180384    UP000180385    UP000180386    UP000180387    UP000180388    UP000180389    UP000180390    UP000180391    UP000180393    UP000180394    UP000180395    UP000180396    UP000180397    UP000180398    UP000180399    UP000180400    UP000180401    UP000180402    UP000180403    UP000180404    UP000180405    UP000180406    UP000180407    UP000180408    UP000097852    UP000106870    UP000117796    UP000119316    UP000119820    UP000124542    UP000125968    UP000130610    UP000133348    UP000141212    UP000143912    UP000145152    UP000149516    UP000166143    UP000171773    UP000180363    UP000180366    UP000180367    UP000180368    UP000180369    UP000180370    UP000180373    UP000180374    UP000180375    UP000180377    UP000180378    UP000112510    UP000152329    UP000180379    UP000180381    UP000180382    UP000000354    UP000103670    UP000109640    UP000116947    UP000121636    UP000131569    UP000131955    UP000137377    UP000138690    UP000143093    UP000145651    UP000146108    UP000146181    UP000146296    UP000148194    UP000153467    UP000160648    UP000164441    UP000172416    UP000180409    UP000114004    UP000159535    UP000180376    UP000125672    UP000006573    UP000006570    UP000180364    UP000133987    UP000101079    UP000158790    UP000148329    UP000148108    UP000180361    UP000180411    UP000180372    UP000112237    UP000105365    UP000171533    UP000140614    UP000117687    UP000144273    UP000129563    UP000144982    UP000153024    UP000126514    UP000141980    UP000115496    UP000127950    UP000180360    UP000129833    UP000106179    UP000126363    UP000157996    UP000172322    UP000143770    UP000164299    UP000007450    UP000128853    UP000128919    UP000102617    UP000159980    UP000131329    UP000174731    UP000143681    UP000133539    UP000136409   

PRIDE

P59632   

Pfam

PF11289   APA3_viroporin

Interpro

IPR024407   SARS_coronavirus_Orf3/3a

ProteinModelPortal

E0XIZ4   A0A3Q8ARD5   U5WI39   U5WIM2   A0A0U2PGC2   A0A2D1PX33   + More
A0A2D1PXC3   A0A2D1PXD6   U5WIP3   A0A2D1PX58   A0A023PTR5   A0A2D1PX93   Q6TPE7   A0A2D1PX49   A0A2D1PX24   A0A0U1UZ59   A0A2D1PX91   A0A2D1PX11   A0A2D1PX81   A0A1W6S798   R9QTJ3   D2DJW5   D2E1H8   D2E2D9   Q6RD19   Q1T6X5   Q6RD63   Q6VA99   Q6VA77   Q6JH45   Q6RCY6   B8Q8Q4   Q1EFP4   Q6RD08   Q5DIC4   Q1EFN1   Q6JH37   Q6RCX5   Q3S2C4   Q6RD41   Q6R7Y5   Q6VA88   Q5Y186   Q6RCW4   Q6RCZ7   Q6RD52   Q6WGN2   J9TC74   A0A3G5BIY0   D2E236   D2E1E8   D2E266   D2E1D3   D3KDM6   P59632   D2E222   F2YKG3   Q0QDX8   Q0Q474   Q3I5J4   D2E1G3   Q6S8D6   Q692E3   Q6GYR0   Q6RD30   J9SEF0   D2E2F3   D2E2J7   C4P6Q8   B8Q8R5   B8Q8S7   A0A0U1UZ51   B8Q8W0   B8Q8U9   B8Q8T8   D5HJZ2   Q3ZTC4   Q4JDQ3   Q3ZTD9   Q4JDR6   Q3ZTF2   J9TEM7   A0A0U1UZ55   R9QTH1   D5HJS4   D5HJQ2   D5HJY1   Q3LZT6   Q3LZV2   Q3LZX0   D2DJX9   D5HK03   D5HJV9   D5HJX0   D5HJR3   D5HJT5   A0A0K1Z045   A0A0K1Z039   Q0QDY9   Q0Q483  

KEGG

vg:9714825     vg:1489669    

GO

GO:0016021  C:integral component of membrane
GO:0020002  C:host cell plasma membrane
GO:0051259  P:protein complex oligomerization
GO:0005576  C:extracellular region
GO:0039707  P:pore formation by virus in membrane of host cell
GO:0044178  C:host cell Golgi membrane
GO:0039502  P:suppression by virus of host type I interferon-mediated signaling pathway
GO:0019012  C:virion
GO:0039511  P:suppression by virus of host interferon receptor activity
GO:0005216  F:ion channel activity
GO:0044385  C:integral to membrane of host cell

From MSLVP

Host nucleus > host nucleolus. Host cytoplasm. Secreted.

From Uniprot

Virion  
Host Golgi apparatus membrane  
Host cell membrane  
Secreted  
Host cytoplasm  

Length:

271

Number of predicted TMHs:

3

Exp number of AAs in TMHs:

66.6259700000001

Exp number, first 60 AAs:

21.1582

Total prob of N-in:

0.00141

POSSIBLE N-term signal

sequence

outside

1  -  33

TMhelix

34  -  56

inside

57  -  76

TMhelix

77  -  99

outside

100  -  102

TMhelix

103  -  125

inside

126  -  271