Strain

CHINA_BAT_2005_NC_018871 (Region: China;  Strain: Rousettus bat coronavirus HKU10, complete genome.;  Date: 2005)

Gene

spike glycoprotein

Description

Annotated in NCBI,  spike glycoprotein

Location

NC_018871(+):20641-24690

GenBank Accession

YP_006908642.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGATTTCTCTTGCTGTTTTTATAGCAGAGATACTTGTTATTATTACGAGATTTTCGTGTCTTGCTGATCAATGTCAAGGTACACCTTGGTTAGCTAAGAACTTGAATCTTGGTTTGTCACCTAATTTGACTAATGTTTATGTACCAGGTTTGTTGCCTACAAAGGACACCACAAAGTGGTATTGTCGCACAGAATCATACTTTTTTACAGGTTATAAAGGTGTGTTTCTATCGTACCACAGTTTTGGTATGCCTTGGTCTATTGGTGTTTCCAATTCTGATCTCTCTGTTGCACGTGAAACATGGTCATTGTATGTTTCTCATGGCAATAATAGAGGTGTTATGACCTTTAGAATTTGTAAATGGCCACGTGTGCCTGGACTAGCACAAGAACCTACTCCTAACACTGGTGGTGTTAATTGTGTAGTAGATAAGCAATTTCCTTTCACTTTTCAACACGAGGCTAATGAAATGGTGGGTATTACTTGGAGTTCTGATTACGTTCGTATTTATGGGCTTCAAAGTACCTATCGTTTTTATTTTCCTAATAAGTGGAATAAAGTTAATGTTAATTGTGCACATAAGAGTTCTTGTCAGGTCTACCCTGTTAAAGACAGTGTTACATGGGTTGTTAATACCACAGCTGATGGACGTGTTAAAGATTACACTATTTGTGGTTCTTGTAATGGTTTTCCGCAACACGTCTTCCCTGTTTCACCTGGTGGTTTAATACCTCCTGATTTTAGTTTTGACGGTTGGTATGTTTTAACCAACTCTTCCACTATGGTGCAAGGTAAGTTTGTTGCATCTCAACCTTTAAAATTGTTGTGCCTTTGGCCTGTACCTGCTTTGGATTCTAGTGCTAGTGTTGTTTATTTTAATTTATCACGTAGTAGTGCGCAATGCAATGGTGCTACTGAAAATGGTTTAGCCGATGCATTACGTTTTTCTATGAATTTCTCTAGAAATGGTTTCAGTGGTGCTCACAGTTTGACTTTAAAAGGTGCCATTGATTATGTTTTTAGGTGCACTAATAACACTAACGTAACAAGTTTTTATTCTATACCATTTGGTTATATTAAAACAGTTTACAGGTGTTTTGTATCCTATAATAGCACTAATGGCACAGTGTCAGAGTTTGTGGGTGTTTTGCCACCTCTTGTTAAAGAAATTGTTGTCTCACGTTATGGCACCATTTATTTGAATGGTGTACGTATCTTTAAGTTACCGCCTTTAGAAGGTGTTGTGTTCAATGTAACTAGTGATGTTGGTTCAGACTTCTGGACTGTTGCATTTGCGCAAAACGCTGAAGTTTTGTTGGAAGTTAATGCAACTAACATTGTTAATTTGCACTATTGTGATAATACGCTGAACAAGGTTAAGTGTCAACAACTTTCTTTTCAATTACCTGATGGTTTTTATCCTACAACGAACCTGGTGGATAGTGTTGTTCCTAAAACATATGTTGCTTTACCCTATCATGCAACACACACGTACATTGAATTAAATATAACTGTTAATTATGTACGTGATGAGATAGCATTAACTGGAGGTGGTGAGTTGACTTGTGTTAATACAACACAATTCACCACACTCTTCTCAACTAATGCTGCTACTGATGGGAACACTTATGAAGGTGTAGTTATTAATGTCAATTGTCCTTTTGACTTTGACAACCTTAATAATTACCTGTCTTTTGACTCCATTTGTTTCTCTTTGAAACCAATCGCTGGTGGTTGCACTATGCGACTTGTTAAACAAATTCTTGTGTCATATTTTGACATAGGTGTTTTGTATGTTTCCTATACACCTGGTTCAAAAATTACTGGTGTTTTACAACCTACAGGTGGTGTTTATGACCCTAGTATTGTTCATATGGGTGTTTGTACAGACTACGTTGTTTATGGTCTTACTGGTAGAGGTGTTATATCTGTCAGTAACACCACTTACATTGCTGGTTTGTACTATACATCATTTGCAGGACAGTTGTTAGGTTTTAAAAATTCTACTACAGGTGTTCTTTATGCTGTTGAACCATGTGACACTTCTAGTCAAGTTGCCGTCTACGGTGATGCTATTATAGGTGCTATGACATCTTCTGCTAATGTCACTTTTGGTTTTGTTAACACCACAGTGCTACCATCTTTTTACTACCACACTAATGGTTTTGGTGGTTGTCAGACACCCGTGTTAGAGTATGGTTCCATGGGTTTGTGTGCTGACGGTAGTGTTGTAGATGTTACATTACGCCAATACCAACCAGCGCCAGTTTCACCAATTGCAACAACAAACATTAGCATACCATTAAACTTCACAATCTCCATTCAAACAGAGTACATACAGATGTACTCACAACAAGTTTCAGTTGATTGTAGTATGTATGTTTGTAATGGTAATGTTAGGTGTTTGCGTTTACTCACGCAGTATGCAACTGCTTGTAGGAATATTGAAAGTGCTTTACAACTTAGTGCTCGTTTAGAGTCGTTAGAAGTCAGCTCAGTTGTTTCTGTTTCTGAAAATGCCTTAAAGTTGGCAAATATCACACATTTTGATAGTTACAATTTATCAGTTTTGCTGCCTAAAAGTGATGGTAAAAGTGTTGTTGAAGACATACTTTTTGATAAAGTTGTTACTAGTGGTCTTGGTACCGTTGACCAGGATTACAAAAATTGTGTGGGTAAACTTGGCGTTGCACAGGACATAGCTGACGTTGGTTGTGCACAGTATTATAACGGCATCATGGTGTTACCTGGTGTTGTGAATGAAGCAAAAATGGGTTTGTATACTGCATCTTTAACAGGTGCCATGGTGATGGGAGGTTTTACAGCTGCAGCTGCTATACCATTTTCTTTGGCTGTACAATCTCGTCTTAATTATGTGGCCTTGCAAACTGATGTTCTGCAACAAAATCAAAAGTTGTTGGCAGACTCTTTTAATAATGCACTTTCTAATATAACATTGGCCTTCTCCAATGTTAATGATGCTTTGCAAGATGCTTCTGAAGCCATTAACACGGTTGCGGTGGCATTAGGCAAGGTTCAAGGTGTTGTCAATGAGCAAGGACAAGCCATAGCGCACCTTACACAACAATTAACGCAAAATTTTCAGGCTATTTCGCATTCTATAGCTGATATTTATAATAGACTTGATGAACTTGCTGCTGACGCACAAACAGACCGTTTGATAACGGGTCGTCTTAGTGCGTTGAATGCTTTTGTTACTCAAACTCTAACTAAGTCAGCTGAAGTTAAACAGAGCAGGCTTTTGGCTCAACAAAAGGTGAATGAGTGTGTTAAATCACAATCAAATCGGTTTGGTTTTTGTGGCAATGGTACACATTTGTTTTCCATTGTTAACAATGCACCTAATGGTTTGATGTTTTTCCATACCGTTTTAGTTCCTACTGTTTACGTCACAGTTTCTGCATGGGCTGGTGTTTGTTTTAATAACCGTGCGTACATTCTTAGAGATGTTGAGAGTGTGCTTTTTAATAATAACGGTTATTATGTCACTAACAGGAAGATGTATGAACCACGTGTTCCTCAGTTCTCCGACTTTGTGCAAGTTGAGAATTGTGTTGTGTCTTATTTGAATCTTTCTGATGTTGATGTTAACACTGTAATACCTGATTACATAGATGTTAACAAAACTCTAGAGGATTTGTTACAACAGTTACCAAATCACACACTTCCTGACCTTGGCCTAGATCAGTATAATAATACAATTTTAAACTTGACAGCTGAAATAGACATTTTACAGAACAAGTCTGACTTGTTGTTGGCATCTACTGAAAGGTTGCAACAACTTATTGATAACCTCAATAAGACGTATGTGGACTTAGAGTGGCTTAATAGATTTGAACAGTACATTAAGTGGCCTTGGTATGTGTGGTTGGCAATTTTGCTTGCCATCATACTATTCTCATTTTTGATGTTATATTGTTGTTGTGCCACTGGTTGTTGTGGTTGTCTTAGCTGTTTGACCAATTCTTGTGATTGTCGCGGCAAAAATTTACAACGTTACGAAGTAGAAAAAGTGCACATTCAATAA

