Coronavirus Database

Strain

Bat_KJ473812 (Region: China; Strain: Severe acute respiratory syndrome-related coronavirus; Date: 2013)

Gene

ORF1ab polyprotein

Description

Annotated in NCBI, ORF1ab polyprotein

Location

KJ473812(+):8-21213

GenBank Accession

AIA62289.1

Full name

Replicase polyprotein 1ab

Alternative Name

ORF1ab polyprotein

CDS

ATGGAGAGCCTTGTTCTTGGTGTCAACGAGAAAACACACGTCCAACTCAGTTTACCTGTTCTTCAGGTTAGTGACGTGTTAGTGCGTGGTTTCGGGGATACTGTGGAAGAAGCCCTAGCGGAAGCACGTGAACATCTTAAAAATGGCACTTGTGGCCTAGTAGAGCTGGAAAAAGGTGTTTTACGCCAGCTTGAAGAGCCCTATGTGTTCATTAAACGATCTGAAGCCTTAAGCACCACTCATGGCCATAAGGTTGTTGAATTGGTGGCTGAAATGAATGGCATTCAGTTCGGTCGTAGCGGTACAACACTGGGAGTTCTCGTGCCACATGTGGGCGAAACCCCAATTGCGTACCGCAATGTTCTTCTTCGCAAGAACGGTAATAAGGGAGCTGGTGGTCATAGCTACGGCATCGATCTAAAGTCTTATGACTTAGGTGACGAGCTTAACACTGATCCCATTGAAGATTATGAACAAAAATGGAACACTAAGCATGGCAGGGGTGCTCTCCGTGAACTCATTCGTGAGCTTAATGGAGGTGCAGTCACTCGCTATGTTGATAACAACTTCTGTGGCCCTGATGGGTATCCTCTTGACTGCATTAAAGATCTTCTCGCTCGTGCGGGTAAGTTGATGTGCACTCTTTCCGAACAACTTGATTTCATTGAATCGAAGCGTGGTGTCTATTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACTGAGCGCTCAGACAAGAGTTATGAGCACCAGACACCATTCGAGATTAAGAGTGCCAAGAAATTTGACACTTTTAAAGGGGAATGCCCGAAGTTCGTATTTCCTCTCAACTCTAAAGTCAAAGTCATTCAACCACGTGTTGAAAAGAAAAAGACTGAAGGTTTCATGGGGCGTATACGCTCTGTGTACCAAGTTGCCACTCCAAATGAATGCAACGACATGCACTTATCTGTCTTTATGAAGTGCAATCATTGCGATGAAGCTTCTTGGCAGACGTGTGATTTTCTCAAAGCCACTTGTGAATATTGTGGCACTGAAAATCCAGTTAGTGAAGGACCTACTACATGTGGGTACCTACCTACTAATGCTGTAGTAAAGATGCCCTGTCCCGCTTGTCAGAATAAAGAAGTGGGACCTGAGCATAGTGTTGCGGACTACCACAACCACTCAAACATTGAAACTCGACTCCGCAAGGGAGGTAGGACTAAATGTTTTGGAGGCTGTGTGTTTTCCTATGTTGGCTGCTATAACAAGCGTGCTTACTGGGTTCCTCGTGCTAGTGCCGATATAGGTTCAAACCATACTGGCATATCTGGTGACAATGTAGAAGTTCTTAATGAAGACCTCCTTGAGATACTGAATCGTGAACGTGTTAATATTAACATTGTTGGCGATTTTCAGTTGAATGAAGAGATTGCCATCATTTTAGCGTCTCTTTCTGCTTCTACGAGTGCTTTTGTTGACACTGTAAAGAGTCTTGATTACAAGTCTTTTAAAGCCATTGTTGAGTCTTGCGGAAACTACAAAGTTACCAAGGGCAAGCCTGTGAAAGGTGCTTGGAACATTGGACAACAAAAATCTATTCTGACACCACTGTGTGGATTTCCATCACAGGCTGCTGGTGTTATTAGATCTATTTTCTCTCGCACACTAGATGCAGCAAATAATTCTATCCCAGATTTACAAAGAGCAGCTGTCACCATCCTTGGTGACATTTCTGAACAGTCACTGCGTCTTGTTGATGCAATGGTGTGTACATCAGACTTGATTACCAACAGTGTTATCATCATGGCATATGTTACTGGTGGTCTTGTACAGCAAACAGTGCAATGGTTGTCTAATGTGTTAGGCACCACAGTCGACAAACTCAAACCTGTATTTACATGGCTTGAGACTAAGCTTAATGCAGGAATAGAGTTTCTCAAGGATGCTTGGGAAATTCTTAAATTCCTAGTTACAGGTGTGTTTGACATTGTTAAAGGTCAAATACGGGTGTTTTCAGACAACCTCAAAGAATGTGTAAAAACTTTTGTTGGTGTTGTCAATAAAGCGCTTGAAATGTGCATTGACCAAGTCACAATCGCAGGCACCAAGGTGAGATCACTCAACCTTGGAGAGGTTTTTATTGCGCAAAGCAAGGGCCTCTATCGTCAGTGTGTTCGTGGCAAGGAGCAGCTGCAACTACTCATGCCTTTGAAGGCACCTAAAGAAGTCACTTTCCTTGAAGGAGACTCACATGATACAGTACTAATCTCCGAAGAGGTTGTTCTTAAAAATGGTGAGCTTGAAGCACTCGAGACACCAGTTGACAGTTTCATTAATGGAGCCGTCGTAGGCAATCCAGTTTGTGTCAATGGCCTCATGCTCTTGGAGCTCAAAGATAAGGAACAGTATTGTGCTTTATCTCCAGGCTTATTAGCTACAAACAATGTTTTCCGTCTAAATGGAGGTGCACCGGTTAAAGGTGTAACCTTTGGAGAAAACACTGTTTTAGAAGTTCAAGGTTACAAGAATGTGAAAATCACATTTGAGCTTGATGAGCGTGTAGACAAAGTGCTTAATGAGAAGTGTTCTGTCTACACTGTTGAATCCGGTACAGAGGTTACTGAATTCGCATGTGTTGTTGCAGAAGCCGTTGTAAAAACTTTGCAACCAGTTTCTGACCTTCTTACTAAGATGGGTATTGACCTTGACGAGTGGAGTGTAGCTACATTCTACTTGTTTGATGATGCTGGTGAAGAAAAACTTTCATCACGCATGTACTGTTCCTTCTACCCTCCTGATGAGGAAGAAGACTGCGAAGAGTATGAGGGTGAGGAAGAAGTTCCTGATGGAACCTGTGAACATGAATACGGCACAGAGGATGACTATAAAGGTCTTCCTCTTGAGTTTGGTTCGTCAACAGAAATACAACAGGTAGACGATGAAGAGGAAGACTGGCTTGATGATGCTGGTGAAGCAGAACCTGAACCAGAACCTCTACCTGAAGAACCAGTTAATCAGTTTACTGGTTACTTTAAACTCACTGACAATGTTGCCATTAAATGTGTTGACATCGTTAAGGAAGCGCAAAGTGCCAAACCGGCGGTGATTGTTAACGCTGCTAACATCCACCTAAAACATGGTGGTGGTGTAGCAGGTGCACTCAACAAGGCAACTAATGGTGCCATGCAACAGGAGAGTGATGATTACATTAAACGAAATGGACCACTCACAGTAGGTGGTTCATGTTTGCTTTCTGGACACAATTTGGCTAAGAAGTGTATGCATGTTGTTGGCCCAAACCTAAATGCTGGTGATGATGTCCAACTACTTAAAGCTGCATATAACAACTTTAATTCACAGGACGTATTACTTGCACCACTACTGTCAGCAGGCATATTTGGTGCTAAACCACTTCAGTCTTTAAAGATGTGTGTTGAAGTAGTTCGCACACAAGTTTACCTCGCAGTCAATGATAGGAGTCTTTATGATCAGGTTGTATTAGATTATCTGGACAGTTTGAAACCTAAAGTGGAGTCTCCCAAGAAGGAGGAGAATCCAAAATTGGAAGAGCCTAAAGCGAAACAGCCAGCTGAAAAACCTGTTGACGTTAAACCTAAAATTAAGGCTTGTGTCGAAGAGGTTACTACAACATTGGAAGAAACTAAACTTCTTACTCGAAACCTGCTTCTTTTTGCTGATATCAATGGTAAACTTTACCCGGATTCTCAGAATATGTTAAGAGGTGAAGATATGTCTTTTCTAGAAAAGGACGCACCATATGTAGTAGGTGATGTCATCACTAGTGGTGATATTACTTGTGTTATAATACCTGCTAAGAAGGCTGGTGGTACTACAGAAATGCTTGCAAAGGCATTAAAGAAAGTGCCAGTGTGTGAGTATATAACTACATATCCCGGACAAGGATGTGCTGGTTATACACTCGAAGAAGCAAAGACTGCGCTTAAAAGGTGCAAATCTGCATTCTATGTATTACCTTCAAAAACACCTAATGTAAAAGATGAAATCCTTGGAACGGTGTCCTGGAATTTAAGGGAAATGCTTGCTCATGCTGAAGAGACAAGAAAATTAATGCCTATTTGCATGGACATTAGAGCCATAATGGCTACAATCCAACGTAAGTATAAAGGTATTAAAATTCAAGAAGGAATCGTTGACTATGGTGTTCGGTTCTTTTTCTATACTAGCAAAGAACCTGTAGCTTCTATTATTACAAAGCTCAATTCTTTAAATGAACCACTTATCACAATGCCGATAGGTTATGTGACACATGGTTTTAACCTGGAAGAAGCTGCGCGTTGTATGCGCTCTCTTAAAGCTCCTGCTGTAGTTTCAGTGTCTTCACCAGACGCAGTTACTACATACAATGGATACCTCACATCATCTTCAAAGACACCTGAGGAACACTTCATAGAGACCATCTCCCTTGCGGGTACGTATAGAGACTGGTCTTACTCTGGACAACGTACAGAATTAGGTGTTGAATTTCTCAAGCGTGGAGATAAGATTGTCTACCACACTATTGAAAAACCCATCGAATTTCATCTTGACGGTGAGGTTCTCCCACTTGACAAGCTCAAGAGTCTTTTGTCTCTTCGTGAGGTTAAGACTATTAAAGTGTTTACTACTGTAGACAATACTAACCTCCACACACAACTTGTGGACATGTCTGTGACATATGGACAGCATTTCGGTCCAACCTATTTGGACGGTGCTGATGTCACTAAGGTTAAGCCTCATGTTAATCATGAGGGTAAGACTTTCTTTGTACTACCTAGTGATGACACACTGCGTAGTGAAGCATTTGAATATTACCACACTCTCGACGAGAGTTTTCTCGGTAGATATATGTCTGCTTTGAGTCACACAAAGAAATGGAAATTTCCTCAAGTTGGTGGTTTAACTTCAATCAAATGGGCTGACAATAACTGTTATTTGTCCAGTGTTTTATTAGCACTTCAGCAGATTGAGGTGAAGTTTAATGCACCCGCACTACAAGAAGCCTATTATAGAGCTCGTGCTGGTGATGCTGCTAATTTTTGTGCGCTCATACTTGCTTACAGTAATAAAACTGTGGGTGAGTTGGGTGATGTTAGGGAAACTATGACCCATCTTCTACAACATGCCAACTTGGAATTCGCTAAGAGGGTTCTTAATTTGGTGTGTAAGCATTGCGGACAGAAAACTACCACCTTAACGGGTGTAGAAGCCGTGATGTACATGGGTACTTTGTCTTATGATGAGCTTAAGACAGGTGTTTCAATTCCTTGTGTGTGTGGTCGTGGTGCTACACAATATCTAGTACAACAAGAGTCTTCTTTTGTTATGATGTCTGCACCTCCTGCTGAGTACAAGTTGCAGCAGGGTACATTTTTATGCGCTAATGAGTACACTGGTAATTATCAGTGTGGTCATTATACTCATATAACTGCCAAGGAAACGCTCTATCGTATAGATGGAGCTCACCTTACAAAAATGTCAGAATATAAAGGACCAGTGACTGATGTTTTCTATAAAGAAACATCTTACACTACAACTATTAAGCCTGTGTCATATAAACTCGACGGAGTTACTTACACAGAGATTGAACCTAAATTAGATGGGTATTATAAAAAGGATAATGCTTACTACACTGAGCAGCCTATTGATCTTGTGCCAACTCAACCATTGCCAAATGCGAGTTTTGACAATTTCAAACTCACATGCTCTAACATTAAGTTTGCTGATGACCTTAATCAAATGACAGGCTTTAAAAAGCCAGCTTCACGTGAGCTATCTGTCACATTCTTTCCAGACTTGAATGGCGATGTAGTGGCTATTGACTATAGACATTACTCAGCGAGTTTCAAGAAAGGTGCTAAACTGCTACATAAGCCAATTATTTGGCATATCAATCAGACTACAAACAAGACAACTTACAAGCCAAACACTTGGTGTTTACGTTGTCTTTGGAGTACAAAGCCAGTTGAAACCTCAAATTCATTTGAAGTTCTGGAAGTAGAAGGCACACAAGGAATGGATAATCTTGCTTGTGAAAGCCAACCACCAACCTCTGAAGAAGTAGTGGAAAATCCTACCGTACAGAAGGAAGTAATAGAGTGTGACGTGAAAATTACCGAAGTTGTAGGCAATGTCATACTAAAACCATCAGACGAAGGTGTCAAAGTGACACAAGAGTTAGGCCATGAAGATCTAATGGCTGCTTATGTAGAAAATACAAGCATTACCATTAAGAAACCTAATGAGCTCTCGTTGGCCTTAGGTTTAAAAACACTTGCCACTCATGGTGCTGCTGCAATCAATAGTGTCCCGTGGAGTAAGGTTTTGGCTTATGTTAAGCCTTTTCTAGGACAAGCAACAGTCACAACATCCAACTGCATGAAGAAATGTGTGCAGCGCATTTTTAACAACTATATGCCTTATGTCATTACATTATTATTCCAGTTGTGCACTTTCACAAAGGGCACCAACTCAAGAATTAGAGCATCACTTCCTACAATTATTGCTAAAAATAGTGTTAAGAGTGTTGCAAAATTATGTTTGGACGTTTGCATTGATTATGTGAAATCTCCTAAATCTTCTAAATTGTTCACAATTGCAATGTGGCTATTGTTGTTAAGCATTTGCTTGGGTTCATTAATCTATGTGACTGCAGCTTTTGGTGTGCTTTTATCTAATTTAGGCATCCCTTCTTATTGTGGTGGTGTTAGAGATTTGTATATCAATTCATCTAATGTCACCATTATGGACTTCTGTGAGGGTTCTTTTCCTTGTAGTGTTTGTTTAAGTGGACTAGATTCTCTTGATTCTTACCCCGCTTTAGAAACTATTCAGGTTACGATTTCATCGTATAAGTTGGACCTAACATTTTTGGGTTTAGCAGCTGAATGGTTTTTGGCATACATGTTGTTTACAAAATTTTTCTACTTACTTGGTCTCTCTGCTATAATGCAGGTGTTCTTTGGCTACTTTGCTAGTCATTTCATCAGCAATTCATGGCTTATGTGGTTTATCATTAGCATTGTACAAATGGCGCCTGTTTCTGCTATGGTCAGGATGTACATTTTCTTTGCTTCTTTCTATTATATATGGAAAAGCTATGTTCATATTATGGATGGTTGCACTTCTTCAACGTGCATGATGTGCTACAAGCGCAATCGTGCTACACGCGTTGAGTGTACAACTATAGTTAATGGCATGAAAAGATCTTTCTATGTCTATGCAAACGGAGGCCGTGGCTTCTGTAAGGCTCACAATTGGAACTGTCTTAATTGTGATACATTCTGTACTGGTAGTACTTTCATCAGCGACGAAGTAGCTCGTGATTTGTCACTCCAGTTTAAGAGACCAATTAATCCTACAGACCAGTCTTCTTATGTTGTTGATAGTGTCGCTGTGAAGAACGGTGCACTCCATCTCTACTTTGATAAGGCTGGTCAGAAGACTTACGAGAGACACCCACTTTCTCTTTTTGTCAATTTGGACAATCTGAGAGCTAACAACACTAAGGGTTCATTACCTATTAATGTTATTGTTTTTGACGGCAAGTCCAAGTGCGAAGAGTCTGCCGCCAAGTCTGCATCTGTTTATTACAGTCAGCTTATGTGCCAACCTATTCTGTTACTTGACCAAGCTCTTGTTTCGGATGTTGGTGATAGTACCGAGGTTTCTGTCAAGATGTTTGATGCTTATGTCGACACCTTTTCAGCAACTTTTAGTGTTCCTATGGAAAAACTTAAGGCACTAGTGGCCACGGCTCATAGTGAGTTGGCTAAGGGTGTTGCTTTGGATGGTGTCTTATCCACATTTGTTTCAGCTGCCCGTCAAGGCGTTGTTGATACTGATGTTGATACAAAGGACGTCATTGAATGTCTCAAGCTTTCTCATCACTCTGACTTAGAAGTGACAGGTGACAGCTGTAATAACTTCATGCTCACCTATAACAAAGTTGAAAACATGACGCCCAGAGATCTTGGTGCATGTATTGATTGTAATGCAAGGCACATTAATGCCCAAGTAGCAAAAAGTCACAATGTCTCGCTCATCTGGAACGTCAAAGACTATATGTCATTGTCTGAACAGTTGCGCAAGCAAATTCGTAGTGCTGCTAAAAAGAACAACATACCCTTTAGGCTCACTTGTGCTACTACTAGGCAAGTTGTCAACGTCATAACTACTAAAATTTCACTCAAGGGTGGTAAGGTTGTTAGTACTTGGTTTAAATTTGTGCTGAAAGTCACACTTTTGTGTGTTCTATCTGCATTATTCTGTTACATCATTATGCCAGTACACTCATTGTCTGTTCATGATGGCTATACAAATGAAATCATTGGATACAAAGCTATCCAGGACGGTGTCACTCGTGACATAGTGTCTACTGATGATTGTTTTGCAAACAAACATGCTGGTTTCGACTCTTGGTTTAGCCAGCGTGGTGGTTCTTATAGGAATGATAAGAGCTGCCCTGTTGTAGCTGCCATCATTACTAGGGAGATTGGCTTCATCGTGCCTGGATTACCTGGTACTGTGTTAAGAACAATTAATGGTGACTTTTTGCATTTTCTACCTCGCGTTTTTAGTGCCGTTGGCAACATTTGCTACACACCATCAAAACTTATTGAGTATAGTGATTTTGCTACTTCTGCTTGCGTCTTGGCTGCGGAATGTACCATTTTTAAGGATGCTATGGGTAAGCCTGTGCCATATTGTTATGACACTAACTTACTAGAGGGTTCTATTTCTTACAGTGAGCTGCGTCCTGACACTCGTTATGTGCTTATGGATGGCTCTATCATACAATTCCCTAACACCTACCTAGAAGGTTCTGTTAGAGTGGTTACAACTTTTGATGCAGAGTACTGCCGTCATGGCACATGTGAGAGGTCAGAAGCTGGTATTTGCCTGTCTACTAGTGGTAGATGGGTTCTTAATAATGAACACTATAGGGCTCTGCCAGGAGTCTTTTGTGGTGTTGATGCCATGAATCTTATTGCTAACATCTTCACACCTCTTGTTCAACCTGTTGGTGCTTTAGATGTATCTGCTTCTGTAGTGGCGGGTGGTATTATTGCCATATTGGTGACTTGTGCTGCTTACTACTTTATGAAATTCAGGCGTGCATTTGGTGAGTACAACCATGTTGTAGCTGCTAATGCATTACTGTTTTTGATGTCTTTCACTATACTCTGTTTGGCACCTGCCTATAGTTTTTTGCCAGGAGTTTATTCTGTCTTTTACTTGTACTTGACATTCTATTTTACTAATGATGTTTCATTTTTAGCTCACCTTCAATGGTTTGCTATGTTTTCTCCCATTGTGCCTTTCTGGATAACAGCCATTTATGTGTTCTGCATCTCCTTGAAGCACTGCCACTGGTTCTTTAATAACTACCTTAAGAGAAGAGTCATGTTTAATGGAGTTACATTTAGCACCTTTGAGGAGGCTGCTTTATGTACCTTTTTACTTAACAAGGAAATGTACCTCAAATTGCGTAGTGAGACACTCTTGCCACTTACACAGTACAACAGGTACCTTGCTCTCTATAATAAGTACAAGTATTTCAGTGGGGCCTTGGACACAACCAGTTATCGTGAAGCAGCTTGCTGCCACTTAGCAAAGGCTCTTAATGACTTCAGTAACTCCGGTGCTGACGTCCTCTACCAACCACCACAAACTTCAATCACATCTGCAGTTTTGCAGAGTGGTTTTAGAAGAATGGCATTCCCGTCTGGTAAGGTTGAAGGGTGCATGGTTCAAGTTACTTGTGGAACCACAACTCTTAACGGATTGTGGTTAGATGACACAGTATATTGTCCAAGACATGTTATTTGCACAGCAGAAGACATGCTTAATCCAAACTATGAAGACCTGCTCATCCGTAAGTCTAACCATAGCTTCCTTGTCCAGGCTGGTAATGTCCAACTTCGTGTCATCGGCCATTCCATGCAAAATTGTCTGCTTAGGCTTAAAGTTGATACCTCTAACCCTAAGACACCAAAGTATAAATTTGTCCGTATCCAACCAGGTCAGACATTTTCAGTCTTAGCTTGTTACAATGGTTCACCATCAGGAGTCTACCAGTGTGCCATGAGACCTAACTACACCATTAAGGGTTCTTTCCTCAATGGGTCATGTGGTAGTGTTGGTTTTAACATTGACTATGATTGCGTGTCCTTTTGCTATATGCATCACATGGAGCTTCCAACTGGAGTACACGCTGGCACCGACTTAGAAGGTAAATTCTATGGTCCCTTTGTTGACAGACAAACTGCACAGGCTGCAGGCACAGACACAACCATTACATTGAACGTTTTGGCTTGGCTCTATGCTGCTGTTATTAATGGAGATAGATGGTTCCTTAATAGGTTTACCACAACTCTGAATGATTTTAATCTTGTGGCAATGAAATACAACTATGAGCCACTGACACAAGATCATGTTGACATACTGGGACCTCTTTCTGCACAAACAGGAATAGCTGTCTTAGATATGTGTGCTGCTCTAAAAGAGCTTCTACAGAATGGTATGAATGGTCGTACTATTCTTGGTAGCACTATTCTAGAAGATGAGTTTACGCCTTTCGATGTTGTTAGACAATGTTCTGGTGTGACTTTTCAAGGTAAGTTCAAGAAAATAGTTAAGGGCACTCATCATTGGATGCTCCTTACTTTCTTGACATCACTTTTAATTCTCGTTCAAAGTACACAGTGGTCACTGTTTTTCTTTGTTTATGAGAATGCTTTCTTGCCATTTACTCTTGGTATTATGGCTATTGCTGCTTGTGCTATGCTTCTGGTCAAGCATAAACATGCCTTCCTGTGCTTGTTTCTATTACCTTCTCTTGCAACAGTTGCTTATTTCAATATGGTCTACATGCCTGCTAGCTGGGTGATGCGTATTATGACATGGCTCGAATTGGCTGATACTAGCTTGTCTGGTTATCGGCTTAAAGACTGTGTTATGTATGCGTCAGCCTTAGTACTACTCACCCTCATGACTGCTCGTACTGTCTATGATGATGCTGCTAGGCGTGTATGGACATTGATGAATGTCATTACACTTGTTTATAAAGTCTATTATGGTAATTCCTTAGATCAAGCTATTTCCATGTGGGCTCTTGTTATTTCTGTAACCTCTAACTATTCTGGTGTCGTTACGACTATCATGTTCTTAGCTAGAGCTATAGTTTTTGTGTGTGTTGAGTATTACCCTTTCTTGTTCATTACTGGCAACACCTTACAGTGTATCATGCTTGTTTATTGTTTCTTAGGCTATTGTTGCTGTTGCTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTTACTCTTGGTGTTTATGACTATTTGGTCTCCACACAGGAGTTTAGATATATGAACTCGCAAGGGCTTTTGCCACCTAAGAGTAGTATTGATGCATTCAAGCTTAACATTAAATTGCTGGGCATTGGAGGAAAACCATGCATAAAAGTTGCTACTGTTCAGTCTAAAATGTCTGACGTGAAGTGCACTTCTGTTGTACTACTTTCTGTTCTTCAACAACTTAGAGTAGAATCATCTTCTAAATTGTGGGCACAATGTGTGCAATTGCACAATGACATTTTATTGGCTAAAGACACAACTGAAGCCTTTGAAAAGATGGTTTCTCTTCTGTCTGTTCTGCTATCTATGCAAGGTGCTGTAGACATCAACAAACTGTGCGAGGAAATGCTCGACAACCGCGCTACCCTGCAGGCTATTGCTTCAGAATTTAGTTCTTTACCATCATATGCTGCCTATGCTACAGCTCAAGAGGCTTATGAGCAGGCGGTAGCAAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTAAAGAAATCTTTGAATGTGGCTAAATCTGAGTTTGACCGTGATGCTGCCATGCAACGTAAGTTGGAGAAGATGGCGGACCAGGCTATGACCCAAATGTACAAACAGGCAAGATCTGAAGACAAGAGGGCAAAAGTTACTAGTGCAATGCAGACAATGCTTTTCACTATGCTTAGGAAGCTAGATAATGATGCACTTAACAACATTATCAATAATGCACGTGATGGTTGTGTACCACTCAACATCATACCACTTACAACAGCAGCTAAACTTATGGTTGTTGTACCTGATTATGGAACCTACAAGAATACTTGTGATGGTAACACTTTTACATATGCATCTGCTCTCTGGGAAATTCAGCAAGTTGTTGATGCAGATAGTAAAATTGTCCCGCTTAGTGAAATTAATATGGATAATTCACCAAATCTAGCTTGGCCTCTTATTGTTACTGCACTAAGAGCCAACTCAGCTGTCAAACTACAGAATAATGAACTGAGTCCAGTAGCACTACGACAGATGTCTTGTGCAGCTGGTACTACACAAACAGCTTGTACTGATGACAATGCACTTGCCTATTATAACAACTCTAAGGGAGGTAGGTTTGTGCTTGCATTACTATCAGACCATCAAGATCTCAAGTGGGCTAGATTCCCTAAGAGTGACGGAACAGGTACTATTTATACAGAACTGGAACCACCTTGTAGGTTCGTTACAGACACCCCAAAAGGACCTAAAGTGAAATACTTGTATTTCATTAAGGGCCTAAACAACCTAAATAGAGGTATGGTATTGGGCAGTTTAGCTGCTACAGTACGTCTTCAGGCTGGTAATGCAACTGAAGTGCCAGCCAACTCAACTGTGCTTTCTTTCTGCGCCTTTGCTGTTGATCCAGCTAAAGCATACAAAGATTACCTATCAAGTGGAGGACAACCAATCACCAATTGCGTGAAGATGCTGTGCACACACACTGGTACAGGACAGGCAATTACCGTAACACCCGAAGCCAATATGGATCAAGAGTCCTTTGGTGGTGCTTCATGTTGTCTGTATTGTAGATGCCACATTGATCATCCAAATCCTAAGGGATTTTGTGACCTGAAAGGTAAGTATGTTCAAATACCTACCACCTGTGCTAATGACCCAGTGGGTTTCACACTTAGAAACACAGTCTGTACTGTCTGCGGAATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGATGCAGTCTGCGGATGCGTCAGCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGCACTGATGTCGTTTACAGGGCTTTTGATATTTACAACGAAAAGGTTGCTGGTTTTGCAAAGTTCCTAAAAACTAATTGCTGCCGATTCCAAGAGAAGGATGAAGAAGGCAATTTAATAGACTCTTATTTCGTAGTTAAGAGACATACTATGTCCAACTACCAGCATGAAGAGGCTATTTACAACTTGCTTAAAGAGTGCCCAGCTGTTGCTGTGCATGACTTTTTCAAGTTTAGAGTAGATGGTGACATGGTACCACATATATCACGTCAACGTCTAACTAAGTACACAATGGCAGACTTAGTCTATGCTCTACGTCATTTTGACGAGGGCAATTGTGACACATTAAAGGAAATACTCGTCACATACAATTGTTGTGATGACGATTATTTCAATAAGAAGGATTGGTATGACTTTGTAGAAAACCCTGACATCCTGCGCGTATATGCAAACCTTGGTGAGCGTGTACGTCAAGCTTTATTAAAGACTGTGCAATTCTGCGATGCCATGCGCGATGCGGGTATCGTAGGTGTACTGACACTAGATAATCAGGATCTGAATGGGAACTGGTACGATTTCGGTGATTTCGTGCAAGTAGCACCAGGCTGCGGAGTTCCTATTGTGGATTCATACTATTCTTTGCTGATGCCTATTCTCACACTAACGAGGGCTTTAGCTGCTGAGTCCCATATGGACGCTGATCTCACAAAACCACTCATTAAGTGGGATTTGTTGAAATATGACTTTACGGAAGAGAGACTCTGTCTCTTCGACCGTTACTTTAAATATTGGGATCAGACATACCACCCCAATTGTATTAACTGTTTGGATGATAGGTGTATCCTCCATTGTGCAAACTTTAATGTATTGTTTTCTACTGTGTTCCCACCTACAAGCTTTGGACCATTAGTAAGGAAAATATTTGTAGATGGTGTACCTTTTGTTGTTTCAACAGGTTACCATTTCCGTGAGTTAGGAGTTGTACATAATCAGGATGTAAACTTACATAGCTCACGTCTCAGTTTTAAGGAACTTTTAGTGTATGCCGCTGACCCAGCCATGCATGCAGCTTCTGGCAATTTATTGCTAGACAAACGCACTACATGCTTTTCAGTGGCTGCACTAACAAACAATGTCTCTTTTCAAACTGTCAAACCCGGTAATTTTAATAAGGACTTTTATGACTTTGCTGTGTCTAAAGGCTTCTTTAAGGAAGGAAGTTCTGTTGAACTAAAACACTTCTTCTTTGCTCAGGATGGCAATGCTGCTATTAGCGATTATGACTATTATCGTTATAATCTGCCAACAATGTGTGATATCAGACAACTCCTATTCGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTAATCGTAAACAATCTGGATAAATCAGCTGGATTTCCATTTAACAAATGGGGTAAGGCTAGGCTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCGTACACTAAGCGTAATGTCCTCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCAGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAGAAATTACTAAAGTCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTTTACGGTGGCTGGCATAACATGTTAAAAACTGTCTACAGTGATGTAGAAACTCCCTATCTTATGGGTTGGGACTACCCAAAATGTGACAGAGCCATGCCTAACATGCTTAGAATTATGGCTTCTCTTGTTCTTGCTCGCAAACATAGCACTTGCTGTAACTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCACAAGTATTAAGTGAGATGGTCATGTGTGGAGGCTCACTATATGTAAAACCAGGTGGAACATCATCAGGTGATGCCACAACTGCTTATGCTAATAGTGTGTTTAACATTTGTCAAGCGGTGACAGCTAATGTAAATGCACTTCTTTCAACTGATGGTAACAAGATTGCTGACAAGTACGTTCGCAACCTCCAACACAGGCTATATGAGTGTCTCTATAGAAATAGAGACGTTGATCATGAATTTGTGGATGAATTTTACGCATATTTGCGTAAGCATTTCTCCATGATGATTCTTTCTGATGATGCCGTTGTGTGCTACAATAGTAACTATGCGGCTCAAGGTTTAGTAGCTAGCATCAAGAACTTTAAAGCAGTTCTTTACTACCAAAATAATGTGTTTATGTCTGAGGCAAAATGCTGGACTGAGACTGACCTTACCAAAGGACCTCATGAATTTTGCTCTCAGCATACAATGCTAGTTAAACAAGGAGATGACTATGTGTACCTGCCTTATCCAGATCCATCAAGAATACTAGGCGCAGGCTGTTTTGTTGATGACATCGTCAAAACAGATGGTACACTTATGATTGAAAGGTTTGTGTCACTTGCGATTGATGCTTACCCACTTACTAAACACCCCAACCAGGAGTATGCTGATGTTTTCCATTTGTATTTGCAATACATTAGGAAATTACATGATGAGCTTACTGGTCACATGTTGGACATGTACTCTGTAATGCTAACTAATGATAACACATCACGGTACTGGGAACCTGAGTTTTACGAGGCCATGTACACACCACATACAATCTTGCAGGCTGTAGGTGCATGTGTATTGTGTAACTCACAGACTTCACTTCGTTGCGGCGCGTGCATTAGGAGACCGTTCCTTTGTTGCAAGTGCTGCTATGACCATGTCATATCAACATCCCACAAATTAGTGCTGTCTGTTAATCCCTATGTTTGCAATGCCCCAGGTTGTGACGTTACTGATGTAACACAACTGTACTTAGGAGGTATGAGCTACTATTGCAAGTCACACAAACCTCCCATTAGTTTCCCATTGTGTGCTAATGGTCAGGTTTTTGGTCTATATAAGAACACATGTGTAGGCAGTGACAATGTTACTGACTTTAATGCTATAGCAACATGTGATTGGACTAATGCTGGCGATTACATACTTGCCAACACTTGTACAGAGAGACTCAAGCTTTTCGCAGCTGAAACGCTCAAAGCAACTGAGGAGACATTCAAGCTATCTTATGGTATTGCCACTGTACGTGAAGTACTGTCTGATAAAGAACTTCACCTTTCATGGGAGGTGGGAAAACCAAGACCACCATTGAATAGGAATTATGTCTTTACTGGTTACCGTGTAACTAAAAATAGTAAAGTACAAATTGGAGAGTACACATTTGAAAAAGGTGACTATGGTGATGCTGTTGTGTACAGAGGTACTACAACATATAAATTGAATGTTGGTGATTACTTTGTGCTAACATCACACACAGTAATGCCACTAAGTGCACCTACACTAGTGCCTCAGGAGCACTATGTGAGAATAACTGGCTTATACCCAACTCTCAACATTTCAGAAGAGTTTTCTAGCAATGTTGCAAACTACCAGAAGGTCGGTATGCAAAAATATTCAACACTCCAGGGACCACCAGGTACCGGTAAGAGTCATTTTGCTATTGGACTTGCACTCTACTACCCGTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCTGCTGTTGATGCACTATGCGAAAAGGCATTGAAATACTTGCCTATAGACAAGTGTAGTAGAATCATACCTGCACGTGCGCGTGTGGAGTGCTTTGACAAATTCAAAGTGAATTCAACATTAGAACAGTATGTTTTCTGCACTGTAAATGCACTACCTGAAACTACTGCTGACATAGTAGTCTTTGATGAAATTTCAATGGCAACTAATTATGACTTGAGTGTCGTCAATGCTAGACTACGTGCAAAACACTACGTTTACATTGGTGACCCTGCTCAATTACCGGCGCCACGCACATTGCTTACTAAGGGCACACTTGAACCTGAATACTTTAACTCGGTGTGCAGACTCATGAAAACAATAGGTCCTGACATGTTTCTTGGAACATGTCGCCGTTGTCCTGCTGAAATTGTCGACACAGTGAGTGCTTTAGTTTATGATAATAAGCTAAAAGCACACAAAGAAAAGTCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTGATCACACATGATGTTTCATCTGCAATCAACAGGCCCCAAATAGGTGTTGTAAGAGAGTTTCTTACGCGCAACCCTGCTTGGAGAAAAGCTGTTTTCATTTCACCATATAATTCACAGAATGCAGTGGCTTCTAAAATTTTAGGATTACCCACTCAAACTGTTGACTCTTCACAGGGCTCGGAGTATGACTATGTCATATTCACACAAACCACTGAGACCGCACACTCTTGCAATGTCAACCGCTTCAATGTGGCTATCACAAGAGCAAAAATTGGCATTTTGTGCATAATGTCTGACAGAGATCTTTATGATAAGCTGCAATTTACGAGTCTGGAAGTACCACGTCGCAATGTGGCTACGTTACAAGCAGAAAATGTAACTGGACTCTTTAAGGACTGTAGTAAGATCATTACTGGTCTTCACCCTACACAGGCACCGACACACCTCAGTGTTGATACTAAATTCAAGACTGAAGGCCTCTGTGTTGACATACCAGGCATACCTAAGGACATGACCTATCGCAGACTCATCTCCATGATGGGCTTCAAAATGAACTATCAAGTCAATGGTTACCCTAATATGTTTATCACCCGTGAAGAAGCCATTCGTCACGTTCGTGCATGGATTGGTTTCGACGTTGAGGGTTGTCATGCAACAAGGGATGCTGTGGGAACTAACCTACCACTCCAATTAGGATTTTCGACAGGTGTTAACTTAGTAGCTGTACCCACTGGCTATGTTGACACTGAGAATAATACAGAGTTCACTAGAGTCAATGCAAAACCTCCTCCAGGTGACCAATTCAAACATCTTATACCACTAATGTACAAGGGCTTGCCCTGGAATGTTGTGCGTATTAAGATCGTACAAATGCTCAGTGACACGCTGAAGGGATTATCTGACAGAGTCGTGTTTGTCCTTTGGGCTCATGGCTTTGAACTTACATCAATGAAGTATTTTGTCAAGATTGGACCAGAAAGAACGTGTTGTCTGTGTGACAAGCGTGCAACTTGCTTCTCTACTTCATCTGACACTTATGCTTGCTGGAATCACTCTGTGGGTTTTGACTATGTCTACAACCCGTTTATGATTGATGTCCAGCAATGGGGTCTTACAGGTAACCTTCAGAGCAACCATGATCAACACTGTCAAGTGCATGGTAATGCTCATGTGGCTAGTTGTGATGCCATCATGACTAGATGTCTTGCAGTCCATGAGTGCTTTGTTAAGCGCGTTGATTGGTCTGTTGAATACCCGATTATTGGAGATGAACTGAAGATCAACGCAGCATGCAGAAAAGTACAGCACATGGTTGTTAAATCTGCATTGCTTGCTGATAAGTTCCCAGTTCTTCATGATATAGGAAATCCAAAGGCCATTAAATGTGTACCGCAGGCTGACGTAGAATGGAAGTTCTACGATGCTCAGCCATGCAGTGACAAGGCTTATAAGATAGAAGAACTCTTCTATTCCTATGCCACCCACCATGACAAATTCACTGATGGTGTTTGCTTGTTTTGGAACTGTAACGTTGATCGTTACCCGGCCAACGCAATTGTTTGTAGGTTTGATACAAGAGTTTTATCTAACTTAAACCTACCTGGTTGTGATGGCGGTAGTCTGTATGTAAACAAACATGCATTCCACACGCCAGCCTTTGATAAGAGTGCTTTTTCTAATCTTAAACAATTGCCCTTTTTCTATTATTCAGACAGTCCCTGTGAGTCTCATGGCAAACAGGTGGTGTCAGATATTGATTATGTTCCACTCAAATCTGCTACGTGTATAACACGGTGCAACTTAGGTGGAGCTGTTTGCAGACATCATGCAAATGAGTATAGACAATACTTAGATGCATATAACATGATGATTTCTGCCGGCTTTAGCCTCTGGATTTACAAACAGTTCGATACATATAACCTGTGGAATACCTTTACCAGGTTACAGAGTCTAGAAAATGTGGCTTACAATGTTATTAATAAAGGACACTTTGATGGACAGATTGGCGAAGTACCTGTGTCTATCATCAATAATGCTGTTTACACTAAAGTAGACGGCGTTGATGTGGAGATCTTCGAGAACAAGACAATACTTCCTGTGAATGTAGCGTTTGAGCTTTGGGCTAAACGCAACATCAAGCCAGTGCCAGAGATTAAGATACTTAACAATTTGGGTGTCGACATCGCTGCCAATACTGTTATCTGGGACTACAAAAGAGAAGCACCAGCACATGTCTCAACAATAGGTATCTGCACAATGACTGACATTGCCAAGAAACCTACTGAAAGCGCTTGTTCGTCGCTTACTGTCTTATTCGATGGTAGAGTTGAAGGACAGGTTGACTTATTCAGAAATGCACGTAATGGTGTTTTAATAACGGAAGGTTCAGTCAAGGGTTTAACACCATCAAAAGGACCTGTGCAAGCAAGTGTCAATGGAGTCACATTGATTGGAGAATCAGTAAAAACACAGTTTAATTATTTCAAGAAAGTAGATGGCATCATCCAACAATTGCCTGAAACCTACTTTACACAGAGTAGAGACTTAGAGGATTTTAAGCCCAGATCACAAATGGAAACGGACTTCCTCGAGCTCGCAATGGATGAATTCATACAGCGATACAAGCTAGAGGGCTATGCCTTCGAGCATATCGTTTATGGAGATTTCAGTCATGGACAACTAGGCGGACTTCATTTAATGATAGGTCTTGCCAAGAAATCACAAGACTCACCGTTAAAATTAGAGGATTTTATCCCTATGGATAGTACGGTGAAGAATTATTTCATAACAGATGCTCAAACAGGTTCGTCAAAATGTGTCTGCTCTGTTATTGATCTTTTACTTGATGACTTTGTAGAAATAATAAAGTCACAAGATCTTTCAGTAGTTTCAAAAGTGGTCAAAGTCACAATAGACTATGCTGAAATATCATTTATGCTTTGGTGTAAAGATGGACATGTTGAAACCTTTTACCCAAAATTACAAGCGAGTCAGGCATGGCAACCGGGCGTTGCAATGCCCAACTTGTATAAGATGCAAAGAATGCTTCTTGAAAAATGTGACCTTCAGAATTATGGTGAAAACGCTGTTATACCAAAAGGAATAATGATGAATGTCGCAAAATATACCCAACTGTGTCAATACTTAAATACACTTACATTAGCTGTGCCTTACAACATGAGAGTCATACACTTTGGCGCTGGCTCTGACAAAGGAGTGGCGCCTGGTACAGCTGTGCTCAGACAGTGGTTGCCAATTGGCACACTACTTGTCGATTCTGACCTTAATGATTTTGTCTCTGACGCTGATTCTACATTAATTGGAGACTGTGCTACTGTACATACAGCTAACAAATGGGATCTCATTGTTAGCGATATGTATGACCCTAAAACTAAACATGTGACAGAGGAGAATGACTCAAAAGAAGGGTTTTTCACCTATCTGTGTGGATTTATAAAACAAAAACTAGCCCTGGGAGGTTCCGTGGCTGTAAAGATAACAGAGCATTCTTGGAATGCTGATCTCTACAAGCTTATGGGATATTTCTCATGGTGGACAGCTTTTGTCACAAATGTCAATGCGTCATCCTCTGAGGCATTTTTAATTGGAGTTAATTACCTTGGTAAGCCGAAAGAGCAAATTGATGGCTATACCATGCATGCTAACTACGTCTTCTGGAGGAATACAAATCCTATTCAATTGTCTTCTTATTCATTATTTGACATGAGCAAATTCCCTCTCAAATTAAGGGGGACTGCTGTTATGTCTTTAAAAGAGAATCAAATCAATGACATGATTTATTCCCTGTTGGAAAAGGGTAGACTTATCATTAGAGAAAACAACACAGTTGTAGTCTCAAGTGATGTTCTTGTTAATCATTAA

