Strain

USA_MURINE_1997_NC_001846 (Region: USA: NY;  Strain: Mouse hepatitis virus strain MHV-A59 C12 mutant, complete genome.;  Date: 1997)

Gene

nucleocapsid protein

Description

Annotated in NCBI,  nucleocapsid protein

Location

NC_001846(+):29669-31033

GenBank Accession

NP_045302.1

Full name

Nucleoprotein      

Alternative Name

Nucleocapsid protein

CDS

ATGTCTTTTGTTCCTGGGCAAGAAAATGCCGGTGGCAGAAGCTCCTCTGTAAACCGCGCTGGTAATGGAATCCTCAAGAAGACCACTTGGGCTGACCAAACCGAGCGTGGACCAAATAATCAAAATAGAGGCAGAAGGAATCAGCCAAAGCAGACTGCAACTACTCAACCCAACTCCGGGAGTGTGGTTCCCCATTACTCCTGGTTTTCTGGCATTACCCAGTTCCAAAAGGGAAAGGAGTTTCAGTTTGCAGAAGGACAAGGAGTGCCTATTGCCAATGGAATCCCCGCTTCAGAGCAAAAGGGATATTGGTATAGACACAACCGCCGTTCTTTTAAAACACCTGATGGGCAGCAGAAGCAATTACTGCCCAGATGGTATTTTTACTATCTTGGCACAGGGCCCCATGCTGGAGCCAGTTATGGAGACAGCATTGAAGGTGTCTTCTGGGTTGCAAACAGCCAAGCGGACACCAATACCCGCTCTGATATTGTCGAAAGGGACCCAAGCAGTCATGAGGCTATTCCTACTAGGTTTGCGCCCGGCACGGTATTGCCTCAGGGCTTTTATGTTGAAGGCTCTGGAAGGTCTGCACCTGCTAGCCGATCTGGTTCGCGGTCACAATCCCGTGGGCCAAATAATCGCGCTAGAAGCAGTTCCAACCAGCGCCAGCCTGCCTCTACTGTAAAACCTGATATGGCCGAAGAAATTGCTGCTCTTGTTTTGGCTAAGCTCGGTAAAGATGCCGGCCAGCCCAAGCAAGTAACGAAGCAAAGTGCCAAAGAAGTCAGGCAGAAAATTTTAAACAAGCCTCGCCAAAAGAGGACTCCAAACAAGCAGTGCCCAGTGCAGCAGTGTTTTGGAAAGAGAGGCCCCAATCAGAATTTTGGAGGCTCTGAAATGTTAAAACTTGGAACTAGTGATCCACAGTTCCCCATTCTTGCAGAGTTGGCTCCAACAGTTGGTGCCTTCTTCTTTGGATCTAAATTAGAATTGGTCAAAAAGAATTCTGGTGGTGCTGATGAACCCACCAAAGATGTGTATGAGCTGCAATATTCAGGTGCAGTTAGATTTGATAGTACTCTACCTGGTTTTGAGACTATCATGAAAGTGTTGAATGAGAATTTGAATGCCTACCAGAAGGATGGTGGTGCAGATGTGGTGAGCCCAAAGCCCCAAAGAAAAGGGCGTAGACAGGCTCAGGAAAAGAAAGATGAAGTAGATAATGTAAGCGTTGCAAAGCCCAAAAGCTCTGTGCAGCGAAATGTAAGTAGAGAATTAACCCCAGAGGATAGAAGTCTGTTGGCTCAGATCCTTGATGATGGCGTAGTGCCAGATGGGTTAGAAGATGACTCTAATGTGTAA

Protein

MSFVPGQENAGGRSSSVNRAGNGILKKTTWADQTERGPNNQNRGRRNQPKQTATTQPNSGSVVPHYSWFSGITQFQKGKEFQFAEGQGVPIANGIPASEQKGYWYRHNRRSFKTPDGQQKQLLPRWYFYYLGTGPHAGASYGDSIEGVFWVANSQADTNTRSDIVERDPSSHEAIPTRFAPGTVLPQGFYVEGSGRSAPASRSGSRSQSRGPNNRARSSSNQRQPASTVKPDMAEEIAALVLAKLGKDAGQPKQVTKQSAKEVRQKILNKPRQKRTPNKQCPVQQCFGKRGPNQNFGGSEMLKLGTSDPQFPILAELAPTVGAFFFGSKLELVKKNSGGADEPTKDVYELQYSGAVRFDSTLPGFETIMKVLNENLNAYQKDGGADVVSPKPQRKGRRQAQEKKDEVDNVSVAKPKSSVQRNVSRELTPEDRSLLAQILDDGVVPDGLEDDSNV

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication. Induces transcription of the prothrombinase (FGL2) and elevates procoagulant activity.

Subunit

Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity). Interacts (when phosphorylated) with host DDX1; this interaction is essential to produce a full set of subgenomic and genomic RNAs (PubMed:25299332).

