Strain

GERMANY_PIG_2014_LT898427 (Region: Germany;  Strain: Porcine epidemic diarrhea virus isolate PEDV_GER_L00927-K20_14-02_2014 genome assembly, complete genome: monopartite.;  Date: 2014)

Gene

spike protein

Description

Annotated in NCBI,  spike protein

Location

LT898427(+):20634-24785

GenBank Accession

SNQ27985.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGAAGTCTTTAAATTACTTCTGGTTGTTCTTACCAGTACTTTCAACACTCAGCCTACCACAAGATGTCACTAGGTGCCAGTCCACTATTAACTTCAGGCGGTTCTTTTCAAAATTTAATGTGCAGGCACCTGCTGTCGTTGTGTTGGGTGGTTATCTACCTAGTATGAACTCCTCTAGCTGGTACTGTGGCACAGGTCTTGAAACTGCTAGTGGCGTGCATGGTATTTTCCTCAGTTACATCGATGCTGGTCAGGGCTTTGAGATTGGCATTTCACAGGAGCCGTTTGATCCTAGTGGTTACCAGCTTTATTTACATAAGGCCACTAATGGTAACCATAATGCTATTGCACGACTGCGCATTTGCCAGTTTCCAAATAATAAAACATTGGGCCCTACTGTTAATGATGTTACAACAGGTCGTAACTGCCTATTCAACAAAGCCATTCCAGCTTATATGCAGGATGGAAAAAACATCGTTGTCGGCATAACATGGGACAATGATCGTGTCACTGTTTTTGCTGACAAGATCTATCATTTTTATCTCAAAAATGATTGGTCCCGTGTTGCGACAAGATGTTACAATAAAAGAAGTTGTGCTATGCAATATGTTTATACACCTACCTACTACATGCTTAATGTTACTAGTGCAGGTGAGGATGGCATTTATTATGAACCATGTACAGCTAATTGCAGTGGTTACGCTGTCAATGTGTTTGCCACTGATTCTAATGGCCACATACCAGAAGGTTTTAGTTTTAATAATTGGTTTCTTTTGTCCAATGATTCCACTTTGTTGCATGGTAAGGTGGTTTCCAACCAACCTTTGTTGGTCAATTGTCTTTTGGCCATTCCTAAGATTTATGGACTAGGCCAATTTTTCTCATTCAATCAAACGATGGATGGCGTTTGTAATGGAGCTGCTGCGCAGCGTGCACCAGAGGCTCTGAGGTTTAATATTAATGACACCTCTGTCATTCTTGCTGAAGGCTCAATTGTACTTCACACTGCTTTAGGAACAAATCTTTCTTTTGTTTGCAGTAATTCTTCAGATCCTCATTTAGCTACCTTCACCATACCTCTGGGTGCTACCCAAGTACCCTATTATTGTTTTCTTAAAGTGGATACTTACAACTCCACTGTTTATAAATTTTTGGCTGTTTTACCTCCTACCGTCAGGGAAATTGTCATCACCAAGTATGGTGATGTTTATGTCAATGGGTTTGGATACTTGCATCTCGGTTTGTTGGATGCTGTCACAATTAATTTCACTGGTCATGGCACTGACGATGATGTTTCTGGTTTTTGGACCATAGCATCGACTAATTTTGTTGATGCACTCATCGAAGTTCAAGGAACTGCCATTCAGCGTATTCTTTATTGTGATGATCCTGTTAGCCAACTCAAGTGTTCTCAGGTTGCTTTTGACCTTGACGATGGTTTTTACCCTATTTCTTCTAGAAACCTTCTGAGTCATGAACAGCCAATTTCTTTTGTTACTCTGCCATCATTTAATGATCATTCTTTTGTTAACATTACTGTCTCTGCTTCCTTTGGTGGTCATAGTGGTGCCAACCTTATTGCATCTGACACTACTATCAATGGGTTTAGTTCTTTCTGTGTTGACACTAGACAATTTACCATTTCACTGTTTTATAACGTTACAAACAGTTATGGTTATGTGTCTAAATCACAGGACAGTAATTGCCCTTTCACCTTGCAATCTGTTAATGATTACCTGTCTTTTAGCAAATTTTGTGTTTCCACCAGCCTTTTGGCTAGTGCCTGTACCATAGATCTTTTTGGTTACCCTGAGTTTGGTAGTGGTGTTAAGTTTACGTCCCTTTACTTTCAATTCACAAAGGGTGAGTTGATTACTGGCACGCCTAAACCACTTGAAGGTGTCACGGACGTTTCTTTTATGACTCTGGATGTGTGTACCAAGTATACTATCTATGGCTTTAAAGGTGAGGGTATCATTACCCTTACAAATTCTAGCTTTTTGGCAGGTGTTTATTACACATCTGATTCTGGACAGTTGTTAGCCTTTAAGAATGTCACTAGTGGTGCTGTTTATTCTGTTACGCCATGTTCTTTTTCAGAGCAGGCTGCATATGTTGATGATGATATAGTGGGTGTTATTTCTAGTTTGTCTAGCTCCACTTTTAACAGTACTAGGGAGTTGCCTGGTTTCTTCTACCATTCTAATGATGGCTCTAATTGTACAGAGCCTGTGTTGGTGTATAGTAACATAGGTGTTTGTAAATCTGGCAGTATTGGCTACGTCCCATCTCAGTCTGGCCAAGTCAAGATTGCACCCACGGTTACTGGGAATATCAGTATTCCCACCAACTTTAGTATGAGTATTAGGACAGAATATTTACAGCTTTACAACACGCCTGTTAGTGTTGATTGTGCCACATATGTTTGTAATGGTAACTCTCGTTGTAAACAATTACTCACCCAGTACACTGCAGCATGTAAGACCATAGAGTCAGCATTACAACTCAGCGCTAGGCTTGAGTCTGTTGAAGTTAACTCTATGCTTACTATTTCTGAAGAGGCTCTACAGTTAGCTACCATTAGTTCGTTTAATGGTGATGGATATAATTTTACTAATGTGCTGGGTGTTTCTGTGTATGATCCTGCAAGTGGCAGGGTGGTACAAAAAAGGTCTTTTATTGAAGACCTGCTTTTTAATAAAGTGGTTACTAATGGCCTTGGTACTGTTGATGAAGACTATAAGCGCTGTTCTAATGGTCGCTCTGTGGCAGATCTAGTCTGTGCACAGTATTACTCTGGTGTCATGGTACTACCTGGTGTTGTTGACGCTGAGAAGCTTCACATGTATAGTGCGTCTCTCATCGGTGGTATGGTGCTAGGAGGTTTTACTTCTGCAGCGGCATTGCCTTTTAGCTATGCTGTTCAAGCTAGACTCAATTATCTTGCTCTACAGACGGATGTTCTACAGCGGAACCAGCAATTGCTTGCTGAGTCTTTTAACTCTGCTATTGGTAATATAACTTCAGCCTTTGAGAGTGTTAAAGAGGCTATTAGTCAAACTTCCAAGGGTTTGAACACTGTGGCTCATGCGCTTACTAAGGTTCAAGAGGTTGTTAACTCGCAGGGTGCAGCTTTGACTCAACTTACCGTACAGCTGCAACACAACTTCCAAGCCATTTCTAGTTCTATTGATGACATTTACTCTCGACTGGACATTCTTTCAGCCGATGTTCAGGTTGACCGTCTCATCACCGGCAGATTATCAGCACTTAATGCTTTTGTTGCTCAAACCCTCACTAAGTATACTGAGGTTCAGGCTAGCAGGAAGCTAGCACAGCAAAAGGTTAATGAGTGCGTTAAATCGCAATCTCAGCGTTATGGTTTTTGTGGTGGTGATGGCGAGCACATTTTCTCTCTGGTACAGGCAGCACCTCAGGGCCTGCTGTTTTTACATACAGTACTTGTACCGGGTGATTTTATAGATGTTATTGCCATCGCTGGCTTATGCGTTAACGATGAAATTGCCTTGACTCTACGTGAGCCTGGCTTAGTCTTGTTTACGCATGAACTTCAAAATCATACTGCGACGGAATATTTTGTTTCATCGCGACGTATGTTTGAACCTAGAAAACCTACCGTTAGTGATTTTGTTCAAATTGAGAGTTGTGTGGTCACCTATGTCAATTTGACTAGAGACCAACTACCAGATGTAATCCCAGATTACATCGATGTTAACAAAACACTTGATGAGATTTTAGCTTCTCTGCCCAATAGAACTGGTCCAAGTCTTCCTTTAGATGTTTTTAATGCCACTTATCTTAATCTCACTGGTGAAATTGCAGATTTAGAGCAGCGTTCAGAGTCTCTCCGTAATACTACAGAGGAGCTCCAAAGTCTTATATATAATATCAACAACACACTAGTTGACCTTGAGTGGCTCAACCGAGTTGAGACATATATCAAGTGGCCGTGGTGGGTTTGGTTGATTATTTTCATTGTTCTCATCTTTGTTGTGTCATTACTAGTGTTCTGCTGCATTTCCACGGGTTGTTGTGGATGCTGCGGCTGCTGCTGTGCTTGTTTTTCAGGTTGTTGTAGGGGTCCTAGACTTCAACCTTACGAAGTTTTTGAAAAGGTCCACGTGCAGTGA

