Strain
Cow_FJ938066
(Region: USA: Ohio; Strain: Bovine respiratory coronavirus bovine/US/OH-440-TC/1996, complete genome.; Date: 1-Jan-96)
Gene
orf1a polyprotein
Description
Annotated in NCBI,
orf1a polyprotein
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGTCGAAGATCAACAAATACGGTCTCGAACTACACTGGGCTCCAGAATTTCCATGGATGTTTGAGGACGCAGAGGAGAAGTTGGACAACCCTAGTAGTTCAGAGGTGGATATAGTATGCTCCACCACTGCGCAAAAGCTGGAAACAGGCGGAATTTGTCCTGAAAATCATGTGATGGTGGATTGTCGCCGACTTCTTAAACAAGAATGTTGTGTGCAGTCTAGCCTAATACGTGAAATTGTTATGAATACACGTCCATATGATTTGGAGGTGCTACTTCAAGATGCTTTGCAGTCCCGCGAAGCAGTTTTGGTTACACCCCCTCTAGGTATGTCTCTGGAGGCATGCTATGTGAGAGGTTGTAATCCTAATGGATGGACCATGGGTTTGTTTCGGCGTAGAAGTGTGTGTAACACTGGTCGCTGCGCTGTTAACAAGCATGTGGCCTATCAGCTATATATGATTGATCCTGCGGGTGTCTGTTTTGGTGCAGGTCAATTTGTGGGTTGGGTTATACCCTTAGCCTTTATGCCTGTGCAATCCCGGAAATTTATTGTTCCTTGGGTTATGTACTTGCGTAAGTGTGGCGAAAAGGGTGCCTACAACAAAGATCATAAACGTGGCGGTTTTGAACACGTTTATAATTTTAAAGTTGAGGATGCTTACGACTTGGTTCATGATGAGCCTAAGGGTAAGTTTTCTAAGAAGGCTTATGCTTTAATTAGAGGATACCGTGGTGTTAAACCGCTTCTTTATGTAGACCAGTATGGTTGTGATTATACTGGTGGTCTTGCAGATGGCTTAGAGGCTTATGCTGATAAGACATTGCAAGAAATGAAGGCATTATTTCCTGTTTGGAGCCAGGAACTCCCTTTTGATGTAACTGTGGCATGGCACGTTGTGCGTGATCCACGTTATGTTATGAGACTGCAGAGTGCTTCTACTATACGTAGTGTTGCATATGTTGCTAACCCTACTGAAGACTTGTGTGATGGTTCTGTTGTTATAAAGGAACCTGTGCATGTTTATGCGGATGACTCTATTATTTTACGTCAACATAATTTAGTTGACATTATGAGTTGTTTTTATATGGAGGCAGATGCAGTTGTAAATGCTTTTTATGGTGTTGATTTGAAAGATTGTGGTTTTGTTATGCAGTTTGGTTATATTGACTGCGAACAAGACTTGTGTGATTTTAAAGGTTGGGTTCCTGGTAATATGATAGATGGTTTTGCTTGCACTACTTGTGGTCATGTTTATGAGACAGGTGATTTGCTAGCACAATCTTCAGGTGTTCTGCCTGTTAATCCTGTATTGCATACTAAGAGTGCAGCAGGTTATGGTGGTTTTGGTTGTAAGGATTCTTTTACCCTGTATGGCCAAACTGTAGTTTATTTTGGAGGTTGTGTGTATTGGAGTCCAGCACGTAATATATGGATTCCTATATTAAAATCTTCTGTTAAGTCTTATGACGGTTTGGTTTATACTGGAGTTGTAGGTTGCAAGGCTATTGTAAAGGAAACAAATCTCATTTGCAAAGCGTTGTACCTTGATTATGTTCAACACAAGTGTGGCAATTTACACCAGCGGGAGTTGCTAGGTGTGTCAGATGTGTGGCATAAACAATTGTTATTAAATAGAGGGGTGTACAAACCTCTTTTAGAGAATATTGATTATTTTAATATGCGGCGCGCTAAATTTAGTTTAGAAACTTTTACTGTTTGTGCAGATGGTTTTATGCCTTTTCTTTTAGATGATTTGGTTCCGCGCGCATATTATTTGGCAGTAAGTGGTCAAGCATTTTGTGACTACGCAGATAAAATCTGCCATGCTGTTGTGTCTAAGAGTAAAGAGTTACTTGATGTGTCTCTGGATTCTTTAAGTGCAGCTATACATTATTTGAATTCTAAAATTGTTGATTTGGCTCAACATTTTAGTGATTTTGGAACAAGTTTTGTTTCTAAAATTGTTCATTTCTTTAAGACTTTTACTACTAGCACTGCTCTTGCATTTGCATGGGTTTTATTTCATGTTTTGCATGGTGCTTATATAGTAGTAGAGAGTGATATATATTTTGTTAAAAACATTCCTCGTTATGCTAGTGCTGTTGCACAAGCATTTCGGAGTGTTGCTAAAGTTGTACTGGACTCTTTAAGAGTTACTTTTATTGATGGCCTTTCTTGTTTTAAGATTGGACGTAGAAGAATTTGTCTCTCAGGCAGTAAAATTTATGAAGTTGAGCGTGGCTTGTTACATTCATCTCAATTGCCATTAGATGTTTATGATTTAACCATGCCTAGTCAAGTTCAGAAAGCCAAGCAAAAACCTATTTATTTAAAAGGTTCTGGTTCTGATTTTTCATTAGCAGATAGTGTGGTTGAAGTTGTTACAACTTCACTTACACCATGTGGTTATTCTGAACCACCTAAAGTTGCAGATAAAATTTGCATTGTGGATAATGTTTATATGGCCAAGGCTGGTGACAAATATTACCCTGTTGTGGTTGATGGTCAAGTAGGACTCTTGGATCAAGCATGGAGGGTTCCTTGTGCTGGAAGGCGTGTTACATTTAAGGAACAGCCTACAGTAAATGAGATTGCAAGCACACCTAAGACTATTAAAGTTTTTTATGAGCTTGACAAAGATTTTAATACTATTTTAAACACTGCATGTGGAGTGTTTGAAGTAGACGATACCGTGGATATGGAGGAATTTTATGCTGTGGTGGTTGATGCCATAGAAGAGAAACTTTCTCCATGTAAGGAGCTTGAAGGTGTAGGTGCTAAAGTTAGTGCCTTTTTACAGAAATTAGAGGATAATTCCCTATTTTTATTTGATGAGGCTGGCGAGGAAGTTCTTGCTCCTAAATTGTATTGTGCTTTTACAGCTCCTGAAGATGATGACTTTCTTGAAGAAAGTGGTGTTGAAGAAGATGATGTAGAAGGTGAGGAAACTGATTTAACTGTCACAAGTGCTGGAGAGCCTTGTGTTGCCAGTGAACAGGAGGAGTCTTCTGAAATCTTAGAGGACACTTTGGATGATGGTCCATGTGTGGAGACATCTGATTCACAAGTTGAAGAAGATGTAGAAATGTCGGATTTTGTTGATCTTGAATCTGTGATTCAGGATTATGAAAATGTTTGTTTTGAGTTTTATACTACAGAGCCAGAATTTGTTAAAGTTTTGGATCTGTATGTGCCTAAAGCAACTCGCAACAATTGCTGGTTGCGATCAGTTTTGGCAGTGATGCAGAAACTGCCCTGTCAATTTAAAGATAAAAATTTGCAGGATCTTTGGGTGTTATATAAGCAACAGTATAGTCAGTTGTTTGTTGATACCTTGGTTAATAAGATACCTGCTAATATTGTAGTTCCACAAGGTGGTTATGTTGCCGATTTTGCATATTGGTTCTTAACCTTATGTGATTGGCAGTGTGTTGCATATTGGAAATGCATTAAATGTGATTTAGCTCTTAAGCTTAAAGGCTTGGATGCTATGTTCTTTTATGGTGATGTTGTCTCACATGTGTGCAAGTGTGGTGAGTCTATGGTACTTATTGATGTTGATGTGCCATTTACAGCCCACTTTGCTCTTAAAGATAAGTTGTTTTGTGCATTTATTACTAAGCGTAGTGTGTATAAAGCAGCTTGTGTTGTGGATGTTAATGATAGTCATTCTATGGCTGTTGTTGATGGTAAACAAATTGATGATCATCGTGTCACTAGTATTACTAGTGATAAGTTTGATTTTATTATTGGGCATGGTATGTCATTTTCAATGACTACTTTCGAAATTGCCCAATTGTATGGTTCTTGTATAACACCTAATGTATGTTTTGTTAAAGGTGATATAATTAAAGTTTCTAAGCGTGTTAAAGCAGAAGTCGTTGTAAATCCTGCTAATGGCCATATGGCACATGGTGGTGGTGTTGCAAAGGCTATTGCAGTAGCAGCTGGACAGCAGTTTGTTAAAGAGACTACCGATATGGTTAAGTCTAAAGGAGTTTGTGCTACTGGAGACTGTTATGTCTCTACAGGGGGTAAATTATGTAAAACTGTGCTTAATGTTGTTGGACCTGATGCGAGAACACAGGGTAAACAAAGTTATGCATTGTTAGAGCGTGTTTATAAACATCTTAACAAATATGATTGTGTTGTTACAACTTTGATATCAGCTGGTATATTTAGTGTGCCTTCTGATGTGTCTTTAACATATCTACTTGGTACTGCCGAGAAACAAGTTGTTCTTGTTAGCAATAATCAAGAGGATTTTGATCTTATTTCTAAGTGTCAGATAACTGCCGTTGAGGGCACTAAGAAATTGGCAGAGCGTCTTTCTTTTAATGTAGGGCGTTCTATCGTTTACGAAACAGATGCTAATAAGTTGATTTTAAGCAATGACGTTGCATTTGTTTCGACATTTAATGTCTTACAGGATGTTTTATCCTTAAGACATGATATAGCACTTGATGATGATGCACGAACCTTTGTTCAGAGCAATGTTGATGTTGTACCTGAGGGTTGGCGTGTTGTCAATAAGTTTTATCAAATTAATGGTGTTAGAACCGTTAAGTATTTTGAGTGTCCCGGTGGCATAGATATATGCAGCCAGGATAAAGTTTTTGGTTATGTACAGCAGGGTAGTTTTAATAAGGCTACTGTTGCTCAAATTAAAGCCTTGTTTTTGGATAAAGTGGACATCTTGCTAACTGTTGATGGTGTCAATTTCACTAACAGGTTTGTGCCTGTAGGTGAAAGTTTTGGTAAGAGTCTAGGAAATGTGTTCTGTGATGGAGTTAATGTCACGAAACATAAGTGTGATATAAATTATAAAGGTAAAGTCTTTTTCCAGTTTGATAATCTTTCTAGTGAAGATTTAAAGGCTGTAAGAAGTTCTTTTAATTTTGATCAGAAGGAATTGCTTGCCTACTACAACATGCTTGTTAATTGTTCTAAATGGCAGGTTGTTTTTAATGGTAAGTATTTCACTTTTAAGCAAGCTAATAACAATTGTTTTGTTAATGTTTCTTGCTTAATGCTCCAGAGTTTGAATCTGAAATTTAAAATTGTTCAATGGCAGGAGGCGTGGCTTGAATTTCGTTCTGGCCGCCCTGCTAGATTTGTATCTTTGGTTTTAGCCAAAGGTGGGTTTAAATTTGGTGATCCTGCTGATTCTAGAGATTTCTTGCGTGTTGTGTTTAGTCAAGTTGATTTGACAGGGGCAATATGTGATTTTGAAATTGCATGTAAATGTGGTGTAAAGCAGGAACAGCGTACTGGTGTGGACGCTGTTATGCATTTTGGTACATTGAGTCGTGAAGATCTTGAGATTGGTTACACCGTGGATTGTTCTTGCGGTAAAAAGCTAATCCATTGTGTACGATTTGATGTACCATTTTTAATTTGTAGTAATACACCTGCTAGTGTAAAATTACCTAAGGGTGTAGGAAGTGCAAATATTTTTAAAGGTGATAAGGTTGGTCATTATGTTCATGTTAAGTGTGAACAGTCTTATCAGCTTTATGATGCTTCTAATGTTAAGAAGGTTACAGACGTTACTGGCAATTTGTCAGATTGTTTGTATCTTAAAAATTTGAAACAAACTTTTAAATCGGTGTTAACCACCTATTATTTGGATGATGTTAAGAAAATTGAGTATAACCCTGACTTGTCACAATATTATTGTGACGGAGGTAAGTATTATACTCAGCGTATTATTAAAGCCCAATTTAAAACATTTGAGAAAGTAGATGGTGTGTATACTAATTTTAAATTGATAGGACACACCATCTGTGATATTCTTAATGCTAAGTTGGGTTTTGATAGCTCTAAAGAGTTTGTTGAATATAAAGTTACTGAGTGGCCAACAGCTACAGGTGATGTGGTGTTGGCTACTGATGATTTGTATGTTAAGAGATACGAAAGGGGTTGTATTACTTTTGGTAAACCTGTTATATGGTTAAGCCATGAGCAAGCTTCCCTCAATTCTTTAACATATTTTAATAGACCTTTATTGGTTGATGAGAATAAATTTGATGTTTTAAAAGTGGATGATGTTGACGATGGTGGTGATATCTCAGAGAGTGATGCCAAAGAATCCAAAGAAATCAACATTATTAAGTTAAGTGGTGTTAAAAAACCATTTAAGGTTGAAGATAGTGTCATTGTTAATGATGATACTAGTGAAATCAAATATGTTAAGAGTTTGTCTATAGTTGATGTGTATGATATGTGGCTTACAGGTTGTAGGTATGTTGTTAGGACTGCTAATGCTTTGAGCATGGCAGTTAACGTACCTACAATACGTAAGTTTATAAAATTTGGTATGACTATTGTTAGTATACCAATTGATTTGTTAAATTTAAGAGAGATTAAGCCTGTTTTTAATGTGGTTAAAGCTGTGCGAAATAAAATTTCTGCATGCTTTAATTTTATTAAATGGCTTTTTGTCTTATTATTTGGCTGGATTAAAATATCCGCTGATAATAAAGTAATTTATACCACAGAAGTTGCATCAAAGCTTACATGTAAGCTTGTAGCTTTAGCTTTTAAAAATGCGTTTTTGACATTTAAGTGGAGTGTGGTTGCTAGAGGTGCTTGCATTATAGCGACTATATTTCTATTGTGGTTTAATTTTATATATGCCAATGTAATTTTTAGTGATTTTTATTTGCCTAAAATTGGTTTCTTGCCGACTTTTGTTGGTAAGATCGCACAGTGGATTAAGAGCACTTTTAGTCTTGTAACTATTTGTGATCTATATTCCATTCAGGATGTGGGTTTTAAGAATCAGTATTGTAATGGAAGTATCGCATGTCAATTCTGCTTGGCAGGATTTGATATGTTAGATAATTATAAAGCCATTGATGTAGTACAGTATGAAGCTGATAGGAGAGCATTTGTTGATTATACAGGTGTGTTAAAGATTGTCATTGAATTGATAGTTAGTTACGCCCTGTATACGGCATGGTTCTACCCATTGTTTGCTCTTATTAGTATTCAGATCTTGACCACTTGGCTGCCTGAGCTTTTTATGCTTAGTACATTACATTGGAGTGTTAGGTTGCTGGTGTCTTTAGCTAATATGTTACCAGCACATGTGTTTATGAGGTTTTATATTATTATTGCCTCTTTTATTAAGCTGTTTAGCTTGTTTAGGCATGTTGCCTATGGTTGTAGTAAACCTGGTTGTTTGTTTTGTTACAAGAGGAATCGTAGTCTACGTGTTAAATGTAGTACTATTGTTGGTGGCATGATACGCTATTACGATGTTATGGCTAATGGTGGCACTGGCTTTTGTTCAAAACATCAATGGAATTGCATTGATTGTGATTCTTATAAACCAGGTAATACTTTTATTACTGTTGAGGCCGCTCTTGATTTATCTAAGGAATTGAAACGGCCTATTCAGCCTACAGATGTTGCTTATCATACGGTTACGGATGTTAAGCAAGTTGGTTGTTATATGCGCTTGTTCTATGAGCGTGATGGACAGCGCACATATGATGATGTTAATGCTAGTTTGTTTGTGGATTATAGTAATTTGCTACATTCTAAGGTTAAGGGTGTGCCTAATATGCATGTTGTGGTAGTGGAAAATGATGCCGATAAAGCTAATTTTCTTAATGCTGCTGTATTTTATGCACAGTCTTTGTTTAGACCTATTTTAATGGTTGATAAAAATCTGATAACTACTGCTAATACTGGTACGTCTGTTACAGAAACTATGTTTGATGTTTATGTTGACACATTTTTGTCTATGTTTGATGTGGATAAAAAGAGTCTTAATGCTTTAATAGCAACTGCGCATTCTTCTATAAAACAGGGTACGCAGATTTGTAAAGTTTTGGATACCTTTTTAAGCTGTGCTCGTAAAAGTTGTTCTATTGATTCAGATGTTGATACTAAGTGTTTAGCTGATTCTGTCATGTCTGCTGTATCGGCAGGCCTTGAATTGACGGATGAAAGTTGTAATAACTTGGTGCCAACATATTTGAAGGGTGATAACATTGTGGCAGCTGATTTAGGTGTTCTGATTCAAAATTCTGCTAAGCATGTGCAGGGTAATGTTGCTAAAATAGCCGGTGTTTCCTGTATATGGTCTGTGGATGCTTTTAATCAGCTAAGTTCTGATTTCCAGCATAAATTGAAGAAAGCATGTTGTAAAACTGGTTTGAAATTGAAGCTTACTTATAATAAGCAGATGGCTAATGTCTCTGTTTTAACTACACCCTTTAGTCTTAAAGGGGGTGCAGTTTTTAGTTATTTTGTTTATGTATGTTTTCTGTTGAGTTTGGTTTGTTTTATTGGATTGTGGTGCTTAATGCCCACTTACACAGTACACAAATCAGATTTTCAGCTTCCCGTTTATGCCAGTTATAAAGTTTTAGATAATGGTGTTATTAGAGATGTTAGCGTTGAAGATGTTTGTTTCGCTAACAAATTTGAACAATTTGATCAATGGTATGAGTCTACATTTGGTCTAAGTTATTATAGTAACAGTATGGCTTGTCCCATTGTTGTTGCTGTAGTAGACCAGGATCTTGGCTCTACTGTGTTTAATGTCCCTACCAAAGTGTTACGATATGGTTACCATGTGTTGCACTTTATTACACATGCACTTTCTGCTGATGGAGTGCAGTGTTATACGCCACATAGTCAAATATCGTATTCTAATTTTTATGCTAGTGGCTGTGTGCTTTCCTCCGCTTGTACTATGTTTGCAATGGCCGATGGTAGTCCACAACCTTATTGTTATACAGAGGGGCTTATGCAGAACGCTTCTCTGTATAGTTCATTGGTACCTCACGTGCGGTATAATCTTGCTAATGCTAAAGGTTTTATCCGTTTTCCAGAAGTGTTGCGAGAAGGACTTGTGCGTATTGTGCGTACTCGTTCTATGTCGTATTGCAGAGTTGGATTATGTGAGGAAGCTGATGAGGGTATATGCTTTAACTTTAATGGTTCTTGGGTGCTTAATAATGATTATTATAGATCATTGCCTGGGACCTTTTGTGGTAGAGATGTTTTTGACTTAATTTATCAGCTTTTTAAAGGTTTAGCACAGCCTGTGGATTTCTTGGCATTGACTGCTAGTTCTATTGCTGGTGCTATACTTGCTGTAATTGTTGTTTTGGTGTTTTATTACTTAATAAAGCTTAAACGTGCTTTTGGTGATTACACCAGTATTGTTTTTGTCAATGTGATTGTGTGGTGTGTAAATTTTATGATGCTTTTTGTGTTTCAAGTTTACCCTACACTTTCTTGTGTATATGCTATTTGTTATTTTTATGCCACGCTTTATTTCCCTTCGGAGATAAGTGTGATAATGCATTTACAATGGCTAGTTATGTATGGCACTATTATGCCTTTATGGTTTTGTTTGCTATATATATCTGTTGTTGTTTCAAATCATGCTTTTTGGGTATTCGCTTACTGCAGACGGCTTGGTACTTCTGTTCGTAGTGATGGTACATTTGAAGAAATGGCTCTTACTACTTTTATGATTACAAAAGATTCTTATTGTAAGCTTAAGAATTCTTTGTCTGATGTTGCTTTTAATAGATATTTGAGTTTGTATAATAAATATAGGTATTACAGCGGTAAAATGGATACTGCTGCATATAGGGAGGCTGCTTGTTCTCAGTTGGCTAAAGCAATGGATACATTTACCAATAATAATGGTAGTGATGTGCTTTACCAACCACCTACTGCTTCCGTTTCAACTTCATTCTTGCAATCTGGTATTGTGAAAATGGTTAATCCTACTTCTAAGGTAGAACCATGTATTGTCAGTGTTACCTATGGTAATATGACATTGAATGGTTTATGGTTGGATGATAAGGTCTACTGTCCCAGACATGTGATATGTTCTGCTTCAGATATGACTAATCCAGATTATACAAATTTGTTGTGTAGAGTAACATCAAGTGATTTTACTGTATTGTTTGATCGTTTAAGCCTTACAGTGATGTCTTATCAAATGCAGGGTTGTATGCTTGTTCTTACAGTGACCCTGCAAAATTCTCGTACGCCAAAATATACATTTGGTGTGGTTAAACCTGGTGAGACTTTTACTGTTTTAGCTGCTTATAACGGCAAACCACAAGGAGCCTTTCATGTGACTATGCGTAGTAGTTATACCATTAAGGGTTCCTTTTTATGCGGATCTTGTGGATCTGTTGGTTATGTGCTAATGGGTGATTGTGTTAAATTTGTGTATATGCATCAATTGGAGCTTAGTACTGGTTGTCATACTGGTACTGATTTCAATGGGGATTTTTATGGTCCTTATAAGGATGCTCAGGTTGTCCAATTGCCCGTTCAGGATTATATACAATCTGTTAATTTTGTAGCATGGCTTTATGCTGCTATACTTAATAATTGTAATTGGTTTGTACAAAGTGATAAGTGTTCTGTTGAAGATTTTAATGTGTGGGCTTTGTCTAATGGGTTTAGCCAAGTTAAGTCTGATCTTGTTATAGATGCTTTAGCTTCTATGACTGGTGTGTCTTTGGAAACACTATTGGCTGCTATTAAGCGTCTCAAGAATGGTTTTCAAGGACGTCAGGTTATGGGTAGTTGCTCCTTTGAGGATGAATTGACACCTAGCGATGTTTATCAACAACTCGCTGGTATCAAGTTACAATCAAAGCGTACTAGATTGGTTAAAGGCATTGTTTGTTGGATTATGGCTTCTACATTTTTGTTTAGTTGTATAATTACAGCATTTGTGAAATGGACTATGTTTATGTATGTAACTACTAATATGCTTAGTATTACGTTTTGTGCACTTTGTGTTATAAGTTTGGCCATGTTGTTGGTTAAACATAAGCATCTTTATTTGACTATGTATATAATTCCTGTGCTTTTTACACTGCTGTATAACAACTATTTGGTTGTGTACAAGCAGACATTTAGAGGCTATGTTTATGCATGGCTATCATATTATGTTCCATCAGTTGAGTATACTTATACTGATGAAGTTATTTATGGCATGTTATTGCTTATAGGAATGGTCTTTGTTACATTACGTAGCATTAACCATGATTTGTTCTCTTTTATAATGTTTGTTGGCCGTGTGATTTCTGTTGTCTCTTTGTGGTACATGGGTTCTAACTTAGAGGAAGAAATTCTTCTTATGTTGGCTTCTCTTTTTGGTACTTACACATGGACAACAGCTTTATCTATGGCTGCAGCAAAGGTTATTGCTAAGTGGGTTGCTGTGAATGTTTTGTATTTCACAGATATACCTCAAATTAAGATAGTGCTTGTATGCTATTTGTTTATAGGTTATATTATTAGCTGTTATTGGGGTTTGTTTTCCTTGATGAACAGTTTGTTTAGAATGCCTTTGGGTGTTTATAATTATAAAATTTCAGTACAGGAATTAAGATATATGAATGCTAATGGATTGCGCCCTCCTAAGAATAGTTTTGAAGCCCTTATGCTTAATTTTAAGCTTTTGGGTATTGGAGGTGTGCCAATTATTGAAGTATCTCAATTTCAATCAAAATTGACTGATGTTAAATGTGCTAATGTTGTCTTGCTTAATTGCTTGCAACATTTGCATGTTGCTTCTAACTCTAAGTTGTGGCAGTATTGTAGCACTTTGCACAATGAAATACTTGCCACTTCTGATCTGGGTGTTGCTTTTGAAAAGCTTGCTCAGTTGTTAATTGTTTTGTTTGCTAATCCAGCTGCTGTGGATAGCAAGTGCCTGACTAGTATTGAAGAAGTTTGCGACGATTACGCAAAGGACAATACTGTTTTGCAGGCTTTACAGAGTGAATTTGTTAATATGGCTAGCTTCGTTGAATATGAAGTTGCTAAGAAAAATCTTGATGAGGCGCGTTCTAGTGGTTCTGCTAATCAACAGCAGTTAAAACAGCTAGAGAAAGCCTGTAATATTGCTAAATCTGCTTATGAACGCGACCGTGCTGTAGCAAGAAAGTTGGAGCGTATGGCAGATTTGGCTCTCACTAATATGTATAAAGAAGCTAGAATTAATGATAAGAAGAGTAAGGTTGTTTCTGCCTTGCAAACTATGCTTTTTAGTATGGTGCGTAAGTTAGATAATCAAGCTCTGAATTCAATATTAGATAATGCTGTGAAGGGTTGTGTACCATTGAATGCAATCCCTTCATTGGCTGCAAATACTCTGACTATAATTGTACCAGATAAAAGTGTTTACGATCAGGTAGTTGACAATGTCTATGTTACCTATGCGGGTAATGTATGGCAGATTCAAACTATCCAAGATTCAGATGGTACAAATAAGCAGTTGAATGAGATATCTGATGATTGTAACTGGCCACTAGTTATTATTGCAAATCGGCATAATGAGGTATCTGCTACCGTTTTGCAAAATAATGAATTAATGCCTGCTAAGTTGAAAACTCAGGTTGTTAATAGTGGTCCAGATCAGACTTGTAACACACCTACTCAATGTTACTATAATAATAGTAACAATGGGAAGATTGTTTATGCTATACTTAGTGATGTTGATGGTCTTAAGTATACAAAGATTCTTAAAGATGATGGCAATTTTGTTGTTTTGGAGTTAGATCCTCCTTGTAAATTTACTGTTCAAGATGTTAAAGGTCTTAAAATTAAGTACCTTTATTTTGTAAAAGGTTGTAACACACTAGCAAGAGGCTGGGTTGTTGGTACAATTTCTTCTACAGTTAGATTGCAAGCTGGAACTGCTACTGAGTATGCTTCCAACTCATCTATATTATCTTTATGTGCGTTTTCTGTAGATCCTAAGAAAACGTATTTAGATTTTATACAGCAGGGAGGAACACCTATTGCCAATTGTGTTAAAATGTTGTGTGACCATGCTGGTACCGGTATGGCCATTACTGTTAAACCCGATGCTACCACTAATCAGGATTCATATGGTGGTGCGTCTGTTTGTATATATTGCCGCGCACGAGTTGAACACCCAGATGTTGATGGGTTGTGCAAATTACGCGGCAAGTTTGTACAAGTGCCTGTAGGTATAAAAGATCCTGTGTCTTATGTTTTGACACATGATGTTTGTCAAGTTTGTGGATTTTGGCGGGATGGAAGTTGTTCATGTGTTAGCACTGACACTACTGTTCAGTCAAAAGATACTAATTTTTTAAACGGGTTCGGGGTACGAGTGTAG
