Strain

USA_BOVIN_2001_NC_003045 (Region: USA;  Strain: Bovine coronavirus, complete genome.;  Date: 2001)

Gene

spike structural protein

Description

Annotated in NCBI,  spike structural protein

Location

NC_003045(+):23641-27732

GenBank Accession

NP_150077.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGTTTTTGATACTTTTAATTTCCTTACCAACGGCTTTTGCTGTTATAGGAGATTTAAAGTGTACTACAGTTTCCATTAATGATGTTGACACTGGTGTTCCTTCTATTAGCACTGATACTGTCGATGTTACTAATGGTTTAGGTACTTATTATGTTTTAGATCGTGTGTATTTAAATACTACGTTGTTGCTTAATGGTTACTACCCTACTTCAGGTTCTACATATCGTAATATGGCACTGAAGGGAACTTTACTATTGAGCACACTGTGGTTTAAACCACCTTTTCTTTCTGATTTTACTAATGGTATTTTTGCTAAGGTCAAAAATACCAAGGTTATTAAAGATGGTGTAATGTATAGTGAGTTTCCTGCTATAACTATAGGTAGTACTTTTGTAAATACATCTTATAGTGTGGTAGTACAACCACATACTACCATTTTGGGTAATAAATTACAAGGTTTCTTAGAGATCTCTGTTTGCCAGTATACTATGTGCGAGTACCCAAATACGATTTGTAATCCTAATCTGGGTAATCAACGCGTAGAACTATGGCATTGGGATACAGGTGTTGTTTCCTGTTTATATAAGCGTAATTTCACATACGATGTGAATGCTGATTATTTGTATTTCCATTTTTATCAAGAAGGTGGTACTTTTTATGCATATTTTACAGACACTGGTGTTGTTACTAAGTTTCTGTTTAATGTTTATTTAGGCACGGTGCTCTCACATTATTATGTCATGCCTTTGACTTGTAATAGTGCTCTGACTTTAGAATATTGGGTTACACCCCTCACTTCTAAACAATATCTACTAGCTTTCAATCAAGATGGTGTTATTTTTAATGCTGTTGATTGTAAGAGTGATTTTATGAGTGAGATTAAGTGTAAAACACTATCTATAGCACCATCTACTGGTGTTTATGAATTAAACGGTTACACTGTTCAGCCAATTGCAGATGTTTACCGACGTATACCTAATCTTCCCGATTGCAATATTGAGGCTTGGCTTAATGATAAGTCGGTGCCCTCTCCATTAAATTGGGAACGTAAGACCTTTTCAAATTGTAATTTTAATATGAGCAGCCTGATGTCTTTTATTCAGGCATACTCATTTACTTGTAATAATATTGATGCTGCTAAGATATATGGTATGTGTTTTTCCAGCATAACTATAGATAAGTTTGCAATACCCAATGGTAGGAAGGTTGACCTACAATTGGGTAATTTGGGCTATTTGCAGTCTTTTAATTATAGAATTGATACTACTGCTACAAGTTGTCAGTTGTATTATAATTTACCTGCTGCTAATGTTTCTGTTAGCAGGTTTAATCCTTCTACTTGGAATAGGAGATTTGGTTTTACAGAACAATCTGTTTTTAAGCCTCAACCTGCAGGTGTTTTTACTGATCATGATGTTGTTTATGCACAACATTGTTTTAAAGCTTCCACAAATTTCTGTCCGTGTAAATTGGATGGGTCTTTGTGTGTAGGTAATGGTCCTGGTATAGATGCTGGTTATAAAACTAGTGGCATAGGCACTTGTCCTGCAGGTACTAATTATTTAACTTGCCATAATGCTGCCCAATGTGATTGTTTGTGCACCCCAGACCCCATTACATCTAAAGCTACAGGGCCTTACAAGTGCCCCCAAACTAAATATTTAGTTGGCATAGGTGAGCACTGTTCGGGTCTTGCTATTAAAAGTGATCATTGTGGAGGTAATCCTTGTACTTGCCAACCACAAGCATTTTTGGGTTGGTCTGTTGACTCTTGTTTACAAGGGGATAGGTGTAATATTTTTGCTAATTTTATTTTGCATGATGTTAATAGTGGTACTACTTGTTCTACTGATTTACAAAAATCAAACACAGACATAATTCTTGGTGTTTGTGTTAATTATGATCTTTATGGTATTACTGGCCAAGGTATTTTTGTTGAGGTTAATGCGACTTATTATAATAGTTGGCAGAACCTTTTATATGATTCTAATGGTAATCTCTATGGTTTTAGAGACTACTTAACAAATAGAACTTTTATGATTCGTAGTTGCTATAGTGGTCGTGTTTCAGCGGCCTTTCATGCTAACTCTTCCGAACCAGCATTGCTATTTCGGAATATCAAATGCAATTACGTTTTTAATAACACTCTTTCACGACAGCTGCAACCTATTAACTATTTTGATAGCTATCTTGGTTGTGTTGTCAATGCTGATAATAGTACTTCTAGTGTTGTTCAAACATGTGATCTCACAGTAGGTAGTGGTTACTGTGTGGATTACTCTACAAAAAGACGAAGTCGTAGATCGATTACCACTGGTTATCGGTTTACTAATTTTGAGCCATTTACTGTTAATTCAGTAAATGATAGTTTAGAACCTGTAGGTGGTTTGTATGAAATTCAAATACCTTCAGAGTTTACTATAGGTAATATGGAGGAGTTTATTCAAACAAGCTCTCCTAAAGTTACTATTGATTGTTCTGCTTTTGTCTGTGGTGATTATGCAGCATGTAAATCACAGTTGGTTGAATATGGTAGCTTCTGTGACAATATTAATGCTATACTCACAGAAGTAAATGAACTACTTGACACTACACAGTTGCAAGTAGCTAATAGTTTAATGAATGGTGTCACTCTTAGCACTAAGCTTAAAGATGGCGTTAATTTCAATGTAGACGACATCAATTTTTCCCCTGTATTAGGTTGTTTAGGAAGCGATTGCAATAAAGTTTCCAGTAGATCTGCTATAGAGGACTTACTTTTTTCTAAAGTAAAGTTATCTGATGTCGGTTTCGTTGAGGCTTATAATAATTGTACTGGAGGTGCCGAAATTAGGGACCTCATTTGTGTGCAAAGTTATAATGGTATCAAGGTGTTGCCTCCACTGCTCTCGGAAAATCAGATCAGTGGATACACTTTGGCTGCCACTTCTGCTAGCCTGTTTCCTCCATGGTCAGCAGCAGCAGGTGTACCATTCTATTTAAACGTTCAGTATCGTATTAATGGTATTGGTGTTACCATGGATGTGTTAAGTCAAAATCAAAAGCTTATTGCTAATGCATTTAACAATGCTCTTGGTGCTATTCAGGAAGGGTTTGATGCTACCAATTCTGCTTTAGTTAAAATTCAAGCTGTTGTTAATGCAAATGCTGAAGCTCTTAATAACTTATTGCAACAACTCTCTAATAGATTTGGTGCTATAAGTTCTTCTTTACAAGAAATTCTATCTAGACTGGATGCTCTTGAAGCGCAAGCTCAGATAGACAGACTTATTAATGGGCGTCTTACCGCTCTTAATGCTTATGTTTCTCAACAGCTTAGTGACTCTACACTAGTAAAGTTTAGTGCAGCACAAGCTATGGAGAAGGTTAATGAATGTGTCAAAAGCCAATCATCTAGGATAAATTTTTGTGGTAATGGTAATCATATTATATCATTAGTGCAGAATGCTCCATATGGTTTGTATTTTATCCACTTTAGCTATGTCCCTACTAAGTATGTCACTGCGAAGGTTAGTCCCGGCCTGTGCATTGCTGGTGATAGAGGTATAGCCCCTAAGAGTGGTTATTTTGTTAATGTAAATAATACTTGGATGTTCACTGGTAGTGGTTATTACTACCCTGAACCTATAACTGGAAATAATGTTGTTGTTATGAGTACCTGTGCTGTTAATTATACTAAAGCGCCGGATGTAATGCTGAACATTTCAACACCCAACCTCCCTTATTTTAAGGAAGAGTTGGATCAATGGTTTAAAAACCAAACATCAGTGGCACCAGATTTGTCACTTGATTATATAAATGTTACATTCTTGGACCTACAAGATGAAATGAATAGGTTACAGGAGGCAATAAAAGTTTTAAATCAGAGCTACATCAATCTCAAGGACATTGGTACATATGAGTATTATGTAAAATGGCCTTGGTATGTATGGCTTTTAATTGGCTTTGCTGGTGTAGCTATGCTTGTTTTACTATTCTTCATATGCTGTTGTACAGGATGTGGGACTAGTTGTTTTAAGAAATGTGGTGGTTGTTGTGATGATTATACTGGACACCAGGAGTTAGTAATTAAAACATCACATGAAGACTAA