Protein

MKSLNYFWLFLPVLSTLSLPQDVTRCQSTINFRRFFSKFNVQAPAVVVLGGYLPSMNSSSWYCGTGLETASGVHGIFLSYIDAGQGFEIGISQEPFDPSGYQLYLHKATNGNHNAIARLRICQFPNNKTLGPTVNDVTTGRNCLFNKAIPAYMQDGKNIVVGITWDNDRVTVFADKIYHFYLKNDWSRVATRCYNKRSCAMQYVYTPTYYMLNVTSAGEDGIYYEPCTANCSGYAVNVFATDSNGHIPEGFSFNNWFLLSNDSTLLHGKVVSNQPLLVNCLLAIPKIYGLGQFFSFNQTMDGVCNGAAAQRAPEALRFNINDTSVILAEGSIVLHTALGTNLSFVCSNSSDPHLATFTIPLGATQVPYYCFLKVDTYNSTVYKFLAVLPPTVREIVITKYGDVYVNGFGYLHLGLLDAVTINFTGHGTDDDVSGFWTIASTNFVDALIEVQGTAIQRILYCDDPVSQLKCSQVAFDLDDGFYPISSRNLLSHEQPISFVTLPSFNDHSFVNITVSASFGGHSGANLIASDTTINGFSSFCVDTRQFTISLFYNVTNSYGYVSKSQDSNCPFTLQSVNDYLSFSKFCVSTSLLASACTIDLFGYPEFGSGVKFTSLYFQFTKGELITGTPKPLEGVTDVSFMTLDVCTKYTIYGFKGEGIITLTNSSFLAGVYYTSDSGQLLAFKNVTSGAVYSVTPCSFSEQAAYVDDDIVGVISSLSSSTFNSTRELPGFFYHSNDGSNCTEPVLVYSNIGVCKSGSIGYVPSQSGQVKIAPTVTGNISIPTNFSMSIRTEYLQLYNTPVSVDCATYVCNGNSRCKQLLTQYTAACKTIESALQLSARLESVEVNSMLTISEEALQLATISSFNGDGYNFTNVLGVSVYDPASGRVVQKRSFIEDLLFNKVVTNGLGTVDEDYKRCSNGRSVADLVCAQYYSGVMVLPGVVDAEKLHMYSASLIGGMVLGGFTSAAALPFSYAVQARLNYLALQTDVLQRNQQLLAESFNSAIGNITSAFESVKEAISQTSKGLNTVAHALTKVQEVVNSQGAALTQLTVQLQHNFQAISSSIDDIYSRLDILSADVQVDRLITGRLSALNAFVAQTLTKYTEVQASRKLAQQKVNECVKSQSQRYGFCGGDGEHIFSLVQAAPQGLLFLHTVLVPGDFIDVIAIAGLCVNDEIALTLREPGLVLFTHELQNHTATEYFVSSRRMFEPRKPTVSDFVQIESCVVTYVNLTRDQLPDVIPDYIDVNKTLDEILASLPNRTGPSLPLDVFNATYLNLTGEIADLEQRSESLRNTTEELQSLIYNINNTLVDLEWLNRVETYIKWPWWVWLIIFIVLIFVVSLLVFCCISTGCCGCCGCCCACFSGCCRGPRLQPYEVFEKVHVQ

