Strain

BELGIUM_PIG_1978_LT906581 (Region: Belgium;  Strain: Porcine epidemic diarrhea virus isolate CV777 genome assembly, complete genome: monopartite.;  Date: 1978)

Gene

spike protein

Description

Annotated in NCBI,  spike protein

Location

LT906581(+):20574-24725

GenBank Accession

SNW64190.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGAGGTCTTTAATTTACTTCTGGTTGCTCTTACCAGTACTTCCAACACTCAGCCTACCACAAGATGTCACTAGGTGCCAGTCTACTACTAACTTTAGGCGGTTCTTTTCAAAATTTAATGTTCAGGCACCTGCCGTCGTCGTTTTGGGTGGTTACCTACCTAGTATGAACTCTTCTAGCTGGTACTGTGGCACAGGCATTGAAACTGCTAGTGGCGTTCATGGTATTTTTCTCAGCTACATCGATTCTGGTCAGGGCTTTGAGATTGGCATTTCGCAAGAGCCGTTTGATCCTAGTGGTTACCAGCTTTATTTACATAAGGCCACTAATGGTAACACTAATGCTATTGCACGACTGCGCATTTGCCAGTTTCCCGATAATAAAACATTGGGCCCTACTGTTAATGATGTTACAACAGGTCGTAACTGCCTATTCAACAAAGCCATTCCAGCTTATATGCGTGATGGAAAAGATATTGTTGTCGGCATAACATGGGATAATGATCGTGTCACTGTTTTTGCTGACAAGATCTATCATTTTTATCTTAAAAATGATTGGTCCCGCGTTGCGACAAGGTGTTACAATCGCAGAAGTTGTGCTATGCAATATGTTTATACACCTACCTACTACATGCTTAATGTTACTAGTGCAGGTGAGGATGGCATTTATTATGAACCCTGTACAGCTAATTGCACTGGTTACGCTGCCAATGTATTTGCCACTGATTCCAATGGCCATATACCAGAAGGTTTTAGTTTTAATAATTGGTTTCTTTTATCCAATGACTCCACTTTGTTGCATGGTAAAGTGGTTTCCAACCAACCCTTGTTGGTCAATTGTCTTTTGGCCATTCCTAAGATTTATGGACTAGGCCAATTTTTCTCATTCAATCACACGATGGATGGCGTTTGTAATGGAGCTGCTGTGGATCGTGCCCCAGAGGCTCTGAGGTTTAATATTAATGACACCTCCGTCATTCTTGCTGAAGGCTCAATTGTACTTCATACTGCTTTAGGAACAAATCTTTCTTTTGTTTGCAGTAATTCCTCAGATCCTCATTTAGCCATCTTTGCCATACCTCTGGGTGCTACTGAAGTACCCTACTATTGCTTTCTTAAAGTGGATACTTACAACTCCACTGTTTATAAATTCTTGGCTGTTTTACCTCCTACTGTCAGGGAAATTGTCATCACCAAGTATGGTGATGTTTATGTCAATGGGTTTGGCTATTTGCATCTCGGTTTGTTGGATGCTGTCACAATTAATTTCACTGGTCATGGCACTGACGATGACGTTTCAGGTTTCTGGACCATAGCATCGACTAATTTTGTTGATGCACTCATCGAGGTTCAAGGAACTTCCATTCAGCGTATTCTTTATTGTGATGATCCTGTTAGCCAACTCAAGTGTTCTCAGGTTGCTTTTGACCTTGACGATGGTTTTTACCCCATCTCTTCTAGAAACCTTCTGAGTCACGAACAGCCAATTTCTTTTGTTACTTTGCCATCATTTAATGATCATTCTTTTGTTAATATTACTGTCTCTGCGGCTTTTGGTGGTCTTAGTAGTGCCAATCTCGTTGCATCTGACACTACTATCAATGGGTTTAGTTCTTTCTGTGTTGACACTAGACAATTTACCATTACACTGTTTTATAATGTTACAAACAGTTATGGTTATGTGTCTAAATCACAGGATAGTAATTGTCCTTTCACCTTGCAATCTGTTAATGATTACCTGTCTTTTAGCAAATTTTGTGTTTCAACCAGCCTTTTGGCTGGTGCTTGTACCATAGATCTTTTTGGTTACCCTGCGTTCGGTAGTGGTGTTAAGTTGACGTCCCTTTATTTTCAATTCACAAAAGGTGAGTTGATTACTGGCACGCCTAAACCACTTGAAGGTATCACAGACGTTTCTTTTATGACTCTGGATGTGTGTACCAAGTATACTATCTATGGCTTTAAAGGTGAGGGTATTATTACCCTTACAAATTCTAGCATTTTGGCAGGTGTTTATTATACATCTGATTCTGGACAGTTGTTAGCCTTTAAGAATGTCACTAGTGGTGCTGTTTATTCTGTCACGCCATGTTCTTTTTCAGAGCAGGCTGCATATGTTAATGATGATATAGTGGGTGTTATTTCTAGTTTGTCTAACTCCACTTTTAACAATACTAGGGAGTTGCCTGGTTTCTTCTACCATTCTAATGACGGCTCCAATTGTACAGAGCCTGTGTTGGTGTATAGTAACATAGGTGTTTGTAAATCTGGCAGTATTGGCTATGTTCCATCTCAGTATGGCCAAGTCAAGATTGCACCCACGGTTACTGGGAATATTAGTATTCCCACCAACTTTAGTATGAGTATTAGAACAGAATATTTACAGCTTTACAACACGCCTGTTAGTGTTGATTGTGCTACATATGTTTGTAATGGTAACTCTCGTTGTAAACAATTACTCACCCAGTACACTGCAGCATGTAAGACCATAGAGTCAGCATTACAACTCAGCGCTAGGCTTGAGTCTGTTGAAGTTAACTCTATGCTTACCATTTCTGAAGAGGCTTTACAGTTAGCTACCATCAGTTCGTTTAATGGTGATGGATATAACTTTACTAATGTGCTGGGTGCTTCCGTGTACGATCCTGCAAGTGGCAGGGTGGTACAAAAAAGGTCTGTTATTGAAGACTTGCTTTTTAATAAAGTGGTTACTAATGGCCTTGGTACTGTTGATGAAGACTATAAGCGCTGTTCTAATGGTCGCTCTGTGGCTGATCTAGTCTGTGCGCAGTATTACTCTGGTGTCATGGTACTACCTGGCGTTGTTGACGCTGAGAAGCTTCACATGTACAGTGCGTCTCTCATAGGTGGTATGGCGCTAGGAGGTATAACTGCTGCAGCGGCATTGCCTTTTAGCTATGCTGTTCAAGCGAGACTCAATTATCTTGCTTTACAGACGGATGTTCTACAGCGGAACCAGCAATTGCTTGCTGAGTCTTTTAACTCTGCTATTGGTAATATAACTTCAGCCTTTGAGAGTGTTAAAGAGGCTATTAGTCAAACTTCCAAGGGTTTGAACACTGTGGCTCATGCGCTTACTAAGGTTCAAGAGGTTGTTAATTCGCAGGGTTCAGCTTTGAACCAACTTACCGTACAGCTGCAACACAACTTCCAAGCCATTTTTAGTTCTATTGATGACATTTATTCCCGACTGGACATTCTTTCAGCCGATGTTCAGGTTGATCGTATCATCACCGGCAGATTATCAGCACTTAATGCTTTTGTTGCCCAAACCCTCACTAAGTATACTGAGGTTCAGGCTAGCAGGAAGCTAGCACAGCAAAAGGTTAATGAGTGCGTCAAATCGCAATCTCAGCGTTACGGTTTTTGTGGTGGTGATGGCGAGCACATTTTCTCTCTGGTACAGGCCGCACCTCAGGGCCTGCTGTTCTTACATACAGTACTTGTACCGGGTGATTTTGTAAATGTTCTTGCCATCGCTGGCTTATGCGTTAATGGTGAAATTGCCTTGACTCTACGTGAGCCTGGCTTAGTCTTGTTTACGCATGAACTTCAAACTTATACTGCGACGGAATATTTTGTTTCATCGCGACGTATGTTTGAACCTAGAAAACCTACCGTTAGTGATTTTGTTCAAATTGAGAGTTGTGTGGTCACCTATGTCAATCTGACTAGCGACCAGCTACCAGATGTAATCCCAGATTACATCGATGTTAACAAAACACTTGATGAGATTTTAGCTTCTCTGCCCAATAGAACTGGTCCAAGTCTTCCCCTAGATGTTTTTAATGCCACTTATCTTAATCTTACTGGTGAAATTGCAGATCTAGAGCAGCGTTCAGAGTCTCTCCGTAATACTACAGAAGAGCTCCGAAGTCTCATTAACAACATCAACAACACACTTGTTGACCTTGAGTGGCTCAACCGAGTTGAGACATACATCAAGTGGCCGTGGTGGGTTTGGTTGATCATTGTTATTGTTCTCATCTTTGTTGTGTCATTACTAGTGTTCTGCTGCATTTCCACGGGTTGTTGTGGATGCTGCGGTTGCTGCGGTGCTTGTTTTTCAGGTTGTTGTAGGGGTCCTAGACTTCAACCTTACGAAGCTTTTGAAAAGGTCCACGTGCAGTGA