Protein

MISLAVFIAEILVIITRFSCLADQCQGTPWLAKNLNLGLSPNLTNVYVPGLLPTKDTTKWYCRTESYFFTGYKGVFLSYHSFGMPWSIGVSNSDLSVARETWSLYVSHGNNRGVMTFRICKWPRVPGLAQEPTPNTGGVNCVVDKQFPFTFQHEANEMVGITWSSDYVRIYGLQSTYRFYFPNKWNKVNVNCAHKSSCQVYPVKDSVTWVVNTTADGRVKDYTICGSCNGFPQHVFPVSPGGLIPPDFSFDGWYVLTNSSTMVQGKFVASQPLKLLCLWPVPALDSSASVVYFNLSRSSAQCNGATENGLADALRFSMNFSRNGFSGAHSLTLKGAIDYVFRCTNNTNVTSFYSIPFGYIKTVYRCFVSYNSTNGTVSEFVGVLPPLVKEIVVSRYGTIYLNGVRIFKLPPLEGVVFNVTSDVGSDFWTVAFAQNAEVLLEVNATNIVNLHYCDNTLNKVKCQQLSFQLPDGFYPTTNLVDSVVPKTYVALPYHATHTYIELNITVNYVRDEIALTGGGELTCVNTTQFTTLFSTNAATDGNTYEGVVINVNCPFDFDNLNNYLSFDSICFSLKPIAGGCTMRLVKQILVSYFDIGVLYVSYTPGSKITGVLQPTGGVYDPSIVHMGVCTDYVVYGLTGRGVISVSNTTYIAGLYYTSFAGQLLGFKNSTTGVLYAVEPCDTSSQVAVYGDAIIGAMTSSANVTFGFVNTTVLPSFYYHTNGFGGCQTPVLEYGSMGLCADGSVVDVTLRQYQPAPVSPIATTNISIPLNFTISIQTEYIQMYSQQVSVDCSMYVCNGNVRCLRLLTQYATACRNIESALQLSARLESLEVSSVVSVSENALKLANITHFDSYNLSVLLPKSDGKSVVEDILFDKVVTSGLGTVDQDYKNCVGKLGVAQDIADVGCAQYYNGIMVLPGVVNEAKMGLYTASLTGAMVMGGFTAAAAIPFSLAVQSRLNYVALQTDVLQQNQKLLADSFNNALSNITLAFSNVNDALQDASEAINTVAVALGKVQGVVNEQGQAIAHLTQQLTQNFQAISHSIADIYNRLDELAADAQTDRLITGRLSALNAFVTQTLTKSAEVKQSRLLAQQKVNECVKSQSNRFGFCGNGTHLFSIVNNAPNGLMFFHTVLVPTVYVTVSAWAGVCFNNRAYILRDVESVLFNNNGYYVTNRKMYEPRVPQFSDFVQVENCVVSYLNLSDVDVNTVIPDYIDVNKTLEDLLQQLPNHTLPDLGLDQYNNTILNLTAEIDILQNKSDLLLASTERLQQLIDNLNKTYVDLEWLNRFEQYIKWPWYVWLAILLAIILFSFLMLYCCCATGCCGCLSCLTNSCDCRGKNLQRYEVEKVHIQ

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

3D-structure   Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

K4KCP3   K4JZU5   K4JZP8   K4K2R0   K4JZ69   K4JZD2   + More
K4K211   A0A482K0W7   A0A4Y5QL66   A0A482JX42   A0A482JZV2   A0A482JZK4   A0A4Y6GL65   A4ULL1   A0A0U1WJW2   A0A0U1UZ37   F1DAX6   A0A1L2KGD0   B1PHK2   F1DB20   A0A0U1WHD0   A0A0U1UYV9   A0A0U1WHD9   F1DB14   A0A1W5YKS6   A0A0U1WHD7   A0A0U1WHB6   A0A1W5YKT3   A0A1L2KGD3   A0A1L2KGB2   F1DB07   A0A0U1WHE4   B1PHJ5   A0A0U1WHB7   A0A1Z1W240   A0A1Z1W250   A0A1L2KGF1   A0A1Z1W238   A0A0U1WHB3   A0A1Z1W231   A0A1Z1W228   A0A0U1UZ27   A0A126KB98   A0A126KBA9   A0A3G8EWC1   A0A1Z1W243   A0A1Z1W245   A0A126K9I9   A0A126KBA2   A0A126K9J8   A0A126K9A8   A0A0P0KR98   A0A0P0KI80   A0A126KBA0   A0A0U1UZD0   A0A0U1WHD6   A0A0N7J129   A0A126K9U6   A0A0P0KBC5   A0A4Y5QL97   A0A0P0K0X5   A0A0N7J128   A0A0P0KXK6   B1PHI8   A0A1L2KGE4   A0A286R3Q7   A0A0P0KR90   A0A0P0KMM1   A0A0P0K6L9   A0A3G8EWN4   A0A0P0KWD4   A0A0P0KRB3   A0A0P0KH07   A0A3G8EWD5   A0A220YLM0   H9EJ86   A0A384R2Q9   H9EJA0   H9EJ51   H9EJ72   A0A384RHS8   A0A0P0G321   I7CMD1   A0A384RK30   A0A384QZE9   A0A384RBV6   A0A384QXZ7   U3M7G5   K4P2Y7   A0A5B9BH95   A0A384RHT2   H9EJV2   A0A1L2YVI8   H9EJ44   A0A5B9BIJ4   U3M6U5   Q6Q1S2   H9EJ37   U3M6S0   A0A5J6DE25  

