Strain
Canine_KC175341
(Region: USA: Ithaca, NY; Strain: Canine coronavirus strain S378, complete genome.; Date: 1978)
Gene
ORF1a polyprotein
Description
Annotated in NCBI,
ORF1a polyprotein
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGAGTTCCAAACAATTCAAGATCCTCGTCAACGAGGACTACCAAGTCAACGTGCCTAGTCTTCCTATTCGTGACGTGTTACAGGAAATTAAGTACTGTTATCGTAATGGATTTGAGGGCTATGTCTTCGTGCCAGAATACTGTCGTGACCTAGTTGATTGCGATCGTAAGGATCACTACGTCATTGGTGTTCTTGGTAACGGAATAAGTGATCTCAAACCTGTTCTTCTTACCGAACCCTCCGTCATGCTGCAAGGTTTTATTGTTAAAGCTATCTGCAATGGCGCTCTTGAGGACTTTGACCTTAAAATTGCTCGTACTGGAAGAGGTGCTATATATGTAGACCAATACATGTGTGGTGCTGATGGAAAACCAGTCATTGAAGGCGATTTTAAGGACTACTTCGGTGATGAGGACATTATTGAATTTGAAGGAGAAGAGTACCATTGCGCTTGGACAACAGTGCGCGATGAAAAACTGCTTAATCAGCAAACTCTCTTTACTATTCAGGAAATACAATATAATCTGGACATTCCTCACAAACTGCCAAATTGTGCTACTAGACATGTAGCACCACCAGTCAAAAAGAACTCTAAAATAGTTCTGTCTGAAGATTACAAGAAGCTTTATGAAATCTTTGGATCACCCTTTATGGGAAATGGTGACTGTCTGAGCAAATGCTTTGACACTCTTCATTTCATTGCTGCAACTCTTAGATGTCCATGTGGCGCAGAGAGTAGTGGTGTTGGTGATTGGACTGGTTTTAAGACTGCTTGTTGTGGTCTTGTTGGCAAAGTTTTGGGTGTCACGCTGGGTGATATTAAGCCTGGTGATGCTGTTGTCACTAGTATGAGCGCTGGTAAAGGAGTTAAATTCTTTGCCAATTGTGTTCTTCAGCATGCTGGTGATGTTGAAAATGTCTCTGTTTGGAAAGTGATTAAAACTTTTACTGTTGACGAGACTGTTTGCACCCCTGACTTTGAAGGCGAATTGAACGACTTCATTAAACCTGAGAGTAAATCACCAACTGCATGCAGCATTAAAAGAGCATTCATTACTGGTGAAATTGATGATGCTGTACATGATTGTATCATTACAGGAAAATTGGATCTTAGCACTAATCTTTTTGGTAATGTTGGTCTACTATTCAAGAAAACTCCCTGGTTTGTACAAAAGTGTGGTGCACTTTTTGTTGATGCTTGGAAAGTAGTAGAGGAACTTCTTGGTTCACTCAAACTCACATACAAGCAAATTTACGAAGTTGTAGCATCACTTTGCACTTCTGCTTTTACGATTGTAAACTACAAACCAACGTTTGTAGTTTTAGACAATCGCGTTAAAGATCTTGTAGACAAGTGTGTGAATATTCTTGTAAAGGCTTTTGATGTTTTTACGCAGACTATCACAATTGCTGGTATTGAAGCCAAATGCTTTGTGCTTGGTGCTAAATACCTGTTGTTCAATAATGCACTTGTCAAACTTGTCAGTGTTAAAATACTTGGCAAGAAGCAAAAGGGTCTCGAAAGTGCATTCTTTGCTACTAGCTTGGTTGGTGCAACTGTTAATGTGACACCGAAAAGAACTGAGACTGCCACTATTAGCTTGAACAAAGTTGATGATGTGGTGGCACCAGGAGAGGGTTATATTGTCATTGTTGGTGATATGGCTTTCTACAAGAGTGGTGAATACTACTTCATGATGGCTAGTCCTGATTCTGTTCTTATTAACAATGTTTTTAAAGCAGCTAGAGTCCCATCTTATGACATCGTGTATGATGTTGATGATGACACCAAGGGCAAGATGACTGCAAAACTTGGTTCATCATTTGAATATGATGGTGATATTGATGCTGCTGTTACTAAAGTCAATGAGTTACTCATTGAATTTAAACAACAAAGTCTTTGTTTTAGAGCACTTAAAGATGACAAAGTCATTTTTGTTGAAGCTTATCTTAAAAAGTACAAAATGCCTGCATGCCTTGCAAAACACATTGGTTTGTGGAACATCATAAAGAAAGATTCATGTAAGAAGGGTTTTCTTAGTCTCTTCGATCATTTGAATGAATTGGAAGATATCAAAGAAACTAATGTTCAGGCTATTAAGAATATTCTTTGCCCTGAGCTTCTTCTTGATCTGGATTATGGTGCCATTTGGTACAAGTGTATGCCGAGTTGCTCTGATCCATCAGTGTTGGGGAGTGTCCAACTTTTGATTGGAAATGGTGTGAAAGTGGTTTGTGATGGCTGCAAAGTTTTTGCTAACCAACTTTCAAATGGTTACAATAGGCTCTGTAATGCGGCTCGCACTGACATTGAGATCGGTGGTATACCATTTTCTACTTTCAAAACACCTACAAACACTTTCATTGAAATGACAGATGCTATCTACTCAGTTATTGAGCAAAACAAGGCTCTATCCTTTGGAAACGCTGATGTGCCTGTTGTAGATAATGGTATCATTTCTACTGATGATTGGTCTGAACCCATTTTGCTCGAACCTGCTGAATATGTGGAACCCAAGAACAATGGTAATATCATTGTTATTGCTGGTTACACGTTTTATAAAGATGAGGATGAACATTTCTATCCTTATGGTTCTGGTAAAATTGTGCAAAGAATGTACAATAAAATGGGTGGTGGTGACAAAACTGTGTCATTTGCAGAAGAAGTAGATGTTCAAGAAATTGCACCTGTTACACGTGTCAAGCTTGAGTTTGAGTTTGACAACGAAATTGTAACTAGTGTTCTTGAGCAAGCTATTGGTACCAAATATAAATTTACTGGTACAACTTGGGAAGAATTTGAGGAGTCTATTTCTGAAGAACTTGATGCAATTTTTGATACTCTAGCAAACCAAGGTGTCGAACTTGAAGGCTATTTCATTTATGATACCTGTGGTGGCTTTGACATCAAAAACCCAGATGGTATTATGATCTCTCAGTATGACATCAGTAATACCGCTGATGAGTCTGACTCTGGTGCATCAAGTGAAGGAGATGAAGTTGAATCTGTTGAAGAAGACTCCAAAACTGAAATCGTAGAAGCATCTGAAGCTATTGATGAGACATCTTCTCAAGAAGAGGTAGAAACTGTAGAGGTTGAAGATATTACTTCTGCAGAAGATGTTGAAATTGTGGAAGTGTCTGCTAAGGATGACCCATGGGCTGCTGCTGTTGATGTACAAGAAGCTGAACATGTTAAGCCTTCTTTACCACCTTTCAAGACAACAAATCTTAATGGTAAAATCATCCTTAAACAACAGGATAACAATTGTTGGATAAATGCTTGTTGCTATCAGCTGCAGGCTTTTGACTTTTTCAACAATGAAGCTTGGGAAAAATTTAAGAAAGGTGATGTTATGGATTTTGTCAATCTCTGTTATGCAGCCACAACACTTGAAAGGGGTCATTCTGGTGATGCAGAGTATCTTCTTGAACTTATGCTCAATGACTATAGTACGGCAAAAATAGTACTTGTTGCCAAGTGTGGCTGTGGCGAAAAAGAAATTGTATTGGAAAGAACTGTGTTTAAACTCACTCCATTAAAGGATGAGTTTAATTATGGTGTTTGTGGTGAATGTATGCAAATTAACAGGTGCAAAGTTGCAAGTGTTGAAGGATCTGGTTTGTTTGTACATGATTGCATTGACAAACAGACTCCCACGTCACAATTTATTGTTAAGCCAATTATGCATGCAGTGTACACTGGAACAACTCAAAATGGTCATTACATGGTCGATGACATTGAACATGATTATTGTGTTGATGGTATGGGTATTAAGCCACTTAAGAAACGACACTACACATCTACACTATTCATCAATGCTAATGTAATGACTAGAGTTGAAAAACCAAAACAAGAACCTAAAGTTGAAAAAGTTGAACAGCAACCAGTGGAAGAAAAGAAACTCTCAAGTGAAAAAGAGGAAGTTCAAAGTCCTAAAAACGATGATCTTATACTTCCATTTTACAAAGCTGGTAAACTTTCCTTTTATCAGGGTGATTTGGATGTTTTGATCAATTTCTTGGAACCTGATGTCATTGTTAATGCTGCCAATGGTGATCTTAAACACATGGGTGGTGTCGCAAGAGCCATCGATGTTTTCACTAGTGGCAAATTAACAGAACGTTCTAAGGAGTATCTTAAAAAGAACAAATCTATTGCTCCTGGTAATGCTGTTTTCTTTGAAAATGTCATTGAGAATCTTAGTGTTTTGAATGCTGTAGGACCACGTAATGGTGACAGCCGGATTGAAGGCAAACTTAATAATGTCTATAAGGCCATTGCAAAGTGTGAAGGAAAAATACTAACACCACTTATCAGTGTTGGTATTTTCAGTGTTAAACTTGAAATGTCATTGCAGTGCTTACTTAAGACTGTAAATGATAGAGAATTGAATGTCTTCGTGTACACTGATCAGGAGAGACAAGCCATTGAGAACTTCTTCTCTGGTTCTATCCCTGTCAAAGTTACTGAAGATAATGTCAACCATGAACGTGTGTCCGTTTCTTTTGACAAAACATACGGTGAACAACTTAAAGGTACCGTGGTCATTAAAGACAAAGATGTTACAAACCAGTTGCCTAGTGCTTTTGATGTTGGTCAGAAAGTTGTTAAGGCTATTGACGTAGATTGGCAAGCTCATTATGGTTTCCATGATGCTGCTGCCTTTAGTGCTAGTAGCCATGATGCTTACAAGTTTGAAGTCGTTACACATAGCAACTTCATTGTACATAAGCAGACTGATAACAACTGCTGGATTAATGCAATTTGTCTTGCATTACAGAGACTTAAACCTCAATGGAAATTTCCTGGTGTTAGAGGTCTTTGGAATGAATTTCTTGAGCGTAAAACACAAGGTTTTGTACACATGCTGTATCACATTTCAGGAGTAAAGAAAGGTGAGCCAGGTGATGCTGAATTGACTCTGCATAAACTTGGTGATTTGATGGATAATGATTGTGAAATCATTGTCACACATACTACAGCATGTGACAAATGCTCCAAAGTTGAAAAGTTTACTGGACCAGTGGTAGCAGCGCCGCTTGCAATTCATGGCACTGATGAAACATGTGTTCATGGTGTTAGTGTTAATGTTAAAGTTACTCAGATTAAGGGCACTGTTGCTATCACATCTTTGATTGGTCCTATTATTGGAGAAGTACTTGAAGCAACTGGTTACATCTGTTATAGAGGTTCAAGCAAGAATGGTCATTACACCTATTACGACAATCGTAATGGGTTAATGATTGATGCAGAAAAGGCTTACCACTTTAATAAAGACCTATTACAAGTCACGACAGCCATTGCAAGTAATTTTGTTGTCAAGAAACCACAAGTAGAAGAAAAACCTAAGACTCGTGTTTTTAACAGAGTAGATGAGCCTCCAAAGACTATAGTACAAGAACAAAAGTTGTTGGCTATTGAAAGTGGTGCTGATTACGCTCTCACAGAGTTTGGAAGATATGCTGACATGTTCTTTATGGCTGGAGACAAAATTCTTAGGTTTCTGCTTGAAGTCTTTAAATATCTGTTGGTTTTGTTTATGTGTCTTAGAAGTACTAAGATGCCTAAAGTTAAAGTAAAGCCACCTCATGCATTTAAAGACTTAGGTGCTAAAGTTAGAACACTTAATTACATGAGGCAATTGAACAAACCTGCTGTTTGGCGTTATGCAAAACTAGTTTTATTGCTAATAGCAATATATAATTTCTTTTATTTGTTTGTCAGTATACCAGTAGTTCATAAATTAACATGTAGTGGTGCTGTACAGGCATATAAAAATTCTAGTTTTGTGAAGTCAGAAGTTTGTGGTAACTCTATTCTTTGTAAAGCTTGTTTGGCTTCTTATGATGAGCTGGCTGACTTCCAACATCTTCAAGTTGCTTGGGATTTCAAGTCAGATCCACTCTGGAATAAACTGGTACAATTGTCTTATTTTGTATTTTTGGCTGTCTTTGGTAACAATTATGTTAGATGCTTTCTTATGTACTTTGTGTCCCAGTACCTCAACCTGTGGCTTTCTCACTTTGGTTACGTAGAGCACAGCTGGTTTTTGCATGTTGTCAGCTTTGAATCTATTTCAGCTGAGTTTGTGATTATAGTTATTTTGGTTAAGGCAGTTCTCGCTCTTAAACATATTGTCTTCTCATGCTCAAACCCATCTTGTAAAACTTGCTCTAAAACTGCAAGGCAGACACGTATTCCGATACAGGTTGTTGTTAATGGCTCTATGAAGACAGTTTATGTTCATGCCAACGGTACTGGTAAACTCTGCAAGAAACATAACTTTTATTGTAAGAATTGTGATTCTTATGGTTTTGAGAATACATTCATCTGTGACGAAATTGTTCGTGATCTCAGTAATAGTGTTAAACAGACTGTTTATGCTACTGATAGATCTCATCAAGAAGTCACAAAAGTTGAATGTTCAGATGGATTTTACAGGTTCTATGTTGGTGATGAATTCACTTCATATGATTATGATGTAAAACATAAGAAGTACAGTAGTCAAGAGGTTCTTAAGAACATGCTCTTGCTTGATGACTTTATTGTGTACAGTCCATCTGGTTCTTCTCTTGCAAACGTAAGAAATGCCTGTGTGTACTTTTCACAACTTATTGGTAAGCCTATTAAGATTGTTAACAGTGATTTACTTGAAGACCTCTCTGTAGACTTTAAAGGAGCACTTTTCAATGCTAAGAAGAATGTAATTAAGAATTCTTTCAATGTAGACGTTTCGGAATGTAAAAATCTTGATGAGTGCTATAAAGCTTGCAATCTTGATGTGTCATTTTCAACATTTGAAATGGCTGTCAATAATGCTCATAGATTTGGTATTTTGATTACTGACCGTTCTTTTAACAATTTTTGGCCATCAAAAGTCAAACCTGGTTCATCTGGTGTTTCAGCCATGGACATTGGTAAGTGTATGACCTCTGATGCTAAAATTGTTAATGCTAAAGTTTTGACCCAACGTGGTAAAAGTGTAGTTTGGCTTAGTCAAGATTTTGCTGCACTTAGCTCAACTGCGCAGAAAGTGTTGGTTAAAACTTTTGTGGAAGAAGGTGTCAACTTCTCACTCACATTTAATGCTGTAGGTTCAGATGATGATCTTCCTTATGAAAGATTCACTGAATCTGTGTCTCCAAAAAGTGGTTCAGGCTTTTTCGATGTACTCAAACAGCTTAAACAAATTGTGATGTTAGTTCTTGGTTTCATCTTTATCTATGGTTTGTGCTCTGTCTACAGTGTTGCTACACAGTCCTACATTGAATCTGCTGAAGGTTATGACTACATGGTTATTAAGAATGGAATTGTTCAACCTTTTGACGACACCATTTCATGTGTTCATAACACTTACAAAGGATTTGGTGAATGGTTTAAAGCTAAGTACGGTTTTATCCCTACTTTTGCCAAAGCATGCCCAATTGTTGTAGGAACTGTTTTTGATCTTGAAAACATGAGACCAATTCCTGATGTGCCTGCATATGTAGCTCTTGTTGGTAGATCTCTTGTTTTTGCTATTAATGCTGCTTTTGGTGTTACTAATATGTGCTATGATCATACTGGCAATGCAGTGAGTAAGGATTCTTACTTCGACACTTGTGTGTTTAATGCTGCATGCACCACTCTTACAGGTCTTGGTGGTACAATTGTTTATTGTGCAAAACAAGGTCTAGTTGAAGGTGCTAAGCTCTATAGTGATCTTATGCCTGACTATTATTATGAGCACGCTAGTGGTAACATGGTTAAATTGCCAGCAATTATTAGAGGACTTGGTCTACGGTTTGTAAAAACACAGGCTACGACTTACTGTAGAGTAGGAGAGTGTATTGACAGCAAAGCTGGTTTTTGCTTTGGTGGTGATAACTGGTTTGTCTATGACAAAGAGTTTGGTAATGGGTACATTTGTGGTAATTCTGTTTTAGGATTCCTTAAGAATGTCTTCAGACTCTTTAACTCTAACATGTCTGTGGTAGCCACATCAGGTGCGATGCTTGTTAACATTATCATTGCTTGCCTGGCTATTGCAATGTGTTATGGTGTTCTTAAATTTAAGAAAATATTTGGTGACTGCACTCTTCTGATTGTTATGATTATTGTTACACTTGTTGTAAACAATGTGTCTTATTTTGTCACTCAAAACACATTCTTTATGATTATTTATGCCATTGTTTATTACTTTACAACTAGGAAGCTTGCATACCCAGGCGTCCTTGATGCTGGGTTTATTATTGCTTACATTAACATGGCTCCATGGTATGTGATTACTGCATACATCTTAGTGTTCCTCTATGACTCACTTCCTTCACTGTTCAAACTTAAGGTTTCAACAAACCTTTTTGAAGGTGACAAATTTGTGGGTAACTTTGAATCTGCCGCTATGGGTACTTTTGTTATTGATATGCGTTCATATGAAACTATTGTTAATTCTACTCCTATTGATAGAATTAAATCATATGCTAACAGCTTCAATAAATATAAGTACTACACAGGTTCAATGGGAGAGGCTGACTACAGAATGGCTTGCTATGCTCATCTTGGTAAAGCTCTTATGGACTATTCTGTTAACAGAACAGACATGCTTTACACACCTCCTACTGTTAGTGTTAATTCCACACTTCAGTCCGGGTTGAGGAAAATGGCACAGCCTAGTGGTCTTGTAGAGCCTTGCATTGTAAGAGTTTCCTATGGTAACAATGTGCTTAATGGTTTATGGTTAGGAGATGAGGTCTTTTGTCCTAGACATGTCATTGCTAGTGATATCACACGTGTTATCAACTATGAAAATGAAATGTCCAGTGTGAGACTTCATAATTTTTCAGTTTCTAAAAATAATGTGTTTTTGGGTGTTGTGTCTGCCAAATATAAGGGTGTGAATCTCGTACTAAAAGTCAACCAGGTTAATCCTAACACACCAGAACATAAATTTAAGTCTATTAAAGCTGGTGAAAGTTTTAACATTCTTGCTTGTTATGAGGGATGTCCTGGTAGTGTTTATGGTGTTAACATGAGAAGCCAAGGTACCATTAAAGGTTCTTTTATTGCTGGTACTTGTGGATCAGTAGGTTATGTTTTGGAAAATGGAATTCTCTATTTTGTATACATGCATCACCTAGAACTTGGAAATGGCTCACATGTTGGTTCCAATTTTGAAGGAGAAATGTATGGTGGTTATGAAGATCAACCTAGCATGCAACTTGAAGGTACTAATGTCATGTCATCAGATAATGTGGTTGCATTTTTGTATGCTGCACTTATTAATGGTGAAAGGTGGTTTGTTACAAACACATCAATGTCATTAGAATCATACAATACATGGGCCAAAACTAACAGTTTCACAGAACTCTCTTCAACTGATGCATTTAGCATGTTGGCTGCGAAAACTGGTCAAAGTGTTGAGAAATTGCTAGATTGCATTGTAAGACTCAACAAAGGTTTTGGAGGTCGTACCATACTTTCTTATGGCTCACTGTGTGATGAGTTCACCCCAACTGAAGTCATAAGGCAAATGTATGGTGTAAATCTTCAAGCTGGTAAAGTAAAATCTTTCTTTTACCCTATTGTGACTGCAATGACAATTCTCTTTGCTTTTTGGCTTGAATTTTTCATGTACACACCCTTTACTTGGATTAACCCAGCTTTTGTTAGCTTTGTATTGGCTGTTACAACTTTGGTTTCGACTGTTTTTGTTTCAGGAATTAAACATAAGATGTTGTTCTTTATGTCTTTTGTTCTGCCTAGCGTTGTTCTTGTAACAGCACACAACTTATTCTGGGACTTTTCTTACTATGAAAGTCTTCAGTCAATTGTTGAAAATACTAACACTATGTTCTTGCCTGTTGACATGCAAGGTGTCATGCTCACAGTGTTTTGTTTTATTGTCTTTGTCACATACAGTGTTAGATTCTTCACTTGTAAACAATCATGGTTCTCACTCGCCGTGACAACTGTTCTTGTGATTTTTAACATGTTTAAAATTTTTGGAACATCTGATGAACCATGGACTGAAAACCAAATTGCTTTCTGCTTTGTGAACATGCTTACTATGATCGTCAGTCTTACTACAAAAGATTGGGTTGTTGTTGTTGCATCATACAGAGTCGCTTACTATATTGTTGTATGTGTAATGCCAGCTGCTTTTGTGTCTGACTTTGGCTTTATGAAGTGTATTAGCATTGTTTACATGGCTTGTGGTTATTTGTTCTGCTGTTATTATGGTATCCTCTATTGGGTTAACAGATTTACATGCATGACTTGTGGTGTTTATCAATTCACTGTGTCTGCAGCTGAACTTAAATACATGACTGCTAACAACCTTTCTGCACCTAAGAATGCGTATGACGCTATGATTCTTAGTGCTAAATTGATTGGTGTTGGAGGTAAGAGAAACATTAAAATTTCAACTGTACAGTCAAAACTTACAGAGATGAAATGTACAAATGTAGTCTTGCTCGGTCTTTTGTCTAAAATGCATGTTGAGTCTAACTCAAAAGAGTGGAACTATTGTGTAGGACTACACAATGAGATCAACCTTTGTGACGATCCTGAGATTGTTCTTGAAAAGTTGTTAGCTCTCATTGCATTCTTTTTGTCCAAACACAATACTTGTGACCTTAGCGAACTTATTGAATCATACTTTGAGAACACTACCATACTTCAGAGTGTGGCTTCAGCTTATGCTGCACTGCCTAGCTGGATTGCATTTGAAAAAGCTCGCGCTGATCTTGAAGAGGCTAAGAAAAATGATGTTAGCCCTCAAATTTTGAAACAGCTTACCAAGGCATTTAATATTGCCAAAAGTGATTTTGAGCGCGAAGCTTCAGTTCAAAAGAAGCTTGACAAAATGGCTGAGCAGGCTGCTGCTAGTATGTACAAAGAAGCACGAGCTGTGGATAGAAAATCAAAAGTCGTTTCTGCTATGCATAGCCTACTTTTTGGTATGCTTAAGAAACTCGATATGTCTAGTGTCAACACTATTATTGACCAGGCTCGTAATGGTGTTTTACCTTTAAGTATCATTCCAGCTGCATCAGCTACAAGACTTGTTGTTGTAACACCTAGTCTTGAAGTGTTTTCCAGGATTAGGCAAGAAAACAATGTTCATTATGCTGGTGCTATTTGGACTATCGTTGAAGTCAAAGACGCTAATGGTTCACATGTTCATCTTAAAGAAGTCACTGCTGCTAATGAATTAAATCTTACTTGGCCGTTGAGCATTACTTGTGAGAGAACCACAAAGCTTCAGAACAATGAAATTATGCCAGGTAAACTTAAAGAAAGAGCGGTCAAAGCTTCAGCCATTTTAGATGGTGAAGCTTTCGGCAGTGGAAAGGCTCTTATGGCAGCTGAAAGTGGCAAAAGCTTTATGTATGCATTTATAGCCTCAGACAACAATCTTAAGTATGTCAAATGGGAGAGCAATAATGATATCATACCTATTGAACTTGAAGCTCCATTGCGTTTCTATGTTGATGGCGTTAATGGTCCTGAAGTCAAGTATTTGTACTTCGTCAAGAACTTAAACACTCTTAGACGTGGTGCCGTTCTTGGTTATATCGGTGCAACAGTTCGTCTGCAAGCTGGTAAACCCACTGAACATCCATCTAATAGTAGTTTATTGACACTGTGTGCTTTCGCACCTGATCCAGCTAAAGCATACGTTGATGCTGTTAAAAGAGGTATGCAACCTGTTAACAACTGTGTGAAAATGCTCTCAAATGGTGCTGGTAATGGTATGGCTGTTACAAATGGCGTTGAAGCTAACACACAACAAGATTCTTATGGTGGCGCTTCTGTTTGCATTTACTGCAGATGCCATGTGGAACATCCCGCTATTGATGGAATGTGCCGTTACAAAGGCAAATTTGTACAAATACCAACTGGCACTCAAGACCCCATTCGCTTTTGTATTGAAAATGAAGTTTGTGTTGTCTGTGGCTGTTGGCTTAACAATGGTTGCATGTGCGATCGTACTTCTATGCAGAGTTCTACTGTTGATCAAAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGACTAG
Protein
MSSKQFKILVNEDYQVNVPSLPIRDVLQEIKYCYRNGFEGYVFVPEYCRDLVDCDRKDHYVIGVLGNGISDLKPVLLTEPSVMLQGFIVKAICNGALEDFDLKIARTGRGAIYVDQYMCGADGKPVIEGDFKDYFGDEDIIEFEGEEYHCAWTTVRDEKLLNQQTLFTIQEIQYNLDIPHKLPNCATRHVAPPVKKNSKIVLSEDYKKLYEIFGSPFMGNGDCLSKCFDTLHFIAATLRCPCGAESSGVGDWTGFKTACCGLVGKVLGVTLGDIKPGDAVVTSMSAGKGVKFFANCVLQHAGDVENVSVWKVIKTFTVDETVCTPDFEGELNDFIKPESKSPTACSIKRAFITGEIDDAVHDCIITGKLDLSTNLFGNVGLLFKKTPWFVQKCGALFVDAWKVVEELLGSLKLTYKQIYEVVASLCTSAFTIVNYKPTFVVLDNRVKDLVDKCVNILVKAFDVFTQTITIAGIEAKCFVLGAKYLLFNNALVKLVSVKILGKKQKGLESAFFATSLVGATVNVTPKRTETATISLNKVDDVVAPGEGYIVIVGDMAFYKSGEYYFMMASPDSVLINNVFKAARVPSYDIVYDVDDDTKGKMTAKLGSSFEYDGDIDAAVTKVNELLIEFKQQSLCFRALKDDKVIFVEAYLKKYKMPACLAKHIGLWNIIKKDSCKKGFLSLFDHLNELEDIKETNVQAIKNILCPELLLDLDYGAIWYKCMPSCSDPSVLGSVQLLIGNGVKVVCDGCKVFANQLSNGYNRLCNAARTDIEIGGIPFSTFKTPTNTFIEMTDAIYSVIEQNKALSFGNADVPVVDNGIISTDDWSEPILLEPAEYVEPKNNGNIIVIAGYTFYKDEDEHFYPYGSGKIVQRMYNKMGGGDKTVSFAEEVDVQEIAPVTRVKLEFEFDNEIVTSVLEQAIGTKYKFTGTTWEEFEESISEELDAIFDTLANQGVELEGYFIYDTCGGFDIKNPDGIMISQYDISNTADESDSGASSEGDEVESVEEDSKTEIVEASEAIDETSSQEEVETVEVEDITSAEDVEIVEVSAKDDPWAAAVDVQEAEHVKPSLPPFKTTNLNGKIILKQQDNNCWINACCYQLQAFDFFNNEAWEKFKKGDVMDFVNLCYAATTLERGHSGDAEYLLELMLNDYSTAKIVLVAKCGCGEKEIVLERTVFKLTPLKDEFNYGVCGECMQINRCKVASVEGSGLFVHDCIDKQTPTSQFIVKPIMHAVYTGTTQNGHYMVDDIEHDYCVDGMGIKPLKKRHYTSTLFINANVMTRVEKPKQEPKVEKVEQQPVEEKKLSSEKEEVQSPKNDDLILPFYKAGKLSFYQGDLDVLINFLEPDVIVNAANGDLKHMGGVARAIDVFTSGKLTERSKEYLKKNKSIAPGNAVFFENVIENLSVLNAVGPRNGDSRIEGKLNNVYKAIAKCEGKILTPLISVGIFSVKLEMSLQCLLKTVNDRELNVFVYTDQERQAIENFFSGSIPVKVTEDNVNHERVSVSFDKTYGEQLKGTVVIKDKDVTNQLPSAFDVGQKVVKAIDVDWQAHYGFHDAAAFSASSHDAYKFEVVTHSNFIVHKQTDNNCWINAICLALQRLKPQWKFPGVRGLWNEFLERKTQGFVHMLYHISGVKKGEPGDAELTLHKLGDLMDNDCEIIVTHTTACDKCSKVEKFTGPVVAAPLAIHGTDETCVHGVSVNVKVTQIKGTVAITSLIGPIIGEVLEATGYICYRGSSKNGHYTYYDNRNGLMIDAEKAYHFNKDLLQVTTAIASNFVVKKPQVEEKPKTRVFNRVDEPPKTIVQEQKLLAIESGADYALTEFGRYADMFFMAGDKILRFLLEVFKYLLVLFMCLRSTKMPKVKVKPPHAFKDLGAKVRTLNYMRQLNKPAVWRYAKLVLLLIAIYNFFYLFVSIPVVHKLTCSGAVQAYKNSSFVKSEVCGNSILCKACLASYDELADFQHLQVAWDFKSDPLWNKLVQLSYFVFLAVFGNNYVRCFLMYFVSQYLNLWLSHFGYVEHSWFLHVVSFESISAEFVIIVILVKAVLALKHIVFSCSNPSCKTCSKTARQTRIPIQVVVNGSMKTVYVHANGTGKLCKKHNFYCKNCDSYGFENTFICDEIVRDLSNSVKQTVYATDRSHQEVTKVECSDGFYRFYVGDEFTSYDYDVKHKKYSSQEVLKNMLLLDDFIVYSPSGSSLANVRNACVYFSQLIGKPIKIVNSDLLEDLSVDFKGALFNAKKNVIKNSFNVDVSECKNLDECYKACNLDVSFSTFEMAVNNAHRFGILITDRSFNNFWPSKVKPGSSGVSAMDIGKCMTSDAKIVNAKVLTQRGKSVVWLSQDFAALSSTAQKVLVKTFVEEGVNFSLTFNAVGSDDDLPYERFTESVSPKSGSGFFDVLKQLKQIVMLVLGFIFIYGLCSVYSVATQSYIESAEGYDYMVIKNGIVQPFDDTISCVHNTYKGFGEWFKAKYGFIPTFAKACPIVVGTVFDLENMRPIPDVPAYVALVGRSLVFAINAAFGVTNMCYDHTGNAVSKDSYFDTCVFNAACTTLTGLGGTIVYCAKQGLVEGAKLYSDLMPDYYYEHASGNMVKLPAIIRGLGLRFVKTQATTYCRVGECIDSKAGFCFGGDNWFVYDKEFGNGYICGNSVLGFLKNVFRLFNSNMSVVATSGAMLVNIIIACLAIAMCYGVLKFKKIFGDCTLLIVMIIVTLVVNNVSYFVTQNTFFMIIYAIVYYFTTRKLAYPGVLDAGFIIAYINMAPWYVITAYILVFLYDSLPSLFKLKVSTNLFEGDKFVGNFESAAMGTFVIDMRSYETIVNSTPIDRIKSYANSFNKYKYYTGSMGEADYRMACYAHLGKALMDYSVNRTDMLYTPPTVSVNSTLQSGLRKMAQPSGLVEPCIVRVSYGNNVLNGLWLGDEVFCPRHVIASDITRVINYENEMSSVRLHNFSVSKNNVFLGVVSAKYKGVNLVLKVNQVNPNTPEHKFKSIKAGESFNILACYEGCPGSVYGVNMRSQGTIKGSFIAGTCGSVGYVLENGILYFVYMHHLELGNGSHVGSNFEGEMYGGYEDQPSMQLEGTNVMSSDNVVAFLYAALINGERWFVTNTSMSLESYNTWAKTNSFTELSSTDAFSMLAAKTGQSVEKLLDCIVRLNKGFGGRTILSYGSLCDEFTPTEVIRQMYGVNLQAGKVKSFFYPIVTAMTILFAFWLEFFMYTPFTWINPAFVSFVLAVTTLVSTVFVSGIKHKMLFFMSFVLPSVVLVTAHNLFWDFSYYESLQSIVENTNTMFLPVDMQGVMLTVFCFIVFVTYSVRFFTCKQSWFSLAVTTVLVIFNMFKIFGTSDEPWTENQIAFCFVNMLTMIVSLTTKDWVVVVASYRVAYYIVVCVMPAAFVSDFGFMKCISIVYMACGYLFCCYYGILYWVNRFTCMTCGVYQFTVSAAELKYMTANNLSAPKNAYDAMILSAKLIGVGGKRNIKISTVQSKLTEMKCTNVVLLGLLSKMHVESNSKEWNYCVGLHNEINLCDDPEIVLEKLLALIAFFLSKHNTCDLSELIESYFENTTILQSVASAYAALPSWIAFEKARADLEEAKKNDVSPQILKQLTKAFNIAKSDFEREASVQKKLDKMAEQAAASMYKEARAVDRKSKVVSAMHSLLFGMLKKLDMSSVNTIIDQARNGVLPLSIIPAASATRLVVVTPSLEVFSRIRQENNVHYAGAIWTIVEVKDANGSHVHLKEVTAANELNLTWPLSITCERTTKLQNNEIMPGKLKERAVKASAILDGEAFGSGKALMAAESGKSFMYAFIASDNNLKYVKWESNNDIIPIELEAPLRFYVDGVNGPEVKYLYFVKNLNTLRRGAVLGYIGATVRLQAGKPTEHPSNSSLLTLCAFAPDPAKAYVDAVKRGMQPVNNCVKMLSNGAGNGMAVTNGVEANTQQDSYGGASVCIYCRCHVEHPAIDGMCRYKGKFVQIPTGTQDPIRFCIENEVCVVCGCWLNNGCMCDRTSMQSSTVDQSYLNECGVLVQLD
