Strain
Human_229E_KF514433
(Region: USA: Nashville, TN; Strain: Human coronavirus 229E strain 229E/human/USA/933-40/1993, complete genome.; Date: 11-Mar-93)
Gene
replicase polyprotein 1a
Description
Annotated in NCBI,
replicase polyprotein 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGGCCTGCAACCGTGTGACACTTGCCGTAGCAAGTGATTCTGAAATTTCTGCATATGGCTGTTCTACTATTGCGCAAGCCGTCCGCCGTTATAGCGAGGCCGCTAGCAATGGTTTTAGGGCATGCCGATTTGTTTCATTAGATTTGCAGGATTGCATCGTTGGCATTGCAGATGATACATATGTTATGGGTCTGCATGGCAATCAGACGTTGTTTTGCAACATAATGAAATTTTCTGACCGTCCTTTTATGCTTCATGGGTGGTTGGTTTTTTCCAATTCAAATTACCTTTTGGAGGAATTTGATGTTGTCTTCGGTAAGAGAGGTGGTGGTAATGTGACATACACTGACCAGTATCTCTGTGGCGCCGATGGCAAACCTGTTATAAGTGAAGATTTATGGCAGTTTGTTGACCATTTCGGTGAGAACGAAGAAATTGTCATCAATGGTCGTACTTACGTTTGTGCTTGGCTTACTAAGCGTAAGCCCTTAGATTACAAACGTCAGAACAACCTCGCCATTGAAGAGATTGAATATGTGCATGGTGATGCTTTGCACACACTACGCAATGGTTCTGTTCTTGAAATGGCTAAGGAAGTGAAGACATCTAGTAAAGTTGTGTTAAGCGATGCTCTTGACAAACTTTACAAAGTCTTTGGTTCTCCTGTTATGACAAATGGTTCCAACATCCTAGAGGCCTTTACTAAACCTGTGTTTATTAGTGCATTAGTTCAATGTACTTGTGGTACCAAGTCTTGGTCTGTGGGTGATTGGACCGGTTTTAAATCCTCTTGTTGCAACTTGATCAGTAATAAACTGTGTGTTGTTCCCGGTAATGTTAAACCTGGTGATGCTGTGATTACTACTCAGCAAGCTGGTGCTGGTGTTAAGTATTTTTGTGGCATGACTCTTAAGTTTGTTGCAAATATTGAAGGTGTCTCTGTTTGGAGAGTGATTGCTCTTCAGAGTGTTGATTGCTTTGTTGCTTCTTCCACTTTTGTAGAAGAGGAACATGTTAATAGAATGGACACATTCTGCTTCAATGTACGCAATAGTGTTACTGATGAGTGTCGTCTGGCCATGTTGGGTGCTGAAATGACTAGTAATGTCAGAAGACAAGTTGCTTCAGGTGTCATAGACATTAGTACCGGCTGGTTTGATGTTTATGATGACATCTTTGCTGAAAGCAAACCATGGTTTGTTCGCAAGGCTGAAGACATTTTTGGCCCTTGTTGGTCCGCTCTTGTTTCTGCACTTAAACAACTTAAAGTCACTACAGGTGAACTTGTGAGATTTGTTAAGTCTATTTGCAATTCAGCTGTTGCTGTCGTGGGTGGTACTATACAAATTCTCGCTAGTGTGCCTGAGAAGTTTTTGAATGCGTTTGACGTGTTTGTCACAGCTATTCAAACTGTCTTTGACTGTGCTGTTGAAACTTGTACTATTGCCGGTAAAGCATTTGACAAGGTTTTTGACTATGTCTTGCTTGATAATGCGCTTGTAAAACTTGTCACCACAAAGCTTAAGGGTGTTCGTGAAGGTGGCCTTAATAAAGTTAAGTATGCAACAGTTGTTGTTGGTTCCACTGAAGAAGTTAAATCTTCACGTGTTGAACGTAGCAATGCTGTACTTACAATCGCCAACAATTATTCCAAACTTTTTGATGAAGGGTATACTGTTGTAATTGGCGATGTGGCGTACTTTGCTAGTGACGGCTACTTCCGTCTTATGGCCAGTCCAAATAGTGTGTTGACTACTGCAGTCTATAAACCATTGGTTGATTTTAATGTGAATGTTATGGGTACTAGACCTGAAAAATTTCCAACCACTGTGACTTGTGAAAATTTAGAGTCTGCTGTTTTGTTTGTTAATGACAAAATCATTGAATTCCAATTGGATTACAGTATTGATGTCATTGATAATGAAATAATTGTCAAACCTAATATCAGCTTATGTGTTCCACTTTATGTGAGAGACTATGTTGACAAATGGGATGATTTTTGCAGACAATATAGTAACGAGTCTTGGTTTGAGGATGATTACAAGGCTTTTATCAGTGTTTTGGATATCACTGATGCTGCTGTGAAAGCTGCAGAGTCTAAAGCCTTCGTTGATACTATTGTTCCACCTTGCCCATCTATTTTGAAAGTTATAGATGGAGGCAAAATATGGAATGGTGTTATTAAAAATGTTAACTATGTTAGAGACTGGCTTAAGTCTTTGAAGTTAAATCTCACACAACAGGGTTTGCTTGGAACATGTGCAAAGCGTTTTAAACGTTGGCTTGGCATTTTGCTAGAGGCCTATAATGCGTTTTTAGACACTGTTGTTTCTACTGTTAAAATTGGTGGTTTGACCTTTAAAACATATGCTTTTGATAAACCTTACATTGTGATACGTGATATCGTGTGTAAGGTTGAAAATAAAACAGAAGCAGAATGGATTGAACTTTTTCCACATAATGACAGGATTAAGTCTTTTAGTACCTTTGAGAGTGCTTACATGCCAATTGCAGACCCTACACATTTTGACATTGAAGAAGTTGAACTTTTAGATGCAGAGTTTGTAGAACCAGGCTGTGGTGGTATTTTGTCAGTAATAGATGAGCACGTCTTTTATAAGAAGGATGGTGTTTATTATCCATCAAATGGTACTAACATTCTACCTGTTGCATTTACAAAAGCCGCTGGTGGTAAAGTTTCATTTTCTGATGACGTTGAAGTAAAAGACATTGAACCTGTTTACAGAGTCAAGCTTTGCTTTGAGTTTGAAGATGAAAAACTTGTAGATGTTTGTGAAAAGGCAATTGGCAAGAAAATTAAACATGACGGTGACTGGGATAGCTTTTGTAAGACTATTCAATCAGCACTTTCTGTTGTTTCATGCTATGTAAATCTACCTACTTATTACATTTATGATGAAGAAGGCGGTAATGACTTGAGTTTGCCCGTTATGATTTCTGAATGGCCTCTTTCTGAGTCAGATAAGGAAGAAGATGTTCAACAAGCTCAACAAGAAGCTACTCATATTGCTGAGGATGTTGTTGACCAAGTTGAAGAAGTCAATAGCATTTTTGACATTGAGACAGTGGATGTTAAACATGAGATGAGTCCTTTTGAAATGCCATTTGAAGAGTTAAATGGTTTAAAGATACTTAAACAATTGGATAACAACTGCTGGGTTAACTCAGTTATGTTACAAATACAATTAACTGGTATACTTGATGGTGATTATGCTATGCAGTTTTTTAAAATGGGCCGAGTTGCCAAGATGATTGAACGCTGCTACACTGCTGAGCAATGTATACGTGGTGCTATGGGTGATGTTGGTTTGTGTATGTATAGACTTCTTAAAGACTTACACACTGGTTTTATGGTTATGGATTATAAATGTAGTTGTACCAGTGGTAGGCTTGAAGAATCGGGAGCTGTTTTGTTTTGTACGCCCACTAAGAAGGCGTTTCCTTATGGTACTTGTCTAAATTGTAACGCACCTCGCATGTGTACAATTAGGCAGTTACAAGGTACCATAATATTTGTGCAACAAAAACCAGAACCTGTTAACCCTGTTTCTTTTGTTGTTAAACCAGTCTGCTCATCAATTTTTCGTGGTGCTGTGTCTTGTGGTCATTACCAGACTAACATCTATTCACAAAATTTGTGTGTGGATGGTTTTGGTGTTAACAAGATTCAGCCCTGGACAAATGATGCACTTAATACTATTTGTATTAAGGATGCAGATTATAATGCAAAAGATGAAATATCTGTTACACCAATTAAAAACACAGTTGATACAATACCTAAGGAAAAATTTGTTGTTAAAGAGAAGTTGAACGCCTTCCTCGTTCATGACAATGTAGCTTTCTACCAAGGTGATGTTGATACTGTTGTTAATGGTGTTGACTTTGACTTTATTGTAAATGCTGCTAATGAGAACCTTGCTCATGGTGGAGGACTTGCCAAAGCTTTAGATGTGTACACTAAAGGTAAACTTCAACGTTTATCCAAAGAACACATTGGATTAGCGGGTAAAGTAAGAGTTGGTACAGGAGTTATGGTTGAGTGTGATAGCCTTAGAATTTTTAATGTTGTTGGTCCACGCAAGGGTAAACATGAACGTGATTTACTCGTAAAAGCTTACAACACTATTAATAATGAACAAGGCACACCTTTAACACCAATTTTGAGTTGTGGTATTTTTGGTGTCAAACTCGAAACTTCATTGGAAGTTTTGCTTGATGTTTGTAATACAAAAGAATTTAAAGTTTTTGTTTATACAGACACAGAGGTTTGTAAGGTTAAGGATTTTGTGTCTGGTTTAGTGAATGTTCAAAAAGTTGCACAACCTAAAATAGAACAAAAACCAGTGTCCTTAATTAAAGTTGCACCCAAGCCTTACAGGGTAGATGGTAAATTTAGTTACTTTACAGAAGACTTGTTGTGTGTCGCTGATGACAAACCCATTGTTTTGTTTACTGACTCTATGCTTACTTTGGATGACCGTGGTTTGGCTCTAGACAATGCACTTAGTGGTGTGCTTAGTGCTGCTATTAAGGATTGTGTTGACATAAATAAAGCTATACCTTCTGGTAATCTTATTAAGTTTGATATAGATTCTGTTGTTGTTTACATGTGTGTTGTGCCATCCGAAAAGGACAAACATTTAGATAATAATGTTCAACGATGCACACGTAAGTTGAATAGACTTATGTGTGATATAGTTTGTACTATACCAGCTGACTACATCTTGCCATTGGTGTTGTCTAGTTTGACTTGTAATGTTTCTTTTGTAGGTGAACTTAAAGCTGCTGAAGCTAAAGTTATAACTATAAAGGTGACAGAGGATGGTGTTAATGTTCATGATGTGACCGTGACAACAGATAAGTCATTTGAACAACAAGTTGGTGTTATTGCTGACAAGGACAAAGATCTTTCTGGTGCAGTACCCAGTGATCTTAACACATCTGAATTGCTTACTAAAGCAATAGATGTTGATTGGGTCGAATTTTATGGCTTTAAAGATGCTGTTACTTTTGCAACAGTTGATCATAGTGCTTTTGCCTATGAAAGTGCTGTTGTTAATGGTATTAGAGTGTTAAAAACCAGTGATAATAATTGTTGGGTGAATGCTGTTTGTATTGCACTACAGTATTCGAAACCCCATTTTATTTCACAAGGTCTTGATGCTGCGTGGAATAAATTTGTTTTAGGCGATGTTGAAATTTTTGTTGCATTTGTTTACTATGTTGCAAGACTAGTGAAAGGTGACAAGGGTGATGCTGAAGACACTTTGAATAAGTTGTCTAAGTATCTTGCTAATGAAGCTCAAGTTCAATTAGAACATTATAGTTCTTGTGTTGAATGTGATGCTAAATTTAAAAACTCTGTTACATCTATCAATTCTGCTATAGTTTGTGCTAGTGTCAAACGTGATGGTGTGCAAGTTGGTTATTGTGTCCATGGTATTAAGTACTATTCACGTGTTAAAAGTGTTAGAGGTAGAGCTATTATAGTCAGTGTCGAACAGCTTGAACCGTGTGTTCAGTCTAGACTTTTGAGTGGTGTTGCTTATACTGCTTTTTCTGGACCTGTTGATAAAGGTCATTATACTGTTTATGACACTGCAAAGAAATCAATGTATGATGGTGATCGTTTTGTTAAACATGATCTTTCTCTGCTGTCTGTCACATCAGTTGTTATGGTTGGTGATTATGTTGCACCTGTTAGTACAGTGAAACCTAAACCAGTCATTTCGCAACTTGATGAAAATGCACAGAAGTTCTTTGATTTTGGTGATTTTTTGATTCATAATTTTGTTATTTTTTTCACATGGTTATTGAGTATGTTTACTTTGTGTAAAACTGCAGTAACTACATGTGATGTTAAAATAATGGCCAAAGCACCACAAAGGACGGGTGTTGTTTTAAAACGTAGTCTTAAATATAACTTAAAAGCGTCAGCAGCTGTTCTTAAATCTAAGTGGTGGCTGCTTGCTAAGTTTACGAAACTACTGTTACTTATATATACATTGTATTCAGTAGTTTTGTTTTGTGTACGTTTTGGACCGTTTAAGTTTTGTAGTGAGACTGTTAATGGTTATGCTAAGTCAAACTTTGTCAAGGATGATTACTGTGATGGTTCATTGGGCTGCAAGATGTGTCTTTTTGGTTATCAAGAGTTAAGTCAATTTAGCCATTTGGATGTTGTGTGGAAGCATATAACAGACCCTTTGTTTAGTAATATACAACCTTTCATTGTCATGGTTTTGCTGCTTATATTTGGTGACAATTATTTGAGATGCTTCTTGCTGTATTTTGTTGCTCAGATGATAAGCACAGTTGGTGTTTTTCTAGGTTATAAGGAAACAAATTGGTTCTTGCACTTTATTCCATTTGATGTTATTTGTGATGAACTGCTTGTCACTGTTATTGTTATTAAGGTTATTTCTTTTGTCAGACATGTGCTTTTTGGTTGTGAAAACCCAGATTGCATTGCGTGTTCTAAGAGTGCTAGACTTAAGAGATTCCCTGTTAACACAATTGTTAATGGTGTGCAACGTTCATTTTATGTTAATGCAAATGGTGGTAGTAAGTTTTGTAAGAAACATAGATTTTTCTGTGTTGATTGTGACTCTTATGGTTATGGCAGCACGTTTATAACACCCGAAGTTTCTAGAGAACTTGGTAACATTACTAAAACAAATGTGCAACCAACAGGTCCAGCGTATGTCATGATTGACAAAGTGGAGTTTGAAAATGGTTTTTACAGGTTGTATTCCGGTGAAACATTTTGGCGTTACAACTTTGATATAACTGAAAGCAAGTATTCTTGCAAAGAGGTTTTTAAAAATTGTAATGTTTTGGATGATTTCATCGTGTTTAACAATAATGGGACCAATGTAACGCAGGTTAAAAATGCTAGTGTTTACTTTTCACAGTTGTTGTGTAGGCCCATTAAATTAGTTGACAGTGAACTTTTGTCCACTTTGTCAGTTGATTTTAATGGTGTCTTACACAAGGCATACATCGATGTACTACGTAATAGCTTTGGTAAAGATCTTAATGCTAATATGTCTTTAGCCGATTGCAAGAGTGCTTTAGGCCTGTCTATTAGTGATCATGAATTTACTAGTGCTATTTCTAATGCACATCGTTGTGACGTGTTGTTATCTGATTTGTCATTTAACAACTTTGTCAGTTCGTATGCTAAACCTGAGGAAAAATTATCAGCTTATGACTTGGCGTGTTGTATGCGTGCAGGTGCTAAGGTTGTTAATGCCAATGTTCTGACAAAGGACCAAACTCCTATTGTTTGGCATGCAAAGGATTTTAACAGTCTTTCTGCTGAAGGTCGTAAGTATATTGTAAAAACTAGCAAAGCTAAGGGTTTGACTTTCTTGTTGACAATTAATGAAAACCAAGCTGTCACGCAAATACCTGCAACTAGCATTGTTGCTAAGCAAGGTGCTGGTGATGCTGGTCATTCATTAACATGGCTGTGGCTACTGTGTGGTCTTGTGTGTTTGATTCAATTCTACTTGTGCTTTTTCATGCCCTATTTTATGTACGATGTCGTGAGTAGTTTTGAGGGTTATGATTTTAAGTATATAGAAAATGGTCAGTTGAAGAATTTTGAAGCGCCACTTAAATGTGTCAGAAACGTTTTTGAAAACTTTGAGGACTGGCATTATGCTAAGTTTGGCTTCACACCTTTAAACAAGCAAAGCTGTCCTATTGTAGTTGGAGTTTCTGAAATTGTTAATACTGTCGCTGGCATTCCATCTAATGTGTATCTTGTTGGTAAAACTTTAATTTTTACACTACAAGCTGCTTTTGGTAATGCTGGTGTTTGTTATGACATTTTTGGAGTCACAACACCTGAAAAGTGCATTTTTACTTCTGCTTGTACTAGATTAGAAGGTTTGGGTGGTAACAATGTTTATTGTTATAACACAGAGCTTATGAAAGGTTCTTTGCCTTACAGTTCAATACAAGCTAATGCATATTATAAATATGATAATGGCAATTTTATTAAGTTGCCAGAAGTTATTGCACAAGGCTTTGGCTTTAGAACAGTACGTACTATTGCTACCAAATACTGCCGCGTAGGTGAATGTGTTGACTCCAATGCAGGTGTGTGTTTTGGCTTTGACAAGTGGTTTGTTAATGATGGACGTGTTGCCAATGGTTACGTTTGTGGTACTGGTTTGTGGAACCTTGTATTTAACATACTTTCCATGTTCTCATCTTCATTCTCTGTTGCTGCAATGTCAGGTCAAATTTTACTTAATTGTGCATTAGGTGCTTTTGCTATCTTTTGTTGTTTTCTTGTGACAAAGTTTAGACGCATGTTTGGTGACCTTTCTGTAGGTGTTTGCACTGTTGTTGTGGCTGTTTTGCTTAACAATGTCTCTTACATTGTAACTCAGAATTTAGTAACAATGATTGCTTATGCCATATTGTATTTCTTTGCTACTAGAAGCTTACGCTATGCATGGATTTGGTGTGCTGCATATTTAATTGCGTATATTTCTTTTGCTCCATGGTGGTTGTGTGCTTGGTACTTTCTTGCTATGTTGACAGGTTTGTTACCTAGTTTGCTGAAGCTTAAAGTTTCGACAAATCTTTTCGAAGGTGACAAATTTGTAGGTACATTTGAAAGTGCTGCTGCAGGAACATTTGTCATTGACATTCGTTCTTATGAGAAACTTGCTAATAGCATCTCTCCAGAAAAGTTGAAAAGTTATGCTGCTAGCTATAATAGATATAAGTACTATAGTGGTAATGCAAATGAAGCTGATTACCGTTGTGCTTGTTATGCCTATTTAGCAAAAGCAATGTTGGACTTTTCGCGTGATCATAATGACATCTTGTACACACCTCCGACTGTCAGTTATGGTTCTACATTACAGGCTGGTTTGCGCAAAATGGCACAACCATCTGGCTTTGTGGAGAAATGTGTTGTCCGTGTCTGCTATGGAAACACTGTGTTGAATGGGTTGTGGCTTGGTGATATTGTTTATTGCCCACGTCATGTTATCGCATCTAACACAACTTCTGCTATAGATTATGATCACGAATATAGTATTATGCGGTTGCATAATTTTTCTATAATATCTGGTACAGCATTTCTTGGTGTTGTAGGTGCTACTATGCATGGAGTAACTCTTAAAATTAAGGTTTCACAGACTAACATGCACACACCTAGACACTCTTTTAGAACACTAAAATCTGGTGAAGGTTTTAACATCTTAGCATGCTATGATGGTTGTGCTCAAGGTGTTTTTGGTGTGAACATGAGAACTAATTGGACTATCCGTGGTTCATTTATTAATGGTGCGTGTGGTTCTCCTGGCTACAATCTTAAAAATGGCGAGGTGGAATTTGTTTATATGCATCAAATTGAACTCGGAAGTGGTAGCCATGTAGGTTCTAGCTTTGATGGTGTTATGTATGGTGGTTTTGAAGACCAACCTAATCTTCAAGTTGAATCTGCAAACCAGATGTTAACAGTTAATGTGGTTGCATTTCTTTATGCTGCTATATTGAATGGTTGCACATGGTGGCTTAAAGGTGATAAATTGTTTGTGGAGCATTATAATGAGTGGGCACAGGCTAATGGTTTCACAGCTATGAATGGTGAAGACGCTTTTTCCATTCTTGCTGCTAAAACTGGTGTCTGTGTGGAAAGATTACTTCATGCTATTCAAGTTTTGAATAATGGATTTGGTGGTAAACAAATTTTGGGTTATTCTAGTCTCAATGATGAGTTCAGTATTAATGAAGTTGTCAAACAAATGTTTGGTGTTAACCTGCAAAGTGGTAAAACCACTAGTATGTTTAAATCCATAAGCTTGTTTGCTGGCTTCTTTGTCATGTTCTGGGCTGAATTATTTGTTTATACCACCACTATTTGGGTTAACCCTGGTTTTCTTACTCCGTTTATGATTTTGCTTGTTGCTTTGTCACTCTGTCTTACATTTGTTGTTAAACATAAGGTTTTGTTTTTGCAAGTGTTTTTGTTGCCTTCAATTATTGTGGCTGCTATTCAAAACTGTGCTTGGGACTACCATATTACAAAGGTGTTGGCAGAGAAGTTTGATTATAATGTTTCTGTTATGCAAATGGACATCCAGGGTTTTGTTAACATTTTTATTTGTCTTTTTGTTGCACTGTTGCATACTTGGCGCTTTGCTAAAGAGCGTTGTACACATTGGTGCACTTATTTGTTCTCACTCATTGCTGTTTTATACACTGCATTGTATAGTTATGACTACGTTAGTTTGCTGGTTATGCTACTTTGTGCAATTTCTAATGAATGGTATATTGGTGCTATCATTTTTAGAATTTGTCGTTTTGGTGTTGCATTTTTACCAGTGGAATACGTGTCTTACTTTGATGGTGTTAAAACTGTGCTGTTGTTTTACATGTTGTTAGGCTTTGTTAGCTGTATGTACTATGGTTTGTTGTACTGGATTAACAGGTTCTGTAAGTGCACATTAGGTGTTTATGATTTCTGTGTTAGTCCAGCCGAATTTAAGTATATGGTTGCTAATGGTTTGAATGCACCAAATGGCCCTTTTGATGCACTCTTTCTGTCTTTTAAACTAATGGGTATTGGTGGTCCTAGAACCATTAAAGTCTCTACTGTACAGTCTAAATTGACTGATCTTAAGTGCACAAACGTCGTTCTAATGGGCATTTTGTCTAACATGAACATAGCTTCTAACTCAAAGGAGTGGGCATATTGTGTTGAAATGCACAATAAAATAAATTTGTGTGACGACCCTGAAACTGCTCAAGAGTTATTGCTGGCGTTGTTGGCCTTTTTCTTGTCTAAGCATAGTGATTTTGGTCTTGGTGATCTTGTCGATTCTTATTTTGAGAACGACTCCATTTTGCAAAGTGTTGCATCTTCTTTTGTTGGTATGCCATCTTTTGTTGCATATGAAACAGCAAGACAAGAGTATGAAAATGCTGTTGCAAATGGTTCCTCACCACAAATAATCAAACAATTGAAGAAGGCTATGAATGTTGCAAAAGCTGAGTTTGACAGGGAGTCATCTGTTCAAAAGAAAATTAACAGAATGGCTGAACAAGCTGCTGCAGCTATGTACAAAGAAGCACGTGCTGTTAATAGAAAATCAAAAGTTGTTAGTGCAATGCATAGTTTACTCTTTGGCATGCTCCGACGTTTGGACATGTCTAGTGTTGACACTATCCTTAATATGGCACGTAATGGTGTTGTCCCTCTTTCCGTTATCCCTGCTACTTCTGCAGCCAGGCTCGTCGTCGTAGTACCAGATCATGATTCATTTGTGAAAATGATGGTAGATGGTTTTGTGCACTACGCTGGTGTTGTTTGGACATTACAGGAAGTTAAGGATAATGACGGTAAGAATGTGCATCTTAAAGATGTTACAAAGGAAAACCAGGAAATACTTGTTTGGCCTCTGATTTTGACTTGTGAACGTGTCGTTAAATTGCAGAACAATGAAATAATGCCAGGCAAGATGAAGGTCAAGGCCACCAAAGGTGAAGGTGATGGAGGCATTATTAGTGAAGGTAATGCTCTATACAACAATGAAGGTGGACGTGCATTTATGTATGCATATGTGACTACGAAGCCTGGCATGAAGTATGTTAAATGGGAACATGACTCTGGTGTGGTTACAGTTGAATTGGAACCACCTTGCAGATTTGTTATAGACACACCTACCGGACCCCAAATTAAGTATCTTTATTTTGTTAAGAATCTTAACAATTTAAGGAGAGGTGCTGTTTTGGGTTACATTGGTGCCACTGTGAGATTGCAAGCTGGCAAACAGACTGAGTTTGTTTCAAACTCCCATTTATTAACACATTGTTCTTTTGCTGTTGACCCAGCTGCAGCCTACCTTGATGCTGTTAAACAAGGCGCAAAACCTGTTGGCAATTGTGTAAAGATGTTGACTAATGGTTCTGGTAGTGGTCAGGCTATTACTAGTACCATTGATTCTAACACTACGCAGGACACATATGGTGGCGCGTCTGTTTGTATTTATTGCAGAGCACATGTTGCACATCCAACCATGGACGGTTTTTGTCAGTACAAAGGTAAGTGGGTACAAGTGCCCATAGGTACAAATGACCCTATAAGATTTTGTCTTGAAAATACTGTTTGTAAAGTTTGTGGTTGTTGGCTTAATCATGGCTGTACATGTGACCGGACTGCTATCCAAAGTTTTGATAGCAGTTATTTAAACGAGTCCGGGGCTCTAGTGCCGCTCGACTAG
Protein
MACNRVTLAVASDSEISAYGCSTIAQAVRRYSEAASNGFRACRFVSLDLQDCIVGIADDTYVMGLHGNQTLFCNIMKFSDRPFMLHGWLVFSNSNYLLEEFDVVFGKRGGGNVTYTDQYLCGADGKPVISEDLWQFVDHFGENEEIVINGRTYVCAWLTKRKPLDYKRQNNLAIEEIEYVHGDALHTLRNGSVLEMAKEVKTSSKVVLSDALDKLYKVFGSPVMTNGSNILEAFTKPVFISALVQCTCGTKSWSVGDWTGFKSSCCNLISNKLCVVPGNVKPGDAVITTQQAGAGVKYFCGMTLKFVANIEGVSVWRVIALQSVDCFVASSTFVEEEHVNRMDTFCFNVRNSVTDECRLAMLGAEMTSNVRRQVASGVIDISTGWFDVYDDIFAESKPWFVRKAEDIFGPCWSALVSALKQLKVTTGELVRFVKSICNSAVAVVGGTIQILASVPEKFLNAFDVFVTAIQTVFDCAVETCTIAGKAFDKVFDYVLLDNALVKLVTTKLKGVREGGLNKVKYATVVVGSTEEVKSSRVERSNAVLTIANNYSKLFDEGYTVVIGDVAYFASDGYFRLMASPNSVLTTAVYKPLVDFNVNVMGTRPEKFPTTVTCENLESAVLFVNDKIIEFQLDYSIDVIDNEIIVKPNISLCVPLYVRDYVDKWDDFCRQYSNESWFEDDYKAFISVLDITDAAVKAAESKAFVDTIVPPCPSILKVIDGGKIWNGVIKNVNYVRDWLKSLKLNLTQQGLLGTCAKRFKRWLGILLEAYNAFLDTVVSTVKIGGLTFKTYAFDKPYIVIRDIVCKVENKTEAEWIELFPHNDRIKSFSTFESAYMPIADPTHFDIEEVELLDAEFVEPGCGGILSVIDEHVFYKKDGVYYPSNGTNILPVAFTKAAGGKVSFSDDVEVKDIEPVYRVKLCFEFEDEKLVDVCEKAIGKKIKHDGDWDSFCKTIQSALSVVSCYVNLPTYYIYDEEGGNDLSLPVMISEWPLSESDKEEDVQQAQQEATHIAEDVVDQVEEVNSIFDIETVDVKHEMSPFEMPFEELNGLKILKQLDNNCWVNSVMLQIQLTGILDGDYAMQFFKMGRVAKMIERCYTAEQCIRGAMGDVGLCMYRLLKDLHTGFMVMDYKCSCTSGRLEESGAVLFCTPTKKAFPYGTCLNCNAPRMCTIRQLQGTIIFVQQKPEPVNPVSFVVKPVCSSIFRGAVSCGHYQTNIYSQNLCVDGFGVNKIQPWTNDALNTICIKDADYNAKDEISVTPIKNTVDTIPKEKFVVKEKLNAFLVHDNVAFYQGDVDTVVNGVDFDFIVNAANENLAHGGGLAKALDVYTKGKLQRLSKEHIGLAGKVRVGTGVMVECDSLRIFNVVGPRKGKHERDLLVKAYNTINNEQGTPLTPILSCGIFGVKLETSLEVLLDVCNTKEFKVFVYTDTEVCKVKDFVSGLVNVQKVAQPKIEQKPVSLIKVAPKPYRVDGKFSYFTEDLLCVADDKPIVLFTDSMLTLDDRGLALDNALSGVLSAAIKDCVDINKAIPSGNLIKFDIDSVVVYMCVVPSEKDKHLDNNVQRCTRKLNRLMCDIVCTIPADYILPLVLSSLTCNVSFVGELKAAEAKVITIKVTEDGVNVHDVTVTTDKSFEQQVGVIADKDKDLSGAVPSDLNTSELLTKAIDVDWVEFYGFKDAVTFATVDHSAFAYESAVVNGIRVLKTSDNNCWVNAVCIALQYSKPHFISQGLDAAWNKFVLGDVEIFVAFVYYVARLVKGDKGDAEDTLNKLSKYLANEAQVQLEHYSSCVECDAKFKNSVTSINSAIVCASVKRDGVQVGYCVHGIKYYSRVKSVRGRAIIVSVEQLEPCVQSRLLSGVAYTAFSGPVDKGHYTVYDTAKKSMYDGDRFVKHDLSLLSVTSVVMVGDYVAPVSTVKPKPVISQLDENAQKFFDFGDFLIHNFVIFFTWLLSMFTLCKTAVTTCDVKIMAKAPQRTGVVLKRSLKYNLKASAAVLKSKWWLLAKFTKLLLLIYTLYSVVLFCVRFGPFKFCSETVNGYAKSNFVKDDYCDGSLGCKMCLFGYQELSQFSHLDVVWKHITDPLFSNIQPFIVMVLLLIFGDNYLRCFLLYFVAQMISTVGVFLGYKETNWFLHFIPFDVICDELLVTVIVIKVISFVRHVLFGCENPDCIACSKSARLKRFPVNTIVNGVQRSFYVNANGGSKFCKKHRFFCVDCDSYGYGSTFITPEVSRELGNITKTNVQPTGPAYVMIDKVEFENGFYRLYSGETFWRYNFDITESKYSCKEVFKNCNVLDDFIVFNNNGTNVTQVKNASVYFSQLLCRPIKLVDSELLSTLSVDFNGVLHKAYIDVLRNSFGKDLNANMSLADCKSALGLSISDHEFTSAISNAHRCDVLLSDLSFNNFVSSYAKPEEKLSAYDLACCMRAGAKVVNANVLTKDQTPIVWHAKDFNSLSAEGRKYIVKTSKAKGLTFLLTINENQAVTQIPATSIVAKQGAGDAGHSLTWLWLLCGLVCLIQFYLCFFMPYFMYDVVSSFEGYDFKYIENGQLKNFEAPLKCVRNVFENFEDWHYAKFGFTPLNKQSCPIVVGVSEIVNTVAGIPSNVYLVGKTLIFTLQAAFGNAGVCYDIFGVTTPEKCIFTSACTRLEGLGGNNVYCYNTELMKGSLPYSSIQANAYYKYDNGNFIKLPEVIAQGFGFRTVRTIATKYCRVGECVDSNAGVCFGFDKWFVNDGRVANGYVCGTGLWNLVFNILSMFSSSFSVAAMSGQILLNCALGAFAIFCCFLVTKFRRMFGDLSVGVCTVVVAVLLNNVSYIVTQNLVTMIAYAILYFFATRSLRYAWIWCAAYLIAYISFAPWWLCAWYFLAMLTGLLPSLLKLKVSTNLFEGDKFVGTFESAAAGTFVIDIRSYEKLANSISPEKLKSYAASYNRYKYYSGNANEADYRCACYAYLAKAMLDFSRDHNDILYTPPTVSYGSTLQAGLRKMAQPSGFVEKCVVRVCYGNTVLNGLWLGDIVYCPRHVIASNTTSAIDYDHEYSIMRLHNFSIISGTAFLGVVGATMHGVTLKIKVSQTNMHTPRHSFRTLKSGEGFNILACYDGCAQGVFGVNMRTNWTIRGSFINGACGSPGYNLKNGEVEFVYMHQIELGSGSHVGSSFDGVMYGGFEDQPNLQVESANQMLTVNVVAFLYAAILNGCTWWLKGDKLFVEHYNEWAQANGFTAMNGEDAFSILAAKTGVCVERLLHAIQVLNNGFGGKQILGYSSLNDEFSINEVVKQMFGVNLQSGKTTSMFKSISLFAGFFVMFWAELFVYTTTIWVNPGFLTPFMILLVALSLCLTFVVKHKVLFLQVFLLPSIIVAAIQNCAWDYHITKVLAEKFDYNVSVMQMDIQGFVNIFICLFVALLHTWRFAKERCTHWCTYLFSLIAVLYTALYSYDYVSLLVMLLCAISNEWYIGAIIFRICRFGVAFLPVEYVSYFDGVKTVLLFYMLLGFVSCMYYGLLYWINRFCKCTLGVYDFCVSPAEFKYMVANGLNAPNGPFDALFLSFKLMGIGGPRTIKVSTVQSKLTDLKCTNVVLMGILSNMNIASNSKEWAYCVEMHNKINLCDDPETAQELLLALLAFFLSKHSDFGLGDLVDSYFENDSILQSVASSFVGMPSFVAYETARQEYENAVANGSSPQIIKQLKKAMNVAKAEFDRESSVQKKINRMAEQAAAAMYKEARAVNRKSKVVSAMHSLLFGMLRRLDMSSVDTILNMARNGVVPLSVIPATSAARLVVVVPDHDSFVKMMVDGFVHYAGVVWTLQEVKDNDGKNVHLKDVTKENQEILVWPLILTCERVVKLQNNEIMPGKMKVKATKGEGDGGIISEGNALYNNEGGRAFMYAYVTTKPGMKYVKWEHDSGVVTVELEPPCRFVIDTPTGPQIKYLYFVKNLNNLRRGAVLGYIGATVRLQAGKQTEFVSNSHLLTHCSFAVDPAAAYLDAVKQGAKPVGNCVKMLTNGSGSGQAITSTIDSNTTQDTYGGASVCIYCRAHVAHPTMDGFCQYKGKWVQVPIGTNDPIRFCLENTVCKVCGCWLNHGCTCDRTAIQSFDSSYLNESGALVPLD
Summary
Function
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Catalytic Activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
Proteomes
PRIDE
Pfam
Interpro
IPR037230
NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR014829 NSP8
IPR011050 Pectin_lyase_fold/virulence
