Strain
Bat_MH002342
(Region: China; Strain: Pipistrellus bat coronavirus HKU5; Date: 2013/6/3)
Gene
ORF1a
Description
Annotated in NCBI,
ORF1a
Location
GenBank Accession
Full name
Replicase polyprotein 1ab
Alternative Name
ORF1ab polyprotein
Sequence
CDS
ATGTCTTTCGTGGCTGGTGTGGCTCCTCAAGGTGCGAGGGGCAAGTATAGAGCAGAGCTCAACACTGAAAAAAGGACTGACCATGTGTCTCTCAAAGCGTCACTTTGTGATGCAGGAGACCTGGTTTTCAAGATCTCACCATGGTTTATGGACGGCGAAGGCGCCTATAAACATGTGAGTGAACAACTCTCTAAGGGAAGTAAGCTTCTCTTTGTCCCTCAAACTTTGAAGGGATTCATACGGCATCTCCCAGGCCCTCGTGTTTACCTGGTTGAGAGGCTGACAGGGGGTACCTATTCGGACCCATTCATGGTTAACCAGCTTGCTTACCAAAATGCAGCAGGAGAAGGTGTCATTGGCACAACCCTGCAGGGTAAGCGTGTTGGAATGTTCTTCCCATTCGATGCGGATCTTGTTACTGGAGAGTTTCAATTTCTGCTTCGTAAGAAGGGGCTTGGTGGCAATCGCTTCCGTGATGCCCCTTGGGATTACAACTGGACTCCCTACTCTGATCTTATGGATGCTCTTGAGGCTGACCCTTGTGGTAAGTACTCTCAGAGTCTTCTTAAGAAGCTCGTTGGAGGTGATTTCACCCCAATCGATCAATATATGTGTGGCAAAAATGGCAAACCCATTGCTGAATTTGCTGCTTTAACGGCTAGCGAAGGAATCACCAAGCTAGCTGATGTGGAGGCCGAAGTCAAGTCCCGTACTGACACAGACCGCTACATCGTCTTCAAGAACAAATTGTACCGGATTGTATGGAATGTGCAACGTAAGGACGTTGCGTATCAAAAGCAATCTGCCTTTACTATGAATAGTATAGTCCAATTGGACACCATGGAGGATGTCCCTAGACACTCATTCACAATCGGCTCAGAGATCCAAGTGATTGCACCCTCAACTGCGGTGCAAGCTAATGGTCATCTCAATCTCAAACAGAGACTCCTCTACGCATTTTATGGTAAGCAGGCTGTCTCTGAACCCAACTACATCTACCATTCAGCGTACGTCGATTGCACGAGCTGTGGTAAAGGTAGTTGGCTGACTGGCAATGCTGTGCAAGGTTTTGCATGTGATTGCGGAGCACATTATTGTGCTAATGACGTAGATCTGCAGTCTTCGGGCCTTGTGCGGAAGAATGCTGTCCTGCTTACTACCTGTCCCTGCAATAAGGACGGAGAATGCAAACACACCCTCTCGCAGCTTGTTAGCATGATGACTGACAAGTGTGACGTTGAGGTTGTTGGAAAAACCTTCATTCTTACTTATGGCGGCGTAATTTACGCTTACATGGGTTGCAGTGGTGGTACTATGCACTTTATTCCTCGTGCAAAGTCATGTGTTTCCAAGATTGGCGATGCCATCTTTACTGGATGCACAGGCACCTGGAGTAAGGTATGCGAAACAGCAAACCTTTTCCTCGAAAGGGCTCAGCATGCCATCAATTTTGTTAATGAGTTTGTGCTCACAGAGACTGTAGTGGCACTACTTTCTGGTACTACTTCTAGTATCGAAGAACTTCGTGACCTGTGTCGTAACGCAACGTTCGAAAAGGTCCGAGACTATTTAACACCTCGTGGCTGGATTGTCACGATGGGTTCCTACATTGAGGGTGTCATTAACGTAGGTGCAGCTGGTGTCTGCAGCGCTGCGCTTAATGCCCCGTTCATTGTTCTCTCCGGACTCGGAGAATCCTTTAAGAAGGTCGCTGCAACTCCTTGGAAATTGTGCAGCTCCCTCAGAGCAACTCTGGACTATTATGCTGAAAGCATAACTTACAGGGTTGTACCTTATGACATTCCTTGCGATGTCAATGACTTCACAGCTCTTCTGCTAGATTCGGTTATTCTAACTGGAGCTTCTGCCTACTTTGTAGCCCGCTATGTGGATGAAAAAGTAGAGCAACTCACTAACCTTGTCTTCTCTGCATGTCAGAGCGCAGTTACAGCTTTTGTCCAGACATGTATGTCTACTTACAAAGCTACTGCAAAATTCATATCTGACATGTTCACTCTAATAAAAGTAGTCTCCGAACGGCTCTACGTCTACACGTCTGTAGGCTTTGTCGTTGTCGGAGATTATTCCTCCCAGCTTCTGAAGCAATTCATGCATATCCTCTCAAAGGCCATGCAGCTGCTTCACACCACGGTTTCTTGGGCAGGTTCCAAGTTACCATCGGTTGTGTACAATGGAAGAGATTCCCTTGTATTTCCAAGTGGCACATACCATTGTGTCTCCGCCCAAGGCAGGTCTCTGCAGGATCAATTTGATCTTGTGATTCCTGGTGATCTGAGCAAGAAGCAAATTGGCATTCTCGAACCTACACCCAACTCTACCACCGTTGATAAGAAAGTCAACTCAAATGTGGTGGAAGTAGTTGTTGGTCAGTTAGAACCTACTAAGGAGCATAGTCCCGAACTAGTCGTCGGTGATTATGTGATCATTAGTAATAAGATCTTTGTGCGCAGTGTCGAGGACAGCGAAACAGTCTTCTACCCACTCTGCACAGATGGAAAGATCGTGCCTACTTTGTTCAGACTCAAAGGTGGTGCGCCTCCCAAAGGAGTTAAGTTTGGTGGTGAACAAACAAAAGAAATAACTGCAGTCAGAAGCGTATCTGTGGACTACGATGTTCATCCTGTCCTAGATGCACTGTTAGCCGGTTCTGAACTGGCCACATTCACAGTTGAGAAGGATCTACCAGTCAAGGATTTCGTGGATGTTGTGAAGGATGAAGTTATTGAGCTGCTCTCTAAATTTCTCCGTGGTTACAACGTTGACGGATTTGATCTGGAGGATTTCGCTGATACGCCTTGCTATGTCTACAATGCTGAAGGAGACCTAGCTTGGAGCTCAACCATGACCTTCTCAGTCAACCCTGTTGAGGAAGTTGAAGAGGAGTGTGGTGATGACTATGTAGAGGATGAGTACCTCTCTGAAGAAATGCTTGTCGAGGAAGATGAGAATTCCTGGGCTGCAGCTGTTGAAGCCGTCATTCCAATGGAAGACGTCCAATTAGATACCCTTGTAGCTGAAATTGATGTTTCAGAGCCAGCTGATGATGTCGCTGAACAAGCCTCTACTGAAGAAGTGGAAGTGCCTAGTGCTTGTGTGTTGGAAGCCTCTCAAGTAGCTAACGCAGCTGAGGTTGAATCATGTGAGGCAGAAGTTTCGTCTTCGATCCCCCTCCATGAGGATGCTAACGCTGCTAAGGCCAACGATTGTGCCGAAGGTGTGCCTGCGCTTGATAGTACTGAAACTGTCAGTATGCCCTCAGTTGATACGCCAGTTGGTGATGTCACACAGGATGATGCTACAAGCAGCAATGCTACTGTAATAAGTGAAGATGTTCACACAGCCACTCATTCTAAAGGACTAGTTGCGGTTCCCGAAGTTGTTCCAGAAACGGCTCTGGACACTCCGGTTGAGCGGATGAGGTCGACGAGTGAGTGTACTGTCGTTGAGACTAGCTTAAAACAGGAGACTGCTGTAATAGTCAAAAATGATTGCTCTGCCAAACCACAAAGGGTCAAAAAGCCCAAGGCAGAGAATCCTCTTAAAAACTTTAAACACATTGTCTTGAATAATGATGTTACATTAGTCTTTGGTGACGCTATTGCTGTTGCAAAAGCGACTGAAGACTGCATTCTTGTCAATGCTGCTAATACCCATTTGAAACATGGTGGTGGCATTGCCGCTGCTATTGACAGAGCATCTGGTGGGCTTGTACAAGCCGAATCAGATGATTATGTCAACTTTTACGGGCCACTCAATGTGGGTGACTCTACTCTCCTGAAAGGGCACGGCCTTGCAACTGGCATCCTTCACGTTGTAGGCCCAGACGCGCGTGCCAATCAGGACATTCAACTTCTTAAACGCTGTTATAAGGCGTTTAACAAGTACCCCTTAGTTGTGTCGCCTTTAATCTCCGCAGGAATTTTCTGCGTAGAACCAAGGGTGTCACTTGAATACTTACTCTCAGTTGTACACACCAAAACATATGTTGTGGTCAACTCAGAGAAGATTTATAATGATCTTGCTGCTCCAAAACCTCCGACTGGATTAACTTATTCACACGAGGGTTGGCGTGGCATCATTCGTAATGCAAAATCCTTTGGATTCACGTGCTTTATCTGCACTGATCAATCCGCAAACGCAAAACTTCTCAAGGGAAGGGGTGTGGATCTTACTAAGAAAACTCAGACTGTCGATGGCGTCCAGTACTATCTTTATAGTTCTAAAGACCCATTGGCGGACATAATAACTGCTGCTAATGCCTGCAAAGGCATCTGTGCCATGCCTATAGGTTATGTAACCCATGGGCTTGACTTAGCACAGGCGGGGCAACAAGTTAAGAAAATAACTGTGCCATACGTCTGTTTGCTGGCATCAAAGGACCAAGTTCCTATCTTAAATTCTGATGTTGCAGTTCAAACACCAGAACAGTCTTTCATTAACACTGTCGTTGCTAATGGGGGTTACCATTGTTGGCATCTTGTCACTGGTGAACTCATAGTTAAGGGAGTGTCATATCGAAAACTGCTAAACTGGTCAGATCAAACCATTTGCTATGCAGATAATAAGTTTTATGTGGTAAAAGGACAAATTGCCTTACCATTTGACTCACTTGAGAAGTGTCGGACCTATCTGACTTCAAGGGCCGCACAACAGAAAAACGTCGATGTTCTTGTAACAATCGATGGGGTTAATTTCAGGACTGCTGTTCTGAATAATACAACTACTTATAGGGTACAGTTAGGTTCTGTATTCTATAAGGGTTCTGACATCTCGGACACCATTCCCACTGGAAAAATGAGTGGTGAAGCTGTTTACTTGGCTGACAATTTGTCAGAAGCTGAGAAGGCTGTACTTAGTGAAGTCTATGGAACAGCTGATGCAGCATTTCTGCACAGGTATTACTCCCTCTTGGCATTGGTTAAGAGATGGAAGTATACGGTGCATGATGGTGTTAAATCCCTTAAGCTCAATAGCAACAATTGTTACGTTAATGTGACAATGTTGATGTTGGATATGCTTAAGGAGATCAAATTCATTGTACCTGCTTTACAAGCTGCGTACCTCAAACATAAGGGTGGTGACTCTACTGAATTCATTGCCCTAGTAATGGCATATGGAGATTGTACCTATGGTGAACCTGATGATGCCTCCAGACTACTGCATACCATCCTTTCCAAGGCTGAGCTTACTACTCAAGCTAAAATGGTTTGGAGACAATGGTGCAATGTCTGTGGCGTCCAAGATACTACTACTACAGGCCTCAAGGCGTGCATTTATGTAGGAATGAACTCATTAGACGAGCTTCATGCTACACATGAGGAATGCTGTCAGTGCGGTGATGTAAGAAAACGACAACTCGTTGAACACAACGCACCATGGCTGCTGTTATCAGGACTTAATGAAGCTAAGGTAATGACGCCCACATCGCAGTCGGCCGGTCCTGACCATACAGCATTTAACGTCTTTCAAGGTGTAGAGACCTCTGTGGGTCACTACTTGCATGTACGCGTGAAGGACAACCTACTATATAAGTATGACTCCGGTAGCTTGAGCAAGACTTCCGATATGAAGTGCAAGATGACCGACGTTTATTATCCTAAGCAGATGTATAGTGCAGACTGCAATGTCGTAGTATACTCTCTAGATGGGAATACTTGGGCTGATGTAGACCCTGATCTGTCAGCTTTCTATATGAAGGATGGCAAGTACTTCACTAAGAAGCCTGTCATTGAGTATTCACCAGCTACAATTCTATCTGGTAGTGTATATACTAATAGTTGCCTTGTCGGACATGATGGTACAATTGGCAGTGATGCTATTAGTTCTTCTTTCAATAACCTATTAGGCTTTGATAATTCTAAGCCAGTGTCAAAGAAACTCACGTATTCCTTCTTCCCGGATTTCGAGGGAGATGTGATTCTTACAGAGTATTCTGCTTATGACCCCATATACAAGAATGGCGCAATGCTACATGGCAAGCCTATCTTATGGGTTAACAATTCTAAATTCGACAGTGCACTTAACAAGTTTAATAGAGCTACGCTTAGACAAGTCTATGACATAGCACCTGTTACTCTTGAGAACAAATTCACTGTCCTTCAAGACAACCAAATACAGCAGGTTGAGGTTGAAGCACCAAAGGAGGATGCTAAACCACAATCGCCTGTTCAGGTTGCAGAAGAAACAGATAATAAGCTACCCATTATCAAGTGCAAAGGTCTTAAAAAGCCATTTGTAAAGGATGGATATAGCTTTGTTAATGACCCTCAGGGTGTCAATGTTGTAGACACACTTGGTATTGATGATCTACGTGCGTTATACGTGGACCGCAGTCTGCGATTAATTGTTCTTAAAGAGAATAATTGGTCTGCACTATTTAATATACACACTGTAGAAAAGGGAGATCTCAGTGTAATTGCTGCATCAGGATCCATTACAAGAAGAGTGAAAATTCTTCTAAGTGCATCCTCTCTTTTTGCACAATTTGCATCTGTTACGGTCAATGTCACCACTGCGATGGGGAAAGCTCTAGGTAGAAGGACTCGTAATGTAATTAATAATACTGGCATTATTGGACAGGGCTTTGCTCTGCCCAAAATGTTATTAATATTACCTTTCACATTCTGGAAGTCTAAAAACCAAAGCACCGTTAAAGTTGAAGTAGGTGCTCTAAGAACTGCTGGCATTGTCACGACTAATGTTGTAAAACAGTGTGCCTCAGCAGCATATGATATGTTAGTAGTCAAGTTTAAGCGTATTGACTGGAAATCCACTTTACGCCTGCTTTTCCTTATATGTACAACTGGGCTATTGTTGTCTTCACTATATTACTTGTTCTTGTTTCATCAAGTCTTAACAAGTGACGTCATGTTAGATGGTGCCGAAGGCATGTTAGCTACTTATAGGGGGTTGCGTTCCTATTTGGGCATCCATTCTCTCTGCGACGGTATGGTCGAAGCGTATCGGAACGTCTCGTATGACGTAAACGATTTTTGCTCAAACCGTTCAGCTCTATGTAACTGGTGTCTTATTGGACAGGACTCACTTACTCGGTATTCCGCGTTCCAGATGATCCAAACCCATGTTACCAGCTATGTCATTAACATAGACTGGGTGTGGTTTGTAATGGAATTCGCACTTGCTTATGTCTTGTACACTTCAACCTTTAACGTGTTGTTACTGGTGGTGTCATCTCAGTACTTCTTCTCCTACACCGGGGCATTTGTAAACTGGCGTTCATACAATTACCTGGTCTCTGGATATTTCTTTTGCGTTACACACATCCCTTTGTTGGGTCTTGTGCGTATCTATAATTTCCTCGCATGCCTCTGGTTCCTGCGCCGATTCTACAACCATGTTATTAATGGTTGCAAGGACACTGCATGCCTGCTCTGTTATAAAAGGAATCGTCTAACACGTGTGGAAGCATCAACAGTCGTATGTGGTTCTAAACGCACGTTCTACATCGTTGCAAATGGCGGTACCTCATTCTGTTGTAGGCATAACTGGAATTGTGTTGATTGTGACACCGCAGGTATCGGGAATACCTTTATCTGTGAAGAAGTTGCAAATGACCTCACTACCAGCCTACGGAGATTAGTGAAACCGACTGACAAGTCCCATTATTATGTGGAGTCAGTTACGGTTAAAGATTCAGTTGTTCAACTGCATTGCAGTAGAGAGGGTGCCTCATGCTATGAGCGTTACCCTCTCTGCTACTTTACTAATTTAGACAAGTTGAAGTTCAAAGAGGTCTGCAAAACTCCCACGGGGATACCTGAGCACAACTTTCTAATTTATGACTCGAGTGATCGTGGCCAGGAAAATTTAGCCAGATCAGCTTGCGTTTATTATTCCCAGGTTCTCTCCAAGCCCATGTTGCTTGTAGATTCGAACATGGTCACCACTGTTGGCGACAGTCGTGAGATTGCCAGCAAAATGCTTGATTCCTATGTTAATAGCTTCATTTCTCTTTTTGGCGTGAATCGCGACAAGTTGGATAAACTTGTTGCTACCGCCCGCGATTGCGTAAAGAGAGGTGATGACTTTCAAACTGTTATTAAGACTTTCACTGATGCCGCTCGTGGTCCAGCTGGTGTGGAATCTGATGTAGAAACCTCTTCCATTGTCGATGCCTTGCAGTATGCTCATAAGCATGACCTGCAGTTGACTACGGAGGGATTTAATAATTATGTTCCTAGCTATATTAAGCCTGACAGCGTCGCGACAGCTGATCTGGGTTGTTTAATTGACCTTAACGCTGCTTCTGTCAACCAGACTAGCATCCGCAATGCAAATGGCGCATGCATCTGGAATAGCAGCGACTATATGAAACTGTCTGATAGCCTCAAGCGACAGATTAGAATCGCTTGTCGTAAGTGCAATATTTCATTTAGACTTACTACGTCTAGATTACGTTCTGTGGACAACATTTTGTCTGTTAAATTTAGTGCTACCAAGCTCTCGGGAGGTGCACCTAGATGGCTTTTGAAACTCCGTGACTTCACGTGGAAAAGTTATTGTGTAGTTACATTAGTAATCTTTGCTATGGCTGTGTTGTCTTACCTATGTTTACCGGCATTCAACATGAGTCAAGTTGCATTCCATGAGGACCGCATTCTCACTTATAAAGTTATAGAGAATGGCATCATTAGGGACATTACTCCTAGTGACGCGTGCTTCGCTAATAAGTACCAGTCCTTTTCTAAGTCATTTAACGAGAATCATGGTGGATTGTTTCAAAACTCCATTGATTGTCCCGTTACCGTAGCTGTTATCGCTGGTGTTGCAGGTACTCGTGTACCCAACGTTCCTGCTAATGTTGCATGGGTCGGTCGCCAAATAGTCTTGTTTGTCTCCCGAGTCTTTGCCTCGTCTAATAACGTCTGCTACACTCCTACCGCTGAAATCCCTTACGAGAGATTCTCCGATAGTGGGTGCGTCCTAGCATCTGAATGCACATTGTTCAGGGATGCAGAAAGTAAGATTAACCCTTACTGCTATGACCCTACTGTACTGCCTGGTGCTTCTGCGTATGATCAAATGAAGCCCCATGTTAGGTATGACATGTATGATTCAGACATGTACATAAAGTTTCCAGAAGTGGTCTTTGAGAGCACTCTGCGTATTACTAAGACCTTGGCTACTCGCTATTGTCGTTTTGGCAGTTGTGAAGATGCTAATGAAGGTGTCTGTATTACTACTAATGGCTCTTGGGCTATCTATAATGAGCACTACGCAAATAAACCAGGCGTTTATTGTGGTGATAATTACTTTGATATTGTCAGAAGACTCGGATTATCGTTGTTTCAGCCTGTCACCTACTTTCAACTTAGTACTTCGTTGGCTTTGGGTGTCATGCTTTGTATCTTCTTGACAATTGCTTTCTACTACGTCAACAAGGTCAAACGTGCCCTTGCTGATTACACACAGTGTGCAGTTGTTGCTGTAGCTGCCGCTCTTCTTAATAGCTTATGTCTATGCTTTGTTGTGTCAAACCCACTACTTGTGTTGCCATACACAGCATTGTACTACTATGCCACTTTCTACCTTACTGGTGAGCCTGCATTTGTTATGCACGTCTCATGGTTTGTCATGTTTGGAACAGTTGTCCCTATATGGATGGTGTTCGCCTACATTGTGGGAGTATGTCTTCGTCATTTACTCTGGGTTATGGCTTACTTCAGTAAGAAACACGTTGAAGTTTTTACTGATGGTAAACTGAATTGTAGTTTCCAAGATGCAGCCGCTAATATCTTTGTGATTAACAAGGACACATACGTGGCCCTCCGGAATTCTATTACCCAAGATTCCTATAATAGATACTTGTCCATGTTTAATAAGTACAAGTATTATTCTGGTGCGATGGACACTGCTTCTTACAGGGAAGCCTCTGCCGCGCATTTGTGTAAAGCTCTCCAAGTCTACAGCGAAACTGGTAGTGATGTCCTCTTCCAGCCACCCAATTGCAGTGTCACCTCAAGTGTGTTGCAGAGTGGTCTGGTTAAGATGGCTGCTCCCAGTGGAGTTGTAGAGAATTGTGTGGTTCAGGTGGCTTGCGGTTCAATGACACTTAATGGCTTATGGCTCGACAATTATGTCTGGTGCCCTCGTCATGTTATGTGTCCCGCTGATCAGTTATCTGACCCCAATTATGATGCTCTTCTTGTTTCAAAAACTAACCTTAGTTTCGTCGTTCAGAAGAACGTTGGTGCACCAGCTAACTTACGGGTTGTTGGACACACTATGGTTGGTACTCTTCTAAAGTTGACTGTGGAATCTGCTAACCCGCAGACACCCGCATACACTTTTACTACTGTAAAACCAGGAGCGTCCTTCAGTGTCTTAGCCTGTTACAATGGCAGACCAACTGGAGTTTTCATGGTAAATATGCGCCAGAATAGCACCATAAAAGGATCTTTCTTAACTGGAGTTTTCATGGTAAATATGCGCCAGAATAGCACCATAAAAGGATCTTTCTTATGTGGCTCTTGTGGTAGTGTTGGTTACACTCAAGAAGGAAATGTGATCAACTTTTGCTATATGCACCAGATGGAGTTATCAAATGGTACTCACACTGGTTGCTCTTTTGACGGTGTCATGTACGGAGCTTTTGAAGATCGTCAAGTACACCAAGTCCAATTGTCAGACAAATACTGTACTATAAATATAGTAGCTTGGCTCTATGCGGCGATTCTTAACGGTTGCAATTGGTTTGTTAAGCCAAATAAGACTGGAATCGCTACTTTTAATGAATGGGCTACGAGTAACCAATTTACAGAGTTCATTGGTACACAGTCCGTTGATATGCTTGCTCATAAGACAGGTGTTTCTGTTGAGCAACTTCTCTATGCTATCCAGACATTACACAAAGGTTTCCAGGGTAAGACAATCCTTGGGAACTCAATGCTGGAAGATGAATTCACCCCTGATGATGTTAACATGCAGGTCATGGGTGTTGTCATGCAAAGTGGTTTGAAGCGTATCTCTTACGGCCTGGTACATTGGTTGCTTACAACATTGTTACTGGCATATGTAGCTACGCTGCAACTCACCAAGTTTACTATCTGGAATTACTTGTCTGATGTGATACCCCTACAACTAACTCCATTAATTTTGTGTGTAATGGCATGTGTTATGTTGACGGTAAAGCATAGACACACATTCCTTACATTGTTTCTTTTGCCCACGGCTATTTGCTTAACATATGCAAATATTGTCTACGAGCCTCAAACACCAGTTTCAAGTGCTCTGATTGCTGTTGCAAACTGGCTTAATCCTGCTAGTGTGGACATGCGCACTACTCATACAGACTTAGGGGGTTACCTCAGTCTTTGCTTTGCATTAACTGTTGTAGTCCGTAGACTCTACAGACCTAATGCCTCAAATCTGGCTTTAGCCTTAGGTAGTGCCATGGTGTGGTTCTACACTTATACTACGGGTGATTGTTCTAGTCCCCTTACCTACCTTATGTTTCTTACTACATTGACTAGTGATTACACTGTAACCGTGTTTCTTGCAGTCAATGTTGCTAAGTTTTTCGCTCGGGTTGTGTTCCTCTATGCGCCGCATACTGGTTTCATCTTCCCAGAGGTTAAACTTGTACTGCTGATGTATCTTGCTGTAGGATACTTCTGTACTGTCTATTTTGGTGTCTTCTCCTTGTTGAATCTGAAACTTCGTGTGCCACTTGGTGTCTACGATTATACAGTTTCAACCCAAGAATTTCGCTATCTCACGGGTAACGGGCTTCATGCGCCACGTAACTCTTGGGAGGCCCTTCGTCTTAATATAAAATTGATTGGCATTGGTGGTACACCTTGTATCAAGATTGCTAGTGTCCAGTCAAAACTTACTGATCTTAAATGCACATCTGTGGTTCTTCTTTCTGTTCCTCAACAGTTACACCTCGAGGCTAACAGTAAAGCTTGGGCACACTGTGTTAAGCTTCATAATGACATCTTGGCCGCGACAGACCCTACAGAGGCATTTGACAAATTTGTCTGTCTCTTTGCTACGCTCATGTCGTTTTCTGCCAATGTGGATTTAGAGGCTTTAGCAAGTGACTTGCTTGACCACCCATCGGTGTTGCAGGCTACTTTATCTGAATTCTCCCACTTAGCTAGTTATGCTGAGTTGGAAGCTGCTCAATCTTCCTACCAAAAAGCTCTTAACTCTGGTGATGCGTCGCCACAGGTTCTTAAAGCTTTGCAGAAGGCTGTTAATATTGCAAAGAATGCCTACGAGAAAGATAAGGCAGTTGCAAGAAAGTTAGAACGCATGGCTGAACAGGCTATGACGTCAATGTATAAGCAAGCACGTGCCGAGGATAAGAAGGCCAAGATTGTAAGTGCTATGCAAACTATGCTTTTTGGCATGATTAAGAAGTTGGATAACGACGTTCTTAATGGTGTCATTTCAAATGCTAGGAACGGCTGTGTGCCTCTTAGCGTTGTGCCACTTTGTGCTTCTAATAGATTGAGAGTGGTGATACCTGATATCACTATTTGGAACAAGGTTGTCACCTGGCCAAGCTTGTCTTATGCTGGTGCACTTTGGGACATTTCTCTCATAAACAATGTGGACGGTGAAGTTGTTAAGTCTTCAGATGTCATAGAGACAAATGAATCCTTGACATGGCCACTTGTTTTGGAATGCACTCGCGCTGCAAGTTCTGCTGGCACGTTGCAAAATAATGAGATCAGGCCATCTGGTCTAAAAACTATGGTTGTTAGTGCTGGTATGGATCAGGCTAACTGTAACACTAGCTCACTGGCTTATTATGAACCTGTTGAGGGTCGTAAAATGCTGATGGGTGTTCTTTCTGAAAACGCACATCTAAAGTGGGCTAAAGTTGAAGGCCGCGATGGATTTGTGAATATTGAACTTCAACCGCCGTGTAAGTTCTTAATCGCTGGTCCCAAAGGTCCAGAAGTTAGGTACTTGTACTTTGTTAAAAACCTTAACAATTTACATCGCGGACAGCTGCTAGGCCATATTGCCGCAACAGTCCGTTTACAGGCTGGTTCTAATACAGAATTTGCTATTAATTCATCAGTGCTGTCGGCTGTCACCTTCAGTGTCGATCCTGGTAAAGCATATTTAGACTTTGTGAACGCAGGTGGCGCTCCATTGACTAATTGTGTTAAGATGTTAACTCCTAAGACTGGTACTGGAATTGCAGTGTCTGTCAAACCGGAGGCTAATGCAGATCAAGATACCTATGGTGGAGCATCAGTGTGTCTATATTGCAGAGCGCATATAGAGCACCCAGATGTCACAGGTGTTAGTAAATTTAAAGGTAAGTTTGTACAGGTTCCATTACACGTTCGTGATCCTGTTGGATTCTGTTTACAAAATACCCCGTGCAATGTTTGTCAATTTTGGATAGGACATGGGTGTAATTGTGATGCTCTTCGTGGTACTACAATTCCCCAGTCCAAAGATTCCAACTTTTTAAACGAGTCCGGGGTTCTATTGTAA
Protein
MSFVAGVAPQGARGKYRAELNTEKRTDHVSLKASLCDAGDLVFKISPWFMDGEGAYKHVSEQLSKGSKLLFVPQTLKGFIRHLPGPRVYLVERLTGGTYSDPFMVNQLAYQNAAGEGVIGTTLQGKRVGMFFPFDADLVTGEFQFLLRKKGLGGNRFRDAPWDYNWTPYSDLMDALEADPCGKYSQSLLKKLVGGDFTPIDQYMCGKNGKPIAEFAALTASEGITKLADVEAEVKSRTDTDRYIVFKNKLYRIVWNVQRKDVAYQKQSAFTMNSIVQLDTMEDVPRHSFTIGSEIQVIAPSTAVQANGHLNLKQRLLYAFYGKQAVSEPNYIYHSAYVDCTSCGKGSWLTGNAVQGFACDCGAHYCANDVDLQSSGLVRKNAVLLTTCPCNKDGECKHTLSQLVSMMTDKCDVEVVGKTFILTYGGVIYAYMGCSGGTMHFIPRAKSCVSKIGDAIFTGCTGTWSKVCETANLFLERAQHAINFVNEFVLTETVVALLSGTTSSIEELRDLCRNATFEKVRDYLTPRGWIVTMGSYIEGVINVGAAGVCSAALNAPFIVLSGLGESFKKVAATPWKLCSSLRATLDYYAESITYRVVPYDIPCDVNDFTALLLDSVILTGASAYFVARYVDEKVEQLTNLVFSACQSAVTAFVQTCMSTYKATAKFISDMFTLIKVVSERLYVYTSVGFVVVGDYSSQLLKQFMHILSKAMQLLHTTVSWAGSKLPSVVYNGRDSLVFPSGTYHCVSAQGRSLQDQFDLVIPGDLSKKQIGILEPTPNSTTVDKKVNSNVVEVVVGQLEPTKEHSPELVVGDYVIISNKIFVRSVEDSETVFYPLCTDGKIVPTLFRLKGGAPPKGVKFGGEQTKEITAVRSVSVDYDVHPVLDALLAGSELATFTVEKDLPVKDFVDVVKDEVIELLSKFLRGYNVDGFDLEDFADTPCYVYNAEGDLAWSSTMTFSVNPVEEVEEECGDDYVEDEYLSEEMLVEEDENSWAAAVEAVIPMEDVQLDTLVAEIDVSEPADDVAEQASTEEVEVPSACVLEASQVANAAEVESCEAEVSSSIPLHEDANAAKANDCAEGVPALDSTETVSMPSVDTPVGDVTQDDATSSNATVISEDVHTATHSKGLVAVPEVVPETALDTPVERMRSTSECTVVETSLKQETAVIVKNDCSAKPQRVKKPKAENPLKNFKHIVLNNDVTLVFGDAIAVAKATEDCILVNAANTHLKHGGGIAAAIDRASGGLVQAESDDYVNFYGPLNVGDSTLLKGHGLATGILHVVGPDARANQDIQLLKRCYKAFNKYPLVVSPLISAGIFCVEPRVSLEYLLSVVHTKTYVVVNSEKIYNDLAAPKPPTGLTYSHEGWRGIIRNAKSFGFTCFICTDQSANAKLLKGRGVDLTKKTQTVDGVQYYLYSSKDPLADIITAANACKGICAMPIGYVTHGLDLAQAGQQVKKITVPYVCLLASKDQVPILNSDVAVQTPEQSFINTVVANGGYHCWHLVTGELIVKGVSYRKLLNWSDQTICYADNKFYVVKGQIALPFDSLEKCRTYLTSRAAQQKNVDVLVTIDGVNFRTAVLNNTTTYRVQLGSVFYKGSDISDTIPTGKMSGEAVYLADNLSEAEKAVLSEVYGTADAAFLHRYYSLLALVKRWKYTVHDGVKSLKLNSNNCYVNVTMLMLDMLKEIKFIVPALQAAYLKHKGGDSTEFIALVMAYGDCTYGEPDDASRLLHTILSKAELTTQAKMVWRQWCNVCGVQDTTTTGLKACIYVGMNSLDELHATHEECCQCGDVRKRQLVEHNAPWLLLSGLNEAKVMTPTSQSAGPDHTAFNVFQGVETSVGHYLHVRVKDNLLYKYDSGSLSKTSDMKCKMTDVYYPKQMYSADCNVVVYSLDGNTWADVDPDLSAFYMKDGKYFTKKPVIEYSPATILSGSVYTNSCLVGHDGTIGSDAISSSFNNLLGFDNSKPVSKKLTYSFFPDFEGDVILTEYSAYDPIYKNGAMLHGKPILWVNNSKFDSALNKFNRATLRQVYDIAPVTLENKFTVLQDNQIQQVEVEAPKEDAKPQSPVQVAEETDNKLPIIKCKGLKKPFVKDGYSFVNDPQGVNVVDTLGIDDLRALYVDRSLRLIVLKENNWSALFNIHTVEKGDLSVIAASGSITRRVKILLSASSLFAQFASVTVNVTTAMGKALGRRTRNVINNTGIIGQGFALPKMLLILPFTFWKSKNQSTVKVEVGALRTAGIVTTNVVKQCASAAYDMLVVKFKRIDWKSTLRLLFLICTTGLLLSSLYYLFLFHQVLTSDVMLDGAEGMLATYRGLRSYLGIHSLCDGMVEAYRNVSYDVNDFCSNRSALCNWCLIGQDSLTRYSAFQMIQTHVTSYVINIDWVWFVMEFALAYVLYTSTFNVLLLVVSSQYFFSYTGAFVNWRSYNYLVSGYFFCVTHIPLLGLVRIYNFLACLWFLRRFYNHVINGCKDTACLLCYKRNRLTRVEASTVVCGSKRTFYIVANGGTSFCCRHNWNCVDCDTAGIGNTFICEEVANDLTTSLRRLVKPTDKSHYYVESVTVKDSVVQLHCSREGASCYERYPLCYFTNLDKLKFKEVCKTPTGIPEHNFLIYDSSDRGQENLARSACVYYSQVLSKPMLLVDSNMVTTVGDSREIASKMLDSYVNSFISLFGVNRDKLDKLVATARDCVKRGDDFQTVIKTFTDAARGPAGVESDVETSSIVDALQYAHKHDLQLTTEGFNNYVPSYIKPDSVATADLGCLIDLNAASVNQTSIRNANGACIWNSSDYMKLSDSLKRQIRIACRKCNISFRLTTSRLRSVDNILSVKFSATKLSGGAPRWLLKLRDFTWKSYCVVTLVIFAMAVLSYLCLPAFNMSQVAFHEDRILTYKVIENGIIRDITPSDACFANKYQSFSKSFNENHGGLFQNSIDCPVTVAVIAGVAGTRVPNVPANVAWVGRQIVLFVSRVFASSNNVCYTPTAEIPYERFSDSGCVLASECTLFRDAESKINPYCYDPTVLPGASAYDQMKPHVRYDMYDSDMYIKFPEVVFESTLRITKTLATRYCRFGSCEDANEGVCITTNGSWAIYNEHYANKPGVYCGDNYFDIVRRLGLSLFQPVTYFQLSTSLALGVMLCIFLTIAFYYVNKVKRALADYTQCAVVAVAAALLNSLCLCFVVSNPLLVLPYTALYYYATFYLTGEPAFVMHVSWFVMFGTVVPIWMVFAYIVGVCLRHLLWVMAYFSKKHVEVFTDGKLNCSFQDAAANIFVINKDTYVALRNSITQDSYNRYLSMFNKYKYYSGAMDTASYREASAAHLCKALQVYSETGSDVLFQPPNCSVTSSVLQSGLVKMAAPSGVVENCVVQVACGSMTLNGLWLDNYVWCPRHVMCPADQLSDPNYDALLVSKTNLSFVVQKNVGAPANLRVVGHTMVGTLLKLTVESANPQTPAYTFTTVKPGASFSVLACYNGRPTGVFMVNMRQNSTIKGSFLTGVFMVNMRQNSTIKGSFLCGSCGSVGYTQEGNVINFCYMHQMELSNGTHTGCSFDGVMYGAFEDRQVHQVQLSDKYCTINIVAWLYAAILNGCNWFVKPNKTGIATFNEWATSNQFTEFIGTQSVDMLAHKTGVSVEQLLYAIQTLHKGFQGKTILGNSMLEDEFTPDDVNMQVMGVVMQSGLKRISYGLVHWLLTTLLLAYVATLQLTKFTIWNYLSDVIPLQLTPLILCVMACVMLTVKHRHTFLTLFLLPTAICLTYANIVYEPQTPVSSALIAVANWLNPASVDMRTTHTDLGGYLSLCFALTVVVRRLYRPNASNLALALGSAMVWFYTYTTGDCSSPLTYLMFLTTLTSDYTVTVFLAVNVAKFFARVVFLYAPHTGFIFPEVKLVLLMYLAVGYFCTVYFGVFSLLNLKLRVPLGVYDYTVSTQEFRYLTGNGLHAPRNSWEALRLNIKLIGIGGTPCIKIASVQSKLTDLKCTSVVLLSVPQQLHLEANSKAWAHCVKLHNDILAATDPTEAFDKFVCLFATLMSFSANVDLEALASDLLDHPSVLQATLSEFSHLASYAELEAAQSSYQKALNSGDASPQVLKALQKAVNIAKNAYEKDKAVARKLERMAEQAMTSMYKQARAEDKKAKIVSAMQTMLFGMIKKLDNDVLNGVISNARNGCVPLSVVPLCASNRLRVVIPDITIWNKVVTWPSLSYAGALWDISLINNVDGEVVKSSDVIETNESLTWPLVLECTRAASSAGTLQNNEIRPSGLKTMVVSAGMDQANCNTSSLAYYEPVEGRKMLMGVLSENAHLKWAKVEGRDGFVNIELQPPCKFLIAGPKGPEVRYLYFVKNLNNLHRGQLLGHIAATVRLQAGSNTEFAINSSVLSAVTFSVDPGKAYLDFVNAGGAPLTNCVKMLTPKTGTGIAVSVKPEANADQDTYGGASVCLYCRAHIEHPDVTGVSKFKGKFVQVPLHVRDPVGFCLQNTPCNVCQFWIGHGCNCDALRGTTIPQSKDSNFLNESGVLL
Summary
Function
The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.
Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.
Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
Proteinase 3CL-PRO: Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).
Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May participate in viral replication by acting as a ssRNA-binding protein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.
Responsible for replication and transcription of the viral RNA genome.
Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.
Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.
Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.
Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.
Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.
Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
Proteinase 3CL-PRO: Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).
Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May participate in viral replication by acting as a ssRNA-binding protein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.
Responsible for replication and transcription of the viral RNA genome.
Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.
Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.
Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.
Catalytic Activity
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H2O = ADP + H(+) + phosphate
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H2O = ADP + H(+) + phosphate
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit
Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.
