Strain

SWITZERLAND_PIG_2003_LT905451 (Region: Switzerland;  Strain: Porcine epidemic diarrhea virus isolate CV777 genome assembly, complete genome: monopartite.;  Date: 2003)

Gene

spike protein

Description

Annotated in NCBI,  spike protein

Location

LT905451(+):20609-24739

GenBank Accession

SNU46342.1

Full name

Spike glycoprotein      

Alternative Name

E2
Peplomer protein

CDS

ATGAGGTCTTTAATTTACTTCTGGTTGCTCTTACCAGTACTTCCAACACTCAGCCTACCACAAGATGTCACTAGGTGCCAGTCTACTACTAACTTTAGGCGGTTCTTTTCAAAATTTAATGTTCAGGCACCTGCCGTCGTCGTTTTGGGTGGTTACCTACCTAGTATGAACTCTTCTAGCTGGTACTGTGGCACAGGCATTGAAACTGCTAGTGGCGTTCATGGTATTTTTCTCAGCTACATCGATTCTAGTCAGGGCTTTGAGATTGGCATTTCGCAAGAGCCGTTTGATCCTAGTGGTTACCAGCTTTATTTACATAAGGCCACTAATGGTAACACTAATGCTATTGCACGACTGCGCATTTGCCAGTTTCCCGATAATAAAACATTGGGCCCTACTGTTAATGATGTTACAACAGGTCGTAACTGCCTATTCAACAAAGCCATTCCAGCTTATATGCGTGATGGAAAAGATATTGTTGTCGGCATAACATGGGATAATGATCGTGTCACTGTTTTTGCTGACAAGATCTATCATTTTTATCTTAAAAATGATTGGTCCCGCGTTGCGACAAGATGTTACAATCGCAGAAGTTGTGCTATGCAATATGTTTATACACCTACCTACTACATGCTTAATGTTACTAGTGCAGGTGAGGATGGCATTTATTATGAACCCTGTACAGCTAATTGCACTGGTTACGCTGCCAATGTATTTGCCACTGATTCCAATGGCCATATACCAGAAGGTTTTAGTTTTAATAATTGGTTTCTTTTATCCAATGACTCCACTTTGTTGCATGGTAAAGTGGTTTCCAACCAACCCTTGTTGGTCAATTGTCTTTTGGCCATTCCTAAGATTTATGGACTAGGCCAATTTTTCTCATTCAATCACACGATGGATGGCGTTTGTAATGGAGCTGCTGTGGATCGTGCCCCAGAGGCTCTGAGGTTTAATATTAATGACACCTCCGTCATTCTTGCTGAAGGCTCAATTGTACTTCATACTGCTTTAGGAACAAATCTTTCTTTTGTTTGCAGTAATTCCTCAGATCCTCATTTAGCCATCTTTGCCATACCTCTGGGTGCTACTGAAGTACCCTACTATTGCTTTCTTAAAGTGGATACTTACAACTCCACTGTTTATAAATTCTTGGCTGTTTTACCTCCTACTGTCAGGGAAATTGTCATCACCAAGTATGGTGATGTTTATGTCAATGGGTTTGGCTATTTGCATCTCGGTTTGTTGGATGCTGTCACAATTAATTTCACTGGTCATGGCACTGACGATGACGTTTCAGGTTTCTGGACCATAGCATCGACTAATTTTGTTGATGCACTCATCGAGGTTCAAGGAACTTCCATTCAGCGTATTCTTTATTGTGATGATCCTGTTAGCCAACTCAAGTGTTCTCAGGTTGCTTTTGACCTTGACGATGGTTTTTACCCCATCTCTTCTAGAAACCTTCTGAGTCACGAACAGCCAATTTCTTTTGTTACTTTGCCATTATTTAATGATCATTCTTTTGTTAATATTACTGTCTCTGCGGCTTTTGGTGGTCTTAGTAGTGCCAATCTCGTTGCATCTGACACTACTATCAATGGGTTTAGTTCTTTCTGTGTTGACACTAGACAATTTACCATTACACTGTTTTATAATGTTACAAACAGTTATGGTTATGTGTCTAAATCACAGGATAGTAATTGTCCTTTCACCTTGCAATCTGTTAATGATTACCTGTCTTTTAGCAAATTTTGTGTTTCAACCAGCCTTTTGGCTGGTGCTTGTACCATAGATCTTTTTGGTTACCCTGCGTTCGGTAGTGGTGTTAAGTTGACGTCCCTTTATTTTCAATTCACAAAAGGTGAGTTGATTACTGGCACGCCTAAACCACTTGAAGGTATCACAGACGTTTCTTTTATGACTCTGGATGTGTGTACCAAGTATACTATCTATGGCTTTAAAGGTGAGGGTATTATTACCCTTACAAATTCTAGCATTTTGGCAGGTGTTTATTATACATCTGATTCTGGACAGTTGTTAGCCTTTAAGAATGTCACTAGTGGTGCTGTTTATTCTGTCACGCCATGTTCTTTTTCAGAGCAGGCTGCATATGTTAATGATGATATAGTGGGTGTTATTTCTAGTTTGTCTAACTCCACTTTTAACAATACTAGGGAGTTGCCTGGTTTCTTCTACCATTCTAATGACGGCTCCAATTGTACAGAGCCTGTGTTGGTGTATAGTAACATAGGTGTTTGTAAATCTGGCAGTATTGGCTATGTTCCATCTCAGTATGGCCAAGTCAAGATTGCACCCACGGTTACTGGGAATATTAGTATTCCCACCAACTTTAGTATGAGTATTAGAACAGAATATTTACAGCTTTACAACACGCCTGTTAGTGTTGATTGTGCTACATATGTTTGTAATGGTAACTCTCGTTGTAAACAATTACTCACCCAGTACACTGCAGCATGTAAGACCATAGAGTCAGCATTACAACTCAGCGCTAGGCTTGAGTCTGTTGAAGTTAACTCTATGCTTACCATTTCTGAAGAGGCTTTACAGTTAGCTACCATCAGTTCGTTTAATGGTGATGGATATAACTTTACTAATGTGCTGGGTGCTTCCGTGTACGATCCTGCAAGTGGCAGGGTGGTACAAAAAAGGTCTGTTATTGAAGACTTGCTTTTTAATAAAGTGGTTACTAATGGCCTTGGTACTGTTGATGAAGACTATAAGCGCTGTTCTAATGGTCGCTCTGTGGCTGATCTAGTCTGTGCGCAGTATTACTCTGGTGTCATGGTACTACCTGGCGTTGTTGACGCTGAGAAGCTTCACATGTACAGTGCGTCTCTCATAGGTGGTATGGCGCTAGGAGGTATAACTGCTGCAGCGGCATTGCCTTTTAGCTATGCTGTTCAAGCGAGACTCAATTATCTTGCTTTACAGACGGATGTTCTACAGCGGAACCAGCAATTGCTTGCTGAGTCTTTTAACTCTGCTATTGGTAATATAACTTCAGCCTTTGAGAGTGTTAAAGAGGCTATTAGTCAAACTTCCAAGGGTTTGAACACTGTGGCTCATGCGCTTACTAAGGTTCAAGAGGTTGTTAATTCGCAGGGTTCAGCTTTGAACCAACTTACCGTACAGCTGCAACACAACTTCCAAGCCATTTCTAGTTCTATTGATGACATTTATTCCCGACTGGACATTCTTTCAGCCGATGTTCAGGTTGATCGTCTCATCACCGGCAGATTATCAGCACTTAATGCTTTTGTTGCCCAAACCCTCACTAAGTATACTGAGGTTCAGGCTAGCAGGAAGCTAGCACAGCAAAAGGTTAATGAGTGCGTCAAATCGCAATCTCAGCGTTACGGTTTTTGTGGTGGTGATGGCGAGCACATTTTCTCTCTGGTACAGGCCGCACCTCAGGGCCTGCTGTTCTTACATACAGTACTTGTACCGGGTGATTTTGTAAATGTTCTTGCCATCGCTGGCTTATGCGTTAATGGTGAAATTGCCTTGACTCTACGTGAGCCTGGCTTAGTCTTGTTTACGCATGAACTTCAAACTTATACTGCGACGGAATATTTTGTTTCATCGCGACGTATGTTTGAACCTAGAAAACCTACCGTTAGTGATTTTGTTCAAATTGAGAGTTGTGTGGTCACCTATGTCAATCTGACTAGCGACCAGCTACCAGATGTAATCCCAGATTACATCGATGTTAACAAAACACTTGATGAGATTTTAGCTTCTCTGCCCAATAGAACTGGTCCAAGTCTTCCCCTAGATGTTTTTAATGCCACTTATCTTAATCTTACTGGTGAAATTGCAGATCTAGAGCAGCGTTCAGAGTCTCTCCGTAATACTACAGAAGAGCTCCGAAGTCTCATTAACAACATCAACAACACACTTGTTGACCTTGAGTGGCTCAACCGAGTTGAGACATACATCAAGTGGCCGTGGTGGGTTTGGTTGATCATTGTTATTGTTCTCATCTTTGTTGTGTCATTACTAGTGTTCTGCTGCATTTCCACGGGTTGTTGTGGATGCTGCGGTTGCTGCGGTGCTTGTTTTTCAGGTTGTTGTAGGGGTCCTAGACTTCAACCTTACGAAGCTTGA

Protein

MRSLIYFWLLLPVLPTLSLPQDVTRCQSTTNFRRFFSKFNVQAPAVVVLGGYLPSMNSSSWYCGTGIETASGVHGIFLSYIDSSQGFEIGISQEPFDPSGYQLYLHKATNGNTNAIARLRICQFPDNKTLGPTVNDVTTGRNCLFNKAIPAYMRDGKDIVVGITWDNDRVTVFADKIYHFYLKNDWSRVATRCYNRRSCAMQYVYTPTYYMLNVTSAGEDGIYYEPCTANCTGYAANVFATDSNGHIPEGFSFNNWFLLSNDSTLLHGKVVSNQPLLVNCLLAIPKIYGLGQFFSFNHTMDGVCNGAAVDRAPEALRFNINDTSVILAEGSIVLHTALGTNLSFVCSNSSDPHLAIFAIPLGATEVPYYCFLKVDTYNSTVYKFLAVLPPTVREIVITKYGDVYVNGFGYLHLGLLDAVTINFTGHGTDDDVSGFWTIASTNFVDALIEVQGTSIQRILYCDDPVSQLKCSQVAFDLDDGFYPISSRNLLSHEQPISFVTLPLFNDHSFVNITVSAAFGGLSSANLVASDTTINGFSSFCVDTRQFTITLFYNVTNSYGYVSKSQDSNCPFTLQSVNDYLSFSKFCVSTSLLAGACTIDLFGYPAFGSGVKLTSLYFQFTKGELITGTPKPLEGITDVSFMTLDVCTKYTIYGFKGEGIITLTNSSILAGVYYTSDSGQLLAFKNVTSGAVYSVTPCSFSEQAAYVNDDIVGVISSLSNSTFNNTRELPGFFYHSNDGSNCTEPVLVYSNIGVCKSGSIGYVPSQYGQVKIAPTVTGNISIPTNFSMSIRTEYLQLYNTPVSVDCATYVCNGNSRCKQLLTQYTAACKTIESALQLSARLESVEVNSMLTISEEALQLATISSFNGDGYNFTNVLGASVYDPASGRVVQKRSVIEDLLFNKVVTNGLGTVDEDYKRCSNGRSVADLVCAQYYSGVMVLPGVVDAEKLHMYSASLIGGMALGGITAAAALPFSYAVQARLNYLALQTDVLQRNQQLLAESFNSAIGNITSAFESVKEAISQTSKGLNTVAHALTKVQEVVNSQGSALNQLTVQLQHNFQAISSSIDDIYSRLDILSADVQVDRLITGRLSALNAFVAQTLTKYTEVQASRKLAQQKVNECVKSQSQRYGFCGGDGEHIFSLVQAAPQGLLFLHTVLVPGDFVNVLAIAGLCVNGEIALTLREPGLVLFTHELQTYTATEYFVSSRRMFEPRKPTVSDFVQIESCVVTYVNLTSDQLPDVIPDYIDVNKTLDEILASLPNRTGPSLPLDVFNATYLNLTGEIADLEQRSESLRNTTEELRSLINNINNTLVDLEWLNRVETYIKWPWWVWLIIVIVLIFVVSLLVFCCISTGCCGCCGCCGACFSGCCRGPRLQPYEA

