Strain

USA_MURINE_1998_NC_023760 (Region: USA: Wisconsin;  Strain: Mink coronavirus strain WD1127, complete genome.;  Date: 1998)

Gene

spike protein

Description

Annotated in NCBI,  spike protein

Location

NC_023760(+):20322-24638

GenBank Accession

YP_009019182.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGTTTACTAAATTGTTGCTGCTAGCAGTTGTAAGTGTAGCAGTTAAGTGCGATGACATTCGTAATTTAACAGCACGCTGTGCCACTCTACAAAATGTAAGTATTGGGTCACATTGGTACCTTATTGACAATTTTGTTAAGAATTATAGTAGCCGCCTGCCTTCAAATTCTGACGTTGTTTTAGGCGATTATTTTCCAACTGTTAAACCGTGGTACAATTGTGTCTTCAGATATTGGGGTGCACACAATGCATTACTTTTAGAAAATCTTAAAGCACTATATTGGGACACAGTTCAAGGTGTGACAGGTGTTAATAATAAATATTTACTTGTTAGTATACATGGTTCACCGTATAGTGCTACAGTGTACCATAAAAATGGTGAGATGGCACGCTATGATAGCGGTAGATTTTGTTTGTGTAAAGGTGCCATTAATACAACTGTTGCTACAGGCTCTGGTAATTGTTGGGGCTATGATTGTAAAATTAATAGTACATTTTGGAATTGTAATAATACGGGTTGTGGTGACTTATTGTATGGTTTGCAATGGTCCAATTCAGAATTGATGGCGTACTTGTCTGGTGACATTTACCGTATTAAACTTTATAATACTTGGTTTAATAATGCGACCATTTCCAATACTTTTGCAAGTAATGGTACCACATCTAATAGCTGGTGGTTTAATCCAGTCTACAACCTTACGTATTATAGAGTTAATAAAACTGATAACACTGTTGTAGTGCAAAATTGTACCAATAATTGTGCTGACTATGTAAACAACATATTTAGTACAGAATCTGGTGGTATTATACCAGAAGGTTTCAGTTTTAACAACTGGTTTGTGTTAACTAATGATTCTACTATAACAGATGGACGCTTTTTAACACGTCAGCCATTGCTAGTTAATTGTTTATGGCCGGTTCCAAGCTTTAGTGAAACATCGCAAAACTTTTGTTTTAATACTGAAGACTTTGCGCAGTGTAATGGTTACAGACTTAATAGCACAGCTGATGTTATTAGGTTTAACCTTAATTTTACACATGAAACAGTAGCTGCCAGTGGTACAACATTTTTCGAGTTGAATACAACTGGAGGTGTTGTGCTTCATATATCATGTTACAATCAATCTAGAACAGAAGCTGTAACAAGTGAAGGTTTTTTACCATTTGGCTACCATGAGAACTCTCTTTATTGTTACGTTTCTTATAATGAAACGTTACAGAAGTTTTTGGGTGTTTTGCCACCTAGTGTTAAAGAGATTGCTATTAGTAAAGATGGTGGTTTCTATATTAATGGTTACAATTATTTCCAAACATTTCCTATTGATTGTATATCATTTAATCTAACAACTGGCAACACTGGTGCTTTCTGGACTATTGCTTATACATCATACACTGATGTTATGGTTGATGTTGAAAATACAGCCATAAAAAGAGTAGTTTATTGTAATAGTCACATTGATGATATTAGGTGTAATCAGCTTACACCCAACTTACCAGATGGATTTTACCCAGTTTCACCTAAAGCAATTGGATTTGTAAACAAGACATTTGTTACACTACCTGCTAATTTTGATCATGTGTATGTCAATATTACTGGTAATGTCAGACTTAAAATTCAGCGTGGTCGACCTTATAATAATGGTGGTAATGCAACTTTAAGTTTTCAAGGTTGTATTGACGTTTCTCAATTTACAGTCAATGTAAACCACACTTGCACACTAATTGATGCAAACGGTCAGTGTACTGGTGCTTATGAAGCCAGGGTTTATGTAACTAGTGGTAGTTGTCCATTTGCTTTCGACCGTCTTAACAACCATATGTCTTTTTCTAAAATATGTTTTTCACCTGTGCCATTGGGTGATGATTGCCGCTTAGACTTGACTGTTCAAACCCGTTATTTCACTGGTGTGTTTGCTCACGTGTATGTCAGTTATAAATTTGGTTTAGACATAGTTGGTCTTCCAAGAGCTGATGCTGGTTTGAAAGATCTCTCTGTATTGCATTTAAATGTCTGTACTGAGTACAATGTCTATGGGTTTGCTGGAACTGGTATCATACGTGAAACTAATCAAACTGTCATGGGTGGTCTATATTACACATCGCTGTCAGGTGATCTCCTAGGTTTTAAAAATGTCACAACTGGCACTGTGTACTCTATAACACCTTGTGAACTCAGTGCTCAAGCTGCAGTTATTGATGGCTCAATTGTTGGTGCTATTACGTCCGTTAATAGTGAGTTGCTAGGTTTGAAAAATCACATTGTAACACCGTATTTTTATTATTATTCAGTTTACAATTATACTGCAGATAACAACTCTAGATCCATGCAAAAGTATGATGTTAACTGCACACCTGTTATATCATATTCTAATATGGGTGTATGTGCTAATGGTGCACTTGTTTTTATTAATGTCACTCACACAAATGGTGATGTACAACCTATTAGCACTGGCAATGTCACAATCCCTAGTAATTTCACAATATCAGTTCAAATGGAGTACGTTCAAGTTTCAACTGAGCTTGTGTCTATAGATTGTGCGCGTTATGTTTGTAACGGTAATGCTAGGTGTGGTAGATTATTATCTCAGTATGTTAGTGCATGCCAGACTATTGAACAAGCACTTGCCATGGGTTCACGCTTGGAATCGATGGAACTTGAGTCTATGATATCCATTTCCGAAAATGCTTTGAAGTTGGCATCTGTTGAAGAATTTAATAGTTCTGCCATGTTAAACCCTATCTACAATGAAAGTGGTAACACTATTGGTGGTATATACTTAGATGGTTTGAAGGATATACTGCCTCGTAAGAACAAACATGGTTCTTCGCGTTCCACTATTGAAGATTTGCTATTCAACAAGGTTGTCACCACAGGGCTTGGTACTGTGGATGAAGATTACAAGCGTTGCACTAAGGGTTCTGATATTGCTGATTTAGCATGTGCACAGTATTATAATGGTATCATGGTCTTGCCGGGTGTTGCTAATGATGGTAAAATGAGCATGTATACAGCCTCTTTGTCAGGTGGCATTTCGCTTGGTGCTTTAGGTGGTGGTGCTGTCGCAATACCGTTTTCATTAGCTGTTCAGGCACGCTTAAATTATGTTGCTTTGCAGACAGATGTTTTGCAACGTAACCAGGAGATTTTAGCTGCATCATTTAATCAAGCTATTGGTAATATTACCATAGCTTTGGGTAAAGTGAATAATGCCATTTACCAAACTTCGCAGAGTTTGTCAACTGTGGCACAAGCTTTAACTAAAGTTCAAGATGTTGTTAATTCTCAAGGTAAGGCACTTAACCATCTAACACTACAATTACAAAATAATTTCCAGGCAATCAGTAGTTCTATACAAGACATCTATTATAAGTTAGATGATATTAACGCTGATGCACAAGTTGATCGTCTTATTACTGGTAGGCTGGCGGCACTTAACGCTTTTGTGACACAAACTCTCACTAGACAAGCTGAGGTTAGAGCCAGCAGACAACTTGCTAAACAGAAAGTTAATGAGTGTGTGCGTTCACAGTCCTCAAGATTTGGATTTTGTGGCAACGGTACACACTTGTTCTCTCTTGCAAATGCTGCACCTAATGGTATGGTGTTTTTCCATACAGTGTTGGTGCCCACAGCTTATCAGACAGTGACTGCTTGGTCTGGTATATGTGCTTCTGATGGTGATAGAACCTTTGGTTTGATTGTTAAGGATGTGTCTTTAACATTATTCCGTAACTATAACAACAGTTTCTATTTAACACCACGCACCATGTACCAACCACGAATCGCCACTAGTGCAGATTTTGTACAGATAGCAGATTGTGATGTCTTGTTTGTTAATGCTACTATATTAGAGCTGCCTAGTATAATACCTGATTACATTGATATTAATAAGACGGTACAGGATTTGCTAGACAGTTATAAACCTAACTGGACTGTACCAGAGTTATCATTAGACATATTCAATCAAACCTATCTTAATATTACTAATGAGATTAATGATCTGGAAAATCGATCTGTTGTTTTGTATAACACTACYAAGGAATTGGAGTTGCTAATTCAGAGTATCAACAACACACTAGTTGATCTTGAATGGCTTAACAAGATTGAAACTTATGTAAAGTGGCCTTGGTATGTGTGGGTTCTTATTGCTTTAATATTTCTTTTTGTATTACCTATGTTGTTGTTCTGTTGTTTGTCAACAGGTTGTTGTGGCTGCTGTGGATGTTTATCTAGTTGCGCAGCTGGTTGTTGTAAATATAGTTGTAGTAGAGACCTCTCGCGTTATGAACCTATTGAGAAAGTGCATGTCAATTAA