Protein

MFLILLISLPTAFAVIGDLKCTTVSINDVDTGVPSISTDTVDVTNGLGTYYVLDRVYLNTTLLLNGYYPTSGSTYRNMALKGTLLLSTLWFKPPFLSDFTNGIFAKVKNTKVIKDGVMYSEFPAITIGSTFVNTSYSVVVQPHTTILGNKLQGFLEISVCQYTMCEYPNTICNPNLGNQRVELWHWDTGVVSCLYKRNFTYDVNADYLYFHFYQEGGTFYAYFTDTGVVTKFLFNVYLGTVLSHYYVMPLTCNSALTLEYWVTPLTSKQYLLAFNQDGVIFNAVDCKSDFMSEIKCKTLSIAPSTGVYELNGYTVQPIADVYRRIPNLPDCNIEAWLNDKSVPSPLNWERKTFSNCNFNMSSLMSFIQAYSFTCNNIDAAKIYGMCFSSITIDKFAIPNGRKVDLQLGNLGYLQSFNYRIDTTATSCQLYYNLPAANVSVSRFNPSTWNRRFGFTEQSVFKPQPAGVFTDHDVVYAQHCFKASTNFCPCKLDGSLCVGNGPGIDAGYKTSGIGTCPAGTNYLTCHNAAQCDCLCTPDPITSKATGPYKCPQTKYLVGIGEHCSGLAIKSDHCGGNPCTCQPQAFLGWSVDSCLQGDRCNIFANFILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGIFVEVNATYYNSWQNLLYDSNGNLYGFRDYLTNRTFMIRSCYSGRVSAAFHANSSEPALLFRNIKCNYVFNNTLSRQLQPINYFDSYLGCVVNADNSTSSVVQTCDLTVGSGYCVDYSTKRRSRRSITTGYRFTNFEPFTVNSVNDSLEPVGGLYEIQIPSEFTIGNMEEFIQTSSPKVTIDCSAFVCGDYAACKSQLVEYGSFCDNINAILTEVNELLDTTQLQVANSLMNGVTLSTKLKDGVNFNVDDINFSPVLGCLGSDCNKVSSRSAIEDLLFSKVKLSDVGFVEAYNNCTGGAEIRDLICVQSYNGIKVLPPLLSENQISGYTLAATSASLFPPWSAAAGVPFYLNVQYRINGIGVTMDVLSQNQKLIANAFNNALGAIQEGFDATNSALVKIQAVVNANAEALNNLLQQLSNRFGAISSSLQEILSRLDALEAQAQIDRLINGRLTALNAYVSQQLSDSTLVKFSAAQAMEKVNECVKSQSSRINFCGNGNHIISLVQNAPYGLYFIHFSYVPTKYVTAKVSPGLCIAGDRGIAPKSGYFVNVNNTWMFTGSGYYYPEPITGNNVVVMSTCAVNYTKAPDVMLNISTPNLPYFKEELDQWFKNQTSVAPDLSLDYINVTFLDLQDEMNRLQEAIKVLNQSYINLKDIGTYEYYVKWPWYVWLLIGFAGVAMLVLLFFICCCTGCGTSCFKKCGGCCDDYTGHQELVIKTSHED

Function

attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes.

Similarity

Belongs to the betacoronaviruses spike protein family.

Keywords

Coiled coil   Disulfide bond   Fusion of virus membrane with host endosomal membrane   Fusion of virus membrane with host membrane   Glycoprotein   Host cell membrane   Host membrane   Host-virus interaction   Lipoprotein   Membrane   Palmitate   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Viral penetration into host cytoplasm   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