Summary
Function
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Catalytic Activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
Proteomes
Pfam
Interpro
IPR009003
Peptidase_S1_PA
IPR014822 NSP9
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR013016 Peptidase_C30/C16
IPR018995 RNA_synth_NSP10_coronavirus
IPR032505 Corona_NSP4_C
IPR032039 A-CoV_nsp1
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014829 NSP8
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR036499 NSP9_sf
IPR014822 NSP9
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR013016 Peptidase_C30/C16
IPR018995 RNA_synth_NSP10_coronavirus
IPR032505 Corona_NSP4_C
IPR032039 A-CoV_nsp1
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014829 NSP8
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR036499 NSP9_sf
SUPFAM
ProteinModelPortal
PDB
4F49
E-value=0.0 Score= 622 Identity=98.36%
Cov(Q)=7.57% Cov(P)=98.06%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0019079 P:viral genome replication
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0019079 P:viral genome replication
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 4 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 4 publications.
Topology
Length:
4015
Number of predicted TMHs:
16
Exp number of AAs in TMHs:
444.758849999999
Exp number, first 60 AAs:
0.0003
Total prob of N-in:
0.00045
outside
1 - 1886
TMhelix
1887 - 1909
inside
1910 - 1978
TMhelix
1979 - 2001
outside
2002 - 2015
TMhelix
2016 - 2038
inside
2039 - 2401
TMhelix
2402 - 2424
outside
2425 - 2493
TMhelix
2494 - 2516
inside
2517 - 2656
TMhelix
2657 - 2679
outside
2680 - 2688
TMhelix
2689 - 2711
inside
2712 - 2717
TMhelix
2718 - 2740
outside
2741 - 2743
TMhelix
2744 - 2766
inside
2767 - 3183
TMhelix
3184 - 3206
outside
3207 - 3210
TMhelix
3211 - 3233
inside
3234 - 3239
TMhelix
3240 - 3262
outside
3263 - 3281
TMhelix
3282 - 3304
inside
3305 - 3310
TMhelix
3311 - 3330
outside
3331 - 3361
TMhelix
3362 - 3384
inside
3385 - 3390
TMhelix
3391 - 3413
outside
3414 - 4015
