Strain
Human_TGEV_KX900405
(Region: USA: Minnesota; Strain: Transmissible gastroenteritis virus strain TGEV/USA/Minnesota148/2013, complete genome.; Date: 4-Jan-13)
Gene
replicase 1a
Description
Annotated in NCBI,
replicase 1a
Location
GenBank Accession
Full name
Replicase polyprotein 1a
Alternative Name
ORF1a polyprotein
Sequence
CDS
ATGAGTTCCAAACAATTCAAGATCCTCGTCAATGAGGACTACCAAGTCAACGTGCCTAGTCTTCCTATTCGTGACGTGTTACAGGAAATCAAGTACTGTTACCGTAATGGATTTGAGGGCTATGTTTTCGTACCAGAATACTGTCGTGACCTAGTTGATTGCGATCGTAAGGATCACTACGTCATTGGTGTTCTCGGTAACGGAGTAAGCGATCTTAAACCTGTTCTTCTTACCGAACCCTCCGTCATGTTGCAAGGTTTTATTGTTAGATCTAATTGCAATGGCGTTCTTGAGGACTTTGACTTTAAAATCGCTCGTACTGGCAGAGGTGTCATATATGTAGATCAATATATGTGTGGTGCTGATGGAAAACCAGTCATTGAAGGCGATTTTAAAGATTACTTCGGTGATGAAGACATAATTGAATTTGGAGGAAAGGAGTACCATTGCGCTTGGACAACTGTGCGCGATGAGAAACCGCTGAATCAGCAAACTCTCTTTACCATTCAGGAAATTCAATATAATCTGGACATTCCTCATAAGTTGCCAAACTGTGCTACTAGACATGTAGCACCACCAGTCAAAAAGAACTCTAAAATAGTTCTGTCTGAAGATTACAAGAAGCTCTATGATATCTTCGGATCACCTTTTATGGGAAATGGTGACTGTCTTAGCAAATGCTTTGACACTCTTCATTTTATCGCTGCCACTCTTAGATGCCCGTGTGGTTCTGAAAGTAGCGGTGTTGGAGATTGGACTGGGTTTAAGACTGCTTGTTGTGGTCTTTCTGGCAAAGTTAAGGGTGTCACTTTAGGTGATGTTAAGCCTGGTGATGCTGTTGTCACTAGTATGAGCGCAGGTAAGGGAGTTAAGTTCTTTGCCAATTGTGTTCTTCAATATGCTGGTGACGCTGAAGGTGTCTCTATATGGAAAGTTATTAAAACTTTTACAGTTGATGAGACCGTGTGCACCCTTGGTTTTGAAGGCGAGTTGAACGATTTCATCAAACCTGAGAGCAAATCACTAATTGCCTGCAGTGTTAAAAGAGCATTCATTACTGGTGAGATTGATGATGCTGTACATGATTGTATCATTACAGGAAAATTGGATCTTAGTACAAACCTTTTTGGTAACGTTGGTCTATTATTCAAGAAGACTCCATGGTTTGTACAAAAGTGTGGTGCACTTTTTGTGGATGCTTGGAAAGTAGTCGAGGAGCTTTGTGGTTCACTCACACTTACATATAAGCAAATTTATGAAGTTGTAGCATCACTTTGCAATTCTGTCTTTACGATTGTAAACTACAAGCCAACATTTGTGGTTCCAAACAATCGCGTTAAAGACCTTGTAGACAAGTGTGTGAAAGTTCTTGTAAAAGCATTTGATGTTTTTACGCAGAATATTATAATAGCTGGTATTGAGGCCAAATGCTTTGTTCTTGGTGCTAAATACCTGTTGTTCAATAATGCACTTGTCAAACTTGTCAGTGTTAAAATCCTTGGCAAGAAGCAAAAGGGTCTTGAATGTGCATTCTTTGCTACTAGCTTGGTTGGTGCAACTGCTAATGTGACACCTACAAGAACTGAGACTGCCACTATCAGCTTGAACAAGGTTGATGATGTTGTAGCACCAGGAGATGGTTATATCGTCATTGTTGGTGATATGGCTTTCTACAAGAGTGGTGAATATTATTTCATGATGTCTAGTCCTAATTCTGTTCTTACTAACAATGTTTTTAAAGCAGTTAAAGTTCAATCTTATGACATCGTTTATGATGTTGATAATGATACCAAAAGCAAAATGATTGTAAAACTTGGTTCATCATTTGAATATGATGGTGATATTGATGCTGCTATTGTAAAAGTCAACGAATTACTCATTGAATTTAGGCAGCAAAGCTTGTGCTTCAGAGCTCTTAAGGACGACAAAACCATTTTTGTTGAAGCCTACTTTAAAAAGTATAAAATGCCGGCATGCCTTGCAAAACATATTGGTTTGTGGAACATCATAAAGAAAGATTCATGTAAGAAGGGTTTTCTTAATCTCTTCAATCATTTGAATGAATTGGAAGATATTAAAGAAACTAATATACAGGCTATTAAAAACATTCTTTGTCCTGATCCTCTTCTTGATCTGGATTGTGGTGCCATTTGGTACAATTGCATGCCAAGTTGCTCTGATCCCTCAGTTTTGGGGAGTGTTCAACTTTTGATTGGTAATGGTGTGAAAGTGGTTTGTGATGGTTGCAAAAGTTTTGCTAATCAACTTTCAAAAGGTTACAACAAGCTCTGTAATGCGGCTCGTAATGATATAGAGATCGGTGGTATACCATTTTCCACTTTTAAAACACCTACAAATACTTTTATTGAAATGACAGATGCTATCTATTCAGTTGTTGAACAAGGTGAGGCATTATCCTTTAGAGATGCAGATGTGCCAGTTGTAGACGATGGTACCATTGCTATTGTTGATTGGTCTGAACCCATTCTGCTTGAACCTGCTGAATATGTAAAACCAAAGAACAATGGTAATGTCATTGTTATTGCAGGTTATACATTTTATAAAGACGAGGATGAACATTTTTATCCTTATGGTTTTGGTAAAATTGTGCAGAGAATGTATAATAAAATGGGTGGTGGTGACAAAGTTGTCTCATTTTCAGACGAAGTAGATGTTCAAGAAATTGCACCTGTTACACGTGTTAAACTTGAATTCGAATTTGATAATGAAATTGTAACTGGTGTTCTTGAACGAGCTATTGGTACTAGATACACATTTATTGGTACAACTTGGGAAGAATTTGAAGAGTCTATTTCTGAAGAACTCGATGCAATTTTTGATACTTTAGCAAACCAAGGTGTCGAACTTGAAGGTTACTTCATTTATGACACTAGTGGTGGGTTTGATATAAAAAATCCAGATGGTATTATGATATCTCAGTATGATATTACTGCTGATGACAAATCAGAAGTCGGTGCATCAAGTGAAAAAGAAGAAGTTGGCTCTGCTGAAGAAAATCCTGGGGTTGAAGTCGAAGCATCTGAAGGTGCTGAAGGGATTTCTTCTCAAGAAGAGGCTGAAACAGTAGAAGTTGCAGATCTTACTTCTACAGATGAAGATGTTGATATTGTTGAAGTATCTGCTAAAGATGACCCTTGGGCTGCAGCTGTTGATGTACAAGAAGCTGAACAATTTAATCCTTCTTTACCACCTTTCAAGACAACGAATCTCAACGGAAAAATTATCCTTAAGCAAGAGGATAATAATTGTTGGATAAATGCGTGTTGCTACCAGCTTCAGGCTTTTGATTTTTTCAACAATGAAGCTTGGGAGAAATTTAAGAAAGGTGATGTCATGGACTTTGTAAATCTGTGTTATGCAGCAACAACACTAGCAAGAGGTCATTCTGGTGATGCAGATTATCTTCTTGAACTTATGCTCAATGATTATAGCACAGCCAAGATAGTACTTGCAGCTAAGTGTGGCTGTGGTGAAAAAGAAATTGTTTTGGAACGAGCTGTTTTTAAACTCACCCCACTTAAGGAGAGTTTTAGTTATGGTGTTTGTGGTGACTGTATGCAAGTCAACACCTGTAGATTCTTAAGTGTTGAAGGCTCCGGTGTTTTTGTTCATGATATATTAAGCAAGCAAACGCCAGAATCTATGTTTATTGTTAAACCTGTTATGCACGCAGTTTACACTGGCACAACTCAAAATGGCCATTACATGGTTGATGATATTGAACATGGTTATTGTGTAGATGGTATGGGTATTAAACCACTTAAGAAACGGTGTTATACATCCACATTGTTCACTAATGCCAATGTAATGACTAGAGCTGAAAAACCAAAACAAGATTTTAAAGTTGCAAAAGTAGAACAGCAACTTATAGTGGAGGATAACAAATCTTCTATTGAAAAAGAGGTTCAAAGTCCTAAAAATGATGATCTTATACTGCCATTTTACAAAGTTGGTAAACTTTCTTTTTATCAGGGTGCTTTGGATGTTTTGATCAATTTCTTGGAACCTGATGTTATTGTTAATGCTGCTAATGGTGATCTTAAACACATGGGTGGTGTTGCAAGAGCCATCGATGTTTTCACTGGTGGCAAATTAACAGAACGTTCTAAGGATTATCTTAAAAAGAACAAAGCTATTGCTCCTGGTAACGCTGTTTTCTTTGAAAATGTCATTGAGCATCTTAGTGTTTTGAATGCAGTTGGACCACGTAATGGTGACAGCCGAGTTGAAGCCAAACTTTGTAATGTTTACAAAGCAATTGCAAAGTGTGAAGGAAAAATATTAACACCACTTATTAGTGTTGGTATCTTTAATGTTAAACTTGAAACATCATTGCAGTGCTTACTTAAGACTGCGAATGACAGGGGATTGAATGTCTTCGTATATACTGACCAGGAAAGGCAAACCATTGAAAATTTCTTTGCTTGTTCTATCCCTGTCAATGTTACTGAGGACAATGTCAACCATGAACGTGTGTCTGTTTCTTTTGACAAAACATATGGTGAACAGCTTAAGGGCACCGTTGTTATCAAAGATAAAGATGTTACAAACCAGTTGCCTAGCGCATTTGATGTTGGTCAAAAAGTTGTTAAGGCTATTGATATAGATTGGCAAGCTCATTATGGTTTTCGTGATGCTGCTGCTTTTAGCGCTAGTAGTCATGATGCTTACAAATTTGAAGTTGTTACACATAATAATTTCATTGTGCATAAGCAGACTGACAACAACTGTTGGATTAATGCAATTTGTCTTGCATTACAGAGACTCAAGCCACAGTGGAAATTTCCTGGTGTTAGAGGTCTCTGGAATGAATTTCTTGAGCGTAAAACACAAGGTTTTGTACATATGTTGTATCACATTTCTGGAGTAAAGAAAGGTGAGCCAGGTGATGCTGAATTAATGTTGCATAAACTTGGTGATTTGATGGACAACGATTGTGAAATCCTCGTCACACACACTACAGCATGTGACAAGTGCGCAAAAGCAGAAAAGTTTGTTGGACCAGTGATAGCAGCACCGCTTGCAATACATGGCACTAACGAAACATGTGTGCATGGCGTTAGTGTCAATGTCAAAGTCACTCAAATTAAGGGCACTGTTGCTATTACTTCTTTGATTGGTCCTGTTATTGGAGAGGTACTAGAAGCAACTGGTTATATTTGTTATAGCGGTTCTAAAAGGAATGGTCATTACACCTACTACGATAACCGTAATGGATTAGTGGTCGATGCAGAAAAGGCTTACCATTTTAATAGAGACTTATTACAAGTCACAACAGCTATTGCAAGTAATTTCGTTGTCAAGAAACCACAAGCAGATGAAAGACCTAAGAATTGTGCCTTTAATAAGGTCATAGCATCTCCTAAGATTGTACAAGAACAAAAATTGTTGGCTATTGAAAGTGGTGCTAACTATGCTCTTACTGAATTTGGAAAATATGCTGACATGTTCTTTACGGCTGGTGATAAAATTCTTAGGTTGCTGCTTGCAGTCTTTAAATATTTGCTGGTTTTATTTATGTGTCTTAGAAGTACTAAGATGCCTAAAGTTAAAGTCAAACCGCCTTTTGCATTTAAAGATTTTGGTGCTAAGGTCAGAACTCTCAATTACATGAGACAATTGAACAAACCTTCTATTTGGCGTTATGCAAAACTAGTTTTATTGTTGATAGCAATATATAATTTCTTTTATTTGTTTGTCAGTATACCAGTAGTGCATAAATTAACATGCAATGGTGCTGTACATGCATATAAAAATTCTAGTTTTATAAAGTCAGCAGTCTGTGGCAACTCTATTTTATGCAAAGCCTGTTTGGCTTCTTATGATGAGTTGGCCGATTTTCAACATCTCCAAGTTACTTGGGATTTTAAATCTGACCCATTATGGAACAGACTGGTACAATTGTCTTACTTTGCGTTCTTGGCTGTTTTTGGTAATAACTATGTTAGGTGTTTTCTTATGTACTTTGTATCTCAGTACCTCAACATTTGGCTTTCTTATTTTGGTTATGTAGAGTACAGTTGGTTTTTGCATGTTGTCAACTTTGAATCTATCTCAGCTGAGTTTGTGATCATAGTTATAGTGGTTAAGGCAGTTCTCGCTCTTAAACATATTGTTTTTTCATGCTCAAATCCTTCTTGCAAAACGTGTTCTAGGACTGCAAGGCAGACACGCATTCCTATTCAAGTTGTTGTTAATGGTTCAATGAAGACTGTTTATGTTCATGCTAATGGTACTGGTAAATTTTGCAAGAAACACAATTTTTATTGTAGGAACTGTGAGTCTTATGGTTTTGAAAACACATTCATCTGTGACGAAATTGTACGTGATCTCAGTAATAGTGTTAAACAAACTGTTTACGCCACTGACAGATCTCATCAAGAAGTCACAAAAGTTGAATGTTCAGATGGCTTTTACAGATTTTATGTTGGTGATGAATTCACTTCATATGATTATGATGTAAAACACAAGAAATATAGTAGTCAAGAGGTTCTTAAGAACATGCTCTTGCTTGATGATTTCATTGTGTACAGTCCGTCTGGTTCTGCTCTTGCGAATGTTAGAAATGCCTGTGTTTACTTTTCACAACTTATTGGTAAGCCTATTAAGATTGTTAACAGTGATTTGCTTGAAGATCTCTCTGTAGATTTTAAAGGGGCACTTTTTAATGCTAAAAAGAATGTAATTAAGAATTCTTTCAATGTTGATGTATCAGAATGCAAAAATCTTGATGAATGTTACAAGGCTTGCAATCTTAATGTTTCATTTTCTACATTTGAAATGGCTGTTAACAATGCTCATAGATTTGGTATTCTGATTACTGATCGTTCTTTTAACAATTTCTGGCCATCAAAAATTAAGCCTGGTTCATCTGGTGTGTCGGCCATGGACATTGGTAAGTGTATGACTTCTGATGCTAAGATTGTTAATGCTAAAGTTTTAACTCAACGTGGTAAAAGTGTTGTTTGGCTTAGCCAGGATTTTGCTGCACTTAGCTCTACTGCTCAGAAAGTTTTGGTTAAAACTTTTGTAGAAGAAGGTGTCAACTTTTCACTCACATTTAATGCTGTAGGTTCAGATGATGATCTTCCTTATGAAAGATTCACTGAGTCTGTGTCTCCAAAAAGTGGTTCAGGCTTTTTTGATGTAATTACACAGCTTAAACAAATTGTGATTTTGGTTTTTGTTTTTATTTGTATTTGTGGTTTGTGCTCTGTTTACAGTGTTGCTACACAGTCCTATATTGAATCTGCTGAGGGCTATGATTACATGGTTATTAAGAATGGAATTGTTCAACCTTTTGACGATACCATTTCATGTGTTCATAACACTTATAAAGGATTCAGTGACTGGTTTAAAGCTAAGTATGGTTTTATTCCTACTTTTGGTAAATCATGTCCAATTGTTGTAGGAACTGTTTTTGATCTTGAAAATATGAGACCAATTCCTGATGTGCCTGCATATGTTTCTATTGTGGGTAGGTCTCTTGTTTTTGCTATTAATGCTGCTTTTGGTGTTACTAATATGTGTTATGATCATACTGGCAATGCAGTTAGTAAGGACTCATATTTTGATACTTGTGTGTTTAACACTGCGTGCACCACTCTTACAGGTCTTGGTGGTACAATTGTGTATTGTGCAAAGCAAGGTTTAGTTGAAGGTGCTAAGCTCTATAGCGATCTTATGCCAGACTATTACTATGAGCATGCTAGTGGTAACATGGTTAAATTGCCAGCAATTATTAGAGGACTTGGTCTACGCTTTGTGAAAACACAGGCTACAACTTATTGTAGAGTGGGAGAGTGCATTGATAGTAAAGCTGGTTTTTGCTTTGGTGGTGATAACTGGTTTGTCTACGACAATGAGTTTGGTAATGGATATATTTGTGGTAATTCTGTGCTAGGATTTTTTAAGAATGTCTTCAAACTCTTTAACTCTAACATGTCTGTGGTAGCTACATCTGGTGCGATGCTTGTTAACATTATTATTGCATGCTTAGCTATTGCAATGTGTTATGGTGTTCTTAAGTTTAAGAAGATTTTTGGTGATTGTACTTTCCTCATTGTTATGATCATTGTCACTCTTGTTGTGAATAATGTGTCTTATTTTGTCACTCAAAATACGTTCTTTATGATCATTTACGCCATTGTTTACTATTTTACAACAAGAAAGATTGCATATCCAGGCATTTTTGATGCTGGGTTTATTATTGCCTATATTAACATGGCTCCATGGTACGTGATTACCGTATATGTCTTAGTATTCCTTTATGACTCACTCCCTTCACTGTTTAAACTTAAAGTTTCGACAAATCTTTTTGAAGGCGACAAATTTGTGGGTAACTTTGAATCTGCTGCTATGGGTACTTTTGTTATTGATATGCGTTCATATGAAACTATTGTTAATTCTACTCCTATTGATAGAATTAAATCATATGCTAACAGCTTCAATAAATATAAGTACTACACAGGTTCAATGGGAGAAGCTGACTACAGACTGGCTTGCTATGCTCATCTTGGTAAAGCTCTTATGGACTATTCTGTTAATAGGACTGACATGCTTTACACACCTCCTACTGTTAGTGTTAATTCTACACTTCAGTCAGGTTTACGGAAAATGGCACAGCCTAGTGGTCTTGTAGAGCCTTGCATTGTAAGAGTCTCCTATGGTAACAATGTTCTTAATGGTTTATGGTTAGGAGATGAAGTCATTTGCCCTAGACATGTTATTGCTAGTGATACCACACGTGTTATCAACTATGAAAATGAAATGTCTAGTGTGAGACTTCACAACTTTTCAGTTTCTAAGAATAATGTATTCTTGGGTGTTGTGTCTGCCAGATACAAGGGTGTGAATCTTGTACTTAAAGTCAATCAGGTTAATCCTAATACACCAGAACATAAATTTAAGTCTATTAAAGCTGGTGAAAGTTTTAACATTTTAGCTTGTTATGAAGGATGTCCTGGCAGTGTTTATGGTGTCAACATGAGAAGTCAAGGTACCATTAAGGGGTCTTTTATAGCTGGTACTTGTGGATCGGTAGGTTATGTGTTAGAAAATGGGATTCTCTATTTTGTATACATGCATCACTTAGAACTTGGAAATGGCTCGCATGTTGGTTCCAATTTTGAAGGAGAAATGTACGGCGGCTATGAAGATCAACCTAGCATGCAATTGGAAGGTACTAATGTCATGTCATCAGATAATGTGGTTGCATTCTTATATGCCGCACTTATCAATGGTGAAAGGTGGTTTGTTACAAACACATCGATGTCATTAGAATCATACAATACATGGGCCAAAACTAACAGTTTCACAGAACTTTCCTCAACTGATGCTTTTAGCATGTTGGCTGCAAAAACTGGTCAAAGTGTTGAGAAATTACTAGATAGTATCGTAAGACTCAACAAGGGTTTTGGAGGTCGTACTATACTCTCTTATGGCTCATTGTGTGACGAGTTCACTCCGACTGAAGTCATAAGGCAAATGTACGGTGTAAATCTTCAGGCTGGTAAAGTAAAATCTTTCTTATACCCTATTATGACTGCAATGACAATTCTTTTTGCCTTTTGGCTTGAATTCTTTATGTACACACCCTTTACGTGGATTAATCCAACTTTTGTTAGTATTGTATTGGCTGTTACAACTTTGATCTCGACGTTTTTTGTTTCTGGCATTAAACATAAAATGTTGTTCTTTATGTCTTTTGTTCTTCCTAGTGTTATCCTCGTGACAGCACATAATTTGTTCTGGGACTTTTCTTACTATGAAGGTCTTCAGTCACTTGTTGAGAATACTAACACTATGTTTTTGCCTGTTGACATGCAAGGTGTCATGCTCACAGTGTTTTGTTTTATTGTGTTTGTTACATATAGTGTTAGATTCTTCACTTGCAAACAATCATGGTTTTCACTTGCTGTGACAACTATTCTTGTGATCTTTAACATGGTTAAAATCTTTGGAATATCTGATGAACAATGGAATGAAAACCAACTTTCTTTTTGCTTTGTGAACATGCTTACTATGATTGTCAGTCTTACTACAAAGGATTGGATGGTTGTCGTTGCGTCATACAGAATTGCATATTATATTGTTGTGTGTGTAATGCCATCTGCTTTTGTATCTGACTTCGGGTTTATGAAGTGTATTAGCATTGTTTACATGGCATGCGGTTATTTGTTTTGTTGCTATTATGGCATTCTCTATTGGGTTAACAGATTTACATGCATGACTTGTGGTGTTTATCAATTCACTGTGTCTGCAGCTGAACTTAAATACATGACCGCTAATAACCTCTCTGCACCTAAGAATGCATACGATGCTATGATTCTTAGTGCTAAATTGATTGGTGTTGGAGGTAAGAGAAACATCAAAATTTCAACTGTACAGTCAAAACTTACAGAGATGAAATGTACTAATGTTGTCTTGCTTGGTCTTTTGTCTAAAATGCATGTTGAATCTAATTCAAAAGAGTGGAACTATTGTGTTGGACTACATAATGAGATAAATCTTTGTGATGACCCTGAAATCGTACTTGAGAAATTGTTAGCTCTTATTGCATTTTTCTTGTCCAAACATAATACGTGTGACCTTAGCGAACTTATTGAATCGTACTTTGAGAACACCACCATACTCCAAAGTGTGGCCTCAGCTTATGCTGCGTTGCCTAGCTGGATTGCACTTGAAAAAGCCCGCGCTGATCTTGAAGAGGCTAAGAAAAATGATGTTAGCCCTCAAATTTTGAAGCAGCTTACTAAGGCATTTAACATTGCCAAGAGTGATTTTGAGCGCGAAGCATCAGTGCAAAAGAAACTTGACAAAATGGCTGAGCAGGCTGCAGCTAGTATGTATAAAGAGGCACGAGCTGTGGACAGAAAGTCTAAGATTGTTTCTGCTATGCATAGCCTACTTTTTGGTATGCTTAAGAAACTTGATATGTCCAGTGTCAACACTATTATTGACCAGGCTCGTAACGGCGTTCTACCTTTAAGTATTATTCCAGCTGCATCAGCTACAAGACTTGTTGTCATTACACCTAGCCTTGAAGTGTTTTCCAAGATTAGGCAGGAAAACAATGTTCATTATGCTGGTGCTATTTGGACCATTGTTGAAGTTAAAGATGCTAATGGTTCACATGTACATCTTAAGGAAGTTACCGCTGCCAATGAATTAAACCTTACTTGGCCATTGAGTATCACTTGTGAGAGAACCACAAAGCTACAGAACAATGAAATTATGCCAGGTAAACTTAAAGAGCGAGCTGTCAGAGCGTCATCTACTCTTGATGGTGAAGCTTTTGGCAGTGGGAAGGCTCTTATGGCTGCTGAAAGTGGAAAAAGCTTTATGTATGCATTTATAGCCTCAGACAACAACCTTAAGTATGTTAAGTGGGAGAGCAATAATGATATTATACCTATTGAACTTGAAGCTCCATTGCGTTTCTATGTTGACGGCGCTAATGGTCCGGAAGTTAAGTACTTGTACTTTGTTAAAAATTTAAACACTCTTAGACGTGGTGCCGTTCTTGGTTATATCGGTGCAACAGTCCGTTTGCAAGCTGGTAAACCTACTGAACATCCATCTAACAGTAGTTTATTGACATTGTGTGCTTTTGCACCTGATCCTGCTAAAGCATATGTTGATGCTGTTAAGAGAGGTATGCAACCAGTTAATAACTGTGTAAAAATGCTCTCAAATGGTGCTGGTAATGGTATGGCTGTTACAAACGGTGTCGAAGCTAACACGCAGCAGGACTCTTATGGTGGTGCTTCAGTCTGTATTTATTGCAGGTGTCATGTTGAACATCCTGCTATTGATGGATTGTGCCGCTATAAAGGTAAGTTTGTGCAAGTACCAACGGGCACACAAGATCCAATTCGGTTTTGTATTGAAAATGAAGTCTGTGTTGTCTGTGGTTGTTGGCTTAACAATGGTTGTATGTGCGATCGTACGTCTATGCAGAGTTCTACTGTTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGACTAG
Protein
MSSKQFKILVNEDYQVNVPSLPIRDVLQEIKYCYRNGFEGYVFVPEYCRDLVDCDRKDHYVIGVLGNGVSDLKPVLLTEPSVMLQGFIVRSNCNGVLEDFDFKIARTGRGVIYVDQYMCGADGKPVIEGDFKDYFGDEDIIEFGGKEYHCAWTTVRDEKPLNQQTLFTIQEIQYNLDIPHKLPNCATRHVAPPVKKNSKIVLSEDYKKLYDIFGSPFMGNGDCLSKCFDTLHFIAATLRCPCGSESSGVGDWTGFKTACCGLSGKVKGVTLGDVKPGDAVVTSMSAGKGVKFFANCVLQYAGDAEGVSIWKVIKTFTVDETVCTLGFEGELNDFIKPESKSLIACSVKRAFITGEIDDAVHDCIITGKLDLSTNLFGNVGLLFKKTPWFVQKCGALFVDAWKVVEELCGSLTLTYKQIYEVVASLCNSVFTIVNYKPTFVVPNNRVKDLVDKCVKVLVKAFDVFTQNIIIAGIEAKCFVLGAKYLLFNNALVKLVSVKILGKKQKGLECAFFATSLVGATANVTPTRTETATISLNKVDDVVAPGDGYIVIVGDMAFYKSGEYYFMMSSPNSVLTNNVFKAVKVQSYDIVYDVDNDTKSKMIVKLGSSFEYDGDIDAAIVKVNELLIEFRQQSLCFRALKDDKTIFVEAYFKKYKMPACLAKHIGLWNIIKKDSCKKGFLNLFNHLNELEDIKETNIQAIKNILCPDPLLDLDCGAIWYNCMPSCSDPSVLGSVQLLIGNGVKVVCDGCKSFANQLSKGYNKLCNAARNDIEIGGIPFSTFKTPTNTFIEMTDAIYSVVEQGEALSFRDADVPVVDDGTIAIVDWSEPILLEPAEYVKPKNNGNVIVIAGYTFYKDEDEHFYPYGFGKIVQRMYNKMGGGDKVVSFSDEVDVQEIAPVTRVKLEFEFDNEIVTGVLERAIGTRYTFIGTTWEEFEESISEELDAIFDTLANQGVELEGYFIYDTSGGFDIKNPDGIMISQYDITADDKSEVGASSEKEEVGSAEENPGVEVEASEGAEGISSQEEAETVEVADLTSTDEDVDIVEVSAKDDPWAAAVDVQEAEQFNPSLPPFKTTNLNGKIILKQEDNNCWINACCYQLQAFDFFNNEAWEKFKKGDVMDFVNLCYAATTLARGHSGDADYLLELMLNDYSTAKIVLAAKCGCGEKEIVLERAVFKLTPLKESFSYGVCGDCMQVNTCRFLSVEGSGVFVHDILSKQTPESMFIVKPVMHAVYTGTTQNGHYMVDDIEHGYCVDGMGIKPLKKRCYTSTLFTNANVMTRAEKPKQDFKVAKVEQQLIVEDNKSSIEKEVQSPKNDDLILPFYKVGKLSFYQGALDVLINFLEPDVIVNAANGDLKHMGGVARAIDVFTGGKLTERSKDYLKKNKAIAPGNAVFFENVIEHLSVLNAVGPRNGDSRVEAKLCNVYKAIAKCEGKILTPLISVGIFNVKLETSLQCLLKTANDRGLNVFVYTDQERQTIENFFACSIPVNVTEDNVNHERVSVSFDKTYGEQLKGTVVIKDKDVTNQLPSAFDVGQKVVKAIDIDWQAHYGFRDAAAFSASSHDAYKFEVVTHNNFIVHKQTDNNCWINAICLALQRLKPQWKFPGVRGLWNEFLERKTQGFVHMLYHISGVKKGEPGDAELMLHKLGDLMDNDCEILVTHTTACDKCAKAEKFVGPVIAAPLAIHGTNETCVHGVSVNVKVTQIKGTVAITSLIGPVIGEVLEATGYICYSGSKRNGHYTYYDNRNGLVVDAEKAYHFNRDLLQVTTAIASNFVVKKPQADERPKNCAFNKVIASPKIVQEQKLLAIESGANYALTEFGKYADMFFTAGDKILRLLLAVFKYLLVLFMCLRSTKMPKVKVKPPFAFKDFGAKVRTLNYMRQLNKPSIWRYAKLVLLLIAIYNFFYLFVSIPVVHKLTCNGAVHAYKNSSFIKSAVCGNSILCKACLASYDELADFQHLQVTWDFKSDPLWNRLVQLSYFAFLAVFGNNYVRCFLMYFVSQYLNIWLSYFGYVEYSWFLHVVNFESISAEFVIIVIVVKAVLALKHIVFSCSNPSCKTCSRTARQTRIPIQVVVNGSMKTVYVHANGTGKFCKKHNFYCRNCESYGFENTFICDEIVRDLSNSVKQTVYATDRSHQEVTKVECSDGFYRFYVGDEFTSYDYDVKHKKYSSQEVLKNMLLLDDFIVYSPSGSALANVRNACVYFSQLIGKPIKIVNSDLLEDLSVDFKGALFNAKKNVIKNSFNVDVSECKNLDECYKACNLNVSFSTFEMAVNNAHRFGILITDRSFNNFWPSKIKPGSSGVSAMDIGKCMTSDAKIVNAKVLTQRGKSVVWLSQDFAALSSTAQKVLVKTFVEEGVNFSLTFNAVGSDDDLPYERFTESVSPKSGSGFFDVITQLKQIVILVFVFICICGLCSVYSVATQSYIESAEGYDYMVIKNGIVQPFDDTISCVHNTYKGFSDWFKAKYGFIPTFGKSCPIVVGTVFDLENMRPIPDVPAYVSIVGRSLVFAINAAFGVTNMCYDHTGNAVSKDSYFDTCVFNTACTTLTGLGGTIVYCAKQGLVEGAKLYSDLMPDYYYEHASGNMVKLPAIIRGLGLRFVKTQATTYCRVGECIDSKAGFCFGGDNWFVYDNEFGNGYICGNSVLGFFKNVFKLFNSNMSVVATSGAMLVNIIIACLAIAMCYGVLKFKKIFGDCTFLIVMIIVTLVVNNVSYFVTQNTFFMIIYAIVYYFTTRKIAYPGIFDAGFIIAYINMAPWYVITVYVLVFLYDSLPSLFKLKVSTNLFEGDKFVGNFESAAMGTFVIDMRSYETIVNSTPIDRIKSYANSFNKYKYYTGSMGEADYRLACYAHLGKALMDYSVNRTDMLYTPPTVSVNSTLQSGLRKMAQPSGLVEPCIVRVSYGNNVLNGLWLGDEVICPRHVIASDTTRVINYENEMSSVRLHNFSVSKNNVFLGVVSARYKGVNLVLKVNQVNPNTPEHKFKSIKAGESFNILACYEGCPGSVYGVNMRSQGTIKGSFIAGTCGSVGYVLENGILYFVYMHHLELGNGSHVGSNFEGEMYGGYEDQPSMQLEGTNVMSSDNVVAFLYAALINGERWFVTNTSMSLESYNTWAKTNSFTELSSTDAFSMLAAKTGQSVEKLLDSIVRLNKGFGGRTILSYGSLCDEFTPTEVIRQMYGVNLQAGKVKSFLYPIMTAMTILFAFWLEFFMYTPFTWINPTFVSIVLAVTTLISTFFVSGIKHKMLFFMSFVLPSVILVTAHNLFWDFSYYEGLQSLVENTNTMFLPVDMQGVMLTVFCFIVFVTYSVRFFTCKQSWFSLAVTTILVIFNMVKIFGISDEQWNENQLSFCFVNMLTMIVSLTTKDWMVVVASYRIAYYIVVCVMPSAFVSDFGFMKCISIVYMACGYLFCCYYGILYWVNRFTCMTCGVYQFTVSAAELKYMTANNLSAPKNAYDAMILSAKLIGVGGKRNIKISTVQSKLTEMKCTNVVLLGLLSKMHVESNSKEWNYCVGLHNEINLCDDPEIVLEKLLALIAFFLSKHNTCDLSELIESYFENTTILQSVASAYAALPSWIALEKARADLEEAKKNDVSPQILKQLTKAFNIAKSDFEREASVQKKLDKMAEQAAASMYKEARAVDRKSKIVSAMHSLLFGMLKKLDMSSVNTIIDQARNGVLPLSIIPAASATRLVVITPSLEVFSKIRQENNVHYAGAIWTIVEVKDANGSHVHLKEVTAANELNLTWPLSITCERTTKLQNNEIMPGKLKERAVRASSTLDGEAFGSGKALMAAESGKSFMYAFIASDNNLKYVKWESNNDIIPIELEAPLRFYVDGANGPEVKYLYFVKNLNTLRRGAVLGYIGATVRLQAGKPTEHPSNSSLLTLCAFAPDPAKAYVDAVKRGMQPVNNCVKMLSNGAGNGMAVTNGVEANTQQDSYGGASVCIYCRCHVEHPAIDGLCRYKGKFVQVPTGTQDPIRFCIENEVCVVCGCWLNNGCMCDRTSMQSSTVDQSYLNECGVLVQLD
Summary
Function
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
Nsp9 is a ssRNA-binding protein.