Protein
MSKINKYGLELHWAPEFPWMFEDAEEKLDNPSSSEVDIVCSTTAQKLETGGICPENHVMVDCRRLLKQECCVQSSLIREIVMNTRPYDLEVLLQDALQSREAVLVTPPLGMSLEACYVRGCNPNGWTMGLFRRRSVCNTGRCAVNKHVAYQLYMIDPAGVCFGAGQFVGWVIPLAFMPVQSRKFIVPWVMYLRKCGEKGAYNKDHKRGGFEHVYNFKVEDAYDLVHDEPKGKFSKKAYALIRGYRGVKPLLYVDQYGCDYTGGLADGLEAYADKTLQEMKALFPVWSQELPFDVTVAWHVVRDPRYVMRLQSASTIRSVAYVANPTEDLCDGSVVIKEPVHVYADDSIILRQHNLVDIMSCFYMEADAVVNAFYGVDLKDCGFVMQFGYIDCEQDLCDFKGWVPGNMIDGFACTTCGHVYETGDLLAQSSGVLPVNPVLHTKSAAGYGGFGCKDSFTLYGQTVVYFGGCVYWSPARNIWIPILKSSVKSYDGLVYTGVVGCKAIVKETNLICKALYLDYVQHKCGNLHQRELLGVSDVWHKQLLLNRGVYKPLLENIDYFNMRRAKFSLETFTVCADGFMPFLLDDLVPRAYYLAVSGQAFCDYADKICHAVVSKSKELLDVSLDSLSAAIHYLNSKIVDLAQHFSDFGTSFVSKIVHFFKTFTTSTALAFAWVLFHVLHGAYIVVESDIYFVKNIPRYASAVAQAFRSVAKVVLDSLRVTFIDGLSCFKIGRRRICLSGSKIYEVERGLLHSSQLPLDVYDLTMPSQVQKAKQKPIYLKGSGSDFSLADSVVEVVTTSLTPCGYSEPPKVADKICIVDNVYMAKAGDKYYPVVVDGQVGLLDQAWRVPCAGRRVTFKEQPTVNEIASTPKTIKVFYELDKDFNTILNTACGVFEVDDTVDMEEFYAVVVDAIEEKLSPCKELEGVGAKVSAFLQKLEDNSLFLFDEAGEEVLAPKLYCAFTAPEDDDFLEESGVEEDDVEGEETDLTVTSAGEPCVASEQEESSEILEDTLDDGPCVETSDSQVEEDVEMSDFVDLESVIQDYENVCFEFYTTEPEFVKVLDLYVPKATRNNCWLRSVLAVMQKLPCQFKDKNLQDLWVLYKQQYSQLFVDTLVNKIPANIVVPQGGYVADFAYWFLTLCDWQCVAYWKCIKCDLALKLKGLDAMFFYGDVVSHVCKCGESMVLIDVDVPFTAHFALKDKLFCAFITKRSVYKAACVVDVNDSHSMAVVDGKQIDDHRVTSITSDKFDFIIGHGMSFSMTTFEIAQLYGSCITPNVCFVKGDIIKVSKRVKAEVVVNPANGHMAHGGGVAKAIAVAAGQQFVKETTDMVKSKGVCATGDCYVSTGGKLCKTVLNVVGPDARTQGKQSYALLERVYKHLNKYDCVVTTLISAGIFSVPSDVSLTYLLGTAEKQVVLVSNNQEDFDLISKCQITAVEGTKKLAERLSFNVGRSIVYETDANKLILSNDVAFVSTFNVLQDVLSLRHDIALDDDARTFVQSNVDVVPEGWRVVNKFYQINGVRTVKYFECPGGIDICSQDKVFGYVQQGSFNKATVAQIKALFLDKVDILLTVDGVNFTNRFVPVGESFGKSLGNVFCDGVNVTKHKCDINYKGKVFFQFDNLSSEDLKAVRSSFNFDQKELLAYYNMLVNCSKWQVVFNGKYFTFKQANNNCFVNVSCLMLQSLNLKFKIVQWQEAWLEFRSGRPARFVSLVLAKGGFKFGDPADSRDFLRVVFSQVDLTGAICDFEIACKCGVKQEQRTGVDAVMHFGTLSREDLEIGYTVDCSCGKKLIHCVRFDVPFLICSNTPASVKLPKGVGSANIFKGDKVGHYVHVKCEQSYQLYDASNVKKVTDVTGNLSDCLYLKNLKQTFKSVLTTYYLDDVKKIEYNPDLSQYYCDGGKYYTQRIIKAQFKTFEKVDGVYTNFKLIGHTICDILNAKLGFDSSKEFVEYKVTEWPTATGDVVLATDDLYVKRYERGCITFGKPVIWLSHEQASLNSLTYFNRPLLVDENKFDVLKVDDVDDGGDISESDAKESKEINIIKLSGVKKPFKVEDSVIVNDDTSEIKYVKSLSIVDVYDMWLTGCRYVVRTANALSMAVNVPTIRKFIKFGMTIVSIPIDLLNLREIKPVFNVVKAVRNKISACFNFIKWLFVLLFGWIKISADNKVIYTTEVASKLTCKLVALAFKNAFLTFKWSVVARGACIIATIFLLWFNFIYANVIFSDFYLPKIGFLPTFVGKIAQWIKSTFSLVTICDLYSIQDVGFKNQYCNGSIACQFCLAGFDMLDNYKAIDVVQYEADRRAFVDYTGVLKIVIELIVSYALYTAWFYPLFALISIQILTTWLPELFMLSTLHWSVRLLVSLANMLPAHVFMRFYIIIASFIKLFSLFRHVAYGCSKPGCLFCYKRNRSLRVKCSTIVGGMIRYYDVMANGGTGFCSKHQWNCIDCDSYKPGNTFITVEAALDLSKELKRPIQPTDVAYHTVTDVKQVGCYMRLFYERDGQRTYDDVNASLFVDYSNLLHSKVKGVPNMHVVVVENDADKANFLNAAVFYAQSLFRPILMVDKNLITTANTGTSVTETMFDVYVDTFLSMFDVDKKSLNALIATAHSSIKQGTQICKVLDTFLSCARKSCSIDSDVDTKCLADSVMSAVSAGLELTDESCNNLVPTYLKGDNIVAADLGVLIQNSAKHVQGNVAKIAGVSCIWSVDAFNQLSSDFQHKLKKACCKTGLKLKLTYNKQMANVSVLTTPFSLKGGAVFSYFVYVCFLLSLVCFIGLWCLMPTYTVHKSDFQLPVYASYKVLDNGVIRDVSVEDVCFANKFEQFDQWYESTFGLSYYSNSMACPIVVAVVDQDLGSTVFNVPTKVLRYGYHVLHFITHALSADGVQCYTPHSQISYSNFYASGCVLSSACTMFAMADGSPQPYCYTEGLMQNASLYSSLVPHVRYNLANAKGFIRFPEVLREGLVRIVRTRSMSYCRVGLCEEADEGICFNFNGSWVLNNDYYRSLPGTFCGRDVFDLIYQLFKGLAQPVDFLALTASSIAGAILAVIVVLVFYYLIKLKRAFGDYTSIVFVNVIVWCVNFMMLFVFQVYPTLSCVYAICYFYATLYFPSEISVIMHLQWLVMYGTIMPLWFCLLYISVVVSNHAFWVFAYCRRLGTSVRSDGTFEEMALTTFMITKDSYCKLKNSLSDVAFNRYLSLYNKYRYYSGKMDTAAYREAACSQLAKAMDTFTNNNGSDVLYQPPTASVSTSFLQSGIVKMVNPTSKVEPCIVSVTYGNMTLNGLWLDDKVYCPRHVICSASDMTNPDYTNLLCRVTSSDFTVLFDRLSLTVMSYQMQGCMLVLTVTLQNSRTPKYTFGVVKPGETFTVLAAYNGKPQGAFHVTMRSSYTIKGSFLCGSCGSVGYVLMGDCVKFVYMHQLELSTGCHTGTDFNGDFYGPYKDAQVVQLPVQDYIQSVNFVAWLYAAILNNCNWFVQSDKCSVEDFNVWALSNGFSQVKSDLVIDALASMTGVSLETLLAAIKRLKNGFQGRQVMGSCSFEDELTPSDVYQQLAGIKLQSKRTRLVKGIVCWIMASTFLFSCIITAFVKWTMFMYVTTNMLSITFCALCVISLAMLLVKHKHLYLTMYIIPVLFTLLYNNYLVVYKQTFRGYVYAWLSYYVPSVEYTYTDEVIYGMLLLIGMVFVTLRSINHDLFSFIMFVGRVISVVSLWYMGSNLEEEILLMLASLFGTYTWTTALSMAAAKVIAKWVAVNVLYFTDIPQIKIVLVCYLFIGYIISCYWGLFSLMNSLFRMPLGVYNYKISVQELRYMNANGLRPPKNSFEALMLNFKLLGIGGVPIIEVSQFQSKLTDVKCANVVLLNCLQHLHVASNSKLWQYCSTLHNEILATSDLGVAFEKLAQLLIVLFANPAAVDSKCLTSIEEVCDDYAKDNTVLQALQSEFVNMASFVEYEVAKKNLDEARSSGSANQQQLKQLEKACNIAKSAYERDRAVARKLERMADLALTNMYKEARINDKKSKVVSALQTMLFSMVRKLDNQALNSILDNAVKGCVPLNAIPSLAANTLTIIVPDKSVYDQVVDNVYVTYAGNVWQIQTIQDSDGTNKQLNEISDDCNWPLVIIANRHNEVSATVLQNNELMPAKLKTQVVNSGPDQTCNTPTQCYYNNSNNGKIVYAILSDVDGLKYTKILKDDGNFVVLELDPPCKFTVQDVKGLKIKYLYFVKGCNTLARGWVVGTISSTVRLQAGTATEYASNSSILSLCAFSVDPKKTYLDFIQQGGTPIANCVKMLCDHAGTGMAITVKPDATTNQDSYGGASVCIYCRARVEHPDVDGLCKLRGKFVQVPVGIKDPVSYVLTHDVCQVCGFWRDGSCSCVSTDTTVQSKDTNFLNGFGVRV
Summary
Function
The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).
Catalytic Activity
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
Decay of host mRNAs by virus
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Host cytoplasm
Host gene expression shutoff by virus
Host membrane
Host mRNA suppression by virus
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host IRF3 by virus