Protein

MRSLIYFWLLLPVLPTLSLPQDVTRCQSTTNFRRFFSKFNVQAPAVVVLGGYLPSMNSSSWYCGTGIETASGVHGIFLSYIDSGQGFEIGISQEPFDPSGYQLYLHKATNGNTNAIARLRICQFPDNKTLGPTVNDVTTGRNCLFNKAIPAYMRDGKDIVVGITWDNDRVTVFADKIYHFYLKNDWSRVATRCYNRRSCAMQYVYTPTYYMLNVTSAGEDGIYYEPCTANCTGYAANVFATDSNGHIPEGFSFNNWFLLSNDSTLLHGKVVSNQPLLVNCLLAIPKIYGLGQFFSFNHTMDGVCNGAAVDRAPEALRFNINDTSVILAEGSIVLHTALGTNLSFVCSNSSDPHLAIFAIPLGATEVPYYCFLKVDTYNSTVYKFLAVLPPTVREIVITKYGDVYVNGFGYLHLGLLDAVTINFTGHGTDDDVSGFWTIASTNFVDALIEVQGTSIQRILYCDDPVSQLKCSQVAFDLDDGFYPISSRNLLSHEQPISFVTLPSFNDHSFVNITVSAAFGGLSSANLVASDTTINGFSSFCVDTRQFTITLFYNVTNSYGYVSKSQDSNCPFTLQSVNDYLSFSKFCVSTSLLAGACTIDLFGYPAFGSGVKLTSLYFQFTKGELITGTPKPLEGITDVSFMTLDVCTKYTIYGFKGEGIITLTNSSILAGVYYTSDSGQLLAFKNVTSGAVYSVTPCSFSEQAAYVNDDIVGVISSLSNSTFNNTRELPGFFYHSNDGSNCTEPVLVYSNIGVCKSGSIGYVPSQYGQVKIAPTVTGNISIPTNFSMSIRTEYLQLYNTPVSVDCATYVCNGNSRCKQLLTQYTAACKTIESALQLSARLESVEVNSMLTISEEALQLATISSFNGDGYNFTNVLGASVYDPASGRVVQKRSVIEDLLFNKVVTNGLGTVDEDYKRCSNGRSVADLVCAQYYSGVMVLPGVVDAEKLHMYSASLIGGMALGGITAAAALPFSYAVQARLNYLALQTDVLQRNQQLLAESFNSAIGNITSAFESVKEAISQTSKGLNTVAHALTKVQEVVNSQGSALNQLTVQLQHNFQAIFSSIDDIYSRLDILSADVQVDRIITGRLSALNAFVAQTLTKYTEVQASRKLAQQKVNECVKSQSQRYGFCGGDGEHIFSLVQAAPQGLLFLHTVLVPGDFVNVLAIAGLCVNGEIALTLREPGLVLFTHELQTYTATEYFVSSRRMFEPRKPTVSDFVQIESCVVTYVNLTSDQLPDVIPDYIDVNKTLDEILASLPNRTGPSLPLDVFNATYLNLTGEIADLEQRSESLRNTTEELRSLINNINNTLVDLEWLNRVETYIKWPWWVWLIIVIVLIFVVSLLVFCCISTGCCGCCGCCGACFSGCCRGPRLQPYEAFEKVHVQ