Protein

MESLVLGVNEKTHVQLSLPVLQVSDVLVRGFGDTVEEALAEAREHLKNGTCGLVELEKGVLRQLEEPYVFIKRSEALSTTHGHKVVELVAEMNGIQFGRSGTTLGVLVPHVGETPIAYRNVLLRKNGNKGAGGHSYGIDLKSYDLGDELNTDPIEDYEQKWNTKHGRGALRELIRELNGGAVTRYVDNNFCGPDGYPLDCIKDLLARAGKLMCTLSEQLDFIESKRGVYCCREHEHEIAWYTERSDKSYEHQTPFEIKSAKKFDTFKGECPKFVFPLNSKVKVIQPRVEKKKTEGFMGRIRSVYQVATPNECNDMHLSVFMKCNHCDEASWQTCDFLKATCEYCGTENPVSEGPTTCGYLPTNAVVKMPCPACQNKEVGPEHSVADYHNHSNIETRLRKGGRTKCFGGCVFSYVGCYNKRAYWVPRASADIGSNHTGISGDNVEVLNEDLLEILNRERVNINIVGDFQLNEEIAIILASLSASTSAFVDTVKSLDYKSFKAIVESCGNYKVTKGKPVKGAWNIGQQKSILTPLCGFPSQAAGVIRSIFSRTLDAANNSIPDLQRAAVTILGDISEQSLRLVDAMVCTSDLITNSVIIMAYVTGGLVQQTVQWLSNVLGTTVDKLKPVFTWLETKLNAGIEFLKDAWEILKFLVTGVFDIVKGQIRVFSDNLKECVKTFVGVVNKALEMCIDQVTIAGTKVRSLNLGEVFIAQSKGLYRQCVRGKEQLQLLMPLKAPKEVTFLEGDSHDTVLISEEVVLKNGELEALETPVDSFINGAVVGNPVCVNGLMLLELKDKEQYCALSPGLLATNNVFRLNGGAPVKGVTFGENTVLEVQGYKNVKITFELDERVDKVLNEKCSVYTVESGTEVTEFACVVAEAVVKTLQPVSDLLTKMGIDLDEWSVATFYLFDDAGEEKLSSRMYCSFYPPDEEEDCEEYEGEEEVPDGTCEHEYGTEDDYKGLPLEFGSSTEIQQVDDEEEDWLDDAGEAEPEPEPLPEEPVNQFTGYFKLTDNVAIKCVDIVKEAQSAKPAVIVNAANIHLKHGGGVAGALNKATNGAMQQESDDYIKRNGPLTVGGSCLLSGHNLAKKCMHVVGPNLNAGDDVQLLKAAYNNFNSQDVLLAPLLSAGIFGAKPLQSLKMCVEVVRTQVYLAVNDRSLYDQVVLDYLDSLKPKVESPKKEENPKLEEPKAKQPAEKPVDVKPKIKACVEEVTTTLEETKLLTRNLLLFADINGKLYPDSQNMLRGEDMSFLEKDAPYVVGDVITSGDITCVIIPAKKAGGTTEMLAKALKKVPVCEYITTYPGQGCAGYTLEEAKTALKRCKSAFYVLPSKTPNVKDEILGTVSWNLREMLAHAEETRKLMPICMDIRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPVASIITKLNSLNEPLITMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPDAVTTYNGYLTSSSKTPEEHFIETISLAGTYRDWSYSGQRTELGVEFLKRGDKIVYHTIEKPIEFHLDGEVLPLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSVTYGQHFGPTYLDGADVTKVKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALSHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQIEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLEFAKRVLNLVCKHCGQKTTTLTGVEAVMYMGTLSYDELKTGVSIPCVCGRGATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDGVTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNIKFADDLNQMTGFKKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPIIWHINQTTNKTTYKPNTWCLRCLWSTKPVETSNSFEVLEVEGTQGMDNLACESQPPTSEEVVENPTVQKEVIECDVKITEVVGNVILKPSDEGVKVTQELGHEDLMAAYVENTSITIKKPNELSLALGLKTLATHGAAAINSVPWSKVLAYVKPFLGQATVTTSNCMKKCVQRIFNNYMPYVITLLFQLCTFTKGTNSRIRASLPTIIAKNSVKSVAKLCLDVCIDYVKSPKSSKLFTIAMWLLLLSICLGSLIYVTAAFGVLLSNLGIPSYCGGVRDLYINSSNVTIMDFCEGSFPCSVCLSGLDSLDSYPALETIQVTISSYKLDLTFLGLAAEWFLAYMLFTKFFYLLGLSAIMQVFFGYFASHFISNSWLMWFIISIVQMAPVSAMVRMYIFFASFYYIWKSYVHIMDGCTSSTCMMCYKRNRATRVECTTIVNGMKRSFYVYANGGRGFCKAHNWNCLNCDTFCTGSTFISDEVARDLSLQFKRPINPTDQSSYVVDSVAVKNGALHLYFDKAGQKTYERHPLSLFVNLDNLRANNTKGSLPINVIVFDGKSKCEESAAKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKMFDAYVDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQGVVDTDVDTKDVIECLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGACIDCNARHINAQVAKSHNVSLIWNVKDYMSLSEQLRKQIRSAAKKNNIPFRLTCATTRQVVNVITTKISLKGGKVVSTWFKFVLKVTLLCVLSALFCYIIMPVHSLSVHDGYTNEIIGYKAIQDGVTRDIVSTDDCFANKHAGFDSWFSQRGGSYRNDKSCPVVAAIITREIGFIVPGLPGTVLRTINGDFLHFLPRVFSAVGNICYTPSKLIEYSDFATSACVLAAECTIFKDAMGKPVPYCYDTNLLEGSISYSELRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDAEYCRHGTCERSEAGICLSTSGRWVLNNEHYRALPGVFCGVDAMNLIANIFTPLVQPVGALDVSASVVAGGIIAILVTCAAYYFMKFRRAFGEYNHVVAANALLFLMSFTILCLAPAYSFLPGVYSVFYLYLTFYFTNDVSFLAHLQWFAMFSPIVPFWITAIYVFCISLKHCHWFFNNYLKRRVMFNGVTFSTFEEAALCTFLLNKEMYLKLRSETLLPLTQYNRYLALYNKYKYFSGALDTTSYREAACCHLAKALNDFSNSGADVLYQPPQTSITSAVLQSGFRRMAFPSGKVEGCMVQVTCGTTTLNGLWLDDTVYCPRHVICTAEDMLNPNYEDLLIRKSNHSFLVQAGNVQLRVIGHSMQNCLLRLKVDTSNPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNYTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGKFYGPFVDRQTAQAAGTDTTITLNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCAALKELLQNGMNGRTILGSTILEDEFTPFDVVRQCSGVTFQGKFKKIVKGTHHWMLLTFLTSLLILVQSTQWSLFFFVYENAFLPFTLGIMAIAACAMLLVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLELADTSLSGYRLKDCVMYASALVLLTLMTARTVYDDAARRVWTLMNVITLVYKVYYGNSLDQAISMWALVISVTSNYSGVVTTIMFLARAIVFVCVEYYPFLFITGNTLQCIMLVYCFLGYCCCCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQAIASEFSSLPSYAAYATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVVPDYGTYKNTCDGNTFTYASALWEIQQVVDADSKIVPLSEINMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTACTDDNALAYYNNSKGGRFVLALLSDHQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDPAKAYKDYLSSGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLRNTVCTVCGMWKGYGCSCDQLREPMMQSADASAFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNEKVAGFAKFLKTNCCRFQEKDEEGNLIDSYFVVKRHTMSNYQHEEAIYNLLKECPAVAVHDFFKFRVDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRDAGIVGVLTLDNQDLNGNWYDFGDFVQVAPGCGVPIVDSYYSLLMPILTLTRALAAESHMDADLTKPLIKWDLLKYDFTEERLCLFDRYFKYWDQTYHPNCINCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVSFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVLPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVETPYLMGWDYPKCDRAMPNMLRIMASLVLARKHSTCCNLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDHEFVDEFYAYLRKHFSMMILSDDAVVCYNSNYAAQGLVASIKNFKAVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTILQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDKELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISEEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKEKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKIGILCIMSDRDLYDKLQFTSLEVPRRNVATLQAENVTGLFKDCSKIITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATRDAVGTNLPLQLGFSTGVNLVAVPTGYVDTENNTEFTRVNAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKGLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDKRATCFSTSSDTYACWNHSVGFDYVYNPFMIDVQQWGLTGNLQSNHDQHCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWSVEYPIIGDELKINAACRKVQHMVVKSALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHHDKFTDGVCLFWNCNVDRYPANAIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFSNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRQYLDAYNMMISAGFSLWIYKQFDTYNLWNTFTRLQSLENVAYNVINKGHFDGQIGEVPVSIINNAVYTKVDGVDVEIFENKTILPVNVAFELWAKRNIKPVPEIKILNNLGVDIAANTVIWDYKREAPAHVSTIGICTMTDIAKKPTESACSSLTVLFDGRVEGQVDLFRNARNGVLITEGSVKGLTPSKGPVQASVNGVTLIGESVKTQFNYFKKVDGIIQQLPETYFTQSRDLEDFKPRSQMETDFLELAMDEFIQRYKLEGYAFEHIVYGDFSHGQLGGLHLMIGLAKKSQDSPLKLEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYAEISFMLWCKDGHVETFYPKLQASQAWQPGVAMPNLYKMQRMLLEKCDLQNYGENAVIPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPIGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIVSDMYDPKTKHVTEENDSKEGFFTYLCGFIKQKLALGGSVAVKITEHSWNADLYKLMGYFSWWTAFVTNVNASSSEAFLIGVNYLGKPKEQIDGYTMHANYVFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKENQINDMIYSLLEKGRLIIRENNTVVVSSDVLVNH