Similarity

Belongs to the betacoronavirus nucleocapsid protein family.

Keywords

3D-structure   ADP-ribosylation   Host Golgi apparatus   Phosphoprotein   Reference proteome   Ribonucleoprotein   RNA-binding   Transcription   Transcription regulation   Viral nucleoprotein   Virion  

Feature

chain  Nucleoprotein

Uniprot

Q66WM6   C0KYT5   C0KYR5   C0KYY3   C0KZ04   A0A1U8QWM0   + More
S5YA14   P03416   A0A2D1CIK8   S5YGB6   C3VPG2   Q9J3E2   P18447   Q83358   A0A513RBX1   Q83360   H9BZY3   D7PJY5   P18448   D4QFK6   A0A2S0SZ21   Q5ICW7   Q9WCD0   C0KYZ4   C0KYS6   C0KYU5   I7B999   C0KYV6   I7B990   P03417   Q08614   C0KYX4   A7U549   C0KYW6   P18446   Q77NL5   Q9PY96   Q02915   Q9QCZ9   Q83359   Q9J3F6   I1TMG3   P59713   C6GHS6   Q83357   V5N735   I1TMH3   A0A096XNI2   A0A2H4N014   A0A2H4MXD4   A0A096XNH5   A0A096XNH4   A0A2H4MX28   A0A3G3NHM6   A0A3G3NH02   A8R4D8   Q9DQX6   I2FKH0   A0A4P2V418   E5RPZ1   A0A2H4MWZ7   R4WKE3   A0A4P2V3N3   A0A0K2RVN9   A0A0M5NBL9   A0A142D7Z2   A0A166IK31   A0A1W6DZX0   A0A5J6NU34   Q2QKM8   A0A5J6NRV2   B7TYH5   A0A3T0IBL9   Q8JSP4   Q9QAR1   B7U2S1   C6GHR6   C6GHL9   H9AA69   Q0PL33   A0A142D800   A0A142D7Z3   B7U2R0   P26020   A0A2H4MZ40   A0A2R4STW5   A0A142D7Z0   A0A142D801   A0A166MX98   Q6W358   A3E2F7   B8YPE5   A0A5J6NS08   Q9QAR8   A0A410UD36   A0A410UD67   H9AA48   B8RIR3   A7BKC8  

Pubmed

8392595    10438865    15709029    17079303    16341254    6687635    + More
2171216    9426441    8395114    12594208    17367888    17210170    23760243    28591694    28720894    29199039    19782089    11582515    7733827    20519394    15507445    10551451    12970410    12663803    6308569    25299332    8390823    20631137    17041219    11502093    10187767    8438589    10882653    1312608    25463600    30285857    17706262    11101590    21273011    26271151    23648375    26395082    26969241    27084706    31534035    16809333    12237422    9778786    18842722    22398294    1856695    17092595    19162098    17374765   

EMBL

FJ884686    ACO72888.1    FJ647220    ACN89710.1    FJ647218    ACN89686.1    + More
FJ647225    ACN89754.1    FJ647227    ACN89774.1    AY700211    MF618252    AAU06361.1    ATN37892.1    KF268336    KF268338    KF268339    AGT17721.1    AGT17743.1    AGT17754.1    X00509    M35256    AF029248    S64884    MF618253    ATN37902.1    KF268337    AGT17732.1    FJ884687    ACO72897.1    AF208067    AAF69349.1    M35254    L37758    AAA74733.1    MH687883    QCI55924.1    L37760    JQ173883    AFD97608.1    GU593319    ADI59793.1    M35255    AB551247    BAJ04701.1    MF416379    AWB14620.1    AY771998    AAW47245.1    AF088984    FJ647226    ACN89761.1    FJ647219    ACN89697.1    FJ647221    ACN89716.1    JX169867    AFO11522.1    FJ647222    ACN89733.1    JX169866    AFO11512.1    X00990    X63538    CAA45099.1    FJ647224    ACN89747.1    EF682498    ABS87269.1    FJ647223    ACN89735.1    M35253    AF208066    AAF69338.1    AF201929    AF061835    D10760    AF207551    AF190408    AAF05706.1    L37759    AAA76578.1    AF207902    AAF68926.1    JF792616    AFG25751.1    AJ544718    FJ938068    ACT11046.1    M80644    AAA46462.1    KF850449    AHA83612.1    JF792617    AFG25761.1    KF294372    AID16664.1    KY370049    ATP66765.1    KY370048    ATP66759.1    KF294371    AID16658.1    KF294370    AID16652.1    KY370051    ATP66776.1    MH687968    AYR18603.1    MH687970    AYR18621.1    EF446615    ABP87995.1    AF251144    AB671298    BAM15655.1    LC149486    BBD43533.1    AB555559    LC061272    BAJ52884.1    BAS18850.1    KY370046    ATP66747.1    AB775893    LC061274    LC054263    BAN18702.1    BAS18870.1    BAS53429.1    LC149485    BBD43532.1    LC061273    BAS18860.1    LC054264    BAS53430.1    KT318085    AMQ23461.1    KT852998    ANA11064.1    KY967359    ARK08665.1    MN514963    QEY10637.1    DQ011855    AAY68302.1    MN514966    QEY10661.1    FJ415324    ACJ35489.1    MH810163    AZU96330.1    AF481863    AF058943    FJ425189    ACJ67006.1    FJ938067    ACT11036.1    FJ938063    ACT10989.1    JN874562    AFE48831.1    DQ811784    ABG89284.1    KT318093    AMQ23469.1    KT318086    KT318088    AMQ23462.1    AMQ23464.1    FJ425188    FJ425190    ACJ66985.1    AF058944    KY370052    ATP66782.1    MH043952    AVZ61105.1    KT318083    AMQ23459.1    KT318089    KT318090    KT318091    KT318094    KT318095    KX982264    MG757140    MG757141    MG757142    AMQ23465.1    AMQ23466.1    AMQ23467.1    AMQ23470.1    AMQ23471.1    ARD06005.1    AVI15028.1    AVI15039.1    AVI15050.1    KU127229    KY994645    MF083115    ANA52021.1    AUF40280.1    AVV64339.1    AY316299    AAQ67202.1    DQ682406    ABG78753.1    FJ556872    ACL80212.1    MN514964    MN514965    MN514967    QEY10645.1    QEY10653.1    QEY10669.1    AF058942    MH475352    QAU20980.1    MH475353    QAU20981.1    JN874560    AFE48809.1    EU999954    ACL13001.1    AB354579    BAF75637.1   