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

3D-structure   Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

A0A2N8Z212   A0A2N8Z1W1   Q91AV1   Q0GJJ0   Q84712   A0A2N8Z1U7   + More
A0A2N8Z1T8   A0A2N8Z1U0   A0A2N8YGD1   A2IB75   A0A0U1YFZ0   I1TLC3   A0A1B2RVC6   A0A0B5WYJ6   A0A0F7R7M9   F2VPY2   A0A286L9N9   A0A286L9M7   A0A288Y8U4   A0A286L9L5   A0A286L9K9   F5HNC9   A0A0F7L1H5   F5HND0   W6E935   A0A286L9N3   A0A5A4MDF7   F5HND1   A0A0U2SJS6   A0A0U3HSZ7   A0A239QPZ3   G5D078   A0A3Q8TMG0   A0A286L9M1   A0A0H4A7A7   A0A238TMM2   A0A2R4FC33   A0A068BEA0   A0A075ECF0   A0A075EAW2   A0A075EC92   A0A0H4LTY7   A0A109QKG8   I2EBM8   A0A0K2D763   A0A076MKN4   A0A221HZH0   A0A5A4M655   A0A0S3MR13   A0A0H4LTX6   A0A238TN36   I7FS14   A0A142II42   A0A0M4HL41   A0A089WZ38   A0A0K2D774   A0A239QNQ6   A0A0H4J507   A0A0A8LEG6   W8Q548   A0A0A8LF79   Q692M1   A0A0S3MR49   A0A2K8IYF0   A0A0H4A7A3   A0A223DMZ5   A0A3G2C516   A0A0K1LUM5   A0A0H4LQ15   A0A0M4I404   A0A2R4H2S5   A0A089X4N9   S5TGV8   A0A238TMH6   A0A0K1LUK6   A0A5A4M1T6   A0A0M5JV19   A0A3S8TLM6   A0A2U8QMX5   A0A125R587   F5HND5   A0A0S3MSW7   K4P7K4   J7LK74   A0A0U3SAZ0   A0A0K2D7U0   A0A5A4M032   S4V9P2   A0A238TPF5   A0A1P8A7E4   F5HND2   A0A239QPT4   A0A1L3KJ30   A0A219PSP4   A0A5A4LZY2   K4G1P9   A0A1B3Q5X6   J7LKT6   A0A0H4M4S9   A0A0H4LS19  

Pubmed

8291230    9782358    11724265    17072758    8397280    8176382    + More
22532530    28854955    21559974    26112783    27056240    21980030    25279722    26477934    25217400    29540176    24750580    28755162    29981699    26714453    25673903    29770590    26841768    27880757   

