Strain
Avian_AY319651
(Region: China; Strain: Avian infectious bronchitis virus isolate BJ, complete genome.; Date: presumed 2003)
Gene
replicase polyprotein 1a
Description
Annotated in NCBI,
replicase polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCTCCAAGCCTAAAACAGGGAGTATCTCCCCAAAACCAGAGGAGTGTCGTTCTTGTTGCCAAAGACATTCCCGATCAACTCCGTGACGCTTTGTTTTTCTATACGTCACACAACCCAAAAGATTACGCTGATGCTTTCGCTTTTAAGCAGAAGTTTGATCGTAATCTGGGCGCTGGGAAGCAGTTTAAATTTGAAACTGTGTGTGGTCCATTCTTTCTTAAAGGAGTGGATAAAGTAACACCAGGGGTCCCAGCTAAAGTTTTGAAAGCCACTTCAAAATTGGCTGATTTAGAAGACATCTTTGGTGTCTCACCTTTTGCACGGAAATACCGTGAACTTCTTAAAACAGCATGCCAGTGGTCTCTTACTGTAGATGCGCTGGATGCTCGTGCCCAAACTCTTGAAGAAATTTTTGAACCAACTGAAGTACTTTGGCTCCAGGTGGCTTCAAAACTTCAGGTTTCAGCTATGGCAATGCGCAGACTTGTTGGTGAAGTAACTGCAAAAGTCATGGATGCTTTGGGCTCAAATTTGAGTTCTCTTTTTCAAATTGTGAAACAGCAAATAGCCAGAATTTTTCAGAAAGCTCTGGCTATTTTTGAAGATGTAAAAGACTTACCACAACGCATAGCTGCACTGCAGATGGCTTTTGCAAAGTGCGCTAAAACAATTACTGTTGTTGTTGTGGAAAAAGCTATTGTTGTTCGCGAATTTTCTGGAACTTGTCTTGCAAGTATTAATGGTGCCATTGCTAAGTTCCTTGCGGAACTACCCAATGGTTTTATGGGATCTAAAGTTTTTAGCACGCTGGCTTTCTTTAAAGAGGCAGCTGTTAAAATTGTAGAGAATGTCCCAGGAGCACCGCAAGGTACTAAAGGTTTTGAAGTTGTTGGCAATGCCAAAGGCACACAAGTTGTAATTCGTGGCATGCGAAATGACTTAACACTGCTTGACCAAAAAGCTGAGATTCCTGTTGAACCAGAAGGCTGGTCTGCAATTCTGGATGGACATCTTTGTTATGTCTTTAAGAGTGGTGACAGATTTTATGCTGCGCCTTTGTCAGGAAACTTTGCACTTCATGATGTGCATTGCTGTGAACGTGTTGTCTGTCTTGTTGATGGTGTTACACCGGAGATTAATGACGGACTCATTCTTGCAGCAATCTACTCGTCATTTAGTGTGTCTGAAATTGTGGCTGCACTTAAGAAAGGTGAACCATTCAAGTTTCTTGGCCATAAGTTTGTGTATGCAAAGGAAGCTGCTGTCTCCTTCACATTGGCAAAAGCAGCTACTATTTCTGATGTCTTGAAGCTGTTTCAATCTGCTAGAGTAAAGACAGAGGATGTGTGGTCTGCACTTACTGAAAAGTCTTTTGAATTCTGGAAACTCGCATATGGAAAAGTGCGAAATCTTGAGGAATTTGTGAAGACTCATTTCTGTAAAGCCCAAATGTCAATTGTCGTTCTAGCAGTAGTGCTTGGTGAAGGCATTTGGCATCTTGTTTCTCAAGTCATCTATAGGTTGGGTGGTTTGTTTGCTAAAGTTACTGATTTTTGTGCAAAGTACTGGAAAGGTTTCTGCGAACAATTGAAAAAGGCTAAGCTTCTTGTCACAGAACACCTATGTGTCCTGAAAGGTGTTGCACAGCATTGTTTTCAACTTTTGCTGGATGCAATACATTCTATGTACAAGAGCTTTCAAAAATGTGCACTTGGTAGAATCCATGGAGATTTACTCTTCTGGAAAGGAGGTGTACACAAAGTAGTTCAGGGAGGCGACGAAATTTGGTTCGACGCCATTGACAGTATTGATGTTGAAGACCTGGGTGTCATCCAAGAAAAACCTGTAGATTTTGAGGTTTGTGATGGTATAATACTTCCAGAAAATCAACCTGGTCATATGGTCCAGTTTGAAGATGATGGAAAGAACTATATGTTCTTCCGCTTCAAACGTGAAGACAATGTTTATTATACACCAATGTCACAACTTGGTGCAATTAATGTAGTTTGCAAAGCAGGTGGCAAGACTGTCACGTTTGGTGAAACTACTGTGAAAGAAATACCACCACCTGATGTGGTGCCTATTAAGGTTAGCATCGAGTGTTGTGGTGATCCATGGAATCCAATCTTCAAGAAGGCTTATAAAGAGCCCATTGAAGTAGAAACAGACCTCACAGTAGAGCAATTGCTCACTGTGATCTACGAGAAAATGTGTGACGACCTCAAATTGTTTCCTGAGGCTCCAGAACCTCCACTATTTGAGAATGTTGCACTTGTTGATAAGAGCGGTAAAGACTTGGATTGCATAAAATCATGCCATCTCATTTACCGCGACTATGAGAGTGATGATGACATCGAAGAGGAAGACGCTGAGGAGTGCGACACAGCTTCAGAAGATGCTGAGGAGTGTGACACAAGTTCAGAGTGTGAAGAAGAGGATGAGGGTACAAAGGTCTTGGCTCTTATACAAGATCCAGCAAGTCAAAAGTACCCCCTCCCTCTTGATGATGATTACAGTGTCTATAATGGATGTATAGTCCATAAGGATGCTCTTGATGTTGTGAATCTACCATCCGGTGAAGAAACTTTTGTTATCAACAACTGCTTTGAGGGAGCTGTAAAACAACTTCCAAAGTCGGTTGTAGATGTACTTGGTGACTGGGGTGAAGCTGTTGATGCACAGGAGAAGTCCTTGAATGACGTAGCTGATTCCAGTAGTGTTTCGTTGCCGATTGAAGAGCCGGTAACAATTCATAAACAGGAATTTGTTCCTGTTGTAGAAGAAGAGCTAGATGTTATAGTTTGCACTCCAGCTGAATTAAGTGTTGTAAAGGAAGCTCCAGGAGAGGTTGACGAGTTCATCCTTATTCCAAGTGTTTCTAATGGTGAAGAAGTAGTTTCTGCTGTGGGTGCGCCACAGCAGGAAGAATCTAGAGAAGAAGTTGTTCCTGATATTTTGCAACAGGAAGAGCCTAAAATTGAAGAGCCTCCTCAAGTTGTAAAAATACAACGTGAGAGGAAAGCTAAGAAGTTCAAAGTTAAGCCAACCACACAAAAAGTTCCTAATTGTTTGGAGTATAAAACTTGTGTGGGCGATTTGACTGTTGTGATAGCCAAAGCATTGGATGAGTTTAAAGAGTTCTGCCTTGTAAACGCCGCAAATGATTATATGTCTCACGGAGGTGGCGTTGCAAAAGCAATTGCAGACTTTTGTGGTCCAGACTTTGTGGAGTATTGTGAAGACTATGTTAAGAAACATGGTCCCCAGAAAAGACTTGTCACACCTTCTTATGTTAAAGGCATTCAATGTGTGAACAATGTTGTAGGCCCACGCCATGCAGACAGTGATGTGTTTGGAAAGCTAGTTACTGCGTACAAGAATGTCATTGTTGATGGCGTAGTCAATTACGTTGTACCAGTTCTTTCATCTGGAATTTTTGGAGTTGACTTTAAGTTGTCAATAGATGCTATGCGTGTAGCTTTTGCGGATTGCAACATACGCGTTCTTCTATTTTCTCTGAACCAAGAGCATGTCGATTATTTCGACGCTACTTGCAAACAGAAGACAATCTTTCTTACAGAAGATGGTGTTAAATACCGCTCTGTTACTGTTAAACCTGGAGACTCTTTGGGTCAATTTGGACAGGTTTTTACACGACATAAGACAGTCTTTACTGCAGATGATGTTGAGGATAAAGAAATTCTCTTCATTCCAACTGCAGACAAGGCTGTCCTTGATTATTATGGTTTGGATGCGAAAAAGTATGTGATATATTTACATACGCTTTCACAAAAATGGAAAATCCTACTTAAGGACAATTTTATTCAACTTGAGTGGCGTGATGGAAATTGTTGGATTAATTCAGCAATAGTAATCCTTCAGGCTGCTAAGATTAAGTTTAAAGGTTTTCTTGAAGAAGCATGGGCTAAGCTATTGGGTGGAGATCCTACAGATTTCGTAGCATGGTGCTATGCGAGTTGCAATGCTAATGTTGGTGATTTCTCAGACACCAACTGGCTTTTGGCTAATTTGGCTGAGCATTTTGAAGTGGATTACACGAATGCACTTCTTAAGAAGCGTGTTTCTTGTAATTGTGGTGTTAAGAATTATGAGTTTAGGGGCCTTGAAGCTTGTATTCAACCAGTAAAGGCACCTAACCTGTTACATTTTAAGACACAGTACTCAAACTGTCTCACTTGTGGTTCTAATAGTGTAGATGAAGTGATAGAAGCCTCGCTGCCTTATTTGCTGCTTCTTGCTACAGATGGTCCTGCTGCAGTTGATTGTGATGAAAATGCTGTAGGAAATGTTGTTTTCATTGGGTCCACTAATAGGGGTCATTGTTATACACAAGCTGTTGGAACAGCTTTTGATAATTTGGCTCAAGATAGGAAATTTGGAAAGAAATCGCCTTACATTACTGCGATGTATACGCGCTTCTCTCTTAAAAGTAAAAACCTTATGCCTATTGCTAAGAAGAGCAAAGGTAAAAATCAAGTAGTAAAAGAAGATGTTTCTAATCTTGCTATTAGTTCAAAAGCCAGTTTTGATAATCTTACTGACTTTGAGCAGTGGTATGATAGTAACATCTATGAGAGTCTTAAAGTGCAGGAAACACCTGACAATCTTGATGAATATGTGTCATTTACAACAAAGGAGGATTCCAAGTTGCCATTGACACTTAAAGTTCGTGGTGTTAAATCAGTTGTTGATTTTAAGTCGAAAGATGGCTTTACTTATAAATTAACACCTGATATTGATGAAAATTCAAAGGCACCCGTTTATTACCCTGTCTTAGACTCTGTTAGTCTTAAGGCAATTTGGGTTGACGGAAGTGCAAACTTTGTTGTTGGACATCCAAATTATAATAGTAGAGCTTTGCGAATTCCCACTTTCTGGGAGTATGCAGAGAGTTTTGTTAAAATGGGTGAAAAAGTTAATGGTGTTACTATGGGCTTATGGCGCACAGAACACCTTAATAAACCCAATTTGGAAAATCTTTTCAACATCGTTAAGAAAATGGTTGTTGGAACTAATATTGTTACTACTCAGTGTGGTAGACTAATTGGTAAGACTGCATCATTTATAGCTGATAAAGTAGGAGATGGTGTAGTTCGTAATGTTACTGATAGAATTAAAGGGTGTTTTGGGTTTACTAGGGAGCACTTTGAAAGAAGAGTGTCTCTACAGTTCTTTAAAACCCTCTTTTTCTTCTTTTTCTGTCTTTTGAAGGCTAGTGCTAAGAGTTTAATGTCTAGCTACAAGAGTGTGCTACGTAAAGTGGTATTTACTGCTTTACTTATACTATGGTTTGTCTACACAAGCAATCCAGTTATATTTACTGGAATACGTGTGTTAGATTTCCTACTTGAAGGTTCATTTTGTAGTCCTTATGCAGATTACGGTAAAGAGTCTTTTGATATTTTACGCTATTGTGGAAGTGACTTCACCTGTCGAGTATGTTTACATGGTAAGGATTCGCTTCATTTGTATAAACATGCCTATAGCGTAGAACAGTTATACAAAGATGCAGTTAATGGCATCAGTTTTACATGGAATTGGCTTTATATGCTGTTCTTAATATTGTTTGTTAAGCCAGTGGCAGGTTTTGTTATTATTTGCTACTGTATAAGATATTTGGTTTTAAGCACAACTGTATTGCAAACTGGTGTAGGATTTTTAGACTGGTTTATTCAAACAGTGTTTGCTAACTTCAATTTTATGGGTGCAGGATTTTACTTCTGGCTCTTTTATAAGATATATATACAGGTCCATCATATAATGTACTGTAAGGATATAACATGTGAAGTGTGTAAGAAAGTTGCACGCAGTAATAGACATGAAGTTAGTGTTGTTGTTAATGGGCGTAAACAGCTTGTTCACGTATACACTAATTCTGGTTACACTTTCTGTAAGAAACATAATTGGTATTGTAAGAATTGTGACAAGTATGGTCACCAGAATACATTTATGTCTCCTGAAGTTGCTGGTGAGCTTTCAGAAAAGCTTAAACGCCATGTTAAACCTACAGCACATGCATACCACGTAGTGGATGATGCATGCTTAGTTGATGACTTTGTTAATCTGAAGTACAATGCTGCCACCCCAGGTAAAGAAGGTGTGCATTCTGCTGTTAAATGTTTTAGTGTATCTGATTTCTTAAAGAAAGCAGTCTTTCTTAAGGATGCACAAAAATGTGAACAAACAGCCACTGATAGTTTTATAGTTTGTAATACACACAGTGCGCATGCTTTGGAGGAAGCTAAAAATGCTGCCATCTATTATGCGCAATGTCTTTGTAAACCAATTCTTATACTTGACCAGGCTCTTTATGACCAATTGATTGTAGAACCTGTGTCCAAGAGTGTTGTAGACAAAGTGTGTAATATTTTGTCTAATATAATATCTGTAGATTCTGCAGCTTTGAATTATAAGGCGGGCACACTTCGTGATACCCTCCTTTCTGTCACTAAGGATGAAGAAGCTGTTGATATGGCTATTTTCTGCCATAATAATGACGTTGAATATACTACTGATGGTTTTACCAATGTGGCGCCATCATATGGTATAGACACTGGTAAGTTAACACCTCGAGATAGAGGCTTTTTGATAAATGCAGATGCTGCCATTGCTAACTTGAAAGTGAAAAATTCACCGCCAGTAGTGTGGAAGTATTCTGATCTTGTTAAACTGTCTGACAGTTGTCTTAAGTGTTTAATATCAGCAACTGTAAAATCAGGAGGTAGATTTTTCATAACTAGGTCTGGTGCTAAACAAGTTATTGCTTGTTATACTCAGAAATTGTTAGTGGAAAAGAAGGCTGGTGGTGTTATTAGTAGCACTGTTAATAGGTTTAAGAGTTTATGTAAATGGCTCTTAGTGTTTTATTTACTTTTTACAGCGTGTTGTTTGTGTTATTATCAATGGGAAATGAGTAAAGAATTTGCTCACCCCATGTATGATGTTAACTCTACAGTGCATGTAGAAGGGTTTAAGGTTATTGACAAGGGTGTACTCAGGGATATTATGCCAGAAGACACTTGTTTCTCTAATAAGTATGTAAATTCTGACTCATTTTGGGGTAAACCTTATGTAAATAGTAGAGACTGTCCTATTGTAACCGCTATTATAGATGGCGCTGGAACAGTAGCTGCTGGTGTTCCTGGTTTTGTACAGTGGGTCATGGATGGTGTAATGTTTATCCATATGACACAGACTGAGCGAAGACCGTGGTACATTCCTACTTGGTTTAATAGAGAAATTGTTGGTTATACTCATGATTCTATTGTTACAGAAGGTGAGTTTTATACTTCCATAGCATTGTTTGCTTCTAGATGTTTGTATTTAACATCTAGTAACACACCACAGCTTTACTGCTTTAATGGTGATAGCGATGCTCCTGGAGCATTACCGTTTGCAAGTATACTCCCTCATAGAGTATATTTTCAACCTAATGGAGTTAGGCTTATTGTGCCTCAGCAAATTATGCATACACCATACATAGTTAGACTTGTTTCAGATAGCTATTGTAGAGGTAGTGTTTGTGAGTATACTAAACCGGGTTATTGTGTATCACTGAACTCCCAATGGGTTTTGTTTAATGACGAATACACAAGTAAACCAGGTGTGTTTTGTGGTTCTACTGTTAGAGAACTTTTGTTTAGTATGGTTAGCACATTTTTTACGGGTGTTAATCCTAATATCTATATGCAATTGGCGACTATGTTCTTAATACTTGTTGCTGTTGTGTTAGTTCTTGCAATGGTTATAAAGTTTCAGGGTGTTTTCAAAGCTTATGCAACCACTGTATTTACTATAATGCTTGTTTGGCTTATTAACGCATTTGTATTATGTGTACATAGTTATAATAGTGTCTTAGCTATTATACTACTTGTGCTCTATTGTTATGTATCACTTGTGACGAGTCGTAATACTGCCATAGTAATGCACTGTTGGCTTGTTTTCACATTTGGTTTAATAGTACCTAATTGGATAGTTTGCGTCTATTTAGGTTTTATTCTATATATGTACACACCGCTGTTTTTGTGGTGTTATGGTACAACTAAAAATAGTCGCAAGCTTTATGAAGGCAATGAGTTTGTTGGTAATTATGATCTTGCTGCGAAGAGTACTTTTGTAATTCGTGGGCCTGAATTTGTTAAACTTACGAATGAGCTAGGCGATAAATTTGAATCCTATCTCTCAGCGTATGCTAGGCTTAAATACTACTCAGGCACTGGTAGTGAGCAAGATTACTTGCAAGCATGCCGTGCATGGTTGGCTTATGCACTAGACCAATTTAGAAGCAGTGGTGTAGAAGTAGTATACACTCCGCCACGGTATTCTATTGGTGTTAGTAGGTTACAGGCTGGTTTTAAGAAACTAGTAGCTCCTAGTAGTGCTGTTGAGAAGTGCGTTGTTAGTGTGTCCTATAGGGGTAATAATCTTAATGGATTGTGGCTGGGTGATTCTATTTACTGTCCACGTCATGTGTTAGGTAAGTTTAGTGGTGATCAATGGGATGATGTACTTAACCTTGCTAATAATCATGAGTTTGAAGTTGTAACTGGAAATGGTGTTACTTTGAATGTTGTCAGTAGGCGTTTGAGAGGTGCAGTTTTAATTTTACAAACTGCTGTTGCCAATGCTGAAACTCCAAAGTATAAGTTTATGAAAGCAAATTGTGGTGATAGTTTTACCATTGCTTGCTCTTATGGTGGTACCGTTATAGGACTCTATCCCGTTACTATGCGTTCTAATGGAACTATTAGAGCATCTTTTCTGGCAGGAGCTTGTGGCTCTGTAGGTTTTAATATAGAAAAGGGTGTAGTAAATTTCTTTTACATGCACCATTTGGAGTTACCTAATGCATTACACACTGGAACTGACTTAACGGGTGAGTTCTACGGTGGTTACATAGATGAAGAGGTTGCTCAGAAAGTTCAACCCGATAAATTGGTTACTAATAATATTTTAGCATGGCTTTATGCAGCAATTATTAGTGTTAAGGAGAGTAGTTTTTCACTGCCTAAATGGCTTGAAAGTACTACTGTTAGTGTTGATGATTATAATAAGTGGGCAGCGGATAATGGTTTTACATCATTTGTGAGCTGCACTGCTATTACTAAATTAAGTGCTATAACGGGAGTTGATGTTTGTAAACTCCTTCGCACTATTATGGTAAAAAGTACACAATGGGGTAGTGACCCTATTCTAGGACAATATAATTTTGAGGATGAGATGACACCAGAATCTGTGTTTAATCAGGTGGGTGGTGTTAGACTACAATCTTCATTTGTAAAGAAAGCTGCGTCTTGGTTTTGGAGTAGATGTGTGCTAGCTTGCTTCCTATTTGTTTTGTGTGCTATTGTGCTGTTTACAGCCATACCATATAAATATTATTTACATGGTGCTGTTGTTTTACTTGCAACTGTGCTTTTTATTTCATTTACTGTGAAACATGTTATGGCGTATATGGATACTTTTCTTTTGCCGACATTGGTTACAGTTATTATAGGAGTTTGTGTTGGAGTACCTTTCATTTACAATACTCTAATTAGTCAAATTGTTATTTTCTTTAGCCAATGGTATGATCCAGTAGTCTTTGATACTGTAGTACCGTGGATGTTCTTGCCCTTAGTCTTATATACAGCTTTTAAATGTATACAAGGTTGTTATAGCGTAAATTCCTTTAACACTTCTCTGTTATTGTTGTATCAGTTTATGAAGTTGGGTTTTGTTATATACACTTCTTCTAATACTGTTGCAGCATATTCAGAGGGTAATTGGGAATTGTTTTTTGATTTAGTTCATACTACTGTGTTGGCTAATGTTAGTAGTAATTCTTTAATTGGTTTGTTTGTCTTCAAATTGGCTAAGTGGATGCTGTATTATTGTAATGCATCATACCTTAATAATTATGTTCTAATGGCTGTCAGTGTTAATGGCATAGGTTGGATGTTCACTTGTTACTTTGGATTCTATTGGTGGATTAATAAGGTTTTCGGTTTAACCTTAGGCAAATATAATTTTAAAGTTTCAGTAGATCAATATAGGTATATGTGTCTTCATAAGATTAAACCGCCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGGATAGGTGGTGAGCGTGTGCTTCCTATAGCCACTGTTCAATCTAAATTAAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATTCTTGCCTCAGATGATGTTAATGAGTGCATGGATAACTTGTTGGGTATGCTTGTAACACTATTTTGTGTAGATAGTACTATTGATTTAAGTGAATATTGTGATGACATACTTAAGAGATCAACTGTTTTACAATCAGTTACGCAGGAGTTTTCACATATTCCTTCTTATGCAGAGTATGAAAGAGCTAAAGATCTCTATGAAAAGGTCTTAGCTGAGTCTAAAAATGGTGGTGTTACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCAATTTTTGATAGAGATTTGGCTGTTCAGAAGAAGTTGGATAGCATGGCTGAACGTGCTATGACAACAATGTATAAGGAAGCGCGTGTTACTGATAGAAGGGCGAAACTTGTTTCATCATTACATGCGCTATTATTTTCTATGCTTAAGAAGATAGATTCTGAAAAGCTTAATGTACTATTTGACCAAGCAAGCAGTGGTGTTGTGCCACTAGCTACTGTTCCAATAGTTTGTAGTAATAAGCTTACTCTTGTAGTGCCAGACCCAGAAACATGGGTCAAGTGCGTAGAGGGCATGCATGTTACATATTCAACAGTTGTTTGGAATATTGATAATGTCACTGATGCAGATGGCACTGAATTGCAACCAATTTCTATAGGTAATGGTTTAACCTATTGTATAAGTGGTGATAATATAGCATGGCCCCTTAAGGTCAATTTGACTAGGAATGCGCATAATAAAGTTGATGCAGTATTGCAGAACAACGAGCTTATGCCGCATGGTGTTAAAACAAAAGCTTGCGTTGCAGGTGTGGATCAAGCACATTGCAGCGTAGAGTCTAAGTGCTATTATACTAATATTAGTGGTAATTCAGTTGTGGCTGCTATTACTTCTTCAAATCCTAATTTGAAAGTTGCCTCATTTTTGAATGAAGCAGGCAATCAAATTTATGTTGACTTAGATCCACCATGTAAATTTGGTATGAAGGTGGGTGATAAAGTAGAAGTTGTTTACTTGTATTTTATAAAAAATACAAGGTCAATTATTAGAGGTATGGTGCTTGGTGCTATATCCAATGTTGTTGTCTTACAGTCTAAAGGGCATGAGACAGAAGAAGTGGATGCAGTTGGTATCCTCTCGCTTTGTTCCTTTGTAGTGGATCCTGCTGACACATATATTAAATATGTGGCGGCAGGTAACCAACCTTTAGGTAACTGTGTTAAAATGTTGACTGTTCATAATGGTAGTGGTTTTGCTATAACATCAAAGCCAAGTCCAACCCCTGATCAGGATTCTTATGGAGGAGCGTCTGTTTGTCTCTATTGTAGAGCACATATAGCTCATCCTGGTAGTGCTGGAAACTTAGATGGACGCTGTCAATTTAAGGGAAGTTTTGTGCAAATACCTACTACGGAGAAAGACCCTGTCGGCTTCTGTCTGCGTAATAAGGTTTGCACTGTGTGTCAGTGTTGGATTGGGCATGGCTGTCAATGTGATGCACTTAGACAACCTAAACCATCTGTTCAATCAGCTGCTGGTGCAACAAATTTTGATAAGAATTATTTAAACGGGTACGGGGTAACAGTGAGGCTCGGCTGA
Protein
MAPSLKQGVSPQNQRSVVLVAKDIPDQLRDALFFYTSHNPKDYADAFAFKQKFDRNLGAGKQFKFETVCGPFFLKGVDKVTPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVDALDARAQTLEEIFEPTEVLWLQVASKLQVSAMAMRRLVGEVTAKVMDALGSNLSSLFQIVKQQIARIFQKALAIFEDVKDLPQRIAALQMAFAKCAKTITVVVVEKAIVVREFSGTCLASINGAIAKFLAELPNGFMGSKVFSTLAFFKEAAVKIVENVPGAPQGTKGFEVVGNAKGTQVVIRGMRNDLTLLDQKAEIPVEPEGWSAILDGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLVDGVTPEINDGLILAAIYSSFSVSEIVAALKKGEPFKFLGHKFVYAKEAAVSFTLAKAATISDVLKLFQSARVKTEDVWSALTEKSFEFWKLAYGKVRNLEEFVKTHFCKAQMSIVVLAVVLGEGIWHLVSQVIYRLGGLFAKVTDFCAKYWKGFCEQLKKAKLLVTEHLCVLKGVAQHCFQLLLDAIHSMYKSFQKCALGRIHGDLLFWKGGVHKVVQGGDEIWFDAIDSIDVEDLGVIQEKPVDFEVCDGIILPENQPGHMVQFEDDGKNYMFFRFKREDNVYYTPMSQLGAINVVCKAGGKTVTFGETTVKEIPPPDVVPIKVSIECCGDPWNPIFKKAYKEPIEVETDLTVEQLLTVIYEKMCDDLKLFPEAPEPPLFENVALVDKSGKDLDCIKSCHLIYRDYESDDDIEEEDAEECDTASEDAEECDTSSECEEEDEGTKVLALIQDPASQKYPLPLDDDYSVYNGCIVHKDALDVVNLPSGEETFVINNCFEGAVKQLPKSVVDVLGDWGEAVDAQEKSLNDVADSSSVSLPIEEPVTIHKQEFVPVVEEELDVIVCTPAELSVVKEAPGEVDEFILIPSVSNGEEVVSAVGAPQQEESREEVVPDILQQEEPKIEEPPQVVKIQRERKAKKFKVKPTTQKVPNCLEYKTCVGDLTVVIAKALDEFKEFCLVNAANDYMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQKRLVTPSYVKGIQCVNNVVGPRHADSDVFGKLVTAYKNVIVDGVVNYVVPVLSSGIFGVDFKLSIDAMRVAFADCNIRVLLFSLNQEHVDYFDATCKQKTIFLTEDGVKYRSVTVKPGDSLGQFGQVFTRHKTVFTADDVEDKEILFIPTADKAVLDYYGLDAKKYVIYLHTLSQKWKILLKDNFIQLEWRDGNCWINSAIVILQAAKIKFKGFLEEAWAKLLGGDPTDFVAWCYASCNANVGDFSDTNWLLANLAEHFEVDYTNALLKKRVSCNCGVKNYEFRGLEACIQPVKAPNLLHFKTQYSNCLTCGSNSVDEVIEASLPYLLLLATDGPAAVDCDENAVGNVVFIGSTNRGHCYTQAVGTAFDNLAQDRKFGKKSPYITAMYTRFSLKSKNLMPIAKKSKGKNQVVKEDVSNLAISSKASFDNLTDFEQWYDSNIYESLKVQETPDNLDEYVSFTTKEDSKLPLTLKVRGVKSVVDFKSKDGFTYKLTPDIDENSKAPVYYPVLDSVSLKAIWVDGSANFVVGHPNYNSRALRIPTFWEYAESFVKMGEKVNGVTMGLWRTEHLNKPNLENLFNIVKKMVVGTNIVTTQCGRLIGKTASFIADKVGDGVVRNVTDRIKGCFGFTREHFERRVSLQFFKTLFFFFFCLLKASAKSLMSSYKSVLRKVVFTALLILWFVYTSNPVIFTGIRVLDFLLEGSFCSPYADYGKESFDILRYCGSDFTCRVCLHGKDSLHLYKHAYSVEQLYKDAVNGISFTWNWLYMLFLILFVKPVAGFVIICYCIRYLVLSTTVLQTGVGFLDWFIQTVFANFNFMGAGFYFWLFYKIYIQVHHIMYCKDITCEVCKKVARSNRHEVSVVVNGRKQLVHVYTNSGYTFCKKHNWYCKNCDKYGHQNTFMSPEVAGELSEKLKRHVKPTAHAYHVVDDACLVDDFVNLKYNAATPGKEGVHSAVKCFSVSDFLKKAVFLKDAQKCEQTATDSFIVCNTHSAHALEEAKNAAIYYAQCLCKPILILDQALYDQLIVEPVSKSVVDKVCNILSNIISVDSAALNYKAGTLRDTLLSVTKDEEAVDMAIFCHNNDVEYTTDGFTNVAPSYGIDTGKLTPRDRGFLINADAAIANLKVKNSPPVVWKYSDLVKLSDSCLKCLISATVKSGGRFFITRSGAKQVIACYTQKLLVEKKAGGVISSTVNRFKSLCKWLLVFYLLFTACCLCYYQWEMSKEFAHPMYDVNSTVHVEGFKVIDKGVLRDIMPEDTCFSNKYVNSDSFWGKPYVNSRDCPIVTAIIDGAGTVAAGVPGFVQWVMDGVMFIHMTQTERRPWYIPTWFNREIVGYTHDSIVTEGEFYTSIALFASRCLYLTSSNTPQLYCFNGDSDAPGALPFASILPHRVYFQPNGVRLIVPQQIMHTPYIVRLVSDSYCRGSVCEYTKPGYCVSLNSQWVLFNDEYTSKPGVFCGSTVRELLFSMVSTFFTGVNPNIYMQLATMFLILVAVVLVLAMVIKFQGVFKAYATTVFTIMLVWLINAFVLCVHSYNSVLAIILLVLYCYVSLVTSRNTAIVMHCWLVFTFGLIVPNWIVCVYLGFILYMYTPLFLWCYGTTKNSRKLYEGNEFVGNYDLAAKSTFVIRGPEFVKLTNELGDKFESYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQFRSSGVEVVYTPPRYSIGVSRLQAGFKKLVAPSSAVEKCVVSVSYRGNNLNGLWLGDSIYCPRHVLGKFSGDQWDDVLNLANNHEFEVVTGNGVTLNVVSRRLRGAVLILQTAVANAETPKYKFMKANCGDSFTIACSYGGTVIGLYPVTMRSNGTIRASFLAGACGSVGFNIEKGVVNFFYMHHLELPNALHTGTDLTGEFYGGYIDEEVAQKVQPDKLVTNNILAWLYAAIISVKESSFSLPKWLESTTVSVDDYNKWAADNGFTSFVSCTAITKLSAITGVDVCKLLRTIMVKSTQWGSDPILGQYNFEDEMTPESVFNQVGGVRLQSSFVKKAASWFWSRCVLACFLFVLCAIVLFTAIPYKYYLHGAVVLLATVLFISFTVKHVMAYMDTFLLPTLVTVIIGVCVGVPFIYNTLISQIVIFFSQWYDPVVFDTVVPWMFLPLVLYTAFKCIQGCYSVNSFNTSLLLLYQFMKLGFVIYTSSNTVAAYSEGNWELFFDLVHTTVLANVSSNSLIGLFVFKLAKWMLYYCNASYLNNYVLMAVSVNGIGWMFTCYFGFYWWINKVFGLTLGKYNFKVSVDQYRYMCLHKIKPPKTVWEVFSTNILIQGIGGERVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVNECMDNLLGMLVTLFCVDSTIDLSEYCDDILKRSTVLQSVTQEFSHIPSYAEYERAKDLYEKVLAESKNGGVTQQELAAYRKAANIAKSIFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVVPDPETWVKCVEGMHVTYSTVVWNIDNVTDADGTELQPISIGNGLTYCISGDNIAWPLKVNLTRNAHNKVDAVLQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIIRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFVVDPADTYIKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGHGCQCDALRQPKPSVQSAAGATNFDKNYLNGYGVTVRLG
Summary
Function
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Subunit
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Membrane
Metal-binding
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
EMBL
Proteomes
Pfam
Interpro
IPR018995
RNA_synth_NSP10_coronavirus
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR036499 NSP9_sf
IPR014829 NSP8
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR002589 Macro_dom
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR040795 Nsp1a_N
IPR036333 NSP10_sf
IPR032505 Corona_NSP4_C
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
SUPFAM
Gene 3D
ProteinModelPortal
PDB
3LD1
E-value=0.0 Score= 622 Identity=86.03%
Cov(Q)=9.03% Cov(P)=99.72%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0008242 F:omega peptidase activity
GO:0016021 C:integral component of membrane
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039520 P:induction by virus of host autophagy
GO:0033644 C:host cell membrane
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
3963
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
417.038559999998
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00674
outside
1 - 1761
TMhelix
1762 - 1784
inside
1785 - 1859
TMhelix
1860 - 1882
outside
1883 - 1896
TMhelix
1897 - 1919
inside
1920 - 2291
TMhelix
2292 - 2311
outside
2312 - 2372
TMhelix
2373 - 2395
inside
2396 - 2568
TMhelix
2569 - 2591
outside
2592 - 2595
TMhelix
2596 - 2618
inside
2619 - 2624
TMhelix
2625 - 2647
outside
2648 - 2661
TMhelix
2662 - 2684
inside
2685 - 3106
TMhelix
3107 - 3129
outside
3130 - 3133
TMhelix
3134 - 3151
inside
3152 - 3163
TMhelix
3164 - 3186
outside
3187 - 3200
TMhelix
3201 - 3223
inside
3224 - 3235
TMhelix
3236 - 3258
outside
3259 - 3267
TMhelix
3268 - 3290
inside
3291 - 3309
TMhelix
3310 - 3332
outside
3333 - 3963