Pubmed

22933277    31067830    17267506    19239771    22561208    18420807    + More
27125516    28384506    26262818    28589292    26920708    30447246    26378164    28840816    22837419    27799635    22879617    29741740    15034574    15073334    15897467    31503522   

EMBL

JQ989270    AFU92104.1    JQ989271    AFU92113.1    JQ989266    JQ989267    + More
AFU92070.1    AFU92078.1    JQ989269    AFU92095.1    JQ989268    AFU92086.1    JQ989273    AFU92131.1    JQ989272    AFU92122.1    MG916904    QBP43290.1    MK720945    MK720946    QCX35167.1    QCX35178.1    MG916902    QBP43268.1    MG916903    QBP43279.1    MG916901    QBP43256.1    MK211373    QDF43810.1    EF434381    ABO88151.1    KJ473807    AIA62252.1    KJ473810    AIA62271.1    HQ728480    ADX59451.1    KY073746    APD51491.1    EU420139    ACA52171.1    HQ728486    ADX59495.1    KJ473798    AIA62220.1    KJ473799    AIA62227.1    KJ473800    AIA62234.1    HQ728485    ADX59488.1    KY770850    ARI44789.1    KJ473806    AIA62246.1    KJ473797    AIA62212.1    KY770851    ARI44794.1    KY073745    APD51483.1    KY073744    APD51475.1    HQ728484    ADX59482.1    KJ473803    AIA62242.1    EU420138    ACA52164.1    KJ473796    AIA62206.1    KY783848    ARX80144.1    KY783851    ARX80147.1    KY073748    APD51507.1    KY783847    ARX80143.1    KJ473795    AIA62200.1    KY783846    ARX80142.1    KY783845    ARX80141.1    KJ473802    AIA62241.1    KU343203    AMB43193.1    KU343206    AMB43196.1    MH938448    AZF86118.1    KY783849    ARX80145.1    KY783850    ARX80146.1    KU343205    AMB43195.1    KU343204    AMB43194.1    KU343207    AMB43197.1    KU343208    AMB43198.1    KT253270    ALK28781.1    KT253266    ALK28772.1    KU343201    AMB43191.1    KJ473809    AIA62265.1    KJ473801    AIA62240.1    KT253269    ALK28775.1    KU343202    AMB43192.1    KT253267    ALK28773.1    MK720944    QCX35160.1    KT253265    ALK28771.1    KT253261    KT253263    ALK28767.1    ALK28769.1    KT253262    ALK28768.1    EU420137    ACA52157.1    KY073747    APD51499.1    MF577027    ASV51733.1    KT253264    ALK28770.1    KT253268    ALK28774.1    KT253271    ALK28787.1    MH938450    AZF86130.1    KT253259    ALK28765.1    KT253272    ALK28793.1    KT253260    ALK28766.1    MH938449    AZF86124.1    KY799179    ASL24654.1    JQ765573    AFD98820.1    KM055646    AIW52843.1    JQ765575    AFD98834.1    JQ765568    AFD98785.1    JQ765571    JQ765572    AFD98806.1    AFD98813.1    KM055634    AIW52831.1    KT381875    KU521535    ALJ53431.1    ANU06084.1    JX104161    JX524171    KM055632    KM055633    KM055635    KM055636    KM055637    KM055648    KM055649    KM055650    MG428699    MG428700    MG428701    MG428702    MG428703    MG428704    MG428705    MG428706    AFO70497.1    AFQ55884.1    AIW52829.1    AIW52830.1    AIW52832.1    AIW52833.1    AIW52834.1    AIW52845.1    AIW52846.1    AIW52847.1    AWK59931.1    AWK59937.1    AWK59943.1    AWK59949.1    AWK59955.1    AWK59961.1    AWK59967.1    AWK59973.1    KM055643    AIW52840.1    KM055642    AIW52839.1    KM055638    AIW52835.1    KM055639    KM055640    KM055641    KM055645    KM055647    AIW52836.1    AIW52837.1    AIW52838.1    AIW52842.1    AIW52844.1    KF530107    AGT51345.1    JX504050    AFV53148.1    MK334044    QED88019.1    KM055644    AIW52841.1    JQ771055    JQ771057    JQ771058    AFD64748.1    AFD64754.1    AFD64757.1    KX179500    APF29071.1    JQ765567    AFD98778.1    MK334043    QED88012.1    KF530104    KF530106    KF530108    KF530109    KF530111    KF530113    KF530114    AGT51324.1    AGT51338.1    AGT51352.1    AGT51359.1    AGT51373.1    AGT51387.1    AGT51394.1    AY567487    AY518894    AY758297    AY758298    AY758300    AY758301    JQ765566    AFD98771.1    KF530110    AGT51366.1    MN065811    QER90704.1   