EMBL

LT906581    SNW64190.1    LT906620    SNX30664.1    AF353511    DQ862099    + More
ABI30278.1    Z25483    CAA80971.1    LT906582    SNW64184.1    LT905450    SNU46336.1    LT905451    SNU46342.1    LT897799    SNC44845.1    EF185992    ABM64776.1    KJ857455    AJD09578.1    JQ023161    AFE85962.1    KU664503    AOC38021.1    KM887144    AJH76957.1    LC053455    BAR73378.1    GU937797    ADZ76334.1    KX839251    AST13245.1    KY486713    KX839249    AST13130.1    AST13233.1    KX839247    AST13221.1    KX839246    AST13215.1    KY486714    AST13136.1    AB548618    BAK32938.1    KR610991    KR610992    AKH45332.1    AB548619    BAK32939.1    KF898124    AHJ11040.1    KX839250    AST13239.1    MG198638    AXS76462.1    AB548620    BAK32940.1    KR061458    ALR84908.1    KU297956    ALU34112.1    LT898439    LT900499    SNQ28038.1    SNT95659.1    JN547228    AEQ55004.1    MH061336    AZL47192.1    KX839248    AST13227.1    KR296682    AKN45984.1    LT898436    LT898435    SNQ28016.1    SNQ28045.1    MF038017    AVT28076.1    KJ642641    AIC83850.1    KJ645655    AID56787.1    KJ645649    AID56751.1    KJ645635    KJ645702    KJ645704    LC063846    AID56667.1    AID57069.1    AID57081.1    BAT33329.1    KP698769    AKP16768.1    KT428878    AMB20700.1    JQ638923    AFJ97038.1    KP870118    ALA15711.1    KJ645695    KJ645696    KM196109    KM392232    KR265759    KR265760    KM975738    KM975739    KM975740    KY619745    AID57027.1    AID57033.1    AIJ19447.1    AIN52136.1    AKJ21701.1    AKJ21707.1    AKP80610.1    AKP80617.1    AKP80624.1    ATJ03600.1    KY381581    LT898447    ASM46788.1    SNQ28088.1    MG198633    AXS76457.1    LC063845    BAT33323.1    KP698759    AKP16758.1    LT898445    SNQ28070.1    JX070672    AFP25201.1    KU133258    AMR59387.1    KP399614    ALD19703.1    KM403155    KM403156    KM403157    AIR95864.1    AIR95870.1    AIR95871.1    KP870128    ALA15721.1    LT898413    LT900502    SNQ27899.1    SNT95671.1    KR265761    KP688354    AKJ21713.1    AKO63237.1    LM645057    CDW77205.1    KJ399978    AHL38184.1    LM645058    CDW77213.1    AY653204    AAT75298.1    LC063847    BAT33335.1    KY619748    ATJ03603.1    KR296677    AKN45979.1    KY775053    ASS83140.1    MG706122    AYM47397.1    KR011122    LT898415    LT898414    LT898411    LT898412    LT898425    LT898427    LT898426    LT898438    LT898444    LT898417    AKU46227.1    SNQ27893.1    SNQ27911.1    SNQ27917.1    SNQ27955.1    SNQ27973.1    SNQ27985.1    SNQ28010.1    SNQ28022.1    SNQ28076.1    SNQ28082.1    KP698760    AKP16759.1    KT206205    ALD19741.1    MH006958    AVU05417.1    KM403158    AIR95872.1    KF453513    AGS46488.1    LT898440    SNQ28032.1    KR011121    AKU46226.1    MG198635    AXS76459.1    KT206204    LT898418    LT898433    LT898441    ALD19740.1    SNQ27931.1    SNQ27997.1    SNQ28051.1    MH593141    AZL47242.1    MH052680    AWM11503.1    KT860508    AMD11124.1    AB548624    BAK32944.1    LC063844    BAT33317.1    JX501318    AFV59240.1    JX242464    KP399632    AFR11482.1    ALD19721.1    KT388414    ALV66166.1    KP870125    ALA15718.1    MG198634    AXS76458.1    KC787543    AGO59793.1    LT898408    SNQ27887.1    KU975401    AMR60838.1    KY619780    AB548621    ATJ03635.1    BAK32941.1    LT900500    SNT95665.1    KX883635    APG77336.1    KT591944    AMD11118.1    MG198636    AXS76460.1    JQ979287    AFM55051.1    KU985229    AOG30831.1    JX242462    AFR11480.1    KP698763    AKP16762.2    KP698761    AKP16760.2   