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Uniprot

A0A0K1LUM5   A0A238TMH6   A0A0K1LUK6   A0A239QNQ6   A0A221HZH0   A0A238TPF5   + More
A0A239QPT4   A0A239QNW7   A0A0M5JV19   A0A0F6TKU6   A0A125S8N7   A0A238TMZ1   A0A238TN08   A0A238TM74   A0A1B3Q5X6   A0A0A8LEG6   A0A0A8LF79   A0A0U3HSZ7   A0A075EC92   A0A075ECF0   A0A5A4M655   A0A238TMM6   A0A239QPZ3   A0A0U3SHN8   A0A5J6KLN8   A0A0S3MR13   A0A238TMM2   A0A076MKN4   A0A0K2D774   A0A1L3KJ30   A0A238TN36   A0A2K8IYF0   W8Q548   A0A238TQ27   A0A5A4LZS2   A0A2R4H2S5   A0A0H4J507   A0A0H4A7A3   A0A2R4FC33   A0A3Q8TMG0   A0A0S3MSW7   A0A0K2D7V3   A0A223DMZ5   A0A0U3SAZ0   A0A0K2D763   A0A075EAW2   A0A5A4LZS9   A0A0M4HL64   A0A0E3XJT7   A0A2Z4QIT6   A0A1U9WZ00   A0A0K2D7U0   A0A219PSP4   A0A5A4M1Z1   A0A089WZ38   A0A0U3RFR3   A0A5A4MDF7   A0A142II42   A0A2U8QMX5   A0A223DMY9   A0A0S3MR49   K4P7K4   A0A125R587   A0A0K2D7L3   A0A5J6KL68   A0A0H4AE15   A0A2R4H2J3   A0A0K2D7G4   A0A0H4LTY7   A0A089X4N9   A0A223DMZ6   A0A2H5AC28   A0A3G2C516   S5TGV8   A0A5A4M1X5   A0A1C7A0X8   A0A0K2D7L7   A0A0M4HL41   A0A142II41   A0A2H5AC35   A0A0M4I404   A0A109QKG8   A0A5A4M032   A0A2U8QMK9   A0A5A4M1V6   A0A068BEA0   A0A5A4LZY2   A0A0H4M4S9   A0A2R4H2L0   A0A0H4AE20   A0A0H4LTX6   A0A0U3R9R6   A0A5A4M3B5   A0A0H4LQ15   I7FS14   A0A1B4XVK3   A0A142II40   J7LKT6   A0A0H4LS19   A0A0H4AFC2  

Pubmed

29981699    26714453    26044419    27056240    25279722    26477934    + More
25217400    29540176    27880757    24750580    28755162    25999551    29770590    26841768    29243063    25673903    27224981   