Function

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Subunit

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.

Similarity

Belongs to the alphacoronaviruses spike protein family.

Keywords

3D-structure   Coiled coil   Glycoprotein   Host membrane   Host-virus interaction   Membrane   Signal   Transmembrane   Transmembrane helix   Viral attachment to host cell   Viral envelope protein   Virion   Virulence   Virus entry into host cell  

Feature

chain  Spike glycoprotein

Uniprot

A0A2N8Z1U0   A0A2N8YGD1   A0A2N8Z1W1   Q91AV1   A0A2N8Z212   Q0GJJ0   + More
A0A2N8Z1U7   Q84712   A0A2N8Z1T8   A0A0U1YFZ0   A0A1B2RVC6   A0A0B5WYJ6   A0A0F7R7M9   F2VPY2   A2IB75   A0A286L9N9   A0A286L9M7   A0A288Y8U4   A0A286L9K9   A0A286L9L5   I1TLC3   A0A286L9N3   A0A286L9M1   A0A0F7L1H5   W6E935   F5HNC9   F5HND0   A0A0U2SJS6   F5HND1   G5D078   A0A0U3HSZ7   A0A5A4MDF7   A0A239QPZ3   A0A068BEA0   A0A3Q8TMG0   A0A2R4FC33   A0A238TMM2   A0A0H4A7A7   A0A075EAW2   A0A075EC92   A0A075ECF0   I2EBM8   A0A0H4LTY7   Q692M1   A0A109QKG8   A0A0H4J507   A0A5A4M655   A0A3G2C516   A0A2K8IYF0   A0A221HZH0   A0A0A8LEG6   A0A0A8LF79   I7FS14   A0A5A4M1T6   A0A0H4A7A3   A0A0K2D763   A0A0M4I404   A0A0S3MR49   A0A2U8QMX5   A0A0S3MR13   A0A238TN36   K4P7K4   A0A239QNQ6   A0A076MKN4   F5HND2   A0A0H4LTX6   F5HND5   A0A2R4H2S5   K4G1P9   A0A0M4HL41   A0A0M5JV19   A0A0K1LUM5   A0A0U3SAZ0   J7LKT6   A0A0H4LQ15   A0A142II42   S4V9P2   A0A223DMZ5   A0A0H4AE15   A0A089WZ38   W8Q548   A0A238TMH6   A0A0K1LUK6   A0A0K2D774   A0A3S8TLM6   S5TGV8   A0A0F6TKU6   A0A125R587   A0A219PSP4   I1TLD0   A0A0K2D7U0   A0A223DMY9   A0A0S3MSW7   J7LK74   A0A5A4M032   A0A1L3KJ30   A0A238TN08   A0A238TPF5   L7YE88   A0A239QPT4  

Pubmed

8291230    9782358    11724265    17072758    8397280    8176382    + More
28854955    22532530    26112783    21559974    21980030    27056240    25279722    26477934    29540176    26714453    29770590    25217400    29981699    28755162    24750580    25673903    26044419    26841768    27880757    23599287   