Inhibition of host ISG15 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Protease
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
Pubmed
EMBL
Proteomes
PRIDE
Pfam
Interpro
IPR002705
Pept_C30/C16_B_coronavir
IPR014829 NSP8
IPR014827 Viral_protease
IPR002589 Macro_dom
IPR036499 NSP9_sf
IPR032505 Corona_NSP4_C
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR032592 NAR_dom
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR013016 Peptidase_C30/C16
IPR022570 B-CoV_NSP1
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR038083 R1a/1ab
IPR014829 NSP8
IPR014827 Viral_protease
IPR002589 Macro_dom
IPR036499 NSP9_sf
IPR032505 Corona_NSP4_C
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR032592 NAR_dom
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR013016 Peptidase_C30/C16
IPR022570 B-CoV_NSP1
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR038083 R1a/1ab
SUPFAM
ProteinModelPortal
PDB
6JIJ
E-value=1e-177 Score= 547 Identity=84.16%
Cov(Q)=6.91% Cov(P)=100.66%
3D Structure
Ontologies
GO
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0039502 P:suppression by virus of host type I interferon-mediated signaling pathway
GO:0008242 F:omega peptidase activity
GO:0039579 P:suppression by virus of host ISG15 activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0039595 P:induction by virus of catabolism of host mRNA
GO:0019079 P:viral genome replication
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0039502 P:suppression by virus of host type I interferon-mediated signaling pathway
GO:0008242 F:omega peptidase activity
GO:0039579 P:suppression by virus of host ISG15 activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0039595 P:induction by virus of catabolism of host mRNA
GO:0019079 P:viral genome replication
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Topology
Length:
4383
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
399.471559999999
Exp number, first 60 AAs:
0
Total prob of N-in:
0.37913
outside
1 - 2137
TMhelix
2138 - 2156
inside
2157 - 2192
TMhelix
2193 - 2215
outside
2216 - 2308
TMhelix
2309 - 2331
inside
2332 - 2351
TMhelix
2352 - 2374
outside
2375 - 2751
TMhelix
2752 - 2774
inside
2775 - 3028
TMhelix
3029 - 3051
outside
3052 - 3060
TMhelix
3061 - 3083
inside
3084 - 3089
TMhelix
3090 - 3112
outside
3113 - 3121
TMhelix
3122 - 3144
inside
3145 - 3558
TMhelix
3559 - 3581
outside
3582 - 3585
TMhelix
3586 - 3608
inside
3609 - 3612
TMhelix
3613 - 3635
outside
3636 - 3654
TMhelix
3655 - 3677
inside
3678 - 3683
TMhelix
3684 - 3701
outside
3702 - 3710
TMhelix
3711 - 3733
inside
3734 - 3752
TMhelix
3753 - 3775
outside
3776 - 4383