Function

Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.
Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.
Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
Proteinase 3CL-PRO: Ccleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).
Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May participate in viral replication by acting as a ssRNA-binding protein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.
Responsible for replication and transcription of the viral RNA genome.
Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.
Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.
Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H2O = ADP + H(+) + phosphate
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Biophysicochemical Properties

1.15 mM for peptide TSAVLQ/SGFRK-NH(2)
0.58 mM for peptide SGVTFQ/GKFKK
1.44 mM for peptide ATVRLQ/AGNAT

Subunit

Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.

Miscellaneous

Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Similarity

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

3D-structure Activation of host autophagy by virus ATP-binding Decay of host mRNAs by virus Endonuclease Eukaryotic host gene expression shutoff by virus Eukaryotic host translation shutoff by virus Exonuclease Helicase Host cytoplasm Host gene expression shutoff by virus Host membrane Host mRNA suppression by virus Host-virus interaction Hydrolase Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Inhibition of host ISG15 by virus Inhibition of host NF-kappa-B by virus Membrane Metal-binding Methyltransferase Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Nuclease Nucleotide-binding Nucleotidyltransferase Protease Reference proteome Repeat Ribosomal frameshifting RNA-binding RNA-directed RNA polymerase Thiol protease Transferase Transmembrane Transmembrane helix Ubl conjugation pathway Viral immunoevasion Viral RNA replication Zinc Zinc-finger

Feature

chain Host translation inhibitor nsp1

Uniprot

P0C6X7

Pubmed

12730500 12730501 12853594 12958366 12876307 12781537 + More
14527350 14645828 12917423 12917450 14561748 15331731 15564471 16549795 16882730 17024178 17692280 17855519 18417574 18827877 19369340 19640993 19153232 20573827 20421945 22174690 21345958 22039154 23035226 22615777 22791111 22635272 23943763 24991833 12746549 12925794 15007178 14962394 16228002 16271890 16581910 16873246 16873247 17202208

EMBL

AY278741 AY274119 AY278554 AY282752 AY304495 AY304486 + More
AY304488 AY278491 AY283794 AY283795 AY283796 AY283797 AY283798 AY286320 AY278488 AY278489 AY278490 AY279354 AY291451 AY310120 AY291315 AY323977 AY321118 AY338174 AY338175 AY348314 AP006557 AP006558 AP006559 AP006560 AP006561 AY427439 AY322205 AY322206 AY322207 AY463059 AY269391 AY268049

Proteomes

UP000000354 UP000103670 UP000109640 UP000116947 UP000121636 UP000131569 + More
UP000131955 UP000137377 UP000138690 UP000143093 UP000145651 UP000146108 UP000146181 UP000146296 UP000148194 UP000153467 UP000160648 UP000164441 UP000172416

PRIDE

P0C6X7

Pfam

PF08717   nsp8
PF08716   nsp7
PF12379   DUF3655
PF16251   NAR
PF01661   Macro
PF06471   NSP11
PF11633   SUD-M
PF11501   Nsp1
PF05409   Peptidase_C30
PF08715   Viral_protease
PF06460   NSP16
PF08710   nsp9
PF09401   NSP10
PF12124   Nsp3_PL2pro
PF06478   Corona_RPol_N
PF16348   Corona_NSP4_C

Interpro

IPR014827   Viral_protease
IPR013016   Peptidase_C30/C16
IPR038123   NSP4_C_sf
IPR037230   NSP8_sf
IPR042515   Nsp15_N
IPR027417   P-loop_NTPase
IPR002589   Macro_dom
IPR009461   Coronavirus_NSP16
IPR038166   Nsp3_PL2pro_sf
IPR037227   EndoU-like
IPR014829   NSP8
IPR021590   NSP1
IPR009003   Peptidase_S1_PA
IPR014822   NSP9
IPR024358   SARS-CoV_Nsp3_N
IPR038083   R1a/1ab
IPR038030   NSP1_sf
IPR036499   NSP9_sf
IPR022733   Nsp3_PL2pro
IPR007094   RNA-dir_pol_PSvirus
IPR027351   (+)RNA_virus_helicase_core_dom
IPR029063   SAM-dependent_MTases
IPR018995   RNA_synth_NSP10_coronavirus
IPR008740   Peptidase_C30
IPR042570   NAR_sf
IPR037204   NSP7_sf
IPR009469   RNA_pol_N_coronovir
IPR032505   Corona_NSP4_C
IPR038400   Nsp3_coronavir_sf
IPR009466   NSP11
IPR036333   NSP10_sf
IPR032592   NAR_dom
IPR027352   CV_ZBD
IPR014828   NSP7
IPR024375   Nsp3_coronavir

SUPFAM

SSF52540   SSF52540
SSF101816   SSF101816
SSF144246   SSF144246
SSF159936   SSF159936
SSF53335   SSF53335
SSF140367   SSF140367
SSF142877   SSF142877
SSF143076   SSF143076
SSF50494   SSF50494
SSF160099   SSF160099

Gene 3D

1.10.150.420 1.10.8.370 2.20.25.360 2.30.30.590 2.40.10.250 2.40.10.290 3.10.20.350 3.40.220.20 3.40.50.11020

ProteinModelPortal

P0C6X7

PDB

6NUS E-value=0.0 Score=1949 Identity=98.61% Cov(Q)=13.20% Cov(P)=97.70%

3D Structure

View Sequence and Structure Alignments

GO

From MSLVP

From Uniprot

Host membrane
Host cytoplasm
   nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).   With evidence from 32 publications.
Host endoplasmic reticulum-Golgi intermediate compartment   The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.   With evidence from 32 publications.

Length:

7068

Number of predicted TMHs:

14

Exp number of AAs in TMHs:

366.505469999997

Exp number, first 60 AAs:

0.0002

Total prob of N-in:

0.00005

outside

1 - 2203

TMhelix

2204 - 2226

inside

2227 - 2308

TMhelix

2309 - 2331

outside

2332 - 2335

TMhelix

2336 - 2358

inside

2359 - 2743

TMhelix

2744 - 2766

outside

2767 - 3014

TMhelix

3015 - 3037

inside

3038 - 3049

TMhelix

3050 - 3072

outside

3073 - 3099

TMhelix

3100 - 3122

inside

3123 - 3549

TMhelix

3550 - 3572

outside

3573 - 3581

TMhelix

3582 - 3601

inside

3602 - 3605

TMhelix

3606 - 3628

outside

3629 - 3653

TMhelix

3654 - 3673

inside

3674 - 3679

TMhelix

3680 - 3699

outside

3700 - 3718

TMhelix

3719 - 3741

inside

3742 - 3753

TMhelix

3754 - 3776

outside

3777 - 7068

Sequence

Summary

Ontologies

Subcellular Location

Topology