Protein

MFTKLLLLAVVSVAVKCDDIRNLTARCATLQNVSIGSHWYLIDNFVKNYSSRLPSNSDVVLGDYFPTVKPWYNCVFRYWGAHNALLLENLKALYWDTVQGVTGVNNKYLLVSIHGSPYSATVYHKNGEMARYDSGRFCLCKGAINTTVATGSGNCWGYDCKINSTFWNCNNTGCGDLLYGLQWSNSELMAYLSGDIYRIKLYNTWFNNATISNTFASNGTTSNSWWFNPVYNLTYYRVNKTDNTVVVQNCTNNCADYVNNIFSTESGGIIPEGFSFNNWFVLTNDSTITDGRFLTRQPLLVNCLWPVPSFSETSQNFCFNTEDFAQCNGYRLNSTADVIRFNLNFTHETVAASGTTFFELNTTGGVVLHISCYNQSRTEAVTSEGFLPFGYHENSLYCYVSYNETLQKFLGVLPPSVKEIAISKDGGFYINGYNYFQTFPIDCISFNLTTGNTGAFWTIAYTSYTDVMVDVENTAIKRVVYCNSHIDDIRCNQLTPNLPDGFYPVSPKAIGFVNKTFVTLPANFDHVYVNITGNVRLKIQRGRPYNNGGNATLSFQGCIDVSQFTVNVNHTCTLIDANGQCTGAYEARVYVTSGSCPFAFDRLNNHMSFSKICFSPVPLGDDCRLDLTVQTRYFTGVFAHVYVSYKFGLDIVGLPRADAGLKDLSVLHLNVCTEYNVYGFAGTGIIRETNQTVMGGLYYTSLSGDLLGFKNVTTGTVYSITPCELSAQAAVIDGSIVGAITSVNSELLGLKNHIVTPYFYYYSVYNYTADNNSRSMQKYDVNCTPVISYSNMGVCANGALVFINVTHTNGDVQPISTGNVTIPSNFTISVQMEYVQVSTELVSIDCARYVCNGNARCGRLLSQYVSACQTIEQALAMGSRLESMELESMISISENALKLASVEEFNSSAMLNPIYNESGNTIGGIYLDGLKDILPRKNKHGSSRSTIEDLLFNKVVTTGLGTVDEDYKRCTKGSDIADLACAQYYNGIMVLPGVANDGKMSMYTASLSGGISLGALGGGAVAIPFSLAVQARLNYVALQTDVLQRNQEILAASFNQAIGNITIALGKVNNAIYQTSQSLSTVAQALTKVQDVVNSQGKALNHLTLQLQNNFQAISSSIQDIYYKLDDINADAQVDRLITGRLAALNAFVTQTLTRQAEVRASRQLAKQKVNECVRSQSSRFGFCGNGTHLFSLANAAPNGMVFFHTVLVPTAYQTVTAWSGICASDGDRTFGLIVKDVSLTLFRNYNNSFYLTPRTMYQPRIATSADFVQIADCDVLFVNATILELPSIIPDYIDINKTVQDLLDSYKPNWTVPELSLDIFNQTYLNITNEINDLENRSVVLYNTTKELELLIQSINNTLVDLEWLNKIETYVKWPWYVWVLIALIFLFVLPMLLFCCLSTGCCGCCGCLSSCAAGCCKYSCSRDLSRYEPIEKVHVN

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Reference proteome   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