IPR036499 NSP9_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR023298 ATPase_P-typ_TM_dom_sf
IPR032505 Corona_NSP4_C
IPR036333 NSP10_sf
IPR014828 NSP7
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR013016 Peptidase_C30/C16
IPR014822 NSP9
IPR009003 Peptidase_S1_PA
IPR014829 NSP8
IPR011050 Pectin_lyase_fold/virulence
IPR036499 NSP9_sf
IPR018995 RNA_synth_NSP10_coronavirus
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR023298 ATPase_P-typ_TM_dom_sf
IPR032505 Corona_NSP4_C
IPR036333 NSP10_sf
IPR014828 NSP7
SUPFAM
ProteinModelPortal
PDB
2ZU2
E-value=0.0 Score= 633 Identity=99.67%
Cov(Q)=7.38% Cov(P)=100.00%
3D Structure
Ontologies
GO
GO:0039520 P:induction by virus of host autophagy
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0004197 F:cysteine-type endopeptidase activity
GO:0019079 P:viral genome replication
GO:0033644 C:host cell membrane
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0016021 C:integral component of membrane
GO:0008242 F:omega peptidase activity
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0004197 F:cysteine-type endopeptidase activity
GO:0019079 P:viral genome replication
GO:0033644 C:host cell membrane
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0016021 C:integral component of membrane
GO:0008242 F:omega peptidase activity
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0039548 P:suppression by virus of host IRF3 activity
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 3 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 3 publications.
Topology
Length:
4090
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
428.19346
Exp number, first 60 AAs:
0.0061
Total prob of N-in:
0.00335
outside
1 - 1933
TMhelix
1934 - 1956
inside
1957 - 1993
TMhelix
1994 - 2016
outside
2017 - 2071
TMhelix
2072 - 2094
inside
2095 - 2100
TMhelix
2101 - 2119
outside
2120 - 2497
TMhelix
2498 - 2520
inside
2521 - 2725
TMhelix
2726 - 2745
outside
2746 - 2754
TMhelix
2755 - 2777
inside
2778 - 2783
TMhelix
2784 - 2801
outside
2802 - 2804
TMhelix
2805 - 2824
inside
2825 - 2830
TMhelix
2831 - 2853
outside
2854 - 3036
TMhelix
3037 - 3056
inside
3057 - 3282
TMhelix
3283 - 3305
outside
3306 - 3309
TMhelix
3310 - 3329
inside
3330 - 3335
TMhelix
3336 - 3358
outside
3359 - 3372
TMhelix
3373 - 3395
inside
3396 - 3407
TMhelix
3408 - 3430
outside
3431 - 3439
TMhelix
3440 - 3459
inside
3460 - 3470
TMhelix
3471 - 3493
outside
3494 - 4090