Miscellaneous
Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Similarity
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
Activation of host autophagy by virus
ATP-binding
Decay of host mRNAs by virus
Endonuclease
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Exonuclease
Helicase
Host cytoplasm
Host gene expression shutoff by virus
Host membrane
Host mRNA suppression by virus
Host-virus interaction
Hydrolase
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Inhibition of host NF-kappa-B by virus
Membrane
Metal-binding
Methyltransferase
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Nuclease
Nucleotide-binding
Nucleotidyltransferase
Protease
Reference proteome
Repeat
Ribosomal frameshifting
RNA-binding
RNA-directed RNA polymerase
Thiol protease
Transferase
Transmembrane
Transmembrane helix
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
Zinc
Zinc-finger
Feature
chain Host translation inhibitor nsp1
Uniprot
Pubmed
EMBL
Proteomes
PRIDE
Pfam
Interpro
IPR036333
NSP10_sf
IPR001205 RNA-dir_pol_C
IPR027352 CV_ZBD
IPR032592 NAR_dom
IPR014828 NSP7
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR032505 Corona_NSP4_C
IPR009469 RNA_pol_N_coronovir
IPR041679 DNA2/NAM7-like_AAA
IPR038083 R1a/1ab
IPR018995 RNA_synth_NSP10_coronavirus
IPR029063 SAM-dependent_MTases
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR027417 P-loop_NTPase
IPR013016 Peptidase_C30/C16
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR037227 EndoU-like
IPR024375 Nsp3_coronavir
IPR042570 NAR_sf
IPR038400 Nsp3_coronavir_sf
IPR009466 NSP11
IPR036499 NSP9_sf
IPR007094 RNA-dir_pol_PSvirus
IPR027351 (+)RNA_virus_helicase_core_dom
IPR042515 Nsp15_N
IPR002589 Macro_dom
IPR009461 Coronavirus_NSP16
IPR014827 Viral_protease
IPR014829 NSP8
IPR001205 RNA-dir_pol_C
IPR027352 CV_ZBD
IPR032592 NAR_dom
IPR014828 NSP7
IPR008740 Peptidase_C30
IPR037204 NSP7_sf
IPR032505 Corona_NSP4_C
IPR009469 RNA_pol_N_coronovir
IPR041679 DNA2/NAM7-like_AAA
IPR038083 R1a/1ab
IPR018995 RNA_synth_NSP10_coronavirus
IPR029063 SAM-dependent_MTases
IPR038123 NSP4_C_sf
IPR037230 NSP8_sf
IPR027417 P-loop_NTPase
IPR013016 Peptidase_C30/C16
IPR009003 Peptidase_S1_PA
IPR014822 NSP9
IPR037227 EndoU-like
IPR024375 Nsp3_coronavir
IPR042570 NAR_sf
IPR038400 Nsp3_coronavir_sf
IPR009466 NSP11
IPR036499 NSP9_sf
IPR007094 RNA-dir_pol_PSvirus
IPR027351 (+)RNA_virus_helicase_core_dom
IPR042515 Nsp15_N
IPR002589 Macro_dom
IPR009461 Coronavirus_NSP16
IPR014827 Viral_protease
IPR014829 NSP8
SUPFAM
Gene 3D
ProteinModelPortal
PDB
4YOJ
E-value=9e-179 Score= 550 Identity=78.46%
Cov(Q)=7.22% Cov(P)=106.21%
3D Structure
Ontologies
KEGG
GO
GO:0016896 F:exoribonuclease activity, producing 5'-phosphomonoesters
GO:0016021 C:integral component of membrane
GO:0005524 F:ATP binding
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0033644 C:host cell membrane
GO:0039694 P:viral RNA genome replication
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0039579 P:suppression by virus of host ISG15 activity
GO:0008242 F:omega peptidase activity
GO:0039502 P:suppression by virus of host type I interferon-mediated signaling pathway
GO:0006351 P:transcription, DNA-templated
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0004519 F:endonuclease activity
GO:0008168 F:methyltransferase activity
GO:0039595 P:induction by virus of catabolism of host mRNA
GO:0039644 P:suppression by virus of host NF-kappaB transcription factor activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0003724 F:RNA helicase activity
GO:0039520 P:induction by virus of host autophagy
GO:0016021 C:integral component of membrane
GO:0005524 F:ATP binding
GO:0019082 P:viral protein processing
GO:0008270 F:zinc ion binding
GO:0036459 F:thiol-dependent ubiquitinyl hydrolase activity
GO:0033644 C:host cell membrane
GO:0039694 P:viral RNA genome replication
GO:0003723 F:RNA binding
GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0039579 P:suppression by virus of host ISG15 activity
GO:0008242 F:omega peptidase activity
GO:0039502 P:suppression by virus of host type I interferon-mediated signaling pathway
GO:0006351 P:transcription, DNA-templated
GO:0003968 F:RNA-directed 5'-3' RNA polymerase activity
GO:0003678 F:DNA helicase activity
GO:0032259 P:methylation
GO:0004519 F:endonuclease activity
GO:0008168 F:methyltransferase activity
GO:0039595 P:induction by virus of catabolism of host mRNA
GO:0039644 P:suppression by virus of host NF-kappaB transcription factor activity
GO:0039648 P:modulation by virus of host protein ubiquitination
GO:0044172 C:host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0004197 F:cysteine-type endopeptidase activity
GO:0003724 F:RNA helicase activity
GO:0039520 P:induction by virus of host autophagy
Subcellular Location
From MSLVP
From Uniprot
Host membrane
Host cytoplasm
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 1 publications.
Host cytoplasm
nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity). With evidence from 1 publications.
Host endoplasmic reticulum-Golgi intermediate compartment The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA. With evidence from 1 publications.
Topology
Length:
4500
Number of predicted TMHs:
14
Exp number of AAs in TMHs:
367.428079999998
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00890
outside
1 - 2196
TMhelix
2197 - 2216
inside
2217 - 2265
TMhelix
2266 - 2288
outside
2289 - 2380
TMhelix
2381 - 2403
inside
2404 - 2423
TMhelix
2424 - 2446
outside
2447 - 3117
TMhelix
3118 - 3140
inside
3141 - 3160
TMhelix
3161 - 3183
outside
3184 - 3202
TMhelix
3203 - 3225
inside
3226 - 3668
TMhelix
3669 - 3688
outside
3689 - 3702
TMhelix
3703 - 3722
inside
3723 - 3728
TMhelix
3729 - 3748
outside
3749 - 3778
TMhelix
3779 - 3796
inside
3797 - 3802
TMhelix
3803 - 3820
outside
3821 - 3839
TMhelix
3840 - 3862
inside
3863 - 3873
TMhelix
3874 - 3896
outside
3897 - 4500