D9J1Z4   A0A2R4QKY5   D9J204   D3XGJ4   A0A222UBY8   A0A222UC54   + More
B7T4B7   A0A172AZS6   B7T4A8   A0A1B4X972   B7T061   E7E035   B7T072   E7E026   A4UII8   D2WXL7   A0A1L7NS37   A0A384V9H2   A0A384V9I6   Q4ZJU7   A0A224AV51   A0A384V9L2   A0A384VGG6   A0A384VGL9   A0A384VNK4   A0A384VAD1   A0A384V9T3   Q0PKZ5   A0A384V9D4   Q6PQ05   A0A384VK82   A0A1S6KDW1   Q9DY22   F1CNY7   A0A0Y0RQT7   P07946   P33470   R9RYG5   M4I7F4   A0A384VI41   A0A384V9Q2   Q0PL30   D3XGI6   P18450   Q0PL21   A0A140EDK0   A0A3T0JMA0   Q0PL04   A0A384VK31   A0A1L6BQA9   Q2LJ83   Q01977   Q7T428   A7UIZ6   A0A4Y6GTK3   C8YR35   A0A384VNP4   M4I9Z1   A0A384VA48   R4R9R9   Q84853   Q9YRA7   M4I7E9   Q88510   M4I8Z5   Q8QQW0   A0A0K2SF41   A4UII7   G8A500   Q0ZVX5   Q6SR88   I0B0S0   C6K2T6   I0B0R0   H9TEV8   A0A077JJK7   A0A1L7NS43   K7Y4M1   V9PIU7   P10033   V9PIU3   P36300   K7XKR8   F6M1Q9   K7XKS4   Q6TPL2   A0A1Z2WQT0   Q66928   S6CE14   H9TEX4   Q6T522   Q65984   S6B8Z8   Q7T6T3   Q5RZ64   O91603   S6BPG6   J7F9W6  

Pubmed

21346029    20079778    19036814    21481551    17267506    27840394    + More
17023013    22876187    3037011    2829461    1326823    10805807    2170676    1350661    8970993    1966416    25890036    1964522    20229183    10365166    8546012    22542271    16704791    17275120    25953186    22438554    20603153    25180686    23996606    3312491    16033972    10627571    1431811    8985407    22002680    8021609    15177889    18306032   

EMBL

HM245925    ADI80513.1    MF113046    AVY53336.1    HM245926    ADI80523.1    + More
GU338457    ADD49358.1    KX512810    ASR18946.1    KX512809    ASR18938.1    EU924791    ACJ64184.1    KM347965    AKG92640.1    EU924790    ACJ64175.1    LC119077    BAV31349.1    EU856361    ACJ63231.1    HQ450377    ADU17734.1    EU856362    ACJ63242.1    HQ450376    ADU17725.1    EF192156    ABO88142.1    GQ477367    ADB28907.1    LC190907    BAW32706.1    KX900399    ASV64331.1    KX900401    ASV64349.1    DQ001167    AAY22404.1    LC215871    BBA21168.1    KX900395    KX900396    ASV64295.1    ASV64304.1    KX900397    ASV64313.1    KX900405    KX900406    KX900407    ASV64384.1    ASV64393.1    ASV64402.1    KX900400    ASV64340.1    KX900403    ASV64367.1    KX900404    ASV64375.1    DQ811789    ABG89335.1    KX900394    ASV64286.1    AY587882    AAT00645.1    KX900411    ASV64438.1    KX499468    AQT01349.1    AF302263    AAG30227.1    HQ462571    JN624756    KP202848    ADY39740.1    AFA51944.1    AKA60054.1    KT696544    AMB66488.1    X05695    M21950    M94101    AJ271965    S51223    KC962433    AGM75126.1    JQ693050    AFZ88845.1    KX900398    ASV64322.1    KX900408    KX900410    ASV64411.1    ASV64429.1    DQ811785    ABG89301.1    GU338456    ADD49350.1    X53128    DQ811786    ABG89306.1    KU729220    AML22777.1    MH167923    AZV23901.1    DQ811788    ABG89325.1    KX900402    ASV64358.1    KX083668    APQ31244.1    DQ201447    ABC72414.1    M94099    AY335548    AY335549    AAQ02623.1    EU074218    ABU49659.1    MK272773    QDF46688.1    FJ755618    ACN71196.1    KX900409    ASV64420.1    JQ693052    AFZ88847.1    KX900393    ASV64277.1    KC609371    AGL80594.1    M94103    AAA46909.1    AF104420    AAC96004.1    JQ693051    AFZ88846.1    Z35758    CAA84806.1    JQ693049    AFZ88844.1    AF494337    AAM18703.1    LC029419    BAS25710.1    EF192155    ABO88141.1    GU146061    ADB10849.1    DQ112226    KP981644    AAZ91437.1    AKA65829.1    AY452031    AAR13815.2    JQ408981    AFH55121.1    GQ152141    ACS44218.1    JQ408980    AFH55111.1    JQ404409    AFG19726.1    AB907624    BAP19067.1    LC190906    BAW32705.1    KC175339    AFX81091.1    KC461235    KC461237    AGZ84516.1    AGZ84536.1    X06170    DQ010921    AY994055    KC461236    AGZ84526.1    D13096    KC175340    AFX81098.1    JF682842    AEE69322.1    KC175341    AFX81108.1    AY390342    AAQ96366.1    KY063616    KY063617    KY063618    ASB15738.1    ASB15748.1    ASB15758.1    X80799    CAA56778.1    AB781788    BAN67900.1    JQ404410    AFG19742.1    AY436637    AAR11077.1    X77047    AB781790    BAN67918.1    AY342160    AY796289    AAV65515.1    AB017789    BAA33439.1    AB781789    BAN67909.1    JN634064    AFH58021.1   