Proteomes

UP000180349    UP000098534    UP000151686    UP000119015    UP000138757    UP000113498    + More
UP000112338    UP000164259    UP000171975    UP000007192    UP000171340    UP000180350    UP000155907    UP000118088    UP000117254    UP000154289    UP000148943    UP000158592    UP000133456    UP000149957    UP000142874    UP000114687    UP000007193    UP000167944    UP000159025    UP000143567    UP000139707    UP000163097    UP000175084    UP000173347    UP000161770    UP000103372    UP000166748    UP000155347    UP000130220    UP000125924    UP000158296    UP000007543    UP000133443    UP000164707    UP000172225    UP000145001    UP000147981    UP000102696    UP000148210    UP000124585    UP000168103   

PRIDE

P03416    P18447    Q83360    P18448    P03417    P18446    + More
Q9PY96    Q9QAR8   

Pfam

PF00937   Corona_nucleoca

Interpro

IPR037195   Nucleocapsid_N
IPR001218   Corona_nucleocap
IPR037179   Nucleocapsid_C

SUPFAM

SSF103068   SSF103068
SSF110304   SSF110304

ProteinModelPortal

Q66WM6   C0KYT5   C0KYR5   C0KYY3   C0KZ04   A0A1U8QWM0   + More
S5YA14   P03416   A0A2D1CIK8   S5YGB6   C3VPG2   Q9J3E2   P18447   Q83358   A0A513RBX1   Q83360   H9BZY3   D7PJY5   P18448   D4QFK6   A0A2S0SZ21   Q5ICW7   Q9WCD0   C0KYZ4   C0KYS6   C0KYU5   I7B999   C0KYV6   I7B990   P03417   Q08614   C0KYX4   A7U549   C0KYW6   P18446   Q77NL5   Q9PY96   Q02915   Q9QCZ9   Q83359   Q9J3F6   I1TMG3   P59713   C6GHS6   Q83357   V5N735   I1TMH3   A0A096XNI2   A0A2H4N014   A0A2H4MXD4   A0A096XNH5   A0A096XNH4   A0A2H4MX28   A0A3G3NHM6   A0A3G3NH02   A8R4D8   Q9DQX6   I2FKH0   A0A4P2V418   E5RPZ1   A0A2H4MWZ7   R4WKE3   A0A4P2V3N3   A0A0K2RVN9   A0A0M5NBL9   A0A142D7Z2   A0A166IK31   A0A1W6DZX0   A0A5J6NU34   Q2QKM8   A0A5J6NRV2   B7TYH5   A0A3T0IBL9   Q8JSP4   Q9QAR1   B7U2S1   C6GHR6   C6GHL9   H9AA69   Q0PL33   A0A142D800   A0A142D7Z3   B7U2R0   P26020   A0A2H4MZ40   A0A2R4STW5   A0A142D7Z0   A0A142D801   A0A166MX98   Q6W358   A3E2F7   B8YPE5   A0A5J6NS08   Q9QAR8   A0A410UD36   A0A410UD67   H9AA48   B8RIR3   A7BKC8  

PDB

3HD4     E-value=1.63683e-72,     Score=694