EMBL

KR011122    LT898415    LT898414    LT898411    LT898412    LT898425    + More
LT898427    LT898426    LT898438    LT898444    LT898417    AKU46227.1    SNQ27893.1    SNQ27911.1    SNQ27917.1    SNQ27955.1    SNQ27973.1    SNQ27985.1    SNQ28010.1    SNQ28022.1    SNQ28076.1    SNQ28082.1    LT898440    SNQ28032.1    KR011121    AKU46226.1    LT898413    LT900502    SNQ27899.1    SNT95671.1    KY381581    LT898447    ASM46788.1    SNQ28088.1    LT898408    SNQ27887.1    LT900500    SNT95665.1    LT900501    SNT95677.1    KT206204    LT898418    LT898433    LT898441    ALD19740.1    SNQ27931.1    SNQ27997.1    SNQ28051.1    KR011756    LT898416    LT898409    LT898410    LT898430    LT898446    LT900498    AKE47378.1    SNQ27923.1    SNQ27941.1    SNQ27961.1    SNQ27991.1    SNQ28064.1    SNT95653.1    KT895907    AME16503.1    LT898420    SNQ27949.1    LT898431    SNQ27978.1    LT898421    SNQ27905.1    KU985229    AOG30831.1    LM645057    CDW77205.1    LM645058    CDW77213.1    KU297956    ALU34112.1    KJ645635    KJ645702    KJ645704    LC063846    AID56667.1    AID57069.1    AID57081.1    BAT33329.1    KJ645655    AID56787.1    MG198633    AXS76457.1    LT898423    SNQ27930.1    LT898439    LT900499    SNQ28038.1    SNT95659.1    KT388412    ALV66164.1    MK648472    QEV88420.1    LC063845    BAT33323.1    LT898436    LT898435    SNQ28016.1    SNQ28045.1    KJ645695    KJ645696    KM196109    KM392232    KR265759    KR265760    KM975738    KM975739    KM975740    KY619745    AID57027.1    AID57033.1    AIJ19447.1    AIN52136.1    AKJ21701.1    AKJ21707.1    AKP80610.1    AKP80617.1    AKP80624.1    ATJ03600.1    KP870128    ALA15721.1    KX883635    APG77336.1    LT898445    SNQ28070.1    KY619748    ATJ03603.1    KJ399978    AHL38184.1    LT898432    LT898443    SNQ27967.1    SNQ28057.1    MG198641    AXS76465.1    MH006958    AVU05417.1    KR265761    KP688354    AKJ21713.1    AKO63237.1    KR296677    AKN45979.1    MF038017    AVT28076.1    MH061336    AZL47192.1    LC063844    BAT33317.1    KP870140    ALA15733.1    KY775053    ASS83140.1    KT388414    ALV66166.1    KP870118    ALA15711.1    KJ645649    AID56751.1    MG198651    MG198664    AXS76475.1    AXS76488.1    KT206206    ALD19742.1    KR003452    AKC34872.1    MF974246    AWY10515.1    KY111278    AQY61719.1    KP870125    ALA15718.1    KT591944    AMD11118.1    MG198665    AXS76489.1    KM403155    KM403156    KM403157    AIR95864.1    AIR95870.1    AIR95871.1    KT388409    ALV66161.1    MG198638    AXS76462.1    KU133258    AMR59387.1    MH052680    AWM11503.1    KY775054    ASS83141.1    LC063847    BAT33335.1    JX501318    AFV59240.1    KT860508    AMD11124.1    KP870131    ALA15724.1    MK648471    QEV88419.1    KR296673    KP870138    AKN45975.1    ALA15731.1    MH006960    AVU05431.1    KP870132    ALA15725.1    KP698769    AKP16768.1    KM403158    AIR95872.1    KY775052    ASS83139.1    KY828991    AUG68851.1    MG706122    AYM47397.1    KF453513    AGS46488.1    MG198655    AXS76479.1    KM975741    AKP80631.1    KP870136    ALA15729.1    KP399614    ALD19703.1    KU133257    AMR59386.1    KY828996    AUG68856.1    KT206205    ALD19741.1    KT428878    AMB20700.1    MG198634    AXS76458.1    MH052681    AWM11456.1    MG198645    AXS76469.1    KJ642641    AIC83850.1    MG198636    AXS76460.1    KP698763    AKP16762.2    MH006962    AVU05445.1    KR296678    AKN45980.1    KP698759    AKP16758.1    KT388420    ALV66172.1    MG198659    AXS76483.1    KP698760    AKP16759.1    JX070672    AFP25201.1    LC113937    BAV38832.1    KU133256    AMR59385.1    JX242462    AFR11480.1    KP698761    AKP16760.2    KR296680    AKN45982.1   