Q91A26   Q8V436   A4ZU41   A4ZTX5   A4ZU08   A4ZTY6   + More
A4ZTZ7   A0A2R4STT0   C6GHM5   C6GHN7   A4ZU30   A0A493R5L8   A0A481S1B7   B7U2R5   B7U2N3   B7U2M2   B7U2S7   A0A346I7G9   A0A2R4STR1   G8ZL44   B7U2L1   Q06BD7   A0A3Q9K3L5   B7U2Q4   A0A346I7H0   A0A346I7H6   A0A2R4STT8   A0A481S1B8   G8ZL46   A0A3S9Y2C3   B9TXU9   A0A481S1P4   A0A3Q9R3K4   C3S7Z2   A0A0C5BB53   B7U2P4   A0A3Q9K0D1   A0A3Q9R3V2   A0A3Q9K424   Q9QAR5   G8ZL45   A0A2R4STS2   A0A346I7H8   E3UTE9   A0A481S1X6   A0A451EU05   E3UTE8   A4ZU74   A4ZU63   C6GHL3   Q0PL39   A4ZU52   A0A451EU06   A0A3S9Y2J5   Q9QAQ8   Q58NS0   Q58NS1   Q2EF08   Q58NR5   Q58NS2   A6YD40   A0A142D818   Q58NR8   A0A142D822   Q58NR6   S4WLJ0   A4ZU19   Q58NR7   A0A3S9Y2S9   S4WG79   Q2EF09   Q2EEY7   Q2EF00   Q2EEY1   Q2EF04   Q58NR3   Q2EEY5   A0A3Q9JZN4   B3VTS5   A0A142D821   C6GHR0   A0A191URZ6   C6GHP9   Q2EF07   Q2EF01   A0A3Q9K3M7   Q2EF06   Q2EF02   S4WG83   S4WHI0   S4WLK4   A0A2P1IQB1   B7TYG9   A0A142D825   A0A059SQG9   A0A142D824   A0A142D831   S4WG87   A0A142D823   Q2EEY8  

Pubmed

11714968    17434558    30771080    18842722    22634277    17459444    + More
9778786    20880639    17344285    15681072    17803510    17669602    26969241    23804565    16954245    29508550    26719792   

EMBL

AF391541    AF391542    EF424621    ABP38306.1    EF424615    ABP38236.1    + More
EF424618    ABP38267.1    EF424616    ABP38243.1    EF424617    ABP38253.1    MH043954    MK095180    AVZ61119.1    AZS64227.1    FJ938064    ACT10995.1    FJ938065    ACT11007.1    EF424620    ABP38295.1    MK046005    MK046006    QBG67074.1    QBG67075.1    MK046010    QBG67079.1    FJ425189    ACJ67000.1    FJ425186    ACJ66971.1    FJ425185    ACJ66961.1    FJ425190    ACJ67012.1    MH197037    MH197038    MH203065    AXP11688.1    AXP11689.1    AXP11697.1    MH043952    AVZ61099.1    HE616738    CCE89338.1    FJ425184    ACJ66946.1    DQ915164    ABI93999.2    MK095183    AZS64230.1    FJ425188    ACJ66990.1    MH197039    AXP11690.1    MH203064    AXP11696.1    MH043955    AVZ61129.1    MK046009    MK046011    QBG67078.1    QBG67080.1    HE616740    HE616741    CCE89340.1    CCE89341.1    MK095176    AZS64223.1    EU401986    ACB30200.1    MK046003    QBG67072.1    MH741424    MH810154    MH810155    MH810156    MH810157    MH810158    MH810159    MH810160    MH810161    MH810162    AZU96230.1    AZU96315.1    AZU96316.1    AZU96317.1    AZU96318.1    AZU96319.1    AZU96320.1    AZU96321.1    AZU96322.1    AZU96323.1    EU686689    ACG75895.1    KM985631    AJL33728.1    FJ425187    ACJ66977.1    MK095181    AZS64228.1    MH810151    MH810152    MH810153    AZU96312.1    AZU96313.1    AZU96314.1    MK095182    AZS64229.1    AF058943    HE616739    CCE89339.1    MH043953    AVZ61109.1    MH203066    MH203067    AXP11698.1    AXP11699.1    HM573327    HM573328    HM573329    HM573330    ADP21336.1    ADP21337.1    ADP21338.1    ADP21339.1    MK046004    QBG67073.1    MK095179    AZS64226.1    HM573326    ADP21335.1    EF424624    ABP38334.1    EF424623    ABP38328.1    FJ938063    ACT10983.1    DQ811784    ABG89288.1    EF424622    ABP38313.1    MK095186    AZS64233.1    MK095184    AZS64231.1    AF058944    AY935639    AAX38491.1    AY935638    AAX38490.1    DQ389633    ABD18909.1    AY935644    AAX38496.1    AY935637    AAX38489.1    EF445634    ABP73656.1    KT318111    AMQ23487.1    AY935640    AY935641    AAX38492.1    AAX38493.1    KT318115    AMQ23491.1    AY935643    AAX38495.1    KF169908    AGO98859.1    EF424619    ABP38279.1    AY935642    AAX38494.1    MK095185    AZS64232.1    KF169915    AGO98866.1    DQ389632    ABD18908.1    DQ389654    ABD18930.1    DQ389641    ABD18917.1    DQ389660    ABD18936.1    DQ389637    ABD18913.1    AY935645    AY935646    AAX38497.1    AAX38498.1    DQ389656    ABD18932.1    MK095174    AZS64221.1    EU814647    ACF21935.1    KT318114    AMQ23490.1    FJ938067    ACT11030.1    KU886219    ANJ04974.1    FJ938066    ACT11019.1    DQ389634    ABD18910.1    DQ389640    ABD18916.1    MK095177    MK095178    AZS64224.1    AZS64225.1    DQ389635    ABD18911.1    DQ389639    ABD18915.1    KF169920    AGO98871.1    KF169914    AGO98865.1    KF169927    KF169928    KF169929    AGO98878.1    AGO98879.1    AGO98880.1    MG518518    AVN88332.1    FJ415324    ACJ35486.1    KT318118    AMQ23494.1    KF272919    AHA50776.1    KT318117    AMQ23493.1    KT318124    AMQ23500.1    KF169925    AGO98876.1    KT318116    AMQ23492.1    DQ389653    ABD18929.1   