EMBL

LT905451    SNU46342.1    LT897799    SNC44845.1    LT906620    SNX30664.1    + More
AF353511    LT906581    SNW64190.1    DQ862099    ABI30278.1    LT906582    SNW64184.1    Z25483    CAA80971.1    LT905450    SNU46336.1    KJ857455    AJD09578.1    KU664503    AOC38021.1    KM887144    AJH76957.1    LC053455    BAR73378.1    GU937797    ADZ76334.1    EF185992    ABM64776.1    KX839251    AST13245.1    KY486713    KX839249    AST13130.1    AST13233.1    KX839247    AST13221.1    KY486714    AST13136.1    KX839246    AST13215.1    JQ023161    AFE85962.1    KX839250    AST13239.1    KX839248    AST13227.1    KR610991    KR610992    AKH45332.1    KF898124    AHJ11040.1    AB548618    BAK32938.1    AB548619    BAK32939.1    KR061458    ALR84908.1    AB548620    BAK32940.1    JN547228    AEQ55004.1    KU297956    ALU34112.1    MG198638    AXS76462.1    LT898439    LT900499    SNQ28038.1    SNT95659.1    KJ642641    AIC83850.1    MH061336    AZL47192.1    MF038017    AVT28076.1    LT898436    LT898435    SNQ28016.1    SNQ28045.1    KR296682    AKN45984.1    KJ645649    AID56751.1    KJ645635    KJ645702    KJ645704    LC063846    AID56667.1    AID57069.1    AID57081.1    BAT33329.1    KJ645655    AID56787.1    JQ638923    AFJ97038.1    KP698769    AKP16768.1    AY653204    AAT75298.1    KT428878    AMB20700.1    KR265761    KP688354    AKJ21713.1    AKO63237.1    MG198633    AXS76457.1    MG706122    AYM47397.1    KY619748    ATJ03603.1    KY381581    LT898447    ASM46788.1    SNQ28088.1    LM645057    CDW77205.1    LM645058    CDW77213.1    JX070672    AFP25201.1    MG198635    AXS76459.1    KR296677    AKN45979.1    KP870118    ALA15711.1    KT206205    ALD19741.1    LC063847    BAT33335.1    MH052680    AWM11503.1    LC063845    BAT33323.1    LT898445    SNQ28070.1    JX501318    AFV59240.1    LT898413    LT900502    SNQ27899.1    SNT95671.1    KJ645695    KJ645696    KM196109    KM392232    KR265759    KR265760    KM975738    KM975739    KM975740    KY619745    AID57027.1    AID57033.1    AIJ19447.1    AIN52136.1    AKJ21701.1    AKJ21707.1    AKP80610.1    AKP80617.1    AKP80624.1    ATJ03600.1    KY619780    AB548621    ATJ03635.1    BAK32941.1    KP698759    AKP16758.1    AB548624    BAK32944.1    MH006958    AVU05417.1    JQ979287    AFM55051.1    KP399614    ALD19703.1    KT206204    LT898418    LT898433    LT898441    ALD19740.1    SNQ27931.1    SNQ27997.1    SNQ28051.1    KR011122    LT898415    LT898414    LT898411    LT898412    LT898425    LT898427    LT898426    LT898438    LT898444    LT898417    AKU46227.1    SNQ27893.1    SNQ27911.1    SNQ27917.1    SNQ27955.1    SNQ27973.1    SNQ27985.1    SNQ28010.1    SNQ28022.1    SNQ28076.1    SNQ28082.1    KT388414    ALV66166.1    JX242462    AFR11480.1    KP698760    AKP16759.1    KU133258    AMR59387.1    KC787543    AGO59793.1    KY775053    ASS83140.1    KR296673    KP870138    AKN45975.1    ALA15731.1    KM403155    KM403156    KM403157    AIR95864.1    AIR95870.1    AIR95871.1    KJ399978    AHL38184.1    LT898440    SNQ28032.1    KR011121    AKU46226.1    KP870128    ALA15721.1    MH593141    AZL47242.1    KF453513    AGS46488.1    KR011756    LT898416    LT898409    LT898410    LT898430    LT898446    LT900498    AKE47378.1    SNQ27923.1    SNQ27941.1    SNQ27961.1    SNQ27991.1    SNQ28064.1    SNT95653.1    KT860508    AMD11124.1    KT591944    AMD11118.1    JQ023162    AFE85969.1    KP870125    ALA15718.1    KY775054    ASS83141.1    LC063844    BAT33317.1    JX242464    KP399632    AFR11482.1    ALD19721.1    MG198634    AXS76458.1    KX883635    APG77336.1    LT898431    SNQ27978.1    LT898408    SNQ27887.1    KC109141    KC210146    AGD98685.1    AGG55745.1    LT900500    SNT95665.1   