Proteomes

UP000269222    UP000276154    UP000008159    UP000274194    UP000278337    UP000281783    + More
UP000267509    UP000170862    UP000122353    UP000110433    UP000096210    UP000101744    UP000156820    UP000171486    UP000124407    UP000132054    UP000269355    UP000274124    UP000095955    UP000278990    UP000280596    UP000151262    UP000161506    UP000122852    UP000136506    UP000147669    UP000153418    UP000096695    UP000120524    UP000123161    UP000154382    UP000154704    UP000271135    UP000127975    UP000281631    UP000109281    UP000270497    UP000274353    UP000096896    UP000107726    UP000138211    UP000107011    UP000155002    UP000107825    UP000270191    UP000270545    UP000273819    UP000274834    UP000275549    UP000275656    UP000275831    UP000276052    UP000278039    UP000278495    UP000267306    UP000271078    UP000274992    UP000278554    UP000173775    UP000119410    UP000277927    UP000271448   

PRIDE

Q91AV1   

Pfam

PF01601   Corona_S2
PF01600   Corona_S1

Interpro

IPR002552   Corona_S2
IPR042552   ALPHA_CORONA_SPIKE
IPR002551   Corona_S1

ProteinModelPortal

A0A2N8Z212   A0A2N8Z1W1   Q91AV1   Q0GJJ0   Q84712   A0A2N8Z1U7   + More
A0A2N8Z1T8   A0A2N8Z1U0   A0A2N8YGD1   A2IB75   A0A0U1YFZ0   I1TLC3   A0A1B2RVC6   A0A0B5WYJ6   A0A0F7R7M9   F2VPY2   A0A286L9N9   A0A286L9M7   A0A288Y8U4   A0A286L9L5   A0A286L9K9   F5HNC9   A0A0F7L1H5   F5HND0   W6E935   A0A286L9N3   A0A5A4MDF7   F5HND1   A0A0U2SJS6   A0A0U3HSZ7   A0A239QPZ3   G5D078   A0A3Q8TMG0   A0A286L9M1   A0A0H4A7A7   A0A238TMM2   A0A2R4FC33   A0A068BEA0   A0A075ECF0   A0A075EAW2   A0A075EC92   A0A0H4LTY7   A0A109QKG8   I2EBM8   A0A0K2D763   A0A076MKN4   A0A221HZH0   A0A5A4M655   A0A0S3MR13   A0A0H4LTX6   A0A238TN36   I7FS14   A0A142II42   A0A0M4HL41   A0A089WZ38   A0A0K2D774   A0A239QNQ6   A0A0H4J507   A0A0A8LEG6   W8Q548   A0A0A8LF79   Q692M1   A0A0S3MR49   A0A2K8IYF0   A0A0H4A7A3   A0A223DMZ5   A0A3G2C516   A0A0K1LUM5   A0A0H4LQ15   A0A0M4I404   A0A2R4H2S5   A0A089X4N9   S5TGV8   A0A238TMH6   A0A0K1LUK6   A0A5A4M1T6   A0A0M5JV19   A0A3S8TLM6   A0A2U8QMX5   A0A125R587   F5HND5   A0A0S3MSW7   K4P7K4   J7LK74   A0A0U3SAZ0   A0A0K2D7U0   A0A5A4M032   S4V9P2   A0A238TPF5   A0A1P8A7E4   F5HND2   A0A239QPT4   A0A1L3KJ30   A0A219PSP4   A0A5A4LZY2   K4G1P9   A0A1B3Q5X6   J7LKT6   A0A0H4M4S9   A0A0H4LS19  

PDB

6U7K     E-value=0,     Score=6556