Catalytic Activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
Miscellaneous
Produced by conventional translation.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
3D-structure
Activation of host autophagy by virus
Host cytoplasm
Host membrane
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Membrane
Metal-binding
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
Thiol protease
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Zinc
Zinc-finger
Feature
chain Replicase polyprotein 1a
Uniprot
Proteomes
Pfam
Interpro
IPR009003
Peptidase_S1_PA
IPR014822 NSP9
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR013016 Peptidase_C30/C16
IPR018995 RNA_synth_NSP10_coronavirus
IPR032505 Corona_NSP4_C
IPR032039 A-CoV_nsp1
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014829 NSP8
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR036499 NSP9_sf
IPR014822 NSP9
IPR037230 NSP8_sf
IPR038123 NSP4_C_sf
IPR038634 A-CoV_nsp1_sf
IPR013016 Peptidase_C30/C16
IPR018995 RNA_synth_NSP10_coronavirus
IPR032505 Corona_NSP4_C
IPR032039 A-CoV_nsp1
IPR037204 NSP7_sf
IPR008740 Peptidase_C30
IPR014828 NSP7
IPR036333 NSP10_sf
IPR014829 NSP8
IPR002589 Macro_dom
IPR014827 Viral_protease
IPR036499 NSP9_sf
SUPFAM
ProteinModelPortal
PDB
4F49
E-value=0.0 Score= 629 Identity=99.67%
Cov(Q)=7.58% Cov(P)=98.06%
3D Structure
Ontologies
GO
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0019079 P:viral genome replication
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
GO:0008242 F:omega peptidase activity
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0003723 F:RNA binding
GO:0039520 P:induction by virus of host autophagy
GO:0004197 F:cysteine-type endopeptidase activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0019079 P:viral genome replication
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0039548 P:suppression by virus of host IRF3 activity
GO:0008270 F:zinc ion binding
GO:0019082 P:viral protein processing
GO:0016021 C:integral component of membrane
GO:0033644 C:host cell membrane
Subcellular Location
From MSLVP
Capsid
From Uniprot
Host membrane
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 4 publications.
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 4 publications.
Topology
Length:
4013
Number of predicted TMHs:
18
Exp number of AAs in TMHs:
437.008829999999
Exp number, first 60 AAs:
0.0002
Total prob of N-in:
0.00027
outside
1 - 1830
TMhelix
1831 - 1849
inside
1850 - 1886
TMhelix
1887 - 1909
outside
1910 - 1971
TMhelix
1972 - 1994
inside
1995 - 2000
TMhelix
2001 - 2020
outside
2021 - 2024
TMhelix
2025 - 2047
inside
2048 - 2396
TMhelix
2397 - 2419
outside
2420 - 2491
TMhelix
2492 - 2514
inside
2515 - 2654
TMhelix
2655 - 2677
outside
2678 - 2686
TMhelix
2687 - 2709
inside
2710 - 2715
TMhelix
2716 - 2738
outside
2739 - 2741
TMhelix
2742 - 2764
inside
2765 - 3181
TMhelix
3182 - 3204
outside
3205 - 3208
TMhelix
3209 - 3231
inside
3232 - 3237
TMhelix
3238 - 3260
outside
3261 - 3279
TMhelix
3280 - 3302
inside
3303 - 3308
TMhelix
3309 - 3331
outside
3332 - 3359
TMhelix
3360 - 3382
inside
3383 - 3388
TMhelix
3389 - 3411
outside
3412 - 4013