Proteomes

UP000281783    UP000267509    UP000276154    UP000008159    UP000269222    UP000274194    + More
UP000278337    UP000110433    UP000096210    UP000101744    UP000170862    UP000122353    UP000156820    UP000171486    UP000124407    UP000095955    UP000132054    UP000269355    UP000274124    UP000278990    UP000280596    UP000161506    UP000122852    UP000136506    UP000147669    UP000153418    UP000151262    UP000096896    UP000107726    UP000271135    UP000138211    UP000155002    UP000107825    UP000127975    UP000281631    UP000270497    UP000274353    UP000096695    UP000120524    UP000123161    UP000154382    UP000154704    UP000271078    UP000274992    UP000278554    UP000270191    UP000270545    UP000273819    UP000274834    UP000275549    UP000275656    UP000275831    UP000276052    UP000278039    UP000278495    UP000109281    UP000107011    UP000267306    UP000100204    UP000267271    UP000276298    UP000277020    UP000279022    UP000280517    UP000281047    UP000173775    UP000126260    UP000119410    UP000267609    UP000277927    UP000134644    UP000180354    UP000271448   

PRIDE

Q91AV1   

Pfam

PF01600   Corona_S1
PF01601   Corona_S2

Interpro

IPR042552   ALPHA_CORONA_SPIKE
IPR002551   Corona_S1
IPR002552   Corona_S2

ProteinModelPortal

A0A2N8Z1U0   A0A2N8YGD1   A0A2N8Z1W1   Q91AV1   A0A2N8Z212   Q0GJJ0   + More
A0A2N8Z1U7   Q84712   A0A2N8Z1T8   A0A0U1YFZ0   A0A1B2RVC6   A0A0B5WYJ6   A0A0F7R7M9   F2VPY2   A2IB75   A0A286L9N9   A0A286L9M7   A0A288Y8U4   A0A286L9K9   A0A286L9L5   I1TLC3   A0A286L9N3   A0A286L9M1   A0A0F7L1H5   W6E935   F5HNC9   F5HND0   A0A0U2SJS6   F5HND1   G5D078   A0A0U3HSZ7   A0A5A4MDF7   A0A239QPZ3   A0A068BEA0   A0A3Q8TMG0   A0A2R4FC33   A0A238TMM2   A0A0H4A7A7   A0A075EAW2   A0A075EC92   A0A075ECF0   I2EBM8   A0A0H4LTY7   Q692M1   A0A109QKG8   A0A0H4J507   A0A5A4M655   A0A3G2C516   A0A2K8IYF0   A0A221HZH0   A0A0A8LEG6   A0A0A8LF79   I7FS14   A0A5A4M1T6   A0A0H4A7A3   A0A0K2D763   A0A0M4I404   A0A0S3MR49   A0A2U8QMX5   A0A0S3MR13   A0A238TN36   K4P7K4   A0A239QNQ6   A0A076MKN4   F5HND2   A0A0H4LTX6   F5HND5   A0A2R4H2S5   K4G1P9   A0A0M4HL41   A0A0M5JV19   A0A0K1LUM5   A0A0U3SAZ0   J7LKT6   A0A0H4LQ15   A0A142II42   S4V9P2   A0A223DMZ5   A0A0H4AE15   A0A089WZ38   W8Q548   A0A238TMH6   A0A0K1LUK6   A0A0K2D774   A0A3S8TLM6   S5TGV8   A0A0F6TKU6   A0A125R587   A0A219PSP4   I1TLD0   A0A0K2D7U0   A0A223DMY9   A0A0S3MSW7   J7LK74   A0A5A4M032   A0A1L3KJ30   A0A238TN08   A0A238TPF5   L7YE88   A0A239QPT4  

PDB

6U7K     E-value=0,     Score=6556