Proteomes

UP000156811    UP000132946    UP000150460    UP000272061    UP000129066    UP000158285    + More
UP000098772    UP000113525    UP000166023    UP000001440    UP000123026    UP000145148    UP000131894    UP000137024    UP000156732    UP000121388    UP000111647    UP000115330    UP000180339    UP000164750    UP000096172    UP000119169    UP000119918    UP000156095    UP000169693    UP000000835    UP000140386    UP000131699    UP000155481    UP000165378    UP000106470    UP000164655   

PRIDE

P07946    P33470    P18450    Q01977    P10033    P36300    + More
Q65984    Q7T6T3   

Pfam

PF01601   Corona_S2
PF01600   Corona_S1

Interpro

IPR002551   Corona_S1
IPR002552   Corona_S2
IPR042552   ALPHA_CORONA_SPIKE

ProteinModelPortal

D9J1Z4   A0A2R4QKY5   D9J204   D3XGJ4   A0A222UBY8   A0A222UC54   + More
B7T4B7   A0A172AZS6   B7T4A8   A0A1B4X972   B7T061   E7E035   B7T072   E7E026   A4UII8   D2WXL7   A0A1L7NS37   A0A384V9H2   A0A384V9I6   Q4ZJU7   A0A224AV51   A0A384V9L2   A0A384VGG6   A0A384VGL9   A0A384VNK4   A0A384VAD1   A0A384V9T3   Q0PKZ5   A0A384V9D4   Q6PQ05   A0A384VK82   A0A1S6KDW1   Q9DY22   F1CNY7   A0A0Y0RQT7   P07946   P33470   R9RYG5   M4I7F4   A0A384VI41   A0A384V9Q2   Q0PL30   D3XGI6   P18450   Q0PL21   A0A140EDK0   A0A3T0JMA0   Q0PL04   A0A384VK31   A0A1L6BQA9   Q2LJ83   Q01977   Q7T428   A7UIZ6   A0A4Y6GTK3   C8YR35   A0A384VNP4   M4I9Z1   A0A384VA48   R4R9R9   Q84853   Q9YRA7   M4I7E9   Q88510   M4I8Z5   Q8QQW0   A0A0K2SF41   A4UII7   G8A500   Q0ZVX5   Q6SR88   I0B0S0   C6K2T6   I0B0R0   H9TEV8   A0A077JJK7   A0A1L7NS43   K7Y4M1   V9PIU7   P10033   V9PIU3   P36300   K7XKR8   F6M1Q9   K7XKS4   Q6TPL2   A0A1Z2WQT0   Q66928   S6CE14   H9TEX4   Q6T522   Q65984   S6B8Z8   Q7T6T3   Q5RZ64   O91603   S6BPG6   J7F9W6  

PDB

6JX7     E-value=0,     Score=2643