Proteomes

UP000155419    UP000162430    UP000126615    UP000132096    UP000119818    UP000123997    + More
UP000126962    UP000112496    UP000106394    UP000144177    UP000143524    UP000110898    UP000135768    UP000126502    UP000144778    UP000106776    UP000277178    UP000204102    UP000100808    UP000157060    UP000137012    UP000122373    UP000103794    UP000118770    UP000147155    UP000096398    UP000130985    UP000100468    UP000110792    UP000168590    UP000134798    UP000141321    UP000145724    UP000098690    UP000158277    UP000174240    UP000103541    UP000125140    UP000106901    UP000140530    UP000144638    UP000146557    UP000153644    UP000162862    UP000167796    UP000008573    UP000161757    UP000108941    UP000143818   

Pfam

PF01601   Corona_S2
PF01600   Corona_S1

Interpro

IPR002552   Corona_S2
IPR002551   Corona_S1
IPR042552   ALPHA_CORONA_SPIKE

ProteinModelPortal

K4KCP3   K4JZU5   K4JZP8   K4K2R0   K4JZ69   K4JZD2   + More
K4K211   A0A482K0W7   A0A4Y5QL66   A0A482JX42   A0A482JZV2   A0A482JZK4   A0A4Y6GL65   A4ULL1   A0A0U1WJW2   A0A0U1UZ37   F1DAX6   A0A1L2KGD0   B1PHK2   F1DB20   A0A0U1WHD0   A0A0U1UYV9   A0A0U1WHD9   F1DB14   A0A1W5YKS6   A0A0U1WHD7   A0A0U1WHB6   A0A1W5YKT3   A0A1L2KGD3   A0A1L2KGB2   F1DB07   A0A0U1WHE4   B1PHJ5   A0A0U1WHB7   A0A1Z1W240   A0A1Z1W250   A0A1L2KGF1   A0A1Z1W238   A0A0U1WHB3   A0A1Z1W231   A0A1Z1W228   A0A0U1UZ27   A0A126KB98   A0A126KBA9   A0A3G8EWC1   A0A1Z1W243   A0A1Z1W245   A0A126K9I9   A0A126KBA2   A0A126K9J8   A0A126K9A8   A0A0P0KR98   A0A0P0KI80   A0A126KBA0   A0A0U1UZD0   A0A0U1WHD6   A0A0N7J129   A0A126K9U6   A0A0P0KBC5   A0A4Y5QL97   A0A0P0K0X5   A0A0N7J128   A0A0P0KXK6   B1PHI8   A0A1L2KGE4   A0A286R3Q7   A0A0P0KR90   A0A0P0KMM1   A0A0P0K6L9   A0A3G8EWN4   A0A0P0KWD4   A0A0P0KRB3   A0A0P0KH07   A0A3G8EWD5   A0A220YLM0   H9EJ86   A0A384R2Q9   H9EJA0   H9EJ51   H9EJ72   A0A384RHS8   A0A0P0G321   I7CMD1   A0A384RK30   A0A384QZE9   A0A384RBV6   A0A384QXZ7   U3M7G5   K4P2Y7   A0A5B9BH95   A0A384RHT2   H9EJV2   A0A1L2YVI8   H9EJ44   A0A5B9BIJ4   U3M6U5   Q6Q1S2   H9EJ37   U3M6S0   A0A5J6DE25  

PDB

5SZS     E-value=0,     Score=2694