Proteomes

UP000270191    UP000270545    UP000273819    UP000274834    UP000275549    UP000275656    + More
UP000275831    UP000276052    UP000278039    UP000278495    UP000267306    UP000270497    UP000274353    UP000271135    UP000277927    UP000271448    UP000281935    UP000271078    UP000274992    UP000278554    UP000100204    UP000267271    UP000276298    UP000277020    UP000279022    UP000280517    UP000281047    UP000269867    UP000267609    UP000268721    UP000138211    UP000155002    UP000132054    UP000122852    UP000136506    UP000147669    UP000153418    UP000151262    UP000277354    UP000269355    UP000274124    UP000127975    UP000278990    UP000280596    UP000096695    UP000120524    UP000123161    UP000154382    UP000154704    UP000281631    UP000107011    UP000272790    UP000276964    UP000096896    UP000107726    UP000119410    UP000161506    UP000166734    UP000109281    UP000107825    UP000173775   

Pfam

PF01600   Corona_S1
PF01601   Corona_S2

Interpro

IPR002552   Corona_S2
IPR042552   ALPHA_CORONA_SPIKE
IPR002551   Corona_S1

ProteinModelPortal

A0A0K1LUM5   A0A238TMH6   A0A0K1LUK6   A0A239QNQ6   A0A221HZH0   A0A238TPF5   + More
A0A239QPT4   A0A239QNW7   A0A0M5JV19   A0A0F6TKU6   A0A125S8N7   A0A238TMZ1   A0A238TN08   A0A238TM74   A0A1B3Q5X6   A0A0A8LEG6   A0A0A8LF79   A0A0U3HSZ7   A0A075EC92   A0A075ECF0   A0A5A4M655   A0A238TMM6   A0A239QPZ3   A0A0U3SHN8   A0A5J6KLN8   A0A0S3MR13   A0A238TMM2   A0A076MKN4   A0A0K2D774   A0A1L3KJ30   A0A238TN36   A0A2K8IYF0   W8Q548   A0A238TQ27   A0A5A4LZS2   A0A2R4H2S5   A0A0H4J507   A0A0H4A7A3   A0A2R4FC33   A0A3Q8TMG0   A0A0S3MSW7   A0A0K2D7V3   A0A223DMZ5   A0A0U3SAZ0   A0A0K2D763   A0A075EAW2   A0A5A4LZS9   A0A0M4HL64   A0A0E3XJT7   A0A2Z4QIT6   A0A1U9WZ00   A0A0K2D7U0   A0A219PSP4   A0A5A4M1Z1   A0A089WZ38   A0A0U3RFR3   A0A5A4MDF7   A0A142II42   A0A2U8QMX5   A0A223DMY9   A0A0S3MR49   K4P7K4   A0A125R587   A0A0K2D7L3   A0A5J6KL68   A0A0H4AE15   A0A2R4H2J3   A0A0K2D7G4   A0A0H4LTY7   A0A089X4N9   A0A223DMZ6   A0A2H5AC28   A0A3G2C516   S5TGV8   A0A5A4M1X5   A0A1C7A0X8   A0A0K2D7L7   A0A0M4HL41   A0A142II41   A0A2H5AC35   A0A0M4I404   A0A109QKG8   A0A5A4M032   A0A2U8QMK9   A0A5A4M1V6   A0A068BEA0   A0A5A4LZY2   A0A0H4M4S9   A0A2R4H2L0   A0A0H4AE20   A0A0H4LTX6   A0A0U3R9R6   A0A5A4M3B5   A0A0H4LQ15   I7FS14   A0A1B4XVK3   A0A142II40   J7LKT6   A0A0H4LS19   A0A0H4AFC2  

PDB

6U7K     E-value=0,     Score=6556