Proteomes

UP000008570    UP000008571    UP000136116    UP000121953    UP000173415    UP000127099    + More
UP000151273    UP000136745    UP000139966    UP000170940    UP000133443    UP000140344    UP000104294    UP000102696    UP000174569    UP000154814    UP000148210    UP000158327    UP000147178    UP000163913    UP000172225    UP000147981    UP000139235    UP000134650    UP000164707    UP000162935    UP000158296   

PRIDE

Q91A26    Q8V436    Q9QAR5    Q9QAQ8   

Pfam

PF16451   Spike_NTD
PF01601   Corona_S2
PF09408   Spike_rec_bind

Interpro

IPR042578   BETA_CORONA_SPIKE
IPR036326   Spike_rcpt-bd_sf
IPR043002   Spike_N_sf
IPR027400   S_HR2
IPR032500   Spike_N
IPR018548   Spike_rcpt-bd
IPR002552   Corona_S2

SUPFAM

SSF143587   SSF143587

Gene 3D

1.20.5.790     2.60.120.960    

ProteinModelPortal

Q91A26   Q8V436   A4ZU41   A4ZTX5   A4ZU08   A4ZTY6   + More
A4ZTZ7   A0A2R4STT0   C6GHM5   C6GHN7   A4ZU30   A0A493R5L8   A0A481S1B7   B7U2R5   B7U2N3   B7U2M2   B7U2S7   A0A346I7G9   A0A2R4STR1   G8ZL44   B7U2L1   Q06BD7   A0A3Q9K3L5   B7U2Q4   A0A346I7H0   A0A346I7H6   A0A2R4STT8   A0A481S1B8   G8ZL46   A0A3S9Y2C3   B9TXU9   A0A481S1P4   A0A3Q9R3K4   C3S7Z2   A0A0C5BB53   B7U2P4   A0A3Q9K0D1   A0A3Q9R3V2   A0A3Q9K424   Q9QAR5   G8ZL45   A0A2R4STS2   A0A346I7H8   E3UTE9   A0A481S1X6   A0A451EU05   E3UTE8   A4ZU74   A4ZU63   C6GHL3   Q0PL39   A4ZU52   A0A451EU06   A0A3S9Y2J5   Q9QAQ8   Q58NS0   Q58NS1   Q2EF08   Q58NR5   Q58NS2   A6YD40   A0A142D818   Q58NR8   A0A142D822   Q58NR6   S4WLJ0   A4ZU19   Q58NR7   A0A3S9Y2S9   S4WG79   Q2EF09   Q2EEY7   Q2EF00   Q2EEY1   Q2EF04   Q58NR3   Q2EEY5   A0A3Q9JZN4   B3VTS5   A0A142D821   C6GHR0   A0A191URZ6   C6GHP9   Q2EF07   Q2EF01   A0A3Q9K3M7   Q2EF06   Q2EF02   S4WG83   S4WHI0   S4WLK4   A0A2P1IQB1   B7TYG9   A0A142D825   A0A059SQG9   A0A142D824   A0A142D831   S4WG87   A0A142D823   Q2EEY8  

PDB

6OHW     E-value=0,     Score=5808